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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture</title><author><name sortKey="Nichols, Carol Mancuso" sort="Nichols, Carol Mancuso" uniqKey="Nichols C" first="Carol Mancuso" last="Nichols">Carol Mancuso Nichols</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bowman, John P" sort="Bowman, John P" uniqKey="Bowman J" first="John P." last="Bowman">John P. Bowman</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Guezennec, Jean" sort="Guezennec, Jean" uniqKey="Guezennec J" first="Jean" last="Guezennec">Jean Guezennec</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16000756</idno><idno type="pmc">1169062</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1169062</idno><idno type="RBID">PMC:1169062</idno><idno type="doi">10.1128/AEM.71.7.3519-3523.2005</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000F58</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F58</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture</title><author><name sortKey="Nichols, Carol Mancuso" sort="Nichols, Carol Mancuso" uniqKey="Nichols C" first="Carol Mancuso" last="Nichols">Carol Mancuso Nichols</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bowman, John P" sort="Bowman, John P" uniqKey="Bowman J" first="John P." last="Bowman">John P. Bowman</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Guezennec, Jean" sort="Guezennec, Jean" uniqKey="Guezennec J" first="Jean" last="Guezennec">Jean Guezennec</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus <italic>Pseudoalteromonas</italic> and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at −2°C, 10°C, and 20°C, and cell number, optical density, pH, glucose concentration, and viscosity were monitored. The yield of EPS at −2°C and 10°C was 30 times higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature impose pressures on microbial growth and survival. The EPS produced at −2°C and 10°C had a higher uronic acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the Antarctic marine environment may have important ecological implications.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id><journal-id journal-id-type="publisher-id">aem</journal-id><journal-title>Applied and Environmental Microbiology</journal-title><issn pub-type="ppub">0099-2240</issn><issn pub-type="epub">1098-5336</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16000756</article-id><article-id pub-id-type="pmc">1169062</article-id><article-id pub-id-type="publisher-id">2535-04</article-id><article-id pub-id-type="doi">10.1128/AEM.71.7.3519-3523.2005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Microbial Ecology</subject></subj-group></article-categories><title-group><article-title>Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nichols</surname><given-names>Carol Mancuso</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Bowman</surname><given-names>John P.</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Guezennec</surname><given-names>Jean</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib></contrib-group><aff id="aff1">School of Agricultural Science, University of Tasmania, Hobart, Tasmania, Australia,<label>1</label> Australian Food Safety Centre of Excellence, University of Tasmania, Hobart, Tasmania, Australia,<label>2</label> Institute Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, DRV/VP, Plouzané, France<label>3</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Centre for Marine Science and School of Agricultural Science, GPO Box 252-54, University of Tasmania, Hobart 7000, Australia. Phone: 61 3 6226 2620. Fax: 61 3 6226 2642. E-mail: <email>C.A.Mancuso@utas.edu.au</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>7</month><year>2005</year></pub-date><volume>71</volume><issue>7</issue><fpage>3519</fpage><lpage>3523</lpage><history><date date-type="received"><day>18</day><month>11</month><year>2004</year></date><date date-type="accepted"><day>31</day><month>1</month><year>2005</year></date></history><copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement><copyright-year>2005</copyright-year><abstract><p>The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus <italic>Pseudoalteromonas</italic> and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at −2°C, 10°C, and 20°C, and cell number, optical density, pH, glucose concentration, and viscosity were monitored. The yield of EPS at −2°C and 10°C was 30 times higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature impose pressures on microbial growth and survival. The EPS produced at −2°C and 10°C had a higher uronic acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the Antarctic marine environment may have important ecological implications.</p></abstract><custom-meta-wrap><custom-meta><meta-name>PDF filename</meta-name><meta-value>zam00705003519</meta-value></custom-meta></custom-meta-wrap></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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