The MTL1 Pentatricopeptide Repeat Protein Is Required for Both Translation and Splicing of the Mitochondrial NADH DEHYDROGENASE SUBUNIT7 mRNA in Arabidopsis.
Identifieur interne : 001560 ( PubMed/Checkpoint ); précédent : 001559; suivant : 001561The MTL1 Pentatricopeptide Repeat Protein Is Required for Both Translation and Splicing of the Mitochondrial NADH DEHYDROGENASE SUBUNIT7 mRNA in Arabidopsis.
Auteurs : Nawel Haïli ; Noelya Planchard ; Nadège Arnal ; Martine Quadrado ; Nathalie Vrielynck ; Jennifer Dahan ; Catherine Colas Des Francs-Small ; Hakim Mireau [France]Source :
- Plant physiology [ 1532-2548 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Arabidopsis (croissance et développement), Arabidopsis (génétique), Arabidopsis (métabolisme), Biosynthèse des protéines, Introns, Mitochondries (génétique), Mitochondries (métabolisme), Mutation, NADH dehydrogenase (génétique), NADH dehydrogenase (métabolisme), Polyribosomes (génétique), Polyribosomes (métabolisme), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés, Épissage des ARN.
- MESH :
- croissance et développement : Arabidopsis.
- génétique : ARN messager, Arabidopsis, Mitochondries, NADH dehydrogenase, Polyribosomes, Protéines d'Arabidopsis.
- métabolisme : Arabidopsis, Mitochondries, NADH dehydrogenase, Polyribosomes, Protéines d'Arabidopsis.
- Biosynthèse des protéines, Introns, Mutation, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés, Épissage des ARN.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis (metabolism), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Gene Expression Regulation, Plant, Introns, Mitochondria (genetics), Mitochondria (metabolism), Mutation, NADH Dehydrogenase (genetics), NADH Dehydrogenase (metabolism), Plants, Genetically Modified, Polyribosomes (genetics), Polyribosomes (metabolism), Protein Biosynthesis, RNA Splicing, RNA, Messenger (genetics).
- MESH :
- chemical , genetics : Arabidopsis Proteins, NADH Dehydrogenase, RNA, Messenger.
- genetics : Arabidopsis, Mitochondria, Polyribosomes.
- growth & development : Arabidopsis.
- metabolism : Arabidopsis, Arabidopsis Proteins, Mitochondria, NADH Dehydrogenase, Polyribosomes.
- Gene Expression Regulation, Plant, Introns, Mutation, Plants, Genetically Modified, Protein Biosynthesis, RNA Splicing.
Abstract
Mitochondrial translation involves a complex interplay of ancient bacteria-like features and host-derived functionalities. Although the basic components of the mitochondrial translation apparatus have been recognized, very few protein factors aiding in recruiting ribosomes on mitochondria-encoded messenger RNA (mRNAs) have been identified in higher plants. In this study, we describe the identification of the Arabidopsis (Arabidopsis thaliana) MITOCHONDRIAL TRANSLATION FACTOR1 (MTL1) protein, a new member of the Pentatricopeptide Repeat family, and show that it is essential for the translation of the mitochondrial NADH dehydrogenase subunit7 (nad7) mRNA. We demonstrate that mtl1 mutant plants fail to accumulate the Nad7 protein, even though the nad7 mature mRNA is produced and bears the same 5' and 3' extremities as in wild-type plants. We next observed that polysome association of nad7 mature mRNA is specifically disrupted in mtl1 mutants, indicating that the absence of Nad7 results from a lack of translation of nad7 mRNA. These findings illustrate that mitochondrial translation requires the intervention of gene-specific nucleus-encoded PPR trans-factors and that their action does not necessarily involve the 5' processing of their target mRNA, as observed previously. Interestingly, a partial decrease in nad7 intron 2 splicing was also detected in mtl1 mutants, suggesting that MTL1 is also involved in group II intron splicing. However, this second function appears to be less essential for nad7 expression than its role in translation. MTL1 will be instrumental to understand the multifunctionality of PPR proteins and the mechanisms governing mRNA translation and intron splicing in plant mitochondria.
