Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
Identifieur interne : 000867 ( Pmc/Curation ); précédent : 000866; suivant : 000868Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
Auteurs : Oleg V. Pyankov [Russie] ; Yin Xiang Setoh [Australie] ; Sergey A. Bodnev [Russie] ; Judith H. Edmonds [Australie] ; Olga G. Pyankova [Russie] ; Stepan A. Pyankov [Russie] ; Gabor Pali [Australie] ; Shane Belford [Australie] ; Louis Lu [Australie] ; Mylinh La [Australie] ; George Lovrecz [Australie] ; Valentina A. Volchkova [France] ; Keith J. Chappell [Australie] ; Daniel Watterson [Australie] ; Glenn Marsh [Australie] ; Paul R. Young [Australie] ; Alexander A. Agafonov [Russie] ; Jillann F. Farmer [États-Unis] ; Victor E. Volchkov [France] ; Andreas Suhrbier [Australie] ; Alexander A. Khromykh [Australie]Source :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Herein we describe production of purified equine IgG obtained from horses immunized with plasmid DNA followed by boosting with Kunjin replicon virus-like particles both encoding a modified Ebola glycoprotein. Administration of the equine IgG over 5 days to cynomolgus macaques infected 24 hours previously with a lethal dose of Ebola virus suppressed viral loads by more than 5 logs and protected animals from mortality. Animals generated their own Ebola glycoprotein-specific IgG responses 9–15 days after infection, with circulating virus undetectable by day 15–17. Such equine IgG may find utility as a post-exposure prophylactic for Ebola infection and provides a low cost, scalable alternative to monoclonal antibodies, with extensive human safety data and WHO-standardized international manufacturing capability available in both high and low income countries.
Url:
DOI: 10.1038/srep41537
PubMed: 28155869
PubMed Central: 5290740
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000868
Links to Exploration step
PMC:5290740Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG</title><author><name sortKey="Pyankov, Oleg V" sort="Pyankov, Oleg V" uniqKey="Pyankov O" first="Oleg V." last="Pyankov">Oleg V. Pyankov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Setoh, Yin Xiang" sort="Setoh, Yin Xiang" uniqKey="Setoh Y" first="Yin Xiang" last="Setoh">Yin Xiang Setoh</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bodnev, Sergey A" sort="Bodnev, Sergey A" uniqKey="Bodnev S" first="Sergey A." last="Bodnev">Sergey A. Bodnev</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Edmonds, Judith H" sort="Edmonds, Judith H" uniqKey="Edmonds J" first="Judith H." last="Edmonds">Judith H. Edmonds</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pyankova, Olga G" sort="Pyankova, Olga G" uniqKey="Pyankova O" first="Olga G." last="Pyankova">Olga G. Pyankova</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pyankov, Stepan A" sort="Pyankov, Stepan A" uniqKey="Pyankov S" first="Stepan A." last="Pyankov">Stepan A. Pyankov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pali, Gabor" sort="Pali, Gabor" uniqKey="Pali G" first="Gabor" last="Pali">Gabor Pali</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Belford, Shane" sort="Belford, Shane" uniqKey="Belford S" first="Shane" last="Belford">Shane Belford</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Plasvacc Pty. Ltd.</institution>, Kalbar, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Louis" sort="Lu, Louis" uniqKey="Lu L" first="Louis" last="Lu">Louis Lu</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="La, Mylinh" sort="La, Mylinh" uniqKey="La M" first="Mylinh" last="La">Mylinh La</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lovrecz, George" sort="Lovrecz, George" uniqKey="Lovrecz G" first="George" last="Lovrecz">George Lovrecz</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Volchkova, Valentina A" sort="Volchkova, Valentina A" uniqKey="Volchkova V" first="Valentina A." last="Volchkova">Valentina A. Volchkova</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1</institution>, Ecole Normale Supérieure de Lyon,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chappell, Keith J" sort="Chappell, Keith J" uniqKey="Chappell K" first="Keith J." last="Chappell">Keith J. Chappell</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Watterson, Daniel" sort="Watterson, Daniel" uniqKey="Watterson D" first="Daniel" last="Watterson">Daniel Watterson</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marsh, Glenn" sort="Marsh, Glenn" uniqKey="Marsh G" first="Glenn" last="Marsh">Glenn