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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of an <italic>mlrA</italic>
Gene-Directed TaqMan PCR Assay for Quantitative Assessment of Microcystin-Degrading Bacteria within Water Treatment Plant Sand Filter Biofilms<xref ref-type="fn" rid="fn1">▿</xref>
</title>
<author><name sortKey="Hoefel, Daniel" sort="Hoefel, Daniel" uniqKey="Hoefel D" first="Daniel" last="Hoefel">Daniel Hoefel</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Adriansen, Caroline M M" sort="Adriansen, Caroline M M" uniqKey="Adriansen C" first="Caroline M. M." last="Adriansen">Caroline M. M. Adriansen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bouyssou, Magali A C" sort="Bouyssou, Magali A C" uniqKey="Bouyssou M" first="Magali A. C." last="Bouyssou">Magali A. C. Bouyssou</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Saint, Christopher P" sort="Saint, Christopher P" uniqKey="Saint C" first="Christopher P." last="Saint">Christopher P. Saint</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Newcombe, Gayle" sort="Newcombe, Gayle" uniqKey="Newcombe G" first="Gayle" last="Newcombe">Gayle Newcombe</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ho, Lionel" sort="Ho, Lionel" uniqKey="Ho L" first="Lionel" last="Ho">Lionel Ho</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19502429</idno>
<idno type="pmc">2725489</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725489</idno>
<idno type="RBID">PMC:2725489</idno>
<idno type="doi">10.1128/AEM.00036-09</idno>
<date when="2009">2009</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of an <italic>mlrA</italic>
Gene-Directed TaqMan PCR Assay for Quantitative Assessment of Microcystin-Degrading Bacteria within Water Treatment Plant Sand Filter Biofilms<xref ref-type="fn" rid="fn1">▿</xref>
</title>
<author><name sortKey="Hoefel, Daniel" sort="Hoefel, Daniel" uniqKey="Hoefel D" first="Daniel" last="Hoefel">Daniel Hoefel</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Adriansen, Caroline M M" sort="Adriansen, Caroline M M" uniqKey="Adriansen C" first="Caroline M. M." last="Adriansen">Caroline M. M. Adriansen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bouyssou, Magali A C" sort="Bouyssou, Magali A C" uniqKey="Bouyssou M" first="Magali A. C." last="Bouyssou">Magali A. C. Bouyssou</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Saint, Christopher P" sort="Saint, Christopher P" uniqKey="Saint C" first="Christopher P." last="Saint">Christopher P. Saint</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Newcombe, Gayle" sort="Newcombe, Gayle" uniqKey="Newcombe G" first="Gayle" last="Newcombe">Gayle Newcombe</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ho, Lionel" sort="Ho, Lionel" uniqKey="Ho L" first="Lionel" last="Ho">Lionel Ho</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>We report for the first time a quantitative <italic>mlrA</italic>
gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate <italic>mlr</italic>
<italic>A</italic>
gene abundance with microcystin removal efficiency.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">aem</journal-id>
<journal-title>Applied and Environmental Microbiology</journal-title>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology (ASM)</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19502429</article-id>
<article-id pub-id-type="pmc">2725489</article-id>
<article-id pub-id-type="publisher-id">0036-09</article-id>
<article-id pub-id-type="doi">10.1128/AEM.00036-09</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Methods</subject>
</subj-group>
</article-categories>
<title-group><article-title>Development of an <italic>mlrA</italic>
Gene-Directed TaqMan PCR Assay for Quantitative Assessment of Microcystin-Degrading Bacteria within Water Treatment Plant Sand Filter Biofilms<xref ref-type="fn" rid="fn1">▿</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Hoefel</surname>
<given-names>Daniel</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Adriansen</surname>
<given-names>Caroline M. M.</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bouyssou</surname>
<given-names>Magali A. C.</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Saint</surname>
<given-names>Christopher P.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Newcombe</surname>
<given-names>Gayle</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ho</surname>
<given-names>Lionel</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
</contrib-group>
<aff id="aff1">Australian Water Quality Centre, South Australian Water Corporation, 250 Victoria Square, South Australia 5000, Australia,<label>1</label>
School of Earth and Environmental Sciences, University of Adelaide, South Australia 5005, Australia,<label>2</label>
École Nationale Supérieure D'Ingénieurs De Limoges, 16 Rue Atlantis—Parc Ester, B.P. 6804, F87068 Limoges Cedex, France<label>3</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address: Australian Water Quality Centre, South Australian Water Corporation, 250 Victoria Square, Adelaide, South Australia 5000, Australia. Phone: 61874242142. Fax: 61870032142. E-mail: <email>daniel.hoefel@sawater.com.au</email>
</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>8</month>
<year>2009</year>
</pub-date>
<pub-date pub-type="epub"><day>5</day>
<month>6</month>
<year>2009</year>
</pub-date>
<volume>75</volume>
<issue>15</issue>
<fpage>5167</fpage>
<lpage>5169</lpage>
<history><date date-type="received"><day>7</day>
<month>1</month>
<year>2009</year>
</date>
<date date-type="accepted"><day>1</day>
<month>6</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2009, American Society for Microbiology</copyright-statement>
</permissions>
<self-uri xlink:title="pdf" xlink:href="zam01509005167.pdf"></self-uri>
<abstract><p>We report for the first time a quantitative <italic>mlrA</italic>
gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate <italic>mlr</italic>
<italic>A</italic>
gene abundance with microcystin removal efficiency.</p>
</abstract>
</article-meta>
</front>
<floats-wrap><fig position="float" id="f1"><label>FIG. 1.</label>
<caption><p>(a) Abundance of 16S rDNA (closed circles) and <italic>mlrA</italic>
gene copies (closed squares) within the sand filter column biofilm. (b) Abundance of <italic>mlrA</italic>
gene copies (closed squares) within the sand filter column biofilm and the percentage of microcystin-LR removed (open triangles) through the column. Error bars represent standard deviations of the results for triplicate analyses.</p>
</caption>
<graphic xlink:href="zam0150901480001"></graphic>
</fig>
<fig position="float" id="f2"><label>FIG. 2.</label>
<caption><p>Cluster analysis of DGGE band profiles for the sand filter column study. Scale bar represents two band differences.</p>
</caption>
<graphic xlink:href="zam0150901480002"></graphic>
</fig>
</floats-wrap>
</pmc>
</record>
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