Regulation of Meristem Morphogenesis by Cell Wall Synthases in Arabidopsis
Identifieur interne : 002076 ( Pmc/Corpus ); précédent : 002075; suivant : 002077Regulation of Meristem Morphogenesis by Cell Wall Synthases in Arabidopsis
Auteurs : Weibing Yang ; Christoph Schuster ; Cherie T. Beahan ; Varodom Charoensawan ; Alexis Peaucelle ; Antony Bacic ; Monika S. Doblin ; Raymond Wightman ; Elliot M. MeyerowitzSource :
- Current biology : CB [ 0960-9822 ] ; 2016.
Abstract
The cell walls of the shoot apical meristem (SAM), containing the stem cell niche that gives rise to the above-ground tissues, are crucially involved in regulating differentiation. It is currently unknown how these walls are built and refined or their role, if any, in influencing meristem developmental dynamics. We have combined polysaccharide linkage analysis, immuno-labelling and transcriptome profiling of the SAM to provide a spatio-temporal plan of the walls of this dynamic structure. We find that meristematic cells express only a core subset of 152 genes encoding cell wall glycosyltransferases (GTs). Systemic localization of all these GT mRNAs by
Url:
DOI: 10.1016/j.cub.2016.04.026
PubMed: 27212401
PubMed Central: 5024349
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PMC:5024349Le document en format XML
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<author><name sortKey="Yang, Weibing" sort="Yang, Weibing" uniqKey="Yang W" first="Weibing" last="Yang">Weibing Yang</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
</affiliation>
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<author><name sortKey="Schuster, Christoph" sort="Schuster, Christoph" uniqKey="Schuster C" first="Christoph" last="Schuster">Christoph Schuster</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
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<author><name sortKey="Beahan, Cherie T" sort="Beahan, Cherie T" uniqKey="Beahan C" first="Cherie T." last="Beahan">Cherie T. Beahan</name>
<affiliation><nlm:aff id="A2">ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Parkville, Victoria 3010. Australia</nlm:aff>
</affiliation>
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<author><name sortKey="Charoensawan, Varodom" sort="Charoensawan, Varodom" uniqKey="Charoensawan V" first="Varodom" last="Charoensawan">Varodom Charoensawan</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A4">Integrative Computational BioScience (ICBS) Center, Mahidol University, Nakhon Pathom 73170, Thailandz</nlm:aff>
</affiliation>
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<author><name sortKey="Peaucelle, Alexis" sort="Peaucelle, Alexis" uniqKey="Peaucelle A" first="Alexis" last="Peaucelle">Alexis Peaucelle</name>
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<affiliation><nlm:aff id="A5">Institut Jean-Pierre Bourgin, UMR1318, Institut National pour la Recherche Agronomique-AgroParisTech, Saclay Plant Science, Route De St-Cyr, Versailles 78026, France</nlm:aff>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Regulation of Meristem Morphogenesis by Cell Wall Synthases in <italic>Arabidopsis</italic>
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<author><name sortKey="Yang, Weibing" sort="Yang, Weibing" uniqKey="Yang W" first="Weibing" last="Yang">Weibing Yang</name>
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<author><name sortKey="Schuster, Christoph" sort="Schuster, Christoph" uniqKey="Schuster C" first="Christoph" last="Schuster">Christoph Schuster</name>
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</affiliation>
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<author><name sortKey="Beahan, Cherie T" sort="Beahan, Cherie T" uniqKey="Beahan C" first="Cherie T." last="Beahan">Cherie T. Beahan</name>
<affiliation><nlm:aff id="A2">ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Parkville, Victoria 3010. Australia</nlm:aff>
</affiliation>
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<author><name sortKey="Charoensawan, Varodom" sort="Charoensawan, Varodom" uniqKey="Charoensawan V" first="Varodom" last="Charoensawan">Varodom Charoensawan</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A4">Integrative Computational BioScience (ICBS) Center, Mahidol University, Nakhon Pathom 73170, Thailandz</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Peaucelle, Alexis" sort="Peaucelle, Alexis" uniqKey="Peaucelle A" first="Alexis" last="Peaucelle">Alexis Peaucelle</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A5">Institut Jean-Pierre Bourgin, UMR1318, Institut National pour la Recherche Agronomique-AgroParisTech, Saclay Plant Science, Route De St-Cyr, Versailles 78026, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bacic, Antony" sort="Bacic, Antony" uniqKey="Bacic A" first="Antony" last="Bacic">Antony Bacic</name>
<affiliation><nlm:aff id="A2">ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Parkville, Victoria 3010. Australia</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Doblin, Monika S" sort="Doblin, Monika S" uniqKey="Doblin M" first="Monika S" last="Doblin">Monika S. Doblin</name>
<affiliation><nlm:aff id="A2">ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Parkville, Victoria 3010. Australia</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wightman, Raymond" sort="Wightman, Raymond" uniqKey="Wightman R" first="Raymond" last="Wightman">Raymond Wightman</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Meyerowitz, Elliot M" sort="Meyerowitz, Elliot M" uniqKey="Meyerowitz E" first="Elliot M." last="Meyerowitz">Elliot M. Meyerowitz</name>
<affiliation><nlm:aff id="A1">Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A6">Howard Hughes Medical Institute and Division of Biology and Biological Engineering, 156-29 California Institute of Technology, Pasadena. CA 91125. U.S.A</nlm:aff>
</affiliation>
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<idno type="ISSN">0960-9822</idno>
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<front><div type="abstract" xml:lang="en"><title>SUMMARY</title>
<p id="P2">The cell walls of the shoot apical meristem (SAM), containing the stem cell niche that gives rise to the above-ground tissues, are crucially involved in regulating differentiation. It is currently unknown how these walls are built and refined or their role, if any, in influencing meristem developmental dynamics. We have combined polysaccharide linkage analysis, immuno-labelling and transcriptome profiling of the SAM to provide a spatio-temporal plan of the walls of this dynamic structure. We find that meristematic cells express only a core subset of 152 genes encoding cell wall glycosyltransferases (GTs). Systemic localization of all these GT mRNAs by <italic>in situ</italic>
hybridization reveals members with either enrichment in or specificity to apical subdomains such as emerging flower primordia, and a large class with high expression in dividing cells. The highly localized and coordinated expression of GTs in the SAM suggests distinct wall properties of meristematic cells, and specific differences between newly forming walls and their mature descendants. Functional analysis demonstrates that a subset of <italic>CSLD</italic>
genes is essential for proper meristem maintenance, confirming the key role of walls in developmental pathways.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9107782</journal-id>
<journal-id journal-id-type="pubmed-jr-id">8548</journal-id>
<journal-id journal-id-type="nlm-ta">Curr Biol</journal-id>
<journal-id journal-id-type="iso-abbrev">Curr. Biol.</journal-id>
<journal-title-group><journal-title>Current biology : CB</journal-title>
</journal-title-group>
<issn pub-type="ppub">0960-9822</issn>
<issn pub-type="epub">1879-0445</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27212401</article-id>
<article-id pub-id-type="pmc">5024349</article-id>
<article-id pub-id-type="doi">10.1016/j.cub.2016.04.026</article-id>
<article-id pub-id-type="manuscript">HHMIMS812306</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Regulation of Meristem Morphogenesis by Cell Wall Synthases in <italic>Arabidopsis</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Weibing</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Schuster</surname>
<given-names>Christoph</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Beahan</surname>
<given-names>Cherie T.</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Charoensawan</surname>
<given-names>Varodom</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A3">3</xref>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Peaucelle</surname>
<given-names>Alexis</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A5">5</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bacic</surname>
<given-names>Antony</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Doblin</surname>
<given-names>Monika S</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wightman</surname>
<given-names>Raymond</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Meyerowitz</surname>
<given-names>Elliot M.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A6">6</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR. U.K</aff>
<aff id="A2"><label>2</label>
ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Parkville, Victoria 3010. Australia</aff>
<aff id="A3"><label>3</label>
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand</aff>
<aff id="A4"><label>4</label>
Integrative Computational BioScience (ICBS) Center, Mahidol University, Nakhon Pathom 73170, Thailandz</aff>
<aff id="A5"><label>5</label>
Institut Jean-Pierre Bourgin, UMR1318, Institut National pour la Recherche Agronomique-AgroParisTech, Saclay Plant Science, Route De St-Cyr, Versailles 78026, France</aff>
<aff id="A6"><label>6</label>
Howard Hughes Medical Institute and Division of Biology and Biological Engineering, 156-29 California Institute of Technology, Pasadena. CA 91125. U.S.A</aff>
<author-notes><corresp id="FN1"><label>*</label>
Correspondence to: <email>meyerow@caltech.edu</email>
, <email>msdoblin@unimelb.edu.au</email>
, <email>raymond.wightman@slcu.cam.ac.uk</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>24</day>
<month>8</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub"><day>19</day>
<month>5</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="ppub"><day>6</day>
<month>6</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>06</day>
<month>6</month>
<year>2017</year>
</pub-date>
<volume>26</volume>
<issue>11</issue>
<fpage>1404</fpage>
<lpage>1415</lpage>
<pmc-comment>elocation-id from pubmed: 10.1016/j.cub.2016.04.026</pmc-comment>
<abstract><title>SUMMARY</title>
<p id="P2">The cell walls of the shoot apical meristem (SAM), containing the stem cell niche that gives rise to the above-ground tissues, are crucially involved in regulating differentiation. It is currently unknown how these walls are built and refined or their role, if any, in influencing meristem developmental dynamics. We have combined polysaccharide linkage analysis, immuno-labelling and transcriptome profiling of the SAM to provide a spatio-temporal plan of the walls of this dynamic structure. We find that meristematic cells express only a core subset of 152 genes encoding cell wall glycosyltransferases (GTs). Systemic localization of all these GT mRNAs by <italic>in situ</italic>
hybridization reveals members with either enrichment in or specificity to apical subdomains such as emerging flower primordia, and a large class with high expression in dividing cells. The highly localized and coordinated expression of GTs in the SAM suggests distinct wall properties of meristematic cells, and specific differences between newly forming walls and their mature descendants. Functional analysis demonstrates that a subset of <italic>CSLD</italic>
genes is essential for proper meristem maintenance, confirming the key role of walls in developmental pathways.</p>
</abstract>
</article-meta>
</front>
</pmc>
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