DOI: 10.1104/pp.15.01591
PubMed: 26537562
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The MTL1 Pentatricopeptide Repeat Protein Is Required for Both Translation and Splicing of the Mitochondrial NADH DEHYDROGENASE SUBUNIT7 mRNA in Arabidopsis.</title><author><name sortKey="Haili, Nawel" sort="Haili, Nawel" uniqKey="Haili N" first="Nawel" last="Haïli">Nawel Haïli</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Planchard, Noelya" sort="Planchard, Noelya" uniqKey="Planchard N" first="Noelya" last="Planchard">Noelya Planchard</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Arnal, Nadege" sort="Arnal, Nadege" uniqKey="Arnal N" first="Nadège" last="Arnal">Nadège Arnal</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Quadrado, Martine" sort="Quadrado, Martine" uniqKey="Quadrado M" first="Martine" last="Quadrado">Martine Quadrado</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Vrielynck, Nathalie" sort="Vrielynck, Nathalie" uniqKey="Vrielynck N" first="Nathalie" last="Vrielynck">Nathalie Vrielynck</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Dahan, Jennifer" sort="Dahan, Jennifer" uniqKey="Dahan J" first="Jennifer" last="Dahan">Jennifer Dahan</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Des Francs Small, Catherine Colas" sort="Des Francs Small, Catherine Colas" uniqKey="Des Francs Small C" first="Catherine Colas" last="Des Francs-Small">Catherine Colas Des Francs-Small</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Mireau, Hakim" sort="Mireau, Hakim" uniqKey="Mireau H" first="Hakim" last="Mireau">Hakim Mireau</name><affiliation wicri:level="1"><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.) hakim.mireau@versailles.inra.fr.</nlm:affiliation><country wicri:rule="url">France</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26537562</idno><idno type="pmid">26537562</idno><idno type="doi">10.1104/pp.15.01591</idno><idno type="wicri:Area/PubMed/Corpus">002412</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002412</idno><idno type="wicri:Area/PubMed/Curation">002366</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002366</idno><idno type="wicri:Area/PubMed/Checkpoint">002366</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002366</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The MTL1 Pentatricopeptide Repeat Protein Is Required for Both Translation and Splicing of the Mitochondrial NADH DEHYDROGENASE SUBUNIT7 mRNA in Arabidopsis.</title><author><name sortKey="Haili, Nawel" sort="Haili, Nawel" uniqKey="Haili N" first="Nawel" last="Haïli">Nawel Haïli</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Planchard, Noelya" sort="Planchard, Noelya" uniqKey="Planchard N" first="Noelya" last="Planchard">Noelya Planchard</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Arnal, Nadege" sort="Arnal, Nadege" uniqKey="Arnal N" first="Nadège" last="Arnal">Nadège Arnal</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Quadrado, Martine" sort="Quadrado, Martine" uniqKey="Quadrado M" first="Martine" last="Quadrado">Martine Quadrado</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Vrielynck, Nathalie" sort="Vrielynck, Nathalie" uniqKey="Vrielynck N" first="Nathalie" last="Vrielynck">Nathalie Vrielynck</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Dahan, Jennifer" sort="Dahan, Jennifer" uniqKey="Dahan J" first="Jennifer" last="Dahan">Jennifer Dahan</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Des Francs Small, Catherine Colas" sort="Des Francs Small, Catherine Colas" uniqKey="Des Francs Small C" first="Catherine Colas" last="Des Francs-Small">Catherine Colas Des Francs-Small</name><affiliation><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</nlm:affiliation><wicri:noCountry code="subField">Australia (C.C.d.F.-S.).</wicri:noCountry></affiliation></author><author><name sortKey="Mireau, Hakim" sort="Mireau, Hakim" uniqKey="Mireau H" first="Hakim" last="Mireau">Hakim Mireau</name><affiliation wicri:level="1"><nlm:affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.) hakim.mireau@versailles.inra.fr.