Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Using Fourier transform IR spectroscopy to analyze biological materials</title><author><name sortKey="Baker, Matthew J" sort="Baker, Matthew J" uniqKey="Baker M" first="Matthew J" last="Baker">Matthew J. Baker</name><affiliation><nlm:aff id="A1">Centre for Materials Science, Division of Chemistry, University of Central Lancashire, Preston, UK</nlm:aff></affiliation></author><author><name sortKey="Trevisan, Julio" sort="Trevisan, Julio" uniqKey="Trevisan J" first="Júlio" last="Trevisan">Júlio Trevisan</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation><affiliation><nlm:aff id="A3">School of Computing and Communications, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Bassan, Paul" sort="Bassan, Paul" uniqKey="Bassan P" first="Paul" last="Bassan">Paul Bassan</name><affiliation><nlm:aff id="A4">Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</nlm:aff></affiliation></author><author><name sortKey="Bhargava, Rohit" sort="Bhargava, Rohit" uniqKey="Bhargava R" first="Rohit" last="Bhargava">Rohit Bhargava</name><affiliation><nlm:aff id="A5">Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Butler, Holly J" sort="Butler, Holly J" uniqKey="Butler H" first="Holly J" last="Butler">Holly J. Butler</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Dorling, Konrad M" sort="Dorling, Konrad M" uniqKey="Dorling K" first="Konrad M" last="Dorling">Konrad M. Dorling</name><affiliation><nlm:aff id="A1">Centre for Materials Science, Division of Chemistry, University of Central Lancashire, Preston, UK</nlm:aff></affiliation></author><author><name sortKey="Fielden, Peter R" sort="Fielden, Peter R" uniqKey="Fielden P" first="Peter R" last="Fielden">Peter R. Fielden</name><affiliation><nlm:aff id="A6">Department of Chemistry, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Fogarty, Simon W" sort="Fogarty, Simon W" uniqKey="Fogarty S" first="Simon W" last="Fogarty">Simon W. Fogarty</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation><affiliation><nlm:aff id="A7">Division of Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Fullwood, Nigel J" sort="Fullwood, Nigel J" uniqKey="Fullwood N" first="Nigel J" last="Fullwood">Nigel J. Fullwood</name><affiliation><nlm:aff id="A7">Division of Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Heys, Kelly A" sort="Heys, Kelly A" uniqKey="Heys K" first="Kelly A" last="Heys">Kelly A. Heys</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Hughes, Caryn" sort="Hughes, Caryn" uniqKey="Hughes C" first="Caryn" last="Hughes">Caryn Hughes</name><affiliation><nlm:aff id="A4">Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</nlm:aff></affiliation></author><author><name sortKey="Lasch, Peter" sort="Lasch, Peter" uniqKey="Lasch P" first="Peter" last="Lasch">Peter Lasch</name><affiliation><nlm:aff id="A8">Proteomics and Spectroscopy (ZBS 6), Robert-Koch-Institut, Berlin, Germany</nlm:aff></affiliation></author><author><name sortKey="Martin Hirsch, Pierre L" sort="Martin Hirsch, Pierre L" uniqKey="Martin Hirsch P" first="Pierre L" last="Martin-Hirsch">Pierre L. Martin-Hirsch</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Obinaju, Blessing" sort="Obinaju, Blessing" uniqKey="Obinaju B" first="Blessing" last="Obinaju">Blessing Obinaju</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Sockalingum, Ganesh D" sort="Sockalingum, Ganesh D" uniqKey="Sockalingum G" first="Ganesh D" last="Sockalingum">Ganesh D. Sockalingum</name><affiliation><nlm:aff id="A9">Equipe MéDIAN-Biophotonique et Technologies pour la Santé, Université de Reims Champagne-Ardenne, UnitéMEDyC, CNRS UMR7369, UFR Pharmacie, SFR CAP-Santé FED4231, Reims, France</nlm:aff></affiliation></author><author><name sortKey="Sule Suso, Josep" sort="Sule Suso, Josep" uniqKey="Sule Suso J" first="Josep" last="Sulé-Suso">Josep Sulé-Suso</name><affiliation><nlm:aff id="A10">Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, UK</nlm:aff></affiliation></author><author><name sortKey="Strong, Rebecca J" sort="Strong, Rebecca J" uniqKey="Strong R" first="Rebecca J" last="Strong">Rebecca J. Strong</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Walsh, Michael J" sort="Walsh, Michael J" uniqKey="Walsh M" first="Michael J" last="Walsh">Michael J. Walsh</name><affiliation><nlm:aff id="A11">Department of Pathology, College of Medicine Research Building (COMRB), University of Illinois at Chicago, Chicago, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Wood, Bayden R" sort="Wood, Bayden R" uniqKey="Wood B" first="Bayden R" last="Wood">Bayden R. Wood</name><affiliation><nlm:aff id="A12">Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation></author><author><name sortKey="Gardner, Peter" sort="Gardner, Peter" uniqKey="Gardner P" first="Peter" last="Gardner">Peter Gardner</name><affiliation><nlm:aff id="A4">Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</nlm:aff></affiliation></author><author><name sortKey="Martin, Francis L" sort="Martin, Francis L" uniqKey="Martin F" first="Francis L" last="Martin">Francis L. Martin</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24992094</idno><idno type="pmc">4480339</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4480339</idno><idno type="RBID">PMC:4480339</idno><idno type="doi">10.1038/nprot.2014.110</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">001E43</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001E43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Using Fourier transform IR spectroscopy to analyze biological materials</title><author><name sortKey="Baker, Matthew J" sort="Baker, Matthew J" uniqKey="Baker M" first="Matthew J" last="Baker">Matthew J. Baker</name><affiliation><nlm:aff id="A1">Centre for Materials Science, Division of Chemistry, University of Central Lancashire, Preston, UK</nlm:aff></affiliation></author><author><name sortKey="Trevisan, Julio" sort="Trevisan, Julio" uniqKey="Trevisan J" first="Júlio" last="Trevisan">Júlio Trevisan</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation><affiliation><nlm:aff id="A3">School of Computing and Communications, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Bassan, Paul" sort="Bassan, Paul" uniqKey="Bassan P" first="Paul" last="Bassan">Paul Bassan</name><affiliation><nlm:aff id="A4">Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</nlm:aff></affiliation></author><author><name sortKey="Bhargava, Rohit" sort="Bhargava, Rohit" uniqKey="Bhargava R" first="Rohit" last="Bhargava">Rohit Bhargava</name><affiliation><nlm:aff id="A5">Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Butler, Holly J" sort="Butler, Holly J" uniqKey="Butler H" first="Holly J" last="Butler">Holly J. Butler</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Dorling, Konrad M" sort="Dorling, Konrad M" uniqKey="Dorling K" first="Konrad M" last="Dorling">Konrad M. Dorling</name><affiliation><nlm:aff id="A1">Centre for Materials Science, Division of Chemistry, University of Central Lancashire, Preston, UK</nlm:aff></affiliation></author><author><name sortKey="Fielden, Peter R" sort="Fielden, Peter R" uniqKey="Fielden P" first="Peter R" last="Fielden">Peter R. Fielden</name><affiliation><nlm:aff id="A6">Department of Chemistry, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Fogarty, Simon W" sort="Fogarty, Simon W" uniqKey="Fogarty S" first="Simon W" last="Fogarty">Simon W. Fogarty</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation><affiliation><nlm:aff id="A7">Division of Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Fullwood, Nigel J" sort="Fullwood, Nigel J" uniqKey="Fullwood N" first="Nigel J" last="Fullwood">Nigel J. Fullwood</name><affiliation><nlm:aff id="A7">Division of Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Heys, Kelly A" sort="Heys, Kelly A" uniqKey="Heys K" first="Kelly A" last="Heys">Kelly A. Heys</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Hughes, Caryn" sort="Hughes, Caryn" uniqKey="Hughes C" first="Caryn" last="Hughes">Caryn Hughes</name><affiliation><nlm:aff id="A4">Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</nlm:aff></affiliation></author><author><name sortKey="Lasch, Peter" sort="Lasch, Peter" uniqKey="Lasch P" first="Peter" last="Lasch">Peter Lasch</name><affiliation><nlm:aff id="A8">Proteomics and Spectroscopy (ZBS 6), Robert-Koch-Institut, Berlin, Germany</nlm:aff></affiliation></author><author><name sortKey="Martin Hirsch, Pierre L" sort="Martin Hirsch, Pierre L" uniqKey="Martin Hirsch P" first="Pierre L" last="Martin-Hirsch">Pierre L. Martin-Hirsch</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Obinaju, Blessing" sort="Obinaju, Blessing" uniqKey="Obinaju B" first="Blessing" last="Obinaju">Blessing Obinaju</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Sockalingum, Ganesh D" sort="Sockalingum, Ganesh D" uniqKey="Sockalingum G" first="Ganesh D" last="Sockalingum">Ganesh D. Sockalingum</name><affiliation><nlm:aff id="A9">Equipe MéDIAN-Biophotonique et Technologies pour la Santé, Université de Reims Champagne-Ardenne, UnitéMEDyC, CNRS UMR7369, UFR Pharmacie, SFR CAP-Santé FED4231, Reims, France</nlm:aff></affiliation></author><author><name sortKey="Sule Suso, Josep" sort="Sule Suso, Josep" uniqKey="Sule Suso J" first="Josep" last="Sulé-Suso">Josep Sulé-Suso</name><affiliation><nlm:aff id="A10">Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, UK</nlm:aff></affiliation></author><author><name sortKey="Strong, Rebecca J" sort="Strong, Rebecca J" uniqKey="Strong R" first="Rebecca J" last="Strong">Rebecca J. Strong</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author><author><name sortKey="Walsh, Michael J" sort="Walsh, Michael J" uniqKey="Walsh M" first="Michael J" last="Walsh">Michael J. Walsh</name><affiliation><nlm:aff id="A11">Department of Pathology, College of Medicine Research Building (COMRB), University of Illinois at Chicago, Chicago, Illinois, USA</nlm:aff></affiliation></author><author><name sortKey="Wood, Bayden R" sort="Wood, Bayden R" uniqKey="Wood B" first="Bayden R" last="Wood">Bayden R. Wood</name><affiliation><nlm:aff id="A12">Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation></author><author><name sortKey="Gardner, Peter" sort="Gardner, Peter" uniqKey="Gardner P" first="Peter" last="Gardner">Peter Gardner</name><affiliation><nlm:aff id="A4">Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</nlm:aff></affiliation></author><author><name sortKey="Martin, Francis L" sort="Martin, Francis L" uniqKey="Martin F" first="Francis L" last="Martin">Francis L. Martin</name><affiliation><nlm:aff id="A2">Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</nlm:aff></affiliation></author></analytic><series><title level="j">Nature protocols</title><idno type="ISSN">1754-2189</idno><idno type="eISSN">1750-2799</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">IR spectroscopy is an excellent method for biological analyses. It enables the nonperturbative, label-free extraction of biochemical information and images toward diagnosis and the assessment of cell functionality. Although not strictly microscopy in the conventional sense, it allows the construction of images of tissue or cell architecture by the passing of spectral data through a variety of computational algorithms. Because such images are constructed from fingerprint spectra, the notion is that they can be an objective reflection of the underlying health status of the analyzed sample. One of the major difficulties in the field has been determining a consensus on spectral pre-processing and data analysis. This manuscript brings together as coauthors some of the leaders in this field to allow the standardization of methods and procedures for adapting a multistage approach to a methodology that can be applied to a variety of cell biological questions or used within a clinical setting for disease screening or diagnosis. We describe a protocol for collecting IR spectra and images from biological samples (e.g., fixed cytology and tissue sections, live cells or biofluids) that assesses the instrumental options available, appropriate sample preparation, different sampling modes as well as important advances in spectral data acquisition. After acquisition, data processing consists of a sequence of steps including quality control, spectral pre-processing, feature extraction and classification of the supervised or unsupervised type. A typical experiment can be completed and analyzed within hours. Example results are presented on the use of IR spectra combined with multivariate data processing.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101284307</journal-id><journal-id journal-id-type="pubmed-jr-id">33047</journal-id><journal-id journal-id-type="nlm-ta">Nat Protoc</journal-id><journal-id journal-id-type="iso-abbrev">Nat Protoc</journal-id><journal-title-group><journal-title>Nature protocols</journal-title></journal-title-group><issn pub-type="ppub">1754-2189</issn><issn pub-type="epub">1750-2799</issn></journal-meta><article-meta><article-id pub-id-type="pmid">24992094</article-id><article-id pub-id-type="pmc">4480339</article-id><article-id pub-id-type="doi">10.1038/nprot.2014.110</article-id><article-id pub-id-type="manuscript">NIHMS700198</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Using Fourier transform IR spectroscopy to analyze biological materials</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Baker</surname><given-names>Matthew J</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="author-notes" rid="FN1">13</xref></contrib><contrib contrib-type="author"><name><surname>Trevisan</surname><given-names>Júlio</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Bassan</surname><given-names>Paul</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Bhargava</surname><given-names>Rohit</given-names></name><xref ref-type="aff" rid="A5">5</xref></contrib><contrib contrib-type="author"><name><surname>Butler</surname><given-names>Holly J</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Dorling</surname><given-names>Konrad M</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Fielden</surname><given-names>Peter R</given-names></name><xref ref-type="aff" rid="A6">6</xref></contrib><contrib contrib-type="author"><name><surname>Fogarty</surname><given-names>Simon W</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A7">7</xref></contrib><contrib contrib-type="author"><name><surname>Fullwood</surname><given-names>Nigel J</given-names></name><xref ref-type="aff" rid="A7">7</xref></contrib><contrib contrib-type="author"><name><surname>Heys</surname><given-names>Kelly A</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Hughes</surname><given-names>Caryn</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Lasch</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="A8">8</xref></contrib><contrib contrib-type="author"><name><surname>Martin-Hirsch</surname><given-names>Pierre L</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Obinaju</surname><given-names>Blessing</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Sockalingum</surname><given-names>Ganesh D</given-names></name><xref ref-type="aff" rid="A9">9</xref></contrib><contrib contrib-type="author"><name><surname>Sulé-Suso</surname><given-names>Josep</given-names></name><xref ref-type="aff" rid="A10">10</xref></contrib><contrib contrib-type="author"><name><surname>Strong</surname><given-names>Rebecca J</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Walsh</surname><given-names>Michael J</given-names></name><xref ref-type="aff" rid="A11">11</xref></contrib><contrib contrib-type="author"><name><surname>Wood</surname><given-names>Bayden R</given-names></name><xref ref-type="aff" rid="A12">12</xref></contrib><contrib contrib-type="author"><name><surname>Gardner</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Martin</surname><given-names>Francis L</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib></contrib-group><aff id="A1"><label>1</label>Centre for Materials Science, Division of Chemistry, University of Central Lancashire, Preston, UK</aff><aff id="A2"><label>2</label>Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, UK</aff><aff id="A3"><label>3</label>School of Computing and Communications, Lancaster University, Lancaster, UK</aff><aff id="A4"><label>4</label>Manchester Institute of Biotechnology (MIB), University of Manchester, Manchester, UK</aff><aff id="A5"><label>5</label>Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA</aff><aff id="A6"><label>6</label>Department of Chemistry, Lancaster University, Lancaster, UK</aff><aff id="A7"><label>7</label>Division of Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK</aff><aff id="A8"><label>8</label>Proteomics and Spectroscopy (ZBS 6), Robert-Koch-Institut, Berlin, Germany</aff><aff id="A9"><label>9</label>Equipe MéDIAN-Biophotonique et Technologies pour la Santé, Université de Reims Champagne-Ardenne, UnitéMEDyC, CNRS UMR7369, UFR Pharmacie, SFR CAP-Santé FED4231, Reims, France</aff><aff id="A10"><label>10</label>Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, UK</aff><aff id="A11"><label>11</label>Department of Pathology, College of Medicine Research Building (COMRB), University of Illinois at Chicago, Chicago, Illinois, USA</aff><aff id="A12"><label>12</label>Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Victoria, Australia</aff><author-notes><corresp id="CR1">Correspondence should be addressed to F.L.M. (<email>f.martin@lancaster.ac.uk</email>).</corresp><fn fn-type="present-address" id="FN1"><label>13</label><p id="P1">Present address: WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.</p></fn></author-notes><pub-date pub-type="nihms-submitted"><day>16</day><month>6</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>03</day><month>7</month><year>2014</year></pub-date><pub-date pub-type="ppub"><month>8</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>8</month><year>2015</year></pub-date><volume>9</volume><issue>8</issue><fpage>1771</fpage><lpage>1791</lpage><pmc-comment>elocation-id from pubmed: 10.1038/nprot.2014.110</pmc-comment>
<permissions><copyright-statement>© 2014 Nature America, Inc. All rights reserved.</copyright-statement><copyright-year>2014</copyright-year><license license-type="permissions-link"><license-p>Reprints and permissions information is available online at <ext-link ext-link-type="uri" xlink:href="http://www.nature.com/reprints/index.html">http://www.nature.com/reprints/index.html</ext-link>.</license-p></license></permissions><abstract><p id="P2">IR spectroscopy is an excellent method for biological analyses. It enables the nonperturbative, label-free extraction of biochemical information and images toward diagnosis and the assessment of cell functionality. Although not strictly microscopy in the conventional sense, it allows the construction of images of tissue or cell architecture by the passing of spectral data through a variety of computational algorithms. Because such images are constructed from fingerprint spectra, the notion is that they can be an objective reflection of the underlying health status of the analyzed sample. One of the major difficulties in the field has been determining a consensus on spectral pre-processing and data analysis. This manuscript brings together as coauthors some of the leaders in this field to allow the standardization of methods and procedures for adapting a multistage approach to a methodology that can be applied to a variety of cell biological questions or used within a clinical setting for disease screening or diagnosis. We describe a protocol for collecting IR spectra and images from biological samples (e.g., fixed cytology and tissue sections, live cells or biofluids) that assesses the instrumental options available, appropriate sample preparation, different sampling modes as well as important advances in spectral data acquisition. After acquisition, data processing consists of a sequence of steps including quality control, spectral pre-processing, feature extraction and classification of the supervised or unsupervised type. A typical experiment can be completed and analyzed within hours. Example results are presented on the use of IR spectra combined with multivariate data processing.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E43 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001E43 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.33. |  |