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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of <italic>Plasmodium falciparum</italic> chromosomes</title><author><name sortKey="O Onnell, Rebecca A" sort="O Onnell, Rebecca A" uniqKey="O Onnell R" first="Rebecca A." last="O Onnell">Rebecca A. O Onnell</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Department of Microbiology & Immunology and the Cooperative Research Centre for Vaccine Technology, The University of Melbourne, Victoria 3010, Australia,</nlm:aff></affiliation></author><author><name sortKey="Freitas Junior, Lucio H" sort="Freitas Junior, Lucio H" uniqKey="Freitas Junior L" first="Lúcio H." last="Freitas-Junior">Lúcio H. Freitas-Junior</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 1960, Institut Pasteur, F-75724 Paris Cedex 15, France,</nlm:aff></affiliation></author><author><name sortKey="Preiser, Peter R" sort="Preiser, Peter R" uniqKey="Preiser P" first="Peter R." last="Preiser">Peter R. Preiser</name><affiliation><nlm:aff wicri:cut=" and" id="N0x9a175e8.0x98b8888">National Institute of Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</nlm:aff></affiliation></author><author><name sortKey="Williamson, Donald H" sort="Williamson, Donald H" uniqKey="Williamson D" first="Donald H." last="Williamson">Donald H. Williamson</name><affiliation><nlm:aff wicri:cut=" and" id="N0x9a175e8.0x98b8888">National Institute of Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</nlm:aff></affiliation></author><author><name sortKey="Duraisingh, Manoj" sort="Duraisingh, Manoj" uniqKey="Duraisingh M" first="Manoj" last="Duraisingh">Manoj Duraisingh</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation></author><author><name sortKey="Mcelwain, Terry F" sort="Mcelwain, Terry F" uniqKey="Mcelwain T" first="Terry F." last="Mcelwain">Terry F. Mcelwain</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Department of Microbiology & Immunology and the Cooperative Research Centre for Vaccine Technology, The University of Melbourne, Victoria 3010, Australia,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA</nlm:aff></affiliation></author><author><name sortKey="Scherf, Artur" sort="Scherf, Artur" uniqKey="Scherf A" first="Artur" last="Scherf">Artur Scherf</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 1960, Institut Pasteur, F-75724 Paris Cedex 15, France,</nlm:aff></affiliation></author><author><name sortKey="Cowman, Alan F" sort="Cowman, Alan F" uniqKey="Cowman A" first="Alan F." last="Cowman">Alan F. Cowman</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation></author><author><name sortKey="Crabb, Brendan S" sort="Crabb, Brendan S" uniqKey="Crabb B" first="Brendan S." last="Crabb">Brendan S. Crabb</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Corresponding author e-mail: crabb@wehi.edu.au</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11867551</idno><idno type="pmc">125903</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125903</idno><idno type="RBID">PMC:125903</idno><idno type="doi">10.1093/emboj/21.5.1231</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000F74</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F74</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of <italic>Plasmodium falciparum</italic> chromosomes</title><author><name sortKey="O Onnell, Rebecca A" sort="O Onnell, Rebecca A" uniqKey="O Onnell R" first="Rebecca A." last="O Onnell">Rebecca A. O Onnell</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Department of Microbiology & Immunology and the Cooperative Research Centre for Vaccine Technology, The University of Melbourne, Victoria 3010, Australia,</nlm:aff></affiliation></author><author><name sortKey="Freitas Junior, Lucio H" sort="Freitas Junior, Lucio H" uniqKey="Freitas Junior L" first="Lúcio H." last="Freitas-Junior">Lúcio H. Freitas-Junior</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 1960, Institut Pasteur, F-75724 Paris Cedex 15, France,</nlm:aff></affiliation></author><author><name sortKey="Preiser, Peter R" sort="Preiser, Peter R" uniqKey="Preiser P" first="Peter R." last="Preiser">Peter R. Preiser</name><affiliation><nlm:aff wicri:cut=" and" id="N0x9a175e8.0x98b8888">National Institute of Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</nlm:aff></affiliation></author><author><name sortKey="Williamson, Donald H" sort="Williamson, Donald H" uniqKey="Williamson D" first="Donald H." last="Williamson">Donald H. Williamson</name><affiliation><nlm:aff wicri:cut=" and" id="N0x9a175e8.0x98b8888">National Institute of Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</nlm:aff></affiliation></author><author><name sortKey="Duraisingh, Manoj" sort="Duraisingh, Manoj" uniqKey="Duraisingh M" first="Manoj" last="Duraisingh">Manoj Duraisingh</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation></author><author><name sortKey="Mcelwain, Terry F" sort="Mcelwain, Terry F" uniqKey="Mcelwain T" first="Terry F." last="Mcelwain">Terry F. Mcelwain</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Department of Microbiology & Immunology and the Cooperative Research Centre for Vaccine Technology, The University of Melbourne, Victoria 3010, Australia,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA</nlm:aff></affiliation></author><author><name sortKey="Scherf, Artur" sort="Scherf, Artur" uniqKey="Scherf A" first="Artur" last="Scherf">Artur Scherf</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 1960, Institut Pasteur, F-75724 Paris Cedex 15, France,</nlm:aff></affiliation></author><author><name sortKey="Cowman, Alan F" sort="Cowman, Alan F" uniqKey="Cowman A" first="Alan F." last="Cowman">Alan F. Cowman</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation></author><author><name sortKey="Crabb, Brendan S" sort="Crabb, Brendan S" uniqKey="Crabb B" first="Brendan S." last="Crabb">Brendan S. Crabb</name><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9a175e8.0x98b8888">Corresponding author e-mail: crabb@wehi.edu.au</nlm:aff></affiliation></author></analytic><series><title level="j">The EMBO Journal</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Bacterial plasmids introduced into the human malaria parasite <italic>Plasmodium falciparum</italic> replicate well but are poorly segregated