Marsh</name><affiliation wicri:level="1"><nlm:aff id="a6"><institution>Australian Animal Health Laboratory, CSIRO Health and Biosecurity</institution>, Geelong, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Young, Paul R" sort="Young, Paul R" uniqKey="Young P" first="Paul R." last="Young">Paul R. Young</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Agafonov, Alexander A" sort="Agafonov, Alexander A" uniqKey="Agafonov A" first="Alexander A." last="Agafonov">Alexander A. Agafonov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Farmer, Jillann F" sort="Farmer, Jillann F" uniqKey="Farmer J" first="Jillann F." last="Farmer">Jillann F. Farmer</name><affiliation wicri:level="1"><nlm:aff id="a7"><institution>United Nations Medical Service</institution>, New York, NY 10017,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Volchkov, Victor E" sort="Volchkov, Victor E" uniqKey="Volchkov V" first="Victor E." last="Volchkov">Victor E. Volchkov</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1</institution>, Ecole Normale Supérieure de Lyon,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Suhrbier, Andreas" sort="Suhrbier, Andreas" uniqKey="Suhrbier A" first="Andreas" last="Suhrbier">Andreas Suhrbier</name><affiliation wicri:level="1"><nlm:aff id="a8"><institution>QIMR Berghofer Medical Research Institute</institution>, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Khromykh, Alexander A" sort="Khromykh, Alexander A" uniqKey="Khromykh A" first="Alexander A." last="Khromykh">Alexander A. Khromykh</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28155869</idno><idno type="pmc">5290740</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290740</idno><idno type="RBID">PMC:5290740</idno><idno type="doi">10.1038/srep41537</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000868</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000868</idno><idno type="wicri:Area/Pmc/Curation">000867</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000867</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG</title><author><name sortKey="Pyankov, Oleg V" sort="Pyankov, Oleg V" uniqKey="Pyankov O" first="Oleg V." last="Pyankov">Oleg V. Pyankov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Setoh, Yin Xiang" sort="Setoh, Yin Xiang" uniqKey="Setoh Y" first="Yin Xiang" last="Setoh">Yin Xiang Setoh</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bodnev, Sergey A" sort="Bodnev, Sergey A" uniqKey="Bodnev S" first="Sergey A." last="Bodnev">Sergey A. Bodnev</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Edmonds, Judith H" sort="Edmonds, Judith H" uniqKey="Edmonds J" first="Judith H." last="Edmonds">Judith H. Edmonds</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pyankova, Olga G" sort="Pyankova, Olga G" uniqKey="Pyankova O" first="Olga G." last="Pyankova">Olga G. Pyankova</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pyankov, Stepan A" sort="Pyankov, Stepan A" uniqKey="Pyankov S" first="Stepan A." last="Pyankov">Stepan A. Pyankov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Pali, Gabor" sort="Pali, Gabor" uniqKey="Pali G" first="Gabor" last="Pali">Gabor Pali</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Belford, Shane" sort="Belford, Shane" uniqKey="Belford S" first="Shane" last="Belford">Shane Belford</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Plasvacc Pty. Ltd.</institution>, Kalbar, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Louis" sort="Lu, Louis" uniqKey="Lu L" first="Louis" last="Lu">Louis Lu</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="La, Mylinh" sort="La, Mylinh" uniqKey="La M" first="Mylinh" last="La">Mylinh La</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lovrecz, George" sort="Lovrecz, George" uniqKey="Lovrecz G" first="George" last="Lovrecz">George Lovrecz</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Volchkova, Valentina A" sort="Volchkova, Valentina A" uniqKey="Volchkova V" first="Valentina A." last="Volchkova">Valentina A. Volchkova</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1</institution>, Ecole Normale Supérieure de Lyon,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chappell, Keith J" sort="Chappell, Keith J" uniqKey="Chappell K" first="Keith J." last="Chappell">Keith J. Chappell</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Watterson, Daniel" sort="Watterson, Daniel" uniqKey="Watterson D" first="Daniel" last="Watterson">Daniel Watterson</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marsh, Glenn" sort="Marsh, Glenn" uniqKey="Marsh G" first="Glenn" last="Marsh">Glenn Marsh</name><affiliation wicri:level="1"><nlm:aff id="a6"><institution>Australian Animal Health Laboratory, CSIRO Health and Biosecurity</institution>, Geelong, VIC,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Young, Paul R" sort="Young, Paul R" uniqKey="Young P" first="Paul R." last="Young">Paul R. Young</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Agafonov, Alexander A" sort="Agafonov, Alexander A" uniqKey="Agafonov A" first="Alexander A." last="Agafonov">Alexander A. Agafonov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Farmer, Jillann F" sort="Farmer, Jillann F" uniqKey="Farmer J" first="Jillann F." last="Farmer">Jillann F. Farmer</name><affiliation wicri:level="1"><nlm:aff id="a7"><institution>United Nations Medical Service</institution>, New York, NY 10017,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Volchkov, Victor E" sort="Volchkov, Victor E" uniqKey="Volchkov V" first="Victor E." last="Volchkov">Victor E. Volchkov</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1</institution>, Ecole Normale Supérieure de Lyon,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Suhrbier, Andreas" sort="Suhrbier, Andreas" uniqKey="Suhrbier A" first="Andreas" last="Suhrbier">Andreas Suhrbier</name><affiliation wicri:level="1"><nlm:aff id="a8"><institution>QIMR Berghofer Medical Research Institute</institution>, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Khromykh, Alexander A" sort="Khromykh, Alexander A" uniqKey="Khromykh A" first="Alexander A." last="Khromykh">Alexander A. Khromykh</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Herein we describe production of purified equine IgG obtained from horses immunized with plasmid DNA followed by boosting with Kunjin replicon virus-like particles both encoding a modified Ebola glycoprotein. Administration of the equine IgG over 5 days to cynomolgus macaques infected 24 hours previously with a lethal dose of Ebola virus suppressed viral loads by more than 5 logs and protected animals from mortality. Animals generated their own Ebola glycoprotein-specific IgG responses 9–15 days after infection, with circulating virus undetectable by day 15–17. Such equine IgG may find utility as a post-exposure prophylactic for Ebola infection and provides a low cost, scalable alternative to monoclonal antibodies, with extensive human safety data and WHO-standardized international manufacturing capability available in both high and low income countries.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cardile, A P" uniqKey="Cardile A">A. P. Cardile</name></author><author><name sortKey="Downey, L G" uniqKey="Downey L">L. G. Downey</name></author><author><name sortKey="Wiseman, P D" uniqKey="Wiseman P">P. D. Wiseman</name></author><author><name sortKey="Warren, T K" uniqKey="Warren T">T. K. Warren</name></author><author><name sortKey="Bavari, S" uniqKey="Bavari S">S. Bavari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Flyak, A I" uniqKey="Flyak A">A. I. Flyak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, X" uniqKey="Qiu X">X. Qiu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, X" uniqKey="Qiu X">X. Qiu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D. Corti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shaughnessy, A F" uniqKey="Shaughnessy A">A. F. Shaughnessy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gust, I" uniqKey="Gust I">I. Gust</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Jahrling, P B" uniqKey="Jahrling P">P. B. Jahrling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kudoyarova Zubavichene, N M" uniqKey="Kudoyarova Zubavichene N">N. M. Kudoyarova-Zubavichene</name></author><author><name sortKey="Sergeyev, N N" uniqKey="Sergeyev N">N. N. Sergeyev</name></author><author><name sortKey="Chepurnov, A A" uniqKey="Chepurnov A">A. A. Chepurnov</name></author><author><name sortKey="Netesov, S V" uniqKey="Netesov S">S. V. Netesov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mohan, G S" uniqKey="Mohan G">G. S. Mohan</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Ye, L" uniqKey="Ye L">L. Ye</name></author><author><name sortKey="Compans, R W" uniqKey="Compans R">R. W. Compans</name></author><author><name sortKey="Yang, C" uniqKey="Yang C">C. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Volchkov, V E" uniqKey="Volchkov V">V. E. Volchkov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zheng, X" uniqKey="Zheng X">X. Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reynard, O" uniqKey="Reynard O">O. Reynard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dowall, S D" uniqKey="Dowall S">S. D. Dowall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, D C" uniqKey="Chang D">D. C. Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reynard, O" uniqKey="Reynard O">O. Reynard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyankov, O V" uniqKey="Pyankov O">O. V. Pyankov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dolnik, O" uniqKey="Dolnik O">O. Dolnik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Escudero Perez, B" uniqKey="Escudero Perez B">B. Escudero-Perez</name></author><author><name sortKey="Volchkova, V A" uniqKey="Volchkova V">V. A. Volchkova</name></author><author><name sortKey="Dolnik, O" uniqKey="Dolnik O">O. Dolnik</name></author><author><name sortKey="Lawrence, P" uniqKey="Lawrence P">P. Lawrence</name></author><author><name sortKey="Volchkov, V E" uniqKey="Volchkov V">V. E. Volchkov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sullivan, N J" uniqKey="Sullivan N">N. J. Sullivan</name></author><author><name sortKey="Sanchez, A" uniqKey="Sanchez A">A. Sanchez</name></author><author><name sortKey="Rollin, P E" uniqKey="Rollin P">P. E. Rollin</name></author><author><name sortKey="Yang, Z Y" uniqKey="Yang Z">Z. Y. Yang</name></author><author><name sortKey="Nabel, G J" uniqKey="Nabel G">G. J. Nabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, X" uniqKey="Qiu X">X. Qiu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marzi, A" uniqKey="Marzi A">A. Marzi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, X" uniqKey="Qiu X">X. Qiu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frost, M J" uniqKey="Frost M">M. J. Frost</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T. G. Ksiazek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seifert, S A" uniqKey="Seifert S">S. A. Seifert</name></author><author><name sortKey="Boyer, L V" uniqKey="Boyer L">L. V. Boyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sullivan, N J" uniqKey="Sullivan N">N. J. Sullivan</name></author><author><name sortKey="Martin, J E" uniqKey="Martin J">J. E. Martin</name></author><author><name sortKey="Graham, B S" uniqKey="Graham B">B. S. Graham</name></author><author><name sortKey="Nabel, G J" uniqKey="Nabel G">G. J. Nabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baize, S" uniqKey="Baize S">S. Baize</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, X" uniqKey="Qiu X">X. Qiu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bielory, L" uniqKey="Bielory L">L. Bielory</name></author><author><name sortKey="Gascon, P" uniqKey="Gascon P">P. Gascon</name></author><author><name sortKey="Lawley, T J" uniqKey="Lawley T">T. J. Lawley</name></author><author><name sortKey="Young, N S" uniqKey="Young N">N. S. Young</name></author><author><name sortKey="Frank, M M" uniqKey="Frank M">M. M. Frank</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karmacharya, P" uniqKey="Karmacharya P">P. Karmacharya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stock, R P" uniqKey="Stock R">R. P. Stock</name></author><author><name sortKey="Massougbodji, A" uniqKey="Massougbodji A">A. Massougbodji</name></author><author><name sortKey="Alagon, A" uniqKey="Alagon A">A. Alagon</name></author><author><name sortKey="Chippaux, J P" uniqKey="Chippaux J">J. P. Chippaux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brown, N I" uniqKey="Brown N">N. I. Brown</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kucharski, A J" uniqKey="Kucharski A">A. J. Kucharski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bower, H" uniqKey="Bower H">H. Bower</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Velasquez, G E" uniqKey="Velasquez G">G. E. Velasquez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoenen, T" uniqKey="Hoenen T">T. Hoenen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reynard, O" uniqKey="Reynard O">O. Reynard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, J E" uniqKey="Lee J">J. E. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Setoh, Y X" uniqKey="Setoh Y">Y. X. Setoh</name></author><author><name sortKey="Hobson Peters, J" uniqKey="Hobson Peters J">J. Hobson-Peters</name></author><author><name sortKey="Prow, N A" uniqKey="Prow N">N. A. Prow</name></author><author><name sortKey="Young, P R" uniqKey="Young P">P. R. Young</name></author><author><name sortKey="Hall, R A" uniqKey="Hall R">R. A. Hall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marsh, G A" uniqKey="Marsh G">G. A. Marsh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicastri, E" uniqKey="Nicastri E">E. Nicastri</name></author><author><name sortKey="Petrosillo, N" uniqKey="Petrosillo N">N. Petrosillo</name></author><author><name sortKey="Vairo, F" uniqKey="Vairo F">F. Vairo</name></author><author><name sortKey="Di Caro, A" uniqKey="Di Caro A">A. Di Caro</name></author><author><name sortKey="Ippolito, G" uniqKey="Ippolito G">G. Ippolito</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28155869</article-id><article-id pub-id-type="pmc">5290740</article-id><article-id pub-id-type="pii">srep41537</article-id><article-id pub-id-type="doi">10.1038/srep41537</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Pyankov</surname><given-names>Oleg V.</given-names></name><xref ref-type="aff" rid="a1">1</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Setoh</surname><given-names>Yin Xiang</given-names></name><xref ref-type="aff" rid="a2">2</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Bodnev</surname><given-names>Sergey A.</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Edmonds</surname><given-names>Judith H.</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Pyankova</surname><given-names>Olga G.</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Pyankov</surname><given-names>Stepan A.</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Pali</surname><given-names>Gabor</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Belford</surname><given-names>Shane</given-names></name><xref ref-type="aff" rid="a3">3</xref></contrib><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Louis</given-names></name><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>La</surname><given-names>Mylinh</given-names></name><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>Lovrecz</surname><given-names>George</given-names></name><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>Volchkova</surname><given-names>Valentina A.</given-names></name><xref ref-type="aff" rid="a5">5</xref></contrib><contrib contrib-type="author"><name><surname>Chappell</surname><given-names>Keith J.</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Watterson</surname><given-names>Daniel</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Marsh</surname><given-names>Glenn</given-names></name><xref ref-type="aff" rid="a6">6</xref></contrib><contrib contrib-type="author"><name><surname>Young</surname><given-names>Paul R.</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Agafonov</surname><given-names>Alexander A.</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Farmer</surname><given-names>Jillann F.</given-names></name><xref ref-type="aff" rid="a7">7</xref></contrib><contrib contrib-type="author"><name><surname>Volchkov</surname><given-names>Victor E.</given-names></name><xref ref-type="aff" rid="a5">5</xref></contrib><contrib contrib-type="author"><name><surname>Suhrbier</surname><given-names>Andreas</given-names></name><xref ref-type="corresp" rid="c1">a</xref><xref ref-type="aff" rid="a8">8</xref><xref ref-type="author-notes" rid="n2">†</xref></contrib><contrib contrib-type="author"><name><surname>Khromykh</surname><given-names>Alexander A.</given-names></name><xref ref-type="corresp" rid="c2">b</xref><xref ref-type="aff" rid="a2">2</xref><xref ref-type="author-notes" rid="n2">†</xref></contrib><aff id="a1"><label>1</label><institution>State Center for Virology and Biotechnology Vector</institution>, Koltsovo,<country>Russian Federation</country></aff><aff id="a2"><label>2</label><institution>Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland</institution>, St Lucia, Brisbane, QLD,<country>Australia</country></aff><aff id="a3"><label>3</label><institution>Plasvacc Pty. Ltd.</institution>, Kalbar, QLD,<country>Australia</country></aff><aff id="a4"><label>4</label><institution>Bio Medical Manufacturing, Fermentation and Protein Production Facility</institution>, CSIRO, Clayton, VIC,<country>Australia</country></aff><aff id="a5"><label>5</label><institution>Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1</institution>, Ecole Normale Supérieure de Lyon,<country>France</country></aff><aff id="a6"><label>6</label><institution>Australian Animal Health Laboratory, CSIRO Health and Biosecurity</institution>, Geelong, VIC,<country>Australia</country></aff><aff id="a7"><label>7</label><institution>United Nations Medical Service</institution>, New York, NY 10017,<country>USA</country></aff><aff id="a8"><label>8</label><institution>QIMR Berghofer Medical Research Institute</institution>, Brisbane, QLD,<country>Australia</country></aff></contrib-group><author-notes><corresp id="c1"><label>a</label><email>andreas.suhrbier@qimrberghofer.edu.au</email></corresp><corresp id="c2"><label>b</label><email>a.khromykh@uq.edu.au</email></corresp><fn id="n1"><label>*</label><p>These authors contributed equally to this work.