Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell
Identifieur interne : 002B46 ( Pmc/Curation ); précédent : 002B45; suivant : 002B47Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell
Auteurs : Benjamin Marie [France] ; Arul Marie [France] ; Daniel J. Jackson [Allemagne] ; Lionel Dubost [France] ; Bernard M. Degnan [Australie] ; Christian Milet [France] ; Frédéric Marin [France]Source :
- Proteome Science [ 1477-5956 ] ; 2010.
Abstract
The formation of the molluscan shell is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell forming tissue, the mantle. This so called "calcifying matrix" is a complex mixture of proteins and glycoproteins that is assembled and occluded within the mineral phase during the calcification process. While the importance of the calcifying matrix to shell formation has long been appreciated, most of its protein components remain uncharacterised.
Recent expressed sequence tag (EST) investigations of the mantle tissue from the tropical abalone (
This is one of the first comprehensive proteomic study of a molluscan shell, and should provide a platform for further characterization of matrix protein functions and interactions.
Url:
DOI: 10.1186/1477-5956-8-54
PubMed: 21050442
PubMed Central: 2989941
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002C96
Links to Exploration step
PMC:2989941Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteomic analysis of the organic matrix of the abalone <italic>Haliotis asinina </italic>
calcified shell</title>
<author><name sortKey="Marie, Benjamin" sort="Marie, Benjamin" uniqKey="Marie B" first="Benjamin" last="Marie">Benjamin Marie</name>
<affiliation wicri:level="1"><nlm:aff id="I1">UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marie, Arul" sort="Marie, Arul" uniqKey="Marie A" first="Arul" last="Marie">Arul Marie</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jackson, Daniel J" sort="Jackson, Daniel J" uniqKey="Jackson D" first="Daniel J" last="Jackson">Daniel J. Jackson</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Courant Research Center Geobiology, Georg-August-University of Göttingen, 37077 Göttingen, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Courant Research Center Geobiology, Georg-August-University of Göttingen, 37077 Göttingen</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dubost, Lionel" sort="Dubost, Lionel" uniqKey="Dubost L" first="Lionel" last="Dubost">Lionel Dubost</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Degnan, Bernard M" sort="Degnan, Bernard M" uniqKey="Degnan B" first="Bernard M" last="Degnan">Bernard M. Degnan</name>
<affiliation wicri:level="1"><nlm:aff id="I4">School of Biological Sciences, University of Queensland, 4072 Queensland, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Biological Sciences, University of Queensland, 4072 Queensland</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Milet, Christian" sort="Milet, Christian" uniqKey="Milet C" first="Christian" last="Milet">Christian Milet</name>
<affiliation wicri:level="1"><nlm:aff id="I5">UMR 7208 BOREA, M.N.H.N., 75005 Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR 7208 BOREA, M.N.H.N., 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marin, Frederic" sort="Marin, Frederic" uniqKey="Marin F" first="Frédéric" last="Marin">Frédéric Marin</name>
<affiliation wicri:level="1"><nlm:aff id="I1">UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21050442</idno>
<idno type="pmc">2989941</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2989941</idno>
<idno type="RBID">PMC:2989941</idno>
<idno type="doi">10.1186/1477-5956-8-54</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">002C96</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002C96</idno>
<idno type="wicri:Area/Pmc/Curation">002B46</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002B46</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proteomic analysis of the organic matrix of the abalone <italic>Haliotis asinina </italic>
calcified shell</title>
<author><name sortKey="Marie, Benjamin" sort="Marie, Benjamin" uniqKey="Marie B" first="Benjamin" last="Marie">Benjamin Marie</name>
