Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mitochondrial function and increased convective O<sub>2</sub>
transport: implications for the assessment of mitochondrial respiration in vivo</title>
<author><name sortKey="Layec, Gwenael" sort="Layec, Gwenael" uniqKey="Layec G" first="Gwenael" last="Layec">Gwenael Layec</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Haseler, Luke J" sort="Haseler, Luke J" uniqKey="Haseler L" first="Luke J." last="Haseler">Luke J. Haseler</name>
<affiliation><nlm:aff id="aff3">Heart Foundation Research Centre, Griffith Health Institute, Griffith University, Queensland, Australia;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Trinity, Joel D" sort="Trinity, Joel D" uniqKey="Trinity J" first="Joel D." last="Trinity">Joel D. Trinity</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hart, Corey R" sort="Hart, Corey R" uniqKey="Hart C" first="Corey R." last="Hart">Corey R. Hart</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name>
<affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Le Fur, Yann" sort="Le Fur, Yann" uniqKey="Le Fur Y" first="Yann" last="Le Fur">Yann Le Fur</name>
<affiliation><nlm:aff id="aff6">Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jeong, Eun Kee" sort="Jeong, Eun Kee" uniqKey="Jeong E" first="Eun-Kee" last="Jeong">Eun-Kee Jeong</name>
<affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Richardson, Russell S" sort="Richardson, Russell S" uniqKey="Richardson R" first="Russell S." last="Richardson">Russell S. Richardson</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23813526</idno>
<idno type="pmc">3764626</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764626</idno>
<idno type="RBID">PMC:3764626</idno>
<idno type="doi">10.1152/japplphysiol.00257.2013</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">001907</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001907</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Mitochondrial function and increased convective O<sub>2</sub>
transport: implications for the assessment of mitochondrial respiration in vivo</title>
<author><name sortKey="Layec, Gwenael" sort="Layec, Gwenael" uniqKey="Layec G" first="Gwenael" last="Layec">Gwenael Layec</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Haseler, Luke J" sort="Haseler, Luke J" uniqKey="Haseler L" first="Luke J." last="Haseler">Luke J. Haseler</name>
<affiliation><nlm:aff id="aff3">Heart Foundation Research Centre, Griffith Health Institute, Griffith University, Queensland, Australia;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Trinity, Joel D" sort="Trinity, Joel D" uniqKey="Trinity J" first="Joel D." last="Trinity">Joel D. Trinity</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hart, Corey R" sort="Hart, Corey R" uniqKey="Hart C" first="Corey R." last="Hart">Corey R. Hart</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name>
<affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Le Fur, Yann" sort="Le Fur, Yann" uniqKey="Le Fur Y" first="Yann" last="Le Fur">Yann Le Fur</name>
<affiliation><nlm:aff id="aff6">Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jeong, Eun Kee" sort="Jeong, Eun Kee" uniqKey="Jeong E" first="Eun-Kee" last="Jeong">Eun-Kee Jeong</name>
<affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Richardson, Russell S" sort="Richardson, Russell S" uniqKey="Richardson R" first="Russell S." last="Richardson">Russell S. Richardson</name>
<affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Applied Physiology</title>
<idno type="ISSN">8750-7587</idno>
<idno type="eISSN">1522-1601</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Although phosphorus magnetic resonance spectroscopy (<sup>31</sup>
P-MRS)-based evidence suggests that in vivo peak mitochondrial respiration rate in young untrained adults is limited by the intrinsic mitochondrial capacity of ATP synthesis, it remains unknown whether a large, locally targeted increase in convective O<sub>2</sub>
delivery would alter this interpretation. Consequently, we examined the effect of superimposing reactive hyperemia (RH), induced by a period of brief ischemia during the last minute of exercise, on oxygen delivery and mitochondrial function in the calf muscle of nine young adults compared with free-flow conditions (FF). To this aim, we used an integrative experimental approach combining <sup>31</sup>
P-MRS, Doppler ultrasound imaging, and near-infrared spectroscopy. Limb blood flow [area under the curve (AUC), 1.4 ± 0.8 liters in FF and 2.5 ± 0.3 liters in RH, <italic>P</italic>
< 0.01] and convective O<sub>2</sub>
delivery (AUC, 0.30 ± 0.16 liters in FF and 0.54 ± 0.05 liters in RH, <italic>P</italic>
< 0.01), were significantly increased in RH compared with FF. RH was also associated with significantly higher capillary blood flow (<italic>P</italic>
< 0.05) and faster tissue reoxygenation mean response times (70 ± 15 s in FF and 24 ± 15 s in RH, <italic>P</italic>
< 0.05). This resulted in a 43% increase in estimated peak mitochondrial ATP synthesis rate (29 ± 13 mM/min in FF and 41 ± 14 mM/min in RH, <italic>P</italic>
< 0.05) whereas the phosphocreatine (PCr) recovery time constant in RH was not significantly different (<italic>P</italic>
= 0.22). This comprehensive assessment of local skeletal muscle O<sub>2</sub>
availability and utilization in untrained subjects reveals that mitochondrial function, assessed in vivo by <sup>31</sup>
P-MRS, is limited by convective O<sub>2</sub>
delivery rather than an intrinsic mitochondrial limitation.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Appl Physiol (1985)</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Appl. Physiol</journal-id>
<journal-id journal-id-type="hwp">jap</journal-id>
<journal-id journal-id-type="pmc">jap</journal-id>
<journal-id journal-id-type="publisher-id">JAPPLPHYSIOL</journal-id>
<journal-title-group><journal-title>Journal of Applied Physiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">8750-7587</issn>
<issn pub-type="epub">1522-1601</issn>
<publisher><publisher-name>American Physiological Society</publisher-name>
<publisher-loc>Bethesda, MD</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23813526</article-id>
<article-id pub-id-type="pmc">3764626</article-id>
<article-id pub-id-type="publisher-id">JAPPL-00257-2013</article-id>
<article-id pub-id-type="doi">10.1152/japplphysiol.00257.2013</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Mitochondrial function and increased convective O<sub>2</sub>
transport: implications for the assessment of mitochondrial respiration in vivo</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Layec</surname>
<given-names>Gwenael</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Haseler</surname>
<given-names>Luke J.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Trinity</surname>
<given-names>Joel D.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hart</surname>
<given-names>Corey R.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Xin</given-names>
</name>
<xref ref-type="aff" rid="aff5"><sup>5</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Le Fur</surname>
<given-names>Yann</given-names>
</name>
<xref ref-type="aff" rid="aff6"><sup>6</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jeong</surname>
<given-names>Eun-Kee</given-names>
</name>
<xref ref-type="aff" rid="aff5"><sup>5</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Richardson</surname>
<given-names>Russell S.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<aff id="aff1"><sup>1</sup>
Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</aff>
<aff id="aff2"><sup>2</sup>
Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</aff>
<aff id="aff3"><sup>3</sup>
Heart Foundation Research Centre, Griffith Health Institute, Griffith University, Queensland, Australia;</aff>
<aff id="aff4"><sup>4</sup>
Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</aff>
<aff id="aff5"><sup>5</sup>
Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah; and</aff>
<aff id="aff6"><sup>6</sup>
Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France</aff>
</contrib-group>
<author-notes><corresp id="cor1">Address for reprint requests and other correspondence: G. Layec, <addr-line>VA Medical Center, Bldg. 2, 500 Foothill Dr., Salt Lake City, UT 84148</addr-line>
(e-mail: <email>gwenael.layec@utah.edu</email>
).</corresp>
</author-notes>
<pub-date pub-type="epub"><day>27</day>
<month>6</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="ppub"><day>15</day>
<month>9</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>15</day>
<month>9</month>
<year>2014</year>
</pub-date>
<pmc-comment> PMC Release delay is 12 months and
0 days and was based on the . </pmc-comment>
<volume>115</volume>
<issue>6</issue>
<fpage>803</fpage>
<lpage>811</lpage>
<history><date date-type="received"><day>26</day>
<month>2</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>21</day>
<month>6</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2013 the American Physiological Society</copyright-statement>
<copyright-year>2013</copyright-year>
<copyright-holder>American Physiological Society</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zdg01813000803.pdf"></self-uri>
<abstract><p>Although phosphorus magnetic resonance spectroscopy (<sup>31</sup>
P-MRS)-based evidence suggests that in vivo peak mitochondrial respiration rate in young untrained adults is limited by the intrinsic mitochondrial capacity of ATP synthesis, it remains unknown whether a large, locally targeted increase in convective O<sub>2</sub>
delivery would alter this interpretation. Consequently, we examined the effect of superimposing reactive hyperemia (RH), induced by a period of brief ischemia during the last minute of exercise, on oxygen delivery and mitochondrial function in the calf muscle of nine young adults compared with free-flow conditions (FF). To this aim, we used an integrative experimental approach combining <sup>31</sup>
P-MRS, Doppler ultrasound imaging, and near-infrared spectroscopy. Limb blood flow [area under the curve (AUC), 1.4 ± 0.8 liters in FF and 2.5 ± 0.3 liters in RH, <italic>P</italic>
< 0.01] and convective O<sub>2</sub>
delivery (AUC, 0.30 ± 0.16 liters in FF and 0.54 ± 0.05 liters in RH, <italic>P</italic>
< 0.01), were significantly increased in RH compared with FF. RH was also associated with significantly higher capillary blood flow (<italic>P</italic>
< 0.05) and faster tissue reoxygenation mean response times (70 ± 15 s in FF and 24 ± 15 s in RH, <italic>P</italic>
< 0.05). This resulted in a 43% increase in estimated peak mitochondrial ATP synthesis rate (29 ± 13 mM/min in FF and 41 ± 14 mM/min in RH, <italic>P</italic>
< 0.05) whereas the phosphocreatine (PCr) recovery time constant in RH was not significantly different (<italic>P</italic>
= 0.22). This comprehensive assessment of local skeletal muscle O<sub>2</sub>
availability and utilization in untrained subjects reveals that mitochondrial function, assessed in vivo by <sup>31</sup>
P-MRS, is limited by convective O<sub>2</sub>
delivery rather than an intrinsic mitochondrial limitation.</p>
</abstract>
<kwd-group><kwd><sup>31</sup>
P-MRS</kwd>
<kwd>muscle energetics</kwd>
<kwd>PCr recovery kinetics</kwd>
<kwd>O<sub>2</sub>
delivery</kwd>
<kwd>mitochondrial function</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0019070 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0019070 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Pmc |étape= Corpus |type= RBID |clé= |texte= }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |