Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes
Identifieur interne : 000362 ( Pmc/Curation ); précédent : 000361; suivant : 000363Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes
Auteurs : Adrien Jacquot [France] ; Grégory Francius [France] ; Angelina Razafitianamaharavo [France] ; Fariba Dehghani [Australie] ; Ali Tamayol [États-Unis] ; Michel Linder [France] ; Elmira Arab-Tehrany [France]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
Liposomes are currently an important part of biological, pharmaceutical, medical and nutritional research, as they are considered to be among the most effective carriers for the introduction of various types of bioactive agents into target cells.
In this work, we study the lipid organization and mechanical properties of biomembranes made of marine and plant phospholipids. Membranes based on phospholipids extracted from rapeseed and salmon are studied in the form of liposome and as supported lipid bilayer. Dioleylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) are used as references to determine the lipid organization of marine and plant phospholipid based membranes. Atomic force microscopy (AFM) imaging and force spectroscopy measurements are performed to investigate the membranes' topography at the micrometer scale and to determine their mechanical properties.
The mechanical properties of the membranes are correlated to the fatty acid composition, the morphology, the electrophoretic mobility and the membrane fluidity. Thus, soft and homogeneous mechanical properties are evidenced for salmon phospholipids membrane containing various polyunsaturated fatty acids. Besides, phase segregation in rapeseed membrane and more important mechanical properties were emphasized for this type of membranes by contrast to the marine phospholipids based membranes.
This paper provides new information on the nanomechanical and morphological properties of membrane in form of liposome by AFM. The originality of this work is to characterize the physico-chemical properties of the nanoliposome from the natural sources containing various fatty acids and polar head.
Url:
DOI: 10.1371/journal.pone.0107435
PubMed: 25238543
PubMed Central: 4169657
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000362
Links to Exploration step
PMC:4169657Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes</title><author><name sortKey="Jacquot, Adrien" sort="Jacquot, Adrien" uniqKey="Jacquot A" first="Adrien" last="Jacquot">Adrien Jacquot</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Francius, Gregory" sort="Francius, Gregory" uniqKey="Francius G" first="Grégory" last="Francius">Grégory Francius</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>CNRS, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Razafitianamaharavo, Angelina" sort="Razafitianamaharavo, Angelina" uniqKey="Razafitianamaharavo A" first="Angelina" last="Razafitianamaharavo">Angelina Razafitianamaharavo</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>CNRS, Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Dehghani, Fariba" sort="Dehghani, Fariba" uniqKey="Dehghani F" first="Fariba" last="Dehghani">Fariba Dehghani</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>School of Chemical and Biomolecular Engineering, the University of Sydney, Sydney, New South Wales, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, the University of Sydney, Sydney, New South Wales</wicri:regionArea></affiliation></author><author><name sortKey="Tamayol, Ali" sort="Tamayol, Ali" uniqKey="Tamayol A" first="Ali" last="Tamayol">Ali Tamayol</name><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Linder, Michel" sort="Linder, Michel" uniqKey="Linder M" first="Michel" last="Linder">Michel Linder</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Arab Tehrany, Elmira" sort="Arab Tehrany, Elmira" uniqKey="Arab Tehrany E" first="Elmira" last="Arab-Tehrany">Elmira Arab-Tehrany</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25238543</idno><idno type="pmc">4169657</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169657</idno><idno type="RBID">PMC:4169657</idno><idno type="doi">10.1371/journal.pone.0107435</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000362</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000362</idno><idno type="wicri:Area/Pmc/Curation">000362</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000362</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes</title><author><name sortKey="Jacquot, Adrien" sort="Jacquot, Adrien" uniqKey="Jacquot A" first="Adrien" last="Jacquot">Adrien Jacquot</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Francius, Gregory" sort="Francius, Gregory" uniqKey="Francius G" first="Grégory" last="Francius">Grégory Francius</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>CNRS, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Razafitianamaharavo, Angelina" sort="Razafitianamaharavo, Angelina" uniqKey="Razafitianamaharavo A" first="Angelina" last="Razafitianamaharavo">Angelina Razafitianamaharavo</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>CNRS, Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Dehghani, Fariba" sort="Dehghani, Fariba" uniqKey="Dehghani F" first="Fariba" last="Dehghani">Fariba Dehghani</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>School of Chemical and Biomolecular Engineering, the University of Sydney, Sydney, New South Wales, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, the University of Sydney, Sydney, New South Wales</wicri:regionArea></affiliation></author><author><name sortKey="Tamayol, Ali" sort="Tamayol, Ali" uniqKey="Tamayol A" first="Ali" last="Tamayol">Ali Tamayol</name><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts</wicri:regionArea></affiliation></author><author><name sortKey="Linder, Michel" sort="Linder, Michel" uniqKey="Linder M" first="Michel" last="Linder">Michel Linder</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Arab Tehrany, Elmira" sort="Arab Tehrany, Elmira" uniqKey="Arab Tehrany E" first="Elmira" last="Arab-Tehrany">Elmira Arab-Tehrany</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Liposomes are currently an important part of biological, pharmaceutical, medical and nutritional research, as they are considered to be among the most effective carriers for the introduction of various types of bioactive agents into target cells.</p></sec><sec><title>Scope of Review</title><p>In this work, we study the lipid organization and mechanical properties of biomembranes made of marine and plant phospholipids. Membranes based on phospholipids extracted from rapeseed and salmon are studied in the form of liposome and as supported lipid bilayer. Dioleylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) are used as references to determine the lipid organization of marine and plant phospholipid based membranes. Atomic force microscopy (AFM) imaging and force spectroscopy measurements are performed to investigate the membranes' topography at the micrometer scale and to determine their mechanical properties.</p></sec><sec><title>Major Conclusions</title><p>The mechanical properties of the membranes are correlated to the fatty acid composition, the morphology, the electrophoretic mobility and the membrane fluidity. Thus, soft and homogeneous mechanical properties are evidenced for salmon phospholipids membrane containing various polyunsaturated fatty acids. Besides, phase segregation in rapeseed membrane and more important mechanical properties were emphasized for this type of membranes by contrast to the marine phospholipids based membranes.</p></sec><sec><title>General Significance</title><p>This paper provides new information on the nanomechanical and morphological properties of membrane in form of liposome by AFM. The originality of this work is to characterize the physico-chemical properties of the nanoliposome from the natural sources containing various fatty acids and polar head.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Tehrany, Ea" uniqKey="Tehrany E">EA Tehrany</name></author><author><name sortKey="Kahn, Cjf" uniqKey="Kahn C">CJF Kahn</name></author><author><name sortKey="Baravian, C" uniqKey="Baravian C">C Baravian</name></author><author><name sortKey="Maherani, B" uniqKey="Maherani B">B Maherani</name></author><author><name sortKey="Belhaj, N" uniqKey="Belhaj N">N Belhaj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fathi, M" uniqKey="Fathi M">M Fathi</name></author><author><name sortKey="Mozafari, Mr" uniqKey="Mozafari M">MR Mozafari</name></author><author><name sortKey="Mohebbi, M" uniqKey="Mohebbi M">M Mohebbi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maherani, B" uniqKey="Maherani B">B Maherani</name></author><author><name sortKey="Arab Tehrany, E" uniqKey="Arab Tehrany E">E Arab-Tehrany</name></author><author><name sortKey="Mozafari, Mr" uniqKey="Mozafari M">MR Mozafari</name></author><author><name sortKey="Gaiani, C" uniqKey="Gaiani C">C Gaiani</name></author><author><name sortKey="Linder, M" uniqKey="Linder M">M Linder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Torchilin, Vp" uniqKey="Torchilin V">VP Torchilin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brigger, I" uniqKey="Brigger I">I Brigger</name></author><author><name sortKey="Dubernet, C" uniqKey="Dubernet C">C Dubernet</name></author><author><name sortKey="Couvreur, P" uniqKey="Couvreur P">P Couvreur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Re, F" uniqKey="Re F">F Re</name></author><author><name sortKey="Cambianica, I" uniqKey="Cambianica I">I Cambianica</name></author><author><name sortKey="Zona, C" uniqKey="Zona C">C Zona</name></author><author><name sortKey="Sesana, S" uniqKey="Sesana S">S Sesana</name></author><author><name sortKey="Gregori, M" uniqKey="Gregori M">M Gregori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morandat, S" uniqKey="Morandat S">S Morandat</name></author><author><name sortKey="Azouzi, S" uniqKey="Azouzi S">S Azouzi</name></author><author><name sortKey="Beauvais, E" uniqKey="Beauvais E">E Beauvais</name></author><author><name sortKey="Mastouri, A" uniqKey="Mastouri A">A Mastouri</name></author><author><name sortKey="El Kirat, K" uniqKey="El Kirat K">K El Kirat</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Montano, Ga" uniqKey="Montano G">GA Montano</name></author><author><name sortKey="Adams, Pg" uniqKey="Adams P">PG Adams</name></author><author><name sortKey="Xiao, Xy" uniqKey="Xiao X">XY Xiao</name></author><author><name sortKey="Goodwin, Pm" uniqKey="Goodwin P">PM Goodwin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Redondo Morata, L" uniqKey="Redondo Morata L">L Redondo-Morata</name></author><author><name sortKey="Giannotti, Mi" uniqKey="Giannotti M">MI Giannotti</name></author><author><name sortKey="Sanz, F" uniqKey="Sanz F">F Sanz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sullan, Rma" uniqKey="Sullan R">RMA Sullan</name></author><author><name sortKey="Li, Jk" uniqKey="Li J">JK Li</name></author><author><name sortKey="Zou, S" uniqKey="Zou S">S Zou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="An, Hj" uniqKey="An H">HJ An</name></author><author><name sortKey="Nussio, Mr" uniqKey="Nussio M">MR Nussio</name></author><author><name sortKey="Huson, Mg" uniqKey="Huson M">MG Huson</name></author><author><name sortKey="Voelcker, Nh" uniqKey="Voelcker N">NH Voelcker</name></author><author><name sortKey="Shapter, Jg" uniqKey="Shapter J">JG Shapter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Manyes, S" uniqKey="Garcia Manyes S">S Garcia-Manyes</name></author><author><name sortKey="Oncins, G" uniqKey="Oncins G">G Oncins</name></author><author><name sortKey="Sanz, F" uniqKey="Sanz F">F Sanz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Manyes, S" uniqKey="Garcia Manyes S">S Garcia-Manyes</name></author><author><name sortKey="Oncins, G" uniqKey="Oncins G">G Oncins</name></author><author><name sortKey="Sanz, F" uniqKey="Sanz F">F Sanz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Manyes, S" uniqKey="Garcia Manyes S">S Garcia-Manyes</name></author><author><name sortKey="Oncins, G" uniqKey="Oncins G">G Oncins</name></author><author><name sortKey="Sanz, F" uniqKey="Sanz F">F Sanz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pera, I" uniqKey="Pera I">I Pera</name></author><author><name sortKey="Stark, R" uniqKey="Stark R">R Stark</name></author><author><name sortKey="Kappl, M" uniqKey="Kappl M">M Kappl</name></author><author><name sortKey="Butt, Hj" uniqKey="Butt H">HJ Butt</name></author><author><name sortKey="Benfenati, F" uniqKey="Benfenati F">F Benfenati</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chiantia, S" uniqKey="Chiantia S">S Chiantia</name></author><author><name sortKey="Ries, J" uniqKey="Ries J">J Ries</name></author><author><name sortKey="Kahya, N" uniqKey="Kahya N">N Kahya</name></author><author><name sortKey="Schwille, P" uniqKey="Schwille P">P Schwille</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikai, A" uniqKey="Ikai A">A Ikai</name></author><author><name sortKey="Afrin, R" uniqKey="Afrin R">R Afrin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yokokawa, M" uniqKey="Yokokawa M">M Yokokawa</name></author><author><name sortKey="Takeyasu, K" uniqKey="Takeyasu K">K Takeyasu</name></author><author><name sortKey="Yoshimura, Sh" uniqKey="Yoshimura S">SH Yoshimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Linder, M" uniqKey="Linder M">M Linder</name></author><author><name sortKey="Matouba, E" uniqKey="Matouba E">E Matouba</name></author><author><name