Discovery of a tyrosine-rich sporocyst wall protein in Eimeria tenella
Identifieur interne : 000930 ( Pmc/Curation ); précédent : 000929; suivant : 000931Discovery of a tyrosine-rich sporocyst wall protein in Eimeria tenella
Auteurs : Robert A. Walker [Suisse] ; Alisson Niepceron [France] ; Chandra Ramakrishnan [Suisse] ; Laura Sedano [France] ; Adrian B. Hehl [Suisse] ; Fabien Brossier [France] ; Nicholas C. SmithSource :
- Parasites & Vectors [ 1756-3305 ] ; 2016.
Abstract
Here, we report the discovery of a novel tyrosine-rich protein,
Tyrosine-rich proteins are known to be key components of the oocyst wall and we now demonstrate, using gene and protein analyses combined with genetic manipulation, that a novel tyrosine-rich protein is specific for the sporocyst wall. This finding is important because it shows that the biochemistry of these two distinct walls is similar and, hence, brings targeted disruption of sporulation and, therefore, potential neutralisation of oocysts in the environment, a step closer.
The online version of this article (doi:10.1186/s13071-016-1410-z) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s13071-016-1410-z
PubMed: 26935317
PubMed Central: 4776368
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Robert A. Walker<affiliation><nlm:aff id="Aff1">Queensland Tropical Health Alliance Research Laboratory, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns Campus, McGregor Road, Smithfield, QLD 4878 Australia</nlm:aff>
<wicri:noCountry code="subfield">QLD 4878 Australia</wicri:noCountry>
</affiliation>
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<wicri:noCountry code="subfield">QLD 4878 Australia</wicri:noCountry>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Discovery of a tyrosine-rich sporocyst wall protein in <italic>Eimeria tenella</italic>
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<author><name sortKey="Ramakrishnan, Chandra" sort="Ramakrishnan, Chandra" uniqKey="Ramakrishnan C" first="Chandra" last="Ramakrishnan">Chandra Ramakrishnan</name>
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<author><name sortKey="Hehl, Adrian B" sort="Hehl, Adrian B" uniqKey="Hehl A" first="Adrian B." last="Hehl">Adrian B. Hehl</name>
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<author><name sortKey="Smith, Nicholas C" sort="Smith, Nicholas C" uniqKey="Smith N" first="Nicholas C." last="Smith">Nicholas C. Smith</name>
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<series><title level="j">Parasites & Vectors</title>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p><italic>Eimeria </italic>
is an important genus of apicomplexan parasites. A defining feature of these parasites is the oocyst, which is transmitted into the environment <italic>via</italic>
the faeces of definitive hosts. The oocyst wall contains cross-linked, tyrosine-rich proteins and protects eight infectious sporozoites, housed in pairs within a second walled structure, the sporocyst. The biochemical basis for sporocyst wall formation is not known.</p>
</sec>
<sec><title>Findings</title>
<p>Here, we report the discovery of a novel tyrosine-rich protein, <italic>Et</italic>
SWP1, in <italic>Eimeria tenella</italic>
. Like the tyrosine-rich proteins of the oocyst wall, <italic>Et</italic>
SWP1 is an intrinsically disordered protein with the tyrosine residues concentrated in a specific region of the protein, located immediately following the region of intrinsic disorder. We engineered <italic>E. tenella</italic>
to express mCherry-tagged <italic>Et</italic>
SWP1 and showed that the tagged protein localises specifically to sporocyst walls<italic>,</italic>
indicating that the biochemistry of sporocyst wall assembly is analagous to that of oocyst walls.</p>
</sec>
<sec><title>Conclusions</title>
<p>Tyrosine-rich proteins are known to be key components of the oocyst wall and we now demonstrate, using gene and protein analyses combined with genetic manipulation, that a novel tyrosine-rich protein is specific for the sporocyst wall. This finding is important because it shows that the biochemistry of these two distinct walls is similar and, hence, brings targeted disruption of sporulation and, therefore, potential neutralisation of oocysts in the environment, a step closer.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13071-016-1410-z) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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<journal-id journal-id-type="iso-abbrev">Parasit Vectors</journal-id>
<journal-title-group><journal-title>Parasites & Vectors</journal-title>
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<issn pub-type="epub">1756-3305</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26935317</article-id>
<article-id pub-id-type="pmc">4776368</article-id>
<article-id pub-id-type="publisher-id">1410</article-id>
<article-id pub-id-type="doi">10.1186/s13071-016-1410-z</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Short Report</subject>
</subj-group>
</article-categories>
<title-group><article-title>Discovery of a tyrosine-rich sporocyst wall protein in <italic>Eimeria tenella</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Walker</surname>
<given-names>Robert A.</given-names>
</name>
<address><email>rob_walker80@hotmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Niepceron</surname>
<given-names>Alisson</given-names>
</name>
<address><email>alisson.niepceron@tours.inra.fr</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
