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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice</title><author><name sortKey="Wilhelm, Dagmar" sort="Wilhelm, Dagmar" uniqKey="Wilhelm D" first="Dagmar" last="Wilhelm">Dagmar Wilhelm</name><affiliation><nlm:aff id="A1"> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Washburn, Linda L" sort="Washburn, Linda L" uniqKey="Washburn L" first="Linda L." last="Washburn">Linda L. Washburn</name><affiliation><nlm:aff id="A2"> The Jackson Laboratory, Bar Harbor, Maine, USA</nlm:aff></affiliation></author><author><name sortKey="Truong, Vy" sort="Truong, Vy" uniqKey="Truong V" first="Vy" last="Truong">Vy Truong</name><affiliation><nlm:aff id="A1"> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Fellous, Marc" sort="Fellous, Marc" uniqKey="Fellous M" first="Marc" last="Fellous">Marc Fellous</name><affiliation><nlm:aff id="A3"> Cochin Institute, 75014 Paris, France</nlm:aff></affiliation></author><author><name sortKey="Eicher, Eva M" sort="Eicher, Eva M" uniqKey="Eicher E" first="Eva M." last="Eicher">Eva M. Eicher</name><affiliation><nlm:aff id="A2"> The Jackson Laboratory, Bar Harbor, Maine, USA</nlm:aff></affiliation></author><author><name sortKey="Koopman, Peter" sort="Koopman, Peter" uniqKey="Koopman P" first="Peter" last="Koopman">Peter Koopman</name><affiliation><nlm:aff id="A1"> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="A4"> ARC Centre of Excellence in Biotechnology and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19269320</idno><idno type="pmc">2680453</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680453</idno><idno type="RBID">PMC:2680453</idno><idno type="doi">10.1016/j.mod.2009.02.006</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">001470</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001470</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice</title><author><name sortKey="Wilhelm, Dagmar" sort="Wilhelm, Dagmar" uniqKey="Wilhelm D" first="Dagmar" last="Wilhelm">Dagmar Wilhelm</name><affiliation><nlm:aff id="A1"> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Washburn, Linda L" sort="Washburn, Linda L" uniqKey="Washburn L" first="Linda L." last="Washburn">Linda L. Washburn</name><affiliation><nlm:aff id="A2"> The Jackson Laboratory, Bar Harbor, Maine, USA</nlm:aff></affiliation></author><author><name sortKey="Truong, Vy" sort="Truong, Vy" uniqKey="Truong V" first="Vy" last="Truong">Vy Truong</name><affiliation><nlm:aff id="A1"> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation></author><author><name sortKey="Fellous, Marc" sort="Fellous, Marc" uniqKey="Fellous M" first="Marc" last="Fellous">Marc Fellous</name><affiliation><nlm:aff id="A3"> Cochin Institute, 75014 Paris, France</nlm:aff></affiliation></author><author><name sortKey="Eicher, Eva M" sort="Eicher, Eva M" uniqKey="Eicher E" first="Eva M." last="Eicher">Eva M. Eicher</name><affiliation><nlm:aff id="A2"> The Jackson Laboratory, Bar Harbor, Maine, USA</nlm:aff></affiliation></author><author><name sortKey="Koopman, Peter" sort="Koopman, Peter" uniqKey="Koopman P" first="Peter" last="Koopman">Peter Koopman</name><affiliation><nlm:aff id="A1"> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="A4"> ARC Centre of Excellence in Biotechnology and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</nlm:aff></affiliation></author></analytic><series><title level="j">Mechanisms of development</title><idno type="ISSN">0925-4773</idno><idno type="eISSN">1872-6356</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">Ovotestis development in B6-XY<sup>POS</sup> mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, <italic>Rspo1</italic>) development in B6-XY<sup>POS</sup> ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not upregulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate <italic>Sox9</italic>; (2) challenge the established view that SOX9 is responsible for down-regulating <italic>Sry</italic> expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9101218</journal-id><journal-id journal-id-type="pubmed-jr-id">1825</journal-id><journal-id journal-id-type="nlm-ta">Mech Dev</journal-id><journal-title>Mechanisms of development</journal-title><issn pub-type="ppub">0925-4773</issn><issn pub-type="epub">1872-6356</issn></journal-meta><article-meta><article-id pub-id-type="pmid">19269320</article-id><article-id pub-id-type="pmc">2680453</article-id><article-id pub-id-type="doi">10.1016/j.mod.2009.02.006</article-id><article-id pub-id-type="manuscript">NIHMS100359</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wilhelm</surname><given-names>Dagmar</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Washburn</surname><given-names>Linda L.</given-names></name><xref rid="A2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Truong</surname><given-names>Vy</given-names></name><xref rid="A1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Fellous</surname><given-names>Marc</given-names></name><xref rid="A3" ref-type="aff">3</xref></contrib><contrib contrib-type="author"><name><surname>Eicher</surname><given-names>Eva M.</given-names></name><xref rid="A2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Koopman</surname><given-names>Peter</given-names></name><xref rid="A1" ref-type="aff">1</xref><xref rid="A4" ref-type="aff">4</xref><xref rid="FN1" ref-type="author-notes">5</xref></contrib></contrib-group><aff id="A1"><label>1</label> Division of Molecular Genetics and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</aff><aff id="A2"><label>2</label> The Jackson Laboratory, Bar Harbor, Maine, USA</aff><aff id="A3"><label>3</label> Cochin Institute, 75014 Paris, France</aff><aff id="A4"><label>4</label> ARC Centre of Excellence in Biotechnology and Development, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia</aff><author-notes><corresp id="FN1"><label>5</label>Author for correspondence: Tel: +61 7 3346 2059, Fax: +61 7 3346 2101, Email: <email>p.koopman@imb.uq.edu.au</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>23</day><month>3</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>6</day><month>3</month><year>2009</year></pub-date><pub-date pub-type="ppub"><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>5</month><year>2010</year></pub-date><volume>126</volume><issue>5-6</issue><fpage>324</fpage><lpage>336</lpage><abstract><p id="P2">Ovotestis development in B6-XY<sup>POS</sup> mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, <italic>Rspo1</italic>) development in B6-XY<sup>POS</sup> ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not upregulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate <italic>Sox9</italic>; (2) challenge the established view that SOX9 is responsible for down-regulating <italic>Sry</italic> expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development.</p></abstract><kwd-group><kwd>Sry</kwd><kwd>Sox9</kwd><kwd>FoxL2</kwd><kwd>sex determination</kwd><kwd>gonad differentiation</kwd></kwd-group><contract-num rid="HD1">R03 HD049431-02</contract-num><contract-num rid="GM1">R01 GM020919-32</contract-num><contract-sponsor id="HD1">National Institute of Child Health & Human Development : NICHD</contract-sponsor><contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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