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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mitochondrial function and increased convective O<sub>2</sub> transport: implications for the assessment of mitochondrial respiration in vivo</title><author><name sortKey="Layec, Gwenael" sort="Layec, Gwenael" uniqKey="Layec G" first="Gwenael" last="Layec">Gwenael Layec</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation></author><author><name sortKey="Haseler, Luke J" sort="Haseler, Luke J" uniqKey="Haseler L" first="Luke J." last="Haseler">Luke J. Haseler</name><affiliation><nlm:aff id="aff3">Heart Foundation Research Centre, Griffith Health Institute, Griffith University, Queensland, Australia;</nlm:aff></affiliation></author><author><name sortKey="Trinity, Joel D" sort="Trinity, Joel D" uniqKey="Trinity J" first="Joel D." last="Trinity">Joel D. Trinity</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation></author><author><name sortKey="Hart, Corey R" sort="Hart, Corey R" uniqKey="Hart C" first="Corey R." last="Hart">Corey R. Hart</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation></author><author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name><affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff></affiliation></author><author><name sortKey="Le Fur, Yann" sort="Le Fur, Yann" uniqKey="Le Fur Y" first="Yann" last="Le Fur">Yann Le Fur</name><affiliation><nlm:aff id="aff6">Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Jeong, Eun Kee" sort="Jeong, Eun Kee" uniqKey="Jeong E" first="Eun-Kee" last="Jeong">Eun-Kee Jeong</name><affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff></affiliation></author><author><name sortKey="Richardson, Russell S" sort="Richardson, Russell S" uniqKey="Richardson R" first="Russell S." last="Richardson">Russell S. Richardson</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23813526</idno><idno type="pmc">3764626</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764626</idno><idno type="RBID">PMC:3764626</idno><idno type="doi">10.1152/japplphysiol.00257.2013</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001907</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001907</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Mitochondrial function and increased convective O<sub>2</sub> transport: implications for the assessment of mitochondrial respiration in vivo</title><author><name sortKey="Layec, Gwenael" sort="Layec, Gwenael" uniqKey="Layec G" first="Gwenael" last="Layec">Gwenael Layec</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation></author><author><name sortKey="Haseler, Luke J" sort="Haseler, Luke J" uniqKey="Haseler L" first="Luke J." last="Haseler">Luke J. Haseler</name><affiliation><nlm:aff id="aff3">Heart Foundation Research Centre, Griffith Health Institute, Griffith University, Queensland, Australia;</nlm:aff></affiliation></author><author><name sortKey="Trinity, Joel D" sort="Trinity, Joel D" uniqKey="Trinity J" first="Joel D." last="Trinity">Joel D. Trinity</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation></author><author><name sortKey="Hart, Corey R" sort="Hart, Corey R" uniqKey="Hart C" first="Corey R." last="Hart">Corey R. Hart</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation></author><author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name><affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff></affiliation></author><author><name sortKey="Le Fur, Yann" sort="Le Fur, Yann" uniqKey="Le Fur Y" first="Yann" last="Le Fur">Yann Le Fur</name><affiliation><nlm:aff id="aff6">Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Jeong, Eun Kee" sort="Jeong, Eun Kee" uniqKey="Jeong E" first="Eun-Kee" last="Jeong">Eun-Kee Jeong</name><affiliation><nlm:aff wicri:cut="; and" id="aff5">Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah</nlm:aff></affiliation></author><author><name sortKey="Richardson, Russell S" sort="Richardson, Russell S" uniqKey="Richardson R" first="Russell S." last="Richardson">Russell S. Richardson</name><affiliation><nlm:aff id="aff1">Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Applied Physiology</title><idno type="ISSN">8750-7587</idno><idno type="eISSN">1522-1601</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Although phosphorus magnetic resonance spectroscopy (<sup>31</sup>P-MRS)-based evidence suggests that in vivo peak mitochondrial respiration rate in young untrained adults is limited by the intrinsic mitochondrial capacity of ATP synthesis, it remains unknown whether a large, locally targeted increase in convective O<sub>2</sub> delivery would alter this interpretation. Consequently, we examined the effect of superimposing reactive hyperemia (RH), induced by a period of brief ischemia during the last minute of exercise, on oxygen delivery and mitochondrial function in the calf muscle of nine young adults compared with free-flow conditions (FF). To this aim, we used an integrative experimental approach combining <sup>31</sup>P-MRS, Doppler ultrasound imaging, and near-infrared spectroscopy. Limb blood flow [area under the curve (AUC), 1.4 ± 0.8 liters in FF and 2.5 ± 0.3 liters in RH, <italic>P</italic> < 0.01] and convective O<sub>2</sub> delivery (AUC, 0.30 ± 0.16 liters in FF and 0.54 ± 0.05 liters in RH, <italic>P</italic> < 0.01), were significantly increased in RH compared with FF. RH was also associated with significantly higher capillary blood flow (<italic>P</italic> < 0.05) and faster tissue reoxygenation mean response times (70 ± 15 s in FF and 24 ± 15 s in RH, <italic>P</italic> < 0.05). This resulted in a 43% increase in estimated peak mitochondrial ATP synthesis rate (29 ± 13 mM/min in FF and 41 ± 14 mM/min in RH, <italic>P</italic> < 0.05) whereas the phosphocreatine (PCr) recovery time constant in RH was not significantly different (<italic>P</italic> = 0.22). This comprehensive assessment of local skeletal muscle O<sub>2</sub> availability and utilization in untrained subjects reveals that mitochondrial function, assessed in vivo by <sup>31</sup>P-MRS, is limited by convective O<sub>2</sub> delivery rather than an intrinsic mitochondrial limitation.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Appl Physiol (1985)</journal-id><journal-id journal-id-type="iso-abbrev">J. Appl. Physiol</journal-id><journal-id journal-id-type="hwp">jap</journal-id><journal-id journal-id-type="pmc">jap</journal-id><journal-id journal-id-type="publisher-id">JAPPLPHYSIOL</journal-id><journal-title-group><journal-title>Journal of Applied Physiology</journal-title></journal-title-group><issn pub-type="ppub">8750-7587</issn><issn pub-type="epub">1522-1601</issn><publisher><publisher-name>American Physiological Society</publisher-name><publisher-loc>Bethesda, MD</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23813526</article-id><article-id pub-id-type="pmc">3764626</article-id><article-id pub-id-type="publisher-id">JAPPL-00257-2013</article-id><article-id pub-id-type="doi">10.1152/japplphysiol.00257.2013</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Mitochondrial function and increased convective O<sub>2</sub> transport: implications for the assessment of mitochondrial respiration in vivo</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Layec</surname><given-names>Gwenael</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Haseler</surname><given-names>Luke J.</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Trinity</surname><given-names>Joel D.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hart</surname><given-names>Corey R.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Xin</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Le Fur</surname><given-names>Yann</given-names></name><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jeong</surname><given-names>Eun-Kee</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Richardson</surname><given-names>Russell S.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><aff id="aff1"><sup>1</sup>Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, Utah;</aff><aff id="aff2"><sup>2</sup>Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah;</aff><aff id="aff3"><sup>3</sup>Heart Foundation Research Centre, Griffith Health Institute, Griffith University, Queensland, Australia;</aff><aff id="aff4"><sup>4</sup>Exercise and Sport Science, University of Utah, Salt Lake City, Utah;</aff><aff id="aff5"><sup>5</sup>Department of Radiology and Utah Center for Advanced Imaging Research, University of Utah, Salt Lake City, Utah; and</aff><aff id="aff6"><sup>6</sup>Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France</aff></contrib-group><author-notes><corresp id="cor1">Address for reprint requests and other correspondence: G. Layec, <addr-line>VA Medical Center, Bldg. 2, 500 Foothill Dr., Salt Lake City, UT 84148</addr-line> (e-mail: <email>gwenael.layec@utah.edu</email>).</corresp></author-notes><pub-date pub-type="epub"><day>27</day><month>6</month><year>2013</year></pub-date><pub-date pub-type="ppub"><day>15</day><month>9</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>15</day><month>9</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 12 months and
0 days and was based on the
<volume>115</volume><issue>6</issue><fpage>803</fpage><lpage>811</lpage><history><date date-type="received"><day>26</day><month>2</month><year>2013</year></date><date date-type="accepted"><day>21</day><month>6</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013 the American Physiological Society</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>American Physiological Society</copyright-holder></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zdg01813000803.pdf"></self-uri><abstract><p>Although phosphorus magnetic resonance spectroscopy (<sup>31</sup>P-MRS)-based evidence suggests that in vivo peak mitochondrial respiration rate in young untrained adults is limited by the intrinsic mitochondrial capacity of ATP synthesis, it remains unknown whether a large, locally targeted increase in convective O<sub>2</sub> delivery would alter this interpretation. Consequently, we examined the effect of superimposing reactive hyperemia (RH), induced by a period of brief ischemia during the last minute of exercise, on oxygen delivery and mitochondrial function in the calf muscle of nine young adults compared with free-flow conditions (FF). To this aim, we used an integrative experimental approach combining <sup>31</sup>P-MRS, Doppler ultrasound imaging, and near-infrared spectroscopy. Limb blood flow [area under the curve (AUC), 1.4 ± 0.8 liters in FF and 2.5 ± 0.3 liters in RH, <italic>P</italic> < 0.01] and convective O<sub>2</sub> delivery (AUC, 0.30 ± 0.16 liters in FF and 0.54 ± 0.05 liters in RH, <italic>P</italic> < 0.01), were significantly increased in RH compared with FF. RH was also associated with significantly higher capillary blood flow (<italic>P</italic> < 0.05) and faster tissue reoxygenation mean response times (70 ± 15 s in FF and 24 ± 15 s in RH, <italic>P</italic> < 0.05). This resulted in a 43% increase in estimated peak mitochondrial ATP synthesis rate (29 ± 13 mM/min in FF and 41 ± 14 mM/min in RH, <italic>P</italic> < 0.05) whereas the phosphocreatine (PCr) recovery time constant in RH was not significantly different (<italic>P</italic> = 0.22). This comprehensive assessment of local skeletal muscle O<sub>2</sub> availability and utilization in untrained subjects reveals that mitochondrial function, assessed in vivo by <sup>31</sup>P-MRS, is limited by convective O<sub>2</sub> delivery rather than an intrinsic mitochondrial limitation.</p></abstract><kwd-group><kwd><sup>31</sup>P-MRS</kwd><kwd>muscle energetics</kwd><kwd>PCr recovery kinetics</kwd><kwd>O<sub>2</sub> delivery</kwd><kwd>mitochondrial function</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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