<xref ref-type="aff" rid="Aff4"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ramakrishnan</surname>
<given-names>Chandra</given-names>
</name>
<address><email>chandra.ramakrishnan@uzh.ch</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sedano</surname>
<given-names>Laura</given-names>
</name>
<address><email>laura.sedano@jouy.inra.fr</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
<xref ref-type="aff" rid="Aff4"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hehl</surname>
<given-names>Adrian B.</given-names>
</name>
<address><email>Adrian.Hehl@uzh.ch</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Brossier</surname>
<given-names>Fabien</given-names>
</name>
<xref ref-type="aff" rid="Aff3"></xref>
<xref ref-type="aff" rid="Aff4"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Smith</surname>
<given-names>Nicholas C.</given-names>
</name>
<address><email>nicholas.smith@jcu.edu.au</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1"><label></label>
Queensland Tropical Health Alliance Research Laboratory, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns Campus, McGregor Road, Smithfield, QLD 4878 Australia</aff>
<aff id="Aff2"><label></label>
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland</aff>
<aff id="Aff3"><label></label>
INRA, UMR1282, Infectiologie et Santé Publique, Laboratoire Apicomplexes et Immunité Mucosale, F-37380 Nouzilly, France</aff>
<aff id="Aff4"><label></label>
Université François-Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France</aff>
</contrib-group>
<pub-date pub-type="epub"><day>2</day>
<month>3</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>2</day>
<month>3</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="collection"><year>2016</year>
</pub-date>
<volume>9</volume>
<elocation-id>124</elocation-id>
<history><date date-type="received"><day>4</day>
<month>1</month>
<year>2016</year>
</date>
<date date-type="accepted"><day>24</day>
<month>2</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>© Walker et al. 2016</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p><italic>Eimeria </italic>
is an important genus of apicomplexan parasites. A defining feature of these parasites is the oocyst, which is transmitted into the environment <italic>via</italic>
the faeces of definitive hosts. The oocyst wall contains cross-linked, tyrosine-rich proteins and protects eight infectious sporozoites, housed in pairs within a second walled structure, the sporocyst. The biochemical basis for sporocyst wall formation is not known.</p>
</sec>
<sec><title>Findings</title>
<p>Here, we report the discovery of a novel tyrosine-rich protein, <italic>Et</italic>
SWP1, in <italic>Eimeria tenella</italic>
. Like the tyrosine-rich proteins of the oocyst wall, <italic>Et</italic>
SWP1 is an intrinsically disordered protein with the tyrosine residues concentrated in a specific region of the protein, located immediately following the region of intrinsic disorder. We engineered <italic>E. tenella</italic>
to express mCherry-tagged <italic>Et</italic>
SWP1 and showed that the tagged protein localises specifically to sporocyst walls<italic>,</italic>
indicating that the biochemistry of sporocyst wall assembly is analagous to that of oocyst walls.</p>
</sec>
<sec><title>Conclusions</title>
<p>Tyrosine-rich proteins are known to be key components of the oocyst wall and we now demonstrate, using gene and protein analyses combined with genetic manipulation, that a novel tyrosine-rich protein is specific for the sporocyst wall. This finding is important because it shows that the biochemistry of these two distinct walls is similar and, hence, brings targeted disruption of sporulation and, therefore, potential neutralisation of oocysts in the environment, a step closer.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13071-016-1410-z) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd><italic>Eimeria</italic>
</kwd>
<kwd>Coccidia</kwd>
<kwd>Apicomplexa</kwd>
<kwd>Oocyst</kwd>
<kwd>Sporocyst</kwd>
<kwd>Dityrosine bond</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100000923</institution-id>
<institution>Australian Research Council</institution>
</institution-wrap>
</funding-source>
<award-id>DP0664013</award-id>
<principal-award-recipient><name><surname>Smith</surname>
<given-names>Nicholas C.</given-names>
</name>
</principal-award-recipient>
</award-group>
<award-group><funding-source><institution>Bellberry Limited</institution>
</funding-source>
<award-id>Not Applicable</award-id>
<principal-award-recipient><name><surname>Smith</surname>
<given-names>Nicholas C.</given-names>
</name>
</principal-award-recipient>
</award-group>
<award-group><funding-source><institution>Australian Government Department of Innovation, Industry, Science and Research International Science Linkage</institution>
</funding-source>
<award-id>FR110089</award-id>
<principal-award-recipient><name><surname>Smith</surname>
<given-names>Nicholas C.</given-names>
</name>
</principal-award-recipient>
</award-group>
<award-group><funding-source><institution>Swiss Government Excellence Postdoctoral Scholarship</institution>
</funding-source>
<award-id>Not Applicable</award-id>
<principal-award-recipient><name><surname>Walker</surname>
<given-names>Robert A.</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2016</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
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