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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular phylogenetics of New Caledonian <italic>Diospyros</italic>
(Ebenaceae) using plastid and nuclear markers<sup><xref ref-type="fn" rid="d32e9888">☆</xref>
</sup>
</title>
<author><name sortKey="Turner, Barbara" sort="Turner, Barbara" uniqKey="Turner B" first="Barbara" last="Turner">Barbara Turner</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Munzinger, Jerome" sort="Munzinger, Jerome" uniqKey="Munzinger J" first="Jérôme" last="Munzinger">Jérôme Munzinger</name>
<affiliation><nlm:aff id="af010">IRD, UMR AMAP, TA A51/PS2, 34398 Montpellier Cedex 5, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Duangjai, Sutee" sort="Duangjai, Sutee" uniqKey="Duangjai S" first="Sutee" last="Duangjai">Sutee Duangjai</name>
<affiliation><nlm:aff id="af015">Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok, Thailand</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Temsch, Eva M" sort="Temsch, Eva M" uniqKey="Temsch E" first="Eva M." last="Temsch">Eva M. Temsch</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Stockenhuber, Reinhold" sort="Stockenhuber, Reinhold" uniqKey="Stockenhuber R" first="Reinhold" last="Stockenhuber">Reinhold Stockenhuber</name>
<affiliation><nlm:aff id="af020">Department of Evolutionary Biology and Environmental Sciences, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Barfuss, Michael H J" sort="Barfuss, Michael H J" uniqKey="Barfuss M" first="Michael H. J." last="Barfuss">Michael H. J. Barfuss</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chase, Mark W" sort="Chase, Mark W" uniqKey="Chase M" first="Mark W." last="Chase">Mark W. Chase</name>
<affiliation><nlm:aff id="af025">Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="af030">School of Plant Biology, The University of Western Australia, Crawley, WA 6009, Australia</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Samuel, Rosabelle" sort="Samuel, Rosabelle" uniqKey="Samuel R" first="Rosabelle" last="Samuel">Rosabelle Samuel</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23850609</idno>
<idno type="pmc">3913082</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913082</idno>
<idno type="RBID">PMC:3913082</idno>
<idno type="doi">10.1016/j.ympev.2013.07.002</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">002773</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002773</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular phylogenetics of New Caledonian <italic>Diospyros</italic>
(Ebenaceae) using plastid and nuclear markers<sup><xref ref-type="fn" rid="d32e9888">☆</xref>
</sup>
</title>
<author><name sortKey="Turner, Barbara" sort="Turner, Barbara" uniqKey="Turner B" first="Barbara" last="Turner">Barbara Turner</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Munzinger, Jerome" sort="Munzinger, Jerome" uniqKey="Munzinger J" first="Jérôme" last="Munzinger">Jérôme Munzinger</name>
<affiliation><nlm:aff id="af010">IRD, UMR AMAP, TA A51/PS2, 34398 Montpellier Cedex 5, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Duangjai, Sutee" sort="Duangjai, Sutee" uniqKey="Duangjai S" first="Sutee" last="Duangjai">Sutee Duangjai</name>
<affiliation><nlm:aff id="af015">Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok, Thailand</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Temsch, Eva M" sort="Temsch, Eva M" uniqKey="Temsch E" first="Eva M." last="Temsch">Eva M. Temsch</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Stockenhuber, Reinhold" sort="Stockenhuber, Reinhold" uniqKey="Stockenhuber R" first="Reinhold" last="Stockenhuber">Reinhold Stockenhuber</name>
<affiliation><nlm:aff id="af020">Department of Evolutionary Biology and Environmental Sciences, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Barfuss, Michael H J" sort="Barfuss, Michael H J" uniqKey="Barfuss M" first="Michael H. J." last="Barfuss">Michael H. J. Barfuss</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chase, Mark W" sort="Chase, Mark W" uniqKey="Chase M" first="Mark W." last="Chase">Mark W. Chase</name>
<affiliation><nlm:aff id="af025">Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="af030">School of Plant Biology, The University of Western Australia, Crawley, WA 6009, Australia</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Samuel, Rosabelle" sort="Samuel, Rosabelle" uniqKey="Samuel R" first="Rosabelle" last="Samuel">Rosabelle Samuel</name>
<affiliation><nlm:aff id="af005">Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Molecular Phylogenetics and Evolution</title>
<idno type="ISSN">1055-7903</idno>
<idno type="eISSN">1095-9513</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
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<front><div type="abstract" xml:lang="en"><fig id="f0050" position="anchor"><graphic xlink:href="fx1"></graphic>
</fig>
</div>
</front>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Mol Phylogenet Evol</journal-id>
<journal-id journal-id-type="iso-abbrev">Mol. Phylogenet. Evol</journal-id>
<journal-title-group><journal-title>Molecular Phylogenetics and Evolution</journal-title>
</journal-title-group>
<issn pub-type="ppub">1055-7903</issn>
<issn pub-type="epub">1095-9513</issn>
<publisher><publisher-name>Academic Press</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23850609</article-id>
<article-id pub-id-type="pmc">3913082</article-id>
<article-id pub-id-type="publisher-id">S1055-7903(13)00277-7</article-id>
<article-id pub-id-type="doi">10.1016/j.ympev.2013.07.002</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Molecular phylogenetics of New Caledonian <italic>Diospyros</italic>
(Ebenaceae) using plastid and nuclear markers<sup><xref ref-type="fn" rid="d32e9888">☆</xref>
</sup>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Turner</surname>
<given-names>Barbara</given-names>
</name>
<email>barbara.turner@univie.ac.at</email>
<xref rid="af005" ref-type="aff">a</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Munzinger</surname>
<given-names>Jérôme</given-names>
</name>
<xref rid="af010" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Duangjai</surname>
<given-names>Sutee</given-names>
</name>
<xref rid="af015" ref-type="aff">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Temsch</surname>
<given-names>Eva M.</given-names>
</name>
<xref rid="af005" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Stockenhuber</surname>
<given-names>Reinhold</given-names>
</name>
<xref rid="af020" ref-type="aff">d</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Barfuss</surname>
<given-names>Michael H.J.</given-names>
</name>
<xref rid="af005" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chase</surname>
<given-names>Mark W.</given-names>
</name>
<xref rid="af025" ref-type="aff">e</xref>
<xref rid="af030" ref-type="aff">f</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Samuel</surname>
<given-names>Rosabelle</given-names>
</name>
<xref rid="af005" ref-type="aff">a</xref>
</contrib>
</contrib-group>
<aff id="af005"><label>a</label>
Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University Vienna, Rennweg 14, 1030 Wien, Austria</aff>
<aff id="af010"><label>b</label>
IRD, UMR AMAP, TA A51/PS2, 34398 Montpellier Cedex 5, France</aff>
<aff id="af015"><label>c</label>
Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok, Thailand</aff>
<aff id="af020"><label>d</label>
Department of Evolutionary Biology and Environmental Sciences, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland</aff>
<aff id="af025"><label>e</label>
Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK</aff>
<aff id="af030"><label>f</label>
School of Plant Biology, The University of Western Australia, Crawley, WA 6009, Australia</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author. <email>barbara.turner@univie.ac.at</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>1</day>
<month>12</month>
<year>2013</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>12</month>
<year>2013</year>
</pub-date>
<volume>69</volume>
<issue>3</issue>
<fpage>740</fpage>
<lpage>763</lpage>
<permissions><copyright-statement>© 2013 The Authors</copyright-statement>
<copyright-year>2013</copyright-year>
<license xlink:href="https://creativecommons.org/licenses/by-nc-nd/3.0/"><license-p>Open Access under <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by-nc-nd/3.0/">CC BY-NC-ND 3.0</ext-link>
license</license-p>
</license>
</permissions>
<abstract abstract-type="graphical"><fig id="f0050" position="anchor"><graphic xlink:href="fx1"></graphic>
</fig>
</abstract>
<abstract abstract-type="author-highlights"><title>Highlights</title>
<p><list list-type="simple"><list-item id="o0030"><label>•</label>
<p>Analyses showed resolution among 1/3 of the <italic>Diospyros</italic>
species form NC clade III.</p>
</list-item>
<list-item id="o0035"><label>•</label>
<p>Some morphological distinct species could not be discriminated with the markers used.</p>
</list-item>
<list-item id="o0040"><label>•</label>
<p><italic>Diospyros</italic>
arrived relative recently (5–20 mya) in New Caledonia.</p>
</list-item>
<list-item id="o0045"><label>•</label>
<p>Chromosome counts confirmed the investigated species to be diploid.</p>
</list-item>
<list-item id="o0050"><label>•</label>
<p>Genome sizes varied nearly fourfold among species examined.</p>
</list-item>
</list>
</p>
</abstract>
<abstract><p>To clarify phylogenetic relationships among New Caledonian species of <italic>Diospyros</italic>
, sequences of four plastid markers (<italic>atpB</italic>
, <italic>rbcL</italic>
, <italic>trnK</italic>
–<italic>matK</italic>
and <italic>trnS</italic>
–<italic>trnG</italic>
) and two low-copy nuclear markers (<italic>ncpGS</italic>
and <italic>PHYA</italic>
) were analysed. New Caledonian <italic>Diospyros</italic>
species fall into three clades, two of which have only a few members (1 or 5 species); the third has 21 closely related species for which relationships among species have been mostly unresolved in a previous study. Although species of the third group (NC clade III) are morphologically distinct and largely occupy different habitats, they exhibit little molecular variability. <italic>Diospyros vieillardii</italic>
is sister to the rest of the NC clade III, followed by <italic>D. umbrosa</italic>
and <italic>D. flavocarpa</italic>
, which are sister to the rest of this clade. Species from coastal habitats of western Grande Terre (<italic>D. cherrieri</italic>
and <italic>D. veillonii</italic>
) and some found on coralline substrates (<italic>D. calciphila</italic>
and <italic>D. inexplorata</italic>
) form two well-supported subgroups. The species of NC clade III have significantly larger genomes than found in diploid species of <italic>Diospyros</italic>
from other parts of the world, but they all appear to be diploids. By applying a molecular clock, we infer that the ancestor of the NC clade III arrived in New Caledonia around 9 million years ago. The oldest species are around 7 million years old and the youngest ones probably much less than 1 million years.</p>
</abstract>
<kwd-group><title>Keywords</title>
<kwd>Endemism</kwd>
<kwd>Genome size</kwd>
<kwd>Island flora</kwd>
<kwd>Low-copy nuclear markers</kwd>
<kwd>Molecular dating</kwd>
</kwd-group>
</article-meta>
</front>
<body><sec sec-type="intro" id="s0005"><label>1</label>
<title>Introduction</title>
<p>New Caledonia is an island group located in the southwestern Pacific about 1300 km east of Australia, ranging from around 19° to 23° south with an land area of ca. 19,000 km<sup>2</sup>
. It consists of the main island Grande Terre (ca. 16,000 km<sup>2</sup>
), Iles Belep (in the north), Ile des Pins (in the south), Loyalty Islands (in the east) and several other smaller islands. The continental part of New Caledonia (mainly Grande Terre) separated from Gondwanan during late Cretaceous (ca. 80 million years ago, mya; <xref rid="b0220" ref-type="bibr">McLoughlin, 2001</xref>
). During the Palaeocene to late Eocene, this continental sliver was submerged for at least 20 million years (myr), and a thick layer of oceanic mantle accumulated (<xref rid="b0295" ref-type="bibr">Pelletier, 2006</xref>
). After Grande Terre re-emerged in the late Eocene (37 mya), this heavy-metal rich oceanic material covered most of the land. Today, around 1/3 of the main island is still covered with ultramafic substrates. Because Grande Terre was totally submerged, it is highly unlikely that lineages that were already present in this area before the split from Gondwanan could have survived locally. Current hypotheses suggest that biota present today are derived from elements/ancestors that reached New Caledonia via long distance dispersal (e.g. <xref rid="b0245 b0310 b0145" ref-type="bibr">Morat et al., 2012; Pillon, 2012; Grandcolas et al., 2008</xref>
) mainly from Australia, New Guinea and Malaysia. Hypotheses of other islands between Australia and New Caledonia having served as stepping stones or refuges for Gondwanan taxa now endemic (e.g. <italic>Amborella</italic>
) have been proposed by a few authors (<xref rid="b0190" ref-type="bibr">Ladiges and Cantrill, 2007</xref>
), but there is no consensus of when they existed or how large and numerous they might have been. The New Caledonian climate is tropical to subtropical. The main island is split by a mountain range into a humid eastern portion (2000–4000 mm precipitation per year) and a dry western part (1000 mm precipitation per year) with prevailing winds and rain coming from the south east. New Caledonia is one of the 34 biodiversity hotspots (<xref rid="b0235 b0265" ref-type="bibr">Mittermeier et al., 2004; Myers et al., 2000</xref>
), and nearly 75% of the native flora is endemic (<xref rid="b0245" ref-type="bibr">Morat et al., 2012</xref>
), which is the fourth highest for an island (<xref rid="b0200" ref-type="bibr">Lowry, 1998</xref>
). Among these endemic taxa are 98 genera and three families, Amborellaceae, Oncothecaceae and Phellinaceae (<xref rid="b0245" ref-type="bibr">Morat et al., 2012</xref>
). One of the reasons hypothesised for the high level of endemism found in New Caledonia is the ultramafic substrates, which have acted as a filter for colonising species that were already pre-adapted to this special soil (<xref rid="b0330" ref-type="bibr">Pillon et al., 2010</xref>
).</p>
<p>Ebenaceae are pantropical and belong to the order Ericales (<xref rid="b0010" ref-type="bibr">APG, 2009</xref>
); the majority of species occur in Africa (incl. Madagascar) and the Indo-Pacific region. <xref rid="b0075" ref-type="bibr">Duangjai et al. (2006)</xref>
divided Ebenaceae into two sub families, Lissocarpoideae and Ebenoideae. Lissocarpoideae are monogeneric (<italic>Lissocarpa</italic>
, 8 species in northwestern South America), and Ebenoideae include <italic>Diospyros</italic>
, <italic>Euclea</italic>
(18 species in Africa) and <italic>Royena</italic>
(17 species in Africa). This classification of Ebenaceae in two subfamilies and four genera has been also supported by palynological data (<xref rid="b0120" ref-type="bibr">Geeraerts et al., 2009</xref>
).</p>
<p>In this paper, we use the circumscription of <italic>Diospyros</italic>
as proposed by <xref rid="b0075" ref-type="bibr">Duangjai et al. (2006)</xref>
. <italic>Diospyros</italic>
is the largest genus of Ebenaceae with more than 500 species, making it also one of the largest angiosperm genera. The greatest species of diversity is in Asia and the Pacific region (∼300 species). Fruits of some species (persimmons; e.g. <italic>D. kaki</italic>
, <italic>D. lotus</italic>
and <italic>D. virginiana</italic>
) are edible, and ebony wood (e.g. <italic>D. ebenum</italic>
) is one of the most expensive timbers. Species of <italic>Diospyros</italic>
are shrubs or trees that occur in most tropical and subtropical habitats, where they are often important and characteristic elements. <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
found 11 mostly well-resolved clades within <italic>Diospyros</italic>
. In New Caledonia, there are 31 described <italic>Diospyros</italic>
species, of which all but one are endemic, and they belong to three clades (<xref rid="b0070" ref-type="bibr">Duangjai et al., 2009</xref>
; <xref rid="f0020" ref-type="fig">Fig. 4</xref>
, clades II, III and XI). The first clade (clade II) contains five species from New Caledonia that are related to Australian species of <italic>Diospyros</italic>
. The second clade (clade III) includes species from Hawai‘i, Indian Ocean islands and 24 taxa from New Caledonia, within which the species from New Caledonia form a sublcade, here termed NC clade III. Although <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
analysed more than 8000 base pairs of plastid DNA, low variability and little resolution was found among these endemic New Caledonian species. The third clade (clade XI), consisting of taxa from Asia, America, Pacific Islands and New Caledonia, includes two <italic>Diospyros</italic>
species from New Caledonia, one endemic and the other found throughout the southern Pacific. These two species are not sister species, accounting for two more colonisations of New Caledonia (i.e. four in total). Similar, multiple colonisation events are also found among other organisms in New Caledonia (e.g. <xref rid="b0255" ref-type="bibr">Murienne et al., 2005</xref>
). <italic>Diospyros</italic>
is observed in all types of New Caledonian vegetation except mangrove; the species range from sea level up to ca. 1250 m (New Caledonia’s highest point is 1628 m). There are several micro-endemics restricted to just a small area (<xref rid="b0400" ref-type="bibr">White, 1992</xref>
). Most New Caledonian <italic>Diospyros</italic>
species from clade III are morphologically clearly defined and restricted by edaphic factors and occur on just one substrate type. For example, <italic>D. labillardierei</italic>
(<xref rid="f0005" ref-type="fig">Fig. 1</xref>
D) is distinctive with its long narrow leaves and <italic>Salix</italic>
-like habit; it is a rheophyte on non-ultramafic substrates. <italic>Diospyros veillonii</italic>
(<xref rid="f0005" ref-type="fig">Fig. 1</xref>
F) is a remarkable species with coralloid inflorescence axes (unique among New Caledonian <italic>Diospyros</italic>
) and large leaves, but is known from only a single locality in dry forest on black clay soil. Other species have broader distributions and ecologies, such as <italic>D. parviflora</italic>
(<xref rid="f0005" ref-type="fig">Fig. 1</xref>
J), which grows on both ultramafic and non-ultramafic substrates and is widespread throughout Grande Terre and Balabio Island in dense humid forests as well as in more open and dry vegetation. Some species can have similar ecological requirements, but are morphologically well differentiated; for example <italic>D. vieillardii</italic>
(<xref rid="f0005" ref-type="fig">Fig. 1</xref>
A) has a calyx narrower than its prune-like fruit, whereas <italic>D. glans</italic>
(<xref rid="f0005" ref-type="fig">Fig. 1</xref>
N) has a thick calyx much wider than its fruit, but both grow in maquis vegetation and co-occur at some sites.</p>
<p>For establishing phylogenetic relationships, sequences of low-copy nuclear genes are not as often used as regions from the plastid genome, often due to methodological difficulties. Low-copy genes are present in one or few copies in the genome, and primers are often highly specific for individual groups, requiring them therefore to be newly designed for each study. On the other hand, low-copy nuclear markers are normally highly informative and as they are biparentally inherited they may also help detect recent hybridization (e.g. <xref rid="b0240" ref-type="bibr">Moody and Rieseberg, 2012</xref>
). However, in a study of Hawaiian endemics in two unrelated genera, <xref rid="b0320" ref-type="bibr">Pillon et al. (2013)</xref>
found that although two low-copy nuclear loci displayed a high level of variability, they also exhibited heterozygosity, intraspecific variation, and retention of ancient alleles; allele coalescence was older than the species under study. Nonetheless, we hoped that inclusion of low-copy nuclear genes might provide additional insight into species relationships and thus included two such loci. Phytochrome A (<italic>PHYA</italic>
) belongs to the gene family of the phytochromes, which has eight members across the seed plants (<italic>PHYA</italic>
–<italic>PHYE</italic>
in angiosperms and <italic>PHYN</italic>
–<italic>PHYP</italic>
in gymnosperms); <italic>PHYN</italic>
/<italic>PHYA</italic>
, <italic>PHYO</italic>
/<italic>PHYC</italic>
and <italic>PHYP</italic>
/<italic>PHYBDE</italic>
are orthologs, the rest being paralogs of the others (<xref rid="b0210" ref-type="bibr">Mathews et al., 2010</xref>
). Genes of this family encode photoreceptor proteins that mediate developmental responses to red and far red light. The three main paralogs (<italic>PHYA</italic>
, <italic>PHYB</italic>
and <italic>PHYC</italic>
) are different enough to be amplified with specific primers (<xref rid="b0420" ref-type="bibr">Zimmer and Wen, 2012</xref>
). Sequences of <italic>phy</italic>
genes have been used successfully across the flowering plants (e.g. <xref rid="b0210 b0275 b0035" ref-type="bibr">Mathews et al., 2010; Nie et al., 2008; Bennett and Mathews, 2006</xref>
) for phylogenetic reconstruction. The gene <italic>PHYA</italic>
used in this study consists of four exons and three introns. Glutamine synthetase (<italic>GS</italic>
), codes for a protein involved in nitrogen assimilation. There are two main types of <italic>GS</italic>
genes, cytosolic- and chloroplast-expressed. Chloroplast-expressed glutamine synthetase (<italic>ncpGS</italic>
) consists of 12 exons and 11 introns and has been shown to be a single-copy gene in plants (<xref rid="b0085" ref-type="bibr">Emshwiller and Doyle, 1999</xref>
). This combination of coding and non-coding regions has been shown to be highly informative for inferring phylogenetic trees of various groups (e.g. Oxalidaceae, <xref rid="b0085" ref-type="bibr">Emshwiller and Doyle, 1999</xref>
; <italic>Passiflora</italic>
, <xref rid="b0410" ref-type="bibr">Yockteng and Nadot, 2004</xref>
; <italic>Spiraeanthemum</italic>
, <xref rid="b0315" ref-type="bibr">Pillon et al., 2009a</xref>
; <italic>Codia</italic>
, <xref rid="b0325" ref-type="bibr">Pillon et al., 2009b</xref>
; <italic>Achillea millefolium</italic>
, <xref rid="b0165" ref-type="bibr">Guo et al., 2012</xref>
).</p>
<p>Beside phylogenetic relationships, the age of clades is of interest. In many cases, there are no fossils available for direct dating of a group of interest in a particular region, which is often the case for islands and is certainly true for New Caledonia (the few fossils recorded to date are older than the last emergence of the island and are not certain to be angiosperms; <xref rid="b0345" ref-type="bibr">Salard and Avias, 1968</xref>
). Rates of DNA divergence are generally consistent with a molecular clock (<xref rid="b0425" ref-type="bibr">Zuckerkandl and Pauling, 1965</xref>
), and therefore DNA data contain information about the relative ages of taxa. When substitution rates (e.g. <xref rid="b0350 b0005" ref-type="bibr">Silvestro et al., 2011; Alba et al., 2000</xref>
) or fossils belonging to defined clades (e.g. <xref rid="b0335 b0205" ref-type="bibr">Pirie and Doyle, 2012; Magallón, 2010</xref>
) are taken into consideration, the relative ages obtained can be transformed into absolute ages. Placement of fossils in the correct position in the phylogenetic tree is crucial for accurate interpretation (<xref rid="b0105" ref-type="bibr">Forest, 2009</xref>
). Some previous studies have has been published on the subject of the age of asterids (e.g. <xref rid="b0225 b0030 b0050" ref-type="bibr">Millán-Martínez, 2010; Bell et al., 2010; Bremer et al., 2004</xref>
) to which Ericales belong, and fossil <italic>Diospyros</italic>
are known from some localities (mainly in India and North America), but none has been found in New Caledonia. <italic>Austrodiospyros cryptostoma</italic>
(<xref rid="b0025" ref-type="bibr">Basinger and Christophel, 1985</xref>
), a fossil from Australia has many morphological similarities to <italic>D. australis</italic>
of clade II (<xref rid="b0070" ref-type="bibr">Duangjai et al., 2009</xref>
). It is thus far the only fossil belonging to a clade that includes <italic>Diospyros</italic>
species from New Caledonia. We treat <italic>A. cryptostoma</italic>
as member of clade II in this study.</p>
<p>Genome sizes vary nearly 2400-fold across angiosperms (<xref rid="b0300" ref-type="bibr">Pellicer et al., 2010</xref>
). Most variation in DNA amount is caused by different amounts of non-coding, repetitive DNA, such as pseudogenes, retrotransposons, transposons and satellite repeats (<xref rid="b0195 b0040 b0290 b0305" ref-type="bibr">Leitch, 2007; Bennett and Leitch, 2005; Parisod et al., 2009; Petrov, 2001</xref>
). Genome sizes and chromosome numbers of <italic>Diospyros</italic>
are within the range of those of other members of Ericales (<xref rid="b0045" ref-type="bibr">Bennett and Leitch, 2010</xref>
). Nuclear DNA amounts in <italic>Diospyros</italic>
range from 0.78 pg (1C-value) in diploid <italic>D. rhodocalyx</italic>
up to 4.06 pg in nonaploid <italic>D. kaki</italic>
cultivars (<xref rid="b0370" ref-type="bibr">Tamura et al., 1998</xref>
). The basic chromosome number in <italic>Diospyros</italic>
is 2<italic>n </italic>
= 2<italic>x </italic>
= 30, and most species seem to be diploid (e.g. <xref rid="b0370 b0400" ref-type="bibr">Tamura et al., 1998; White, 1992</xref>
). There are some reports of polyploid <italic>Diospyros</italic>
, mostly from cultivated species (e.g. <italic>D. rhombifolia</italic>
4<italic>x</italic>
, <italic>D. ebenum</italic>
6<italic>x</italic>
, <italic>D. kaki</italic>
6<italic>x</italic>
and 9<italic>x</italic>
, <italic>D. virginiana</italic>
6<italic>x</italic>
and 9<italic>x</italic>
; <xref rid="b0370" ref-type="bibr">Tamura et al., 1998</xref>
). <xref rid="b0400" ref-type="bibr">White (1992)</xref>
provided chromosome counts for nine New Caledonian species of <italic>Diospyros</italic>
(<italic>D. calciphila</italic>
, <italic>D. fasciculosa</italic>
, <italic>D. flavocarpa</italic>
, <italic>D. minimifolia</italic>
, <italic>D. olen</italic>
, <italic>D. parviflora</italic>
, <italic>D. umbrosa</italic>
, <italic>D. vieillardii</italic>
and <italic>D. yaouhensis</italic>
), all of which are diploid.</p>
<p><xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
found little sequence variation in the markers investigated among many species from NC clade III, which could indicate recent diversification. <xref rid="b0400" ref-type="bibr">White (1992)</xref>
, who described most the New Caledonian <italic>Diospyros</italic>
species, suspected some hybridization was taking place. The main aim of this study was to clarify relationships among New Caledonian <italic>Diospyros</italic>
species, especially of those belonging to clade III (<xref rid="b0070" ref-type="bibr">Duangjai et al., 2009</xref>
). Furthermore, if we were able to find more variable than those previously studied, we wanted to elucidate potential factors underlying speciation (e.g. ecological speciation, hybrid speciation and introgression) and understand better differences in speciation rates of the clades that reached New Caledonia independently. We used low-copy nuclear markers, <italic>PHYA</italic>
and <italic>ncpGS</italic>
because they offered the prospect of resolving relationships within this clade and detecting possible hybrid species. We also included samples from nine additional species that were not available for the study of <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
. Moreover, we conducted dating analyses to obtain estimates of the ages for the lineages to which New Caledonian <italic>Diospyros</italic>
species belong. We also present chromosome numbers and genome sizes of some additional New Caledonian species of <italic>Diospyros</italic>
; we wished to examine further the hypothesis that polyploidy (perhaps involving hybridization) might have played a role in producing diversity in this comparatively species-rich clade.</p>
</sec>
<sec sec-type="materials|methods" id="s0010"><label>2</label>
<title>Materials and methods</title>
<sec id="s0015"><label>2.1</label>
<title>Material</title>
<p>Material from New Caledonian <italic>Diospyros</italic>
species was collected by B. Turner (BT), J. Munzinger (JM), Yohan Pillon (YP) or Vanessa Hequet (VH). When fertile, a voucher was made with several duplicates sent to various herbaria. When sterile, one voucher per population was taken; this was compared to already existing collections in Noumea Herbarium (NOU) from the same location and referred to that species if similar. One putatively new species was detected while doing fieldwork for this project, here called <italic>D.</italic>
sp. Pic N’ga. Other Ebenaceae samples are from previous studies (<xref rid="b0070" ref-type="bibr">Duangjai et al., 2009</xref>
). Outgroup taxa and a few <italic>Diospyros</italic>
samples were taken from the Royal Botanic Gardens, Kew, DNA Bank (<ext-link ext-link-type="uri" xlink:href="http://apps.kew.org/dnabank/homepage.html" id="ir0005">http://apps.kew.org/dnabank/homepage.html</ext-link>
). Compared to the sampling of <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
, we added material of the following New Caledonian species: <italic>D. erudita</italic>
, <italic>D. glans</italic>
, <italic>D. impolita</italic>
, <italic>D. inexplorata</italic>
, <italic>D. margaretae</italic>
, <italic>D. tireliae</italic>
, <italic>D. tridentata</italic>
, <italic>D. trisulca</italic>
and <italic>D. veillonii</italic>
(for details see <xref rid="t0005" ref-type="table">Table 1</xref>
). The three un-sampled species from New Caledonia (<italic>D. fastidiosa</italic>
, <italic>D. nebulosa</italic>
and <italic>D. neglecta</italic>
) are rare and have not been seen after their description.</p>
</sec>
<sec id="s0020"><label>2.2</label>
<title>DNA extraction</title>
<p>For DNA extraction the sorbitol/high-salt CTAB method (<xref rid="b0385" ref-type="bibr">Tel-Zur et al., 1999</xref>
), modified for 2 ml micro-centrifuge tubes, was used. Tubes containing silica gel-dried material were frozen with liquid nitrogen (to keep material frozen during grinding to avoid enzymatic action) and then ground with glass-beads to a fine powder. Prior to extraction, ground material was washed three times with sorbitol buffer.</p>
</sec>
<sec id="s0025"><label>2.3</label>
<title>PCR and cycle sequencing</title>
<p>We sequenced four plastid regions: <italic>atpB</italic>
, <italic>rbcL</italic>
, <italic>trnK</italic>
–<italic>matK</italic>
(partial <italic>trnK</italic>
intron and complete <italic>matK</italic>
gene) and <italic>trnS</italic>
–<italic>trnG</italic>
, which collectively represent approximately 6.5 kb. Primers and PCR conditions are those of <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
. We added 136 accessions to the matrix of <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
.</p>
<p>Chloroplast-expressed glutamine synthetase (<italic>ncpGS</italic>
) was amplified with primers designed for this study (GScpDio1F and GScpDioR; <xref rid="t0020" ref-type="table">Table 4</xref>
). Initial <italic>Diospyros</italic>
sequences for primer design were obtained with the primers and PCR protocol of <xref rid="b0410" ref-type="bibr">Yockteng and Nadot (2004)</xref>
. Primers were situated at the end of exon 7 (forward) and beginning of exon 11 (reverse), amplifying a fragment between 700 and 715 bp (<xref rid="f0010" ref-type="fig">Fig. 2</xref>
). Primers used for PCR were also used for cycle sequencing (<xref rid="t0010 t0015" ref-type="table">Tables 2 and 3</xref>
).</p>
<p>Initial PCR products and sequences of <italic>PHYA</italic>
were obtained with the locus-specific primers of Mathews and Donoghue (1999; <italic>PHYA</italic>
upstream [2nd] and <italic>PHYA</italic>
downstream [1st]). As these primers were not specific enough, we cloned the PCR products (see Section <xref rid="s0030" ref-type="sec">2.4</xref>
) to be able to design <italic>Diospyros</italic>
-specific <italic>PHYA</italic>
PCR and sequencing primers (PhyADioF, PhyADioR, PhyADioFi and PhyADioRi; <xref rid="t0020" ref-type="table">Table 4</xref>
; <xref rid="f0015" ref-type="fig">Fig. 3</xref>
). However, as the new PCR primers designed for <italic>Diospyros</italic>
did not amplify consistently, we used a two-step amplification protocol. In the first PCR, the universal <italic>PHYA</italic>
primers were used, and then a second nested PCR was performed with the newly designed primers and the product from the first PCR as template. All primers are located in exon 1 of <italic>PHYA</italic>
flanking a region of 1187 bp in length. PCR conditions and composition are provided in <xref rid="t0010 t0015" ref-type="table">Tables 2 and 3</xref>
. For cycle-sequencing, we used the two internal primers and the external reverse primer.</p>
<p>PCR products were cleaned with a mixture of exonuclease I and alkaline phosphatase (10 units exo I and one unit FastAP, both from Thermo Scientific) and incubated at 37 °C for 45 min followed by 15 min at 80 °C to inactivate enzymes. Cycle sequencing reactions were performed with 0.8 μl BigDye Terminator v3.1 (AB, Live Technologies), 1.0 μl primer (3.2 μM), 1.6 μl 5× sequencing buffer and 6.6 μl cleaned-up PCR product using 35 cycles of 96 °C for 10 s, 50 °C for 5 s and 60 °C for 3 min. Sequences were produced on a capillary sequencer (3730 DNA Analyzer, AB, Life Technologies) following the manufacturer’s protocols.</p>
</sec>
<sec id="s0030"><label>2.4</label>
<title>Cloning</title>
<p>Cloning was needed to produce <italic>PHYA</italic>
from some accessions; these where than used for development of more specific primers. In addition, cloning of samples was necessary when we failed to obtain good sequences with the <italic>Diospyros</italic>
-specific primers. PCR products were obtained using the universal <italic>PHYA</italic>
primers, and after gel purification (Inivsorb Spin DNA Extraction Kit, Invitek), cleaned products were cloned using the pGEM-T Easy cloning system (Promega), following the manufacturer’s protocol. Cloned fragments were amplified using M13-f47 and M13-r48 primers and the following PCR conditions: initial denaturation 94 °C for 3 min, 35 cycles of denaturation 94 °C for 30 s, annealing 62 °C for 30 s and extension 72 °C for 2 min followed by a final extension at 72 °C for 7 min.</p>
</sec>
<sec id="s0035"><label>2.5</label>
<title>Sequence assembly, and editing, and phylogenetic analyses</title>
<p>Assembly and editing of sequences was done with the SeqMan Pro of the Lasergene v8.1 software package (DNASTAR); alignment was conducted with MUSCLE v3.8 (<xref rid="b0080" ref-type="bibr">Edgar, 2004</xref>
) and inspected visually using BioEdit v7.0.4 (<xref rid="b0170" ref-type="bibr">Hall, 1999</xref>
). Discrimination between the two copies of <italic>PHYA</italic>
that were recovered from some species was done based on the alignment, and the ‘wrong’ (highly divergent) copy was excluded from further analyses. To test congruence between the data sets, ILD (incongruence length difference) test (<xref rid="b0095" ref-type="bibr">Farris et al., 1994</xref>
) implemented in PAUP<sup>∗</sup>
v4b10 (<xref rid="b0365" ref-type="bibr">Swofford, 2003</xref>
; termed the “partition homogeny test”) was carried out with 100 replicates. To speed up this analysis, the neo-endemic clade (where resolution is low due to lack of variability and therefore congruence is unlikely to be detected) was reduced to two accessions (<italic>D</italic>
. sp. Pic N’ga BT318 and <italic>D. vieillardii</italic>
BT025). Results of the ILD test indicated congruence of the four plastid data sets, and therefore the plastid data sets were combined; jModeltest indicated the same model could be used in all analyses without partitioning. Phylogenetic analyses were performed using PAUP<sup>∗</sup>
v4b10 (<xref rid="b0365" ref-type="bibr">Swofford, 2003</xref>
) for maximum parsimony (MP) and RaxML (<xref rid="b0355" ref-type="bibr">Stamatakis, 2006</xref>
) for maximum likelihood (ML) analyses. For both methods, bootstrap with 1000 replicates was performed to estimate clade support. For Bayesian inference, the program BEAST v1.7.4 (<xref rid="b0065" ref-type="bibr">Drummond et al., 2012</xref>
) was used. Parsimony and Bayesian analyses were run on the Bioportal computer cluster of the University Oslo (<ext-link ext-link-type="uri" xlink:href="http://www.bioportal.uio.no" id="ir0010">www.bioportal.uio.no</ext-link>
), and likelihood analyses were run on CIPRS Science Gateway (<ext-link ext-link-type="uri" xlink:href="http://www.phylo.org/portal2/" id="ir0015">http://www.phylo.org/portal2/</ext-link>
; <xref rid="b0230" ref-type="bibr">Miller et al., 2010</xref>
). Estimation of evolutionary models and values was conducted with jModeltest v2.0.1 (<xref rid="b0055 b0160" ref-type="bibr">Darriba et al., 2012; Guindon and Gascuel, 2003</xref>
). For the Bayesian analyses the general time reversible nucleotide substitution model (GTR; <xref rid="b0380" ref-type="bibr">Tavaré, 1986</xref>
) with among site rate variation modelled with a gamma distribution (GTR + Γ) was used for <italic>ncpGS</italic>
, whereas for plastid data the same model was used but with a proportion of invariable sites (GTR + Γ + I). For <italic>PHYA</italic>
the Hasegawa–Kishino–Yano nucleotide substitution model (HKY; <xref rid="b0175" ref-type="bibr">Hasegawa et al., 1985</xref>
) was used with among site rate variation modelled with a gamma distribution and a proportion of invariable sites (HKY + Γ + I). Base frequencies (uniform), substitution rates between bases (gamma shape 10), alpha (gamma shape 10), kappa (gamma shape 10) and <italic>p</italic>
-inv (uniform) were inferred by Modeltest from each data set. We used a relaxed uncorrelated log-normal clock model (<xref rid="b0060" ref-type="bibr">Drummond et al., 2006</xref>
). As speciation model, we used a Yule model (<xref rid="b0125 b0415" ref-type="bibr">Gernhard, 2008; Yule, 1925</xref>
). For further details see <xref rid="s0100" ref-type="sec">Supplementary material S1</xref>
. Two independent Metropolis-coupled Markov chain Monte Carlo (MCMC) analyses each with 20 million generations were run sampling each 1000th generation. The initial 10% of trees obtained from each MCMC run were removed as burn in; the remaining trees of both runs were used to calculate a maximum clade credibility tree.</p>
</sec>
<sec id="s0040"><label>2.6</label>
<title>Dating the tree</title>
<p>To obtain an overarching dated tree, we used parts (<italic>atpB</italic>
and <italic>rbcL</italic>
sequences of Cornales and Ericales) of the data set of <xref rid="b0030" ref-type="bibr">Bell et al. (2010)</xref>
and combined it with our matrix. This matrix consisted of two plastid markers (<italic>atpB</italic>
and <italic>rbcL</italic>
), which were analysed as two partitions. Dating analyses were run in BEAST with an uncorrelated log-normal relaxed clock under the GTR + Γ + I model. The tree was calibrated with two fossils, <italic>Paleoenkianthus sayrevillensis</italic>
(90 myr; <xref rid="b0280" ref-type="bibr">Nixon and Crepet, 1993</xref>
) as minimum age for Ericales and <italic>A. cryptostoma</italic>
(34 myr; <xref rid="b0025" ref-type="bibr">Basinger and Christophel, 1985</xref>
) as minimum age for <italic>Diospyros</italic>
clade II. Both groups (Ericales and <italic>Diospyros</italic>
calde II) were defined as monophyletic, including the stem. Following tmrca (time of most recent common ancestor) settings used were: log normal prior distribution with a mean of 1.5, log standard deviation of 0.5 and an offset of 89 (Ericales) and 33 (<italic>Diospyros</italic>
clade II). Priors for the molecular clock were: ucld.stdev: log normal, mean 0.9, log stdev 1, initial value 0.5, mean in real space; ucld.mean: CTMC rate reference (<xref rid="b0100" ref-type="bibr">Ferreira and Suchard, 2008</xref>
, initial value 1. Details of settings for BEAST analysis are provided in Section <xref rid="s0035" ref-type="sec">2.5</xref>
(above) and <xref rid="s0100" ref-type="sec">Supplementary material S2</xref>
. In addition to the plastid marker dating, we also conducted an analysis with our combined data set. We used the same settings as for the Bayesian analysis, but we added two calibration points: <italic>A. cryptostoma</italic>
at 34 myr (<xref rid="b0025" ref-type="bibr">Basinger and Christophel, 1985</xref>
) as minimum age for <italic>Diospyros</italic>
clade II and the split of <italic>Diospyros</italic>
and its sister clade, <italic>Euclea</italic>
plus <italic>Royena</italic>
, 42 myr, which is the minimum age of that node based on dating exercises with the plastid markers. All settings for the molecular clock were the same as those for the plastid data set. The input file used for dating the combined analysis is provided in <xref rid="s0100" ref-type="sec">Supplementary material S3</xref>
.</p>
</sec>
<sec id="s0045"><label>2.7</label>
<title>Chromosome counts of <italic>Diospyros</italic>
</title>
<p>Chromosome preparations were made using Feulgen staining following the protocol from <xref rid="b0395" ref-type="bibr">Weiss-Schneeweiss et al. (2009)</xref>
. Root tips were collected from plant material growing in the Botanical Garden of the University of Vienna (HBV) and a private garden in New Caledonia. To arrest mitotic spindles, root tips were treated with 0.002 M 8-hydroxquinoline for 2 h at room temperature and 2 h at 4 °C (always in darkness because 8-hydroxquinoline is light sensitive). Pre-treated material was fixed for 12 h at room temperature in 3:1 ethanol:acetic acid and then stored at −20 °C until examined. Fixed root tips were washed in distilled water to remove fixative, hydrolysed in 5 N HCl for 30 min, washed again with distilled water and stained with Schiff’s reagent for approximately 2 h in the dark. Squash preparations were made under a coverslip in a drop of 45% acetic acid. Counts could only be made for few species because obtaining young, actively growing root-tips from New Caledonian <italic>Diospyros</italic>
is difficult. Collecting root-tips from forest trees and shrubs is not possible because there are too many roots in the soil to determine which is from the plant of interest. An alternative method is to grow seedlings in the lab/greenhouse. Obtaining seeds from tropical plants is not easy because these species do not produce fruit at a specific time of the year and flowering is diffuse (only few flowers produced at a time), so one would have to visit the plants regularly for at least 1 year to collect seed material. The logistics of this in process in New Caledonia were difficult. In addition, we found germination of seeds and maintenance of <italic>Diospyros</italic>
seedlings highly problematic. Fortunately, the material we were able to obtain is well distributed among the genome sizes obtained, so we can conclude more than would otherwise be possible.</p>
</sec>
<sec id="s0050"><label>2.8</label>
<title>Genome size estimations of <italic>Diospyros</italic>
</title>
<p>Genome size was determined using flow cytometry performed on leaf material. Fresh tissue was used from plants growing in the HBV. In addition, recently collected silica-gel dried material from New Caledonia was used for several measurements because it was not possible to transport fresh leaf material from New Caledonia to the laboratory. Samples were chopped in Otto I buffer (<xref rid="b0285" ref-type="bibr">Otto et al., 1981</xref>
) together with leaves of the internal standard species, <italic>Solanum pseudocapsicum</italic>
, 1C = 1.30 pg (<xref rid="b0390" ref-type="bibr">Temsch et al., 2010</xref>
) or <italic>Pisum sativum</italic>
´Kleine Rheinländeriń, 1C = 4.42 pg (<xref rid="b0155" ref-type="bibr">Greilhuber and Ebert, 1994</xref>
), according to the method of <xref rid="b0110" ref-type="bibr">Galbraith et al. (1983)</xref>
. The isolate was filtered through a 30 μm nylon mesh, and RNA was digested with 15 mg/l RNase A for 30 min at 37 °C. Subsequently, DNA was stained in propidium iodide (50 mg/l) supplemented with Otto II buffer (<xref rid="b0285" ref-type="bibr">Otto et al., 1981</xref>
). Mean fluorescence intensity of a total of 15,000 particles was measured with a CyFlow cytometer (Partec, Münster, Germany) equipped with a green laser (Cobolt Samba, Cobolt AB, Stockholm, Sweden); the 1C-value was calculated according to the formula: (MFI<sub>object</sub>
/MFI<sub>Standard</sub>
) × 1C-value<sub>Standard</sub>
, where MFI is the mean fluorescence intensity of the G1 nuclei population. Statistical significance of asymmetry between the results obtained from <italic>Diospyros</italic>
species belonging to clade III and those from clades VII–XI was tested using SPSS 15.0 (SPSS, Chicago; IL, USA) and the non-parametric Mann–Whitney U-test because of non-homogeneity of variances between the two groups of variables (Levene’s test for equality of variances, <italic>p </italic>
< 0.05).</p>
</sec>
</sec>
<sec sec-type="results" id="s0055"><label>3</label>
<title>Results</title>
<p>The data characteristics and statistics from the maximum parsimony analyses of all three individual and the combined data sets are provided in <xref rid="t0025" ref-type="table">Table 5</xref>
. Since the focus of this paper is the New Caledonian <italic>Diospyros</italic>
species from clade III, only results pertaining to this group will be discussed in detail. The other species have been included to (i) investigate the utility of these markers for resolving phylogenetic relationships within <italic>Diospyros</italic>
and (ii) further evaluate the hypothesis (proposed by <xref rid="b0070" ref-type="bibr">Duangjai et al., 2009</xref>
) that not all New Caledonian <italic>Diospyros</italic>
resulted from a single colonisation event.</p>
<sec id="s0060"><label>3.1</label>
<title>Plastid markers</title>
<p>Parsimony analysis of the plastid data set produced 210 equally parsimonious trees, one of which (randomly selected) is shown to demonstrate comparative levels of divergence (<xref rid="f0020" ref-type="fig">Fig. 4</xref>
). Clade names correspond to those of <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
. Resolution among the New Caledonian taxa of clade III is low, but monophyly of these taxa is strongly supported: bootstrap percentage MP (BMP) 88; bootstrap percentage ML (BML) 97; Bayesian posterior probability (BPP) 0.95. Furthermore, <italic>D. vieillardii</italic>
(BMP 99, BML 98, BPP 1.00) and its position as sister (BMP 97, BML 96, BPP 1.00) to the rest of the clade are well supported. Within the NC clade III, only one group of three taxa (<italic>D</italic>
. <italic>calciphila</italic>
, <italic>D</italic>
. <italic>inexplorata</italic>
and <italic>D</italic>
. sp. Pic N’ga) is supported in all three analyses (BMP 91, BML 92, BPP 1.00); this includes all accessions of each species forming unique clusters. There are a few more, weakly supported small groups in which individuals of one population fall together, but they are not consistent among the three analyses and fail to include all accessions of these species.</p>
</sec>
<sec id="s0065"><label>3.2</label>
<title>Low-copy nuclear markers</title>
<p>Nuclear markers contained proportionally more parsimony informative characters (<italic>ncpGS</italic>
2.7%, <italic>PHYA</italic>
1.2%) than the plastid markers (0.7%), but variation was still low. Some species form groups (<xref rid="f0025" ref-type="fig">Fig. 5</xref>
), but they lack bootstrap and Bayesian posterior probability support. Among the three methods of analysis used for the <italic>ncpGS</italic>
data set, Bayesian inference provides the best resolution (tree not shown), placing <italic>D. vieillardii</italic>
(BBP 0.99) sister (BPP 1.00) to the rest of the NC clade. The relationship between <italic>D. veillonii</italic>
and <italic>D. cherrieri</italic>
(BPP 0.84) is weakly supported, but their position as subsequent sister of the rest of this clade is well supported (BPP 0.99). All individuals of <italic>D. umbrosa</italic>
form a group with two individuals of <italic>D. trisulca</italic>
(BBP 0.91). This set of accessions is subsequently sister (BBP 0.95) to the rest of the clade, within which there is no resolution. In the <italic>PHYA</italic>
tree, there are only a few clades with strong support regardless of method of analysis. Clade III (BMP 100, BML 100, BPP 1.00) as monophyletic unit is confirmed, as well as the monophyly of NC clade III within it (BMP 77, BML 78, BPP 1.00). All included individuals of <italic>D. cherrieri</italic>
fall together (BMP 84, BML 81, BPP 1.00) in the <italic>PHYA</italic>
analyses. Only a single copy of <italic>ncpGS</italic>
was recovered from all accessions investigated, as well as from most of the accessions of <italic>PHYA</italic>
. Species from which two copies of <italic>PHYA</italic>
were obtained when cloned are found in clades IX, X and XI (<xref rid="f0020" ref-type="fig">Fig. 4</xref>
). The paralogous (divergent) copies of <italic>PHYA</italic>
were easily detected and excluded from the phylogenetic analyses.</p>
</sec>
<sec id="s0070"><label>3.3</label>
<title>Combined data set</title>
<p>The ILD test found the trees of the plastid and low-copy nuclear markers to be congruent with <italic>p</italic>
-values of 0.01, which indicates that combined analysis was appropriate. In trees inferred from the combined data set (<xref rid="f0030" ref-type="fig">Fig. 6</xref>
), species of clade III were highly supported (BMP 100, BML 100, BPP 1.00); they include the species of NC clade III, Indian Ocean islands, Thailand and Hawai‘i. <italic>Diospyros vera</italic>
is sister to <italic>D. sandwicensis</italic>
(BMP 100, BML 100, BPP 1.00) and then the NC clade III. NC clade III is moderately to well supported (BMP 83, BML 96, BPP 0.96). The position of <italic>D. vieillardii</italic>
(BMP 100, BML 99, BPP 1.00) as sister to the rest of the clade is strongly supported (BMP 92, BML 98, BPP 1.00). All accessions of each of the two species, <italic>D. umbrosa</italic>
(BMP < 70, BML 75, BPP 1.00) and <italic>D. flavocarpa</italic>
(BMP < 70, BML < 70, BPP 0.99), form unique groups, which together are sister (BMP 100, BML 100, BPP 1.00) to the rest of the group. A sister relationship between <italic>D. cherrieri</italic>
(BMP 96, BML 99, BPP 1.00) and <italic>D. veillonii</italic>
(BMP 78, BML 86, BPP 1.00) is supported (BMP 75, BML 88, BPP 1.00). A clade comprising <italic>D. calciphila</italic>
, <italic>D. inexplorata</italic>
(both on coralline substrates) and <italic>D</italic>
. sp. Pic N’ga (ultramafic substrate) is well supported (BMP 97, BML 99, BPP 1.00).</p>
</sec>
<sec id="s0075"><label>3.4</label>
<title>Dating analysis</title>
<p>We performed two dating analyses. The first one was based on a joint matrix of our plastid sequences together with the data set of <xref rid="b0030" ref-type="bibr">Bell et al. (2010)</xref>
, which included many families across the whole Ericales with Cornales as outgroup. This dating analysis was used to get age estimates for the crown node of Ebenaceae, the two subfamilies Ebenoideae and Lissocarpoideae, the split of the three genera of Ebenoideae (<italic>Diospyros</italic>
versus <italic>Euclea</italic>
/<italic>Royena</italic>
) and the main clades of <italic>Diospyros</italic>
. The second dating analysis was based on our combined data set, which was used to infer ages of clades and species within <italic>Diospyros</italic>
. The dating analysis of the over-arching matrix of plastid markers (<xref rid="s0100" ref-type="sec">Fig. S4</xref>
, <xref rid="s0100" ref-type="sec">Supplementary material</xref>
) indicates that the two subfamilies of Ebenaceae, Lissocarpoideae and Ebenoideae, diverged around 54 mya (42–65; 95% highest posterior density interval). The split of <italic>Diospyros</italic>
from its sister genera, <italic>Euclea</italic>
plus <italic>Royena</italic>
occurred around 42 mya (35–50). The following conclusions are based on the dating analysis of the combined data set (<xref rid="f0035" ref-type="fig">Fig. 7</xref>
). The Australian clade of <italic>Diospyros</italic>
(clade II, <xref rid="f0020" ref-type="fig">Fig. 4</xref>
), including five species from New Caledonia, separated from the rest of the genus around 34 mya (33–36), the New Caledonian and Australian members of this clade diverged around 20 mya (11–29). Divergence among the New Caledonian members began only about 6 mya (3–10). The two large main groups (clades V–XI and clade III, <xref rid="f0020" ref-type="fig">Fig. 4</xref>
) diverged about 32 mya (25–35). The last common ancestor of <italic>D. fasciculosa</italic>
and <italic>D. olen</italic>
existed around 15 mya (11–19). <italic>Diospyros olen</italic>
is around 5 myr (3–9) old and <italic>D. fasciculosa</italic>
about 6 myr (3–10). Lineages of clade III started to diversify about 19 mya (13–21). Lineages forming NC clade III arrived in New Caledonia around 9 mya (6–13). <italic>Diospyros vieillardii</italic>
is around 7 myr (5–10) old. The clade comprising <italic>D. cherrieri</italic>
and <italic>D. veillonii</italic>
is around 5 myr (3–8) old, and the two species separated around 3 mya (1–5). The clade including <italic>D. flavocarpa</italic>
, <italic>D. umbrosa</italic>
and one accession of <italic>D. trisulca</italic>
is 5 myr (3–7) old. <italic>Diospyros flavocarpa</italic>
is around 4 myr (3–6) old. The relationship between <italic>D. umbrosa</italic>
and <italic>D. trisulca</italic>
is not highly supported, but suggests an age of around 3 myr (2–5) for <italic>D. umbrosa</italic>
. The group comprising <italic>D. calciphila</italic>
, <italic>D. inexplorata</italic>
and <italic>D</italic>
. sp. Pic N’ga appears to be around 2 myr (1–3) old and started to diversify around 0.9 mya (0.5–2). Resolution between other species is too limited to say anything about their ages.</p>
</sec>
<sec id="s0080"><label>3.5</label>
<title>Chromosome counts and genome size</title>
<p>Chromosome counts made for <italic>Diospyros fasciculosa</italic>
, <italic>D. inconstans</italic>
, <italic><underline>D. macrocarpa</underline>
</italic>
, <italic>D. minimifolia</italic>
, <italic>D. pentamera</italic>
, <italic><underline>D. pustulata</underline>
</italic>
, <italic>D. texana</italic>
, <italic><underline>D. veillonii</underline>
</italic>
and <italic>D. yatesiana</italic>
indicate that they are all diploid, 2<italic>n </italic>
= 30. The counts from the underlined species are here reported for the first time in literature. The other counts confirm results of <xref rid="b0400" ref-type="bibr">White (1992)</xref>
.</p>
<p>Measurements of genome size showed differences among the New Caledonian species of <italic>Diospyros</italic>
. <italic>Diospyros olen</italic>
has with 1C = 0.86 pg, the smallest genome of the New Caledonian <italic>Diospyros</italic>
species examined, followed by <italic>D. fasciculosa</italic>
with 1C = 1.13 pg (both clade XI). The investigated species from the NC clade III have larger genomes (mean value 1C = 1.90 pg) than the two mentioned above (<xref rid="t0030" ref-type="table">Table 6</xref>
). We were not able to examine New Caledonian species from clade II. Finally, across whole genus <italic>Diospyros</italic>
there is a significant difference (Mann Whitney U test, <italic>p </italic>
< 0.001) in genome size between clade III on the one hand and clades VI–XI on the other (<xref rid="f0040" ref-type="fig">Fig. 8</xref>
). However, <italic>D. pentamera</italic>
of clade II has a comparatively large genome (1C = 1.97 pg).</p>
</sec>
</sec>
<sec sec-type="discussion" id="s0085"><label>4</label>
<title>Discussion</title>
<p>Previous phylogenetic studies of <italic>Diospyros</italic>
based on plastid markers demonstrated low levels of sequence divergence among New Caledonian species belonging to clade III (<xref rid="b0070" ref-type="bibr">Duangjai et al., 2009</xref>
), and inclusion of additional species in our investigation did not improve resolution in this group. Low-copy nuclear markers have been shown to be highly informative and useful for resolving phylogenetic relationships at lower taxonomic levels in some taxa (e.g. <italic>Passiflora</italic>
: <xref rid="b0410" ref-type="bibr">Yockteng and Nadot, 2004</xref>
; <italic>Paeonia</italic>
: <xref rid="b0375" ref-type="bibr">Tank and Sang, 2001</xref>
). The low-copy markers <italic>ncpGS</italic>
and <italic>PHYA</italic>
used here, however, did not improve resolution in this clade of 21 closely related species, thus preventing detection of hybrids and elucidation of geographical patterns (<xref rid="f0025" ref-type="fig">Fig. 5</xref>
). There are also examples where low-copy nuclear markers were not able to fully resolve phylogenetic relationships between closely related species, especially on islands (e.g. <xref rid="b0315 b0320 b0150" ref-type="bibr">Pillon et al., 2009a, 2013; Green et al., 2011</xref>
). Nonetheless, the analysis based on combined plastid and nuclear data provides some resolution of relationships within the NC clade III. Of the 21 entities included in the analyses, seven species and one unidentified taxon formed well defined and inclusive clusters (<xref rid="f0030" ref-type="fig">Fig. 6</xref>
). The remaining 14 species failed to form groups including all individuals of a particular species, but in many cases it was simply that some accessions were part of a polytomy and did not cluster consistently with any group.</p>
<p>In light of our results, members of the NC clade III appear little diverged but still form a strongly supported clade, which our dating analyses indicate are the result of recent rapid radiation. Only a few studies have examined the adaptive basis and processes involved in speciation in New Caledonia (e.g. <xref rid="b0325" ref-type="bibr">Pillon et al., 2009b</xref>
; <xref rid="b0260" ref-type="bibr">Murienne et al., 2009</xref>
). Rapid radiation has been observed in isolated areas such as islands (e.g. <xref rid="b0140 b0185" ref-type="bibr">Givnish et al., 2009; Knope et al., 2012</xref>
), high mountains (e.g. <xref rid="b0180" ref-type="bibr">Hughes and Eastwood, 2006</xref>
) and valleys (e.g. <xref rid="b0135 b0130 b0340" ref-type="bibr">Givnish et al., 2007, 2011; Richardson et al., 2001</xref>
). Island floras often show high levels of endemism and closely related species groups that result from a single colonisation event followed by rapid speciation, some of which have been hypothesised to represent adaptive radiations (e.g. Hawaiian silverswords, <xref rid="b0015" ref-type="bibr">Baldwin and Sanderson, 1998</xref>
; Hawaiian <italic>Bidens</italic>
, <xref rid="b0185" ref-type="bibr">Knope et al., 2012</xref>
; <italic>Araucaria</italic>
in New Caledonia, <xref rid="b0115" ref-type="bibr">Gaudeul et al., 2012</xref>
). The low levels of variation and resolution detected in the NC clade III prevent us from examining factors that may be promoting speciation on New Caledonia.</p>
<p>As all lineages of New Caledonian <italic>Diospyros</italic>
seem to have arrived relatively recent on this island, the terms paleo-endemics and neo-endemics used by <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
were not used here. The common ancestor of clade III diverged about 19 mya (<xref rid="f0035" ref-type="fig">Fig. 7</xref>
), and the earlier diverging species occur mainly in Africa and on islands of the western Indian Ocean (e.g. Madagascar). Our results in combination with the DIVA analysis from <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
indicate that, from there, this group spread eastwards via Southeast Asia, where it arrived around 15 mya, and then reached the Hawaiian Archipelago and New Caledonia around 9–10 mya. This time of colonisation is consistent with that found for other plant groups (reviewed in <xref rid="b0310" ref-type="bibr">Pillon, 2012</xref>
) and animals (e.g. <xref rid="b0270" ref-type="bibr">Nattier et al., 2011</xref>
). The close relationship of New Caledonian and Hawaiian endemic <italic>Diospyros</italic>
shows that migration around the Pacific Ocean has taken place, but to make more definite conclusions about the direction of dispersal, data from species present on other islands between New Caledonia and Hawai‘i are needed. In contrast to long-held hypotheses that many taxa are Gondwanan relicts (e.g. Lowry II, 1998; <xref rid="b0360" ref-type="bibr">Swenson et al., 2001</xref>
), our results suggest that all groups of New Caledonian <italic>Diospyros</italic>
are much younger than 37 myr (when New Caledonia re-emerged) and arrived, like many others, via long-distance dispersal (e.g. <xref rid="b0020 b0090 b0250" ref-type="bibr">Bartish et al., 2011; Espeland and Murienne, 2011; Murienne, 2009</xref>
).</p>
<p>The closely related species of the NC clade III are distinguishable from one another by morphological characters (e.g. leaf, flower, fruit and calyx characters), and many of them are found in different habitats (e.g. humid/dry, different substrate types, different elevations, etc.). Leaf morphology shows adaptation to the environment in which a species occurs (e.g. species found in dry habitats have sclerophyllous leaves; for details of species descriptions see <xref rid="b0400 b0405" ref-type="bibr">White, 1992, 1993</xref>
). In most plant groups, closely related species rarely occur in sympatry, but not in New Caledonia where this seems to be a common pattern in several groups (J. Munzinger pers. obs.), including <italic>Diospyros</italic>
. However, <italic>Diospyros</italic>
has been reported to be one of the few genera outside New Caledonia (e.g. Madagascar) with several co-occurring species (pers. comm. P.S. Ashton). The habitats occupied by the New Caledonian <italic>Diospyros</italic>
species belonging to clade III can be roughly divided into seven groups (<xref rid="t0035" ref-type="table">Table 7</xref>
). <italic>D. vieillardii</italic>
, a common species found all over Grande Terre and the islands north of the main island in maquis vegetation, occurs on a variety of substrates, including ultramafic. <italic>Diospyros umbrosa</italic>
/<italic>D. flavocarpa</italic>
are sister to the remainder of the clade excluding <italic>D. vieillardii</italic>
(<xref rid="f0030" ref-type="fig">Fig. 6</xref>
). <italic>D. umbrosa</italic>
occurs only on ultramafic substrates in comparatively humid forests mainly consisting of <italic>Nothofagus</italic>
and <italic>Araucaria</italic>
. <italic>D. flavocarpa</italic>
is found on schist in middle elevation forests in northeastern Grande Terre. <italic>Diospyros cherrieri</italic>
(a local endemic in dry forests on basalts at the western coast of Grande Terre, <xref rid="f0005" ref-type="fig">Fig. 1</xref>
I) and <italic>D. veillonii</italic>
(a local endemic in dry coastal forests on black clay on the western side of Grande Terre, <xref rid="f0005" ref-type="fig">Fig. 1</xref>
F) are together sister to the rest of the clade (minus those mentioned above). The clade comprising <italic>D. calciphila</italic>
, <italic>D. inexplorata</italic>
(littoral forests on coralline substrates) and <italic>D</italic>
. sp. from Pic N’ga (maquis on ultramafic substrate on Ile des Pins) is well supported. Relationships among all other members of the clade could not be resolved with the markers used, although most of them are morphologically and ecologically well defined. This phenomenon (morphological and ecological distinctiveness, but no resolution) is found, for example, in <italic>D. labillardieri</italic>
(lanceolate leaves, hanging branches; river edges in middle elevation forests on schist, <xref rid="f0005" ref-type="fig">Fig 1</xref>
D), <italic>D. pancheri</italic>
(obcordate pubescent leaves, hanging branches, humid forests at low elevation on ultramafic soils, <xref rid="f0005" ref-type="fig">Fig. 1</xref>
E) and many others. Due to the poor resolution of the phylogenetic trees, possible grouping of New Caledonian <italic>Diospyros</italic>
species according to their ecological niches remains untested.</p>
<p>A greater than threefold variation within the genome size of <italic>Diospyros</italic>
is observed, although the chromosome counts performed here and elsewhere indicates that they are diploid with 2<italic>n </italic>
= 30, and we hypothesize that the most recent common ancestor of <italic>Diospyros</italic>
had a large genome because species belonging to earlier diverging clades (e.g. <italic>E. divinorum</italic>
and <italic>D. pentamera</italic>
) have large genomes. Developing firmer ideas about evolution of genome size in <italic>Diospyros</italic>
would require many more measurements of species from throughout the phylogenetic tree, especially species from islands in the Indian and Pacific Ocean, which will be key to assessing evolution of genome size in NC clade III. The limited data available today suggest that polyploidy seems to be rare among wild <italic>Diospyros</italic>
species. The diversification of species of the NC clade III remains an overall poorly understood subject, despite our extensive efforts to find variation relevant to addressing these questions. It seems that we can eliminate polyploidy as one feature of their evolution, but the question of the involvement of hybridization cannot be eliminated without the use of more variable markers. To address patterns of speciation and factors promoting divergence, we will have to turn more markers used in population genetic studies, such as AFLPs, microsatellites or fingerprinting methods based on next generation sequencing methods.</p>
</sec>
</body>
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<title>Supplementary material</title>
<p><fig id="f0055" position="anchor"><label>Supplementary Figure 1</label>
<caption><p>Dated phylogeny of Ericales based on the a joint matrix the data set of Bell et al. (2010) together with our plastid sequences. Taxa from families other than Ebenaceae are collapsed to family level, taxa other than <italic>Diospyros</italic>
are collapsed to generic level. Multiple accessions of a species are collapsed to species level. The NC clade III part of the tree is mostly collapsed due to lack of support of respective nodes. Nodes which were calibrated with fossils are marked with a black dot. Yellow bars represent the 95% highest posterior density interval. New Caledonian taxa are coloured, red represents clade III NC.</p>
</caption>
<graphic xlink:href="fx2"></graphic>
</fig>
<supplementary-material content-type="local-data" id="m0005"><caption><title>Supplementary data 1</title>
<p>BEAST input file for the Bayesian analysis of the combined data set. For Bayesian analyses of the individual data sets we used the same settings as in the combined analysis for the respective data set.</p>
</caption>
<media xlink:href="mmc1.xml"></media>
</supplementary-material>
<supplementary-material content-type="local-data" id="m0010"><caption><title>Supplementary data 2</title>
<p>BEAST input file for dating analysis of the plastid marker data set from Bell et al. (2010) merged with our data.</p>
</caption>
<media xlink:href="mmc2.xml"></media>
</supplementary-material>
<supplementary-material content-type="local-data" id="m0015"><caption><title>Supplementary data 3</title>
<p>BEAST input file for dating analysis of the combined data set.</p>
</caption>
<media xlink:href="mmc3.xml"></media>
</supplementary-material>
</p>
</sec>
<ack id="ak005"><title>Acknowledgments</title>
<p>This work was funded by a grant from the Austrian Science Fund (FWF, Project-Number: P 22159-B16) awarded to R. Samuel. The authors thank the team of the Department of Systematic and Evolutionary Botany as well as the team of the Botanic Department of IRD Noumea for support with this study. Special thanks go to V. Klenja for the lab work. Thanks to the following persons for their help with lab work, field work and ideas to improve our manuscript: J.-P. Butin, C. Chambrey, G. Dagostini, E. Grasserbauer, V. Hequet, G. Kohl, D. & I. Létocart, F. Maghuly, W. Nigote, O. Paun, G. Schneeweiss, J. Schönenberger, H. Vandrot, Fam. Villegente, B. Wallnöfer and H. Weiss-Schneeweiss.</p>
</ack>
<fn-group><fn id="d32e9888"><label>☆</label>
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.</p>
</fn>
<fn id="s0095" fn-type="supplementary-material"><label>Appendix A</label>
<p>Supplementary data associated with this article can be found, in the online version, at <ext-link ext-link-type="doi" xlink:href="10.1016/j.ympev.2013.07.002" id="ir0025">http://dx.doi.org/10.1016/j.ympev.2013.07.002</ext-link>
.</p>
</fn>
</fn-group>
</back>
<floats-group><fig id="f0005"><label>Fig. 1</label>
<caption><p>Examples of <italic>Diospyros</italic>
species from New Caledonia (A–N) and Map of New Caledonia with collection points (O). A: <italic>D. vieillardii</italic>
; B: <italic>D. umbrosa</italic>
; C: <italic>D. flavocarpa</italic>
, D: <italic>D. labillardierei</italic>
; E: <italic>D. pancheri</italic>
, F: <italic>D. veillonii</italic>
; G: <italic>D. minimifolia</italic>
; H: <italic>D. pustulata</italic>
; I: <italic>D. cherrieri</italic>
; J: <italic>D. parviflora</italic>
; K: <italic>D.perplexa</italic>
; L: <italic>D. yaouhensis</italic>
; M: <italic>D. revolutissima</italic>
; N: <italic>D. glans</italic>
; O: Map of New Caledonia with sampling localities. Photographs taken by: C. Chambrey (I), V. Hequet (F, K, L), J. Munzinger (A, B, C, E, G, H, J, M, N) and B. Turner (D).</p>
</caption>
<graphic xlink:href="gr1"></graphic>
</fig>
<fig id="f0010"><label>Fig. 2</label>
<caption><p>Schematic diagram of exon 7–exon 11 of <italic>ncpGS</italic>
with primer positions and length of exons and introns. Numbers in parentheses give 5′ end of primers.</p>
</caption>
<graphic xlink:href="gr2"></graphic>
</fig>
<fig id="f0015"><label>Fig. 3</label>
<caption><p>Schematic diagram of exon 1 of <italic>phyA</italic>
with primer positions and length of exon. Numbers in parentheses give 5′ end of primers.</p>
</caption>
<graphic xlink:href="gr3"></graphic>
</fig>
<fig id="f0020"><label>Fig. 4</label>
<caption><p>One of 210 equally parsimonius trees of the plastid data set. Clades are named according to <xref rid="b0070" ref-type="bibr">Duangjai et al. (2009)</xref>
. Bold branches have more than 70% support in all three analysis. New Caledonian taxa are coloured, red represents clade III NC.</p>
</caption>
<graphic xlink:href="gr4"></graphic>
</fig>
<fig id="f0025"><label>Fig. 5</label>
<caption><p>Maximum parsimony trees inferred from the nuclear data sets, branch length scaled to same value on both trees. Bold branches have more than 70% support in all three analysis. New Caledonian taxa are coloured, red represents clade III NC. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)</p>
</caption>
<graphic xlink:href="gr5"></graphic>
</fig>
<fig id="f0030"><label>Fig. 6</label>
<caption><p>Baysian maximum clade credibility tree inferred from the combined data set. Bold branches have more than 70% support in all three analysis, nodes with at least one support value ⩾70% are indicated with blue dots (BPP/BMP/BML). New Caledonian taxa are coloured, red represents clade III NC. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)</p>
</caption>
<graphic xlink:href="gr6"></graphic>
</fig>
<fig id="f0035"><label>Fig. 7</label>
<caption><p>Chronogram based on the combined data set. Ages are given (in million years) for nodes with more than 0.85 BPP. Nodes which were calibrated are marked with a black dot. Yellow bars represent the 95% highest posterior density interval. New Caledonian taxa are coloured, red represents clade III NC.</p>
</caption>
<graphic xlink:href="gr7"></graphic>
</fig>
<fig id="f0040"><label>Fig. 8</label>
<caption><p>Boxplot of genome size differences between taxa from clade III and those from clades VII–XI.</p>
</caption>
<graphic xlink:href="gr8"></graphic>
</fig>
<table-wrap id="t0005" position="float"><label>Table 1</label>
<caption><p>Table of accessions; showing all individuals used in this study. Sequences provided by S. Duangjai are indicated.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th>Taxon</th>
<th>Acc.-nr.</th>
<th>Origin</th>
<th>Voucher</th>
<th>Herbarium</th>
<th><italic>atpB</italic>
</th>
<th><italic>rbcL</italic>
</th>
<th><italic>matK</italic>
& <italic>trnK</italic>
intron</th>
<th><italic>trnS</italic>
–<italic>trnG</italic>
</th>
<th><italic>ncpGS</italic>
</th>
<th><italic>PHYA</italic>
</th>
</tr>
</thead>
<tbody><tr><td><italic>D. abyssinica</italic>
(Hiern) F. White</td>
<td>K1672</td>
<td>Africa</td>
<td>Gilbert & Sebseke 8803</td>
<td>K</td>
<td>DQ923883</td>
<td>EU980646</td>
<td>DQ923990</td>
<td>EU981061</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. affinis</italic>
Thwaites</td>
<td>DY03</td>
<td>Sri Lanka</td>
<td>Yakandawala 03</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291310</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. affinis</italic>
</td>
<td>DY05</td>
<td>Sri Lanka</td>
<td>Yakandawala 05</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291311</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. affinis</italic>
</td>
<td>DY18</td>
<td>Sri Lanka</td>
<td>Yakandawala 18</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291312</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. affinis</italic>
</td>
<td>Eb179</td>
<td>Sri Lanka</td>
<td>Samuel s.n.</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291313</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. affinis</italic>
</td>
<td>Eb180</td>
<td>Sri Lanka</td>
<td>Samuel s.n.</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291314</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
cf. <italic>affinis</italic>
</td>
<td>S09</td>
<td>Sri Lanka</td>
<td>Samuel 09</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291315</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. andamanica</italic>
(Kurz) Bakh.</td>
<td>Eb002</td>
<td>Thailand</td>
<td>Duangjai 068</td>
<td>KUFF, W</td>
<td>DQ923884</td>
<td>EU980645</td>
<td>DQ923991</td>
<td>EU981060</td>
<td>KF291447</td>
<td>KF291624</td>
</tr>
<tr><td><italic>D. andamanica</italic>
</td>
<td>Eb104</td>
<td>Thailand</td>
<td>Duangjai 162</td>
<td>KUFF, W</td>
<td>DQ923950</td>
<td>EU980755</td>
<td>DQ924057</td>
<td>EU981170</td>
<td>KF291448</td>
<td>KF291625</td>
</tr>
<tr><td><italic>D. anisandra</italic>
S.F. Blake</td>
<td>W68</td>
<td>Guatemala</td>
<td>Wallnöfer 6012</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291316</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. anisandra</italic>
</td>
<td>W80</td>
<td>Guatemala</td>
<td>Frisch 2006-1</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291317</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. apiculata</italic>
Hiern</td>
<td>Eb006</td>
<td>Thailand</td>
<td>Duangjai 072</td>
<td>KUFF</td>
<td>EU980813</td>
<td>EU980647</td>
<td>EU980936</td>
<td>EU981062</td>
<td>KF291449</td>
<td>KF291626</td>
</tr>
<tr><td><italic>D. areolata</italic>
King & Gamble</td>
<td>Eb160</td>
<td>Brunei</td>
<td>Duangjai et al. 33</td>
<td>BRUN, W, WU</td>
<td>Duangjai unpubl</td>
<td>Duangjai unpubl</td>
<td>Duangjai unpubl</td>
<td>KF291318</td>
<td>KF291450</td>
<td>KF291627</td>
</tr>
<tr><td><italic>D. artanthifolia</italic>
Mart. ex Miq.</td>
<td>W15</td>
<td>Peru</td>
<td>Pirie 62</td>
<td>W</td>
<td>DQ923885</td>
<td>EU980648</td>
<td>DQ923992</td>
<td>EU981063</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. australis</italic>
(R.Br) Hiern</td>
<td>Eb205</td>
<td>Australia</td>
<td>Wallnöfer & Duangjai 13944</td>
<td>WU</td>
<td>DQ923887</td>
<td>EU980650</td>
<td>DQ923994</td>
<td>EU981065</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. australis</italic>
</td>
<td>K22548</td>
<td>Australia</td>
<td>Forster 7848</td>
<td>K</td>
<td>DQ923886</td>
<td>EU980649</td>
<td>DQ923993</td>
<td>EU981064</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. balansae</italic>
Guillaumin</td>
<td>M3556</td>
<td>New Caledonia</td>
<td>Munzinger 3556</td>
<td>NOU015466</td>
<td>EU980814</td>
<td>EU980651</td>
<td>EU980937</td>
<td>EU981066</td>
<td>KF291451</td>
<td>KF291628</td>
</tr>
<tr><td><italic>D. batocana</italic>
Hiern</td>
<td>K21210</td>
<td>Namibia</td>
<td>Steyl 88</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291319</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. batocana</italic>
</td>
<td>K22553</td>
<td>Zambia</td>
<td>Pope et al. 2196</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291320</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. bejaudii</italic>
Lecomte</td>
<td>Eb011</td>
<td>Thailand</td>
<td>Duangjai 075</td>
<td>KUFF, W</td>
<td>DQ923888</td>
<td>EU980652</td>
<td>DQ923995</td>
<td>EU981067</td>
<td>KF291452</td>
<td>KF291629</td>
</tr>
<tr><td><italic>D. bipindensis</italic>
Gürke</td>
<td>K22452</td>
<td>Gabon</td>
<td>Stone & Niangadouma 3554</td>
<td>MO</td>
<td>DQ923889</td>
<td>EU980653</td>
<td>DQ923996</td>
<td>EU981068</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. borbonica</italic>
I. Richardson</td>
<td>K23682</td>
<td>Reunion</td>
<td>Chase REU10042</td>
<td>REU, WU</td>
<td>EU980815</td>
<td>EU980654</td>
<td>EU980938</td>
<td>EU981069</td>
<td>KF291453</td>
<td>KF291630</td>
</tr>
<tr><td><italic>D. borneensis</italic>
Hiern</td>
<td>Eb015</td>
<td>Thailand</td>
<td>Duangjai 079</td>
<td>KUFF, W</td>
<td>DQ923890</td>
<td>EU980655</td>
<td>DQ923997</td>
<td>EU981070</td>
<td>KF291454</td>
<td>KF291631</td>
</tr>
<tr><td><italic>D. bourdillonii</italic>
Brandis</td>
<td>W82</td>
<td>India</td>
<td>DeFranceschi 18.12.2006</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291321</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. brandisiana</italic>
Kurz</td>
<td>Eb017</td>
<td>Thailand</td>
<td>Duangjai & Sinbumrung 007</td>
<td>KUFF, W</td>
<td>DQ923891</td>
<td>EU980656</td>
<td>DQ923998</td>
<td>EU981071</td>
<td>KF291455</td>
<td>KF291632</td>
</tr>
<tr><td><italic>D. brassica</italic>
F. White</td>
<td>M2898</td>
<td>New Caledonia</td>
<td>Munzinger 2898</td>
<td>NOU007949</td>
<td>DQ923892</td>
<td>EU980657</td>
<td>DQ923999</td>
<td>EU981072</td>
<td>KF291456</td>
<td>KF291633</td>
</tr>
<tr><td><italic>D. buxifolia</italic>
(Blume) Hiern</td>
<td>Eb018</td>
<td>Thailand</td>
<td>Duangjai 081</td>
<td>KUFF, W</td>
<td>EU980816</td>
<td>EU980658</td>
<td>EU980939</td>
<td>EU981073</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. buxifolia</italic>
</td>
<td>W85</td>
<td>India</td>
<td>DeFranceschi 18.12.2006</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291322</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. calciphila</italic>
F. White</td>
<td>BT314</td>
<td>New Caledonia</td>
<td>Munziner et al. 6650</td>
<td>MPU, NOU, P</td>
<td>KF291801</td>
<td>KF291860</td>
<td>KF291919</td>
<td>KF291323</td>
<td>KF291457</td>
<td>KF291634</td>
</tr>
<tr><td><italic>D. calciphila</italic>
</td>
<td>BT316</td>
<td>New Caledonia</td>
<td>Munziner et al. 6650</td>
<td>MPU, NOU, P</td>
<td>KF291802</td>
<td>KF291861</td>
<td>KF291920</td>
<td>KF291324</td>
<td>KF291458</td>
<td>KF291635</td>
</tr>
<tr><td><italic>D. calciphila</italic>
</td>
<td>BT317</td>
<td>New Caledonia</td>
<td>Munziner et al. 6653</td>
<td>MPU, NOU, P</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291459</td>
<td>KF291636</td>
</tr>
<tr><td><italic>D. calciphila</italic>
</td>
<td>YP124</td>
<td>New Caledonia</td>
<td>Pillon 124</td>
<td>NOU006325</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291460</td>
<td>KF291637</td>
</tr>
<tr><td><italic>D. capreifolia</italic>
Mart. ex Hiern</td>
<td>W09</td>
<td>French Guiana</td>
<td>Prévost & Sabatier 3476</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291325</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. carbonaria</italic>
Benoist</td>
<td>W10</td>
<td>French Guiana</td>
<td>Prévost & Sabatier 3470</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291326</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. caribaea</italic>
(A.DC.) Standl.</td>
<td>W65</td>
<td>Cuba</td>
<td>Abbott 19004</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291327</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. castanea</italic>
(Craib) Fletcher</td>
<td>Eb020</td>
<td>Thailand</td>
<td>Duangjai 083</td>
<td>KUFF, W</td>
<td>DQ923893</td>
<td>EU980660</td>
<td>DQ924000</td>
<td>EU981075</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. cauliflora</italic>
Blume</td>
<td>Eb024</td>
<td>Thailand</td>
<td>Duangjai 087</td>
<td>KUFF, W</td>
<td>DQ923894</td>
<td>EU980661</td>
<td>DQ924001</td>
<td>EU981076</td>
<td>KF291461</td>
<td>KF291638</td>
</tr>
<tr><td><italic>D. cavalcantei</italic>
Sothers</td>
<td>W22</td>
<td>French Guiana</td>
<td>Prévost et al. 4671</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291328</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. cayennensis</italic>
A.DC.</td>
<td>W03</td>
<td>French Guiana</td>
<td>Prévost 3430</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291329</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. celebica</italic>
Bakh.</td>
<td>K1242</td>
<td>Indonesia</td>
<td>Chase 1242</td>
<td>K</td>
<td>DQ923897</td>
<td>EU980664</td>
<td>DQ924004</td>
<td>EU981079</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. cherrieri</italic>
F. White</td>
<td>BT262</td>
<td>New Caledonia</td>
<td>Chambrey & Turner 16</td>
<td>NOU079551, WU062860</td>
<td>KF291803</td>
<td>KF291862</td>
<td>KF291921</td>
<td>KF291330</td>
<td>KF291463</td>
<td>KF291640</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>BT297</td>
<td>New Caledonia</td>
<td>Chambrey & Turner 17</td>
<td>NOU079547</td>
<td>KF291804</td>
<td>KF291863</td>
<td>KF291922</td>
<td>KF291331</td>
<td>KF291464</td>
<td>KF291641</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>VH3510</td>
<td>New Caledonia</td>
<td>Hequet 3510</td>
<td>NOU015245</td>
<td>EU980818</td>
<td>EU980665</td>
<td>EU980941</td>
<td>EU981080</td>
<td>KF291465</td>
<td>KF291642</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>VH3516</td>
<td>New Caledonia</td>
<td>Hequet 3516</td>
<td>NOU015251</td>
<td>EU980819</td>
<td>EU980666</td>
<td>EU980942</td>
<td>EU981081</td>
<td>KF291466</td>
<td>KF291643</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>VH3610</td>
<td>New Caledonia</td>
<td>Hequet 3610</td>
<td>NOU016962</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291467</td>
<td>KF291644</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>VH3640</td>
<td>New Caledonia</td>
<td>Hequet 3640</td>
<td>NOU017014</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291468</td>
<td>KF291645</td>
</tr>
<tr><td><italic>D. chrysophyllos</italic>
Poir.</td>
<td>K25758</td>
<td>Mauritius</td>
<td>Page 45</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291332</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. chrysophyllos</italic>
</td>
<td>K25769</td>
<td>Mauritius</td>
<td>Page 71</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291333</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. clementium</italic>
Bakh.</td>
<td>Eb154</td>
<td>Brunei</td>
<td>Duangjai et al. 24</td>
<td>BRUN, W, WU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291334</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. confertiflora</italic>
(Hiern) Bakh.</td>
<td>Eb028</td>
<td>Thailand</td>
<td>Duangjai 091</td>
<td>KUFF, W</td>
<td>DQ923898</td>
<td>EU980667</td>
<td>DQ924005</td>
<td>EU981082</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. consolatae</italic>
Chiov.</td>
<td>K1673</td>
<td>Africa</td>
<td>Beentje 2168</td>
<td>K</td>
<td>DQ923899</td>
<td>EU980668</td>
<td>DQ924006</td>
<td>EU981083</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. cooperi</italic>
(Hutchinson & Dalziel) F. White</td>
<td>K20604</td>
<td>Ghana</td>
<td>Merello et al. 1350</td>
<td>MO</td>
<td>DQ923900</td>
<td>EU980669</td>
<td>DQ924007</td>
<td>EU981084</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. crassinervis</italic>
(Krug & Urb.) Standl.</td>
<td>W23</td>
<td>Cuba</td>
<td>Rainer s.n.</td>
<td>W</td>
<td>DQ923901</td>
<td>EU980670</td>
<td>DQ924008</td>
<td>EU981085</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. curranii</italic>
Merr.</td>
<td>Eb031</td>
<td>Thailand</td>
<td>Duangjai 094</td>
<td>KUFF, W, WU</td>
<td>DQ923902</td>
<td>EU980671</td>
<td>DQ924009</td>
<td>EU981086</td>
<td>KF291469</td>
<td>KF291646</td>
</tr>
<tr><td><italic>D. dasyphylla</italic>
Kurz</td>
<td>Eb033</td>
<td>Thailand</td>
<td>Duangjai 096</td>
<td>KUFF, W</td>
<td>DQ923903</td>
<td>EU980672</td>
<td>DQ924010</td>
<td>EU981087</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. defectrix</italic>
Fletcher</td>
<td>Eb097</td>
<td>Thailand</td>
<td>Duangjai 155</td>
<td>KUFF, WU</td>
<td>KF291805</td>
<td>KF291864</td>
<td>KF291923</td>
<td>KF291335</td>
<td>KF291470</td>
<td>KF291647</td>
</tr>
<tr><td><italic>D. dendo</italic>
Welw. ex Hiern</td>
<td>K21197</td>
<td>Central African Republic</td>
<td>Harris & Fay 1594</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291336</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. dichroa</italic>
Sandwith</td>
<td>W13</td>
<td>French Guiana</td>
<td>Sabatier et al. 4457</td>
<td>W</td>
<td>DQ923904</td>
<td>EU980673</td>
<td>DQ924011</td>
<td>EU981088</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. dictyoneura</italic>
Hiern</td>
<td>Eb038</td>
<td>Thailand</td>
<td>Duangjai 100</td>
<td>KUFF, W</td>
<td>EU980674</td>
<td>EU980820</td>
<td>EU980943</td>
<td>EU981089</td>
<td>KF291471</td>
<td>KF291648</td>
</tr>
<tr><td><italic>D. diepenhorstii</italic>
Miq.</td>
<td>Eb042</td>
<td>Thailand</td>
<td>Duangjai 103</td>
<td>KUFF, W</td>
<td>DQ923905</td>
<td>EU980675</td>
<td>DQ924012</td>
<td>EU981090</td>
<td>KF291472</td>
<td>KF291649</td>
</tr>
<tr><td><italic>D. discolor</italic>
Willd.</td>
<td>Eb088</td>
<td>Thailand</td>
<td>Duangjai 146</td>
<td>KUFF, WU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291337</td>
<td>KF291473</td>
<td>KF291650</td>
</tr>
<tr><td><italic>D. ebenum</italic>
J. Koenig ex Retz</td>
<td>DY06</td>
<td>Sri Lanka</td>
<td>Yakandawala 06</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291338</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ebenum</italic>
</td>
<td>DY08</td>
<td>Sri Lanka</td>
<td>Yakandawala 08</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291339</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ebenum</italic>
</td>
<td>Eb174</td>
<td>Sri Lanka</td>
<td>Samuel s.n.</td>
<td>WU</td>
<td>EU980677</td>
<td>EU980821</td>
<td>EU980944</td>
<td>EU981092</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ebenum</italic>
</td>
<td>W83</td>
<td>India</td>
<td>Ramesh Diosass-2</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291340</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ebenum</italic>
</td>
<td>W84</td>
<td>India</td>
<td>DeFranceschi 21.12.2006</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291341</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. egrettarum</italic>
I. Richardson</td>
<td>K25788</td>
<td>Mauritius</td>
<td>Page 122</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291342</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ehretioides</italic>
Wall. ex G. Don</td>
<td>Eb043</td>
<td>Thailand</td>
<td>Duangjai 104</td>
<td>KUFF, W</td>
<td>DQ923907</td>
<td>EU980678</td>
<td>DQ924014</td>
<td>EU981093</td>
<td>KF291474</td>
<td>KF291651</td>
</tr>
<tr><td><italic>D. eriantha</italic>
Charmp. ex Benth</td>
<td>W63</td>
<td>Taiwan</td>
<td>Chung & Anderberg 1401</td>
<td>HAST</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291343</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. erudita</italic>
F. White</td>
<td>BT287</td>
<td>New Caledonia</td>
<td>Chambrey & Turner 20</td>
<td>NOU</td>
<td>KF291806</td>
<td>KF291865</td>
<td>KF291924</td>
<td>KF291344</td>
<td>KF291475</td>
<td>KF291652</td>
</tr>
<tr><td><italic>D. erudita</italic>
</td>
<td>M2359</td>
<td>New Caledonia</td>
<td>Munzinger et al. 2359</td>
<td>NOU003840</td>
<td>EU980845</td>
<td>EU980739</td>
<td>EU980968</td>
<td>EU981154</td>
<td>KF291476</td>
<td>KF291653</td>
</tr>
<tr><td><italic>D. erudita</italic>
/<italic>pustulata</italic>
</td>
<td>M3010</td>
<td>New Caledonia</td>
<td>Munziner et al. 3010</td>
<td>NOU008358</td>
<td>EU980841</td>
<td>EU980735</td>
<td>EU980964</td>
<td>EU981150</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
(F. Muell.) F. Muell.</td>
<td>BT014</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6617</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291477</td>
<td>KF291654</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT142</td>
<td>New Caledonia</td>
<td>MacKee 27341</td>
<td>NOU022840</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291478</td>
<td>KF291655</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT165</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291479</td>
<td>KF291656</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT166</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291480</td>
<td>KF291657</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT335</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291481</td>
<td>KF291658</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>M2127</td>
<td>New Caledonia</td>
<td>Munzinger 2127</td>
<td>NOU003604</td>
<td>DQ923908</td>
<td>EU980679</td>
<td>DQ924015</td>
<td>EU981094</td>
<td>KF291482</td>
<td>KF291659</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>YP243</td>
<td>New Caledonia</td>
<td>Pillon et al. 243</td>
<td>NOU010096</td>
<td>EU980822</td>
<td>EU980680</td>
<td>EU980945</td>
<td>EU981095</td>
<td>KF291483</td>
<td>KF291660</td>
</tr>
<tr><td><italic>D. ferox</italic>
Bakh.</td>
<td>Eb146</td>
<td>Brunei</td>
<td>Duangjai et al. 012</td>
<td>BRUN, W, WU</td>
<td>DQ923909</td>
<td>EU980681</td>
<td>DQ924016</td>
<td>EU981096</td>
<td>KF291484</td>
<td>KF291661</td>
</tr>
<tr><td><italic>D. ferruginescens</italic>
Bakh.</td>
<td>Eb143</td>
<td>Brunei</td>
<td>Duangjai et al. 007</td>
<td>BRUN, W, WU</td>
<td>DQ923911</td>
<td>EU980685</td>
<td>DQ924018</td>
<td>EU981100</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. filipendula</italic>
Pierre ex Lecomte</td>
<td>Eb048</td>
<td>Thailand</td>
<td>Duangjai 109</td>
<td>KUFF</td>
<td>DQ923912</td>
<td>EU980686</td>
<td>DQ924019</td>
<td>EU981101</td>
<td>KF291485</td>
<td>KF291662</td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
(Vieill. ex P. Parm.) F. White</td>
<td>BT126</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6625</td>
<td>NOU</td>
<td>KF291807</td>
<td>KF291866</td>
<td>KF291925</td>
<td>KF291345</td>
<td>KF291486</td>
<td>KF291663</td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
</td>
<td>BT127</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6625</td>
<td>NOU</td>
<td>KF291808</td>
<td>KF291867</td>
<td>KF291926</td>
<td>KF291346</td>
<td>KF291487</td>
<td>KF291664</td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
</td>
<td>BT156</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6632</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291488</td>
<td>KF291665</td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
</td>
<td>K20607</td>
<td>New Caledonia</td>
<td>McPherson & Lowry 18563</td>
<td>NOU022877</td>
<td>DQ923913</td>
<td>EU980687</td>
<td>DQ924020</td>
<td>EU981102</td>
<td>KF291489</td>
<td>KF291666</td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
</td>
<td>K20614</td>
<td>New Caledonia</td>
<td>Lowry et al. 5783</td>
<td>NOU023319</td>
<td>EU980870</td>
<td>EU980782</td>
<td>EU980993</td>
<td>EU981197</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
</td>
<td>M2235</td>
<td>New Caledonia</td>
<td>Munzinger 2235</td>
<td>NOU006659</td>
<td>EU980825</td>
<td>EU980688</td>
<td>EU980948</td>
<td>EU981103</td>
<td>KF291490</td>
<td>KF291667</td>
</tr>
<tr><td><italic>D. flavocarpa</italic>
</td>
<td>M2905</td>
<td>New Caledonia</td>
<td>Munzinger et al. 2905</td>
<td>NOU007977</td>
<td>EU980826</td>
<td>EU980689</td>
<td>EU980949</td>
<td>EU981104</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. fragrans</italic>
Gürke</td>
<td>K22454</td>
<td>Gabon</td>
<td>SIMAB 010610</td>
<td>MO</td>
<td>DQ923914</td>
<td>EU980690</td>
<td>DQ924021</td>
<td>EU981105</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. frutescens</italic>
Blume</td>
<td>Eb049</td>
<td>Thailand</td>
<td>Duangjai 110</td>
<td>KUFF, W</td>
<td>EU980827</td>
<td>EU980691</td>
<td>EU980950</td>
<td>EU981106</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. fulvopilosa</italic>
Fletcher</td>
<td>Eb052</td>
<td>Thailand</td>
<td>Duangjai 113</td>
<td>KUFF, W</td>
<td>DQ923915</td>
<td>EU980692</td>
<td>DQ924022</td>
<td>EU981107</td>
<td>KF291491</td>
<td>KF291668</td>
</tr>
<tr><td><italic>D. fuscovelutina</italic>
Baker</td>
<td>RF938</td>
<td>Madagascar</td>
<td>RF 938</td>
<td>W</td>
<td>DQ923979</td>
<td>EU980803</td>
<td>DQ924088</td>
<td>EU981218</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. gabunensis</italic>
Gürke</td>
<td>K22560</td>
<td>Tanzania</td>
<td>Bidgood et al. 2890</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291347</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. gilletii</italic>
De Wild</td>
<td>K21198</td>
<td>Cameroon</td>
<td>Harris & Fay 884</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291348</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. glandulosa</italic>
Lace</td>
<td>Eb053</td>
<td>Thailand</td>
<td>Duangjai 114</td>
<td>KUFF, W</td>
<td>DQ923916</td>
<td>EU980693</td>
<td>DQ924023</td>
<td>EU981108</td>
<td>KF291492</td>
<td>KF291669</td>
</tr>
<tr><td><italic>D. glans</italic>
F. White</td>
<td>BT019</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td>KF291809</td>
<td>KF291868</td>
<td>KF291927</td>
<td>KF291349</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. glans</italic>
</td>
<td>BT093</td>
<td>New Caledonia</td>
<td>Turner et al. 093</td>
<td>MPU</td>
<td>KF291810</td>
<td>KF291869</td>
<td>KF291928</td>
<td>KF291350</td>
<td>KF291493</td>
<td>KF291670</td>
</tr>
<tr><td><italic>D. glans</italic>
</td>
<td>BT094</td>
<td>New Caledonia</td>
<td>Turner et al. 094</td>
<td>MPU</td>
<td>KF291811</td>
<td>KF291870</td>
<td>KF291929</td>
<td>KF291351</td>
<td>KF291494</td>
<td>KF291671</td>
</tr>
<tr><td><italic>D. glaucifolia</italic>
Metcalf</td>
<td>K14256</td>
<td>China</td>
<td>Chase 14256</td>
<td>K</td>
<td>DQ923917</td>
<td>EU980694</td>
<td>DQ924024</td>
<td>EU981109</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
cf. <italic>gracilipes</italic>
Hiern</td>
<td>RF978</td>
<td>Madagascar</td>
<td>RNF 978</td>
<td>W</td>
<td>DQ923918</td>
<td>EU980695</td>
<td>DQ924025</td>
<td>EU981110</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. gracilis</italic>
Fletcher</td>
<td>Eb058</td>
<td>Thailand</td>
<td>Duangjai 019</td>
<td>BK, BKF, KUFF, WU</td>
<td>KF291812</td>
<td>KF291871</td>
<td>KF291930</td>
<td>KF291352</td>
<td>KF291495</td>
<td>KF291672</td>
</tr>
<tr><td><italic>D. greenweyi</italic>
F. White</td>
<td>K21205</td>
<td>Somalia</td>
<td>Friis et al. 4991</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291353</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. grisebachii</italic>
(Heirn) Standl.</td>
<td>W64</td>
<td>Cuba</td>
<td>Abbott 18937</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291354</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. guianensis</italic>
(Aubl.) Gürke</td>
<td>W14</td>
<td>French Guiana</td>
<td>Prévost & Sabatier 4029</td>
<td>W</td>
<td>DQ923919</td>
<td>EU980696</td>
<td>DQ924026</td>
<td>EU981111</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. guianensis</italic>
</td>
<td>W78</td>
<td>French Guiana</td>
<td>Mori 25921</td>
<td>NY, W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291355</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. hartmaniana</italic>
S. Knapp</td>
<td>K22455</td>
<td>Panama</td>
<td>McPherson & Richardson 15959</td>
<td>MO</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291356</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. impolita</italic>
F. White</td>
<td>BT102</td>
<td>New Caledonia</td>
<td>Schmid 5010</td>
<td>NOU019538</td>
<td>KF291813</td>
<td>KF291872</td>
<td>KF291931</td>
<td>KF291357</td>
<td>KF291496</td>
<td>KF291673</td>
</tr>
<tr><td><italic>D. impolita</italic>
</td>
<td>BT105</td>
<td>New Caledonia</td>
<td>Schmid 5010</td>
<td>NOU019538</td>
<td>KF291814</td>
<td>KF291873</td>
<td>KF291932</td>
<td>KF291358</td>
<td>KF291497</td>
<td>KF291674</td>
</tr>
<tr><td><italic>D. inconstans</italic>
Jacq.</td>
<td>W79</td>
<td>Ecuador</td>
<td>Rainer 1682</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291359</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. inexplorata</italic>
F. White</td>
<td>BT304</td>
<td>New Caledonia</td>
<td>MacKee 22791</td>
<td>NOU005818</td>
<td>KF291815</td>
<td>KF291874</td>
<td>KF291933</td>
<td>KF291360</td>
<td>KF291498</td>
<td>KF291675</td>
</tr>
<tr><td><italic>D. inexplorata</italic>
</td>
<td>BT311</td>
<td>New Caledonia</td>
<td>MacKee 22791</td>
<td>NOU005818</td>
<td>KF291816</td>
<td>KF291875</td>
<td>KF291934</td>
<td>KF291361</td>
<td>KF291499</td>
<td>KF291676</td>
</tr>
<tr><td><italic>D. insidiosa</italic>
Bakh.</td>
<td>Eb061</td>
<td>Thailand</td>
<td>Duangjai 120</td>
<td>KUFF, W</td>
<td>DQ923920</td>
<td>EU980697</td>
<td>DQ924027</td>
<td>EU981112</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. iturensis</italic>
(Gürke) Letouzey & F. White</td>
<td>K21204</td>
<td>Cameroon</td>
<td>Harris & Fay 1513</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291362</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. kaki</italic>
L.f.</td>
<td>K920</td>
<td>Japan</td>
<td>Chase 920</td>
<td>K</td>
<td>DQ923921</td>
<td>EU980698</td>
<td>DQ924028</td>
<td>EU981113</td>
<td>KF291500</td>
<td>KF291677</td>
</tr>
<tr><td><italic>D. kirkii</italic>
Hiern</td>
<td>K22551</td>
<td>Zimbabwe</td>
<td>Poilecot 7650</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291363</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. kupensis</italic>
Gosline</td>
<td>AR62</td>
<td>Cameroon</td>
<td>Russell 62</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td></td>
<td>KF291501</td>
<td>KF291678</td>
</tr>
<tr><td><italic>D. labillardierei</italic>
F. White</td>
<td>BT121</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6624</td>
<td>NOU</td>
<td>KF291817</td>
<td>KF291876</td>
<td>KF291935</td>
<td>KF291364</td>
<td>KF291502</td>
<td>KF291679</td>
</tr>
<tr><td><italic>D. labillardierei</italic>
</td>
<td>BT122</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6624</td>
<td>NOU</td>
<td>KF291818</td>
<td>KF291877</td>
<td>KF291936</td>
<td>KF291365</td>
<td>KF291503</td>
<td>KF291680</td>
</tr>
<tr><td><italic>D. labillardierei</italic>
</td>
<td>BT179</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291504</td>
<td>KF291681</td>
</tr>
<tr><td><italic>D. labillardierei</italic>
</td>
<td>K20763</td>
<td>New Caledonia</td>
<td>McPherson & Munzinger 18038</td>
<td>MO</td>
<td>DQ923922</td>
<td>EU980699</td>
<td>DQ924029</td>
<td>EU981114</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. labillardierei</italic>
</td>
<td>M2219</td>
<td>New Caledonia</td>
<td>Munzinger 2219</td>
<td>NOU006657</td>
<td>EU980828</td>
<td>EU980700</td>
<td>EU980951</td>
<td>EU981115</td>
<td>KF291505</td>
<td>KF291682</td>
</tr>
<tr><td><italic>D. labillardierei</italic>
</td>
<td>M3053</td>
<td>New Caledonia</td>
<td>Munzinger 3053</td>
<td>NOU008407</td>
<td>EU980829</td>
<td>EU980701</td>
<td>EU980952</td>
<td>EU981116</td>
<td>KF291506</td>
<td>KF291683</td>
</tr>
<tr><td><italic>D. lanceifolia</italic>
Roxb.</td>
<td>K1245</td>
<td>Indonesia</td>
<td>Chase 1245</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291366</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. leucomelas</italic>
Poir.</td>
<td>K25752</td>
<td>Mauritius</td>
<td>Page 16</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291367</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. lotus</italic>
L.</td>
<td>D16</td>
<td>Living coll. HBV</td>
<td>Turner D16</td>
<td>Living coll. HBV</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291507</td>
<td>KF291684</td>
</tr>
<tr><td><italic>D. lotus</italic>
</td>
<td>K965</td>
<td>Living coll. Kew 1882-3501</td>
<td>Chase 965</td>
<td>K</td>
<td>DQ923924</td>
<td>EU980703</td>
<td>DQ924031</td>
<td>EU981118</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
(Vieill.) Hiern</td>
<td>BT043</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291508</td>
<td>KF291685</td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
</td>
<td>BT044</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291509</td>
<td>KF291686</td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
</td>
<td>BT048</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291510</td>
<td>KF291687</td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
</td>
<td>BT049</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291511</td>
<td>KF291688</td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
</td>
<td>BT050</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291512</td>
<td>KF291689</td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
</td>
<td>M2014</td>
<td>New Caledonia</td>
<td>Munzinger 2014</td>
<td>NOU003637</td>
<td>EU980830</td>
<td>EU980704</td>
<td>EU980953</td>
<td>EU981119</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. macrocarpa</italic>
</td>
<td>M2829</td>
<td>New Caledonia</td>
<td>Munzinger 2829</td>
<td>NOU008233</td>
<td>DQ923925</td>
<td>EU980705</td>
<td>DQ924032</td>
<td>EU981120</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. maingayi</italic>
(Hiern) Bakh.</td>
<td>Eb073</td>
<td>Thailand</td>
<td>Duangjai 131</td>
<td>KUFF, W</td>
<td>DQ923926</td>
<td>EU980706</td>
<td>DQ924033</td>
<td>EU981121</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. malabarica</italic>
(Desr.) Kostel.</td>
<td>Eb066</td>
<td>Thailand</td>
<td>Duangjai 006</td>
<td>KUFF, W</td>
<td>EU980708</td>
<td>DQ923928</td>
<td>DQ924035</td>
<td>EU981123</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. malabarica</italic>
</td>
<td>K1247</td>
<td>Indonesia</td>
<td>Chase 1247</td>
<td>K</td>
<td>DQ923927</td>
<td>EU980707</td>
<td>DQ924034</td>
<td>EU981122</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. malabarica</italic>
</td>
<td>W47</td>
<td>South East Asia, cult. USA</td>
<td>Abbott 14325</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291368</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. mannii</italic>
Hiern</td>
<td>K20597</td>
<td>Ghana</td>
<td>Merello et al. 1348</td>
<td>MO</td>
<td>DQ923929</td>
<td>EU980709</td>
<td>DQ924036</td>
<td>EU981124</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. margaretae</italic>
F. White</td>
<td>YP1267</td>
<td>New Caledonia</td>
<td>Pillon 1267</td>
<td>NOU049432, WU062863</td>
<td>KF291819</td>
<td>KF291878</td>
<td>KF291937</td>
<td>KF291369</td>
<td>KF291513</td>
<td>KF291690</td>
</tr>
<tr><td><italic>D. maritima</italic>
Blume</td>
<td>Eb209</td>
<td>Malaysia</td>
<td>Wallnöfer 13948</td>
<td>W</td>
<td>DQ923930</td>
<td>EU980710</td>
<td>DQ924037</td>
<td>EU981125</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. melanida</italic>
Poir.</td>
<td>K25786</td>
<td>Mauritius</td>
<td>Page 112</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291370</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. melocarpa</italic>
F. White</td>
<td>K22457</td>
<td>Gabon</td>
<td>SIMAB 012319</td>
<td>MO</td>
<td>DQ923931</td>
<td>EU980711</td>
<td>DQ924038</td>
<td>EU981126</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. mespiliformis</italic>
Hochst. Ex A.DC.</td>
<td>Eb206</td>
<td>Tropical Africa</td>
<td>Wallnöfer & Duangjai 13945</td>
<td>W</td>
<td>DQ923932</td>
<td>EU980712</td>
<td>DQ924039</td>
<td>EU981127</td>
<td>KF291514</td>
<td>KF291691</td>
</tr>
<tr><td><italic>D. mespiliformis</italic>
</td>
<td>W60</td>
<td>Senegal</td>
<td>Prinz 2005-5</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291371</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. minimifolia</italic>
F. White</td>
<td>BT131</td>
<td>New Caledonia</td>
<td>Dagostini 203</td>
<td>NOU019556</td>
<td>KF291820</td>
<td>KF291879</td>
<td>KF291938</td>
<td>KF291372</td>
<td>KF291515</td>
<td>KF291692</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
</td>
<td>BT133</td>
<td>New Caledonia</td>
<td>Dagostini 203</td>
<td>NOU019556</td>
<td>KF291821</td>
<td>KF291880</td>
<td>KF291939</td>
<td>KF291373</td>
<td>KF291516</td>
<td>KF291693</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
</td>
<td>BT231</td>
<td>New Caledonia</td>
<td>Veillon 7206</td>
<td>NOU019554</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291517</td>
<td>KF291694</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
</td>
<td>BT264</td>
<td>New Caledonia</td>
<td>Chambrey & Turner 24</td>
<td>NOU079549, WU062872</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291518</td>
<td>KF291695</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
</td>
<td>M2214</td>
<td>New Caledonia</td>
<td>Munzinger 2214</td>
<td>NOU006263</td>
<td>EU980831</td>
<td>EU980714</td>
<td>EU980954</td>
<td>EU981129</td>
<td>KF291519</td>
<td>KF291696</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
</td>
<td>M2374</td>
<td>New Caledonia</td>
<td>Munzinger 2374</td>
<td>NOU006677</td>
<td>EU980832</td>
<td>EU980715</td>
<td>EU980955</td>
<td>EU981130</td>
<td>KF291520</td>
<td>KF291697</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
/<italic>pustulata</italic>
</td>
<td>BT143</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291521</td>
<td>KF291698</td>
</tr>
<tr><td><italic>D. mollis</italic>
Griff.</td>
<td>Eb074</td>
<td>Thailand</td>
<td>Duangjai 132</td>
<td>KUFF, W</td>
<td>DQ923934</td>
<td>EU980716</td>
<td>DQ924041</td>
<td>EU981131</td>
<td>KF291522</td>
<td>KF291699</td>
</tr>
<tr><td><italic>D. montana</italic>
Roxb.</td>
<td>Eb078</td>
<td>Thailand</td>
<td>Duangjai 136</td>
<td>KUFF, W</td>
<td>DQ923935</td>
<td>EU980717</td>
<td>DQ924042</td>
<td>EU981132</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. montana</italic>
</td>
<td>Eb130</td>
<td>Thailand</td>
<td>Duangjai & Sinbumrung 017</td>
<td>KUFF, W</td>
<td>DQ923943</td>
<td>EU980733</td>
<td>DQ924050</td>
<td>EU981148</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. myriophylla</italic>
(H. Perrier) G.E. Schatz & Lowry</td>
<td>W34</td>
<td>Madagascar</td>
<td>Sieder 209</td>
<td>W</td>
<td>DQ923974</td>
<td>EU980797</td>
<td>DQ924083</td>
<td>EU981212</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. natalensis</italic>
(Harv.) Brenan</td>
<td>K22554</td>
<td>Zambia</td>
<td>Bingham 10635</td>
<td>K</td>
<td>DQ923936</td>
<td>EU980718</td>
<td>DQ924043</td>
<td>EU981133</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. nigra</italic>
(J.F. Gmel.) Perrier</td>
<td>K212</td>
<td>Cult. Mexico</td>
<td>Chase 212</td>
<td>NCU</td>
<td>DQ923906</td>
<td>EU980676</td>
<td>DQ924013</td>
<td>EU981091</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. nigra</italic>
</td>
<td>K1146</td>
<td>Cult. Mexico</td>
<td>Chase 1146</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291374</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. obliquifolia</italic>
(Hiern ex Gürke) F. White</td>
<td>W91</td>
<td>Cameroon</td>
<td>Rainer 6.3.2007</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291375</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. oblonga</italic>
Wall. Ex G. Don.</td>
<td>Eb083</td>
<td>Thailand</td>
<td>Duangjai 141</td>
<td>KUFF, W</td>
<td>DQ923937</td>
<td>EU980719</td>
<td>DQ924044</td>
<td>EU981134</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. olen</italic>
Hiern</td>
<td>BT001</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6609</td>
<td>NOU</td>
<td>KF291822</td>
<td>KF291881</td>
<td>KF291940</td>
<td>KF291376</td>
<td>KF291523</td>
<td>KF291700</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT032</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291524</td>
<td>KF291701</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT034</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291525</td>
<td>KF291702</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT169</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6634</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291526</td>
<td>KF291703</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT302</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291527</td>
<td>KF291704</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>K20598</td>
<td>New Caledonia</td>
<td>Lowry et al. 5628</td>
<td>MO, NOU004840</td>
<td>DQ923938</td>
<td>EU980720</td>
<td>DQ924045</td>
<td>EU981135</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>M2827</td>
<td>New Caledonia</td>
<td>Munzinger 2827</td>
<td>NOU008235</td>
<td>EU980833</td>
<td>EU980721</td>
<td>EU980956</td>
<td>EU981136</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>YP153</td>
<td>New Caledonia</td>
<td>Pillon 153</td>
<td>NOU006438</td>
<td>EU980834</td>
<td>EU980722</td>
<td>EU980957</td>
<td>EU981137</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. oubatchensis</italic>
Kosterm.</td>
<td>BT160</td>
<td>New Caledonia</td>
<td>LeCore et al. 768</td>
<td>NOU079472</td>
<td>KF291823</td>
<td>KF291882</td>
<td>KF291941</td>
<td>KF291377</td>
<td>KF291528</td>
<td>KF291705</td>
</tr>
<tr><td><italic>D. oubatchensis</italic>
</td>
<td>BT161</td>
<td>New Caledonia</td>
<td>LeCore et al. 768</td>
<td>NOU079472</td>
<td>KF291824</td>
<td>KF291883</td>
<td>KF291942</td>
<td>KF291378</td>
<td>KF291529</td>
<td>KF291706</td>
</tr>
<tr><td><italic>D. oubatchensis</italic>
</td>
<td>M3118</td>
<td>New Caledonia</td>
<td>Munzinger 3118</td>
<td>NOU009675</td>
<td>EU980835</td>
<td>EU980723</td>
<td>EU980958</td>
<td>EU981138</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. oubatchensis</italic>
</td>
<td>M3333</td>
<td>New Caledonia</td>
<td>Munzinger 3333</td>
<td>NOU011201</td>
<td>EU980836</td>
<td>EU980724</td>
<td>EU980959</td>
<td>EU981139</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ovalifolia</italic>
Wight</td>
<td>DY10</td>
<td>Sri Lanka</td>
<td>Yakandawala 10</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291379</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pancheri</italic>
Kosterm.</td>
<td>BT027</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6619</td>
<td>NOU</td>
<td>KF291825</td>
<td>KF291884</td>
<td>KF291943</td>
<td>KF291380</td>
<td>KF291530</td>
<td>KF291707</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT028</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6619</td>
<td>NOU</td>
<td>KF291826</td>
<td>KF291885</td>
<td>KF291944</td>
<td>KF291381</td>
<td>KF291531</td>
<td>KF291708</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT029</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6619</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291532</td>
<td>KF291709</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT030</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6620</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291533</td>
<td>KF291710</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT031</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6620</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291534</td>
<td>KF291711</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT033</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6620</td>
<td>NOU</td>
<td>KF291827</td>
<td>KF291886</td>
<td>KF291945</td>
<td>KF291382</td>
<td>KF291535</td>
<td>KF291712</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT035</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6620</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291536</td>
<td>KF291713</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT076</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291537</td>
<td>KF291714</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>M2138</td>
<td>New Caledonia</td>
<td>Munzinger 2138</td>
<td>NOU003868</td>
<td>EU980837</td>
<td>EU980725</td>
<td>EU980960</td>
<td>EU981140</td>
<td>KF291538</td>
<td>KF291715</td>
</tr>
<tr><td><italic>D. pancheri</italic>
/<italic>parviflora</italic>
</td>
<td>M2338</td>
<td>New Caledonia</td>
<td>Munzinger 2338</td>
<td>NOU006586</td>
<td>EU980838</td>
<td>EU980726</td>
<td>EU980961</td>
<td>EU981141</td>
<td>KF291539</td>
<td>KF291716</td>
</tr>
<tr><td><italic>D. parviflora</italic>
(Schltr.) Bakh.</td>
<td>BT038</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td>KF291828</td>
<td>KF291887</td>
<td>KF291946</td>
<td>KF291383</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>BT039</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td>KF291829</td>
<td>KF291888</td>
<td>KF291947</td>
<td>KF291384</td>
<td>KF291540</td>
<td>KF291717</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>BT040</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291541</td>
<td>KF291718</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>BT042</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291542</td>
<td>KF291719</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>BT187</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6636</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291543</td>
<td>KF291720</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>M2037</td>
<td>New Caledonia</td>
<td>Munzinger 2037</td>
<td>NOU002519</td>
<td>EU980839</td>
<td>EU980727</td>
<td>EU980962</td>
<td>EU981142</td>
<td>KF291544</td>
<td>KF291721</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>M2071</td>
<td>New Caledonia</td>
<td>Munzinger 2071</td>
<td>NOU002608</td>
<td>EU980869</td>
<td>EU980776</td>
<td>EU980992</td>
<td>EU981191</td>
<td>KF291545</td>
<td>KF291722</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>M2708</td>
<td>New Caledonia</td>
<td>Munzinger 2708</td>
<td>NOU006658</td>
<td>EU980728</td>
<td>EU980840</td>
<td>EU980963</td>
<td>EU981143</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>M3035</td>
<td>New Caledonia</td>
<td>Munzinger 3035</td>
<td>NOU008397</td>
<td>EU980842</td>
<td>EU980736</td>
<td>EU980965</td>
<td>EU981151</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pentamera</italic>
(Woolls & F. Muell.) F. Muell.</td>
<td>K22549</td>
<td>Australia</td>
<td>Forster & Booth 25525</td>
<td>K</td>
<td>DQ923939</td>
<td>EU980729</td>
<td>DQ924046</td>
<td>EU981144</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. perplexa</italic>
F. White</td>
<td>BT004</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6611</td>
<td>NOU</td>
<td>KF291830</td>
<td>KF291889</td>
<td>KF291948</td>
<td>KF291385</td>
<td>KF291546</td>
<td>KF291723</td>
</tr>
<tr><td><italic>D. perplexa</italic>
</td>
<td>BT005</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6611</td>
<td>NOU</td>
<td>KF291831</td>
<td>KF291890</td>
<td>KF291949</td>
<td>KF291386</td>
<td>KF291547</td>
<td>KF291724</td>
</tr>
<tr><td><italic>D. perplexa</italic>
</td>
<td>BT009</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6611</td>
<td>NOU</td>
<td>KF291832</td>
<td>KF291891</td>
<td>KF291950</td>
<td>KF291387</td>
<td>KF291548</td>
<td>KF291725</td>
</tr>
<tr><td><italic>D. perplexa</italic>
</td>
<td>BT147</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6630</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291549</td>
<td>KF291726</td>
</tr>
<tr><td><italic>D. perplexa</italic>
</td>
<td>BT148</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6630</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291550</td>
<td>KF291727</td>
</tr>
<tr><td><italic>D. perplexa</italic>
</td>
<td>VH3614</td>
<td>New Caledonia</td>
<td>Hequet et al. 3614</td>
<td>NOU016957</td>
<td>EU980873</td>
<td>EU980786</td>
<td>EU980996</td>
<td>EU981201</td>
<td>KF291551</td>
<td>KF291728</td>
</tr>
<tr><td><italic>D. philippinensis</italic>
A.DC.</td>
<td>K1248</td>
<td>Indonesia</td>
<td>Chase 1248</td>
<td>K</td>
<td>DQ923940</td>
<td>EU980730</td>
<td>DQ924047</td>
<td>EU981145</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. philippinensis</italic>
</td>
<td>W62</td>
<td>Taiwan</td>
<td>Chung & Anderberg 1400</td>
<td>HAST</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291388</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pilosanthera</italic>
Blanco</td>
<td>Eb091</td>
<td>Thailand</td>
<td>Duangjai 149</td>
<td>KUFF, W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291389</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pilosiuscula</italic>
G. Don</td>
<td>Eb092</td>
<td>Thailand</td>
<td>Duangjai 150</td>
<td>KUFF, W</td>
<td>DQ923941</td>
<td>EU980731</td>
<td>DQ924048</td>
<td>EU981146</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. preussii</italic>
Gürke</td>
<td>LPJMO39</td>
<td>Cameroon</td>
<td>LPJMO39</td>
<td>YA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291390</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pruriens</italic>
Dalzell</td>
<td>W81</td>
<td>India</td>
<td>DeFranceschi 18.12.2006</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291391</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pseudomespilus</italic>
Mildbr.</td>
<td>K20606</td>
<td>Gabon</td>
<td>Walters et al. 956</td>
<td>MO</td>
<td>DQ923942</td>
<td>EU980732</td>
<td>DQ924049</td>
<td>EU981147</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. puncticulosa</italic>
Bakh.</td>
<td>Eb150</td>
<td>Brunei</td>
<td>Duangjai et al. 018</td>
<td>BRUN, W, WU</td>
<td>DQ923944</td>
<td>EU980734</td>
<td>DQ924051</td>
<td>EU981149</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. pustulata</italic>
F. White</td>
<td>BT113</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td>KF291833</td>
<td>KF291892</td>
<td>KF291951</td>
<td>KF291392</td>
<td>KF291552</td>
<td>KF291729</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>BT114</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td>KF291834</td>
<td>KF291893</td>
<td>KF291952</td>
<td>KF291393</td>
<td>KF291553</td>
<td>KF291730</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>BT136</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6629</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291554</td>
<td>KF291731</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>BT137</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6629</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291555</td>
<td>KF291732</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>BT257</td>
<td>New Caledonia</td>
<td>Cambrey & Turner 21</td>
<td>NOU079548, WU062871</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291556</td>
<td>KF291733</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>M3580</td>
<td>New Caledonia</td>
<td>Munzinger 3580</td>
<td>NOU016720</td>
<td>EU980843</td>
<td>EU980737</td>
<td>EU980966</td>
<td>EU981152</td>
<td>KF291557</td>
<td>KF291734</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>M3584</td>
<td>New Caledonia</td>
<td>Munzinger 3584</td>
<td>NOU016734</td>
<td>EU980844</td>
<td>EU980738</td>
<td>EU980967</td>
<td>EU981153</td>
<td>KF291558</td>
<td>KF291735</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>VH3638</td>
<td>New Caledonia</td>
<td>Hequet et al. 3638</td>
<td>NOU017016</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291559</td>
<td>KF291736</td>
</tr>
<tr><td><italic>D. pustulata</italic>
/<italic>yahouensis</italic>
</td>
<td>BT259</td>
<td>New Caledonia</td>
<td>Chambrey & Turner 26</td>
<td>WU062855</td>
<td>KF291835</td>
<td>KF291894</td>
<td>KF291953</td>
<td>KF291394</td>
<td>KF291560</td>
<td>KF291737</td>
</tr>
<tr><td><italic>D. racemosa</italic>
Roxb.</td>
<td>Eb106</td>
<td>Thailand</td>
<td>Duangjai 164</td>
<td>KUFF</td>
<td>EU980856</td>
<td>EU980759</td>
<td>EU980979</td>
<td>EU981174</td>
<td>KF291561</td>
<td>KF291738</td>
</tr>
<tr><td><italic>D. revaughanii</italic>
I. Richardson</td>
<td>K25760</td>
<td>Mauritius</td>
<td>Page 47</td>
<td>MAU</td>
<td>Duangjai unpubl</td>
<td>Duangjai unpubl</td>
<td>Duangjai unpubl</td>
<td>KF291395</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. revolutissima</italic>
F. White</td>
<td>BT116</td>
<td>New Caledonia</td>
<td>MacKee 22382</td>
<td>NOU023189</td>
<td>KF291836</td>
<td>KF291895</td>
<td>KF291954</td>
<td>KF291396</td>
<td>KF291562</td>
<td>KF291739</td>
</tr>
<tr><td><italic>D. revolutissima</italic>
</td>
<td>BT117</td>
<td>New Caledonia</td>
<td>MacKee 22382</td>
<td>NOU023189</td>
<td>KF291837</td>
<td>KF291896</td>
<td>KF291955</td>
<td>KF291397</td>
<td>KF291563</td>
<td>KF291740</td>
</tr>
<tr><td><italic>D. revolutissima</italic>
</td>
<td>BT218</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6640</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291564</td>
<td>KF291741</td>
</tr>
<tr><td><italic>D. revolutissima</italic>
</td>
<td>BT219</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6640</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291565</td>
<td>KF291742</td>
</tr>
<tr><td><italic>D. revolutissima</italic>
</td>
<td>YP204</td>
<td>New Caledonia</td>
<td>Pillon 204</td>
<td>NOU009155</td>
<td>EU980846</td>
<td>EU980740</td>
<td>EU980969</td>
<td>EU981155</td>
<td>KF291566</td>
<td>KF291743</td>
</tr>
<tr><td><italic>D. rhodocalyx</italic>
Kurz</td>
<td>Eb096</td>
<td>Thailand</td>
<td>Duangjai 154</td>
<td>KUFF, WU</td>
<td>KF291838</td>
<td>KF291897</td>
<td>KF291956</td>
<td>KF291398</td>
<td>KF291567</td>
<td>KF291744</td>
</tr>
<tr><td><italic>D. rhombifolia</italic>
Hemsl.</td>
<td>Eb129</td>
<td>Thailand</td>
<td>Duangjai & Sinbumrung 016</td>
<td>KUFF, W</td>
<td>DQ923945</td>
<td>EU980741</td>
<td>DQ924052</td>
<td>EU981156</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
cf. <italic>rhombifolia</italic>
</td>
<td>W76</td>
<td>Cult. USA, (South East Asia)</td>
<td>Abbott 20824</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291399</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ridleyi</italic>
Bakh.</td>
<td>Eb138</td>
<td>Brunei</td>
<td>Duangjai et al. 002</td>
<td>BRUN, W, WU</td>
<td>DQ923946</td>
<td>EU980742</td>
<td>DQ924053</td>
<td>EU981157</td>
<td>KF291568</td>
<td>KF291745</td>
</tr>
<tr><td><italic>D. rigida</italic>
Hiern</td>
<td>Eb140</td>
<td>Brunei</td>
<td>Duangjai et al. 004</td>
<td>BRUN, W, WU</td>
<td>DQ923947</td>
<td>EU980743</td>
<td>DQ924054</td>
<td>EU981158</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. ropourea</italic>
B. Walln.</td>
<td>W20</td>
<td>French Guiana</td>
<td>Wallnöfer 13459</td>
<td>W</td>
<td>DQ923948</td>
<td>EU980744</td>
<td>DQ924055</td>
<td>EU981159</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. salicifolia</italic>
Humb. & Bonpl. ex Willd.</td>
<td>W66</td>
<td>Guatemala</td>
<td>Abbott 19765</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291400</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. salicifolia</italic>
</td>
<td>W67</td>
<td>Guatemala</td>
<td>Abbott 19777</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291401</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. samoensis</italic>
A. Gray</td>
<td>Eb176</td>
<td>Cult. Hawaii Bot Garden</td>
<td>Kiehn s.n.</td>
<td>WU</td>
<td>EU980745</td>
<td>EU980847</td>
<td>EU980970</td>
<td>EU981160</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. samoensis</italic>
</td>
<td>M3593</td>
<td>Vanuatu</td>
<td>Munzinger 3593</td>
<td>NOU080070</td>
<td>EU980848</td>
<td>EU980746</td>
<td>EU980971</td>
<td>EU981161</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. samoensis</italic>
</td>
<td>M3624</td>
<td>Vanuatu</td>
<td>Munzinger 3624</td>
<td>NOU080138, NOU080139</td>
<td>EU980849</td>
<td>EU980747</td>
<td>EU980972</td>
<td>EU981162</td>
<td>KF291569</td>
<td>KF291746</td>
</tr>
<tr><td><italic>D. samoensis</italic>
</td>
<td>M3691</td>
<td>Vanuatu</td>
<td>Munzinger 3691</td>
<td>NOU</td>
<td>EU980850</td>
<td>EU980748</td>
<td>EU980973</td>
<td>EU981163</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. sandwicensis</italic>
(A.DC.) Fosberg</td>
<td>Eb175</td>
<td>Cult. Hawaii Bot Garden</td>
<td>Kiehn s.n.</td>
<td>WU</td>
<td>EU980851</td>
<td>EU980749</td>
<td>EU980974</td>
<td>EU981164</td>
<td>KF291570</td>
<td>KF291747</td>
</tr>
<tr><td><italic>D. scabra</italic>
(Chiov.) Cufod.</td>
<td>K21206</td>
<td>Ethiopia</td>
<td>Wondefrash & Tefera 9622</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291402</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. scalariformis</italic>
Fletcher</td>
<td>Eb172</td>
<td>Thailand</td>
<td>Duangjai & Sinbumrung s.n.</td>
<td>KUFF, W</td>
<td>EU980750</td>
<td>EU980852</td>
<td>EU980975</td>
<td>EU981165</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. senensis</italic>
Klotzsch</td>
<td>K22552</td>
<td>Zambia</td>
<td>Bingham 11092</td>
<td>K</td>
<td>EU980853</td>
<td>EU980751</td>
<td>EU980976</td>
<td>EU981166</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. squarrosa</italic>
Klotzsch</td>
<td>K21207</td>
<td>Somalia</td>
<td>Friis et al. 4894</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291417</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. squarrosa</italic>
</td>
<td>K22555</td>
<td>Zambia</td>
<td>Bingham & Downie 11465</td>
<td>K</td>
<td>EU980854</td>
<td>EU980752</td>
<td>EU980977</td>
<td>EU981167</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. styraciformis</italic>
King & Gamble</td>
<td>Eb149</td>
<td>Brunei</td>
<td>Duangjai et al. 017</td>
<td>BRUN, W, WU</td>
<td>DQ923949</td>
<td>EU980753</td>
<td>DQ924056</td>
<td>EU981168</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. sumatrana</italic>
Miq.</td>
<td>Eb099</td>
<td>Thailand</td>
<td>Duangjai 157</td>
<td>KUFF, W</td>
<td>EU980855</td>
<td>EU980754</td>
<td>EU980978</td>
<td>EU981169</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. tenuiflora</italic>
A.C.Sm.</td>
<td>W32</td>
<td>Brazil</td>
<td>Maas et al. 9186</td>
<td>NY, W</td>
<td>DQ923923</td>
<td>EU980702</td>
<td>DQ924030</td>
<td>EU981117</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. tesselaria</italic>
Poir.</td>
<td>K25751</td>
<td>Mauritius</td>
<td>Page 15</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291418</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. tetrandra</italic>
Hiern</td>
<td>W31</td>
<td>French Guiana</td>
<td>Prévost & Sabatier 4713</td>
<td>W</td>
<td>DQ923951</td>
<td>EU980756</td>
<td>DQ924058</td>
<td>EU981171</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. tetrasperma</italic>
Sw.</td>
<td>K14254</td>
<td>Mexico</td>
<td>Chase 14254</td>
<td>K, W</td>
<td>DQ923952</td>
<td>EU980757</td>
<td>DQ924059</td>
<td>EU981172</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. texana</italic>
Scheele</td>
<td>Eb208</td>
<td>Middle America</td>
<td>Wallnöfer & Duangjai 13946</td>
<td>W</td>
<td>DQ923953</td>
<td>EU980758</td>
<td>DQ924060</td>
<td>EU981173</td>
<td>KF291575</td>
<td>KF291752</td>
</tr>
<tr><td><italic>D. tireliae</italic>
F. White</td>
<td>M5725</td>
<td>New Caledonia</td>
<td>Munzinger 5725</td>
<td>NOU051026</td>
<td>KF291843</td>
<td>KF291902</td>
<td>KF291961</td>
<td>KF291419</td>
<td>KF291576</td>
<td>KF291753</td>
</tr>
<tr><td><italic>D. tridentata</italic>
F. White</td>
<td>BT202</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6639</td>
<td>NOU</td>
<td>KF291844</td>
<td>KF291903</td>
<td>KF291962</td>
<td>KF291420</td>
<td>KF291577</td>
<td>KF291754</td>
</tr>
<tr><td><italic>D. tridentata</italic>
</td>
<td>BT203</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6639</td>
<td>NOU</td>
<td>KF291845</td>
<td>KF291904</td>
<td>KF291963</td>
<td>KF291421</td>
<td>KF291578</td>
<td>KF291755</td>
</tr>
<tr><td><italic>D. trisulca</italic>
F. White</td>
<td>BT185</td>
<td>New Caledonia</td>
<td>Hequet (leg. Butin) 3820</td>
<td>NOU031344</td>
<td>KF291846</td>
<td>KF291905</td>
<td>KF291964</td>
<td>KF291422</td>
<td>KF291579</td>
<td>KF291756</td>
</tr>
<tr><td><italic>D. trisulca</italic>
</td>
<td>BT189</td>
<td>New Caledonia</td>
<td>Hequet (leg. Butin) 3820</td>
<td>NOU031344</td>
<td>KF291847</td>
<td>KF291906</td>
<td>KF291965</td>
<td>KF291423</td>
<td>KF291580</td>
<td>KF291757</td>
</tr>
<tr><td><italic>D. trisulca</italic>
</td>
<td>BT192</td>
<td>New Caledonia</td>
<td>Hequet (leg. Butin) 3820</td>
<td>NOU031344</td>
<td>KF291848</td>
<td>KF291907</td>
<td>KF291966</td>
<td>KF291424</td>
<td>KF291581</td>
<td>KF291758</td>
</tr>
<tr><td><italic>D. trisulca</italic>
</td>
<td>BT197</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6637</td>
<td>NOU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291582</td>
<td>KF291759</td>
</tr>
<tr><td><italic>D. trisulca</italic>
</td>
<td>M3179</td>
<td>New Caledonia</td>
<td>Munzinger 3179</td>
<td>NOU016896</td>
<td>EU980871</td>
<td>EU980784</td>
<td>EU980994</td>
<td>EU981199</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. trisulca</italic>
</td>
<td>M3260</td>
<td>New Caledonia</td>
<td>Munzinger 3260</td>
<td>NOU016891, WU062868</td>
<td>EU980872</td>
<td>EU980785</td>
<td>EU980995</td>
<td>EU981200</td>
<td>KF291583</td>
<td>KF291760</td>
</tr>
<tr><td><italic>D.</italic>
cf. <italic>ulo</italic>
Merr.</td>
<td>Eb152</td>
<td>Brunei</td>
<td>Duangjai et al. 021</td>
<td>BRUN, W, WU</td>
<td>EU980857</td>
<td>EU980760</td>
<td>EU980980</td>
<td>EU981175</td>
<td>KF291462</td>
<td>KF291639</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
F. White</td>
<td>BT065</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td>KF291849</td>
<td>KF291908</td>
<td>KF291967</td>
<td>KF291425</td>
<td>KF291584</td>
<td>KF291761</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT066</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291585</td>
<td>KF291762</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT071</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291586</td>
<td>KF291763</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT246</td>
<td>New Caledonia</td>
<td>McPherson 2144</td>
<td>NOU023234</td>
<td>KF291850</td>
<td>KF291909</td>
<td>KF291968</td>
<td>KF291426</td>
<td>KF291587</td>
<td>KF291764</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT247</td>
<td>New Caledonia</td>
<td>McPherson 2144</td>
<td>NOU023234</td>
<td>KF291851</td>
<td>KF291910</td>
<td>KF291969</td>
<td>KF291427</td>
<td>KF291588</td>
<td>KF291765</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT256</td>
<td>New Caledonia</td>
<td>McPherson 2144</td>
<td>NOU023234</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291589</td>
<td>KF291766</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>M2265</td>
<td>New Caledonia</td>
<td>Munzinger 2265</td>
<td>NOU006679</td>
<td>EU980858</td>
<td>EU980761</td>
<td>EU980981</td>
<td>EU981176</td>
<td>KF291590</td>
<td>KF291767</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>M2636</td>
<td>New Caledonia</td>
<td>Munzinger 2636</td>
<td>NOU006678</td>
<td>EU980859</td>
<td>EU980762</td>
<td>EU980982</td>
<td>EU981177</td>
<td>KF291591</td>
<td>KF291768</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>M2771</td>
<td>New Caledonia</td>
<td>Munzinger 2771</td>
<td>NOU007912</td>
<td>EU980860</td>
<td>EU980763</td>
<td>EU980983</td>
<td>EU981178</td>
<td>KF291592</td>
<td>KF291769</td>
</tr>
<tr><td><italic>D. undulata</italic>
Wall. Ex G. Don</td>
<td>Eb112</td>
<td>Thailand</td>
<td>Duangjai 170</td>
<td>KUFF, W</td>
<td>DQ923954</td>
<td>EU980764</td>
<td>DQ924061</td>
<td>EU981179</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. veillonii</italic>
F. White</td>
<td>BT224</td>
<td>New Caledonia</td>
<td>Veillon 7919</td>
<td>NOU019582</td>
<td>KF291852</td>
<td>KF291911</td>
<td>KF291970</td>
<td>KF291428</td>
<td>KF291593</td>
<td>KF291770</td>
</tr>
<tr><td><italic>D. veillonii</italic>
</td>
<td>BT229</td>
<td>New Caledonia</td>
<td>Veillon 7919</td>
<td>NOU019582</td>
<td>KF291853</td>
<td>KF291912</td>
<td>KF291971</td>
<td>KF291429</td>
<td>KF291594</td>
<td>KF291771</td>
</tr>
<tr><td><italic>D. veillonii</italic>
</td>
<td>M.sn.</td>
<td>New Caledonia</td>
<td>Munzinger s.n.</td>
<td>Living coll. Hortus Veillonii</td>
<td>EU980861</td>
<td>EU980765</td>
<td>EU980984</td>
<td>EU981180</td>
<td>KF291595</td>
<td>KF291772</td>
</tr>
<tr><td><italic>D. venosa</italic>
Wall ex A.DC</td>
<td>Eb119</td>
<td>Thailand</td>
<td>Duangjai 177</td>
<td>KUFF, W</td>
<td>DQ923955</td>
<td>EU980767</td>
<td>DQ924062</td>
<td>EU981182</td>
<td>KF291596</td>
<td>KF291773</td>
</tr>
<tr><td><italic>D. venosa</italic>
</td>
<td>Eb131</td>
<td>Thailand</td>
<td>Duangjai 059</td>
<td>KUFF, W</td>
<td>EU980862</td>
<td>EU980766</td>
<td>EU980985</td>
<td>EU981181</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. vera</italic>
(Lour.) A. Chev.</td>
<td>DY16</td>
<td>Sri Lanka</td>
<td>Yakandawala 16</td>
<td>PDA</td>
<td>EU980823</td>
<td>EU980682</td>
<td>EU980946</td>
<td>EU981097</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. vera</italic>
</td>
<td>Eb045</td>
<td>Thailand</td>
<td>Duangjai 106</td>
<td>KUFF</td>
<td>DQ923910</td>
<td>EU980683</td>
<td>DQ924017</td>
<td>EU981098</td>
<td>KF291597</td>
<td>KF291774</td>
</tr>
<tr><td><italic>D. vera</italic>
</td>
<td>K21193</td>
<td>Central African Republic</td>
<td>Harris & Fay 2032</td>
<td>K</td>
<td>EU980824</td>
<td>EU980684</td>
<td>EU980947</td>
<td>EU981099</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. vestita</italic>
Benoist</td>
<td>W01</td>
<td>French Guiana</td>
<td>Molino 1849</td>
<td>W</td>
<td>DQ923956</td>
<td>EU980768</td>
<td>DQ924063</td>
<td>EU981183</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. vieillardii</italic>
(Hiern) Kosterm.</td>
<td>BT025</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6618</td>
<td>NOU</td>
<td>KF291854</td>
<td>KF291913</td>
<td>KF291972</td>
<td>KF291430</td>
<td>KF291598</td>
<td>KF291775</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT026</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6618</td>
<td>NOU</td>
<td>KF291855</td>
<td>KF291914</td>
<td>KF291973</td>
<td>KF291431</td>
<td>KF291599</td>
<td>KF291776</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT055</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291600</td>
<td>KF291777</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT057</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291601</td>
<td>KF291778</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT099</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291602</td>
<td>KF291779</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT100</td>
<td>New Caledonia</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291603</td>
<td>KF291780</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT213</td>
<td>New Caledonia</td>
<td>MacKee 25141</td>
<td>NOU023242</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291604</td>
<td>KF291781</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT214</td>
<td>New Caledonia</td>
<td>MacKee 25141</td>
<td>NOU023242</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291605</td>
<td>KF291782</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT286</td>
<td>New Caledonia</td>
<td>Chambrey & Turner 13</td>
<td>NOU054004, WU062859</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291606</td>
<td>KF291783</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT325</td>
<td>New Caledonia</td>
<td>Munzinger et al. 6657</td>
<td>NOU, P</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291607</td>
<td>KF291784</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>M2106</td>
<td>New Caledonia</td>
<td>Munzinger 2106</td>
<td>NOU006676</td>
<td>EU980863</td>
<td>EU980769</td>
<td>EU980986</td>
<td>EU981184</td>
<td>KF291608</td>
<td>KF291785</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>M2776</td>
<td>New Caledonia</td>
<td>Munzinger 2776</td>
<td>NOU008207</td>
<td>EU980864</td>
<td>EU980770</td>
<td>EU980987</td>
<td>EU981185</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>M3476</td>
<td>New Caledonia</td>
<td>Munzinger 3476</td>
<td>NOU012947</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291609</td>
<td>KF291786</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>M3572</td>
<td>New Caledonia</td>
<td>Munzinger 3572</td>
<td>NOU016733</td>
<td>EU980866</td>
<td>EU980772</td>
<td>EU980989</td>
<td>EU981187</td>
<td>KF291610</td>
<td>KF291787</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>YP146</td>
<td>New Caledonia</td>
<td>Pillon 146</td>
<td>NOU006400</td>
<td>EU980867</td>
<td>EU980773</td>
<td>EU980990</td>
<td>EU981052</td>
<td>KF291611</td>
<td>KF291788</td>
</tr>
<tr><td><italic>D. virginiana</italic>
L.</td>
<td>K14255</td>
<td>USA</td>
<td>Chase 14255</td>
<td>K</td>
<td>DQ923957</td>
<td>EU980774</td>
<td>DQ924064</td>
<td>EU981189</td>
<td>KF291612</td>
<td>KF291789</td>
</tr>
<tr><td><italic>D. wallichii</italic>
King & Gamble ex King</td>
<td>Eb122</td>
<td>Thailand</td>
<td>Duangjai 180</td>
<td>KUFF, W</td>
<td>EU980868</td>
<td>EU980775</td>
<td>EU980991</td>
<td>EU981190</td>
<td>KF291613</td>
<td>KF291790</td>
</tr>
<tr><td><italic>D. wallichii</italic>
</td>
<td>Eb165</td>
<td>Brunei</td>
<td>Duangjai et al. 41</td>
<td>BRUN, W, WU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291614</td>
<td>KF291791</td>
</tr>
<tr><td><italic>D. winitii</italic>
Fletcher</td>
<td>Eb123</td>
<td>Thailand</td>
<td>Duangjai 181</td>
<td>KUFF, WU</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291615</td>
<td>KF291792</td>
</tr>
<tr><td><italic>D. yahouensis</italic>
(Schltr.) Kosterm.</td>
<td>BT237</td>
<td>New Caledonia</td>
<td>Schlechter 15059</td>
<td>P00057340</td>
<td>KF291856</td>
<td>KF291915</td>
<td>KF291974</td>
<td>KF291432</td>
<td>KF291616</td>
<td>KF291793</td>
</tr>
<tr><td><italic>D. yahouensis</italic>
</td>
<td>BT238</td>
<td>New Caledonia</td>
<td>Schlechter 15059</td>
<td>P00057340</td>
<td>KF291857</td>
<td>KF291916</td>
<td>KF291975</td>
<td>KF291433</td>
<td>KF291617</td>
<td>KF291794</td>
</tr>
<tr><td><italic>D. yahouensis</italic>
</td>
<td>BT239</td>
<td>New Caledonia</td>
<td>Schlechter 15059</td>
<td>P00057340</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291618</td>
<td>KF291795</td>
</tr>
<tr><td><italic>D. yahouensis</italic>
</td>
<td>VH3637</td>
<td>New Caledonia</td>
<td>Hequet et al. 3637</td>
<td>NOU017017</td>
<td>KF291858</td>
<td>KF291917</td>
<td>KF291976</td>
<td>KF291434</td>
<td>KF291619</td>
<td>KF291796</td>
</tr>
<tr><td><italic>D. yatesiana</italic>
Standl.</td>
<td>W27</td>
<td>Guatemala</td>
<td>Frisch s.n.</td>
<td>W</td>
<td>DQ923958</td>
<td>EU980777</td>
<td>DQ924065</td>
<td>EU981192</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp. Pic N’ga</td>
<td>BT318</td>
<td>New Caledonia</td>
<td>Munzinger 6065</td>
<td>NOU</td>
<td>KF291839</td>
<td>KF291898</td>
<td>KF291957</td>
<td>KF291404</td>
<td>KF291572</td>
<td>KF291749</td>
</tr>
<tr><td><italic>D.</italic>
sp. Pic N’ga</td>
<td>BT319</td>
<td>New Caledonia</td>
<td>Munzinger 6065</td>
<td>NOU</td>
<td>KF291840</td>
<td>KF291899</td>
<td>KF291958</td>
<td>KF291405</td>
<td>KF291573</td>
<td>KF291750</td>
</tr>
<tr><td><italic>D.</italic>
sp. Pic N’ga</td>
<td>BT320</td>
<td>New Caledonia</td>
<td>Munzinger 6065</td>
<td>NOU</td>
<td>KF291841</td>
<td>KF291900</td>
<td>KF291959</td>
<td>KF291406</td>
<td>KF291574</td>
<td>KF291751</td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>FS1637</td>
<td>Madagascar</td>
<td>Fischer & Sieder 1637</td>
<td>W</td>
<td>DQ923959</td>
<td>EU980778</td>
<td>DQ924066</td>
<td>EU981193</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>FS2217</td>
<td>Madagascar</td>
<td>Fischer & Sieder 2217</td>
<td>W</td>
<td>DQ923960</td>
<td>EU980779</td>
<td>DQ924067</td>
<td>EU981194</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>K20600</td>
<td>Madagascar</td>
<td>Rabenantoandro et al. 1246</td>
<td>MO</td>
<td>DQ923961</td>
<td>EU980780</td>
<td>DQ924068</td>
<td>EU981195</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>K20601</td>
<td>Madagascar</td>
<td>Rabevohitra et al. 3660</td>
<td>MO</td>
<td>DQ923973</td>
<td>EU980796</td>
<td>DQ924082</td>
<td>EU981211</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>K20613</td>
<td>Zambia</td>
<td>Zimba et al. 893</td>
<td>MO</td>
<td>DQ923962</td>
<td>EU980781</td>
<td>DQ924069</td>
<td>EU981196</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>K20616</td>
<td>Ghana</td>
<td>Schmidt et al. 2207</td>
<td>MO</td>
<td>DQ923963</td>
<td>EU980783</td>
<td>DQ924070</td>
<td>EU981198</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>K25759</td>
<td>Mauritius</td>
<td>Page 46</td>
<td>MAU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291403</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>RF958</td>
<td>Madagascar</td>
<td>RNF 958</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291407</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>RF959</td>
<td>Madagascar</td>
<td>RNF 959</td>
<td>W</td>
<td>DQ923980</td>
<td>EU980804</td>
<td>DQ924089</td>
<td>EU981219</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>RF970</td>
<td>Madagascar</td>
<td>RNF 970</td>
<td>W</td>
<td>DQ923964</td>
<td>EU980787</td>
<td>DQ924071</td>
<td>EU981202</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S10</td>
<td>Sri Lanka</td>
<td>Samuel 10</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291408</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S12</td>
<td>Sri Lanka</td>
<td>Samuel 12</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291409</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S18</td>
<td>Sri Lanka</td>
<td>Samuel 18</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291410</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S22</td>
<td>Sri Lanka</td>
<td>Samuel 22</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291411</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S25</td>
<td>Sri Lanka</td>
<td>Samuel 25</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291412</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S26</td>
<td>Sri Lanka</td>
<td>Samuel 26</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291413</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>S28</td>
<td>Sri Lanka</td>
<td>Samuel 28</td>
<td>PDA</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291414</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>W33</td>
<td>Madagascar</td>
<td>Sieder 440</td>
<td>W</td>
<td>KF291842</td>
<td>KF291901</td>
<td>KF291960</td>
<td>KF291415</td>
<td>KF291571</td>
<td>KF291748</td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>W36</td>
<td>Madagascar</td>
<td>Sieder et al. 258</td>
<td>W</td>
<td>DQ923965</td>
<td>EU980788</td>
<td>DQ924072</td>
<td>EU981203</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>D.</italic>
sp.</td>
<td>W77</td>
<td>Madagascar</td>
<td>Sieder et al. 3079</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291416</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea crispa</italic>
(Thunb.) Gürke</td>
<td>Eb202</td>
<td>Living coll. HBV (EB 4/2)</td>
<td>Wallnöfer 13949</td>
<td>W</td>
<td>DQ923966</td>
<td>EU980789</td>
<td>DQ924073</td>
<td>EU981204</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea crispa</italic>
</td>
<td>K21188</td>
<td>Malawi</td>
<td>Chapman & Chapman 8085</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291435</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea divinorum</italic>
Hiern</td>
<td>Eb201</td>
<td>Cult. HBV (EB 2/1, Salisburg 69)</td>
<td>Wallnöfer & Duangjai 13947</td>
<td>W</td>
<td>DQ923967</td>
<td>EU980790</td>
<td>DQ924074</td>
<td>EU981205</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea natalensis</italic>
A.DC.</td>
<td>K21186</td>
<td>Zimbabwe</td>
<td>Timberlake & Cunliffe 4389</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291436</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea natalensis</italic>
</td>
<td>W08</td>
<td>South Africa</td>
<td>Kurzweil E514</td>
<td>W</td>
<td>DQ923968</td>
<td>EU980791</td>
<td>DQ924075</td>
<td>EU981206</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea pseudobenus</italic>
E. Mey. ex A.DC</td>
<td>K21190</td>
<td>Namibia</td>
<td>Ward 9205</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291437</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea racemosa</italic>
L.</td>
<td>K21183</td>
<td>Somalia</td>
<td>Thulin 10739</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291438</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea</italic>
sp.</td>
<td>W58</td>
<td>Tanzania</td>
<td>Kutalek 1-2001</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291439</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea</italic>
sp.</td>
<td>W59</td>
<td>Tanzania</td>
<td>Mbeyela 2-2001</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291440</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Euclea undulata</italic>
Thunb.</td>
<td>Eb200</td>
<td>Cult. HBV (EB 5/2, 1973)</td>
<td>Wallnöfer 13897</td>
<td>W</td>
<td>EU980874</td>
<td>EU980792</td>
<td>DQ924076</td>
<td>EU981207</td>
<td>KF291620</td>
<td>KF291797</td>
</tr>
<tr><td><italic>Royena cordata</italic>
E. Mey ex A.DC</td>
<td>K1144</td>
<td>South Africa</td>
<td>Chase 1144</td>
<td>K</td>
<td>DQ923975</td>
<td>EU980799</td>
<td>DQ924084</td>
<td>EU981214</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Royena glabra</italic>
L.</td>
<td>W05</td>
<td>South Africa</td>
<td>Kurzweil 2097</td>
<td>W</td>
<td>DQ923976</td>
<td>EU980800</td>
<td>DQ924085</td>
<td>EU981215</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Royena lucida</italic>
L.</td>
<td>Eb203</td>
<td>South Africa</td>
<td>Wallnöfer & Duangjai 13943</td>
<td>W</td>
<td>DQ923977</td>
<td>EU980801</td>
<td>DQ924086</td>
<td>EU981216</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Royena lucida</italic>
</td>
<td>W06</td>
<td>South Africa</td>
<td>Kurzweil E513</td>
<td>W</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291442</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Royena lycioides</italic>
Desf. Ex A.DC</td>
<td>K977</td>
<td>South Africa</td>
<td>Chase 977</td>
<td>K</td>
<td>DQ923978</td>
<td>EU980802</td>
<td>DQ924087</td>
<td>EU981217</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Royena</italic>
sp.</td>
<td>K1145</td>
<td>South Africa</td>
<td>Chase 1145</td>
<td>K</td>
<td>KF291859</td>
<td>KF291918</td>
<td>KF291977</td>
<td>KF291444</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Royena whyteana</italic>
Hiern</td>
<td>Eb177</td>
<td>Africa</td>
<td>Kiehn s.n.</td>
<td>WU</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291443</td>
<td>KF291622</td>
<td>KF291799</td>
</tr>
<tr><td><italic>Royena zombensis</italic>
B.L. Burtt</td>
<td>K22558</td>
<td>Tanzania</td>
<td>Abdallah & Vollesen 95/106</td>
<td>K</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>Duangjai unpubl.</td>
<td>KF291445</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Lissocarpa benthamii</italic>
Gürke</td>
<td>W61</td>
<td>Venezuela</td>
<td>Berry et al. 7217</td>
<td>PORT</td>
<td>DQ923969</td>
<td>EU980793</td>
<td>DQ924077</td>
<td>EU981208</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Lissocarpa guianensis</italic>
Gleason</td>
<td>W04</td>
<td>Guyana</td>
<td>Arets s.n.</td>
<td>U</td>
<td>DQ923970</td>
<td>EU980794</td>
<td>DQ924078</td>
<td>EU981209</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Lissocarpa stenocarpa</italic>
Steyerm.</td>
<td>K20609</td>
<td>Peru</td>
<td>Vásquez & Ortiz-Gentry 25233</td>
<td>MO</td>
<td>DQ923971</td>
<td>EU980795</td>
<td>DQ924079</td>
<td>EU981210</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Argania spinosa</italic>
(L.) Skeels</td>
<td>K978</td>
<td>Morocco</td>
<td>Chase 978</td>
<td>K</td>
<td>DQ923981</td>
<td>EU980805</td>
<td>DQ924090</td>
<td>KF291308</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Cleyera japonica</italic>
Thunb.</td>
<td>K1690</td>
<td>Japan</td>
<td>Chase 1690</td>
<td>K</td>
<td>DQ923985</td>
<td>EU980811</td>
<td>DQ924094</td>
<td>KF291309</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Halesia carolina</italic>
L.</td>
<td>K910</td>
<td>USA</td>
<td>Chase 910</td>
<td>K</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291621</td>
<td>KF291798</td>
</tr>
<tr><td><italic>Madhuca macrophylla</italic>
(Hassk.) H.J. Lam</td>
<td>K1363</td>
<td>Cult. Indonesia</td>
<td>Chase 1363</td>
<td>K</td>
<td>DQ923982</td>
<td>EU980806</td>
<td>DQ924091</td>
<td>KF291441</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Styrax benzoin</italic>
Dryand.</td>
<td>K1371</td>
<td>Indonesia</td>
<td>Chase 1371</td>
<td>K</td>
<td>DQ923989</td>
<td>EU980809</td>
<td>DQ924098</td>
<td>KF291446</td>
<td></td>
<td></td>
</tr>
<tr><td><italic>Styrax officinalis</italic>
L.</td>
<td>K872</td>
<td>Living coll. RGB Kew 1973-14474</td>
<td>Chase 872</td>
<td>K</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>KF291623</td>
<td>KF291800</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t0010" position="float"><label>Table 2</label>
<caption><p>PCR reactions.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th colspan="2"><italic>ncpGS</italic>
</th>
<th colspan="2">1st <italic>phyA</italic>
</th>
<th colspan="2">2nd <italic>phyA</italic>
</th>
</tr>
</thead>
<tbody><tr><td>18 μl</td>
<td>1.1xReddyMix (Thermo Scientific)</td>
<td>18 μl</td>
<td>1.1xReddyMix (Thermo Scientific)</td>
<td>18 μl</td>
<td>1.1xReddyMix (Thermo Scientific)</td>
</tr>
<tr><td>0.4 μl</td>
<td>Primer GScpDio1F (20 pM)</td>
<td>0.4 μl</td>
<td>Primer <italic>PHYA</italic>
upstream (20 pM)</td>
<td>0.4 μl</td>
<td>Primer PhyADioF (20 pM)</td>
</tr>
<tr><td>0.4 μl</td>
<td>Primer GScpDioR (20 pM)</td>
<td>0.4 μl</td>
<td>Primer <italic>PHYA</italic>
down-stream (20 pM)</td>
<td>0.4 μl</td>
<td>Primer PhyADioR (20 pM)</td>
</tr>
<tr><td>0.7 μl</td>
<td>Water</td>
<td>0.7 μl</td>
<td>Water</td>
<td>0.3 μl</td>
<td>Water</td>
</tr>
<tr><td></td>
<td></td>
<td></td>
<td></td>
<td>0.4 μl</td>
<td>BSA (20 mg/ml)</td>
</tr>
<tr><td>0.5 μl</td>
<td>DNA</td>
<td>0.5 μl</td>
<td>DNA</td>
<td>0.5 μl</td>
<td>PCR product</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn><p>BSA: bovine albumin serum (Thermo Scientific).</p>
</fn>
</table-wrap-foot>
</table-wrap>
<table-wrap id="t0015" position="float"><label>Table 3</label>
<caption><p>PCR conditions.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th colspan="2"><italic>ncpGS</italic>
</th>
<th colspan="2">1st <italic>phyA</italic>
</th>
<th colspan="2">2nd <italic>phyA</italic>
</th>
</tr>
</thead>
<tbody><tr><td>95 °C for 2 min</td>
<td></td>
<td>95 °C for 2 min</td>
<td></td>
<td>95 °C for 2 min</td>
<td></td>
</tr>
<tr><td>95 °C for 30 s</td>
<td></td>
<td>95 °C for 30 s</td>
<td></td>
<td>95 °C for 30 s</td>
<td></td>
</tr>
<tr><td>58 °C for 30 s</td>
<td>35 cycles</td>
<td>52 °C for 30 s</td>
<td>35 cycles</td>
<td>60 °C for 30 s</td>
<td>35 cycles</td>
</tr>
<tr><td>72 °C for 2 min</td>
<td></td>
<td>70 °C for 2 min</td>
<td></td>
<td>72 °C for 1.5 min</td>
<td></td>
</tr>
<tr><td>72 °C for 7 min</td>
<td></td>
<td>70 °C for 7 min</td>
<td></td>
<td>72 °C for 7 min</td>
<td></td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t0020" position="float"><label>Table 4</label>
<caption><p>Primers used in this study.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th>Primer name</th>
<th>Fragment</th>
<th>Sequence (5’-3’)</th>
<th>References</th>
</tr>
</thead>
<tbody><tr><td>GScp839F</td>
<td><italic>ncpGS</italic>
</td>
<td>CACCAATGGGGAGGTTATGC</td>
<td><xref rid="b0410" ref-type="bibr">Yockteng and Nadot (2004)</xref>
</td>
</tr>
<tr><td>GScp1056R</td>
<td><italic>ncpGS</italic>
</td>
<td>CATCTTCCCTCATGCTCTTTGT</td>
<td><xref rid="b0410" ref-type="bibr">Yockteng and Nadot (2004)</xref>
</td>
</tr>
<tr><td>GscpDio1F</td>
<td><italic>ncpGS</italic>
</td>
<td>CCAATGGGGAGGTTATGCCTGGACAG</td>
<td>This study</td>
</tr>
<tr><td>GScpDioR</td>
<td><italic>ncpGS</italic>
</td>
<td>CATCTTCCCTCATGCTCTTTGTACTG</td>
<td>This study</td>
</tr>
<tr><td><italic>PHYA</italic>
upstream</td>
<td><italic>phyA</italic>
</td>
<td>GACTTTGARCCNGTBAAGCCTTAYG</td>
<td><xref rid="b0215" ref-type="bibr">Mathews and Donoghue (1999)</xref>
</td>
</tr>
<tr><td><italic>PHYA</italic>
downstream</td>
<td><italic>phyA</italic>
</td>
<td>GDATDGCRTCCATYTCRTAGTC</td>
<td><xref rid="b0215" ref-type="bibr">Mathews and Donoghue (1999)</xref>
</td>
</tr>
<tr><td>PhyADioF</td>
<td><italic>phyA</italic>
</td>
<td>GTBAAGCCTTAYGAAGTCCCGATGA</td>
<td>This study</td>
</tr>
<tr><td>PhyADioFi</td>
<td><italic>phyA</italic>
</td>
<td>GTCAAYGAGGGGGATGRAGAGGGAG</td>
<td>This study</td>
</tr>
<tr><td>PhyADioR</td>
<td><italic>phyA</italic>
</td>
<td>GCRTCCATYTCRTAGTCCTTCCAAG</td>
<td>This study</td>
</tr>
<tr><td>PhyADioRi</td>
<td><italic>phyA</italic>
</td>
<td>CTGATTYTCCAAYTCTAACTCCTTGTTGAC</td>
<td>This study</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t0025" position="float"><label>Table 5</label>
<caption><p>Data characteristics and statistics from the maximum parsimony analyses of all three individual and the combined data sets.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th></th>
<th>Combined plastid markers</th>
<th><italic>ncpGS</italic>
</th>
<th><italic>phyA</italic>
</th>
<th>Combined data set</th>
</tr>
</thead>
<tbody><tr><td>Total no. of accessions</td>
<td>294</td>
<td>177</td>
<td>177</td>
<td>129</td>
</tr>
<tr><td>No. of outgroup accessions other than Ebenaceae</td>
<td>4</td>
<td>2</td>
<td>2</td>
<td>1</td>
</tr>
<tr><td>No. of outgroup accessions from Ebenaceae</td>
<td>21</td>
<td>2</td>
<td>2</td>
<td>2</td>
</tr>
<tr><td>No. of <italic>Diospyros</italic>
accessions</td>
<td>269</td>
<td>173</td>
<td>173</td>
<td>126</td>
</tr>
<tr><td>No. of <italic>Diospyros</italic>
species</td>
<td>149</td>
<td>64</td>
<td>64</td>
<td>64</td>
</tr>
<tr><td>No. of New Caledonian accessions</td>
<td>98</td>
<td>134</td>
<td>134</td>
<td>86</td>
</tr>
<tr><td>No. of New Caledonian species</td>
<td>28</td>
<td>28</td>
<td>28</td>
<td>28</td>
</tr>
<tr><td>No. of New Caledonian neoendemic accessions</td>
<td>83</td>
<td>112</td>
<td>112</td>
<td>74</td>
</tr>
<tr><td>No. of New Caledonian neoendemic species</td>
<td>21</td>
<td>21</td>
<td>21</td>
<td>21</td>
</tr>
<tr><td>Length of alignment</td>
<td>6556</td>
<td>1039</td>
<td>1187</td>
<td>8542</td>
</tr>
<tr><td>No. of variable characters</td>
<td>1880</td>
<td>532</td>
<td>374</td>
<td>1845</td>
</tr>
<tr><td>No. of parsimony informative characters</td>
<td>1126 (17.2%)</td>
<td>341 (32.8%)</td>
<td>223 (18.8%)</td>
<td>863 (10%)</td>
</tr>
<tr><td>No. of parsimony informative characters NCnc</td>
<td>44 (0.7%)</td>
<td>28 (2.7%)</td>
<td>14 (1.2%)</td>
<td>79 (0.9%)</td>
</tr>
<tr><td>Tree length of best parsimony tree (steps)</td>
<td>3808</td>
<td>1171</td>
<td>689</td>
<td>3259</td>
</tr>
<tr><td>Trees saved (parsimony analysis)</td>
<td>210</td>
<td>4810</td>
<td>1870</td>
<td>930</td>
</tr>
<tr><td>Consistency index</td>
<td>0.603</td>
<td>0.663</td>
<td>0.685</td>
<td>0.692</td>
</tr>
<tr><td>Retention index</td>
<td>0.857</td>
<td>0.857</td>
<td>0.893</td>
<td>0.848</td>
</tr>
<tr><td>Best fitting model</td>
<td>GTR + Γ + I</td>
<td>GTR + Γ</td>
<td>HKY + Γ + I</td>
<td></td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t0030" position="float"><label>Table 6</label>
<caption><p>Genome size of <italic>Diospyros</italic>
and other genera from Ebenoideae. S.D.: standard deviation, N: number of measurements (replicates), <italic>S.p.</italic>
: <italic>Solanum pseudocapsicum</italic>
, <italic>P.s.</italic>
: <italic>Pisum sativum</italic>
‘Kleine Rheinländerin’.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th>Name</th>
<th>Acc. nr</th>
<th>1C-value</th>
<th>S.D.</th>
<th>N</th>
<th>Standard</th>
<th>Material</th>
</tr>
</thead>
<tbody><tr><td><italic>D. calciphila</italic>
</td>
<td>BT313</td>
<td>1.99</td>
<td></td>
<td>1</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. calciphila</italic>
</td>
<td>BT316</td>
<td>1.97</td>
<td></td>
<td>1</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>BT262</td>
<td>1.65</td>
<td>0.0092</td>
<td>5</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. cherrieri</italic>
</td>
<td>BT293</td>
<td>1.57</td>
<td>0.0117</td>
<td>2</td>
<td>S.p.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. discolor</italic>
</td>
<td>EBE100026</td>
<td>0.92</td>
<td>0.0020</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. erudita</italic>
</td>
<td>BT260</td>
<td>2.17</td>
<td></td>
<td>1</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. erudita</italic>
</td>
<td>BT261</td>
<td>2.13</td>
<td>0.0367</td>
<td>3</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. erudita</italic>
</td>
<td>BT280</td>
<td>1.88</td>
<td>0.0253</td>
<td>3</td>
<td>S.p.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT012</td>
<td>1.19</td>
<td>0.0031</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT106</td>
<td>1.13</td>
<td>0.0064</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT144</td>
<td>1.22</td>
<td>0.0032</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT167</td>
<td>1.02</td>
<td>0.0318</td>
<td>4</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT212</td>
<td>1.09</td>
<td>0.0227</td>
<td>2</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. fasciculosa</italic>
</td>
<td>BT335</td>
<td>1.14</td>
<td>0.0300</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. glans</italic>
</td>
<td>BT019</td>
<td>2.03</td>
<td></td>
<td>1</td>
<td>S.p.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. glans</italic>
</td>
<td>BT093</td>
<td>2.02</td>
<td>0.0153</td>
<td>3</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. impolita</italic>
</td>
<td>BT101</td>
<td>1.79</td>
<td></td>
<td>1</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. impolita</italic>
</td>
<td>BT105</td>
<td>1.90</td>
<td>0.0132</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. inconstans</italic>
</td>
<td></td>
<td>1.13</td>
<td>0.0019</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. inexplorata</italic>
</td>
<td>BT304</td>
<td>1.94</td>
<td>0.0693</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. kaki</italic>
</td>
<td>Sharon</td>
<td>2.29</td>
<td>0.0121</td>
<td>3</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. lotus</italic>
</td>
<td>EBE</td>
<td>0.87</td>
<td>0.0075</td>
<td>8</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. lotus</italic>
</td>
<td>EBE03002</td>
<td>0.86</td>
<td>0.0012</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. mespiliformis</italic>
</td>
<td>EBE000001</td>
<td>1.24</td>
<td>0.0029</td>
<td>3</td>
<td>P.s.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. mespiliformis</italic>
</td>
<td>EBE100027</td>
<td>1.27</td>
<td>0.0035</td>
<td>3</td>
<td>P.s.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. minimifolia</italic>
</td>
<td>BT230</td>
<td>1.57</td>
<td>0.0445</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT001</td>
<td>0.82</td>
<td>0.0062</td>
<td>3</td>
<td>S.p.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT036</td>
<td>0.87</td>
<td>0.0475</td>
<td>3</td>
<td>S.p.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT096</td>
<td>0.86</td>
<td>0.0042</td>
<td>3</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. olen</italic>
</td>
<td>BT186</td>
<td>0.90</td>
<td>0.0041</td>
<td>3</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. pancheri</italic>
</td>
<td>BT077</td>
<td>2.28</td>
<td>0.0129</td>
<td>3</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. parviflora</italic>
</td>
<td>BT085</td>
<td>2.16</td>
<td>0.0493</td>
<td>3</td>
<td>P.s.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. pentamera</italic>
</td>
<td>EBE030020</td>
<td>1.97</td>
<td>0.0020</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. perplexa</italic>
</td>
<td>BT002</td>
<td>2.27</td>
<td></td>
<td>1</td>
<td>S.p.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. pustulata</italic>
</td>
<td>BT137</td>
<td>1.54</td>
<td>0.0490</td>
<td>2</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. revolutissima</italic>
</td>
<td>BT222</td>
<td>2.05</td>
<td>0.0148</td>
<td>4</td>
<td>P.s.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. texana</italic>
</td>
<td>EBE020015</td>
<td>0.89</td>
<td>0.8849</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. texana</italic>
</td>
<td>EBE100029</td>
<td>0.89</td>
<td>0.0019</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>D. tridentata</italic>
</td>
<td>BT205</td>
<td>2.21</td>
<td>0.0246</td>
<td>2</td>
<td>S.p.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. tridentata</italic>
</td>
<td>BT206</td>
<td>2.09</td>
<td></td>
<td>1</td>
<td>P.s.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT171</td>
<td>1.61</td>
<td></td>
<td>1</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. umbrosa</italic>
</td>
<td>BT247</td>
<td>1.51</td>
<td>0.0894</td>
<td>3</td>
<td>P.s.</td>
<td>Silicagel</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT100</td>
<td>1.55</td>
<td></td>
<td>1</td>
<td>P.s.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. vieillardii</italic>
</td>
<td>BT216</td>
<td>1.57</td>
<td>0.0238</td>
<td>3</td>
<td>P.s.</td>
<td>Dry</td>
</tr>
<tr><td><italic>D. yatesiana</italic>
</td>
<td></td>
<td>0.60</td>
<td>0.0010</td>
<td>5</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>E. divinorum</italic>
</td>
<td>EBE000002</td>
<td>1.98</td>
<td>0.0014</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>E. undulata</italic>
</td>
<td>EBE100002</td>
<td>0.74</td>
<td>0.0220</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>R. whyteana</italic>
</td>
<td>EBE030021</td>
<td>0.79</td>
<td>0.0031</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
<tr><td><italic>R. whyteana</italic>
</td>
<td>EBE030022</td>
<td>0.78</td>
<td>0.0007</td>
<td>3</td>
<td>S.p.</td>
<td>Fresh</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t0035" position="float"><label>Table 7</label>
<caption><p>Main habitats of New Caledonian neoendemic <italic>Diospyros</italic>
species.</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th colspan="2">Habitat</th>
<th>Species</th>
</tr>
</thead>
<tbody><tr><td colspan="2">Maquis on ultramafic substrates</td>
<td>D<italic>. erudita</italic>
, <italic>D. pancheri</italic>
, <italic>D. parviflora</italic>
, <italic>D. tireliae</italic>
, <italic>D. vieillardii</italic>
</td>
</tr>
<tr><td colspan="2">Dry forests on non-ultramafic substrates</td>
<td><italic>D. cherrieri</italic>
, <italic>D. perplexa</italic>
, <italic>D. yahouensis</italic>
</td>
</tr>
<tr><td colspan="3">
</td>
</tr>
<tr><td rowspan="2">Humid forests at low elevations</td>
<td>Ultramafic substrates</td>
<td><italic>D. pancheri</italic>
, <italic>D. parvilfora</italic>
, <italic>D. umbrosa</italic>
</td>
</tr>
<tr><td>Calcareous rocks</td>
<td><italic>D. tridentata</italic>
</td>
</tr>
<tr><td colspan="2">Humid mountain forests on schist</td>
<td><italic>D. flavocarpa</italic>
, <italic>D. labillardierei</italic>
, <italic>D. trisulca</italic>
</td>
</tr>
<tr><td colspan="3">
</td>
</tr>
<tr><td rowspan="4">Dry coastal forests</td>
<td>Black clays</td>
<td><italic>D. veillonii</italic>
, <italic>D. minimifolia</italic>
, <italic>D. pustulata</italic>
(the latter two can also occur on calcareous substrates)</td>
</tr>
<tr><td>Schist</td>
<td><italic>D. impolita</italic>
</td>
</tr>
<tr><td>Ultramafic substrates</td>
<td><italic>D. revolutissima</italic>
</td>
</tr>
<tr><td>Various substrates</td>
<td><italic>D. pancheri</italic>
</td>
</tr>
<tr><td colspan="2">Coastal forests on coralline substrates</td>
<td><italic>D. calciphila</italic>
, <italic>D. impolita</italic>
</td>
</tr>
<tr><td colspan="2">Humid forests on the east coast</td>
<td><italic>D. glans</italic>
</td>
</tr>
</tbody>
</table>
</table-wrap>
</floats-group>
</pmc>
</record>
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