Characterization of microRNAs from Arabidopsis galls highlights a role for miR159 in the plant response to the root-knot nematode Meloidogyne incognita.
Identifieur interne : 001025 ( PubMed/Checkpoint ); précédent : 001024; suivant : 001026Characterization of microRNAs from Arabidopsis galls highlights a role for miR159 in the plant response to the root-knot nematode Meloidogyne incognita.
Auteurs : Clémence Medina [France] ; Martine Da Rocha [France] ; Marc Magliano [France] ; Alizée Ratpopoulo [France] ; Benoît Revel [France] ; Nathalie Marteu [France] ; Virginie Magnone [France] ; Kevin Lebrigand [France] ; Javier Cabrera [Espagne] ; Marta Barcala [Espagne] ; Ana Cláudia Silva [Espagne] ; Anthony Millar [Australie] ; Carolina Escobar [Espagne] ; Pierre Abad [France] ; Bruno Favery [France] ; Stéphanie Jaubert-Possamai [France]Source :
- The New phytologist [ 1469-8137 ] ; 2017.
Abstract
Root knot nematodes (RKN) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding cells and the development of root galls. MicroRNAs (miRNAs) regulate gene expression during development and plant responses to various stresses. Disruption of post-transcriptional gene silencing in Arabidopsis ago1 or ago2 mutants decrease the infection rate of RKN suggesting a role for this mechanism in the plant-nematode interaction. By sequencing small RNAs from uninfected Arabidopsis roots and from galls 7 and 14 d post infection with Meloidogyne incognita, we identified 24 miRNAs differentially expressed in gall as putative regulators of gall development. Moreover, strong activity within galls was detected for five miRNA promoters. Analyses of nematode development in an Arabidopsis miR159abc mutant had a lower susceptibility to RKN, suggesting a role for the miR159 family in the plant response to M. incognita. Localization of mature miR159 within the giant and surrounding cells suggested a role in giant cell and gall. Finally, overexpression of miR159 in galls at 14 d post inoculation was associated with the repression of the miR159 target MYB33 which expression is restricted to the early stages of infection. Overall, these results implicate the miR159 in plant responses to RKN.
DOI: 10.1111/nph.14717
PubMed: 28906559
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France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Marteu, Nathalie" sort="Marteu, Nathalie" uniqKey="Marteu N" first="Nathalie" last="Marteu">Nathalie Marteu</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Magnone, Virginie" sort="Magnone, Virginie" uniqKey="Magnone V" first="Virginie" last="Magnone">Virginie Magnone</name><affiliation wicri:level="1"><nlm:affiliation>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis</wicri:regionArea><wicri:noRegion>Sophia Antipolis</wicri:noRegion><wicri:noRegion>Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Lebrigand, Kevin" sort="Lebrigand, Kevin" uniqKey="Lebrigand K" first="Kevin" last="Lebrigand">Kevin Lebrigand</name><affiliation wicri:level="1"><nlm:affiliation>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis</wicri:regionArea><wicri:noRegion>Sophia Antipolis</wicri:noRegion><wicri:noRegion>Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Cabrera, Javier" sort="Cabrera, Javier" uniqKey="Cabrera J" first="Javier" last="Cabrera">Javier Cabrera</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Barcala, Marta" sort="Barcala, Marta" uniqKey="Barcala M" first="Marta" last="Barcala">Marta Barcala</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Silva, Ana Claudia" sort="Silva, Ana Claudia" uniqKey="Silva A" first="Ana Cláudia" last="Silva">Ana Cláudia Silva</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Millar, Anthony" sort="Millar, Anthony" uniqKey="Millar A" first="Anthony" last="Millar">Anthony Millar</name><affiliation wicri:level="1"><nlm:affiliation>Plant Science Division, Research School of Biology, Australian National University, Canberra, 2601, ACT, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Science Division, Research School of Biology, Australian National University, Canberra, 2601, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation></author><author><name sortKey="Escobar, Carolina" sort="Escobar, Carolina" uniqKey="Escobar C" first="Carolina" last="Escobar">Carolina