</nlm:affiliation><country wicri:rule="url">France</country></affiliation></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Arabidopsis (growth & development)</term><term>Arabidopsis (metabolism)</term><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Gene Expression Regulation, Plant</term><term>Introns</term><term>Mitochondria (genetics)</term><term>Mitochondria (metabolism)</term><term>Mutation</term><term>NADH Dehydrogenase (genetics)</term><term>NADH Dehydrogenase (metabolism)</term><term>Plants, Genetically Modified</term><term>Polyribosomes (genetics)</term><term>Polyribosomes (metabolism)</term><term>Protein Biosynthesis</term><term>RNA Splicing</term><term>RNA, Messenger (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Arabidopsis (croissance et développement)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Biosynthèse des protéines</term><term>Introns</term><term>Mitochondries (génétique)</term><term>Mitochondries (métabolisme)</term><term>Mutation</term><term>NADH dehydrogenase (génétique)</term><term>NADH dehydrogenase (métabolisme)</term><term>Polyribosomes (génétique)</term><term>Polyribosomes (métabolisme)</term><term>Protéines d'Arabidopsis (génétique)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term><term>Épissage des ARN</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term><term>NADH Dehydrogenase</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Mitochondria</term><term>Polyribosomes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Arabidopsis</term><term>Mitochondries</term><term>NADH dehydrogenase</term><term>Polyribosomes</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Arabidopsis Proteins</term><term>Mitochondria</term><term>NADH Dehydrogenase</term><term>Polyribosomes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Mitochondries</term><term>NADH dehydrogenase</term><term>Polyribosomes</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Introns</term><term>Mutation</term><term>Plants, Genetically Modified</term><term>Protein Biosynthesis</term><term>RNA Splicing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biosynthèse des protéines</term><term>Introns</term><term>Mutation</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term><term>Épissage des ARN</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mitochondrial translation involves a complex interplay of ancient bacteria-like features and host-derived functionalities. Although the basic components of the mitochondrial translation apparatus have been recognized, very few protein factors aiding in recruiting ribosomes on mitochondria-encoded messenger RNA (mRNAs) have been identified in higher plants. In this study, we describe the identification of the Arabidopsis (Arabidopsis thaliana) MITOCHONDRIAL TRANSLATION FACTOR1 (MTL1) protein, a new member of the Pentatricopeptide Repeat family, and show that it is essential for the translation of the mitochondrial NADH dehydrogenase subunit7 (nad7) mRNA. We demonstrate that mtl1 mutant plants fail to accumulate the Nad7 protein, even though the nad7 mature mRNA is produced and bears the same 5' and 3' extremities as in wild-type plants. We next observed that polysome association of nad7 mature mRNA is specifically disrupted in mtl1 mutants, indicating that the absence of Nad7 results from a lack of translation of nad7 mRNA. These findings illustrate that mitochondrial translation requires the intervention of gene-specific nucleus-encoded PPR trans-factors and that their action does not necessarily involve the 5' processing of their target mRNA, as observed previously. Interestingly, a partial decrease in nad7 intron 2 splicing was also detected in mtl1 mutants, suggesting that MTL1 is also involved in group II intron splicing. However, this second function appears to be less essential for nad7 expression than its role in translation. MTL1 will be instrumental to understand the multifunctionality of PPR proteins and the mechanisms governing mRNA translation and intron splicing in plant mitochondria.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26537562</PMID><DateCreated><Year>2016</Year><Month>01</Month><Day>01</Day></DateCreated><DateCompleted><Year>2016</Year><Month>09</Month><Day>22</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>170</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol.</ISOAbbreviation></Journal><ArticleTitle>The MTL1 Pentatricopeptide Repeat Protein Is Required for Both Translation and Splicing of the Mitochondrial NADH DEHYDROGENASE SUBUNIT7 mRNA in Arabidopsis.</ArticleTitle><Pagination><MedlinePgn>354-66</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.15.01591</ELocationID><Abstract><AbstractText>Mitochondrial translation involves a complex interplay of ancient bacteria-like features and host-derived functionalities. Although the basic components of the mitochondrial translation apparatus have been recognized, very few protein factors aiding in recruiting ribosomes on mitochondria-encoded messenger RNA (mRNAs) have been identified in higher plants. In this study, we describe the identification of the Arabidopsis (Arabidopsis thaliana) MITOCHONDRIAL TRANSLATION FACTOR1 (MTL1) protein, a new member of the Pentatricopeptide Repeat family, and show that it is essential for the translation of the mitochondrial NADH dehydrogenase subunit7 (nad7) mRNA. We demonstrate that mtl1 mutant plants fail to accumulate the Nad7 protein, even though the nad7 mature mRNA is produced and bears the same 5' and 3' extremities as in wild-type plants. We next observed that polysome association of nad7 mature mRNA is specifically disrupted in mtl1 mutants, indicating that the absence of Nad7 results from a lack of translation of nad7 mRNA. These findings illustrate that mitochondrial translation requires the intervention of gene-specific nucleus-encoded PPR trans-factors and that their action does not necessarily involve the 5' processing of their target mRNA, as observed previously. Interestingly, a partial decrease in nad7 intron 2 splicing was also detected in mtl1 mutants, suggesting that MTL1 is also involved in group II intron splicing. However, this second function appears to be less essential for nad7 expression than its role in translation. MTL1 will be instrumental to understand the multifunctionality of PPR proteins and the mechanisms governing mRNA translation and intron splicing in plant mitochondria.</AbstractText><CopyrightInformation>© 2016 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Haïli</LastName><ForeName>Nawel</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Planchard</LastName><ForeName>Noelya</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arnal</LastName><ForeName>Nadège</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Quadrado</LastName><ForeName>Martine</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vrielynck</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dahan</LastName><ForeName>Jennifer</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>des Francs-Small</LastName><ForeName>Catherine Colas</ForeName><Initials>CC</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6386-5672</Identifier><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mireau</LastName><ForeName>Hakim</ForeName><Initials>H</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-2299-5139</Identifier><AffiliationInfo><Affiliation>Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, AgroParisTech, Centre National de la Recherche Scientifique, Université Paris-Saclay, 78026 Versailles cedex, France (N.H., N.P., N.A., M.Q., N.V., J.D., H.M.);Université Paris-Sud, Université Paris-Saclay, 91405 Orsay cedex, France (N.H., N.P.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (C.C.d.F.-S.) hakim.mireau@versailles.inra.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>11</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000610873">MTL1 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C029305">mitochondrial messenger RNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.99.3</RegistryNumber><NameOfSubstance UI="C560633">NAD7 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.99.3</RegistryNumber><NameOfSubstance 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007438" MajorTopicYN="N">Introns</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009245" MajorTopicYN="N">NADH Dehydrogenase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011132" MajorTopicYN="N">Polyribosomes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012326" MajorTopicYN="Y">RNA Splicing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4704600 [Available on 01/01/17]</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>11</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26537562</ArticleId><ArticleId IdType="pii">pp.15.01591</ArticleId><ArticleId IdType="doi">10.1104/pp.15.01591</ArticleId><ArticleId IdType="pmc">PMC4704600</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Arnal, Nadege" sort="Arnal, Nadege" uniqKey="Arnal N" first="Nadège" last="Arnal">Nadège Arnal</name><name sortKey="Dahan, Jennifer" sort="Dahan, Jennifer" uniqKey="Dahan J" first="Jennifer" last="Dahan">Jennifer Dahan</name><name sortKey="Des Francs Small, Catherine Colas" sort="Des Francs Small, Catherine Colas" uniqKey="Des Francs Small C" first="Catherine Colas" last="Des Francs-Small">Catherine Colas Des Francs-Small</name><name sortKey="Haili, Nawel" sort="Haili, Nawel" uniqKey="Haili N" first="Nawel" last="Haïli">Nawel Haïli</name><name sortKey="Planchard, Noelya" sort="Planchard, Noelya" uniqKey="Planchard N" first="Noelya" last="Planchard">Noelya Planchard</name><name sortKey="Quadrado, Martine" sort="Quadrado, Martine" uniqKey="Quadrado M" first="Martine" last="Quadrado">Martine Quadrado</name><name sortKey="Vrielynck, Nathalie" sort="Vrielynck, Nathalie" uniqKey="Vrielynck N" first="Nathalie" last="Vrielynck">Nathalie Vrielynck</name></noCountry><country name="France"><noRegion><name sortKey="Mireau, Hakim" sort="Mireau, Hakim" uniqKey="Mireau H" first="Hakim" last="Mireau">Hakim 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