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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing <italic>Escherichia coli</italic> Strains Isolated from Healthy Infants in Germany and Australia </title><author><name sortKey="Beutin, Lothar" sort="Beutin, Lothar" uniqKey="Beutin L" first="Lothar" last="Beutin">Lothar Beutin</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Marches, Olivier" sort="Marches, Olivier" uniqKey="Marches O" first="Olivier" last="Marchés">Olivier Marchés</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bettelheim, Karl A" sort="Bettelheim, Karl A" uniqKey="Bettelheim K" first="Karl A." last="Bettelheim">Karl A. Bettelheim</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Gleier, Kerstin" sort="Gleier, Kerstin" uniqKey="Gleier K" first="Kerstin" last="Gleier">Kerstin Gleier</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zimmermann, Sonja" sort="Zimmermann, Sonja" uniqKey="Zimmermann S" first="Sonja" last="Zimmermann">Sonja Zimmermann</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Schmidt, Herbert" sort="Schmidt, Herbert" uniqKey="Schmidt H" first="Herbert" last="Schmidt">Herbert Schmidt</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Oswald, Eric" sort="Oswald, Eric" uniqKey="Oswald E" first="Eric" last="Oswald">Eric Oswald</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12819087</idno><idno type="pmc">162008</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC162008</idno><idno type="RBID">PMC:162008</idno><idno type="doi">10.1128/IAI.71.7.3995-4002.2003</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">000F90</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F90</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing <italic>Escherichia coli</italic> Strains Isolated from Healthy Infants in Germany and Australia </title><author><name sortKey="Beutin, Lothar" sort="Beutin, Lothar" uniqKey="Beutin L" first="Lothar" last="Beutin">Lothar Beutin</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Marches, Olivier" sort="Marches, Olivier" uniqKey="Marches O" first="Olivier" last="Marchés">Olivier Marchés</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bettelheim, Karl A" sort="Bettelheim, Karl A" uniqKey="Bettelheim K" first="Karl A." last="Bettelheim">Karl A. Bettelheim</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Gleier, Kerstin" sort="Gleier, Kerstin" uniqKey="Gleier K" first="Kerstin" last="Gleier">Kerstin Gleier</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zimmermann, Sonja" sort="Zimmermann, Sonja" uniqKey="Zimmermann S" first="Sonja" last="Zimmermann">Sonja Zimmermann</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Schmidt, Herbert" sort="Schmidt, Herbert" uniqKey="Schmidt H" first="Herbert" last="Schmidt">Herbert Schmidt</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Oswald, Eric" sort="Oswald, Eric" uniqKey="Oswald E" first="Eric" last="Oswald">Eric Oswald</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Infection and Immunity</title><idno type="ISSN">0019-9567</idno><idno type="eISSN">1098-5522</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Fecal samples from healthy children under 2 years of age living in Berlin, Germany (205 infants), and Melbourne, Australia (184 infants), were investigated for the presence of attaching and effacing (AE) <italic>Escherichia coli</italic> (AEEC) strains by screening for <italic>eae</italic> (intimin) genes. Twenty-seven AEEC strains were isolated from 14 children (7.6%) from Melbourne and from 12 children (5.9%) from Berlin. The 27 AEEC strains were classified as enterohemorrhagic <italic>E. coli</italic> (one strain, producing Shiga toxin 1), typical enteropathogenic <italic>E. coli</italic> (EPEC) (one strain carrying an EPEC adherence factor [EAF] plasmid), and atypical EPEC (25 strains negative for Shiga toxins and EAF plasmids). The AEEC were divided into 18 different serotypes, O-nontypeable and O-rough strains. Typing of their intimin genes revealed the presence of intimin α in 6 strains, intimin β in 11 strains, intimin γ in 7 strains, intimin ζ in 2 strains, and intimin η in one strain. Analysis of HEp-2 cell adherence showed diffuse adherence or localized adherence-like patterns in 26 AEEC strains; local adherence was found only with the EAF-positive strain. Ten AEEC strains showed an AE property with the fluorescent actin staining (FAS) test. The introduction of an EAF plasmid (pMAR7) converted 11 FAS-negative AEEC strains to FAS positive and increased the FAS reaction in six FAS-positive AEEC strains, indicating that the genes needed for the AE phenotype were functional in these strains. Our finding indicates that atypical EPEC strains could play a double role as strains that naturally immunize against intimin in humans and as reservoirs for new emerging human pathogenic EPEC strains.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Immun</journal-id><journal-id journal-id-type="publisher-id">iai</journal-id><journal-title>Infection and Immunity</journal-title><issn pub-type="ppub">0019-9567</issn><issn pub-type="epub">1098-5522</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12819087</article-id><article-id pub-id-type="pmc">162008</article-id><article-id pub-id-type="publisher-id">1486</article-id><article-id pub-id-type="doi">10.1128/IAI.71.7.3995-4002.2003</article-id><article-categories><subj-group subj-group-type="heading"><subject>Bacterial Infections</subject></subj-group></article-categories><title-group><article-title>HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing <italic>Escherichia coli</italic> Strains Isolated from Healthy Infants in Germany and Australia </article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Beutin</surname><given-names>Lothar</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Marchés</surname><given-names>Olivier</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Bettelheim</surname><given-names>Karl A.</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Gleier</surname><given-names>Kerstin</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zimmermann</surname><given-names>Sonja</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Schmidt</surname><given-names>Herbert</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Oswald</surname><given-names>Eric</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib></contrib-group><aff id="aff1">Division of Emerging Bacterial Pathogens, Department of Biological Safety, Robert Koch Institute, 13353 Berlin,<label>1</label> Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden Germany;,<label>4</label> Laboratoire associé INRA/ENVT de Microbiologie Moléculaire, Ecole Nationale Vétérinaire, 31000 Toulouse, France,<label>2</label> Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3052, Australia<label>3</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Department of Biological Safety (ZBS3), Robert Koch Institut, Nordufer 20, D-13353 Berlin, Germany. Phone: 49 30 45 47 2484. Fax: 49 30 45 47 2673. E-mail: <email>BeutinL@rki.de</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>7</month><year>2003</year></pub-date><volume>71</volume><issue>7</issue><fpage>3995</fpage><lpage>4002</lpage><history><date date-type="received"><day>15</day><month>10</month><year>2002</year></date><date date-type="rev-recd"><day>28</day><month>1</month><year>2003</year></date><date date-type="accepted"><day>6</day><month>4</month><year>2003</year></date></history><copyright-statement>Copyright © 2003, American Society for Microbiology</copyright-statement><copyright-year>2003</copyright-year><abstract><p>Fecal samples from healthy children under 2 years of age living in Berlin, Germany (205 infants), and Melbourne, Australia (184 infants), were investigated for the presence of attaching and effacing (AE) <italic>Escherichia coli</italic> (AEEC) strains by screening for <italic>eae</italic> (intimin) genes. Twenty-seven AEEC strains were isolated from 14 children (7.6%) from Melbourne and from 12 children (5.9%) from Berlin. The 27 AEEC strains were classified as enterohemorrhagic <italic>E. coli</italic> (one strain, producing Shiga toxin 1), typical enteropathogenic <italic>E. coli</italic> (EPEC) (one strain carrying an EPEC adherence factor [EAF] plasmid), and atypical EPEC (25 strains negative for Shiga toxins and EAF plasmids). The AEEC were divided into 18 different serotypes, O-nontypeable and O-rough strains. Typing of their intimin genes revealed the presence of intimin α in 6 strains, intimin β in 11 strains, intimin γ in 7 strains, intimin ζ in 2 strains, and intimin η in one strain. Analysis of HEp-2 cell adherence showed diffuse adherence or localized adherence-like patterns in 26 AEEC strains; local adherence was found only with the EAF-positive strain. Ten AEEC strains showed an AE property with the fluorescent actin staining (FAS) test. The introduction of an EAF plasmid (pMAR7) converted 11 FAS-negative AEEC strains to FAS positive and increased the FAS reaction in six FAS-positive AEEC strains, indicating that the genes needed for the AE phenotype were functional in these strains. Our finding indicates that atypical EPEC strains could play a double role as strains that naturally immunize against intimin in humans and as reservoirs for new emerging human pathogenic EPEC strains.</p></abstract></article-meta><notes><fn-group><fn><p><italic>Editor:</italic> V. J. DiRita</p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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