Escobar</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Abad, Pierre" sort="Abad, Pierre" uniqKey="Abad P" first="Pierre" last="Abad">Pierre Abad</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Favery, Bruno" sort="Favery, Bruno" uniqKey="Favery B" first="Bruno" last="Favery">Bruno Favery</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Jaubert Possamai, Stephanie" sort="Jaubert Possamai, Stephanie" uniqKey="Jaubert Possamai S" first="Stéphanie" last="Jaubert-Possamai">Stéphanie Jaubert-Possamai</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia 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uniqKey="Medina C" first="Clémence" last="Medina">Clémence Medina</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Sophia Antipolis</settlement></placeName></affiliation></author><author><name sortKey="Da Rocha, Martine" sort="Da Rocha, Martine" uniqKey="Da Rocha M" first="Martine" last="Da Rocha">Martine Da Rocha</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Sophia Antipolis</settlement></placeName></affiliation></author><author><name sortKey="Magliano, Marc" sort="Magliano, Marc" uniqKey="Magliano M" first="Marc" last="Magliano">Marc Magliano</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Sophia Antipolis</settlement></placeName></affiliation></author><author><name sortKey="Ratpopoulo, Alizee" sort="Ratpopoulo, Alizee" uniqKey="Ratpopoulo A" first="Alizée" last="Ratpopoulo">Alizée Ratpopoulo</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Sophia Antipolis</settlement></placeName></affiliation></author><author><name sortKey="Revel, Benoit" sort="Revel, Benoit" uniqKey="Revel B" first="Benoît" last="Revel">Benoît Revel</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Sophia Antipolis</settlement></placeName></affiliation></author><author><name sortKey="Marteu, Nathalie" sort="Marteu, Nathalie" uniqKey="Marteu N" first="Nathalie" last="Marteu">Nathalie Marteu</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Sophia Antipolis</settlement></placeName></affiliation></author><author><name sortKey="Magnone, Virginie" sort="Magnone, Virginie" uniqKey="Magnone V" first="Virginie" last="Magnone">Virginie Magnone</name><affiliation wicri:level="1"><nlm:affiliation>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis</wicri:regionArea><wicri:noRegion>Sophia Antipolis</wicri:noRegion><wicri:noRegion>Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Lebrigand, Kevin" sort="Lebrigand, Kevin" uniqKey="Lebrigand K" first="Kevin" last="Lebrigand">Kevin Lebrigand</name><affiliation wicri:level="1"><nlm:affiliation>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis</wicri:regionArea><wicri:noRegion>Sophia Antipolis</wicri:noRegion><wicri:noRegion>Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Cabrera, Javier" sort="Cabrera, Javier" uniqKey="Cabrera J" first="Javier" last="Cabrera">Javier Cabrera</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Barcala, Marta" sort="Barcala, Marta" uniqKey="Barcala M" first="Marta" last="Barcala">Marta Barcala</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Silva, Ana Claudia" sort="Silva, Ana Claudia" uniqKey="Silva A" first="Ana Cláudia" last="Silva">Ana Cláudia Silva</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Millar, Anthony" sort="Millar, Anthony" uniqKey="Millar A" first="Anthony" last="Millar">Anthony Millar</name><affiliation wicri:level="1"><nlm:affiliation>Plant Science Division, Research School of Biology, Australian National University, Canberra, 2601, ACT, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Science Division, Research School of Biology, Australian National University, Canberra, 2601, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation></author><author><name sortKey="Escobar, Carolina" sort="Escobar, Carolina" uniqKey="Escobar C" first="Carolina" last="Escobar">Carolina Escobar</name><affiliation wicri:level="1"><nlm:affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo</wicri:regionArea><wicri:noRegion>Toledo</wicri:noRegion></affiliation></author><author><name sortKey="Abad, Pierre" sort="Abad, Pierre" uniqKey="Abad P" first="Pierre" last="Abad">Pierre Abad</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Favery, Bruno" sort="Favery, Bruno" uniqKey="Favery B" first="Bruno" last="Favery">Bruno Favery</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Jaubert