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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core</title><author><name sortKey="Diprose, J M" sort="Diprose, J M" uniqKey="Diprose J" first="J. M." last="Diprose">J. M. Diprose</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation></author><author><name sortKey="Burroughs, J N" sort="Burroughs, J N" uniqKey="Burroughs J" first="J. N." last="Burroughs">J. N. Burroughs</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Institute for Animal Health, Pirbright Laboratories, Ash Road, Pirbright, Woking GU24 0NF,</nlm:aff></affiliation></author><author><name sortKey="Sutton, G C" sort="Sutton, G C" uniqKey="Sutton G" first="G. C." last="Sutton">G. C. Sutton</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation></author><author><name sortKey="Goldsmith, A" sort="Goldsmith, A" uniqKey="Goldsmith A" first="A." last="Goldsmith">A. Goldsmith</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: 12 The Laurels, Tetsworth OX9 7BH, UK</nlm:aff></affiliation></author><author><name sortKey="Gouet, P" sort="Gouet, P" uniqKey="Gouet P" first="P." last="Gouet">P. Gouet</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: CNRS IBCP – Bio-Cristallographie 7, passage du Vercors, 69367 Lyon Cedex 07, France</nlm:aff></affiliation></author><author><name sortKey="Malby, R" sort="Malby, R" uniqKey="Malby R" first="R." last="Malby">R. Malby</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: Biomolecular Research Institute, 343 Royal Parade, Parkville, Victoria 3052, Australia</nlm:aff></affiliation></author><author><name sortKey="Overton, I" sort="Overton, I" uniqKey="Overton I" first="I." last="Overton">I. Overton</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: 141a St James Road, Prescot, Merseyside L34 2RD, UK</nlm:aff></affiliation></author><author><name sortKey="Zientara, S" sort="Zientara, S" uniqKey="Zientara S" first="S." last="Ziéntara">S. Ziéntara</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">CNEVA Alfort, Laboratoire Central de Recherches Vétérinaires, 22 rue Pierre Curie, BP67, 94703 Maisons Alfort Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Mertens, P P C" sort="Mertens, P P C" uniqKey="Mertens P" first="P. P. C." last="Mertens">P. P. C. Mertens</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Institute for Animal Health, Pirbright Laboratories, Ash Road, Pirbright, Woking GU24 0NF,</nlm:aff></affiliation></author><author><name sortKey="Stuart, D I" sort="Stuart, D I" uniqKey="Stuart D" first="D. I." last="Stuart">D. I. Stuart</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=" and" id="N0x9af92a0.0x9cf1b60">Oxford Centre for Molecular Sciences, Oxford University, New Chemistry Building, South Parks Road, Oxford OX1 3QT, UK</nlm:aff></affiliation></author><author><name sortKey="Grimes, J M" sort="Grimes, J M" uniqKey="Grimes J" first="J. M." last="Grimes">J. M. Grimes</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Corresponding author e-mail: jonathan@strubi.ox.ac.uk</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11742999</idno><idno type="pmc">125797</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125797</idno><idno type="RBID">PMC:125797</idno><idno type="doi">10.1093/emboj/20.24.7229</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000F73</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F73</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core</title><author><name sortKey="Diprose, J M" sort="Diprose, J M" uniqKey="Diprose J" first="J. M." last="Diprose">J. M. Diprose</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation></author><author><name sortKey="Burroughs, J N" sort="Burroughs, J N" uniqKey="Burroughs J" first="J. N." last="Burroughs">J. N. Burroughs</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Institute for Animal Health, Pirbright Laboratories, Ash Road, Pirbright, Woking GU24 0NF,</nlm:aff></affiliation></author><author><name sortKey="Sutton, G C" sort="Sutton, G C" uniqKey="Sutton G" first="G. C." last="Sutton">G. C. Sutton</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation></author><author><name sortKey="Goldsmith, A" sort="Goldsmith, A" uniqKey="Goldsmith A" first="A." last="Goldsmith">A. Goldsmith</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: 12 The Laurels, Tetsworth OX9 7BH, UK</nlm:aff></affiliation></author><author><name sortKey="Gouet, P" sort="Gouet, P" uniqKey="Gouet P" first="P." last="Gouet">P. Gouet</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: CNRS IBCP – Bio-Cristallographie 7, passage du Vercors, 69367 Lyon Cedex 07, France</nlm:aff></affiliation></author><author><name sortKey="Malby, R" sort="Malby, R" uniqKey="Malby R" first="R." last="Malby">R. Malby</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: Biomolecular Research Institute, 343 Royal Parade, Parkville, Victoria 3052, Australia</nlm:aff></affiliation></author><author><name sortKey="Overton, I" sort="Overton, I" uniqKey="Overton I" first="I." last="Overton">I. Overton</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Present address: 141a St James Road, Prescot, Merseyside L34 2RD, UK</nlm:aff></affiliation></author><author><name sortKey="Zientara, S" sort="Zientara, S" uniqKey="Zientara S" first="S." last="Ziéntara">S. Ziéntara</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">CNEVA Alfort, Laboratoire Central de Recherches Vétérinaires, 22 rue Pierre Curie, BP67, 94703 Maisons Alfort Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Mertens, P P C" sort="Mertens, P P C" uniqKey="Mertens P" first="P. P. C." last="Mertens">P. P. C. Mertens</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Institute for Animal Health, Pirbright Laboratories, Ash Road, Pirbright, Woking GU24 0NF,</nlm:aff></affiliation></author><author><name sortKey="Stuart, D I" sort="Stuart, D I" uniqKey="Stuart D" first="D. I." last="Stuart">D. I. Stuart</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=" and" id="N0x9af92a0.0x9cf1b60">Oxford Centre for Molecular Sciences, Oxford University, New Chemistry Building, South Parks Road, Oxford OX1 3QT, UK</nlm:aff></affiliation></author><author><name sortKey="Grimes, J M" sort="Grimes, J M" uniqKey="Grimes J" first="J. M." last="Grimes">J. M. Grimes</name><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,</nlm:aff></affiliation><affiliation><nlm:aff id="N0x9af92a0.0x9cf1b60">Corresponding author e-mail: jonathan@strubi.ox.ac.uk</nlm:aff></affiliation></author></analytic><series><title level="j">The EMBO Journal</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg<sup>2+</sup> ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by-products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">EMBO J</journal-id><journal-title>The EMBO Journal</journal-title><issn pub-type="ppub">0261-4189</issn><issn pub-type="epub">1460-2075</issn><publisher><publisher-name>Oxford University Press</publisher-name><publisher-loc>Oxford, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11742999</article-id><article-id pub-id-type="pmc">125797</article-id><article-id pub-id-type="publisher-id">cde709</article-id><article-id pub-id-type="doi">10.1093/emboj/20.24.7229</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Diprose</surname><given-names>J.M.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref></contrib><contrib contrib-type="author"><name><surname>Burroughs</surname><given-names>J.N.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">2</xref></contrib><contrib contrib-type="author"><name><surname>Sutton</surname><given-names>G.C.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref></contrib><contrib contrib-type="author"><name><surname>Goldsmith</surname><given-names>A.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">3</xref></contrib><contrib contrib-type="author"><name><surname>Gouet</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">4</xref></contrib><contrib contrib-type="author"><name><surname>Malby</surname><given-names>R.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">5</xref></contrib><contrib contrib-type="author"><name><surname>Overton</surname><given-names>I.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">6</xref></contrib><contrib contrib-type="author"><name><surname>Ziéntara</surname><given-names>S.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">7</xref></contrib><contrib contrib-type="author"><name><surname>Mertens</surname><given-names>P.P.C.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">2</xref></contrib><contrib contrib-type="author"><name><surname>Stuart</surname><given-names>D.I.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">8</xref></contrib><contrib contrib-type="author"><name><surname>Grimes</surname><given-names>J.M.</given-names></name><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">1</xref><xref ref-type="aff" rid="N0x9af92a0.0x9cf1b60">9</xref></contrib></contrib-group><aff id="N0x9af92a0.0x9cf1b60"><label>1</label>Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Headington, Oxford OX3 7BN,<label>2</label>Institute for Animal Health, Pirbright Laboratories, Ash Road, Pirbright, Woking GU24 0NF,<label>8</label>Oxford Centre for Molecular Sciences, Oxford University, New Chemistry Building, South Parks Road, Oxford OX1 3QT, UK and<label>7</label>CNEVA Alfort, Laboratoire Central de Recherches Vétérinaires, 22 rue Pierre Curie, BP67, 94703 Maisons Alfort Cedex, France<label>3</label>Present address: 12 The Laurels, Tetsworth OX9 7BH, UK<label>4</label>Present address: CNRS IBCP – Bio-Cristallographie 7, passage du Vercors, 69367 Lyon Cedex 07, France<label>5</label>Present address: Biomolecular Research Institute, 343 Royal Parade, Parkville, Victoria 3052, Australia<label>6</label>Present address: 141a St James Road, Prescot, Merseyside L34 2RD, UK<label>9</label>Corresponding author e-mail: jonathan@strubi.ox.ac.uk</aff><pub-date pub-type="ppub"><day>17</day><month>12</month><year>2001</year></pub-date><volume>20</volume><issue>24</issue><fpage>7229</fpage><lpage>7239</lpage><ext-link ext-link-type="uri" xlink:href="http://www.emboj.oupjournals.org/content/vol20/issue24/"></ext-link><history><date date-type="received"><day>22</day><month>8</month><year>2001</year></date><date date-type="rev-recd"><day>25</day><month>10</month><year>2001</year></date><date date-type="accepted"><day>1</day><month>11</month><year>2001</year></date></history><copyright-statement>Copyright © 2001 European Molecular Biology Organization</copyright-statement><copyright-year>2001</copyright-year><abstract><p>The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double-stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X-ray crystallography. Mg<sup>2+</sup> ions produce a slight expansion of the capsid around the 5-fold axes. Oligonucleotide soaks demonstrate that the 5-fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by-products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.</p></abstract><kwd-group><kwd>bluetongue virus/Reoviridae/transcription/virus structure/X-ray crystallography</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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