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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of Cortical Arrays from <italic>Tradescantia virginiana</italic> at High Resolution Reveals Discrete Microtubule Subpopulations and Demonstrates That Confocal Images of Arrays Can Be Misleading<xref ref-type="fn" rid="fn1">[W]</xref></title><author><name sortKey="Barton, Deborah A" sort="Barton, Deborah A" uniqKey="Barton D" first="Deborah A." last="Barton">Deborah A. Barton</name><affiliation><nlm:aff id="aff1">School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia</nlm:aff></affiliation></author><author><name sortKey="Vantard, Marylin" sort="Vantard, Marylin" uniqKey="Vantard M" first="Marylin" last="Vantard">Marylin Vantard</name><affiliation><nlm:aff id="aff2">Département Réponse et Dynamique Cellulaires, Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche 5168, Centre National de la Recherche Scientifique/Commissariat à l'Energie Atomique/Institut National de la Recherche Agronomique/Université Joseph Fourier de Grenoble, F-38054 Grenoble, France</nlm:aff></affiliation></author><author><name sortKey="Overall, Robyn L" sort="Overall, Robyn L" uniqKey="Overall R" first="Robyn L." last="Overall">Robyn L. Overall</name><affiliation><nlm:aff id="aff1">School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18430803</idno><idno type="pmc">2390730</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2390730</idno><idno type="RBID">PMC:2390730</idno><idno type="doi">10.1105/tpc.108.058503</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">001042</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001042</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Analysis of Cortical Arrays from <italic>Tradescantia virginiana</italic> at High Resolution Reveals Discrete Microtubule Subpopulations and Demonstrates That Confocal Images of Arrays Can Be Misleading<xref ref-type="fn" rid="fn1">[W]</xref></title><author><name sortKey="Barton, Deborah A" sort="Barton, Deborah A" uniqKey="Barton D" first="Deborah A." last="Barton">Deborah A. Barton</name><affiliation><nlm:aff id="aff1">School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia</nlm:aff></affiliation></author><author><name sortKey="Vantard, Marylin" sort="Vantard, Marylin" uniqKey="Vantard M" first="Marylin" last="Vantard">Marylin Vantard</name><affiliation><nlm:aff id="aff2">Département Réponse et Dynamique Cellulaires, Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche 5168, Centre National de la Recherche Scientifique/Commissariat à l'Energie Atomique/Institut National de la Recherche Agronomique/Université Joseph Fourier de Grenoble, F-38054 Grenoble, France</nlm:aff></affiliation></author><author><name sortKey="Overall, Robyn L" sort="Overall, Robyn L" uniqKey="Overall R" first="Robyn L." last="Overall">Robyn L. Overall</name><affiliation><nlm:aff id="aff1">School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia</nlm:aff></affiliation></author></analytic><series><title level="j">The Plant Cell</title><idno type="ISSN">1040-4651</idno><idno type="eISSN">1532-298X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cortical microtubule arrays are highly organized networks involved in directing cellulose microfibril deposition within the cell wall. Their organization results from complex interactions between individual microtubules and microtubule-associated proteins. The precise details of these interactions are often not evident using optical microscopy. Using high-resolution scanning electron microscopy, we analyzed extensive regions of cortical arrays and identified two spatially discrete microtubule subpopulations that exhibited different stabilities. Microtubules that lay adjacent to the plasma membrane were often bundled and more stable than the randomly aligned, discordant microtubules that lay deeper in the cytoplasm. Immunolabeling revealed katanin at microtubule ends, on curves, or at sites along microtubules in line with neighboring microtubule ends. End binding 1 protein also localized along microtubules, at microtubule ends or junctions between microtubules, and on the plasma membrane in direct line with microtubule ends. We show fine bands in vivo that traverse and may encircle microtubules. Comparing confocal and electron microscope images of fluorescently tagged arrays, we demonstrate that optical images are misleading, highlighting the fundamental importance of studying cortical microtubule arrays at high resolution.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id><journal-id journal-id-type="publisher-id">plantcell</journal-id><journal-title>The Plant Cell</journal-title><issn pub-type="ppub">1040-4651</issn><issn pub-type="epub">1532-298X</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18430803</article-id><article-id pub-id-type="pmc">2390730</article-id><article-id pub-id-type="publisher-id">058503</article-id><article-id pub-id-type="doi">10.1105/tpc.108.058503</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject></subj-group></article-categories><title-group><article-title>Analysis of Cortical Arrays from <italic>Tradescantia virginiana</italic> at High Resolution Reveals Discrete Microtubule Subpopulations and Demonstrates That Confocal Images of Arrays Can Be Misleading<xref ref-type="fn" rid="fn1">[W]</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Barton</surname><given-names>Deborah A.</given-names></name><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Vantard</surname><given-names>Marylin</given-names></name><xref ref-type="aff" rid="aff2">b</xref></contrib><contrib contrib-type="author"><name><surname>Overall</surname><given-names>Robyn L.</given-names></name><xref ref-type="aff" rid="aff1">a</xref><xref ref-type="corresp" rid="cor1">1</xref></contrib></contrib-group><aff id="aff1"><label>a</label>School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia</aff><aff id="aff2"><label>b</label>Département Réponse et Dynamique Cellulaires, Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche 5168, Centre National de la Recherche Scientifique/Commissariat à l'Energie Atomique/Institut National de la Recherche Agronomique/Université Joseph Fourier de Grenoble, F-38054 Grenoble, France</aff><author-notes><fn id="cor1"><label>1</label><p>Address correspondence to <email>roverall@mail.usyd.edu.au</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2008</year></pub-date><volume>20</volume><issue>4</issue><fpage>982</fpage><lpage>994</lpage><history><date date-type="received"><day>5</day><month>2</month><year>2008</year></date><date date-type="rev-recd"><day>30</day><month>3</month><year>2008</year></date><date date-type="accepted"><day>7</day><month>4</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2008, American Society of Plant Biologists</copyright-statement></permissions><abstract><p>Cortical microtubule arrays are highly organized networks involved in directing cellulose microfibril deposition within the cell wall. Their organization results from complex interactions between individual microtubules and microtubule-associated proteins. The precise details of these interactions are often not evident using optical microscopy. Using high-resolution scanning electron microscopy, we analyzed extensive regions of cortical arrays and identified two spatially discrete microtubule subpopulations that exhibited different stabilities. Microtubules that lay adjacent to the plasma membrane were often bundled and more stable than the randomly aligned, discordant microtubules that lay deeper in the cytoplasm. Immunolabeling revealed katanin at microtubule ends, on curves, or at sites along microtubules in line with neighboring microtubule ends. End binding 1 protein also localized along microtubules, at microtubule ends or junctions between microtubules, and on the plasma membrane in direct line with microtubule ends. We show fine bands in vivo that traverse and may encircle microtubules. Comparing confocal and electron microscope images of fluorescently tagged arrays, we demonstrate that optical images are misleading, highlighting the fundamental importance of studying cortical microtubule arrays at high resolution.</p></abstract></article-meta><notes><fn-group><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantcell.org">www.plantcell.org</ext-link>) is: Robyn L. Overall (<email>roverall@mail.usyd.edu.au</email>).</p></fn><fn id="fn1"><label>[W]</label><p>Online version contains Web-only data.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="www.plantcell.org/cgi/doi/10.1105/tpc.108.058503">www.plantcell.org/cgi/doi/10.1105/tpc.108.058503</ext-link></p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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