<affiliation wicri:level="1"><nlm:aff id="I1">UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marie, Arul" sort="Marie, Arul" uniqKey="Marie A" first="Arul" last="Marie">Arul Marie</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jackson, Daniel J" sort="Jackson, Daniel J" uniqKey="Jackson D" first="Daniel J" last="Jackson">Daniel J. Jackson</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Courant Research Center Geobiology, Georg-August-University of Göttingen, 37077 Göttingen, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Courant Research Center Geobiology, Georg-August-University of Göttingen, 37077 Göttingen</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dubost, Lionel" sort="Dubost, Lionel" uniqKey="Dubost L" first="Lionel" last="Dubost">Lionel Dubost</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Degnan, Bernard M" sort="Degnan, Bernard M" uniqKey="Degnan B" first="Bernard M" last="Degnan">Bernard M. Degnan</name>
<affiliation wicri:level="1"><nlm:aff id="I4">School of Biological Sciences, University of Queensland, 4072 Queensland, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Biological Sciences, University of Queensland, 4072 Queensland</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Milet, Christian" sort="Milet, Christian" uniqKey="Milet C" first="Christian" last="Milet">Christian Milet</name>
<affiliation wicri:level="1"><nlm:aff id="I5">UMR 7208 BOREA, M.N.H.N., 75005 Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR 7208 BOREA, M.N.H.N., 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marin, Frederic" sort="Marin, Frederic" uniqKey="Marin F" first="Frédéric" last="Marin">Frédéric Marin</name>
<affiliation wicri:level="1"><nlm:aff id="I1">UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Proteome Science</title>
<idno type="eISSN">1477-5956</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The formation of the molluscan shell is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell forming tissue, the mantle. This so called "calcifying matrix" is a complex mixture of proteins and glycoproteins that is assembled and occluded within the mineral phase during the calcification process. While the importance of the calcifying matrix to shell formation has long been appreciated, most of its protein components remain uncharacterised.</p>
</sec>
<sec><title>Results</title>
<p>Recent expressed sequence tag (EST) investigations of the mantle tissue from the tropical abalone (<italic>Haliotis asinina</italic>
) provide an opportunity to further characterise the proteins in the shell by a proteomic approach. In this study, we have identified a total of 14 proteins from distinct calcified layers of the shell. Only two of these proteins have been previously characterised from abalone shells. Among the novel proteins are several glutamine- and methionine-rich motifs and hydrophobic glycine-, alanine- and acidic aspartate-rich domains. In addition, two of the new proteins contained Kunitz-like and WAP (whey acidic protein) protease inhibitor domains.</p>
</sec>
<sec><title>Conclusion</title>
<p>This is one of the first comprehensive proteomic study of a molluscan shell, and should provide a platform for further characterization of matrix protein functions and interactions.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Addadi, L" uniqKey="Addadi L">L Addadi</name>
</author>
<author><name sortKey="Joester, D" uniqKey="Joester D">D Joester</name>
</author>
<author><name sortKey="Nudelman, F" uniqKey="Nudelman F">F Nudelman</name>
</author>
<author><name sortKey="Weiner, S" uniqKey="Weiner S">S Weiner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shen, X" uniqKey="Shen X">X Shen</name>
</author>
<author><name sortKey="Belcher, Am" uniqKey="Belcher A">AM Belcher</name>
</author>
<author><name sortKey="Hansma, Pk" uniqKey="Hansma P">PK Hansma</name>
</author>
<author><name sortKey="Stucky, Gd" uniqKey="Stucky G">GD Stucky</name>
</author>
<author><name sortKey="Morse, De" uniqKey="Morse D">DE Morse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Weiss, Im" uniqKey="Weiss I">IM Weiss</name>
</author>
<author><name sortKey="Andre, S" uniqKey="Andre S">S André</name>
</author>
<author><name sortKey="Gabius, Hj" uniqKey="Gabius H">HJ Gabius</name>
</author>
<author><name sortKey="Fritz, M" uniqKey="Fritz M">M Fritz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Weiss, Im" uniqKey="Weiss I">IM Weiss</name>
</author>
<author><name sortKey="Gohring, W" uniqKey="Gohring W">W Göhring</name>
</author>
<author><name sortKey="Fritz, M" uniqKey="Fritz M">M Fritz</name>
</author>
<author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Michenfelder, M" uniqKey="Michenfelder M">M Michenfelder</name>
</author>
<author><name sortKey="Fu, G" uniqKey="Fu G">G Fu</name>
</author>
<author><name sortKey="Lawrence, C" uniqKey="Lawrence C">C Lawrence</name>
</author>
<author><name sortKey="Weaver, Jc" uniqKey="Weaver J">JC Weaver</name>
</author>
<author><name sortKey="Wustman, Ba" uniqKey="Wustman B">BA Wustman</name>
</author>
<author><name sortKey="Taranto, L" uniqKey="Taranto L">L Taranto</name>
</author>
<author><name sortKey="Evans, Js" uniqKey="Evans J">JS Evans</name>
</author>
<author><name sortKey="Morse, De" uniqKey="Morse D">DE Morse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Treccani, L" uniqKey="Treccani L">L Treccani</name>
</author>
<author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Heinemann, F" uniqKey="Heinemann F">F Heinemann</name>
</author>
<author><name sortKey="Fritz, M" uniqKey="Fritz M">M Fritz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Siedler, F" uniqKey="Siedler F">F Siedler</name>
</author>
<author><name sortKey="Treccani, L" uniqKey="Treccani L">L Treccani</name>
</author>
<author><name sortKey="Heinemann, F" uniqKey="Heinemann F">F Heinemann</name>
</author>
<author><name sortKey="Fritz, M" uniqKey="Fritz M">M Fritz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jackson, Dj" uniqKey="Jackson D">DJ Jackson</name>
</author>
<author><name sortKey="Mcdougall, C" uniqKey="Mcdougall C">C McDougall</name>
</author>
<author><name sortKey="Green, Km" uniqKey="Green K">KM Green</name>
</author>
<author><name sortKey="Simpson, F" uniqKey="Simpson F">F Simpson</name>
</author>
<author><name sortKey="Worheide, G" uniqKey="Worheide G">G Wörheide</name>
</author>
<author><name sortKey="Degnan, Bm" uniqKey="Degnan B">BM Degnan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jackson, Dj" uniqKey="Jackson D">DJ Jackson</name>
</author>
<author><name sortKey="Worheide, G" uniqKey="Worheide G">G Wörheide</name>
</author>
<author><name sortKey="Degnan, Bm" uniqKey="Degnan B">BM Degnan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jackson, Dj" uniqKey="Jackson D">DJ Jackson</name>
</author>
<author><name sortKey="Mcdougall, C" uniqKey="Mcdougall C">C McDougall</name>
</author>
<author><name sortKey="Woodcroft, B" uniqKey="Woodcroft B">B Woodcroft</name>
</author>
<author><name sortKey="Moase, P" uniqKey="Moase P">P Moase</name>
</author>
<author><name sortKey="Ross, Ra" uniqKey="Ross R">RA Ross</name>
</author>
<author><name sortKey="Kube, M" uniqKey="Kube M">M Kube</name>
</author>
<author><name sortKey="Reinhardrt, R" uniqKey="Reinhardrt R">R Reinhardrt</name>
</author>
<author><name sortKey="Rokhsar, Ds" uniqKey="Rokhsar D">DS Rokhsar</name>
</author>
<author><name sortKey="Montagnani, C" uniqKey="Montagnani C">C Montagnani</name>
</author>
<author><name sortKey="Joubert, C" uniqKey="Joubert C">C Joubert</name>
</author>
<author><name sortKey="Piquemal, D" uniqKey="Piquemal D">D Piquemal</name>
</author>
<author><name sortKey="Degnan, Bm" uniqKey="Degnan B">BM Degnan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marin, F" uniqKey="Marin F">F Marin</name>
</author>
<author><name sortKey="Luquet, G" uniqKey="Luquet G">G Luquet</name>
</author>
<author><name sortKey="Marie, B" uniqKey="Marie B">B Marie</name>
</author>
<author><name sortKey="Medakovic, D" uniqKey="Medakovic D">D Medakovic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marie, B" uniqKey="Marie B">B Marie</name>
</author>
<author><name sortKey="Luquet, G" uniqKey="Luquet G">G Luquet</name>
</author>
<author><name sortKey="Pais De Barros, Jp" uniqKey="Pais De Barros J">JP Pais De Barros</name>
</author>
<author><name sortKey="Guichard, N" uniqKey="Guichard N">N Guichard</name>
</author>
<author><name sortKey="Morel, S" uniqKey="Morel S">S Morel</name>
</author>
<author><name sortKey="Alcaraz, G" uniqKey="Alcaraz G">G Alcaraz</name>
</author>
<author><name sortKey="Bollache, L" uniqKey="Bollache L">L Bollache</name>
</author>
<author><name sortKey="Marin, F" uniqKey="Marin F">F Marin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Macek, B" uniqKey="Macek B">B Macek</name>
</author>
<author><name sortKey="Olsen, J" uniqKey="Olsen J">J Olsen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marie, B" uniqKey="Marie B">B Marie</name>
</author>
<author><name sortKey="Marin, F" uniqKey="Marin F">F Marin</name>
</author>
<author><name sortKey="Marie, A" uniqKey="Marie A">A Marie</name>
</author>
<author><name sortKey="Bedouet, L" uniqKey="Bedouet L">L Bédouet</name>
</author>
<author><name sortKey="Dubost, L" uniqKey="Dubost L">L Dubost</name>
</author>
<author><name sortKey="Alcaraz, G" uniqKey="Alcaraz G">G Alcaraz</name>
</author>
<author><name sortKey="Milet, C" uniqKey="Milet C">C Milet</name>
</author>
<author><name sortKey="Luquet, G" uniqKey="Luquet G">G Luquet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barton, S" uniqKey="Barton S">S Barton</name>
</author>
<author><name sortKey="Jacak, R" uniqKey="Jacak R">R Jacak</name>
</author>
<author><name sortKey="Khare, Sd" uniqKey="Khare S">SD Khare</name>
</author>
<author><name sortKey="Ding, F" uniqKey="Ding F">F Ding</name>
</author>
<author><name sortKey="Dokholyan, Nv" uniqKey="Dokholyan N">NV Dokholyan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manoli, F" uniqKey="Manoli F">F Manoli</name>
</author>
<author><name sortKey="Kanakis, J" uniqKey="Kanakis J">J Kanakis</name>
</author>
<author><name sortKey="Malkaj, P" uniqKey="Malkaj P">P Malkaj</name>
</author>
<author><name sortKey="Dalas, E" uniqKey="Dalas E">E Dalas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miyamoto, H" uniqKey="Miyamoto H">H Miyamoto</name>
</author>
<author><name sortKey="Miyashita, T" uniqKey="Miyashita T">T Miyashita</name>
</author>
<author><name sortKey="Okushima, M" uniqKey="Okushima M">M Okushima</name>
</author>
<author><name sortKey="Nakano, S" uniqKey="Nakano S">S Nakano</name>
</author>
<author><name sortKey="Morita, T" uniqKey="Morita T">T Morita</name>
</author>
<author><name sortKey="Matsushiro, A" uniqKey="Matsushiro A">A Matsushiro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sudo, S" uniqKey="Sudo S">S Sudo</name>
</author>
<author><name sortKey="Fujikawa, T" uniqKey="Fujikawa T">T Fujikawa</name>
</author>
<author><name sortKey="Nagakura, T" uniqKey="Nagakura T">T Nagakura</name>
</author>
<author><name sortKey="Ohkubo, T" uniqKey="Ohkubo T">T Ohkubo</name>
</author>
<author><name sortKey="Sakagushi, K" uniqKey="Sakagushi K">K Sakagushi</name>
</author>
<author><name sortKey="Tanaka, M" uniqKey="Tanaka M">M Tanaka</name>
</author>
<author><name sortKey="Nakashima, K" uniqKey="Nakashima K">K Nakashima</name>
</author>
<author><name sortKey="Takahashi, T" uniqKey="Takahashi T">T Takahashi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sarashina, I" uniqKey="Sarashina I">I Sarashina</name>
</author>
<author><name sortKey="Endo, K" uniqKey="Endo K">K Endo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Suzuki, M" uniqKey="Suzuki M">M Suzuki</name>
</author>
<author><name sortKey="Saruwatari, K" uniqKey="Saruwatari K">K Saruwatari</name>
</author>
<author><name sortKey="Kogure, T" uniqKey="Kogure T">T Kogure</name>
</author>
<author><name sortKey="Yamamoto, Y" uniqKey="Yamamoto Y">Y Yamamoto</name>
</author>
<author><name sortKey="Nishimura, T" uniqKey="Nishimura T">T Nishimura</name>
</author>
<author><name sortKey="Kato, T" uniqKey="Kato T">T Kato</name>
</author>
<author><name sortKey="Nagasawa, H" uniqKey="Nagasawa H">H Nagasawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Korf, I" uniqKey="Korf I">I Korf</name>
</author>
<author><name sortKey="Yandell, M" uniqKey="Yandell M">M Yandell</name>
</author>
<author><name sortKey="Bedell, J" uniqKey="Bedell J">J Bedell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bedouet, L" uniqKey="Bedouet L">L Bédouet</name>
</author>
<author><name sortKey="Duplat, D" uniqKey="Duplat D">D Duplat</name>
</author>
<author><name sortKey="Marie, A" uniqKey="Marie A">A Marie</name>
</author>
<author><name sortKey="Dubost, L" uniqKey="Dubost L">L Dubost</name>
</author>
<author><name sortKey="Berland, S" uniqKey="Berland S">S Berland</name>
</author>
<author><name sortKey="Rousseau, M" uniqKey="Rousseau M">M Rousseau</name>
</author>
<author><name sortKey="Milet, C" uniqKey="Milet C">C Milet</name>
</author>
<author><name sortKey="Lopez, E" uniqKey="Lopez E">E Lopez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Hl" uniqKey="Liu H">HL Liu</name>
</author>
<author><name sortKey="Liu, Sf" uniqKey="Liu S">SF Liu</name>
</author>
<author><name sortKey="Ge, Yj" uniqKey="Ge Y">YJ Ge</name>
</author>
<author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name>
</author>
<author><name sortKey="Wang, Xy" uniqKey="Wang X">XY Wang</name>
</author>
<author><name sortKey="Xie, Lp" uniqKey="Xie L">LP Xie</name>
</author>
<author><name sortKey="Zhang, Rq" uniqKey="Zhang R">RQ Zhang</name>
</author>
<author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Poustka, Aj" uniqKey="Poustka A">AJ Poustka</name>
</author>
<author><name sortKey="Mann, M" uniqKey="Mann M">M Mann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Poustka, Aj" uniqKey="Poustka A">AJ Poustka</name>
</author>
<author><name sortKey="Mann, M" uniqKey="Mann M">M Mann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamakoshi, Y" uniqKey="Yamakoshi Y">Y Yamakoshi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gregorio King, Cc" uniqKey="Gregorio King C">CC Gregorio-King</name>
</author>
<author><name sortKey="Mcleod, Jl" uniqKey="Mcleod J">JL McLeod</name>
</author>
<author><name sortKey="Collier, Fm" uniqKey="Collier F">FM Collier</name>
</author>
<author><name sortKey="Collier, Gr" uniqKey="Collier G">GR Collier</name>
</author>
<author><name sortKey="Bolton, Ka" uniqKey="Bolton K">KA Bolton</name>
</author>
<author><name sortKey="Van Der Meer, Gj" uniqKey="Van Der Meer G">GJ Van Der Meer</name>
</author>
<author><name sortKey="Apostolopoulos, J" uniqKey="Apostolopoulos J">J Apostolopoulos</name>
</author>
<author><name sortKey="Kirkland, Ma" uniqKey="Kirkland M">MA Kirkland</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ganss, B" uniqKey="Ganss B">B Ganss</name>
</author>
<author><name sortKey="Hoffmann, W" uniqKey="Hoffmann W">W Hoffmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shashoua, Ve" uniqKey="Shashoua V">VE Shashoua</name>
</author>
<author><name sortKey="Hesse, Gw" uniqKey="Hesse G">GW Hesse</name>
</author>
<author><name sortKey="Milinazzo, B" uniqKey="Milinazzo B">B Milinazzo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Esles, Ja" uniqKey="Esles J">JA Esles</name>
</author>
<author><name sortKey="Lindberg, Dr" uniqKey="Lindberg D">DR Lindberg</name>
</author>
<author><name sortKey="Wray, C" uniqKey="Wray C">C Wray</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takeuchi, T" uniqKey="Takeuchi T">T Takeuchi</name>
</author>
<author><name sortKey="Endo, K" uniqKey="Endo K">K Endo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miyazaki, Y" uniqKey="Miyazaki Y">Y Miyazaki</name>
</author>
<author><name sortKey="Usui, T" uniqKey="Usui T">T Usui</name>
</author>
<author><name sortKey="Kajikawa, A" uniqKey="Kajikawa A">A Kajikawa</name>
</author>
<author><name sortKey="Hishiyama, H" uniqKey="Hishiyama H">H Hishiyama</name>
</author>
<author><name sortKey="Matsuzawa, N" uniqKey="Matsuzawa N">N Matsuzawa</name>
</author>
<author><name sortKey="Nishida, T" uniqKey="Nishida T">T Nishida</name>
</author>
<author><name sortKey="Machii, A" uniqKey="Machii A">A Machii</name>
</author>
<author><name sortKey="Samata, T" uniqKey="Samata T">T Samata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miyazaki, Yn" uniqKey="Miyazaki Y">YN Miyazaki</name>
</author>
<author><name sortKey="Nishida, T" uniqKey="Nishida T">T Nishida</name>
</author>
<author><name sortKey="Aoki, H" uniqKey="Aoki H">H Aoki</name>
</author>
<author><name sortKey="Samata, T" uniqKey="Samata T">T Samata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mann, K" uniqKey="Mann K">K Mann</name>
</author>
<author><name sortKey="Wilt, F" uniqKey="Wilt F">F Wilt</name>
</author>
<author><name sortKey="Poustka, Aj" uniqKey="Poustka A">AJ Poustka</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proteome Sci</journal-id>
<journal-title-group><journal-title>Proteome Science</journal-title>
</journal-title-group>
<issn pub-type="epub">1477-5956</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21050442</article-id>
<article-id pub-id-type="pmc">2989941</article-id>
<article-id pub-id-type="publisher-id">1477-5956-8-54</article-id>
<article-id pub-id-type="doi">10.1186/1477-5956-8-54</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Proteomic analysis of the organic matrix of the abalone <italic>Haliotis asinina </italic>
calcified shell</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes" id="A1"><name><surname>Marie</surname>
<given-names>Benjamin</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>benjamin.marie@u-bourgogne.fr</email>
</contrib>
<contrib contrib-type="author" id="A2"><name><surname>Marie</surname>
<given-names>Arul</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>armarie@mnhn.fr</email>
</contrib>
<contrib contrib-type="author" id="A3"><name><surname>Jackson</surname>
<given-names>Daniel J</given-names>
</name>
<xref ref-type="aff" rid="I3">3</xref>
<email>djackso@uni-goettingen.de</email>
</contrib>
<contrib contrib-type="author" id="A4"><name><surname>Dubost</surname>
<given-names>Lionel</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>ldubost@mnhn.fr</email>
</contrib>
<contrib contrib-type="author" id="A5"><name><surname>Degnan</surname>
<given-names>Bernard M</given-names>
</name>
<xref ref-type="aff" rid="I4">4</xref>
<email>b.degnan@uq.edu.au</email>
</contrib>
<contrib contrib-type="author" id="A6"><name><surname>Milet</surname>
<given-names>Christian</given-names>
</name>
<xref ref-type="aff" rid="I5">5</xref>
<email>milet@mnhn.fr</email>
</contrib>
<contrib contrib-type="author" corresp="yes" id="A7"><name><surname>Marin</surname>
<given-names>Frédéric</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>frederic.marin@u-bourgogne.fr</email>
</contrib>
</contrib-group>
<aff id="I1"><label>1</label>
UMR 5561 CNRS, Biogéosciences, Université de Bourgogne, 21000 Dijon, France</aff>
<aff id="I2"><label>2</label>
Département RDDM, Plateforme de Spectrométrie de Masse et de Protéomique/FRE3206 CNRS, Molécules de Communication et Adaptations des Micro-organismes, M.N.H.N, 75005 Paris, France</aff>
<aff id="I3"><label>3</label>
Courant Research Center Geobiology, Georg-August-University of Göttingen, 37077 Göttingen, Germany</aff>
<aff id="I4"><label>4</label>
School of Biological Sciences, University of Queensland, 4072 Queensland, Australia</aff>
<aff id="I5"><label>5</label>
UMR 7208 BOREA, M.N.H.N., 75005 Paris, France</aff>
<pub-date pub-type="collection"><year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>4</day>
<month>11</month>
<year>2010</year>
</pub-date>
<volume>8</volume>
<fpage>54</fpage>
<lpage>54</lpage>
<history><date date-type="received"><day>23</day>
<month>8</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>4</day>
<month>11</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>Copyright ©2010 Marie et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2010</copyright-year>
<copyright-holder>Marie et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<self-uri xlink:href="http://www.proteomesci.com/content/8/1/54"></self-uri>
<abstract><sec><title>Background</title>
<p>The formation of the molluscan shell is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell forming tissue, the mantle. This so called "calcifying matrix" is a complex mixture of proteins and glycoproteins that is assembled and occluded within the mineral phase during the calcification process. While the importance of the calcifying matrix to shell formation has long been appreciated, most of its protein components remain uncharacterised.</p>
</sec>
<sec><title>Results</title>
<p>Recent expressed sequence tag (EST) investigations of the mantle tissue from the tropical abalone (<italic>Haliotis asinina</italic>
) provide an opportunity to further characterise the proteins in the shell by a proteomic approach. In this study, we have identified a total of 14 proteins from distinct calcified layers of the shell. Only two of these proteins have been previously characterised from abalone shells. Among the novel proteins are several glutamine- and methionine-rich motifs and hydrophobic glycine-, alanine- and acidic aspartate-rich domains. In addition, two of the new proteins contained Kunitz-like and WAP (whey acidic protein) protease inhibitor domains.</p>
</sec>
<sec><title>Conclusion</title>
<p>This is one of the first comprehensive proteomic study of a molluscan shell, and should provide a platform for further characterization of matrix protein functions and interactions.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B46 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 002B46 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:2989941 |texte= Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:21050442" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |