Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase
Identifieur interne : 000B08 ( Pmc/Curation ); précédent : 000B07; suivant : 000B09Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase
Auteurs : Miriam Schulz-Raffelt [France, Allemagne] ; Vincent Chochois [France] ; Pascaline Auroy [France] ; Stéphan Cuiné [France] ; Emmanuelle Billon [France] ; David Dauvillée [France] ; Yonghua Li-Beisson [France] ; Gilles Peltier [France]Source :
- Biotechnology for Biofuels [ 1754-6834 ] ; 2016.
Abstract
Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga
By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a
The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants.
The online version of this article (doi:10.1186/s13068-016-0469-2) contains supplementary material, which is available to authorized users.
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DOI: 10.1186/s13068-016-0469-2
PubMed: 26958078
PubMed Central: 4782384
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Vincent Chochois<affiliation><nlm:aff id="Aff6">Research School of Biology College of Medicine, Biology and Environment, Linneaus Building 134, The Australian National University, Canberra, ACT 2601 Australia</nlm:aff><wicri:noCountry code="subfield">ACT 2601 Australia</wicri:noCountry></affiliation>Le document en format XML
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xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5">Molecular Biotechnology and Systems Biology, TU Kaiserslautern, Paul-Ehrlich-Straße 23, 67663 Kaiserslautern, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Molecular Biotechnology and Systems Biology, TU Kaiserslautern, Paul-Ehrlich-Straße 23, 67663 Kaiserslautern</wicri:regionArea></affiliation></author><author><name sortKey="Chochois, Vincent" sort="Chochois, Vincent" uniqKey="Chochois V" first="Vincent" last="Chochois">Vincent Chochois</name><affiliation wicri:level="1"><nlm:aff id="Aff1">CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation><affiliation><nlm:aff id="Aff6">Research School of Biology College of Medicine, Biology and Environment, Linneaus Building 134, The Australian National University, Canberra, ACT 2601 Australia</nlm:aff><wicri:noCountry code="subfield">ACT 2601 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Auroy, Pascaline" sort="Auroy, Pascaline" uniqKey="Auroy P" first="Pascaline" last="Auroy">Pascaline Auroy</name><affiliation wicri:level="1"><nlm:aff id="Aff1">CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Cuine, Stephan" sort="Cuine, Stephan" uniqKey="Cuine S" first="Stéphan" last="Cuiné">Stéphan Cuiné</name><affiliation wicri:level="1"><nlm:aff id="Aff1">CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Billon, Emmanuelle" sort="Billon, Emmanuelle" uniqKey="Billon E" first="Emmanuelle" last="Billon">Emmanuelle Billon</name><affiliation wicri:level="1"><nlm:aff id="Aff1">CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillée">David Dauvillée</name><affiliation wicri:level="1"><nlm:aff id="Aff4">UMR8576, CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>UMR8576, CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq</wicri:regionArea></affiliation></author><author><name sortKey="Li Beisson, Yonghua" sort="Li Beisson, Yonghua" uniqKey="Li Beisson Y" first="Yonghua" last="Li-Beisson">Yonghua Li-Beisson</name><affiliation wicri:level="1"><nlm:aff id="Aff1">CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Peltier, Gilles" sort="Peltier, Gilles" uniqKey="Peltier G" first="Gilles" last="Peltier">Gilles Peltier</name><affiliation wicri:level="1"><nlm:aff id="Aff1">CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biotechnology for Biofuels</title><idno type="eISSN">1754-6834</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga <italic>Chlamydomonas reinhardtii</italic>.</p></sec><sec><title>Results</title><p>By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a <italic>Chlamydomonas</italic> mutant (<italic>starch degradation 1, std1</italic>) deficient for a dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK). The <italic>std1</italic> mutant accumulates higher levels of starch and oil than wild-type and maintains a higher photosynthetic activity under nitrogen starvation. Phylogenetic analysis revealed that this kinase (named DYRKP) belongs to a plant-specific subgroup of the evolutionarily conserved DYRK kinase family. Furthermore, hyper-accumulation of storage compounds occurs in <italic>std1</italic> mostly under low light in photoautotrophic condition, suggesting that the kinase normally acts under conditions of low energy status to limit reserve accumulation.</p></sec><sec><title>Conclusions</title><p>The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s13068-016-0469-2) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hu, Q" uniqKey="Hu Q">Q Hu</name></author><author><name sortKey="Sommerfeld, M" uniqKey="Sommerfeld M">M Sommerfeld</name></author><author><name sortKey="Jarvis, E" uniqKey="Jarvis E">E Jarvis</name></author><author><name sortKey="Ghirardi, M" uniqKey="Ghirardi M">M Ghirardi</name></author><author><name sortKey="Posewitz, M" uniqKey="Posewitz M">M Posewitz</name></author><author><name sortKey="Seibert, M" uniqKey="Seibert M">M Seibert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wijffels, Rh" uniqKey="Wijffels R">RH 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biotechnol Biofuels</journal-id><journal-id journal-id-type="iso-abbrev">Biotechnol Biofuels</journal-id><journal-title-group><journal-title>Biotechnology