during mitosis. In this paper, we screened a random <italic>P.falciparum</italic> genomic library in order to identify sequences that overcome this segregation defect. Using this approach, we selected for parasites that harbor a unique 21 bp repeat sequence known as Rep20. Rep20 is one of six different repeats found in the subtelomeric regions of all <italic>P.falciparum</italic> chromosomes but which is not found in other eukaryotes or in other plasmodia. Using a number of approaches, we demonstrate that Rep20 sequences lead to dramatically improved episomal maintenance by promoting plasmid segregation between daughter merozoites. We show that Rep20<sup>+</sup>, but not Rep20<sup>–</sup>, plasmids co-localize with terminal chromosomal clusters, indicating that Rep20 mediates plasmid tethering to chromosomes, a mechanism that explains the improved segregation phenotype. This study implicates a direct role for Rep20 in the physical association of chromosome ends, which is a process that facilitates the generation of diversity in the terminally located <italic>P.falciparum</italic> virulence genes.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">EMBO J</journal-id><journal-title>The EMBO Journal</journal-title><issn pub-type="ppub">0261-4189</issn><issn pub-type="epub">1460-2075</issn><publisher><publisher-name>Oxford University Press</publisher-name><publisher-loc>Oxford, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11867551</article-id><article-id pub-id-type="pmc">125903</article-id><article-id pub-id-type="publisher-id">cdf116</article-id><article-id pub-id-type="doi">10.1093/emboj/21.5.1231</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of <italic>Plasmodium falciparum</italic> chromosomes</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>O’Donnell</surname><given-names>Rebecca A.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">1</xref><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">2</xref></contrib><contrib contrib-type="author"><name><surname>Freitas-Junior</surname><given-names>Lúcio H.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">3</xref></contrib><contrib contrib-type="author"><name><surname>Preiser</surname><given-names>Peter R.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">4</xref></contrib><contrib contrib-type="author"><name><surname>Williamson</surname><given-names>Donald H.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">4</xref></contrib><contrib contrib-type="author"><name><surname>Duraisingh</surname><given-names>Manoj</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">1</xref></contrib><contrib contrib-type="author"><name><surname>McElwain</surname><given-names>Terry F.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">1</xref><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">2</xref><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">5</xref></contrib><contrib contrib-type="author"><name><surname>Scherf</surname><given-names>Artur</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">3</xref></contrib><contrib contrib-type="author"><name><surname>Cowman</surname><given-names>Alan F.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">1</xref></contrib><contrib contrib-type="author"><name><surname>Crabb</surname><given-names>Brendan S.</given-names></name><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">1</xref><xref ref-type="aff" rid="N0x9a175e8.0x98b8888">6</xref></contrib></contrib-group><aff id="N0x9a175e8.0x98b8888"><label>1</label>The Walter & Eliza Hall Institute of Medical Research, Victoria 3050,<label>2</label>Department of Microbiology & Immunology and the Cooperative Research Centre for Vaccine Technology, The University of Melbourne, Victoria 3010, Australia,<label>3</label>Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 1960, Institut Pasteur, F-75724 Paris Cedex 15, France,<label>4</label>National Institute of Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK and<label>5</label>Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA<label>6</label>Corresponding author e-mail: crabb@wehi.edu.au</aff><pub-date pub-type="ppub"><day>1</day><month>3</month><year>2002</year></pub-date><volume>21</volume><issue>5</issue><fpage>1231</fpage><lpage>1239</lpage><ext-link ext-link-type="uri" xlink:href="http://www.emboj.oupjournals.org/content/vol21/issue5/"></ext-link><history><date date-type="received"><day>26</day><month>11</month><year>2001</year></date><date date-type="rev-recd"><day>11</day><month>1</month><year>2002</year></date><date date-type="accepted"><day>11</day><month>1</month><year>2002</year></date></history><copyright-statement>Copyright © 2002 European Molecular Biology Organization</copyright-statement><copyright-year>2002</copyright-year><abstract><p>Bacterial plasmids introduced into the human malaria parasite <italic>Plasmodium falciparum</italic> replicate well but are poorly segregated during mitosis. In this paper, we screened a random <italic>P.falciparum</italic> genomic library in order to identify sequences that overcome this segregation defect. Using this approach, we selected for parasites that harbor a unique 21 bp repeat sequence known as Rep20. Rep20 is one of six different repeats found in the subtelomeric regions of all <italic>P.falciparum</italic> chromosomes but which is not found in other eukaryotes or in other plasmodia. Using a number of approaches, we demonstrate that Rep20 sequences lead to dramatically improved episomal maintenance by promoting plasmid segregation between daughter merozoites. We show that Rep20<sup>+</sup>, but not Rep20<sup>–</sup>, plasmids co-localize with terminal chromosomal clusters, indicating that Rep20 mediates plasmid tethering to chromosomes, a mechanism that explains the improved segregation phenotype. This study implicates a direct role for Rep20 in the physical association of chromosome ends, which is a process that facilitates the generation of diversity in the terminally located <italic>P.falciparum</italic> virulence genes.</p></abstract><kwd-group><kwd>plasmid segregation/<italic>Plasmodium falciparum</italic>/Rep20/terminal chromosomal cluster/transfection</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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