Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cell Wall Modifications in Arabidopsis Plants with Altered <italic>α</italic>-<sc>l</sc>-Arabinofuranosidase Activity<xref ref-type="fn" rid="fn3">[C]</xref><xref ref-type="fn" rid="fn4">[W]</xref></title><author><name sortKey="Chavez Montes, Ricardo A" sort="Chavez Montes, Ricardo A" uniqKey="Chavez Montes R" first="Ricardo A." last="Chávez Montes">Ricardo A. Chávez Montes</name></author><author><name sortKey="Ranocha, Philippe" sort="Ranocha, Philippe" uniqKey="Ranocha P" first="Philippe" last="Ranocha">Philippe Ranocha</name></author><author><name sortKey="Martinez, Yves" sort="Martinez, Yves" uniqKey="Martinez Y" first="Yves" last="Martinez">Yves Martinez</name></author><author><name sortKey="Minic, Zoran" sort="Minic, Zoran" uniqKey="Minic Z" first="Zoran" last="Minic">Zoran Minic</name></author><author><name sortKey="Jouanin, Lise" sort="Jouanin, Lise" uniqKey="Jouanin L" first="Lise" last="Jouanin">Lise Jouanin</name></author><author><name sortKey="Marquis, Melanie" sort="Marquis, Melanie" uniqKey="Marquis M" first="Mélanie" last="Marquis">Mélanie Marquis</name></author><author><name sortKey="Saulnier, Luc" sort="Saulnier, Luc" uniqKey="Saulnier L" first="Luc" last="Saulnier">Luc Saulnier</name></author><author><name sortKey="Fulton, Lynette M" sort="Fulton, Lynette M" uniqKey="Fulton L" first="Lynette M." last="Fulton">Lynette M. Fulton</name></author><author><name sortKey="Cobbett, Christopher S" sort="Cobbett, Christopher S" uniqKey="Cobbett C" first="Christopher S." last="Cobbett">Christopher S. Cobbett</name></author><author><name sortKey="Bitton, Frederique" sort="Bitton, Frederique" uniqKey="Bitton F" first="Frédérique" last="Bitton">Frédérique Bitton</name></author><author><name sortKey="Renou, Jean Pierre" sort="Renou, Jean Pierre" uniqKey="Renou J" first="Jean-Pierre" last="Renou">Jean-Pierre Renou</name></author><author><name sortKey="Jauneau, Alain" sort="Jauneau, Alain" uniqKey="Jauneau A" first="Alain" last="Jauneau">Alain Jauneau</name></author><author><name sortKey="Goffner, Deborah" sort="Goffner, Deborah" uniqKey="Goffner D" first="Deborah" last="Goffner">Deborah Goffner</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18344421</idno><idno type="pmc">2330305</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330305</idno><idno type="RBID">PMC:2330305</idno><idno type="doi">10.1104/pp.107.110023</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">001053</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001053</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cell Wall Modifications in Arabidopsis Plants with Altered <italic>α</italic>-<sc>l</sc>-Arabinofuranosidase Activity<xref ref-type="fn" rid="fn3">[C]</xref><xref ref-type="fn" rid="fn4">[W]</xref></title><author><name sortKey="Chavez Montes, Ricardo A" sort="Chavez Montes, Ricardo A" uniqKey="Chavez Montes R" first="Ricardo A." last="Chávez Montes">Ricardo A. Chávez Montes</name></author><author><name sortKey="Ranocha, Philippe" sort="Ranocha, Philippe" uniqKey="Ranocha P" first="Philippe" last="Ranocha">Philippe Ranocha</name></author><author><name sortKey="Martinez, Yves" sort="Martinez, Yves" uniqKey="Martinez Y" first="Yves" last="Martinez">Yves Martinez</name></author><author><name sortKey="Minic, Zoran" sort="Minic, Zoran" uniqKey="Minic Z" first="Zoran" last="Minic">Zoran Minic</name></author><author><name sortKey="Jouanin, Lise" sort="Jouanin, Lise" uniqKey="Jouanin L" first="Lise" last="Jouanin">Lise Jouanin</name></author><author><name sortKey="Marquis, Melanie" sort="Marquis, Melanie" uniqKey="Marquis M" first="Mélanie" last="Marquis">Mélanie Marquis</name></author><author><name sortKey="Saulnier, Luc" sort="Saulnier, Luc" uniqKey="Saulnier L" first="Luc" last="Saulnier">Luc Saulnier</name></author><author><name sortKey="Fulton, Lynette M" sort="Fulton, Lynette M" uniqKey="Fulton L" first="Lynette M." last="Fulton">Lynette M. Fulton</name></author><author><name sortKey="Cobbett, Christopher S" sort="Cobbett, Christopher S" uniqKey="Cobbett C" first="Christopher S." last="Cobbett">Christopher S. Cobbett</name></author><author><name sortKey="Bitton, Frederique" sort="Bitton, Frederique" uniqKey="Bitton F" first="Frédérique" last="Bitton">Frédérique Bitton</name></author><author><name sortKey="Renou, Jean Pierre" sort="Renou, Jean Pierre" uniqKey="Renou J" first="Jean-Pierre" last="Renou">Jean-Pierre Renou</name></author><author><name sortKey="Jauneau, Alain" sort="Jauneau, Alain" uniqKey="Jauneau A" first="Alain" last="Jauneau">Alain Jauneau</name></author><author><name sortKey="Goffner, Deborah" sort="Goffner, Deborah" uniqKey="Goffner D" first="Deborah" last="Goffner">Deborah Goffner</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Although cell wall remodeling is an essential feature of plant growth and development, the underlying molecular mechanisms are poorly understood. This work describes the characterization of Arabidopsis (<italic>Arabidopsis thaliana</italic>) plants with altered expression of ARAF1, a bifunctional <italic>α</italic>-<sc>l</sc>-arabinofuranosidase/<italic>β</italic>-<sc>d</sc>-xylosidase (At3g10740) belonging to family 51 glycosyl-hydrolases. <italic>ARAF1</italic> was localized in several cell types in the vascular system of roots and stems, including xylem vessels and parenchyma cells surrounding the vessels, the cambium, and the phloem. <italic>araf1</italic> T-DNA insertional mutants showed no visible phenotype, whereas transgenic plants that overexpressed ARAF1 exhibited a delay in inflorescence emergence and altered stem architecture. Although global monosaccharide analysis indicated only slight differences in cell wall composition in both mutant and overexpressing lines, immunolocalization experiments using anti-arabinan (LM6) and anti-xylan (LM10) antibodies indicated cell type-specific alterations in cell wall structure. In <italic>araf1</italic> mutants, an increase in LM6 signal intensity was observed in the phloem, cambium, and xylem parenchyma in stems and roots, largely coinciding with <italic>ARAF1</italic> expression sites. The ectopic overexpression of ARAF1 resulted in an increase in LM10 labeling in the secondary walls of interfascicular fibers and xylem vessels. The combined <italic>ARAF1</italic> gene expression and immunolocalization studies suggest that arabinan-containing pectins are potential in vivo substrates of ARAF1 in Arabidopsis.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">plantphysiol</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18344421</article-id><article-id pub-id-type="pmc">2330305</article-id><article-id pub-id-type="publisher-id">110023</article-id><article-id pub-id-type="doi">10.1104/pp.107.110023</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Biochemical Processes and Macromolecular Structures</subject></subj-group></subj-group></article-categories><title-group><article-title>Cell Wall Modifications in Arabidopsis Plants with Altered <italic>α</italic>-<sc>l</sc>-Arabinofuranosidase Activity<xref ref-type="fn" rid="fn3">[C]</xref><xref ref-type="fn" rid="fn4">[W]</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chávez Montes</surname><given-names>Ricardo A.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ranocha</surname><given-names>Philippe</given-names></name></contrib><contrib contrib-type="author"><name><surname>Martinez</surname><given-names>Yves</given-names></name></contrib><contrib contrib-type="author"><name><surname>Minic</surname><given-names>Zoran</given-names></name><xref ref-type="fn" rid="fn1">1</xref></contrib><contrib contrib-type="author"><name><surname>Jouanin</surname><given-names>Lise</given-names></name></contrib><contrib contrib-type="author"><name><surname>Marquis</surname><given-names>Mélanie</given-names></name></contrib><contrib contrib-type="author"><name><surname>Saulnier</surname><given-names>Luc</given-names></name></contrib><contrib contrib-type="author"><name><surname>Fulton</surname><given-names>Lynette M.</given-names></name><xref ref-type="fn" rid="fn2">2</xref></contrib><contrib contrib-type="author"><name><surname>Cobbett</surname><given-names>Christopher S.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bitton</surname><given-names>Frédérique</given-names></name></contrib><contrib contrib-type="author"><name><surname>Renou</surname><given-names>Jean-Pierre</given-names></name></contrib><contrib contrib-type="author"><name><surname>Jauneau</surname><given-names>Alain</given-names></name></contrib><contrib contrib-type="author"><name><surname>Goffner</surname><given-names>Deborah</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="N0x1c0e740N0x437b6c8">UMR 5546, CNRS-Université Paul Sabatier, Surfaces Cellulaires et Signalisation chez les Végétaux, BP 42617 Auzeville, 31326 Castanet-Tolosan, France (R.A.C.M., P.R., Y.M., A.J., D.G.); Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles cedex, France (Z.M., L.J.); Biopolymères Interactions Assemblages, Unité de Recherche sur les Polysaccharides leurs Organisations et Interactions, Institut National de la Recherche Agronomique, BP 71627, 44316 Nantes cedex 03, France (M.M., L.S.); Department of Genetics, University of Melbourne, Victoria 3010, Australia (L.M.F., C.S.C.); and Unité de Recherche en Génomique Végétale, INRA-CNRS, CP 5708, 91057 Evry cedex, France (F.B., J.-P.R.)</aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author; e-mail <email>goffner@scsv.ups-tlse.fr</email>.</p></fn><fn id="fn1"><label>1</label><p>Present address: Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada.</p></fn><fn id="fn2"><label>2</label><p>Present address: Entwicklungsbiologie der Pflanzen, Wissenschaftszentrum Weihenstephan, Technische Universität Munich, Am Hochanger 4, D–85354 Freising, Germany.</p></fn></author-notes><pub-date pub-type="ppub"><month>5</month><year>2008</year></pub-date><volume>147</volume><issue>1</issue><fpage>63</fpage><lpage>77</lpage><history><date date-type="received"><day>28</day><month>9</month><year>2007</year></date><date date-type="accepted"><day>13</day><month>3</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2008, American Society of Plant Biologists</copyright-statement></permissions><abstract><p>Although cell wall remodeling is an essential feature of plant growth and development, the underlying molecular mechanisms are poorly understood. This work describes the characterization of Arabidopsis (<italic>Arabidopsis thaliana</italic>) plants with altered expression of ARAF1, a bifunctional <italic>α</italic>-<sc>l</sc>-arabinofuranosidase/<italic>β</italic>-<sc>d</sc>-xylosidase (At3g10740) belonging to family 51 glycosyl-hydrolases. <italic>ARAF1</italic> was localized in several cell types in the vascular system of roots and stems, including xylem vessels and parenchyma cells surrounding the vessels, the cambium, and the phloem. <italic>araf1</italic> T-DNA insertional mutants showed no visible phenotype, whereas transgenic plants that overexpressed ARAF1 exhibited a delay in inflorescence emergence and altered stem architecture. Although global monosaccharide analysis indicated only slight differences in cell wall composition in both mutant and overexpressing lines, immunolocalization experiments using anti-arabinan (LM6) and anti-xylan (LM10) antibodies indicated cell type-specific alterations in cell wall structure. In <italic>araf1</italic> mutants, an increase in LM6 signal intensity was observed in the phloem, cambium, and xylem parenchyma in stems and roots, largely coinciding with <italic>ARAF1</italic> expression sites. The ectopic overexpression of ARAF1 resulted in an increase in LM10 labeling in the secondary walls of interfascicular fibers and xylem vessels. The combined <italic>ARAF1</italic> gene expression and immunolocalization studies suggest that arabinan-containing pectins are potential in vivo substrates of ARAF1 in Arabidopsis.</p></abstract></article-meta><notes><fn-group><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Deborah Goffner (<email>goffner@scsv.ups-tlse.fr</email>).</p></fn><fn id="fn3"><label>[C]</label><p>Some figures in this article are displayed in color online but in black and white in the print edition.</p></fn><fn id="fn4"><label>[W]</label><p>The online version of this article contains Web-only data.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org/cgi/doi/10.1104/pp.107.110023">www.plantphysiol.org/cgi/doi/10.1104/pp.107.110023</ext-link></p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0010539 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0010539 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.33. |  |