Bartonella australis sp. nov. from Kangaroos, Australia
Identifieur interne : 001316 ( Pmc/Corpus ); précédent : 001315; suivant : 001317Bartonella australis sp. nov. from Kangaroos, Australia
Auteurs : Pierre-Edouard Fournier ; Carmel Taylor ; Jean-Marc Rolain ; Lina Barrassi ; Greg Smith ; Didier RaoultSource :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2007.
Url:
DOI: 10.3201/eid1312.060559
PubMed: 18258063
PubMed Central: 2876737
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>Bartonella australis</italic> sp. nov. from Kangaroos, Australia</title><author><name sortKey="Fournier, Pierre Edouard" sort="Fournier, Pierre Edouard" uniqKey="Fournier P" first="Pierre-Edouard" last="Fournier">Pierre-Edouard Fournier</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Taylor, Carmel" sort="Taylor, Carmel" uniqKey="Taylor C" first="Carmel" last="Taylor">Carmel Taylor</name><affiliation><nlm:aff id="aff2">Queensland Health Scientific Services, Coopers Plains, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Rolain, Jean Marc" sort="Rolain, Jean Marc" uniqKey="Rolain J" first="Jean-Marc" last="Rolain">Jean-Marc Rolain</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Barrassi, Lina" sort="Barrassi, Lina" uniqKey="Barrassi L" first="Lina" last="Barrassi">Lina Barrassi</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Smith, Greg" sort="Smith, Greg" uniqKey="Smith G" first="Greg" last="Smith">Greg Smith</name><affiliation><nlm:aff id="aff2">Queensland Health Scientific Services, Coopers Plains, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18258063</idno><idno type="pmc">2876737</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2876737</idno><idno type="RBID">PMC:2876737</idno><idno type="doi">10.3201/eid1312.060559</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">001316</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001316</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>Bartonella australis</italic> sp. nov. from Kangaroos, Australia</title><author><name sortKey="Fournier, Pierre Edouard" sort="Fournier, Pierre Edouard" uniqKey="Fournier P" first="Pierre-Edouard" last="Fournier">Pierre-Edouard Fournier</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Taylor, Carmel" sort="Taylor, Carmel" uniqKey="Taylor C" first="Carmel" last="Taylor">Carmel Taylor</name><affiliation><nlm:aff id="aff2">Queensland Health Scientific Services, Coopers Plains, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Rolain, Jean Marc" sort="Rolain, Jean Marc" uniqKey="Rolain J" first="Jean-Marc" last="Rolain">Jean-Marc Rolain</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Barrassi, Lina" sort="Barrassi, Lina" uniqKey="Barrassi L" first="Lina" last="Barrassi">Lina Barrassi</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Smith, Greg" sort="Smith, Greg" uniqKey="Smith G" first="Greg" last="Smith">Greg Smith</name><affiliation><nlm:aff id="aff2">Queensland Health Scientific Services, Coopers Plains, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name><affiliation><nlm:aff id="aff1">Université de la Méditerranée, Marseille, France</nlm:aff></affiliation></author></analytic><series><title level="j">Emerging Infectious Diseases</title><idno type="ISSN">1080-6040</idno><idno type="eISSN">1080-6059</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="letter"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Emerg Infect Dis</journal-id><journal-id journal-id-type="publisher-id">EID</journal-id><journal-title-group><journal-title>Emerging Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">1080-6040</issn><issn pub-type="epub">1080-6059</issn><publisher><publisher-name>Centers for Disease Control and Prevention</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18258063</article-id><article-id pub-id-type="pmc">2876737</article-id><article-id pub-id-type="publisher-id">06-0559</article-id><article-id pub-id-type="doi">10.3201/eid1312.060559</article-id><article-categories><subj-group subj-group-type="heading"><subject>Letters to the Editor</subject></subj-group></article-categories><title-group><article-title><italic>Bartonella australis</italic> sp. nov. from Kangaroos, Australia</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Fournier</surname><given-names>Pierre-Edouard</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Taylor</surname><given-names>Carmel</given-names></name><xref ref-type="aff" rid="aff2">†</xref></contrib><contrib contrib-type="author"><name><surname>Rolain</surname><given-names>Jean-Marc</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Barrassi</surname><given-names>Lina</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Smith</surname><given-names>Greg</given-names></name><xref ref-type="aff" rid="aff2">†</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Raoult</surname><given-names>Didier</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><aff id="aff1"><label>*</label>Université de la Méditerranée, Marseille, France</aff><aff id="aff2"><label>†</label>Queensland Health Scientific Services, Coopers Plains, Queensland, Australia</aff></contrib-group><author-notes><corresp id="cor1">Address for correspondence: Didier Raoult, Unité des Rickettsies, IFR 48, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille CEDEX 05, France; email: <email xlink:href="didier.raoult@medecine.univ-mrs.fr">didier.raoult@medecine.univ-mrs.fr</email></corresp></author-notes><pub-date pub-type="ppub"><month>12</month><year>2007</year></pub-date><volume>13</volume><issue>12</issue><fpage>1961</fpage><lpage>1963</lpage><kwd-group kwd-group-type="author"><title>Keywords: </title><kwd><italic>Bartonella</italic></kwd><kwd><italic>australis</italic></kwd><kwd>Australia</kwd><kwd>kangaroo</kwd><kwd><italic>Macropus giganteus</italic></kwd><kwd>taxonomy</kwd><kwd>letter</kwd></kwd-group></article-meta></front><body><p><bold>To the Editor:</bold> During April–May 1999, 3 <italic>Bartonella</italic> isolates (AUST/NH1, AUST/NH2, AUST/NH3) were cultivated and established from the blood of 5 <italic>Macropus giganteus</italic> gray kangaroos from central coastal Queensland, Australia. We used multigene sequencing to evaluate whether these <italic>Bartonella</italic> isolates fulfill the minimum requirements for classification as a new species.</p><p>DNA from each <italic>Bartonella</italic> isolate was extracted by using the QIAamp tissue kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. Partial PCR amplification and sequencing of the genes encoding the 16S rDNA (<italic>rrs</italic>), citrate synthase (<italic>gltA</italic>), β-subunit of the RNA polymerase (<italic>rpoB</italic>), and cell division protein (<italic>ftsZ</italic>), as well as for the 16S–23S rDNA intergenic spacer (ITS) were attempted by using previously described primers and conditions (<xref ref-type="bibr" rid="R1"><italic>1</italic></xref>). <italic>Bartonella</italic> sp. isolates AUST/NH1 to AUST/NH3 exhibited identical sequences for all 4 genes and the spacer studied, and isolate AUST/NH1 was selected as type strain among kangaroo isolates. Similarity rates between strain Aust/NH1 and validated <italic>Bartonella</italic> species (<xref ref-type="local-data" rid="SD1">Appendix Table</xref>) ranged from 84.7% to 91.6%, from 97.5% to 98.5%, from 79.6% to 87.2%, from 85.4% to 95.0%, and from 83.5% to 87.1% for the ITS and <italic>rrs</italic>, <italic>gltA</italic>, <italic>rpoB,</italic> and <italic>ftsZ</italic> genes, respectively. Therefore, for each of these 4 genes or the spacer, strain AUST/NH1 exhibited similarity rates with all other species lower than the cutoffs published to classify <italic>Bartonella</italic> isolates within a validated species (<xref ref-type="bibr" rid="R1"><italic>1</italic></xref>). It may thus be regarded as a new species.</p><p>To estimate the genomic G+C content of strain AUST/NH1, we amplified and sequenced its <italic>ftsY</italic> gene as described (<xref ref-type="bibr" rid="R2"><italic>2</italic></xref>) by using the BartftsyF (5′-ATGACAAAAYCYTTTATMAA-3′) and BartftsyR (5′-TCATGAGTGTCTTCCTGC-3′) primers. The <italic>ftsY</italic> G+C content was 37.7%; the calculated genomic G+C content was 39.51%. The <italic>ftsY</italic> sequence was deposited in GenBank under accession no. DQ538398.</p><p>The phylogenetic relationships among the studied bartonellae were inferred from sequence alignments of each gene and from concatenated gene sequences by using the maximum parsimony and neighbor-joining methods within the MEGA version 2.1 software package (<xref ref-type="bibr" rid="R3"><italic>3</italic></xref>) and the maximum-likelihood method within the PHYLIP software package (<xref ref-type="bibr" rid="R4"><italic>4</italic></xref>). Using <italic>rrs</italic>, <italic>gltA</italic>, and <italic>rpoB</italic> sequences, the phylogenetic position of strain AUST/NH1 was supported by bootstrap values <70%. In contrast, by using the ITS, <italic>ftsZ,</italic> and concatenated sequences, strain AUST/NH1 clustered with a group of <italic>B. tribocorum</italic>, <italic>B. grahamii</italic>, and <italic>B. elizabethae</italic>, with elevated bootstrap values according to the 3 analysis methods (<xref ref-type="fig" rid="F1">Figure</xref>).</p><fig id="F1" fig-type="figure" position="float"><label>Figure</label><caption><p>Unrooted dendrogram showing the phylogenetic position of <italic>Bartonella</italic> sp. strain AUST/NH1 among <italic>Bartonella</italic> species inferred from the comparison of concatenated sequences from the <italic>rrs, gltA,</italic> intergenic spacer, <italic>rpoB,</italic> and <italic>ftsZ</italic> genes by the neighbor-joining method. We included only species for which all 5 genes were available. Bootstrap values are indicated at the nodes. The scale bar indicates nucleotide sequence divergence of 0.5%.</p></caption><graphic xlink:href="06-0559-F"></graphic></fig><p>The <italic>Bartonella</italic> strains we describe are the first, to our knowledge, obtained from kangaroos and, more generally, from marsupials. Before this study, the only 2 <italic>Bartonella</italic> species found in Australia were <italic>B. henselae</italic> (<xref ref-type="bibr" rid="R5"><italic>5</italic></xref>) and <italic>B. quintana</italic> (<xref ref-type="bibr" rid="R6"><italic>6</italic></xref>). We demonstrated that strain AUST/NH1 was reliably associated with a well-established cluster, including the rodent-associated <italic>B. elizabethae</italic>, <italic>B. grahamii</italic>, and <italic>B. tribocorum</italic> (<xref ref-type="bibr" rid="R7"><italic>7</italic></xref>). Therefore, we are confident that the phylogenic position of the new <italic>Bartonella,</italic> which was similar according to 3 analysis methods and supported by high bootstrap values, is reliable. Although <italic>B. grahamii</italic> (<xref ref-type="bibr" rid="R8"><italic>8</italic></xref>) and <italic>B. elizabethae</italic> (<xref ref-type="bibr" rid="R9"><italic>9</italic></xref>), members of the same phylogenetic cluster as strain AUST/NH1, cause human infections, the pathogenicity of <italic>B. tribocorum</italic> is as yet unknown. Its pathogenicity should therefore be investigated, especially for persons who come in contact with kangaroos.</p><p><italic>B. australis</italic> is a facultative intracellular gram-negative bacterium. It grows on Columbia agar with 5% sheep blood at 32°C to 37°C in a moist atmosphere containing 5% CO<sub>2</sub>. A primary culture was obtained after 7 days, and subculture was obtained after 4 days under the same conditions. Colonies are homogeneous, smooth, round, and gray-white. The 3 strains tested were oxidase negative, catalase negative, and nonmotile. Pathogenicity for humans is, as yet, unknown.</p><p>The type strain is strain AUST/NH1. The new species is distinguished from other <italic>Bartonella</italic> species by its 16S rRNA, <italic>gltA</italic>, <italic>rpoB</italic>, <italic>ftsZ</italic> gene sequences, as well as its 16S–23S rRNA ITS sequence. The estimated G+C content is 38%. The type strain exhibits a specific serotype (<xref ref-type="bibr" rid="R10"><italic>10</italic></xref>) and was susceptible to amoxicillin, ceftriaxone, imipenem, erythromycin, clarithromycin, ofloxacin, ciprofloxacin, rifampin, and tetracycline (unpub. data). The type strain AUST/NH1 has been deposited in the Collection of the World Health Organization Collaborative Center for Rickettsioses, Borrelioses and Tick-borne Infections (CSUR), Marseille, France, under reference CSUR B1; in the Collection de l’Institut Pasteur (CIP) under reference CIP 108978T; and in the Culture Collection of the University of Göteborg (CCUG), Sweden, under reference CCUG 51999. The strains AUST/NH2 and AUST/NH3 have been deposited in CSUR under references CSUR B2 and CSUR B3, in the CIP under references CIP 108980 and CIP 108979, and in CCUG under references CCUG 52000 and CCUG 52001, respectively.</p><sec sec-type="supplementary-material"><title>Supplementary Material</title><supplementary-material content-type="local-data" id="SD1"><caption><title>Appendix Table</title><p>Bartonella spp. and sequences used to validate Bartonella isolates (AUST/NH1, AUST/NH2, AUST/NH3) from 5 Macropus giganteus gray kangaroos, Australia, 1999*</p></caption><media xlink:href="06-0559_appT-s1.pdf" xlink:type="simple" id="d32e355" position="anchor" mimetype="application" mime-subtype="pdf"></media></supplementary-material></sec></body><back><fn-group><fn fn-type="citation"><p><italic>Suggested citation for this article</italic>: Fournier P-E, Taylor C, Rolain J-M, Barrassi L, Smith G, Raoult D. <italic>Bartonella australis</italic> sp. nov. from kangaroos, Australia [letter]. Emerg Infect Dis [serial on the Internet]. 2007 Dec [<italic>date cited</italic>]. Available from <ext-link ext-link-type="uri" xlink:href="http://www.cdc.gov/EID/content/13/12/1961.htm">http://www.cdc.gov/EID/content/13/12/1961.htm</ext-link></p></fn></fn-group><ack><title>Acknowledgments</title><p>We thank Annick Bernard, Laetitia Dorey, and Laurence Delaunay for technical help.</p></ack><ref-list><title>References</title><ref id="R1"><label>1. </label><mixed-citation publication-type="journal"><string-name><surname>La Scola</surname> <given-names>B</given-names></string-name>, <string-name><surname>Zeaiter</surname> <given-names>Z</given-names></string-name>, <string-name><surname>Khamis</surname> <given-names>A</given-names></string-name>, <string-name><surname>Raoult</surname> <given-names>D</given-names></string-name> <article-title>Gene-sequence-based criteria for species definition in bacteriology: the <italic>Bartonella</italic> paradigm.</article-title><source>Trends Microbiol</source> <year>2003</year>;<volume>11</volume>:<fpage>318</fpage>–<lpage>21</lpage> <pub-id pub-id-type="doi">10.1016/S0966-842X(03)00143-4</pub-id><pub-id pub-id-type="pmid">12875815</pub-id></mixed-citation></ref><ref id="R2"><label>2. </label><mixed-citation publication-type="journal"><string-name><surname>Fournier</surname> <given-names>PE</given-names></string-name>, <string-name><surname>Suhre</surname> <given-names>K</given-names></string-name>, <string-name><surname>Fournous</surname> <given-names>G</given-names></string-name>, <string-name><surname>Raoult</surname> <given-names>D</given-names></string-name> <article-title>Estimation of prokaryote genomic G+C content by sequencing universally-conserved genes.</article-title><source>Int J Syst Evol Microbiol</source> <year>2006</year>;<volume>56</volume>:<fpage>1205</fpage>–<lpage>9</lpage></mixed-citation></ref><ref id="R3"><label>3. </label><mixed-citation publication-type="journal"><string-name><surname>Kumar</surname> <given-names>S</given-names></string-name>, <string-name><surname>Tamura</surname> <given-names>K</given-names></string-name>, <string-name><surname>Jakobsen</surname> <given-names>IB</given-names></string-name>, <string-name><surname>Nei</surname> <given-names>M</given-names></string-name> <article-title>MEGA2: molecular evolutionary genetics analysis software.</article-title><source>Bioinformatics</source> <year>2001</year>;<volume>17</volume>:<fpage>1244</fpage>–<lpage>5</lpage> <pub-id pub-id-type="doi">10.1093/bioinformatics/17.12.1244</pub-id><pub-id pub-id-type="pmid">11751241</pub-id></mixed-citation></ref><ref id="R4"><label>4. </label><mixed-citation publication-type="journal"><string-name><surname>Retief</surname> <given-names>JD</given-names></string-name> <article-title>Phylogenetic analysis using PHYLIP.</article-title><source>Methods Mol Biol</source> <year>2000</year>;<volume>132</volume>:<fpage>243</fpage>–<lpage>58</lpage><pub-id pub-id-type="pmid">10547839</pub-id></mixed-citation></ref><ref id="R5"><label>5. </label><mixed-citation publication-type="journal"><string-name><surname>Fournier</surname> <given-names>PE</given-names></string-name>, <string-name><surname>Robson</surname> <given-names>J</given-names></string-name>, <string-name><surname>Zeaiter</surname> <given-names>Z</given-names></string-name>, <string-name><surname>McDougall</surname> <given-names>R</given-names></string-name>, <string-name><surname>Byrne</surname> <given-names>S</given-names></string-name>, <string-name><surname>Raoult</surname> <given-names>D</given-names></string-name> <article-title>Improved culture from lymph nodes of patients with cat scratch disease and genotypic characterization of <italic>Bartonella henselae</italic> isolates in Australia.