sortKey="Fanni, J" uniqKey="Fanni J">J Fanni</name></author><author><name sortKey="Parmentier, M" uniqKey="Parmentier M">M Parmentier</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Mingeot Leclercq, Mp" uniqKey="Mingeot Leclercq M">MP Mingeot-Leclercq</name></author><author><name sortKey="Deleu, M" uniqKey="Deleu M">M Deleu</name></author><author><name sortKey="Brasseur, R" uniqKey="Brasseur R">R Brasseur</name></author><author><name sortKey="Dufrene, Yf" uniqKey="Dufrene Y">YF Dufrene</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levy, R" uniqKey="Levy R">R Levy</name></author><author><name sortKey="Maaloum, M" uniqKey="Maaloum M">M Maaloum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sneddon, In" uniqKey="Sneddon I">IN Sneddon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prendergast, Fg" uniqKey="Prendergast F">FG Prendergast</name></author><author><name sortKey="Haugland, Rp" uniqKey="Haugland R">RP Haugland</name></author><author><name sortKey="Callahan, Pj" uniqKey="Callahan P">PJ Callahan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dufrene, Yf" uniqKey="Dufrene Y">YF Dufrêne</name></author><author><name sortKey="Barger, Wr" uniqKey="Barger W">WR Barger</name></author><author><name sortKey="Green, Jbd" uniqKey="Green J">JBD Green</name></author><author><name sortKey="Lee, Gu" uniqKey="Lee G">GU Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Choucair, A" uniqKey="Choucair A">A Choucair</name></author><author><name sortKey="Chakrapani, M" uniqKey="Chakrapani M">M Chakrapani</name></author><author><name sortKey="Chakravarthy, B" uniqKey="Chakravarthy B">B Chakravarthy</name></author><author><name sortKey="Katsaras, J" uniqKey="Katsaras J">J Katsaras</name></author><author><name sortKey="Johnston, Lj" uniqKey="Johnston L">LJ Johnston</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Attwood, Sj" uniqKey="Attwood S">SJ Attwood</name></author><author><name sortKey="Choi, Y" uniqKey="Choi Y">Y Choi</name></author><author><name sortKey="Leonenko, Z" uniqKey="Leonenko Z">Z Leonenko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deleu, M" uniqKey="Deleu M">M Deleu</name></author><author><name sortKey="Lorent, J" uniqKey="Lorent J">J Lorent</name></author><author><name sortKey="Lins, L" uniqKey="Lins L">L Lins</name></author><author><name sortKey="Brasseur, R" uniqKey="Brasseur R">R Brasseur</name></author><author><name sortKey="Braun, N" uniqKey="Braun N">N Braun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shimanouchi, T" uniqKey="Shimanouchi T">T Shimanouchi</name></author><author><name sortKey="Ishii, H" uniqKey="Ishii H">H Ishii</name></author><author><name sortKey="Yoshimoto, N" uniqKey="Yoshimoto N">N Yoshimoto</name></author><author><name sortKey="Umakoshi, H" uniqKey="Umakoshi H">H Umakoshi</name></author><author><name sortKey="Kuboi, R" uniqKey="Kuboi R">R Kuboi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Manyes, S" uniqKey="Garcia Manyes S">S Garcia-Manyes</name></author><author><name sortKey="Sanz, F" uniqKey="Sanz F">F Sanz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Picas, L" uniqKey="Picas L">L Picas</name></author><author><name sortKey="Milhiet, Pe" uniqKey="Milhiet P">PE Milhiet</name></author><author><name sortKey="Hernandez Borrell, J" uniqKey="Hernandez Borrell J">J Hernandez-Borrell</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25238543</article-id><article-id pub-id-type="pmc">4169657</article-id><article-id pub-id-type="publisher-id">PONE-D-14-24684</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0107435</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology and Life Sciences</subject><subj-group><subject>Biophysics</subject></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Engineering and Technology</subject><subj-group><subject>Nanotechnology</subject></subj-group></subj-group></article-categories><title-group><article-title>Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes</article-title><alt-title alt-title-type="running-head">MorPhoBio</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jacquot</surname><given-names>Adrien</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Francius</surname><given-names>Grégory</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Razafitianamaharavo</surname><given-names>Angelina</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dehghani</surname><given-names>Fariba</given-names></name><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tamayol</surname><given-names>Ali</given-names></name><xref ref-type="aff" rid="aff7"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Linder</surname><given-names>Michel</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Arab-Tehrany</surname><given-names>Elmira</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Université de Lorraine, Laboratoire Ingénierie des Biomolécules, TSA 40602, Vandoeuvre-lès-Nancy, France</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy, France</addr-line></aff><aff id="aff3"><label>3</label><addr-line>CNRS, Laboratoire de Chimie Physique et Microbiologie pour l′Environnement, UMR 7564, Villers-lès-Nancy, France</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, France</addr-line></aff><aff id="aff5"><label>5</label><addr-line>CNRS, Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandoeuvre-lès-Nancy, France</addr-line></aff><aff id="aff6"><label>6</label><addr-line>School of Chemical and Biomolecular Engineering, the University of Sydney, Sydney, New South Wales, Australia</addr-line></aff><aff id="aff7"><label>7</label><addr-line>Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Dague</surname><given-names>Etienne</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>LAAS-CNRS, France</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>elmira.arab-tehrany@univ-lorraine.fr</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interest exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: EAT ML GF AT. Performed the experiments: AJ GF. Analyzed the data: AJ GF EAT FD. Contributed reagents/materials/analysis tools: GF AR ML EAT. Wrote the paper: AJ AT FD.</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>19</day><month>9</month><year>2014</year></pub-date><volume>9</volume><issue>9</issue><elocation-id>e107435</elocation-id><history><date date-type="received"><day>15</day><month>6</month><year>2014</year></date><date date-type="accepted"><day>11</day><month>8</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Jacquot et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><sec><title>Background</title><p>Liposomes are currently an important part of biological, pharmaceutical, medical and nutritional research, as they are considered to be among the most effective carriers for the introduction of various types of bioactive agents into target cells.</p></sec><sec><title>Scope of Review</title><p>In this work, we study the lipid organization and mechanical properties of biomembranes made of marine and plant phospholipids. Membranes based on phospholipids extracted from rapeseed and salmon are studied in the form of liposome and as supported lipid bilayer. Dioleylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) are used as references to determine the lipid organization of marine and plant phospholipid based membranes. Atomic force microscopy (AFM) imaging and force spectroscopy measurements are performed to investigate the membranes' topography at the micrometer scale and to determine their mechanical properties.</p></sec><sec><title>Major Conclusions</title><p>The mechanical properties of the membranes are correlated to the fatty acid composition, the morphology, the electrophoretic mobility and the membrane fluidity. Thus, soft and homogeneous mechanical properties are evidenced for salmon phospholipids membrane containing various polyunsaturated fatty acids. Besides, phase segregation in rapeseed membrane and more important mechanical properties were emphasized for this type of membranes by contrast to the marine phospholipids based membranes.</p></sec><sec><title>General Significance</title><p>This paper provides new information on the nanomechanical and morphological properties of membrane in form of liposome by AFM. The originality of this work is to characterize the physico-chemical properties of the nanoliposome from the natural sources containing various fatty acids and polar head.</p></sec></abstract><funding-group><funding-statement>This work was supported by French Ministry of Research and Education (MESR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="11"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its supporting information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its supporting information files.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000362 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000362 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4169657
|texte= Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:25238543" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |