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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <italic>Dermatophagoides pteronyssinus</italic> Extracts</title><author><name sortKey="Casset, Anne" sort="Casset, Anne" uniqKey="Casset A" first="Anne" last="Casset">Anne Casset</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Mari, Adriano" sort="Mari, Adriano" uniqKey="Mari A" first="Adriano" last="Mari">Adriano Mari</name><affiliation><nlm:aff id="A4">Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</nlm:aff></affiliation></author><author><name sortKey="Purohit, Ashok" sort="Purohit, Ashok" uniqKey="Purohit A" first="Ashok" last="Purohit">Ashok Purohit</name><affiliation><nlm:aff id="A5">Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital, Strasbourg, France</nlm:aff></affiliation></author><author><name sortKey="Resch, Yvonne" sort="Resch, Yvonne" uniqKey="Resch Y" first="Yvonne" last="Resch">Yvonne Resch</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Weghofer, Margit" sort="Weghofer, Margit" uniqKey="Weghofer M" first="Margit" last="Weghofer">Margit Weghofer</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Ferrara, Rosetta" sort="Ferrara, Rosetta" uniqKey="Ferrara R" first="Rosetta" last="Ferrara">Rosetta Ferrara</name><affiliation><nlm:aff id="A4">Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</nlm:aff></affiliation></author><author><name sortKey="Thomas, Wayne R" sort="Thomas, Wayne R" uniqKey="Thomas W" first="Wayne R." last="Thomas">Wayne R. Thomas</name><affiliation><nlm:aff id="A6">Center of Child Health Research, University of Western Australia, Telethon Institute for Child Health Research, Perth, W.A., Australia</nlm:aff></affiliation></author><author><name sortKey="Alessandri, Claudia" sort="Alessandri, Claudia" uniqKey="Alessandri C" first="Claudia" last="Alessandri">Claudia Alessandri</name><affiliation><nlm:aff id="A4">Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</nlm:aff></affiliation></author><author><name sortKey="Chen, Kuan Wei" sort="Chen, Kuan Wei" uniqKey="Chen K" first="Kuan-Wei" last="Chen">Kuan-Wei Chen</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="De Blay, Frederic" sort="De Blay, Frederic" uniqKey="De Blay F" first="Frédéric" last="De Blay">Frédéric De Blay</name><affiliation><nlm:aff id="A5">Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital, Strasbourg, France</nlm:aff></affiliation></author><author><name sortKey="Valenta, Rudolf" sort="Valenta, Rudolf" uniqKey="Valenta R" first="Rudolf" last="Valenta">Rudolf Valenta</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Christian Doppler Laboratories for Allergy Research, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Vrtala, Susanne" sort="Vrtala, Susanne" uniqKey="Vrtala S" first="Susanne" last="Vrtala">Susanne Vrtala</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Development of Allergen Chips, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22722650</idno><idno type="pmc">4594775</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594775</idno><idno type="RBID">PMC:4594775</idno><idno type="doi">10.1159/000337654</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">002106</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002106</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <italic>Dermatophagoides pteronyssinus</italic> Extracts</title><author><name sortKey="Casset, Anne" sort="Casset, Anne" uniqKey="Casset A" first="Anne" last="Casset">Anne Casset</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Mari, Adriano" sort="Mari, Adriano" uniqKey="Mari A" first="Adriano" last="Mari">Adriano Mari</name><affiliation><nlm:aff id="A4">Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</nlm:aff></affiliation></author><author><name sortKey="Purohit, Ashok" sort="Purohit, Ashok" uniqKey="Purohit A" first="Ashok" last="Purohit">Ashok Purohit</name><affiliation><nlm:aff id="A5">Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital, Strasbourg, France</nlm:aff></affiliation></author><author><name sortKey="Resch, Yvonne" sort="Resch, Yvonne" uniqKey="Resch Y" first="Yvonne" last="Resch">Yvonne Resch</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Weghofer, Margit" sort="Weghofer, Margit" uniqKey="Weghofer M" first="Margit" last="Weghofer">Margit Weghofer</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Ferrara, Rosetta" sort="Ferrara, Rosetta" uniqKey="Ferrara R" first="Rosetta" last="Ferrara">Rosetta Ferrara</name><affiliation><nlm:aff id="A4">Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</nlm:aff></affiliation></author><author><name sortKey="Thomas, Wayne R" sort="Thomas, Wayne R" uniqKey="Thomas W" first="Wayne R." last="Thomas">Wayne R. Thomas</name><affiliation><nlm:aff id="A6">Center of Child Health Research, University of Western Australia, Telethon Institute for Child Health Research, Perth, W.A., Australia</nlm:aff></affiliation></author><author><name sortKey="Alessandri, Claudia" sort="Alessandri, Claudia" uniqKey="Alessandri C" first="Claudia" last="Alessandri">Claudia Alessandri</name><affiliation><nlm:aff id="A4">Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</nlm:aff></affiliation></author><author><name sortKey="Chen, Kuan Wei" sort="Chen, Kuan Wei" uniqKey="Chen K" first="Kuan-Wei" last="Chen">Kuan-Wei Chen</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="De Blay, Frederic" sort="De Blay, Frederic" uniqKey="De Blay F" first="Frédéric" last="De Blay">Frédéric De Blay</name><affiliation><nlm:aff id="A5">Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital, Strasbourg, France</nlm:aff></affiliation></author><author><name sortKey="Valenta, Rudolf" sort="Valenta, Rudolf" uniqKey="Valenta R" first="Rudolf" last="Valenta">Rudolf Valenta</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Christian Doppler Laboratories for Allergy Research, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author><author><name sortKey="Vrtala, Susanne" sort="Vrtala, Susanne" uniqKey="Vrtala S" first="Susanne" last="Vrtala">Susanne Vrtala</name><affiliation><nlm:aff id="A1">Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Development of Allergen Chips, Medical University of Vienna, Vienna, Austria</nlm:aff></affiliation></author></analytic><series><title level="j">International archives of allergy and immunology</title><idno type="ISSN">1018-2438</idno><idno type="eISSN">1423-0097</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title><p id="P1">Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available <italic>Dermatophagoides pteronyssinus</italic> extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity.</p></sec><sec id="S2"><title>Methods</title><p id="P2">Antibodies specific for several <italic>D. pteronyssinus</italic> allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot.</p></sec><sec id="S3"><title>Results</title><p id="P3">Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0–40.8 μg ml<sup>−1</sup>; Der p 2: 1.7–45.0 μg ml<sup>−1</sup>). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">Commercially available <italic>D. pteronyssinus</italic> extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9211652</journal-id><journal-id journal-id-type="pubmed-jr-id">2196</journal-id><journal-id journal-id-type="nlm-ta">Int Arch Allergy Immunol</journal-id><journal-id journal-id-type="iso-abbrev">Int. Arch. Allergy Immunol.</journal-id><journal-title-group><journal-title>International archives of allergy and immunology</journal-title></journal-title-group><issn pub-type="ppub">1018-2438</issn><issn pub-type="epub">1423-0097</issn></journal-meta><article-meta><article-id pub-id-type="pmid">22722650</article-id><article-id pub-id-type="pmc">4594775</article-id><article-id pub-id-type="doi">10.1159/000337654</article-id><article-id pub-id-type="manuscript">EMS65147</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <italic>Dermatophagoides pteronyssinus</italic> Extracts</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Casset</surname><given-names>Anne</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Mari</surname><given-names>Adriano</given-names></name><xref ref-type="aff" rid="A4">d</xref></contrib><contrib contrib-type="author"><name><surname>Purohit</surname><given-names>Ashok</given-names></name><xref ref-type="aff" rid="A5">e</xref></contrib><contrib contrib-type="author"><name><surname>Resch</surname><given-names>Yvonne</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Weghofer</surname><given-names>Margit</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Ferrara</surname><given-names>Rosetta</given-names></name><xref ref-type="aff" rid="A4">d</xref></contrib><contrib contrib-type="author"><name><surname>Thomas</surname><given-names>Wayne R.</given-names></name><xref ref-type="aff" rid="A6">f</xref></contrib><contrib contrib-type="author"><name><surname>Alessandri</surname><given-names>Claudia</given-names></name><xref ref-type="aff" rid="A4">d</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Kuan-Wei</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>de Blay</surname><given-names>Frédéric</given-names></name><xref ref-type="aff" rid="A5">e</xref></contrib><contrib contrib-type="author"><name><surname>Valenta</surname><given-names>Rudolf</given-names></name><xref ref-type="aff" rid="A1">a</xref><xref ref-type="aff" rid="A2">b</xref></contrib><contrib contrib-type="author"><name><surname>Vrtala</surname><given-names>Susanne</given-names></name><xref ref-type="aff" rid="A1">a</xref><xref ref-type="aff" rid="A3">c</xref></contrib></contrib-group><aff id="A1"><label>a</label>Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria</aff><aff id="A2"><label>b</label>Christian Doppler Laboratories for Allergy Research, Medical University of Vienna, Vienna, Austria</aff><aff id="A3"><label>c</label>Development of Allergen Chips, Medical University of Vienna, Vienna, Austria</aff><aff id="A4"><label>d</label>Center for Molecular Allergology, IDI-IRCCS, Rome, Italy</aff><aff id="A5"><label>e</label>Division of Asthma and Allergology, Department of Chest Diseases, Strasbourg University Hospital, Strasbourg, France</aff><aff id="A6"><label>f</label>Center of Child Health Research, University of Western Australia, Telethon Institute for Child Health Research, Perth, W.A., Australia</aff><author-notes><corresp id="CR1">Correspondence to: Dr. Susanne Vrtala, Division of Immunopathology, Department of Pathophysiology and Allergy Research Center for Pathophysiology, Infectiology and Immunology, Vienna General Hospital Medical University of Vienna, Waehringer Guertel 18–20, AT–1090 Vienna (Austria), Tel. +43 140 400 5132, <email>susanne.vrtala@meduniwien.ac.at</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>17</day><month>9</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>21</day><month>6</month><year>2012</year></pub-date><pub-date pub-type="ppub"><year>2012</year></pub-date><pub-date pub-type="pmc-release"><day>06</day><month>10</month><year>2015</year></pub-date><volume>159</volume><issue>3</issue><fpage>253</fpage><lpage>262</lpage><pmc-comment>elocation-id from pubmed: 10.1159/000337654</pmc-comment>
<abstract><sec id="S1"><title>Background</title><p id="P1">Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available <italic>Dermatophagoides pteronyssinus</italic> extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity.</p></sec><sec id="S2"><title>Methods</title><p id="P2">Antibodies specific for several <italic>D. pteronyssinus</italic> allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot.</p></sec><sec id="S3"><title>Results</title><p id="P3">Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0–40.8 μg ml<sup>−1</sup>; Der p 2: 1.7–45.0 μg ml<sup>−1</sup>). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">Commercially available <italic>D. pteronyssinus</italic> extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.</p></sec></abstract><kwd-group><kwd>House-dust mites</kwd><kwd>Allergen extracts</kwd><kwd><italic>Dermatophagoides pteronyssinus</italic></kwd><kwd>Skin prick tests</kwd><kwd>Recombinant allergens</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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