Possamai, Stephanie" sort="Jaubert Possamai, Stephanie" uniqKey="Jaubert Possamai S" first="Stéphanie" last="Jaubert-Possamai">Stéphanie Jaubert-Possamai</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis</wicri:regionArea><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion><wicri:noRegion>06903, Sophia Antipolis</wicri:noRegion></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Root knot nematodes (RKN) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding cells and the development of root galls. MicroRNAs (miRNAs) regulate gene expression during development and plant responses to various stresses. Disruption of post-transcriptional gene silencing in Arabidopsis ago1 or ago2 mutants decrease the infection rate of RKN suggesting a role for this mechanism in the plant-nematode interaction. By sequencing small RNAs from uninfected Arabidopsis roots and from galls 7 and 14 d post infection with Meloidogyne incognita, we identified 24 miRNAs differentially expressed in gall as putative regulators of gall development. Moreover, strong activity within galls was detected for five miRNA promoters. Analyses of nematode development in an Arabidopsis miR159abc mutant had a lower susceptibility to RKN, suggesting a role for the miR159 family in the plant response to M. incognita. Localization of mature miR159 within the giant and surrounding cells suggested a role in giant cell and gall. Finally, overexpression of miR159 in galls at 14 d post inoculation was associated with the repression of the miR159 target MYB33 which expression is restricted to the early stages of infection. Overall, these results implicate the miR159 in plant responses to RKN.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">28906559</PMID><DateCreated><Year>2017</Year><Month>09</Month><Day>14</Day></DateCreated><DateRevised><Year>2017</Year><Month>10</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>216</Volume><Issue>3</Issue><PubDate><Year>2017</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol.</ISOAbbreviation></Journal><ArticleTitle>Characterization of microRNAs from Arabidopsis galls highlights a role for miR159 in the plant response to the root-knot nematode Meloidogyne incognita.</ArticleTitle><Pagination><MedlinePgn>882-896</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.14717</ELocationID><Abstract><AbstractText>Root knot nematodes (RKN) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding cells and the development of root galls. MicroRNAs (miRNAs) regulate gene expression during development and plant responses to various stresses. Disruption of post-transcriptional gene silencing in Arabidopsis ago1 or ago2 mutants decrease the infection rate of RKN suggesting a role for this mechanism in the plant-nematode interaction. By sequencing small RNAs from uninfected Arabidopsis roots and from galls 7 and 14 d post infection with Meloidogyne incognita, we identified 24 miRNAs differentially expressed in gall as putative regulators of gall development. Moreover, strong activity within galls was detected for five miRNA promoters. Analyses of nematode development in an Arabidopsis miR159abc mutant had a lower susceptibility to RKN, suggesting a role for the miR159 family in the plant response to M. incognita. Localization of mature miR159 within the giant and surrounding cells suggested a role in giant cell and gall. Finally, overexpression of miR159 in galls at 14 d post inoculation was associated with the repression of the miR159 target MYB33 which expression is restricted to the early stages of infection. Overall, these results implicate the miR159 in plant responses to RKN.</AbstractText><CopyrightInformation>© 2017 INRA. New Phytologist © 2017 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Medina</LastName><ForeName>Clémence</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Rocha</LastName><ForeName>Martine</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Magliano</LastName><ForeName>Marc</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ratpopoulo</LastName><ForeName>Alizée</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Revel</LastName><ForeName>Benoît</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marteu</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Magnone</LastName><ForeName>Virginie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lebrigand</LastName><ForeName>Kevin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>UCA Genomix, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cabrera</LastName><ForeName>Javier</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barcala</LastName><ForeName>Marta</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Ana Cláudia</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Millar</LastName><ForeName>Anthony</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Plant Science Division, Research School of Biology, Australian National University, Canberra, 2601, ACT, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Escobar</LastName><ForeName>Carolina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avda. Carlos III S/N, Edificio Sabatini, E-45071, Toledo, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abad</LastName><ForeName>Pierre</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Favery</LastName><ForeName>Bruno</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaubert-Possamai</LastName><ForeName>Stéphanie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>INRA, Université Côte d'Azur, CNRS, ISA, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>GSE100498</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>09</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis</Keyword><Keyword MajorTopicYN="N">gall</Keyword><Keyword MajorTopicYN="N">giant cell</Keyword><Keyword MajorTopicYN="N">microRNA</Keyword><Keyword MajorTopicYN="N">root knot nematode</Keyword><Keyword MajorTopicYN="N">small RNA</Keyword><Keyword MajorTopicYN="N">transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>03</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>06</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28906559</ArticleId><ArticleId IdType="doi">10.1111/nph.14717</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>Espagne</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Sophia Antipolis</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Medina, Clemence" sort="Medina, Clemence" uniqKey="Medina C" first="Clémence" last="Medina">Clémence Medina</name></region><name sortKey="Abad, Pierre" sort="Abad, Pierre" uniqKey="Abad P" first="Pierre" last="Abad">Pierre Abad</name><name sortKey="Da Rocha, Martine" sort="Da Rocha, Martine" uniqKey="Da Rocha M" first="Martine" last="Da Rocha">Martine Da Rocha</name><name sortKey="Favery, Bruno" sort="Favery, Bruno" uniqKey="Favery B" first="Bruno" last="Favery">Bruno Favery</name><name sortKey="Jaubert Possamai, Stephanie" sort="Jaubert Possamai, Stephanie" uniqKey="Jaubert Possamai S" first="Stéphanie" last="Jaubert-Possamai">Stéphanie Jaubert-Possamai</name><name sortKey="Lebrigand, Kevin" sort="Lebrigand, Kevin" uniqKey="Lebrigand K" first="Kevin" last="Lebrigand">Kevin Lebrigand</name><name sortKey="Magliano, Marc" sort="Magliano, Marc" uniqKey="Magliano M" first="Marc" last="Magliano">Marc Magliano</name><name sortKey="Magnone, Virginie" sort="Magnone, Virginie" uniqKey="Magnone V" first="Virginie" last="Magnone">Virginie Magnone</name><name sortKey="Marteu, Nathalie" sort="Marteu, Nathalie" uniqKey="Marteu N" first="Nathalie" last="Marteu">Nathalie Marteu</name><name sortKey="Ratpopoulo, Alizee" sort="Ratpopoulo, Alizee" uniqKey="Ratpopoulo A" first="Alizée" last="Ratpopoulo">Alizée Ratpopoulo</name><name sortKey="Revel, Benoit" sort="Revel, Benoit" uniqKey="Revel B" first="Benoît" last="Revel">Benoît Revel</name></country><country name="Espagne"><noRegion><name sortKey="Cabrera, Javier" sort="Cabrera, Javier" uniqKey="Cabrera J" first="Javier" last="Cabrera">Javier Cabrera</name></noRegion><name sortKey="Barcala, Marta" sort="Barcala, Marta" uniqKey="Barcala M" first="Marta" last="Barcala">Marta Barcala</name><name sortKey="Escobar, Carolina" sort="Escobar, Carolina" uniqKey="Escobar C" first="Carolina" last="Escobar">Carolina Escobar</name><name sortKey="Silva, Ana Claudia" sort="Silva, Ana Claudia" uniqKey="Silva A" first="Ana Cláudia" last="Silva">Ana Cláudia Silva</name></country><country name="Australie"><noRegion><name sortKey="Millar, Anthony" sort="Millar, Anthony" uniqKey="Millar A" first="Anthony" last="Millar">Anthony Millar</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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