</p></fn><fn id="n2"><label>†</label><p>These authors jointly supervised this work.</p></fn></author-notes><pub-date pub-type="epub"><day>03</day><month>02</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>7</volume><elocation-id>41537</elocation-id><history><date date-type="received"><day>28</day><month>11</month><year>2016</year></date><date date-type="accepted"><day>19</day><month>12</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2017, The Author(s)</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><pmc-comment>author-paid</pmc-comment>
<license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract><p>Herein we describe production of purified equine IgG obtained from horses immunized with plasmid DNA followed by boosting with Kunjin replicon virus-like particles both encoding a modified Ebola glycoprotein. Administration of the equine IgG over 5 days to cynomolgus macaques infected 24 hours previously with a lethal dose of Ebola virus suppressed viral loads by more than 5 logs and protected animals from mortality. Animals generated their own Ebola glycoprotein-specific IgG responses 9–15 days after infection, with circulating virus undetectable by day 15–17. Such equine IgG may find utility as a post-exposure prophylactic for Ebola infection and provides a low cost, scalable alternative to monoclonal antibodies, with extensive human safety data and WHO-standardized international manufacturing capability available in both high and low income countries.</p></abstract></article-meta></front><floats-group><fig id="f1"><label>Figure 1</label><caption><title>Production and purification of anti-EBOV GP equine IgG.</title><p>(<bold>a</bold>) Schedule for horse immunization, serum sample collection and plasmapheresis. DNA - phCMV-GP/D637L. VLP - KUN-VLP-GP/D637L. (<bold>b</bold>) Anti-EBOV antibody titers of equine serum samples determined by ELISA. Inserts show virus neutralization titers; PRNT - plaque reduction neutralizing titers. (<bold>c</bold>) Workflow of IgG purification and concentration from equine plasma collected by plasmapheresis. TFF - tangential flow filtration. (<bold>d</bold>) Analysis of purified IgG product. Polyacrylamide gel electrophoreses of purified IgG product stained with Coomassie blue (left) and analysis of purified IgG product by ELISA and virus-neutralization assays (right).</p></caption><graphic xlink:href="srep41537-f1"></graphic></fig><fig id="f2"><label>Figure 2</label><caption><title>Post-exposure treatment of EBOV-infected NHPs with purified anti-EBOV GP equine IgG.</title><p>Cynomolgus macaques (<italic>Macaca fascicularis</italic>) were infected with 1000 PFU of EBOV. The first equine IgG treatment (20 ml intravenously) was given 24 h post-infection (day 1) followed by daily treatments for 4 days (20 ml intravenously), with NHP2 and NHP3 receiving an additional treatment (5 ml intravenously) on day 13 post-infection. (<bold>a</bold>) EBOV RNA (RNAemia) levels in the Control and IgG-treated NHPs expressed in PFU equivalents per ml of serum (see Materials and Methods). Red cross in the Control shows time of death. (<bold>b</bold>) Body temperature of Control and IgG-treated NHPs. Red boxes represent ≥1.5 °C fever. (<bold>c</bold>) ALT and AST levels in Control and IgG-treated NHPs. Red boxes represent values above the normal range. (<bold>d</bold>) Analysis of anti-GP antibody responses by ELISA assays; total anti-GP antibody titers (top panels) and NHP anti-GP titers (bottom panels). Yellow boxes illustrate periods when equine GP-specific IgG, but not NHP GP-specific IgG, were detected.</p></caption><graphic xlink:href="srep41537-f2"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000867 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000867 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:5290740
|texte= Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:28155869" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |