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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Progressive replacement of embryo-derived cardiac macrophages with age</title><author><name sortKey="Molawi, Kaaweh" sort="Molawi, Kaaweh" uniqKey="Molawi K" first="Kaaweh" last="Molawi">Kaaweh Molawi</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Strasse 10, 13125 Berlin, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Wolf, Yochai" sort="Wolf, Yochai" uniqKey="Wolf Y" first="Yochai" last="Wolf">Yochai Wolf</name><affiliation><nlm:aff id="aff5"><institution>Department of Immunology, The Weizmann Institute of Science, 7610001 Rehovot, Israel</institution></nlm:aff></affiliation></author><author><name sortKey="Kandalla, Prashanth K" sort="Kandalla, Prashanth K" uniqKey="Kandalla P" first="Prashanth K." last="Kandalla">Prashanth K. Kandalla</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Favret, Jeremy" sort="Favret, Jeremy" uniqKey="Favret J" first="Jeremy" last="Favret">Jeremy Favret</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Hagemeyer, Nora" sort="Hagemeyer, Nora" uniqKey="Hagemeyer N" first="Nora" last="Hagemeyer">Nora Hagemeyer</name><affiliation><nlm:aff wicri:cut=" and" id="aff6"><institution>Institute of Neuropathology</institution></nlm:aff></affiliation></author><author><name sortKey="Frenzel, Kathrin" sort="Frenzel, Kathrin" uniqKey="Frenzel K" first="Kathrin" last="Frenzel">Kathrin Frenzel</name><affiliation><nlm:aff wicri:cut=" and" id="aff6"><institution>Institute of Neuropathology</institution></nlm:aff></affiliation></author><author><name sortKey="Pinto, Alexander R" sort="Pinto, Alexander R" uniqKey="Pinto A" first="Alexander R." last="Pinto">Alexander R. Pinto</name><affiliation><nlm:aff id="aff7"><institution>Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton 3800, Victoria, Australia</institution></nlm:aff></affiliation></author><author><name sortKey="Klapproth, Kay" sort="Klapproth, Kay" uniqKey="Klapproth K" first="Kay" last="Klapproth">Kay Klapproth</name><affiliation><nlm:aff id="aff8"><institution>Division of Cellular Immunology, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Henri, Sandrine" sort="Henri, Sandrine" uniqKey="Henri S" first="Sandrine" last="Henri">Sandrine Henri</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Malissen, Bernard" sort="Malissen, Bernard" uniqKey="Malissen B" first="Bernard" last="Malissen">Bernard Malissen</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Rodewald, Hans Reimer" sort="Rodewald, Hans Reimer" uniqKey="Rodewald H" first="Hans-Reimer" last="Rodewald">Hans-Reimer Rodewald</name><affiliation><nlm:aff id="aff8"><institution>Division of Cellular Immunology, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Rosenthal, Nadia A" sort="Rosenthal, Nadia A" uniqKey="Rosenthal N" first="Nadia A." last="Rosenthal">Nadia A. Rosenthal</name><affiliation><nlm:aff id="aff7"><institution>Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton 3800, Victoria, Australia</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff9"><institution>National Heart and Lung Institute, Imperial College London, London SW7 2AZ, England, UK</institution></nlm:aff></affiliation></author><author><name sortKey="Bajenoff, Marc" sort="Bajenoff, Marc" uniqKey="Bajenoff M" first="Marc" last="Bajenoff">Marc Bajenoff</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Prinz, Marco" sort="Prinz, Marco" uniqKey="Prinz M" first="Marco" last="Prinz">Marco Prinz</name><affiliation><nlm:aff wicri:cut=" and" id="aff6"><institution>Institute of Neuropathology</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff6"><institution>BIOSS Centre for Biological Signaling Studies, University of Freiburg, 79106 Freiburg, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Jung, Steffen" sort="Jung, Steffen" uniqKey="Jung S" first="Steffen" last="Jung">Steffen Jung</name><affiliation><nlm:aff id="aff5"><institution>Department of Immunology, The Weizmann Institute of Science, 7610001 Rehovot, Israel</institution></nlm:aff></affiliation></author><author><name sortKey="Sieweke, Michael H" sort="Sieweke, Michael H" uniqKey="Sieweke M" first="Michael H." last="Sieweke">Michael H. Sieweke</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Strasse 10, 13125 Berlin, Germany</institution></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25245760</idno><idno type="pmc">4203946</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203946</idno><idno type="RBID">PMC:4203946</idno><idno type="doi">10.1084/jem.20140639</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">002660</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002660</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Progressive replacement of embryo-derived cardiac macrophages with age</title><author><name sortKey="Molawi, Kaaweh" sort="Molawi, Kaaweh" uniqKey="Molawi K" first="Kaaweh" last="Molawi">Kaaweh Molawi</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Strasse 10, 13125 Berlin, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Wolf, Yochai" sort="Wolf, Yochai" uniqKey="Wolf Y" first="Yochai" last="Wolf">Yochai Wolf</name><affiliation><nlm:aff id="aff5"><institution>Department of Immunology, The Weizmann Institute of Science, 7610001 Rehovot, Israel</institution></nlm:aff></affiliation></author><author><name sortKey="Kandalla, Prashanth K" sort="Kandalla, Prashanth K" uniqKey="Kandalla P" first="Prashanth K." last="Kandalla">Prashanth K. Kandalla</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Favret, Jeremy" sort="Favret, Jeremy" uniqKey="Favret J" first="Jeremy" last="Favret">Jeremy Favret</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Hagemeyer, Nora" sort="Hagemeyer, Nora" uniqKey="Hagemeyer N" first="Nora" last="Hagemeyer">Nora Hagemeyer</name><affiliation><nlm:aff wicri:cut=" and" id="aff6"><institution>Institute of Neuropathology</institution></nlm:aff></affiliation></author><author><name sortKey="Frenzel, Kathrin" sort="Frenzel, Kathrin" uniqKey="Frenzel K" first="Kathrin" last="Frenzel">Kathrin Frenzel</name><affiliation><nlm:aff wicri:cut=" and" id="aff6"><institution>Institute of Neuropathology</institution></nlm:aff></affiliation></author><author><name sortKey="Pinto, Alexander R" sort="Pinto, Alexander R" uniqKey="Pinto A" first="Alexander R." last="Pinto">Alexander R. Pinto</name><affiliation><nlm:aff id="aff7"><institution>Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton 3800, Victoria, Australia</institution></nlm:aff></affiliation></author><author><name sortKey="Klapproth, Kay" sort="Klapproth, Kay" uniqKey="Klapproth K" first="Kay" last="Klapproth">Kay Klapproth</name><affiliation><nlm:aff id="aff8"><institution>Division of Cellular Immunology, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Henri, Sandrine" sort="Henri, Sandrine" uniqKey="Henri S" first="Sandrine" last="Henri">Sandrine Henri</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Malissen, Bernard" sort="Malissen, Bernard" uniqKey="Malissen B" first="Bernard" last="Malissen">Bernard Malissen</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Rodewald, Hans Reimer" sort="Rodewald, Hans Reimer" uniqKey="Rodewald H" first="Hans-Reimer" last="Rodewald">Hans-Reimer Rodewald</name><affiliation><nlm:aff id="aff8"><institution>Division of Cellular Immunology, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Rosenthal, Nadia A" sort="Rosenthal, Nadia A" uniqKey="Rosenthal N" first="Nadia A." last="Rosenthal">Nadia A. Rosenthal</name><affiliation><nlm:aff id="aff7"><institution>Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton 3800, Victoria, Australia</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff9"><institution>National Heart and Lung Institute, Imperial College London, London SW7 2AZ, England, UK</institution></nlm:aff></affiliation></author><author><name sortKey="Bajenoff, Marc" sort="Bajenoff, Marc" uniqKey="Bajenoff M" first="Marc" last="Bajenoff">Marc Bajenoff</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation></author><author><name sortKey="Prinz, Marco" sort="Prinz, Marco" uniqKey="Prinz M" first="Marco" last="Prinz">Marco Prinz</name><affiliation><nlm:aff wicri:cut=" and" id="aff6"><institution>Institute of Neuropathology</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff6"><institution>BIOSS Centre for Biological Signaling Studies, University of Freiburg, 79106 Freiburg, Germany</institution></nlm:aff></affiliation></author><author><name sortKey="Jung, Steffen" sort="Jung, Steffen" uniqKey="Jung S" first="Steffen" last="Jung">Steffen Jung</name><affiliation><nlm:aff id="aff5"><institution>Department of Immunology, The Weizmann Institute of Science, 7610001 Rehovot, Israel</institution></nlm:aff></affiliation></author><author><name sortKey="Sieweke, Michael H" sort="Sieweke, Michael H" uniqKey="Sieweke M" first="Michael H." last="Sieweke">Michael H. Sieweke</name><affiliation><nlm:aff id="aff1"><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Strasse 10, 13125 Berlin, Germany</institution></nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Experimental Medicine</title><idno type="ISSN">0022-1007</idno><idno type="eISSN">1540-9538</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Molawi et al. examine the origin and cellular dynamics of macrophages in the heart during postnatal development. Cardiac macrophages derived from CX3CR1<sup>+</sup> embryonic progenitors persist into adulthood, but the contribution of these cells to resident macrophages declines after birth with diminished self-renewal as the mice age. Over time, the heart is progressively reconstituted with bone marrow–derived macrophages, even in the absence of inflammation.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Exp Med</journal-id><journal-id journal-id-type="iso-abbrev">J. Exp. Med</journal-id><journal-id journal-id-type="hwp">jem</journal-id><journal-id journal-id-type="publisher-id">jem</journal-id><journal-title-group><journal-title>The Journal of Experimental Medicine</journal-title></journal-title-group><issn pub-type="ppub">0022-1007</issn><issn pub-type="epub">1540-9538</issn><publisher><publisher-name>The Rockefeller University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25245760</article-id><article-id pub-id-type="pmc">4203946</article-id><article-id pub-id-type="publisher-id">20140639</article-id><article-id pub-id-type="doi">10.1084/jem.20140639</article-id><article-categories><subj-group subj-group-type="heading"><subject>Brief Definitive Report</subject></subj-group></article-categories><title-group><article-title>Progressive replacement of embryo-derived cardiac macrophages with age</article-title><alt-title alt-title-type="short">Replacement of embryonic cardiac macrophages with age</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Molawi</surname><given-names>Kaaweh</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Wolf</surname><given-names>Yochai</given-names></name><xref ref-type="aff" rid="aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Kandalla</surname><given-names>Prashanth K.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Favret</surname><given-names>Jeremy</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Hagemeyer</surname><given-names>Nora</given-names></name><xref ref-type="aff" rid="aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Frenzel</surname><given-names>Kathrin</given-names></name><xref ref-type="aff" rid="aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Pinto</surname><given-names>Alexander R.</given-names></name><xref ref-type="aff" rid="aff7">8</xref></contrib><contrib contrib-type="author"><name><surname>Klapproth</surname><given-names>Kay</given-names></name><xref ref-type="aff" rid="aff8">9</xref></contrib><contrib contrib-type="author"><name><surname>Henri</surname><given-names>Sandrine</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Malissen</surname><given-names>Bernard</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Rodewald</surname><given-names>Hans-Reimer</given-names></name><xref ref-type="aff" rid="aff8">9</xref></contrib><contrib contrib-type="author"><name><surname>Rosenthal</surname><given-names>Nadia A.</given-names></name><xref ref-type="aff" rid="aff7">8</xref><xref ref-type="aff" rid="aff9">10</xref></contrib><contrib contrib-type="author"><name><surname>Bajenoff</surname><given-names>Marc</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Prinz</surname><given-names>Marco</given-names></name><xref ref-type="aff" rid="aff6">6</xref><xref ref-type="aff" rid="aff6">7</xref></contrib><contrib contrib-type="author"><name><surname>Jung</surname><given-names>Steffen</given-names></name><xref ref-type="aff" rid="aff5">5</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Sieweke</surname><given-names>Michael H.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="aff" rid="aff4">4</xref></contrib></contrib-group><aff id="aff1"><label>1</label><institution>Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille Université, UM2, 13288 Marseille, France</institution></aff><aff id="aff2"><label>2</label><institution>Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, 13288 Marseille, France</institution></aff><aff id="aff3"><label>3</label><institution>Centre National de la Recherche Scientifique (CNRS), UMR7280, 13288 Marseille, France</institution></aff><aff id="aff4"><label>4</label><institution>Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Strasse 10, 13125 Berlin, Germany</institution></aff><aff id="aff5"><label>5</label><institution>Department of Immunology, The Weizmann Institute of Science, 7610001 Rehovot, Israel</institution></aff><aff id="aff6"><label>6</label><institution>Institute of Neuropathology</institution> and<label>7</label><institution>BIOSS Centre for Biological Signaling Studies, University of Freiburg, 79106 Freiburg, Germany</institution></aff><aff id="aff7"><label>8</label><institution>Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton 3800, Victoria, Australia</institution></aff><aff id="aff8"><label>9</label><institution>Division of Cellular Immunology, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany</institution></aff><aff id="aff9"><label>10</label><institution>National Heart and Lung Institute, Imperial College London, London SW7 2AZ, England, UK</institution></aff><author-notes><corresp>CORRESPONDENCE Michael H. Sieweke: <email>sieweke@ciml.univ-mrs.fr</email></corresp><fn><p>K. Molawi and Y. Wolf contributed equally to this paper.</p></fn><fn><p>Steffen Jung and M.H. Sieweke contributed equally to this paper.</p></fn></author-notes><pub-date pub-type="ppub"><day>20</day><month>10</month><year>2014</year></pub-date><volume>211</volume><issue>11</issue><fpage>2151</fpage><lpage>2158</lpage><history><date date-type="received"><day>6</day><month>4</month><year>2014</year></date><date date-type="accepted"><day>19</day><month>8</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 Molawi et al.</copyright-statement><copyright-year>2014</copyright-year><license license-type="openaccess"><license-p>This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see <ext-link ext-link-type="uri" xlink:href="http://www.rupress.org/terms">http://www.rupress.org/terms</ext-link>). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">http://creativecommons.org/licenses/by-nc-sa/3.0/</ext-link>).</license-p></license></permissions><self-uri xlink:role="icon" xlink:href="JEM_20140639_thumb.gif"></self-uri><abstract abstract-type="precis"><p>Molawi et al. examine the origin and cellular dynamics of macrophages in the heart during postnatal development. Cardiac macrophages derived from CX3CR1<sup>+</sup> embryonic progenitors persist into adulthood, but the contribution of these cells to resident macrophages declines after birth with diminished self-renewal as the mice age. Over time, the heart is progressively reconstituted with bone marrow–derived macrophages, even in the absence of inflammation.</p></abstract><abstract><p>Cardiac macrophages (cMΦ) are critical for early postnatal heart regeneration and fibrotic repair in the adult heart, but their origins and cellular dynamics during postnatal development have not been well characterized. Tissue macrophages can be derived from embryonic progenitors or from monocytes during inflammation. We report that within the first weeks after birth, the embryo-derived population of resident CX3CR1<sup>+</sup> cMΦ diversifies into MHCII<sup>+</sup> and MHCII<sup>−</sup> cells. Genetic fate mapping demonstrated that cMΦ derived from CX3CR1<sup>+</sup> embryonic progenitors persisted into adulthood but the initially high contribution to resident cMΦ declined after birth. Consistent with this, the early significant proliferation rate of resident cMΦ decreased with age upon diversification into subpopulations. Bone marrow (BM) reconstitution experiments showed monocyte-dependent quantitative replacement of all cMΦ populations. Furthermore, parabiotic mice and BM chimeras of nonirradiated recipient mice revealed a slow but significant donor contribution to cMΦ. Together, our observations indicate that in the heart, embryo-derived cMΦ show declining self-renewal with age and are progressively substituted by monocyte-derived macrophages, even in the absence of inflammation.</p></abstract></article-meta></front><body><p>Cardiovascular disease represents a leading cause of death in the developed world, and many pathologies result from insufficient repair of cardiac injury. Mammalian wound healing mechanisms in the adult heart involve scar formation, but for a short time window after birth the neonatal heart maintains full regeneration capacity of cardiac tissue (<xref rid="bib20" ref-type="bibr">Porrello et al., 2011</xref>), a process which requires macrophages (<xref rid="bib2" ref-type="bibr">Aurora et al., 2014</xref>). Studies of cardiac repair after myocardial infarction in the adult have highlighted the critical role of infiltrating monocytes and monocyte-derived macrophages for the healing process (<xref rid="bib18" ref-type="bibr">Nahrendorf et al., 2007</xref>; <xref rid="bib24" ref-type="bibr">Swirski et al., 2009</xref>). The focus of these studies has been on monocyte-derived cells, consistent with the traditional view that macrophages are part of the mononuclear phagocyte system and monocyte-derived (<xref rid="bib26" ref-type="bibr">van Furth and Cohn, 1968</xref>; <xref rid="bib8" ref-type="bibr">Geissmann et al., 2010</xref>). Recent evidence, however, suggests that monocyte contribution to macrophages only represents an emergency pathway, as many tissue macrophage populations get seeded in the developing embryo and can self-maintain without major monocyte contribution (<xref rid="bib4" ref-type="bibr">Chorro et al., 2009</xref>; <xref rid="bib10" ref-type="bibr">Ginhoux et al., 2010</xref>; <xref rid="bib13" ref-type="bibr">Hoeffel et al., 2012</xref>; <xref rid="bib21" ref-type="bibr">Schulz et al., 2012</xref>; <xref rid="bib12" ref-type="bibr">Hashimoto et al., 2013</xref>; <xref rid="bib28" ref-type="bibr">Yona et al., 2013</xref>). Macrophages can massively expand in the tissue by local proliferation in response to challenge (<xref rid="bib14" ref-type="bibr">Jenkins et al., 2011</xref>; <xref rid="bib12" ref-type="bibr">Hashimoto et al., 2013</xref>; <xref rid="bib23" ref-type="bibr">Sieweke and Allen, 2013</xref>) and can extensively self-renew without loss of differentiated function in culture upon inactivation of MafB and cMaf transcription factors (<xref rid="bib3" ref-type="bibr">Aziz et al., 2009</xref>). This has led to the proposition that tissue macrophages may have a long-term self-renewal capacity akin to that of stem cells (<xref rid="bib23" ref-type="bibr">Sieweke and Allen, 2013</xref>).</p><p>Examples of tissue macrophages that are independent from monocytes are microglia in the brain and epidermal Langerhans cells (<xref rid="bib1" ref-type="bibr">Ajami et al., 2007</xref>; <xref rid="bib4" ref-type="bibr">Chorro et al., 2009</xref>; <xref rid="bib10" ref-type="bibr">Ginhoux et al., 2010</xref>; <xref rid="bib13" ref-type="bibr">Hoeffel et al., 2012</xref>). In contrast, intestinal or dermal macrophages have a high turnover rate and are constantly replaced from Ly6C<sup>+</sup> blood monocytes (<xref rid="bib29" ref-type="bibr">Zigmond et al., 2012</xref>; <xref rid="bib25" ref-type="bibr">Tamoutounour et al., 2013</xref>). Thus, origin and turnover of tissue macrophages are highly tissue specific and need to be assessed individually for different organ systems (<xref rid="bib23" ref-type="bibr">Sieweke and Allen, 2013</xref>).</p><p>Resident macrophages can be found in all tissues, where they fulfill a variety of tissue-specific functions contributing to homeostasis, development, and regeneration (<xref rid="bib5" ref-type="bibr">Davies et al., 2013</xref>), making them prominent candidates for therapeutic intervention. This holds in particular for the heart, where tissue-resident cardiac macrophages (cMΦ) have been described as CX3CR1<sup>+</sup> cells that can be found throughout the myocardium (<xref rid="bib19" ref-type="bibr">Pinto et al., 2012</xref>). A recent study identified several cMΦ populations, including short-lived monocyte-derived cells that resemble monocyte-derived DCs as well as tissue-resident cMΦ, which were suggested to be primarily of embryonic origin and to self-maintain under homeostatic conditions (<xref rid="bib6" ref-type="bibr">Epelman et al., 2014</xref>).</p><p>Here, we examined the origin and turnover of tissue-resident cMΦ during postnatal development. Using genetic lineage tracing, parabiotic mice, and unconditioned BM chimeras, we demonstrate that embryo-derived cMΦ are gradually replaced by monocyte-derived macrophages with age and in the absence of inflammation or injury. This replacement is mirrored by dynamic changes in the resident cMΦ population composition and decreasing cMΦ self-renewal over time.</p><sec><title>RESULTS AND DISCUSSION</title><p>We focused on the resident cMΦ population and applied a rigorous flow cytometry gating strategy to exclude potential infiltrating leukocytes (<ext-link ext-link-type="uri" xlink:href="http://www.jem.org/cgi/content/full/jem.20140639/DC1" id="supp1">Fig. S1, A–C</ext-link>). Tissue-resident macrophages were identified as positive for the core macrophage signature markers CD14, CD64, and MerTK (<xref rid="bib7" ref-type="bibr">Gautier et al., 2012</xref>; <xref rid="bib25" ref-type="bibr">Tamoutounour et al., 2013</xref>) and the classical macrophage marker F4/80 (<xref ref-type="fig" rid="fig1">Fig. 1 A</xref>). The chemokine receptor CX3CR1 is widely expressed in the mononuclear phagocyte system (<xref rid="bib15" ref-type="bibr">Jung et al., 2000</xref>) and cMΦ have been reported to be CX3CR1-positive (<xref rid="bib19" ref-type="bibr">Pinto et al., 2012</xref>). Therefore, we refined our analysis of the global cMΦ population by testing CX3CR1 expression in <italic>CX3CR1<sup>GFP/+</sup></italic> knock-in mice (<xref rid="bib15" ref-type="bibr">Jung et al., 2000</xref>). Additionally, we included MHCII in our analysis, which can be differentially expressed on tissue macrophages (<xref rid="bib25" ref-type="bibr">Tamoutounour et al., 2013</xref>). We found that in 8-wk-old mice, the majority of cMΦ was CX3CR1<sup>+</sup> (∼80%) and MHCII<sup>+</sup> (∼70%), allowing the delineation of four distinct cMΦ subpopulations (<xref ref-type="fig" rid="fig1">Fig. 1, A and B</xref>). Additional populations of CD11c<sup>+</sup>MHCII<sup>+</sup> or Ly6C<sup>+</sup> cells, referred to as cMΦ by others (<xref rid="bib6" ref-type="bibr">Epelman et al., 2014</xref>), were excluded from our analysis (Fig. S1 B), as data from other tissues suggest that these are monocytes and monocyte-derived dendritic cells (<xref rid="bib25" ref-type="bibr">Tamoutounour et al., 2013</xref>). Consistent with this, these cells have been shown to be short-lived and monocyte-derived (<xref rid="bib6" ref-type="bibr">Epelman et al., 2014</xref>).</p><fig id="fig1" orientation="portrait" position="float"><label>Figure 1.</label><caption><p><bold>cMΦ develop into 4 subpopulations after birth.</bold> (A and B) Cytometry analysis of cMΦ from adult <italic>CX3CR1<sup>GFP/+</sup></italic> mice. (A) FACS profiles of cMΦ populations, pregated on living single CD11b<sup>+</sup> cells with low CD11c and Ly6C expression. (B) Mean of total and subpopulations of cMΦ in absolute numbers (left) or as percentage of total (right) as determined by bead-normalized flow cytometry. Error bars represent SEM (<italic>n</italic> = 4). (C and D) Cytometry analysis (C) and mean percentage (D) of cMΦ subpopulations from <italic>CX3CR1<sup>GFP/+</sup></italic> mice of indicated age. Error bars represent SEM (<italic>n</italic> = 3–4). (E) MHCII<sup>−</sup> and MHCII<sup>+</sup> cMΦ from adult <italic>CX3CR1<sup>cre</sup>:R26-yfp</italic> mice (black line) were analyzed for YFP expression and compared with Cre<sup>−</sup> littermate controls (gray area; <italic>n</italic> = 3–4). Data in all panels are representative of at least two independent experiments.</p></caption><graphic xlink:href="JEM_20140639_Fig1"></graphic></fig><p>To investigate the development of the four cMΦ populations, we analyzed CX3CR1 and MHCII expression in cMΦ from newborn to 30-wk-old mice (<xref ref-type="fig" rid="fig1">Fig. 1, C and D</xref>). We observed that almost all embryo-derived cMΦ present at birth were CX3CR1<sup>+</sup>MHCII<sup>−</sup>. This homogenous cMΦ compartment diversified with age into four subpopulations with a progressive increase of MHCII<sup>+</sup> cMΦ and a decrease of CX3CR1<sup>+</sup> cMΦ. Importantly, genetic fate mapping analysis using <italic>CX3CR1<sup>cre</sup></italic> mice crossed to <italic>R26-yfp</italic> reporter mice (<italic>CX3CR1<sup>cre</sup>:R26-yfp</italic>; <xref rid="bib28" ref-type="bibr">Yona et al., 2013</xref>) revealed that all adult cMΦ subpopulations must have developed from a CX3CR1<sup>+</sup> stage (<xref ref-type="fig" rid="fig1">Fig. 1 E</xref>). These results suggested two not mutually exclusive possibilities for cMΦ subpopulation development: persistence and diversification of embryo-derived cMΦ as suggested elsewhere (<xref rid="bib6" ref-type="bibr">Epelman et al., 2014</xref>), or replacement of embryo-derived cMΦ by adult monocyte-derived macrophages.</p><p>To address these alternatives, we first analyzed the persistence of embryo-derived cMΦ by genetic lineage tracing using CX3CR1-driven tamoxifen (TAM)-inducible Cre recombinase (CreERT2) and <italic>R26-yfp</italic> reporter mice (<italic>CX3CR1<sup>creER</sup>:R26-yfp</italic>; <xref ref-type="fig" rid="fig2">Fig. 2 A</xref>; <xref rid="bib28" ref-type="bibr">Yona et al., 2013</xref>). Tamoxifen-induced pulse labeling at E9 permits us to identify cells derived from yolk sac (YS) CX3CR1<sup>+</sup> macrophages before the onset of definitive hematopoiesis. Labeling at E13 cannot distinguish YS- and hematopoietic stem cell (HSC)–derived macrophages, but should increase cMΦ labeling because the heart is colonized by CX3CR1<sup>+</sup> macrophages at this time (<xref rid="bib6" ref-type="bibr">Epelman et al., 2014</xref>). cMΦ labeling was normalized to YFP<sup>+</sup> microglia, which are YS-derived, remain CX3CR1<sup>+</sup> throughout development, self-maintain without contribution of HSC-derived cells (<xref rid="bib10" ref-type="bibr">Ginhoux et al., 2010</xref>; <xref rid="bib16" ref-type="bibr">Kierdorf et al., 2013</xref>), and therefore represent an internal control for maximal CX3CR1 labeling efficiency. E13 labeling revealed that relative cMΦ labeling declined from ∼35% in newborn mice to ∼18% in 6-wk-old mice (<xref ref-type="fig" rid="fig2">Fig. 2 B</xref>), indicating a loss of embryo-derived cMΦ with age. E9 labeling showed that embryo-derived cMΦ were at least partially of YS origin and similarly declined from ∼14% in neonates to ∼4% in 6-wk-old mice (<xref ref-type="fig" rid="fig2">Fig. 2 C</xref>). Interestingly, all embryo-derived YFP<sup>+</sup> cMΦ were MHCII<sup>−</sup> at birth but had partially differentiated into MHCII<sup>+</sup> cMΦ after 6 wk (<xref ref-type="fig" rid="fig2">Fig. 2 D</xref>). Although this demonstrated that both populations can develop from cells of embryonic origin, the relative contribution of embryo-derived YFP<sup>+</sup> cMΦ to MHCII<sup>+</sup> cMΦ was significantly lower than to MHCII<sup>−</sup> cMΦ, (<xref ref-type="fig" rid="fig2">Fig. 2 E</xref>).</p><fig id="fig2" orientation="portrait" position="float"><label>Figure 2.</label><caption><p><bold>Decreased contribution of embryo-derived macrophages to cMΦ with age.</bold> (A) <italic>CX3CR1<sup>creER</sup>:R26-yfp</italic> embryos were treated with TAM on E9 or E13 and analyzed on the day of delivery (P0) or 6 wk after delivery (P42). (B) Percentage of microglia-normalized YFP<sup>+</sup> cMΦ and representative cytometry plots pregated on total cMΦ at P0 and P42 after E13 labeling. Bars show median (<italic>n</italic> = 9–13). ***, P ≤ 0.005, Mann-Whitney test. Data were pooled from two independent experiments. (C) Representative cytometry plot showing YFP<sup>+</sup> within total cMΦ at P42 and percentage of microglia-normalized YFP<sup>+</sup> cMΦ at P0 and P42 after E9 labeling. Bars show median (<italic>n</italic> = 7) *, P ≤ 0.05. Data were pooled from three independent experiments. (D) Representative cytometry plots showing YFP<sup>+</sup> cells within MHCII<sup>−</sup> and MHCII<sup>+</sup> cMΦ at P0 and P42 after E13 labeling (<italic>n</italic> = 9–13). (E) Quantification of YFP<sup>+</sup> cells within MHCII<sup>−</sup> and MHCII<sup>+</sup> cMΦ at P42 after E13 labeling. Bars show the median (<italic>n</italic> = 9–13). ***, P ≤ 0.005, Mann-Whitney test. Data were pooled from two independent experiments. (F) Adult TAM-treated <italic>CX3CR1<sup>creER</sup>:R26-yfp</italic> mice were analyzed for YFP<sup>+</sup> cMΦ and microglia at 1 and 4 wk after treatment. Percentage of microglia-normalized YFP<sup>+</sup> cMΦ is shown as median with extreme samples as error bars (<italic>n</italic> = 3). Representative cytometry plots show YFP<sup>+</sup> within total cMΦ. Unless otherwise indicated data in all panels are representative of two independent experiments.</p></caption><graphic xlink:href="JEM_20140639_Fig2"></graphic></fig><p>We further assessed the turnover of CX3CR1<sup>+</sup> cMΦ in the adult heart by pulse labeling adult <italic>CX3CR1<sup>creER</sup>:R26-yfp</italic> (<xref ref-type="fig" rid="fig2">Fig. 2 F</xref>). 1 wk after treatment, labeling in cMΦ corresponded to ∼60% of microglia labeling but drastically decreased to ∼20% after 4 wk. By comparison, microglia labeling was nearly complete after 1 wk and remained unchanged thereafter (<xref rid="bib11" ref-type="bibr">Goldmann et al., 2013</xref>). Collectively, our lineage tracing experiments argue that a significant proportion of macrophages established in early embryonic development is still present at birth but is gradually lost with age.</p><p>The relatively fast turnover of labeled tissue-resident cMΦ suggested that local self-renewal was insufficient to maintain the resident cMΦ pool. We therefore analyzed the proliferative activity of cMΦ subpopulations in adult mice by BrdU incorporation after a 4-h pulse labeling period and by Ki67 staining (<xref ref-type="fig" rid="fig3">Fig. 3 A</xref>). The overall number of proliferating macrophages in adult hearts was low, particularly in MHCII<sup>+</sup> populations, but the proliferative rate of CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ significantly exceeded that of all other subpopulations. Interestingly, this population was also the only cMΦ subset present at birth but declined with age at the expense of the other cMΦ populations (<xref ref-type="fig" rid="fig1">Fig. 1, C and D</xref>). We therefore analyzed the evolution of cMΦ proliferation with age. Both BrdU incorporation and Ki67 staining showed a high proliferative rate in newborn CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ, which gradually decreased by 8–10-fold both in total cMΦ (<xref ref-type="fig" rid="fig3">Fig. 3 B</xref>) and in the CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ population (<xref ref-type="fig" rid="fig3">Fig. 3 C</xref>). Together, these data suggest that both a successive loss of the cell population with the highest proliferative rate (CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ) and a strong decrease of the proliferation rate collectively result in progressively decreasing self-renewal of the tissue-resident cMΦ pool.</p><fig id="fig3" orientation="portrait" position="float"><label>Figure 3.</label><caption><p><bold>Decreased proliferation rate of embryo-derived cMΦ with age.</bold> (A) cMΦ subpopulations of adult <italic>CX3CR1<sup>GFP/+</sup></italic> mice were analyzed for BrdU incorporation and expression of Ki67 by flow cytometry four hours after BrdU injection (i.p.). Bars show median (<italic>n</italic> = 12–13). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.005; Wilcoxon test. (B and C) Total (B) or CX3CR1<sup>+</sup>MHCII<sup>−</sup> (C) cMΦ of <italic>CX3CR1<sup>GFP/+</sup></italic> mice of indicated age were analyzed for BrdU incorporation and Ki67 expression by flow cytometry 4 h after BrdU injection (i.p.). Bars show median (<italic>n</italic> = 4–15). ***, P ≤ 0.005, Mann-Whitney test. Data in all panels were pooled from 2–3 independent experiments.</p></caption><graphic xlink:href="JEM_20140639_Fig3"></graphic></fig><p>We next addressed the question of how the age-dependent loss of embryo-derived macrophages is compensated. To test the ability of monocytes to contribute to cMΦ populations, we generated BM chimeras using WT hosts and BM from transgenic mice expressing dTomato from a ubiquitously expressed human Ubiquitin-C promoter (Ubow mice; <xref rid="bib9" ref-type="bibr">Ghigo et al., 2013</xref>) and GFP from the CX3CR1 locus that makes it possible to monitor grafted cells in all four cMΦ populations. Graft-derived dTomato<sup>+</sup> cMΦ contributed to all four cMΦ populations from 4 wk after transplantation and almost completely replaced host cMΦ populations within 8 wk (<xref ref-type="fig" rid="fig4">Fig. 4 A</xref>). However, a small radio-resistant population (∼10%) persisted for 36 wk without monocyte contribution. To further analyze whether cMΦ replacement was monocyte-dependent, we generated mixed chimeras with BM from WT (CD45.1) and CCR2<sup>−/−</sup> (CD45.2) CX3CR1<sup>GFP/+</sup> mice in CD45.1/CD45.2 double-positive WT hosts (<xref ref-type="fig" rid="fig4">Fig. 4 B</xref>). CCR2<sup>−/−</sup> mice have reduced numbers of Ly6C<sup>+</sup> monocytes in the blood (<xref rid="bib22" ref-type="bibr">Serbina and Pamer, 2006</xref>) and can therefore be used to analyze the contribution of circulating monocytes and CCR2-dependent progenitors to tissue macrophage populations (<xref rid="bib28" ref-type="bibr">Yona et al., 2013</xref>; <xref rid="bib25" ref-type="bibr">Tamoutounour et al., 2013</xref>). Blood analysis indeed confirmed that the vast majority of monocytes but not B cells were of WT origin. Graft-derived cMΦ were almost completely derived from WT cells (<xref ref-type="fig" rid="fig4">Fig. 4 B</xref>). Collectively these two experiments established that HSC-derived CCR2-dependent cells, most likely Ly6C<sup>+</sup> monocytes, have the capacity to differentiate into all cMΦ subsets. Residual host-derived cMΦ included both MHCII<sup>+</sup> and MHCII<sup>−</sup> cMΦ but showed a lower proportion of MHCII<sup>+</sup> cells than grafted monocyte-derived cMΦ (<xref ref-type="fig" rid="fig4">Fig. 4 B</xref>).</p><fig id="fig4" orientation="portrait" position="float"><label>Figure 4.</label><caption><p><bold>Monocytes contribute to four cMΦ subpopulations in adult mice.</bold> (A) WT mice were lethally irradiated and reconstituted with BM from <italic>Ubow:CX3CR1<sup>GFP/+</sup></italic> mice. cMΦ were analyzed for contribution of dTomato<sup>+</sup> cells at indicated time points after reconstitution and graft-derived cells were analyzed for CX3CR1 and MHCII expression. Data are presented as mean percentage ± SEM (<italic>n</italic> = 3–7) and derived from two independent experiments. (B) Mixed BM chimeras were generated by reconstituting lethally irradiated WT mice (<italic>CD45.1/CD45.2</italic>) with BM from <italic>CCR2<sup>−/−</sup>:CX3CR1<sup>GFP/+</sup></italic> (<italic>CD45.2</italic>) and <italic>WT CX3CR1<sup>GFP/+</sup></italic> (<italic>CD45.1</italic>) mice. cMΦ, circulating CD11b<sup>+</sup>CD115<sup>+</sup> monocytes (Ly6C<sup>+</sup> and Ly6C<sup>−</sup>) and B220<sup>+</sup> B cells were analyzed for WT (CD45.1) and CCR2<sup>−/−</sup> (CD45.2) contribution. Host- and graft-derived cMΦ were analyzed for the ratio of MHCII<sup>+</sup>/MHCII<sup>−</sup> cMΦ. Bars show median (<italic>n</italic> = 4). *, P ≤ 0.05, Mann-Whitney test. (C) Parabiosis was established between adult <italic>CCR2<sup>−/−</sup></italic> (CD45.2) and <italic>WT</italic> (CD45.1) mice. After 10 wk, <italic>CCR2<sup>−/−</sup></italic> mice were analyzed for contribution of CD45.1<sup>+</sup> non-host cells to cMΦ (total, MHCII<sup>+</sup>, and MHCII<sup>−</sup>), circulating monocytes (Ly6C<sup>+</sup> and Ly6C<sup>−</sup>), and B cells. For CD45.1<sup>−</sup> host and CD45.1<sup>+</sup> non-host cMΦ, the ratio of MHCII<sup>+</sup>/MHCII<sup>−</sup> cMΦ was calculated and compared. Bars show median (<italic>n</italic> = 4). **, P ≤ 0.01, Mann-Whitney test. (D) BM chimeras were generated by transfer of LT-HSC isolated from <italic>panYFP</italic> mice into nonirradiated <italic>Rag2<sup>−/−</sup></italic>γ<italic><sub>c</sub><sup>−/−</sup>Kit<sup>W/Wv</sup></italic> mice. cMΦ (total, MHCII<sup>+</sup>, and MHCII<sup>−</sup>) and neutrophils (Gr1<sup>hi</sup> and SSC<sup>hi</sup>) were analyzed for contribution of grafted YFP<sup>+</sup> cells 8 wk after transplantation. Bars show median (<italic>n</italic> = 6). **, P ≤ 0.01, Mann-Whitney test. Data in all panels are representative of two independent experiments.</p></caption><graphic xlink:href="JEM_20140639_Fig4"></graphic></fig><p>To examine monocyte contribution to cMΦ populations under homeostatic conditions, we generated parabiotic couples of WT (CD45.1) and CCR2<sup>−/−</sup> (CD45.2) mice and analyzed non-host contributions after 10 wk of parabiosis in CCR2<sup>−/−</sup> partners (<xref ref-type="fig" rid="fig4">Fig. 4 C</xref>). Circulating B cells were equally distributed between the partner mice. In the CCR2-deficient host Ly6C<sup>+</sup> monocytes had reached ∼70% non-host chimerism. About 16% of total cMΦ in CCR2-deficient mice were of non-host origin. Given a 70% chimerism in Ly6C<sup>+</sup> monocytes, the likely cells of origin, and the possibility that depending on lifetime and exchange rate cMΦ may not be fully equilibrated after 10 wk, these observations indicated a significant monocyte contribution to tissue-resident cMΦ. Again, non-host contribution to MHCII<sup>+</sup> cMΦ (∼30%) was significantly higher compared with MHCII<sup>−</sup> cMΦ (∼5%) as highlighted by MHCII<sup>+</sup>/MHCII<sup>−</sup> cMΦ ratios for non-host and host-derived cells (<xref ref-type="fig" rid="fig4">Fig. 4 C</xref>).</p><p>As a complimentary experimental approach that does not depend on myeloablative conditioning, we used <italic>Rag2<sup>−/−</sup></italic>γ<italic><sub>c</sub><sup>−/−</sup>Kit<sup>W/Wv</sup></italic> mice, which are universal HSC recipients that accept grafts without prior irradiation (<xref rid="bib27" ref-type="bibr">Waskow et al., 2009</xref>), and generated BM chimeras by transplantation of HSC from <italic>panYFP</italic> mice (<xref rid="bib17" ref-type="bibr">Luche et al., 2013</xref>). More than 90% of neutrophils were YFP<sup>+</sup> after 8 wk, demonstrating efficient reconstitution (<xref ref-type="fig" rid="fig4">Fig. 4 D</xref>). Analysis of cMΦ revealed ∼7% YFP<sup>+</sup> cells in the total population. Again, donor cells showed a higher contribution to MHCII<sup>+</sup> (∼8% YFP<sup>+</sup>) than to MHCII<sup>−</sup> cMΦ (∼4% YFP<sup>+</sup>) (<xref ref-type="fig" rid="fig4">Fig. 4 D</xref>). In the same mice, no donor contribution to microglia cells was observed (K. Klapproth and H.R. Rodewald, personal communication). Together, these experiments clearly demonstrated a slow but significant replacement of tissue-resident cMΦ by infiltrating monocyte-derived macrophages, which preferentially contributed to MHCII<sup>+</sup> cMΦ.</p><p>In the present study, we have analyzed homeostasis, origin, and turnover of resident macrophages in the heart during postnatal development. We have shown that the cMΦ compartment is undergoing dynamic changes with age. Embryo-derived macrophages present in newborn mice are all CX3CR1<sup>+</sup>MHCII<sup>−</sup> and at least partially YS-derived. After birth, the cMΦ compartment diversifies into four subpopulations defined by MHCII and CX3CR1 expression. Our results show that this diversification is due both to differentiation of persisting embryo-derived CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ and infiltrating monocyte-derived macrophages, which both can contribute to all four cMΦ subpopulations. However, monocytes preferentially give rise to MHCII<sup>+</sup> cMΦ, whereas embryo-derived or radio-resistant cMΦ had a higher proportion of MHCII<sup>−</sup> cells, explaining the relative increase of MHCII<sup>+</sup> cMΦ and the reduction of the CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ subpopulation with age. The dynamic change of the cMΦ subpopulation distribution therefore appears to reflect the gradual replacement of embryo-derived cMΦ by monocyte-derived macrophages. The increasing contribution of monocyte-derived macrophages to the cMΦ pool with age may be explained by the decreasing self-renewal of embryo-derived cMΦ. Although CX3CR1<sup>+</sup>MHCII<sup>−</sup> cMΦ that contain the majority of embryo-derived macrophages have a higher proliferative rate than the other subpopulations, overall cMΦ self-renewal is decreasing with age and is insufficient to sustain the cMΦ pool without input from infiltrating monocytes. It will be interesting to determine whether the loss of self-renewal capacity in cMΦ is permanent or might be reactivated. The observed mechanism of cMΦ homeostasis differs from other macrophage populations such as microglia, Langerhans cells, or alveolar macrophages, which in the absence of tissue damage or inflammation appear to self-maintain in tissues without replacement from circulating cells. It is also distinct from the high turnover rate of dermal tissue macrophages or intestinal lamina propria macrophages that are constantly replenished from circulating monocytes (<xref rid="bib29" ref-type="bibr">Zigmond et al., 2012</xref>; <xref rid="bib25" ref-type="bibr">Tamoutounour et al., 2013</xref>). In contrast to what was proposed elsewhere (<xref rid="bib6" ref-type="bibr">Epelman et al., 2014</xref>), our results also show that inflammation or injury are not necessarily required for replacement of embryo-derived cardiac tissue macrophages by monocytes. It will be interesting to determine whether dynamic age-dependent changes in resident tissue macrophage populations also occur in other tissues and whether they may be important for the differences in repair mechanisms and regenerative capacity observed between young and old individuals.</p></sec><sec sec-type="materials|methods"><title>MATERIALS AND METHODS</title><sec><title></title><sec><title>Mice.</title><p>CD45.1 and CD45.2 C57BL/6 mice were obtained from Charles River. All transgenic mice used in this study have a C57BL/6 background (>7 backcrosses). <italic>CX3CR1<sup>GFP/+</sup></italic> mice (<xref rid="bib15" ref-type="bibr">Jung et al., 2000</xref>), <italic>CCR2<sup>−/−</sup></italic>,<italic> Rosa-26-yfp</italic>, <italic>CX3CR1<sup>cre</sup></italic> (JAX Stock No. 25524 B6.C-Cx3cr1/J), and <italic>CX3CR1<sup>creER</sup></italic> mice (JAX Stock No. 20940 B6J.129-Cx3cr1/J; <xref rid="bib28" ref-type="bibr">Yona et al., 2013</xref>), <italic>Ubow</italic> mice (<xref rid="bib9" ref-type="bibr">Ghigo et al., 2013</xref>), <italic>Rag2<sup>−/−</sup></italic>γ<italic><sub>c</sub><sup>−/−</sup>Kit<sup>W/Wv</sup></italic> mice (<xref rid="bib27" ref-type="bibr">Waskow et al., 2009</xref>), and <italic>panYFP</italic> mice (<xref rid="bib17" ref-type="bibr">Luche et al., 2013</xref>) were described elsewhere. Experiments were performed on 6–10-wk-old mice, if not stated otherwise. Experiments involving <italic>CX3CR1<sup>cre</sup></italic> and <italic>CX3CR1<sup>creER</sup></italic> mice included littermate controls. For other experiments C57BL/6 mice served as controls. All mouse experiments were performed under specific pathogen-free conditions. Animals were handled according to protocols approved by the Weizmann Institute Animal Care Committee (Israel), the Federal Ministry for Nature, Environment and Consumers’ Protection of the state of Baden-Württemberg (Germany) or the animal ethics committee of Marseille (France) and were performed in accordance to the respective international, national, and institutional regulations.</p> </sec><sec><title>Parabiosis.</title><p>9-wk-old C57BL/6 CD45.1 mice were sutured together with 9-wk-old B6 <italic>CCR2<sup>−/−</sup></italic> CD45.2 and subsequently kept under Bactrim for 10 wk before analysis.</p></sec><sec><title>BM chimeras.</title><p>7–10-wk-old host animals were lethally irradiated and were reconstituted with donor BM by i.v. injection of minimum 10<sup>6</sup> BM cells. Donor BM was isolated from femurs and tibias of donor mice, filtered through a 70-µm mesh and resuspended in PBS for i.v. injection. <italic>Rag2<sup>−/−</sup></italic>γ<italic><sub>c</sub><sup>−/−</sup>Kit<sup>W/Wv</sup></italic> mice were reconstituted with long-term (LT)–HSC from <italic>panYFP</italic> BM without prior conditioning as previously described (<xref rid="bib27" ref-type="bibr">Waskow et al., 2009</xref>). LT-HSCs were defined as lineage-negative (B220<sup>−</sup>, CD3e<sup>−</sup>, CD4<sup>−</sup>, CD8a<sup>−</sup>, CD11b<sup>−</sup>, CD19<sup>−</sup>, Gr1<sup>−</sup>, Ter119<sup>−</sup>, NK1.1<sup>−</sup>), Kit<sup>+</sup>, Sca1<sup>+</sup>, CD48<sup>−</sup>, and CD150<sup>+</sup>.</p></sec><sec><title>Tamoxifen treatment.</title><p>Tamoxifen (TAM; Sigma-Aldrich) was dissolved in 100% ethanol to get a 1 g/ml solution and was 10-fold diluted in corn oil (Sigma-Aldrich) to get the 100 mg/ml final solution for oral or i.p. administration. To induce Cre-mediated gene recombination in 5- to 7-wk-old <italic>CX3CR1<sup>creER</sup></italic> mice, 5 mg TAM was administrated orally for 5 consecutive days (25 mg total). For Cre induction in the embryo, 200 µl of 20 mg/ml TAM and 10 mg/ml Progesterone dissolved in corn oil was injected i.p. into pregnant females at 9 or 13 days post coitum (dpc).</p></sec><sec><title>BrdU pulsing.</title><p>BrdU was purchased from Sigma-Aldrich. Mice were injected with 0.1 mg BrdU/g body weight. Newborn mice were injected subcutaneously, older mice intraperitoneally. cMΦ isolated from BrdU injected mice were analyzed 4 h after injection for BrdU incorporation using the BrdU Flow kit (BD).</p></sec><sec><title>Flow cytometry.</title><p>Cells were acquired on FACSCanto, LSRII, and LSRFortessa systems (BD) and analyzed with FlowJo software (Tree Star). The following antibodies were used for staining cells: anti-CD11b (clone M1/70; eBioscience), anti-CD11c (clone N418; eBioscience), anti-CD14 (clone Sa2-8; eBioscience), anti-F4/80 (clone BM8; eBioscience), anti-MHCII (clone M5/114.15.2; eBioscience), anti-CD45.1 (clone A20; eBioscience), anti-CD45.2 (clone 104; eBioscience), anti-B220 (clone RA3-6B2; eBioscience), anti-CD115 (clone AFS98; eBioscience), anti-CD117 (clone 2B8; eBioscience), anti-Sca1 (clone D7; eBioscience), anti-CD48 (clone JM48-1; eBioscience), anti-Gr1 (clone RB6-8C5; BioLegend), anti-CD64 (clone X54-5/7.1; BioLegend), anti-Ly6G (clone 1A8; BioLegend), anti-CD150 (clone TC15-12F12.2; BioLegend), anti-Ly6C (clone AL21; BD), anti-Ki67 (clone B56; BD), anti-CD4 (clone H129.19; BD), anti-CD8 (clone 53–6.7; BD), anti-CD19 (clone ID3; BD), Ter119 (clone Ter119; BD), NK1.1 (clone PK136; BD), anti-F4/80, anti-MerTK (clone BAF591; R&D Systems), anti-BrdU (clone MoBU-1; Life Technologies). Before staining, cells were preincubated with Fc receptor blocking antibody (clone 2.4 G2; BD). LIVE/DEAD Fixable Aqua Dead Cell Stain kit (Life Technologies) was used to stain dead cells. Absolute cell numbers were determined by using Flow-Count Fluorosperes (Beckman Coulter) according to the manufacturer’s instructions.</p></sec><sec><title>Preparation of tissue samples.</title><p>Peripheral blood leukocytes were isolated by density separation using Lympholite-M solution (Cedarlane) according to manufacturer’s instructions. For preparing single cell suspension from heart and brain, the mice were perfused with PBS before collecting the tissue. For cMΦ, the heart was macerated and incubated with 1 mg/ml collagenase-2 (Worthington Biochemical Corporation) and 0.15 mg/ml DNase1 (Sigma-Aldrich) at 37°C for 30 min during constant agitation. Resulting cell suspension was filtered through a 70-µm mesh and erythrocytes were removed by ACK lysis. For microglia isolation a gradient of 70, 37, 30% Percoll was used. The brain tissue was homogenized and incubated with HBSS solution containing 2% BSA (Sigma-Aldrich), 1 mg/ml collagenase <sc>d</sc> (Roche), and 0.15 mg/ml DNase1, filtered through a 70-µm mesh, and resuspended in 70% Percoll, before density centrifugation (800 <italic>g</italic> for 30 min at 20°C with low acceleration and no brake).</p></sec><sec><title>Online supplemental material.</title><p>Fig. S1 depicts the full gating strategy and population characterization of cMΦ. Online supplemental material is available at <ext-link ext-link-type="uri" xlink:href="http://www.jem.org/cgi/content/full/jem.20140639/DC1">http://www.jem.org/cgi/content/full/jem.20140639/DC1</ext-link>.</p></sec></sec></sec><sec sec-type="supplementary-material"><title>Supplementary Material</title><supplementary-material id="PMC_1" content-type="local-data"><caption><title>Supplemental Material</title></caption><media mimetype="text" mime-subtype="html" xlink:href="supp_211_11_2151__index.html"></media><media xlink:role="associated-file" mimetype="application" mime-subtype="pdf" xlink:href="supp_jem.20140639_JEM_20140639_sm.pdf"></media></supplementary-material></sec></body><back><ack><p>We thank Dr. Sandrine Sarrazin for critical reading of the manuscript and help with statistical analysis, L. Razafindramanana for animal handling, and M. Barad, A. Zouine, and S. Bigot for cytometry support.</p><p>K. Molawi was supported by an HFSP long-term fellowship. The work was supported by grants to M.H. Sieweke from Aviesan ITMO IHP exploratory Project A12194AS and CNRS PICS program No. 5730. M.H. Sieweke is a Fondation pour la Recherche Médicale (DEQ. 20071210559 and DEQ. 20110421320) and INSERM-Helmholtz group leader. The work was further supported by the Israel Science Foundation (ISF), the Deutsche Forschungsgemeinschaft (DFG) Research Unit (FOR) 1336 (S. Jung and M. Prinz), and DFG-SFB 938-project L (H.-R. Rodewald and K. 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