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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alternative splicing within the I–II loop controls surface expression of T-type Ca<sub>v</sub>3.1 calcium channels</title><author><name sortKey="Shcheglovitov, Aleksandr" sort="Shcheglovitov, Aleksandr" uniqKey="Shcheglovitov A" first="Aleksandr" last="Shcheglovitov">Aleksandr Shcheglovitov</name><affiliation><nlm:aff id="A1">Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author><author><name sortKey="Vitko, Iuliia" sort="Vitko, Iuliia" uniqKey="Vitko I" first="Iuliia" last="Vitko">Iuliia Vitko</name><affiliation><nlm:aff id="A1">Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author><author><name sortKey="Bidaud, Isabelle" sort="Bidaud, Isabelle" uniqKey="Bidaud I" first="Isabelle" last="Bidaud">Isabelle Bidaud</name><affiliation><nlm:aff id="A2">CNRS, UMR 5203, Institut de Génomique fonctionnelle, Montpellier, France</nlm:aff></affiliation><affiliation><nlm:aff id="A3">INSERM, U661, Montpellier, France.</nlm:aff></affiliation><affiliation><nlm:aff id="A4">Université Montpellier, 1 et 2, Montpellier, France.</nlm:aff></affiliation></author><author><name sortKey="Baumgart, Joel P" sort="Baumgart, Joel P" uniqKey="Baumgart J" first="Joel P." last="Baumgart">Joel P. Baumgart</name><affiliation><nlm:aff id="A5">Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author><author><name sortKey="Navarro Gonzalez, Manuel F" sort="Navarro Gonzalez, Manuel F" uniqKey="Navarro Gonzalez M" first="Manuel F." last="Navarro-Gonzalez">Manuel F. Navarro-Gonzalez</name></author><author><name sortKey="Grayson, T Hilton" sort="Grayson, T Hilton" uniqKey="Grayson T" first="T. Hilton" last="Grayson">T. Hilton Grayson</name></author><author><name sortKey="Lory, Philippe" sort="Lory, Philippe" uniqKey="Lory P" first="Philippe" last="Lory">Philippe Lory</name><affiliation><nlm:aff id="A2">CNRS, UMR 5203, Institut de Génomique fonctionnelle, Montpellier, France</nlm:aff></affiliation><affiliation><nlm:aff id="A3">INSERM, U661, Montpellier, France.</nlm:aff></affiliation><affiliation><nlm:aff id="A4">Université Montpellier, 1 et 2, Montpellier, France.</nlm:aff></affiliation></author><author><name sortKey="Hill, Caryl E" sort="Hill, Caryl E" uniqKey="Hill C" first="Caryl E." last="Hill">Caryl E. Hill</name><affiliation><nlm:aff id="A6">Division of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia.</nlm:aff></affiliation></author><author><name sortKey="Perez Reyes, Edward" sort="Perez Reyes, Edward" uniqKey="Perez Reyes E" first="Edward" last="Perez-Reyes">Edward Perez-Reyes</name><affiliation><nlm:aff id="A1">Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation><affiliation><nlm:aff id="A5">Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18930057</idno><idno type="pmc">2614674</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614674</idno><idno type="RBID">PMC:2614674</idno><idno type="doi">10.1016/j.febslet.2008.10.013</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">001396</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001396</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Alternative splicing within the I–II loop controls surface expression of T-type Ca<sub>v</sub>3.1 calcium channels</title><author><name sortKey="Shcheglovitov, Aleksandr" sort="Shcheglovitov, Aleksandr" uniqKey="Shcheglovitov A" first="Aleksandr" last="Shcheglovitov">Aleksandr Shcheglovitov</name><affiliation><nlm:aff id="A1">Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author><author><name sortKey="Vitko, Iuliia" sort="Vitko, Iuliia" uniqKey="Vitko I" first="Iuliia" last="Vitko">Iuliia Vitko</name><affiliation><nlm:aff id="A1">Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author><author><name sortKey="Bidaud, Isabelle" sort="Bidaud, Isabelle" uniqKey="Bidaud I" first="Isabelle" last="Bidaud">Isabelle Bidaud</name><affiliation><nlm:aff id="A2">CNRS, UMR 5203, Institut de Génomique fonctionnelle, Montpellier, France</nlm:aff></affiliation><affiliation><nlm:aff id="A3">INSERM, U661, Montpellier, France.</nlm:aff></affiliation><affiliation><nlm:aff id="A4">Université Montpellier, 1 et 2, Montpellier, France.</nlm:aff></affiliation></author><author><name sortKey="Baumgart, Joel P" sort="Baumgart, Joel P" uniqKey="Baumgart J" first="Joel P." last="Baumgart">Joel P. Baumgart</name><affiliation><nlm:aff id="A5">Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author><author><name sortKey="Navarro Gonzalez, Manuel F" sort="Navarro Gonzalez, Manuel F" uniqKey="Navarro Gonzalez M" first="Manuel F." last="Navarro-Gonzalez">Manuel F. Navarro-Gonzalez</name></author><author><name sortKey="Grayson, T Hilton" sort="Grayson, T Hilton" uniqKey="Grayson T" first="T. Hilton" last="Grayson">T. Hilton Grayson</name></author><author><name sortKey="Lory, Philippe" sort="Lory, Philippe" uniqKey="Lory P" first="Philippe" last="Lory">Philippe Lory</name><affiliation><nlm:aff id="A2">CNRS, UMR 5203, Institut de Génomique fonctionnelle, Montpellier, France</nlm:aff></affiliation><affiliation><nlm:aff id="A3">INSERM, U661, Montpellier, France.</nlm:aff></affiliation><affiliation><nlm:aff id="A4">Université Montpellier, 1 et 2, Montpellier, France.</nlm:aff></affiliation></author><author><name sortKey="Hill, Caryl E" sort="Hill, Caryl E" uniqKey="Hill C" first="Caryl E." last="Hill">Caryl E. Hill</name><affiliation><nlm:aff id="A6">Division of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia.</nlm:aff></affiliation></author><author><name sortKey="Perez Reyes, Edward" sort="Perez Reyes, Edward" uniqKey="Perez Reyes E" first="Edward" last="Perez-Reyes">Edward Perez-Reyes</name><affiliation><nlm:aff id="A1">Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation><affiliation><nlm:aff id="A5">Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</nlm:aff></affiliation></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">Molecular diversity of T-type/Ca<sub>v</sub>3 Ca<sup>2+</sup> channels is created by expression of three genes and alternative splicing of those genes. Prompted by the important role of the I–II linker in gating and surface expression of Ca<sub>v</sub>3 channels, we describe here the properties of a novel variant that partially deletes this loop. The variant is abundantly expressed in rat brain, even exceeding transcripts with the complete exon 8. Electrophysiological analysis of the Δ8b variant revealed enhanced current density compared to Ca<sub>v</sub>3.1a, but similar gating. Luminometry experiments revealed an increase in the expression of Δ8b channels at the plasma membrane. We conclude that alternative splicing of Ca<sub>v</sub>3 channels regulates surface expression and may underlie disease states in which T-channel current density is increased.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0155157</journal-id><journal-id journal-id-type="pubmed-jr-id">3696</journal-id><journal-id journal-id-type="nlm-ta">FEBS Lett</journal-id><journal-title>FEBS letters</journal-title><issn pub-type="ppub">0014-5793</issn></journal-meta><article-meta><article-id pub-id-type="pmid">18930057</article-id><article-id pub-id-type="pmc">2614674</article-id><article-id pub-id-type="manuscript">NIHMS79386</article-id><article-id pub-id-type="doi">10.1016/j.febslet.2008.10.013</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Alternative splicing within the I–II loop controls surface expression of T-type Ca<sub>v</sub>3.1 calcium channels</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Shcheglovitov</surname><given-names>Aleksandr</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib><contrib contrib-type="author"><name><surname>Vitko</surname><given-names>Iuliia</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib><contrib contrib-type="author"><name><surname>Bidaud</surname><given-names>Isabelle</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A3">3</xref><xref ref-type="aff" rid="A4">4</xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib><contrib contrib-type="author"><name><surname>Baumgart</surname><given-names>Joel P.</given-names></name><xref ref-type="aff" rid="A5">5</xref></contrib><contrib contrib-type="author"><name><surname>Navarro-Gonzalez</surname><given-names>Manuel F.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Grayson</surname><given-names>T. Hilton</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lory</surname><given-names>Philippe</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="aff" rid="A3">3</xref><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Hill</surname><given-names>Caryl E.</given-names></name><xref ref-type="aff" rid="A6">6</xref></contrib><contrib contrib-type="author"><name><surname>Perez-Reyes</surname><given-names>Edward</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A5">5</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</aff><aff id="A2"><label>2</label>CNRS, UMR 5203, Institut de Génomique fonctionnelle, Montpellier, France</aff><aff id="A3"><label>3</label>INSERM, U661, Montpellier, France.</aff><aff id="A4"><label>4</label>Université Montpellier, 1 et 2, Montpellier, France.</aff><aff id="A5"><label>5</label>Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia 22908, U.S.A.</aff><aff id="A6"><label>6</label>Division of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia.</aff><author-notes><fn id="FN1" fn-type="equal"><label>*</label><p id="P1">A.S., I.V., and I.B. contributed equally to this work</p></fn><corresp id="cor1">Address correspondence to either: Edward Perez-Reyes, Department of Pharmacology, University of Virginia, Charlottesville, VA 29908, U.S.A., E-mail: <email>eperez@virginia.edu</email>; or Caryl Hill, Division of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia, E-mail: <email>Caryl.Hill@anu.edu.au</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>19</day><month>11</month><year>2008</year></pub-date><pub-date pub-type="epub"><day>16</day><month>10</month><year>2008</year></pub-date><pub-date pub-type="ppub"><day>12</day><month>11</month><year>2008</year></pub-date><pub-date pub-type="pmc-release"><day>12</day><month>11</month><year>2009</year></pub-date><volume>582</volume><issue>27</issue><fpage>3765</fpage><lpage>3770</lpage><abstract><p id="P2">Molecular diversity of T-type/Ca<sub>v</sub>3 Ca<sup>2+</sup> channels is created by expression of three genes and alternative splicing of those genes. Prompted by the important role of the I–II linker in gating and surface expression of Ca<sub>v</sub>3 channels, we describe here the properties of a novel variant that partially deletes this loop. The variant is abundantly expressed in rat brain, even exceeding transcripts with the complete exon 8. Electrophysiological analysis of the Δ8b variant revealed enhanced current density compared to Ca<sub>v</sub>3.1a, but similar gating. Luminometry experiments revealed an increase in the expression of Δ8b channels at the plasma membrane. We conclude that alternative splicing of Ca<sub>v</sub>3 channels regulates surface expression and may underlie disease states in which T-channel current density is increased.</p></abstract><contract-num rid="NS1">R01 NS038691-08</contract-num><contract-sponsor id="NS1">National Institute of Neurological Disorders and Stroke : NINDS</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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