</article-title><source>J Clin Microbiol</source> <year>2002</year>;<volume>40</volume>:<fpage>3620</fpage>–<lpage>4</lpage> <pub-id pub-id-type="doi">10.1128/JCM.40.10.3620-3624.2002</pub-id><pub-id pub-id-type="pmid">12354855</pub-id></mixed-citation></ref><ref id="R6"><label>6. </label><mixed-citation publication-type="journal"><string-name><surname>Rathbone</surname> <given-names>P</given-names></string-name>, <string-name><surname>Graves</surname> <given-names>S</given-names></string-name>, <string-name><surname>Miller</surname> <given-names>D</given-names></string-name>, <string-name><surname>Odorico</surname> <given-names>D</given-names></string-name>, <string-name><surname>Jones</surname> <given-names>S</given-names></string-name>, <string-name><surname>Hellyar</surname> <given-names>A</given-names></string-name>, <etal></etal><article-title><italic>Bartonella (Rochalimaea) quintana</italic> causing fever and bacteremia in an immunocompromised patient with non-Hodgkin's lymphoma.</article-title><source>Pathology</source> <year>1996</year>;<volume>28</volume>:<fpage>80</fpage>–<lpage>3</lpage> <pub-id pub-id-type="doi">10.1080/00313029600169593</pub-id><pub-id pub-id-type="pmid">8714279</pub-id></mixed-citation></ref><ref id="R7"><label>7. </label><mixed-citation publication-type="journal"><string-name><surname>Houpikian</surname> <given-names>P</given-names></string-name>, <string-name><surname>Raoult</surname> <given-names>D</given-names></string-name> <article-title>Molecular phylogeny of the genus <italic>Bartonella</italic>: what is the current knowledge?</article-title><source>FEMS Microbiol Lett</source> <year>2001</year>;<volume>200</volume>:<fpage>1</fpage>–<lpage>7</lpage> <pub-id pub-id-type="doi">10.1111/j.1574-6968.2001.tb10684.x</pub-id><pub-id pub-id-type="pmid">11410341</pub-id></mixed-citation></ref><ref id="R8"><label>8. </label><mixed-citation publication-type="journal"><string-name><surname>Kerkhoff</surname> <given-names>FT</given-names></string-name>, <string-name><surname>Bergmans</surname> <given-names>AMC</given-names></string-name>, <string-name><surname>van der Zee</surname> <given-names>A</given-names></string-name>, <string-name><surname>Rothova</surname> <given-names>A</given-names></string-name> <article-title>Demonstration of <italic>Bartonella grahamii</italic> DNA in ocular fluids of a patient with neuroretinitis.</article-title><source>J Clin Microbiol</source> <year>1999</year>;<volume>37</volume>:<fpage>4034</fpage>–<lpage>8</lpage><pub-id pub-id-type="pmid">10565926</pub-id></mixed-citation></ref><ref id="R9"><label>9. </label><mixed-citation publication-type="journal"><string-name><surname>Daly</surname> <given-names>JS</given-names></string-name>, <string-name><surname>Worthington</surname> <given-names>MG</given-names></string-name>, <string-name><surname>Brenner</surname> <given-names>DJ</given-names></string-name>, <string-name><surname>Moss</surname> <given-names>WC</given-names></string-name>, <string-name><surname>Hollis</surname> <given-names>DG</given-names></string-name>, <string-name><surname>Weyant</surname> <given-names>RS</given-names></string-name>, <etal></etal><article-title><italic>Rochalimaea elizabethae</italic> sp. nov. isolated from a patient with endocarditis.</article-title><source>J Clin Microbiol</source> <year>1993</year>;<volume>31</volume>:<fpage>872</fpage>–<lpage>81</lpage><pub-id pub-id-type="pmid">7681847</pub-id></mixed-citation></ref><ref id="R10"><label>10. </label><mixed-citation publication-type="journal"><string-name><surname>Bonhomme</surname> <given-names>CJ</given-names></string-name>, <string-name><surname>Nappez</surname> <given-names>C</given-names></string-name>, <string-name><surname>Raoult</surname> <given-names>D</given-names></string-name> <article-title>Microarray for serotyping of <italic>Bartonella</italic> species.</article-title><source>BMC Microbiol</source> <year>2007</year>;<volume>7</volume>:<fpage>59</fpage> <pub-id pub-id-type="doi">10.1186/1471-2180-7-59</pub-id><pub-id 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| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |