Proteomes of the Female Genital Tract During the Oestrous Cycle.
Identifieur interne : 001823 ( PubMed/Checkpoint ); précédent : 001822; suivant : 001824Proteomes of the Female Genital Tract During the Oestrous Cycle.
Auteurs : Clement Soleilhavoup [France] ; Cindy Riou [France] ; Guillaume Tsikis [France] ; Valerie Labas [France] ; Gregoire Harichaux [France] ; Philippa Kohnke [France] ; Karine Reynaud [France] ; Simon P. De Graaf [Australie] ; Nadine Gerard [France] ; Xavier Druart [France]Source :
- Molecular & cellular proteomics : MCP [ 1535-9484 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Chromatographie en phase liquide, Col de l'utérus (métabolisme), Femelle, Immunohistochimie, Oestrus (métabolisme), Oviductes (métabolisme), Ovis, Protéome (métabolisme), Protéomique (), Spectrométrie de masse en tandem, Système génital de la femme (métabolisme), Technique de Western, Utérus (métabolisme).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Proteome.
- metabolism : Cervix Uteri, Estrus, Genitalia, Female, Oviducts, Uterus.
- methods : Proteomics.
- Animals, Blotting, Western, Chromatography, Liquid, Female, Immunohistochemistry, Sheep, Tandem Mass Spectrometry.
Abstract
The female genital tract includes several anatomical regions whose luminal fluids successively interact with gametes and embryos and are involved in the fertilisation and development processes. The luminal fluids from the inner cervix, the uterus and the oviduct were collected along the oestrous cycle at oestrus (Day 0 of the cycle) and during the luteal phase (Day 10) from adult cyclic ewes. The proteomes were assessed by GeLC-MS/MS and quantified by spectral counting. A set of 940 proteins were identified including 291 proteins differentially present along the cycle in one or several regions. The global analysis of the fluid proteomes revealed a general pattern of endocrine regulation of the tract, with the cervix and the oviduct showing an increased differential proteins abundance mainly at oestrus while the uterus showed an increased abundance mainly during the luteal phase. The proteins more abundant at oestrus included several families such as the heat shock proteins (HSP), the mucins, the complement cascade proteins and several redox enzymes. Other proteins known for their interaction with gametes such as oviductin (OVGP), osteopontin, HSPA8, and the spermadhesin AWN were also overexpressed at oestrus. The proteins more abundant during the luteal phase were associated with the immune system such as ceruloplasmin, lactoferrin, DMBT1, or PIGR, and also with tissue remodeling such as galectin 3 binding protein, alkaline phosphatase, CD9, or fibulin. Several proteins differentially abundant between estrus and the luteal phase, such as myosin 9 and fibronectin, were also validated by immunohistochemistry. The potential roles in sperm transit and uterine receptivity of the proteins differentially regulated along the cycle in the female genital tract are discussed.
DOI: 10.1074/mcp.M115.052332
PubMed: 26518761
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§CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Tsikis, Guillaume" sort="Tsikis, Guillaume" uniqKey="Tsikis G" first="Guillaume" last="Tsikis">Guillaume Tsikis</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valerie" last="Labas">Valerie Labas</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Harichaux, Gregoire" sort="Harichaux, Gregoire" uniqKey="Harichaux G" first="Gregoire" last="Harichaux">Gregoire Harichaux</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Kohnke, Philippa" sort="Kohnke, Philippa" uniqKey="Kohnke P" first="Philippa" last="Kohnke">Philippa Kohnke</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Reynaud, Karine" sort="Reynaud, Karine" uniqKey="Reynaud K" first="Karine" last="Reynaud">Karine Reynaud</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; ‡‡Alfort Veterinary School, 94700 Maisons Alfort, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; ‡‡Alfort Veterinary School, 94700 Maisons Alfort</wicri:regionArea><wicri:noRegion>94700 Maisons Alfort</wicri:noRegion><wicri:noRegion>94700 Maisons Alfort</wicri:noRegion></affiliation></author><author><name sortKey="De Graaf, Simon P" sort="De Graaf, Simon P" uniqKey="De Graaf S" first="Simon P" last="De Graaf">Simon P. De Graaf</name><affiliation wicri:level="1"><nlm:affiliation>§§Faculty of Veterinary Science, The University of Sydney NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>§§Faculty of Veterinary Science, The University of Sydney NSW 2006</wicri:regionArea><wicri:noRegion>The University of Sydney NSW 2006</wicri:noRegion></affiliation></author><author><name sortKey="Gerard, Nadine" sort="Gerard, Nadine" uniqKey="Gerard N" first="Nadine" last="Gerard">Nadine Gerard</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Druart, Xavier" sort="Druart, Xavier" uniqKey="Druart X" first="Xavier" last="Druart">Xavier Druart</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; Xavier.Druart@tours.inra.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26518761</idno><idno type="pmid">26518761</idno><idno type="doi">10.1074/mcp.M115.052332</idno><idno type="wicri:Area/PubMed/Corpus">002532</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002532</idno><idno type="wicri:Area/PubMed/Curation">002464</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002464</idno><idno type="wicri:Area/PubMed/Checkpoint">002464</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002464</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomes of the Female Genital Tract During the Oestrous Cycle.</title><author><name sortKey="Soleilhavoup, Clement" sort="Soleilhavoup, Clement" uniqKey="Soleilhavoup C" first="Clement" last="Soleilhavoup">Clement Soleilhavoup</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Nouzilly</settlement></placeName></affiliation></author><author><name sortKey="Riou, Cindy" sort="Riou, Cindy" uniqKey="Riou C" first="Cindy" last="Riou">Cindy Riou</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Nouzilly</settlement></placeName></affiliation></author><author><name sortKey="Tsikis, Guillaume" sort="Tsikis, Guillaume" uniqKey="Tsikis G" first="Guillaume" last="Tsikis">Guillaume Tsikis</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Nouzilly</settlement></placeName></affiliation></author><author><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valerie" last="Labas">Valerie Labas</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Nouzilly</settlement></placeName></affiliation></author><author><name sortKey="Harichaux, Gregoire" sort="Harichaux, Gregoire" uniqKey="Harichaux G" first="Gregoire" last="Harichaux">Gregoire Harichaux</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Nouzilly</settlement></placeName></affiliation></author><author><name sortKey="Kohnke, Philippa" sort="Kohnke, Philippa" uniqKey="Kohnke P" first="Philippa" last="Kohnke">Philippa Kohnke</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Nouzilly</settlement></placeName></affiliation></author><author><name sortKey="Reynaud, Karine" sort="Reynaud, Karine" uniqKey="Reynaud K" first="Karine" last="Reynaud">Karine Reynaud</name><affiliation wicri:level="3"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; ‡‡Alfort Veterinary School, 94700 Maisons Alfort, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; ‡‡Alfort Veterinary School, 94700 Maisons Alfort</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Maisons Alfort</settlement></placeName></affiliation></author><author><name sortKey="De Graaf, Simon P" sort="De Graaf, Simon P" uniqKey="De Graaf S" first="Simon P" last="De Graaf">Simon P. De Graaf</name><affiliation wicri:level="1"><nlm:affiliation>§§Faculty of Veterinary Science, The University of Sydney NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>§§Faculty of Veterinary Science, The University of Sydney NSW 2006</wicri:regionArea><wicri:noRegion>The University of Sydney NSW 2006</wicri:noRegion></affiliation></author><author><name sortKey="Gerard, Nadine" sort="Gerard, Nadine" uniqKey="Gerard N" first="Nadine" last="Gerard">Nadine Gerard</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Druart, Xavier" sort="Druart, Xavier" uniqKey="Druart X" first="Xavier" last="Druart">Xavier Druart</name><affiliation wicri:level="1"><nlm:affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; Xavier.Druart@tours.inra.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly</wicri:regionArea><wicri:noRegion>37380 Nouzilly</wicri:noRegion><wicri:noRegion>37380 Nouzilly</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular & cellular proteomics : MCP</title><idno type="eISSN">1535-9484</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cervix Uteri (metabolism)</term><term>Chromatography, Liquid</term><term>Estrus (metabolism)</term><term>Female</term><term>Genitalia, Female (metabolism)</term><term>Immunohistochemistry</term><term>Oviducts (metabolism)</term><term>Proteome (metabolism)</term><term>Proteomics (methods)</term><term>Sheep</term><term>Tandem Mass Spectrometry</term><term>Uterus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chromatographie en phase liquide</term><term>Col de l'utérus (métabolisme)</term><term>Femelle</term><term>Immunohistochimie</term><term>Oestrus (métabolisme)</term><term>Oviductes (métabolisme)</term><term>Ovis</term><term>Protéome (métabolisme)</term><term>Protéomique ()</term><term>Spectrométrie de masse en tandem</term><term>Système génital de la femme (métabolisme)</term><term>Technique de Western</term><term>Utérus (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cervix Uteri</term><term>Estrus</term><term>Genitalia, Female</term><term>Oviducts</term><term>Uterus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Col de l'utérus</term><term>Oestrus</term><term>Oviductes</term><term>Protéome</term><term>Système génital de la femme</term><term>Utérus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Chromatography, Liquid</term><term>Female</term><term>Immunohistochemistry</term><term>Sheep</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chromatographie en phase liquide</term><term>Femelle</term><term>Immunohistochimie</term><term>Ovis</term><term>Protéomique</term><term>Spectrométrie de masse en tandem</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The female genital tract includes several anatomical regions whose luminal fluids successively interact with gametes and embryos and are involved in the fertilisation and development processes. The luminal fluids from the inner cervix, the uterus and the oviduct were collected along the oestrous cycle at oestrus (Day 0 of the cycle) and during the luteal phase (Day 10) from adult cyclic ewes. The proteomes were assessed by GeLC-MS/MS and quantified by spectral counting. A set of 940 proteins were identified including 291 proteins differentially present along the cycle in one or several regions. The global analysis of the fluid proteomes revealed a general pattern of endocrine regulation of the tract, with the cervix and the oviduct showing an increased differential proteins abundance mainly at oestrus while the uterus showed an increased abundance mainly during the luteal phase. The proteins more abundant at oestrus included several families such as the heat shock proteins (HSP), the mucins, the complement cascade proteins and several redox enzymes. Other proteins known for their interaction with gametes such as oviductin (OVGP), osteopontin, HSPA8, and the spermadhesin AWN were also overexpressed at oestrus. The proteins more abundant during the luteal phase were associated with the immune system such as ceruloplasmin, lactoferrin, DMBT1, or PIGR, and also with tissue remodeling such as galectin 3 binding protein, alkaline phosphatase, CD9, or fibulin. Several proteins differentially abundant between estrus and the luteal phase, such as myosin 9 and fibronectin, were also validated by immunohistochemistry. The potential roles in sperm transit and uterine receptivity of the proteins differentially regulated along the cycle in the female genital tract are discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26518761</PMID><DateCreated><Year>2016</Year><Month>01</Month><Day>02</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>11</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-9484</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Molecular & cellular proteomics : MCP</Title><ISOAbbreviation>Mol. Cell Proteomics</ISOAbbreviation></Journal><ArticleTitle>Proteomes of the Female Genital Tract During the Oestrous Cycle.</ArticleTitle><Pagination><MedlinePgn>93-108</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1074/mcp.M115.052332</ELocationID><Abstract><AbstractText>The female genital tract includes several anatomical regions whose luminal fluids successively interact with gametes and embryos and are involved in the fertilisation and development processes. The luminal fluids from the inner cervix, the uterus and the oviduct were collected along the oestrous cycle at oestrus (Day 0 of the cycle) and during the luteal phase (Day 10) from adult cyclic ewes. The proteomes were assessed by GeLC-MS/MS and quantified by spectral counting. A set of 940 proteins were identified including 291 proteins differentially present along the cycle in one or several regions. The global analysis of the fluid proteomes revealed a general pattern of endocrine regulation of the tract, with the cervix and the oviduct showing an increased differential proteins abundance mainly at oestrus while the uterus showed an increased abundance mainly during the luteal phase. The proteins more abundant at oestrus included several families such as the heat shock proteins (HSP), the mucins, the complement cascade proteins and several redox enzymes. Other proteins known for their interaction with gametes such as oviductin (OVGP), osteopontin, HSPA8, and the spermadhesin AWN were also overexpressed at oestrus. The proteins more abundant during the luteal phase were associated with the immune system such as ceruloplasmin, lactoferrin, DMBT1, or PIGR, and also with tissue remodeling such as galectin 3 binding protein, alkaline phosphatase, CD9, or fibulin. Several proteins differentially abundant between estrus and the luteal phase, such as myosin 9 and fibronectin, were also validated by immunohistochemistry. The potential roles in sperm transit and uterine receptivity of the proteins differentially regulated along the cycle in the female genital tract are discussed.</AbstractText><CopyrightInformation>© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Soleilhavoup</LastName><ForeName>Clement</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Riou</LastName><ForeName>Cindy</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsikis</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Labas</LastName><ForeName>Valerie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Harichaux</LastName><ForeName>Gregoire</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; **INRA, Plate-forme d'Analyse Intégrative des Biomolécules (PAIB), Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kohnke</LastName><ForeName>Philippa</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reynaud</LastName><ForeName>Karine</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; ‡‡Alfort Veterinary School, 94700 Maisons Alfort, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Graaf</LastName><ForeName>Simon P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>§§Faculty of Veterinary Science, The University of Sydney NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerard</LastName><ForeName>Nadine</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Druart</LastName><ForeName>Xavier</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>From the ‡INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; §CNRS, UMR7247, F-37380 Nouzilly, France; ¶Université François Rabelais de Tours, F-37000 Tours, France; ‖IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; Xavier.Druart@tours.inra.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Cell Proteomics</MedlineTA><NlmUniqueID>101125647</NlmUniqueID><ISSNLinking>1535-9476</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Clin Endocrinol Metab. 2001 Feb;86(2):594-600</RefSource><PMID 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[Available on 01/01/17]</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26518761</ArticleId><ArticleId IdType="pii">M115.052332</ArticleId><ArticleId IdType="doi">10.1074/mcp.M115.052332</ArticleId><ArticleId IdType="pmc">PMC4762522</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li><li>Île-de-France</li></region><settlement><li>Maisons Alfort</li><li>Nouzilly</li></settlement></list><tree><country name="France"><region name="Centre-Val de Loire"><name sortKey="Soleilhavoup, Clement" sort="Soleilhavoup, Clement" uniqKey="Soleilhavoup C" first="Clement" last="Soleilhavoup">Clement Soleilhavoup</name></region><name sortKey="Druart, Xavier" sort="Druart, Xavier" uniqKey="Druart X" first="Xavier" last="Druart">Xavier Druart</name><name sortKey="Gerard, Nadine" sort="Gerard, Nadine" uniqKey="Gerard N" first="Nadine" last="Gerard">Nadine Gerard</name><name sortKey="Harichaux, Gregoire" sort="Harichaux, Gregoire" uniqKey="Harichaux G" first="Gregoire" last="Harichaux">Gregoire Harichaux</name><name sortKey="Kohnke, Philippa" sort="Kohnke, Philippa" uniqKey="Kohnke P" first="Philippa" last="Kohnke">Philippa Kohnke</name><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valerie" last="Labas">Valerie Labas</name><name sortKey="Reynaud, Karine" sort="Reynaud, Karine" uniqKey="Reynaud K" first="Karine" last="Reynaud">Karine Reynaud</name><name sortKey="Riou, Cindy" sort="Riou, Cindy" uniqKey="Riou C" first="Cindy" last="Riou">Cindy Riou</name><name sortKey="Tsikis, Guillaume" sort="Tsikis, Guillaume" uniqKey="Tsikis G" first="Guillaume" last="Tsikis">Guillaume Tsikis</name></country><country name="Australie"><noRegion><name sortKey="De Graaf, Simon P" sort="De Graaf, Simon P" uniqKey="De Graaf S" first="Simon P" last="De Graaf">Simon P. 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