for Biofuels</journal-title></journal-title-group><issn pub-type="epub">1754-6834</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26958078</article-id><article-id pub-id-type="pmc">4782384</article-id><article-id pub-id-type="publisher-id">469</article-id><article-id pub-id-type="doi">10.1186/s13068-016-0469-2</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Hyper-accumulation of starch and oil in a <italic>Chlamydomonas</italic> mutant affected in a plant-specific DYRK kinase</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Schulz-Raffelt</surname><given-names>Miriam</given-names></name><address><email>miriam@schulz-raffelt.de</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref><xref ref-type="aff" rid="Aff5"></xref></contrib><contrib contrib-type="author"><name><surname>Chochois</surname><given-names>Vincent</given-names></name><address><email>vincent.chochois@anu.edu.au</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref><xref ref-type="aff" rid="Aff6"></xref></contrib><contrib contrib-type="author"><name><surname>Auroy</surname><given-names>Pascaline</given-names></name><address><email>pascaline.auroy@cea.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Cuiné</surname><given-names>Stéphan</given-names></name><address><email>stephan.cuine@cea.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Billon</surname><given-names>Emmanuelle</given-names></name><address><email>emmanuelle.billon@cea.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Dauvillée</surname><given-names>David</given-names></name><address><email>david.dauvillee@univ-lille1.fr</email></address><xref ref-type="aff" rid="Aff4"></xref></contrib><contrib contrib-type="author"><name><surname>Li-Beisson</surname><given-names>Yonghua</given-names></name><address><email>yonghua.li@cea.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Peltier</surname><given-names>Gilles</given-names></name><address><email>gilles.peltier@cea.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><aff id="Aff1"><label></label>CEA, CNRS, Aix-Marseille Université, Institut de Biosciences et Biotechnologies Aix Marseille, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, 13108 Saint-Paul-lez-Durance, France</aff><aff id="Aff2"><label></label>CNRS, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13108 Saint-Paul-lez-Durance, France</aff><aff id="Aff3"><label></label>Aix Marseille Université, Biologie Végétale et Microbiologie Environnementale, UMR7265, 13284 Marseille, France</aff><aff id="Aff4"><label></label>UMR8576, CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq, France</aff><aff id="Aff5"><label></label>Molecular Biotechnology and Systems Biology, TU Kaiserslautern, Paul-Ehrlich-Straße 23, 67663 Kaiserslautern, Germany</aff><aff id="Aff6"><label></label>Research School of Biology College of Medicine, Biology and Environment, Linneaus Building 134, The Australian National University, Canberra, ACT 2601 Australia</aff></contrib-group><pub-date pub-type="epub"><day>8</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>8</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>9</volume><elocation-id>55</elocation-id><history><date date-type="received"><day>4</day><month>9</month><year>2015</year></date><date date-type="accepted"><day>19</day><month>2</month><year>2016</year></date></history><permissions><copyright-statement>© Schulz‑Raffelt et al. 2016</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga <italic>Chlamydomonas reinhardtii</italic>.</p></sec><sec><title>Results</title><p>By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a <italic>Chlamydomonas</italic> mutant (<italic>starch degradation 1, std1</italic>) deficient for a dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK). The <italic>std1</italic> mutant accumulates higher levels of starch and oil than wild-type and maintains a higher photosynthetic activity under nitrogen starvation. Phylogenetic analysis revealed that this kinase (named DYRKP) belongs to a plant-specific subgroup of the evolutionarily conserved DYRK kinase family. Furthermore, hyper-accumulation of storage compounds occurs in <italic>std1</italic> mostly under low light in photoautotrophic condition, suggesting that the kinase normally acts under conditions of low energy status to limit reserve accumulation.</p></sec><sec><title>Conclusions</title><p>The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s13068-016-0469-2) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd><italic>Chlamydomonas</italic></kwd><kwd>DYRK</kwd><kwd>Kinase</kwd><kwd>Microalgae</kwd><kwd>Nutrient deprivation</kwd><kwd>Oil</kwd><kwd>Photosynthesis</kwd><kwd>Starch</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001665</institution-id><institution>Agence Nationale de la Recherche (FR)</institution></institution-wrap></funding-source><award-id>ALGOMICS</award-id><award-id>ALGO-H2</award-id><award-id>ANR-11-IDEX-001-02 Amidex</award-id><principal-award-recipient><name><surname>Peltier</surname><given-names>Gilles</given-names></name></principal-award-recipient></award-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001659</institution-id><institution>Deutsche Forschungsgemeinschaft (DE)</institution></institution-wrap></funding-source><award-id>SCHU 2877/1-1</award-id><principal-award-recipient><name><surname>Schulz-Raffelt</surname><given-names>Miriam</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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