A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 21[OPEN]
Identifieur interne : 001A90 ( Pmc/Corpus ); précédent : 001A89; suivant : 001A91A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 21[OPEN]
Auteurs : Christelle Taochy ; Nial R. Gursanscky ; Jiangling Cao ; Stephen J. Fletcher ; Uwe Dressel ; Neena Mitter ; Matthew R. Tucker ; Anna M. G. Koltunow ; John L. Bowman ; Hervé Vaucheret ; Bernard J. CarrollSource :
- Plant Physiology [ 0032-0889 ] ; 2017.
Abstract
DCL2 is required for efficient RDR6-dependent systemic posttranslational gene silencing, recruiting RNA-DEPENDENT RNA POLYMERASE6 and promoting the production of dsRNA that is mainly processed into 21-nucleotide siRNAs by DCL4 in wild-type plants.
Url:
DOI: 10.1104/pp.17.01181
PubMed: 28928141
PubMed Central: 5664484
Links to Exploration step
PMC:5664484Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 2<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Taochy, Christelle" sort="Taochy, Christelle" uniqKey="Taochy C" first="Christelle" last="Taochy">Christelle Taochy</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique AgroParisTech Centre National de la Recherche Scientifique, Université Paris-Saclay, 78000 Versailles, France</nlm:aff></affiliation></author><author><name sortKey="Gursanscky, Nial R" sort="Gursanscky, Nial R" uniqKey="Gursanscky N" first="Nial R." last="Gursanscky">Nial R. Gursanscky</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Cao, Jiangling" sort="Cao, Jiangling" uniqKey="Cao J" first="Jiangling" last="Cao">Jiangling Cao</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Fletcher, Stephen J" sort="Fletcher, Stephen J" uniqKey="Fletcher S" first="Stephen J." last="Fletcher">Stephen J. Fletcher</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Dressel, Uwe" sort="Dressel, Uwe" uniqKey="Dressel U" first="Uwe" last="Dressel">Uwe Dressel</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Mitter, Neena" sort="Mitter, Neena" uniqKey="Mitter N" first="Neena" last="Mitter">Neena Mitter</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Tucker, Matthew R" sort="Tucker, Matthew R" uniqKey="Tucker M" first="Matthew R." last="Tucker">Matthew R. Tucker</name><affiliation><nlm:aff id="aff4">Commonwealth Scientific and Industrial Research Organization Agriculture, Waite Campus, Urrbrae, South Australia 5064, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Adelaide, South Australia 5064, Australia</nlm:aff></affiliation></author><author><name sortKey="Koltunow, Anna M G" sort="Koltunow, Anna M G" uniqKey="Koltunow A" first="Anna M. G." last="Koltunow">Anna M. G. Koltunow</name><affiliation><nlm:aff id="aff4">Commonwealth Scientific and Industrial Research Organization Agriculture, Waite Campus, Urrbrae, South Australia 5064, Australia</nlm:aff></affiliation></author><author><name sortKey="Bowman, John L" sort="Bowman, John L" uniqKey="Bowman J" first="John L." last="Bowman">John L. Bowman</name><affiliation><nlm:aff id="aff6">School of Biological Sciences, Monash University, Clayton Campus, Melbourne, Victoria 3800, Australia</nlm:aff></affiliation></author><author><name sortKey="Vaucheret, Herve" sort="Vaucheret, Herve" uniqKey="Vaucheret H" first="Hervé" last="Vaucheret">Hervé Vaucheret</name><affiliation><nlm:aff id="aff2">Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique AgroParisTech Centre National de la Recherche Scientifique, Université Paris-Saclay, 78000 Versailles, France</nlm:aff></affiliation></author><author><name sortKey="Carroll, Bernard J" sort="Carroll, Bernard J" uniqKey="Carroll B" first="Bernard J." last="Carroll">Bernard J. Carroll</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28928141</idno><idno type="pmc">5664484</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664484</idno><idno type="RBID">PMC:5664484</idno><idno type="doi">10.1104/pp.17.01181</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">001A90</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001A90</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 2<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Taochy, Christelle" sort="Taochy, Christelle" uniqKey="Taochy C" first="Christelle" last="Taochy">Christelle Taochy</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique AgroParisTech Centre National de la Recherche Scientifique, Université Paris-Saclay, 78000 Versailles, France</nlm:aff></affiliation></author><author><name sortKey="Gursanscky, Nial R" sort="Gursanscky, Nial R" uniqKey="Gursanscky N" first="Nial R." last="Gursanscky">Nial R. Gursanscky</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Cao, Jiangling" sort="Cao, Jiangling" uniqKey="Cao J" first="Jiangling" last="Cao">Jiangling Cao</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Fletcher, Stephen J" sort="Fletcher, Stephen J" uniqKey="Fletcher S" first="Stephen J." last="Fletcher">Stephen J. Fletcher</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Dressel, Uwe" sort="Dressel, Uwe" uniqKey="Dressel U" first="Uwe" last="Dressel">Uwe Dressel</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Mitter, Neena" sort="Mitter, Neena" uniqKey="Mitter N" first="Neena" last="Mitter">Neena Mitter</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Tucker, Matthew R" sort="Tucker, Matthew R" uniqKey="Tucker M" first="Matthew R." last="Tucker">Matthew R. Tucker</name><affiliation><nlm:aff id="aff4">Commonwealth Scientific and Industrial Research Organization Agriculture, Waite Campus, Urrbrae, South Australia 5064, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Adelaide, South Australia 5064, Australia</nlm:aff></affiliation></author><author><name sortKey="Koltunow, Anna M G" sort="Koltunow, Anna M G" uniqKey="Koltunow A" first="Anna M. G." last="Koltunow">Anna M. G. Koltunow</name><affiliation><nlm:aff id="aff4">Commonwealth Scientific and Industrial Research Organization Agriculture, Waite Campus, Urrbrae, South Australia 5064, Australia</nlm:aff></affiliation></author><author><name sortKey="Bowman, John L" sort="Bowman, John L" uniqKey="Bowman J" first="John L." last="Bowman">John L. Bowman</name><affiliation><nlm:aff id="aff6">School of Biological Sciences, Monash University, Clayton Campus, Melbourne, Victoria 3800, Australia</nlm:aff></affiliation></author><author><name sortKey="Vaucheret, Herve" sort="Vaucheret, Herve" uniqKey="Vaucheret H" first="Hervé" last="Vaucheret">Hervé Vaucheret</name><affiliation><nlm:aff id="aff2">Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique AgroParisTech Centre National de la Recherche Scientifique, Université Paris-Saclay, 78000 Versailles, France</nlm:aff></affiliation></author><author><name sortKey="Carroll, Bernard J" sort="Carroll, Bernard J" uniqKey="Carroll B" first="Bernard J." last="Carroll">Bernard J. Carroll</name><affiliation><nlm:aff id="aff1">School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff></affiliation></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>DCL2 is required for efficient RDR6-dependent systemic posttranslational gene silencing, recruiting RNA-DEPENDENT RNA POLYMERASE6 and promoting the production of dsRNA that is mainly processed into 21-nucleotide siRNAs by DCL4 in wild-type plants.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28928141</article-id><article-id pub-id-type="pmc">5664484</article-id><article-id pub-id-type="publisher-id">PP2017RA01181D</article-id><article-id pub-id-type="doi">10.1104/pp.17.01181</article-id><article-categories><subj-group><subject>Articles</subject></subj-group><subj-group subj-group-type="heading"><subject>Signaling and Response</subject></subj-group></article-categories><title-group><article-title>A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 2<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Taochy</surname><given-names>Christelle</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0002-4221-570X</contrib-id><name><surname>Gursanscky</surname><given-names>Nial R.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cao</surname><given-names>Jiangling</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fletcher</surname><given-names>Stephen J.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-4505-7855</contrib-id><name><surname>Dressel</surname><given-names>Uwe</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mitter</surname><given-names>Neena</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-4661-6700</contrib-id><name><surname>Tucker</surname><given-names>Matthew R.</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0001-5016-7683</contrib-id><name><surname>Koltunow</surname><given-names>Anna M.G.</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0001-7347-3691</contrib-id><name><surname>Bowman</surname><given-names>John L.</given-names></name><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Vaucheret</surname><given-names>Hervé</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0002-1976-8269</contrib-id><name><surname>Carroll</surname><given-names>Bernard J.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="corresp" rid="cor1"><sup>2</sup></xref></contrib><aff id="aff1"><label>a</label>School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia</aff><aff id="aff2"><label>b</label>Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique AgroParisTech Centre National de la Recherche Scientifique, Université Paris-Saclay, 78000 Versailles, France</aff><aff id="aff3"><label>c</label>Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia</aff><aff id="aff4"><label>d</label>Commonwealth Scientific and Industrial Research Organization Agriculture, Waite Campus, Urrbrae, South Australia 5064, Australia</aff><aff id="aff5"><label>e</label>School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Adelaide, South Australia 5064, Australia</aff><aff id="aff6"><label>f</label>School of Biological Sciences, Monash University, Clayton Campus, Melbourne, Victoria 3800, Australia</aff></contrib-group><author-notes><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org/cgi/doi/10.1104/pp.17.01181">www.plantphysiol.org/cgi/doi/10.1104/pp.17.01181</ext-link></p></fn><corresp id="cor1"><label>2</label>Address correspondence to <email>b.carroll@uq.edu.au</email>.</corresp><fn id="afn1"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Bernard J. Carroll (<email>b.carroll@uq.edu.au</email>).</p></fn><fn id="afn2"><p>C.T., N.R.G., M.R.T., A.M.G.K., J.L.B., H.V., and B.J.C. designed the research; C.T., J.C., N.R.G., U.D., N.M., and M.R.T. performed the research; C.T., J.C., N.R.G., S.J.F., U.D., H.V., and B.J.C. analyzed the data; C.T., N.R.G., H.V., and B.J.C. wrote the article.</p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>11</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>19</day><month>9</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>19</day><month>9</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>175</volume><issue>3</issue><fpage>1424</fpage><lpage>1437</lpage><history><date date-type="received"><day>28</day><month>8</month><year>2017</year></date><date date-type="accepted"><day>14</day><month>9</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 American Society of Plant Biologists. All Rights Reserved.</copyright-statement><copyright-year>2017</copyright-year></permissions><self-uri xlink:role="icon" xlink:href="PP_PP2017RA01181D_ic1.gif"></self-uri><abstract abstract-type="precis"><p>DCL2 is required for efficient RDR6-dependent systemic posttranslational gene silencing, recruiting RNA-DEPENDENT RNA POLYMERASE6 and promoting the production of dsRNA that is mainly processed into 21-nucleotide siRNAs by DCL4 in wild-type plants.</p></abstract><abstract><p>Posttranscriptional gene silencing (PTGS) of transgenes involves abundant 21-nucleotide small interfering RNAs (siRNAs) and low-abundance 22-nucleotide siRNAs produced from double-stranded RNA (dsRNA) by DCL4 and DCL2, respectively. However, DCL2 facilitates the recruitment of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) to ARGONAUTE 1-derived cleavage products, resulting in more efficient amplification of secondary and transitive dsRNA and siRNAs. Here, we describe a reporter system where RDR6-dependent PTGS is initiated by restricted expression of an inverted-repeat dsRNA specifically in the Arabidopsis (<italic>Arabidopsis thaliana</italic>) root tip, allowing a genetic screen to identify mutants impaired in RDR6-dependent systemic PTGS. Our screen identified <italic>dcl2</italic> but not <italic>dcl4</italic> mutants. Moreover, grafting experiments showed that DCL2, but not DCL4, is required in both the source rootstock and the recipient shoot tissue for efficient RDR6-dependent systemic PTGS. Furthermore, <italic>dcl4</italic> rootstocks produced more DCL2-dependent 22-nucleotide siRNAs than the wild type and showed enhanced systemic movement of PTGS to grafted shoots. Thus, along with its role in recruiting RDR6 for further amplification of PTGS, DCL2 is crucial for RDR6-dependent systemic PTGS.</p></abstract><funding-group><award-group id="sp1"><funding-source>ARC</funding-source></award-group><award-group id="sp2"><funding-source>ANR</funding-source></award-group><award-group id="sp3"><funding-source>Fondation Louis D.</funding-source></award-group><award-group id="sp4"><funding-source>CJS-INRA</funding-source></award-group><award-group id="sp5"><funding-source>French Agence Nationale pour la Recherche</funding-source><award-id rid="sp5">ANR-10-LABX-40</award-id><award-id rid="sp5">ANR-11-BSV6-007</award-id></award-group><award-group id="sp6"><funding-source>GRDC postgraduate research scholarship</funding-source><award-id rid="sp6">GRS114</award-id></award-group><award-group id="sp7"><funding-source>Australian Research Council</funding-source><award-id rid="sp7">DP0988294</award-id><award-id rid="sp7">DP120103966</award-id><award-id rid="sp7">DP150104048</award-id></award-group></funding-group><counts><fig-count count="6"></fig-count><table-count count="0"></table-count><equation-count count="0"></equation-count><ref-count count="55"></ref-count><page-count count="14"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A90 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001A90 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:5664484
|texte= A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 21[OPEN]
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:28928141" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |