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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><named-content content-type="genus-species">Leptospira interrogans</named-content> Catalase Is Required for Resistance to H<sub>2</sub>O<sub>2</sub> and for Virulence</title><author><name sortKey="Eshghi, Azad" sort="Eshghi, Azad" uniqKey="Eshghi A" first="Azad" last="Eshghi">Azad Eshghi</name><affiliation><nlm:aff id="aff1">Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</nlm:aff></affiliation></author><author><name sortKey="Lourdault, Kristel" sort="Lourdault, Kristel" uniqKey="Lourdault K" first="Kristel" last="Lourdault">Kristel Lourdault</name><affiliation><nlm:aff id="aff2">Institut Pasteur, Unité de Biologie des Spirochétes, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Murray, Gerald L" sort="Murray, Gerald L" uniqKey="Murray G" first="Gerald L." last="Murray">Gerald L. Murray</name><affiliation><nlm:aff id="aff3">Department of Microbiology, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation></author><author><name sortKey="Bartpho, Thanatchaporn" sort="Bartpho, Thanatchaporn" uniqKey="Bartpho T" first="Thanatchaporn" last="Bartpho">Thanatchaporn Bartpho</name><affiliation><nlm:aff id="aff4">Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand</nlm:aff></affiliation></author><author><name sortKey="Sermswan, Rasana W" sort="Sermswan, Rasana W" uniqKey="Sermswan R" first="Rasana W." last="Sermswan">Rasana W. Sermswan</name><affiliation><nlm:aff id="aff5">Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand</nlm:aff></affiliation></author><author><name sortKey="Picardeau, Mathieu" sort="Picardeau, Mathieu" uniqKey="Picardeau M" first="Mathieu" last="Picardeau">Mathieu Picardeau</name><affiliation><nlm:aff id="aff2">Institut Pasteur, Unité de Biologie des Spirochétes, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Adler, Ben" sort="Adler, Ben" uniqKey="Adler B" first="Ben" last="Adler">Ben Adler</name><affiliation><nlm:aff id="aff3">Department of Microbiology, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation></author><author><name sortKey="Snarr, Brendan" sort="Snarr, Brendan" uniqKey="Snarr B" first="Brendan" last="Snarr">Brendan Snarr</name><affiliation><nlm:aff id="aff1">Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</nlm:aff></affiliation></author><author><name sortKey="Zuerner, Richard L" sort="Zuerner, Richard L" uniqKey="Zuerner R" first="Richard L." last="Zuerner">Richard L. Zuerner</name><affiliation><nlm:aff id="aff7">Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Uppsala, Sweden</nlm:aff></affiliation></author><author><name sortKey="Cameron, Caroline E" sort="Cameron, Caroline E" uniqKey="Cameron C" first="Caroline E." last="Cameron">Caroline E. Cameron</name><affiliation><nlm:aff id="aff1">Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22927050</idno><idno type="pmc">3486042</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486042</idno><idno type="RBID">PMC:3486042</idno><idno type="doi">10.1128/IAI.00466-12</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">001891</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001891</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><named-content content-type="genus-species">Leptospira interrogans</named-content> Catalase Is Required for Resistance to H<sub>2</sub>O<sub>2</sub> and for Virulence</title><author><name sortKey="Eshghi, Azad" sort="Eshghi, Azad" uniqKey="Eshghi A" first="Azad" last="Eshghi">Azad Eshghi</name><affiliation><nlm:aff id="aff1">Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</nlm:aff></affiliation></author><author><name sortKey="Lourdault, Kristel" sort="Lourdault, Kristel" uniqKey="Lourdault K" first="Kristel" last="Lourdault">Kristel Lourdault</name><affiliation><nlm:aff id="aff2">Institut Pasteur, Unité de Biologie des Spirochétes, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Murray, Gerald L" sort="Murray, Gerald L" uniqKey="Murray G" first="Gerald L." last="Murray">Gerald L. Murray</name><affiliation><nlm:aff id="aff3">Department of Microbiology, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation></author><author><name sortKey="Bartpho, Thanatchaporn" sort="Bartpho, Thanatchaporn" uniqKey="Bartpho T" first="Thanatchaporn" last="Bartpho">Thanatchaporn Bartpho</name><affiliation><nlm:aff id="aff4">Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand</nlm:aff></affiliation></author><author><name sortKey="Sermswan, Rasana W" sort="Sermswan, Rasana W" uniqKey="Sermswan R" first="Rasana W." last="Sermswan">Rasana W. Sermswan</name><affiliation><nlm:aff id="aff5">Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand</nlm:aff></affiliation></author><author><name sortKey="Picardeau, Mathieu" sort="Picardeau, Mathieu" uniqKey="Picardeau M" first="Mathieu" last="Picardeau">Mathieu Picardeau</name><affiliation><nlm:aff id="aff2">Institut Pasteur, Unité de Biologie des Spirochétes, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Adler, Ben" sort="Adler, Ben" uniqKey="Adler B" first="Ben" last="Adler">Ben Adler</name><affiliation><nlm:aff id="aff3">Department of Microbiology, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria, Australia</nlm:aff></affiliation></author><author><name sortKey="Snarr, Brendan" sort="Snarr, Brendan" uniqKey="Snarr B" first="Brendan" last="Snarr">Brendan Snarr</name><affiliation><nlm:aff id="aff1">Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</nlm:aff></affiliation></author><author><name sortKey="Zuerner, Richard L" sort="Zuerner, Richard L" uniqKey="Zuerner R" first="Richard L." last="Zuerner">Richard L. Zuerner</name><affiliation><nlm:aff id="aff7">Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Uppsala, Sweden</nlm:aff></affiliation></author><author><name sortKey="Cameron, Caroline E" sort="Cameron, Caroline E" uniqKey="Cameron C" first="Caroline E." last="Cameron">Caroline E. Cameron</name><affiliation><nlm:aff id="aff1">Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</nlm:aff></affiliation></author></analytic><series><title level="j">Infection and Immunity</title><idno type="ISSN">0019-9567</idno><idno type="eISSN">1098-5522</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Pathogenic <named-content content-type="genus-species">Leptospira</named-content> spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized <named-content content-type="genus-species">Leptospira interrogans</named-content> catalase (KatE), the only annotated catalase found within pathogenic <named-content content-type="genus-species">Leptospira</named-content> species, by assessing its role in resistance to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and during infection in hamsters. Pathogenic <named-content content-type="genus-species">L. interrogans</named-content> bacteria had a 50-fold-higher survival rate under H<sub>2</sub>O<sub>2</sub>-induced oxidative stress than did saprophytic <named-content content-type="genus-species">L. biflexa</named-content> bacteria, and this was predominantly catalase dependent. We also characterized KatE, the only annotated catalase found within pathogenic <named-content content-type="genus-species">Leptospira</named-content> species. Catalase assays performed with recombinant KatE confirmed specific catalase activity, while protein fractionation experiments localized KatE to the bacterial periplasmic space. The insertional inactivation of <italic>katE</italic> in pathogenic <named-content content-type="genus-species">Leptospira</named-content> bacteria drastically diminished leptospiral viability in the presence of extracellular H<sub>2</sub>O<sub>2</sub> and reduced virulence in an acute-infection model. Combined, these results suggest that <named-content content-type="genus-species">L. interrogans</named-content> KatE confers <italic>in vivo</italic> resistance to reactive oxygen species induced by the host innate immune response.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Immun</journal-id><journal-id journal-id-type="iso-abbrev">Infect. Immun</journal-id><journal-id journal-id-type="hwp">iai</journal-id><journal-id journal-id-type="pmc">iai</journal-id><journal-id journal-id-type="publisher-id">IAI</journal-id><journal-title-group><journal-title>Infection and Immunity</journal-title></journal-title-group><issn pub-type="ppub">0019-9567</issn><issn pub-type="epub">1098-5522</issn><publisher><publisher-name>American Society for Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22927050</article-id><article-id pub-id-type="pmc">3486042</article-id><article-id pub-id-type="publisher-id">00466-12</article-id><article-id pub-id-type="doi">10.1128/IAI.00466-12</article-id><article-categories><subj-group subj-group-type="heading"><subject>Molecular Pathogenesis</subject></subj-group></article-categories><title-group><article-title><named-content content-type="genus-species">Leptospira interrogans</named-content> Catalase Is Required for Resistance to H<sub>2</sub>O<sub>2</sub> and for Virulence</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Eshghi</surname><given-names>Azad</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lourdault</surname><given-names>Kristel</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Murray</surname><given-names>Gerald L.</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bartpho</surname><given-names>Thanatchaporn</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sermswan</surname><given-names>Rasana W.</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><contrib contrib-type="author"><name><surname>Picardeau</surname><given-names>Mathieu</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Adler</surname><given-names>Ben</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Snarr</surname><given-names>Brendan</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zuerner</surname><given-names>Richard L.</given-names></name><xref ref-type="aff" rid="aff7"><sup>g</sup></xref><xref ref-type="aff" rid="aff8"><sup>h</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Cameron</surname><given-names>Caroline E.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><aff id="aff1"><label>a</label>Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada</aff><aff id="aff2"><label>b</label>Institut Pasteur, Unité de Biologie des Spirochétes, Paris, France</aff><aff id="aff3"><label>c</label>Department of Microbiology, Monash University, Clayton, Victoria, Australia</aff><aff id="aff4"><label>d</label>Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand</aff><aff id="aff5"><label>e</label>Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand</aff><aff id="aff6"><label>f</label>Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria, Australia</aff><aff id="aff7"><label>g</label>Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA</aff><aff id="aff8"><label>h</label>Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Uppsala, Sweden</aff></contrib-group><contrib-group><contrib contrib-type="editor"><name><surname>Blanke</surname><given-names>S. R.</given-names></name><role>Editor</role></contrib></contrib-group><author-notes><corresp>Address correspondence to Caroline E. Cameron, <email>caroc@uvic.ca</email>.</corresp></author-notes><pub-date pub-type="ppub"><month>11</month><year>2012</year></pub-date><volume>80</volume><issue>11</issue><fpage>3892</fpage><lpage>3899</lpage><history><date date-type="received"><day>4</day><month>5</month><year>2012</year></date><date date-type="rev-request"><day>30</day><month>5</month><year>2012</year></date><date date-type="accepted"><day>16</day><month>8</month><year>2012</year></date></history><permissions><copyright-statement>Copyright © 2012, American Society for Microbiology. All Rights Reserved.</copyright-statement><copyright-year>2012</copyright-year><copyright-holder>American Society for Microbiology</copyright-holder></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zii01112003892.pdf"></self-uri><abstract><p>Pathogenic <named-content content-type="genus-species">Leptospira</named-content> spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized <named-content content-type="genus-species">Leptospira interrogans</named-content> catalase (KatE), the only annotated catalase found within pathogenic <named-content content-type="genus-species">Leptospira</named-content> species, by assessing its role in resistance to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and during infection in hamsters. Pathogenic <named-content content-type="genus-species">L. interrogans</named-content> bacteria had a 50-fold-higher survival rate under H<sub>2</sub>O<sub>2</sub>-induced oxidative stress than did saprophytic <named-content content-type="genus-species">L. biflexa</named-content> bacteria, and this was predominantly catalase dependent. We also characterized KatE, the only annotated catalase found within pathogenic <named-content content-type="genus-species">Leptospira</named-content> species. Catalase assays performed with recombinant KatE confirmed specific catalase activity, while protein fractionation experiments localized KatE to the bacterial periplasmic space. The insertional inactivation of <italic>katE</italic> in pathogenic <named-content content-type="genus-species">Leptospira</named-content> bacteria drastically diminished leptospiral viability in the presence of extracellular H<sub>2</sub>O<sub>2</sub> and reduced virulence in an acute-infection model. Combined, these results suggest that <named-content content-type="genus-species">L. interrogans</named-content> KatE confers <italic>in vivo</italic> resistance to reactive oxygen species induced by the host innate immune response.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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