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<title xml:lang="en">Perturbation of Indole-3-Butyric Acid Homeostasis by the UDP-Glucosyltransferase
<italic>UGT74E2</italic>
Modulates
<italic>Arabidopsis</italic>
Architecture and Water Stress Tolerance
<xref ref-type="author-notes" rid="fn3">
<sup>[W]</sup>
</xref>
</title>
<author>
<name sortKey="Tognetti, Vanesa B" sort="Tognetti, Vanesa B" uniqKey="Tognetti V" first="Vanesa B." last="Tognetti">Vanesa B. Tognetti</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Van Aken, Olivier" sort="Van Aken, Olivier" uniqKey="Van Aken O" first="Olivier" last="Van Aken">Olivier Van Aken</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff3">ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Morreel, Kris" sort="Morreel, Kris" uniqKey="Morreel K" first="Kris" last="Morreel">Kris Morreel</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Vandenbroucke, Korneel" sort="Vandenbroucke, Korneel" uniqKey="Vandenbroucke K" first="Korneel" last="Vandenbroucke">Korneel Vandenbroucke</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Van De Cotte, Brigitte" sort="Van De Cotte, Brigitte" uniqKey="Van De Cotte B" first="Brigitte" last="Van De Cotte">Brigitte Van De Cotte</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="De Clercq, Inge" sort="De Clercq, Inge" uniqKey="De Clercq I" first="Inge" last="De Clercq">Inge De Clercq</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Chiwocha, Sheila" sort="Chiwocha, Sheila" uniqKey="Chiwocha S" first="Sheila" last="Chiwocha">Sheila Chiwocha</name>
<affiliation>
<nlm:aff id="aff3">ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Fenske, Ricarda" sort="Fenske, Ricarda" uniqKey="Fenske R" first="Ricarda" last="Fenske">Ricarda Fenske</name>
<affiliation>
<nlm:aff id="aff3">ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Prinsen, Els" sort="Prinsen, Els" uniqKey="Prinsen E" first="Els" last="Prinsen">Els Prinsen</name>
<affiliation>
<nlm:aff id="aff4">Departement Biologie, Universiteit Antwerpen, 2020 Antwerpen, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Genty, Bernard" sort="Genty, Bernard" uniqKey="Genty B" first="Bernard" last="Genty">Bernard Genty</name>
<affiliation>
<nlm:aff id="aff5">Centre d'Etudes Atomiques, Centre National de la Recherche Scientifique, Université Aix-Marseille, Unité Mixte de Recherche 6191 Biologie Végétale et Microbiologie Environnementale, Laboratoire d'Ecophysiologie Moléculaire des Plantes, 13108 Saint Paul lez Durance, France</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Stubbs, Keith A" sort="Stubbs, Keith A" uniqKey="Stubbs K" first="Keith A." last="Stubbs">Keith A. Stubbs</name>
<affiliation>
<nlm:aff id="aff6">School of Biomedical, Biomolecular, and Chemical Sciences, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Inze, Dirk" sort="Inze, Dirk" uniqKey="Inze D" first="Dirk" last="Inzé">Dirk Inzé</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Van Breusegem, Frank" sort="Van Breusegem, Frank" uniqKey="Van Breusegem F" first="Frank" last="Van Breusegem">Frank Van Breusegem</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
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<idno type="pmid">20798329</idno>
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<idno type="doi">10.1105/tpc.109.071316</idno>
<date when="2010">2010</date>
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<title xml:lang="en" level="a" type="main">Perturbation of Indole-3-Butyric Acid Homeostasis by the UDP-Glucosyltransferase
<italic>UGT74E2</italic>
Modulates
<italic>Arabidopsis</italic>
Architecture and Water Stress Tolerance
<xref ref-type="author-notes" rid="fn3">
<sup>[W]</sup>
</xref>
</title>
<author>
<name sortKey="Tognetti, Vanesa B" sort="Tognetti, Vanesa B" uniqKey="Tognetti V" first="Vanesa B." last="Tognetti">Vanesa B. Tognetti</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Van Aken, Olivier" sort="Van Aken, Olivier" uniqKey="Van Aken O" first="Olivier" last="Van Aken">Olivier Van Aken</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff3">ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Morreel, Kris" sort="Morreel, Kris" uniqKey="Morreel K" first="Kris" last="Morreel">Kris Morreel</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Vandenbroucke, Korneel" sort="Vandenbroucke, Korneel" uniqKey="Vandenbroucke K" first="Korneel" last="Vandenbroucke">Korneel Vandenbroucke</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Van De Cotte, Brigitte" sort="Van De Cotte, Brigitte" uniqKey="Van De Cotte B" first="Brigitte" last="Van De Cotte">Brigitte Van De Cotte</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="De Clercq, Inge" sort="De Clercq, Inge" uniqKey="De Clercq I" first="Inge" last="De Clercq">Inge De Clercq</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Chiwocha, Sheila" sort="Chiwocha, Sheila" uniqKey="Chiwocha S" first="Sheila" last="Chiwocha">Sheila Chiwocha</name>
<affiliation>
<nlm:aff id="aff3">ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Fenske, Ricarda" sort="Fenske, Ricarda" uniqKey="Fenske R" first="Ricarda" last="Fenske">Ricarda Fenske</name>
<affiliation>
<nlm:aff id="aff3">ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Prinsen, Els" sort="Prinsen, Els" uniqKey="Prinsen E" first="Els" last="Prinsen">Els Prinsen</name>
<affiliation>
<nlm:aff id="aff4">Departement Biologie, Universiteit Antwerpen, 2020 Antwerpen, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Genty, Bernard" sort="Genty, Bernard" uniqKey="Genty B" first="Bernard" last="Genty">Bernard Genty</name>
<affiliation>
<nlm:aff id="aff5">Centre d'Etudes Atomiques, Centre National de la Recherche Scientifique, Université Aix-Marseille, Unité Mixte de Recherche 6191 Biologie Végétale et Microbiologie Environnementale, Laboratoire d'Ecophysiologie Moléculaire des Plantes, 13108 Saint Paul lez Durance, France</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Stubbs, Keith A" sort="Stubbs, Keith A" uniqKey="Stubbs K" first="Keith A." last="Stubbs">Keith A. Stubbs</name>
<affiliation>
<nlm:aff id="aff6">School of Biomedical, Biomolecular, and Chemical Sciences, University of Western Australia, Perth 6009, Australia</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Inze, Dirk" sort="Inze, Dirk" uniqKey="Inze D" first="Dirk" last="Inzé">Dirk Inzé</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
<author>
<name sortKey="Van Breusegem, Frank" sort="Van Breusegem, Frank" uniqKey="Van Breusegem F" first="Frank" last="Van Breusegem">Frank Van Breusegem</name>
<affiliation>
<nlm:aff id="aff1">Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
<affiliation>
<nlm:aff id="aff2">Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</nlm:aff>
</affiliation>
</author>
</analytic>
<series>
<title level="j">The Plant Cell</title>
<idno type="ISSN">1040-4651</idno>
<idno type="eISSN">1532-298X</idno>
<imprint>
<date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
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</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">
<p>The hydrogen peroxide–responsive UDP-glucosyltransferase UGT74E2 from
<italic>Arabidopsis thaliana</italic>
is shown to be involved in modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Evidence is provided that, during water stress, IBA and IBA-glucose levels increase, and auxins help maintain the photosynthetic capacity under stress.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article">
<pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">Plant Cell</journal-id>
<journal-id journal-id-type="hwp">plantcell</journal-id>
<journal-id journal-id-type="publisher-id">aspb</journal-id>
<journal-title-group>
<journal-title>The Plant Cell</journal-title>
</journal-title-group>
<issn pub-type="ppub">1040-4651</issn>
<issn pub-type="epub">1532-298X</issn>
<publisher>
<publisher-name>American Society of Plant Biologists</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="pmid">20798329</article-id>
<article-id pub-id-type="pmc">2947170</article-id>
<article-id pub-id-type="publisher-id">071316</article-id>
<article-id pub-id-type="doi">10.1105/tpc.109.071316</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Research Articles</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Perturbation of Indole-3-Butyric Acid Homeostasis by the UDP-Glucosyltransferase
<italic>UGT74E2</italic>
Modulates
<italic>Arabidopsis</italic>
Architecture and Water Stress Tolerance
<xref ref-type="author-notes" rid="fn3">
<sup>[W]</sup>
</xref>
</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Tognetti</surname>
<given-names>Vanesa B.</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Van Aken</surname>
<given-names>Olivier</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
<xref ref-type="aff" rid="aff3">
<sup>c</sup>
</xref>
<xref ref-type="author-notes" rid="fn1">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Morreel</surname>
<given-names>Kris</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Vandenbroucke</surname>
<given-names>Korneel</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
<xref ref-type="author-notes" rid="fn2">
<sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>van de Cotte</surname>
<given-names>Brigitte</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>De Clercq</surname>
<given-names>Inge</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Chiwocha</surname>
<given-names>Sheila</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Fenske</surname>
<given-names>Ricarda</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Prinsen</surname>
<given-names>Els</given-names>
</name>
<xref ref-type="aff" rid="aff4">
<sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Boerjan</surname>
<given-names>Wout</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Genty</surname>
<given-names>Bernard</given-names>
</name>
<xref ref-type="aff" rid="aff5">
<sup>e</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Stubbs</surname>
<given-names>Keith A.</given-names>
</name>
<xref ref-type="aff" rid="aff6">
<sup>f</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Inzé</surname>
<given-names>Dirk</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Van Breusegem</surname>
<given-names>Frank</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>b</sup>
</xref>
<xref ref-type="corresp" rid="cor1">
<sup>3</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1">
<label>a</label>
Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium</aff>
<aff id="aff2">
<label>b</label>
Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium</aff>
<aff id="aff3">
<label>c</label>
ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Perth 6009, Australia</aff>
<aff id="aff4">
<label>d</label>
Departement Biologie, Universiteit Antwerpen, 2020 Antwerpen, Belgium</aff>
<aff id="aff5">
<label>e</label>
Centre d'Etudes Atomiques, Centre National de la Recherche Scientifique, Université Aix-Marseille, Unité Mixte de Recherche 6191 Biologie Végétale et Microbiologie Environnementale, Laboratoire d'Ecophysiologie Moléculaire des Plantes, 13108 Saint Paul lez Durance, France</aff>
<aff id="aff6">
<label>f</label>
School of Biomedical, Biomolecular, and Chemical Sciences, University of Western Australia, Perth 6009, Australia</aff>
<author-notes>
<corresp id="cor1">
<label>3</label>
Address correspondence to
<email>frank.vanbreusegem@psb.vib-ugent.be</email>
.</corresp>
<fn id="fn1" fn-type="equal">
<label>1</label>
<p>These authors contributed equally to this work.</p>
</fn>
<fn id="fn2" fn-type="present-address">
<label>2</label>
<p>Current address: Bayer BioScience, Technologiepark 38, B-9052 Gent, Belgium.</p>
</fn>
<fn>
<p>The author responsible for distribution of materials integral to the findings presented in the article in accordance with the policy described in the Instructions for Authors (
<ext-link ext-link-type="uri" xlink:href="www.plantcell.org">www.plantcell.org</ext-link>
) is: Frank Van Breusegem (
<email>frank.vanbreusegem@psb.vib-ugent.be</email>
).</p>
</fn>
<fn id="fn3">
<label>[W]</label>
<p>Online version contains Web-only data.</p>
</fn>
<fn>
<p>
<ext-link ext-link-type="uri" xlink:href="www.plantcell.org/cgi/doi/10.1105/tpc.109.071316">www.plantcell.org/cgi/doi/10.1105/tpc.109.071316</ext-link>
</p>
</fn>
</author-notes>
<pmc-comment>Fake ppub date generated by PMC from publisher pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub">
<month>8</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub">
<day>26</day>
<month>8</month>
<year>2010</year>
</pub-date>
<volume>22</volume>
<issue>8</issue>
<fpage>2660</fpage>
<lpage>2679</lpage>
<history>
<date date-type="received">
<day>11</day>
<month>9</month>
<year>2009</year>
</date>
<date date-type="rev-recd">
<day>16</day>
<month>7</month>
<year>2010</year>
</date>
<date date-type="accepted">
<day>5</day>
<month>8</month>
<year>2010</year>
</date>
</history>
<permissions>
<copyright-statement>© 2010 American Society of Plant Biologists</copyright-statement>
<copyright-year>2010</copyright-year>
</permissions>
<abstract abstract-type="precis">
<p>The hydrogen peroxide–responsive UDP-glucosyltransferase UGT74E2 from
<italic>Arabidopsis thaliana</italic>
is shown to be involved in modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Evidence is provided that, during water stress, IBA and IBA-glucose levels increase, and auxins help maintain the photosynthetic capacity under stress.</p>
</abstract>
<abstract>
<p>Reactive oxygen species and redox signaling undergo synergistic and antagonistic interactions with phytohormones to regulate protective responses of plants against biotic and abiotic stresses. However, molecular insight into the nature of this crosstalk remains scarce. We demonstrate that the hydrogen peroxide–responsive UDP-glucosyltransferase UGT74E2 of
<italic>Arabidopsis thaliana</italic>
is involved in the modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Biochemical characterization of recombinant UGT74E2 demonstrated that it strongly favors IBA as a substrate. Assessment of indole-3-acetic acid (IAA), IBA, and their conjugates in transgenic plants ectopically expressing
<italic>UGT74E2</italic>
indicated that the catalytic specificity was maintained in planta. In these transgenic plants, not only were IBA-Glc concentrations increased, but also free IBA levels were elevated and the conjugated IAA pattern was modified. This perturbed IBA and IAA homeostasis was associated with architectural changes, including increased shoot branching and altered rosette shape, and resulted in significantly improved survival during drought and salt stress treatments. Hence, our results reveal that IBA and IBA-Glc are important regulators of morphological and physiological stress adaptation mechanisms and provide molecular evidence for the interplay between hydrogen peroxide and auxin homeostasis through the action of an IBA UGT.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>

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