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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response</title><author><name sortKey="Jones, Jana" sort="Jones, Jana" uniqKey="Jones J" first="Jana" last="Jones">Jana Jones</name><affiliation><nlm:aff id="af1"><addr-line>Department of Ancient History</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Mirzaei, Mehdi" sort="Mirzaei, Mehdi" uniqKey="Mirzaei M" first="Mehdi" last="Mirzaei">Mehdi Mirzaei</name><affiliation><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Ravishankar, Prathiba" sort="Ravishankar, Prathiba" uniqKey="Ravishankar P" first="Prathiba" last="Ravishankar">Prathiba Ravishankar</name><affiliation><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Xavier, Dylan" sort="Xavier, Dylan" uniqKey="Xavier D" first="Dylan" last="Xavier">Dylan Xavier</name><affiliation><nlm:aff id="af3"><addr-line>Australian Proteome Analysis Facility</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do Seon" last="Lim">Do Seon Lim</name><affiliation><nlm:aff id="af4"><addr-line>Department of Dental Hygiene, College of Health Sciences</addr-line>,<institution>Eulji University</institution>,<addr-line>Sungnam</addr-line>,<country>South Korea</country></nlm:aff></affiliation></author><author><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name><affiliation><nlm:aff id="af5"><addr-line>Department of Anatomy</addr-line>,<institution>Seoul National University, College of Medicine</institution>,<addr-line>Seoul</addr-line>,<country>South Korea</country></nlm:aff></affiliation></author><author><name sortKey="Bianucci, Raffaella" sort="Bianucci, Raffaella" uniqKey="Bianucci R" first="Raffaella" last="Bianucci">Raffaella Bianucci</name><affiliation><nlm:aff id="af6"><addr-line>Department of Public Health and Paediatric Sciences, Legal Medicine Section</addr-line>,<institution>University of Turin</institution>,<addr-line>10126 Turin</addr-line>,<country>Italy</country></nlm:aff></affiliation><affiliation><nlm:aff id="af7"><institution>UMR 7268, Laboratoire d'Anthropologie bio-culturelle, Droit, Étique and Santé (ADÉS), Faculté de Médecine de Marseille</institution>,<addr-line>13344 Marseille</addr-line>,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Haynes, Paul A" sort="Haynes, Paul A" uniqKey="Haynes P" first="Paul A." last="Haynes">Paul A. Haynes</name><affiliation><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27644972</idno><idno type="pmc">5031639</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031639</idno><idno type="RBID">PMC:5031639</idno><idno type="doi">10.1098/rsta.2015.0373</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">001A48</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001A48</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response</title><author><name sortKey="Jones, Jana" sort="Jones, Jana" uniqKey="Jones J" first="Jana" last="Jones">Jana Jones</name><affiliation><nlm:aff id="af1"><addr-line>Department of Ancient History</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Mirzaei, Mehdi" sort="Mirzaei, Mehdi" uniqKey="Mirzaei M" first="Mehdi" last="Mirzaei">Mehdi Mirzaei</name><affiliation><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Ravishankar, Prathiba" sort="Ravishankar, Prathiba" uniqKey="Ravishankar P" first="Prathiba" last="Ravishankar">Prathiba Ravishankar</name><affiliation><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Xavier, Dylan" sort="Xavier, Dylan" uniqKey="Xavier D" first="Dylan" last="Xavier">Dylan Xavier</name><affiliation><nlm:aff id="af3"><addr-line>Australian Proteome Analysis Facility</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do Seon" last="Lim">Do Seon Lim</name><affiliation><nlm:aff id="af4"><addr-line>Department of Dental Hygiene, College of Health Sciences</addr-line>,<institution>Eulji University</institution>,<addr-line>Sungnam</addr-line>,<country>South Korea</country></nlm:aff></affiliation></author><author><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name><affiliation><nlm:aff id="af5"><addr-line>Department of Anatomy</addr-line>,<institution>Seoul National University, College of Medicine</institution>,<addr-line>Seoul</addr-line>,<country>South Korea</country></nlm:aff></affiliation></author><author><name sortKey="Bianucci, Raffaella" sort="Bianucci, Raffaella" uniqKey="Bianucci R" first="Raffaella" last="Bianucci">Raffaella Bianucci</name><affiliation><nlm:aff id="af6"><addr-line>Department of Public Health and Paediatric Sciences, Legal Medicine Section</addr-line>,<institution>University of Turin</institution>,<addr-line>10126 Turin</addr-line>,<country>Italy</country></nlm:aff></affiliation><affiliation><nlm:aff id="af7"><institution>UMR 7268, Laboratoire d'Anthropologie bio-culturelle, Droit, Étique and Santé (ADÉS), Faculté de Médecine de Marseille</institution>,<addr-line>13344 Marseille</addr-line>,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Haynes, Paul A" sort="Haynes, Paul A" uniqKey="Haynes P" first="Paul A." last="Haynes">Paul A. Haynes</name><affiliation><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff></affiliation></author></analytic><series><title level="j">Philosophical transactions. Series A, Mathematical, physical, and engineering sciences</title><idno type="ISSN">1364-503X</idno><idno type="eISSN">1471-2962</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We performed proteomics analysis on four skin and one muscle tissue samples taken from three ancient Egyptian mummies of the first intermediate period, approximately 4200 years old. The mummies were first dated by radiocarbon dating of the accompany-\break ing textiles, and morphologically examined by scanning electron microscopy of additional skin samples. Proteins were extracted, separated on SDS–PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) gels, and in-gel digested with trypsin. The resulting peptides were analysed using nanoflow high-performance liquid chromatography–mass spectrometry. We identified a total of 230 unique proteins from the five samples, which consisted of 132 unique protein identifications. We found a large number of collagens, which was confirmed by our microscopy data, and is in agreement with previous studies showing that collagens are very long-lived. As expected, we also found a large number of keratins. We identified numerous proteins that provide evidence of activation of the innate immunity system in two of the mummies, one of which also contained proteins indicating severe tissue inflammation, possibly indicative of an infection that we can speculate may have been related to the cause of death.</p><p>This article is part of the themed issue ‘Quantitative mass spectrometry’.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Philos Trans A Math Phys Eng Sci</journal-id><journal-id journal-id-type="iso-abbrev">Philos Trans A Math Phys Eng Sci</journal-id><journal-id journal-id-type="publisher-id">RSTA</journal-id><journal-id journal-id-type="hwp">roypta</journal-id><journal-title-group><journal-title>Philosophical transactions. Series A, Mathematical, physical, and engineering sciences</journal-title></journal-title-group><issn pub-type="ppub">1364-503X</issn><issn pub-type="epub">1471-2962</issn><publisher><publisher-name>The Royal Society</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27644972</article-id><article-id pub-id-type="pmc">5031639</article-id><article-id pub-id-type="doi">10.1098/rsta.2015.0373</article-id><article-id pub-id-type="publisher-id">rsta20150373</article-id><article-categories><subj-group subj-group-type="hwp-journal-coll"><subject>1002</subject><subject>5</subject></subj-group><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="leader"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response</article-title><alt-title alt-title-type="short">Protein identification in Mummy tissues</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jones</surname><given-names>Jana</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="author-notes" rid="an1">†</xref></contrib><contrib contrib-type="author"><name><surname>Mirzaei</surname><given-names>Mehdi</given-names></name><xref ref-type="aff" rid="af2">2</xref><xref ref-type="author-notes" rid="an1">†</xref></contrib><contrib contrib-type="author"><name><surname>Ravishankar</surname><given-names>Prathiba</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Xavier</surname><given-names>Dylan</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Lim</surname><given-names>Do Seon</given-names></name><xref ref-type="aff" rid="af4">4</xref></contrib><contrib contrib-type="author"><name><surname>Shin</surname><given-names>Dong Hoon</given-names></name><xref ref-type="aff" rid="af5">5</xref></contrib><contrib contrib-type="author"><name><surname>Bianucci</surname><given-names>Raffaella</given-names></name><xref ref-type="aff" rid="af6">6</xref><xref ref-type="aff" rid="af7">7</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-1472-8249</contrib-id><name><surname>Haynes</surname><given-names>Paul A.</given-names></name><xref ref-type="aff" rid="af2">2</xref><xref ref-type="corresp" rid="cor1"></xref></contrib></contrib-group><aff id="af1"><label>1</label><addr-line>Department of Ancient History</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></aff><aff id="af2"><label>2</label><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></aff><aff id="af3"><label>3</label><addr-line>Australian Proteome Analysis Facility</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></aff><aff id="af4"><label>4</label><addr-line>Department of Dental Hygiene, College of Health Sciences</addr-line>,<institution>Eulji University</institution>,<addr-line>Sungnam</addr-line>,<country>South Korea</country></aff><aff id="af5"><label>5</label><addr-line>Department of Anatomy</addr-line>,<institution>Seoul National University, College of Medicine</institution>,<addr-line>Seoul</addr-line>,<country>South Korea</country></aff><aff id="af6"><label>6</label><addr-line>Department of Public Health and Paediatric Sciences, Legal Medicine Section</addr-line>,<institution>University of Turin</institution>,<addr-line>10126 Turin</addr-line>,<country>Italy</country></aff><aff id="af7"><label>7</label><institution>UMR 7268, Laboratoire d'Anthropologie bio-culturelle, Droit, Étique and Santé (ADÉS), Faculté de Médecine de Marseille</institution>,<addr-line>13344 Marseille</addr-line>,<country>France</country></aff><author-notes><corresp id="cor1">e-mail: <email>paul.haynes@mq.edu.au</email></corresp><fn id="an1" fn-type="con"><label>†</label><p>These authors contributed equally to this study.</p></fn><fn fn-type="other"><p>One contribution of 19 to a theme issue ‘<ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1098/rsta/374/2079">Quantitative mass spectrometry</ext-link>’.</p></fn></author-notes><pub-date pub-type="ppub"><day>28</day><month>10</month><year>2016</year></pub-date><volume>374</volume><issue>2079</issue><issue-title>Theme issue ‘Quantitative mass spectrometry’ compiled and edited by Pawel Urban</issue-title><elocation-id>20150373</elocation-id><history><date date-type="accepted"><day>28</day><month>6</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 The Author(s)</copyright-statement><copyright-year>2016</copyright-year><license specific-use="vor" xlink:href="http://royalsocietypublishing.org/licence"><license-p>Published by the Royal Society. All rights reserved.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="rsta20150373.pdf"></self-uri><abstract><p>We performed proteomics analysis on four skin and one muscle tissue samples taken from three ancient Egyptian mummies of the first intermediate period, approximately 4200 years old. The mummies were first dated by radiocarbon dating of the accompany-\break ing textiles, and morphologically examined by scanning electron microscopy of additional skin samples. Proteins were extracted, separated on SDS–PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) gels, and in-gel digested with trypsin. The resulting peptides were analysed using nanoflow high-performance liquid chromatography–mass spectrometry. We identified a total of 230 unique proteins from the five samples, which consisted of 132 unique protein identifications. We found a large number of collagens, which was confirmed by our microscopy data, and is in agreement with previous studies showing that collagens are very long-lived. As expected, we also found a large number of keratins. We identified numerous proteins that provide evidence of activation of the innate immunity system in two of the mummies, one of which also contained proteins indicating severe tissue inflammation, possibly indicative of an infection that we can speculate may have been related to the cause of death.</p><p>This article is part of the themed issue ‘Quantitative mass spectrometry’.</p></abstract><kwd-group><kwd>ancient proteins</kwd><kwd>protein identification</kwd><kwd>mummification</kwd><kwd>collagen</kwd><kwd>inflammation</kwd></kwd-group><funding-group specific-use="FundRef"><award-group><funding-source><institution-wrap><institution>Macquarie University Research Fellowship</institution></institution-wrap></funding-source></award-group></funding-group><custom-meta-group><custom-meta><meta-name>cover-date</meta-name><meta-value>October 28, 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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