Mitochondrial DNA from the eradicated European Plasmodium vivax and P. falciparum from 70-year-old slides from the Ebro Delta in Spain
Identifieur interne : 001C06 ( Pmc/Corpus ); précédent : 001C05; suivant : 001C07Mitochondrial DNA from the eradicated European Plasmodium vivax and P. falciparum from 70-year-old slides from the Ebro Delta in Spain
Auteurs : Pere Gelabert ; Marcela Sandoval-Velasco ; I Igo Olalde ; Rosa Fregel ; Adrien Rieux ; Raül Escosa ; Carles Aranda ; Krijn Paaijmans ; Ivo Mueller ; M. Thomas P. Gilbert ; Carles Lalueza-FoxSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2016.
Abstract
Previous studies have attempted to unravel the complex phylogeographic patterns of the malaria parasites
Url:
DOI: 10.1073/pnas.1611017113
PubMed: 27671660
PubMed Central: 5068322
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mitochondrial DNA from the eradicated European <italic>Plasmodium vivax</italic> and <italic>P. falciparum</italic> from 70-year-old slides from the Ebro Delta in Spain</title><author><name sortKey="Gelabert, Pere" sort="Gelabert, Pere" uniqKey="Gelabert P" first="Pere" last="Gelabert">Pere Gelabert</name><affiliation><nlm:aff id="aff1"><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Sandoval Velasco, Marcela" sort="Sandoval Velasco, Marcela" uniqKey="Sandoval Velasco M" first="Marcela" last="Sandoval-Velasco">Marcela Sandoval-Velasco</name><affiliation><nlm:aff id="aff2">EvoGenomics, Natural History Museum of Denmark,<institution>University of Copenhagen</institution>, 1350 Copenhagen,<country>Denmark</country>;</nlm:aff></affiliation></author><author><name sortKey="Olalde, I Igo" sort="Olalde, I Igo" uniqKey="Olalde I" first="I Igo" last="Olalde">I Igo Olalde</name><affiliation><nlm:aff id="aff1"><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Fregel, Rosa" sort="Fregel, Rosa" uniqKey="Fregel R" first="Rosa" last="Fregel">Rosa Fregel</name><affiliation><nlm:aff id="aff3">Department of Genetics,<institution>Stanford University</institution>, Stanford,<addr-line>CA</addr-line> 94305;</nlm:aff></affiliation></author><author><name sortKey="Rieux, Adrien" sort="Rieux, Adrien" uniqKey="Rieux A" first="Adrien" last="Rieux">Adrien Rieux</name><affiliation><nlm:aff id="aff4">Agricultural Research for Development (CIRAD), Systèmes biologiques (BIOS) Department,<institution>Peuplements végétaux et bioagresseurs en milieu tropical (PVBMT) Laboratory</institution>, 97410 St. Pierre de la Réunion,<country>France</country>;</nlm:aff></affiliation></author><author><name sortKey="Escosa, Raul" sort="Escosa, Raul" uniqKey="Escosa R" first="Raül" last="Escosa">Raül Escosa</name><affiliation><nlm:aff id="aff5"><institution>Consorci de Polítiques Ambientals de les Terres de l’Ebre</institution>, 43580 Deltebre,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Aranda, Carles" sort="Aranda, Carles" uniqKey="Aranda C" first="Carles" last="Aranda">Carles Aranda</name><affiliation><nlm:aff id="aff6">Servei de Control de Mosquits,<institution>Consell Comarcal del Baix Llobregat</institution>, 08980 Sant Feliu de Llobregat,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Paaijmans, Krijn" sort="Paaijmans, Krijn" uniqKey="Paaijmans K" first="Krijn" last="Paaijmans">Krijn Paaijmans</name><affiliation><nlm:aff id="aff7">ISGlobal, Barcelona Institute for Global Health,<institution>Hospital Clínic-Universitat de Barcelona</institution>, 08036 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name><affiliation><nlm:aff id="aff7">ISGlobal, Barcelona Institute for Global Health,<institution>Hospital Clínic-Universitat de Barcelona</institution>, 08036 Barcelona,<country>Spain</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">Population Health and Immunity Division,<institution>Walter & Eliza Hall Institute</institution>, Parkville, 3052 VIC,<country>Australia</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Department of Medical Biology,<institution>University of Melbourne</institution>, Parkville, 3052 VIC,<country>Australia</country>;</nlm:aff></affiliation></author><author><name sortKey="Gilbert, M Thomas P" sort="Gilbert, M Thomas P" uniqKey="Gilbert M" first="M. Thomas P." last="Gilbert">M. Thomas P. Gilbert</name><affiliation><nlm:aff id="aff2">EvoGenomics, Natural History Museum of Denmark,<institution>University of Copenhagen</institution>, 1350 Copenhagen,<country>Denmark</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff10">Trace and Environmental DNA Laboratory, Department of Environment and Agriculture,<institution>Curtin University</institution>, Perth, 6102 WA,<country>Australia</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff11"><institution>Norwegian University of Science and Technology (NTNU) University Museum</institution>, N-7491 Trondheim,<country>Norway</country></nlm:aff></affiliation></author><author><name sortKey="Lalueza Fox, Carles" sort="Lalueza Fox, Carles" uniqKey="Lalueza Fox C" first="Carles" last="Lalueza-Fox">Carles Lalueza-Fox</name><affiliation><nlm:aff id="aff1"><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27671660</idno><idno type="pmc">5068322</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068322</idno><idno type="RBID">PMC:5068322</idno><idno type="doi">10.1073/pnas.1611017113</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">001C06</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001C06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Mitochondrial DNA from the eradicated European <italic>Plasmodium vivax</italic> and <italic>P. falciparum</italic> from 70-year-old slides from the Ebro Delta in Spain</title><author><name sortKey="Gelabert, Pere" sort="Gelabert, Pere" uniqKey="Gelabert P" first="Pere" last="Gelabert">Pere Gelabert</name><affiliation><nlm:aff id="aff1"><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Sandoval Velasco, Marcela" sort="Sandoval Velasco, Marcela" uniqKey="Sandoval Velasco M" first="Marcela" last="Sandoval-Velasco">Marcela Sandoval-Velasco</name><affiliation><nlm:aff id="aff2">EvoGenomics, Natural History Museum of Denmark,<institution>University of Copenhagen</institution>, 1350 Copenhagen,<country>Denmark</country>;</nlm:aff></affiliation></author><author><name sortKey="Olalde, I Igo" sort="Olalde, I Igo" uniqKey="Olalde I" first="I Igo" last="Olalde">I Igo Olalde</name><affiliation><nlm:aff id="aff1"><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Fregel, Rosa" sort="Fregel, Rosa" uniqKey="Fregel R" first="Rosa" last="Fregel">Rosa Fregel</name><affiliation><nlm:aff id="aff3">Department of Genetics,<institution>Stanford University</institution>, Stanford,<addr-line>CA</addr-line> 94305;</nlm:aff></affiliation></author><author><name sortKey="Rieux, Adrien" sort="Rieux, Adrien" uniqKey="Rieux A" first="Adrien" last="Rieux">Adrien Rieux</name><affiliation><nlm:aff id="aff4">Agricultural Research for Development (CIRAD), Systèmes biologiques (BIOS) Department,<institution>Peuplements végétaux et bioagresseurs en milieu tropical (PVBMT) Laboratory</institution>, 97410 St. Pierre de la Réunion,<country>France</country>;</nlm:aff></affiliation></author><author><name sortKey="Escosa, Raul" sort="Escosa, Raul" uniqKey="Escosa R" first="Raül" last="Escosa">Raül Escosa</name><affiliation><nlm:aff id="aff5"><institution>Consorci de Polítiques Ambientals de les Terres de l’Ebre</institution>, 43580 Deltebre,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Aranda, Carles" sort="Aranda, Carles" uniqKey="Aranda C" first="Carles" last="Aranda">Carles Aranda</name><affiliation><nlm:aff id="aff6">Servei de Control de Mosquits,<institution>Consell Comarcal del Baix Llobregat</institution>, 08980 Sant Feliu de Llobregat,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Paaijmans, Krijn" sort="Paaijmans, Krijn" uniqKey="Paaijmans K" first="Krijn" last="Paaijmans">Krijn Paaijmans</name><affiliation><nlm:aff id="aff7">ISGlobal, Barcelona Institute for Global Health,<institution>Hospital Clínic-Universitat de Barcelona</institution>, 08036 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author><author><name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name><affiliation><nlm:aff id="aff7">ISGlobal, Barcelona Institute for Global Health,<institution>Hospital Clínic-Universitat de Barcelona</institution>, 08036 Barcelona,<country>Spain</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">Population Health and Immunity Division,<institution>Walter & Eliza Hall Institute</institution>, Parkville, 3052 VIC,<country>Australia</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Department of Medical Biology,<institution>University of Melbourne</institution>, Parkville, 3052 VIC,<country>Australia</country>;</nlm:aff></affiliation></author><author><name sortKey="Gilbert, M Thomas P" sort="Gilbert, M Thomas P" uniqKey="Gilbert M" first="M. Thomas P." last="Gilbert">M. Thomas P. Gilbert</name><affiliation><nlm:aff id="aff2">EvoGenomics, Natural History Museum of Denmark,<institution>University of Copenhagen</institution>, 1350 Copenhagen,<country>Denmark</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff10">Trace and Environmental DNA Laboratory, Department of Environment and Agriculture,<institution>Curtin University</institution>, Perth, 6102 WA,<country>Australia</country>;</nlm:aff></affiliation><affiliation><nlm:aff id="aff11"><institution>Norwegian University of Science and Technology (NTNU) University Museum</institution>, N-7491 Trondheim,<country>Norway</country></nlm:aff></affiliation></author><author><name sortKey="Lalueza Fox, Carles" sort="Lalueza Fox, Carles" uniqKey="Lalueza Fox C" first="Carles" last="Lalueza-Fox">Carles Lalueza-Fox</name><affiliation><nlm:aff id="aff1"><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>Previous studies have attempted to unravel the complex phylogeographic patterns of the malaria parasites <italic>Plasmodium vivax</italic> and <italic>Plasmidium falciparum</italic> diversity and also to understand its evolutionary affinities. However, all these studies are constrained by the lack of evidence from the eradicated European strains that could be central to some dispersals. This study successfully retrieves massive genetic data from old slides treated with traditional staining techniques to be observed under the microscope, thus providing a new material source for the study of past pathogens that could place value in historical medical collections. We generated sequence data from the eradicated European malaria parasites and shed light on the genetic diversity patterns of <italic>P. vivax</italic> and <italic>P. falciparum</italic>.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="iso-abbrev">Proc. Natl. Acad. Sci. U.S.A</journal-id><journal-id journal-id-type="hwp">pnas</journal-id><journal-id journal-id-type="pmc">pnas</journal-id><journal-id journal-id-type="publisher-id">PNAS</journal-id><journal-title-group><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title></journal-title-group><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27671660</article-id><article-id pub-id-type="pmc">5068322</article-id><article-id pub-id-type="publisher-id">201611017</article-id><article-id pub-id-type="doi">10.1073/pnas.1611017113</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Anthropology</subject></subj-group></subj-group><series-title>From the Cover</series-title></article-categories><title-group><article-title>Mitochondrial DNA from the eradicated European <italic>Plasmodium vivax</italic> and <italic>P. falciparum</italic> from 70-year-old slides from the Ebro Delta in Spain</article-title><alt-title alt-title-type="short">DNA from the eradicated European malaria parasites</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Gelabert</surname><given-names>Pere</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sandoval-Velasco</surname><given-names>Marcela</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Olalde</surname><given-names>Iñigo</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fregel</surname><given-names>Rosa</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Rieux</surname><given-names>Adrien</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Escosa</surname><given-names>Raül</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><contrib contrib-type="author"><name><surname>Aranda</surname><given-names>Carles</given-names></name><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Paaijmans</surname><given-names>Krijn</given-names></name><xref ref-type="aff" rid="aff7"><sup>g</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mueller</surname><given-names>Ivo</given-names></name><xref ref-type="aff" rid="aff7"><sup>g</sup></xref><xref ref-type="aff" rid="aff8"><sup>h</sup></xref><xref ref-type="aff" rid="aff9"><sup>i</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gilbert</surname><given-names>M. Thomas P.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="aff" rid="aff10"><sup>j</sup></xref><xref ref-type="aff" rid="aff11"><sup>k</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lalueza-Fox</surname><given-names>Carles</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1"><sup>a</sup><institution>Institute of Evolutionary Biology (Consejo Superior de Investigaciones Científicas-Universitat Pompeu Fabra)</institution>, 08003 Barcelona,<country>Spain</country>;</aff><aff id="aff2"><sup>b</sup>EvoGenomics, Natural History Museum of Denmark,<institution>University of Copenhagen</institution>, 1350 Copenhagen,<country>Denmark</country>;</aff><aff id="aff3"><sup>c</sup>Department of Genetics,<institution>Stanford University</institution>, Stanford,<addr-line>CA</addr-line> 94305;</aff><aff id="aff4"><sup>d</sup>Agricultural Research for Development (CIRAD), Systèmes biologiques (BIOS) Department,<institution>Peuplements végétaux et bioagresseurs en milieu tropical (PVBMT) Laboratory</institution>, 97410 St. Pierre de la Réunion,<country>France</country>;</aff><aff id="aff5"><sup>e</sup><institution>Consorci de Polítiques Ambientals de les Terres de l’Ebre</institution>, 43580 Deltebre,<country>Spain</country>;</aff><aff id="aff6"><sup>f</sup>Servei de Control de Mosquits,<institution>Consell Comarcal del Baix Llobregat</institution>, 08980 Sant Feliu de Llobregat,<country>Spain</country>;</aff><aff id="aff7"><sup>g</sup>ISGlobal, Barcelona Institute for Global Health,<institution>Hospital Clínic-Universitat de Barcelona</institution>, 08036 Barcelona,<country>Spain</country>;</aff><aff id="aff8"><sup>h</sup>Population Health and Immunity Division,<institution>Walter & Eliza Hall Institute</institution>, Parkville, 3052 VIC,<country>Australia</country>;</aff><aff id="aff9"><sup>i</sup>Department of Medical Biology,<institution>University of Melbourne</institution>, Parkville, 3052 VIC,<country>Australia</country>;</aff><aff id="aff10"><sup>j</sup>Trace and Environmental DNA Laboratory, Department of Environment and Agriculture,<institution>Curtin University</institution>, Perth, 6102 WA,<country>Australia</country>;</aff><aff id="aff11"><sup>k</sup><institution>Norwegian University of Science and Technology (NTNU) University Museum</institution>, N-7491 Trondheim,<country>Norway</country></aff></contrib-group><author-notes><corresp id="cor1"><sup>1</sup>To whom correspondence should be addressed Email: <email>carles.lalueza@upf.edu</email>.</corresp><fn fn-type="edited-by"><p>Edited by Richard G. Klein, Stanford University, Stanford, CA, and approved August 12, 2016 (received for review July 6, 2016)</p></fn><fn fn-type="con"><p>Author contributions: M.T.P.G. and C.L.-F. designed research; P.G., M.S.-V., R.F., I.M., and C.L.-F. performed research; R.E., C.A., K.P., and M.T.P.G. contributed new reagents/analytic tools; P.G., I.O., and A.R. analyzed data; and C.L.-F. wrote the paper with contributions from all authors.</p></fn></author-notes><pub-date pub-type="ppub"><day>11</day><month>10</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>26</day><month>9</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>26</day><month>9</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>113</volume><issue>41</issue><fpage>11495</fpage><lpage>11500</lpage><permissions><license license-type="open-access"><license-p>Freely available online through the PNAS open access option.</license-p></license></permissions><self-uri xlink:title="pdf" xlink:href="pnas.201611017.pdf"></self-uri><abstract abstract-type="executive-summary"><title>Significance</title><p>Previous studies have attempted to unravel the complex phylogeographic patterns of the malaria parasites <italic>Plasmodium vivax</italic> and <italic>Plasmidium falciparum</italic> diversity and also to understand its evolutionary affinities. However, all these studies are constrained by the lack of evidence from the eradicated European strains that could be central to some dispersals. This study successfully retrieves massive genetic data from old slides treated with traditional staining techniques to be observed under the microscope, thus providing a new material source for the study of past pathogens that could place value in historical medical collections. We generated sequence data from the eradicated European malaria parasites and shed light on the genetic diversity patterns of <italic>P. vivax</italic> and <italic>P. falciparum</italic>.</p></abstract><abstract><p>Phylogenetic analysis of <italic>Plasmodium</italic> parasites has indicated that their modern-day distribution is a result of a series of human-mediated dispersals involving transport between Africa, Europe, America, and Asia. A major outstanding question is the phylogenetic affinity of the malaria causing parasites <italic>Plasmodium vivax</italic> and <italic>falciparum</italic> in historic southern Europe—where it was endemic until the mid-20th century, after which it was eradicated across the region. Resolving the identity of these parasites will be critical for answering several hypotheses on the malaria dispersal. Recently, a set of slides with blood stains of malaria-affected people from the Ebro Delta (Spain), dated between 1942 and 1944, have been found in a local medical collection. We extracted DNA from three slides, two of them stained with Giemsa (on which <italic>Plasmodium</italic> parasites could still be seen under the microscope) and another one consisting of dried blood spots. We generated the data using Illumina sequencing after using several strategies aimed at increasing the <italic>Plasmodium</italic> DNA yield: depletion of the human genomic (g)DNA content through hybridization with human gDNA baits, and capture-enrichment using gDNA derived from <italic>P. falciparum</italic>. <italic>Plasmodium</italic> mitochondrial genome sequences were subsequently reconstructed from the resulting data. Phylogenetic analysis of the eradicated European <italic>P. vivax</italic> mtDNA genome indicates that the European isolate is closely related to the most common present-day American haplotype and likely entered the American continent post-Columbian contact. Furthermore, the European <italic>P. falciparum</italic> mtDNA indicates a link with current Indian strains that is in agreement with historical accounts.</p></abstract><kwd-group><kwd>malaria</kwd><kwd>Europe</kwd><kwd><italic>Plasmodium</italic></kwd><kwd>paleogenetics</kwd><kwd>microscopy slides</kwd></kwd-group><funding-group><award-group id="gs1"><funding-source id="sp1">FEDER and MINECO of Spain</funding-source><award-id rid="sp1">BFU2015-64699-P</award-id></award-group><award-group id="gs2"><funding-source id="sp2">European Research Council</funding-source><award-id rid="sp2">681396</award-id></award-group></funding-group><counts><page-count count="6"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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