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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nickel biopathways in tropical nickel hyperaccumulating trees from Sabah (Malaysia)</title><author><name sortKey="Van Der Ent, Antony" sort="Van Der Ent, Antony" uniqKey="Van Der Ent A" first="Antony" last="Van Der Ent">Antony Van Der Ent</name><affiliation><nlm:aff id="a1"><institution>Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland</institution>, Queensland,<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="a2"><institution>Université de Lorraine–INRA, Laboratoire Sols et Environnement</institution>, UMR 1120,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Callahan, Damien L" sort="Callahan, Damien L" uniqKey="Callahan D" first="Damien L." last="Callahan">Damien L. Callahan</name><affiliation><nlm:aff id="a3"><institution>Deakin University, Geelong, Australia. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology (Burwood Campus)</institution>, Victoria,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Noller, Barry N" sort="Noller, Barry N" uniqKey="Noller B" first="Barry N." last="Noller">Barry N. Noller</name><affiliation><nlm:aff id="a1"><institution>Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland</institution>, Queensland,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Mesjasz Przybylowicz, Jolanta" sort="Mesjasz Przybylowicz, Jolanta" uniqKey="Mesjasz Przybylowicz J" first="Jolanta" last="Mesjasz-Przybylowicz">Jolanta Mesjasz-Przybylowicz</name><affiliation><nlm:aff id="a4"><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></nlm:aff></affiliation></author><author><name sortKey="Przybylowicz, Wojciech J" sort="Przybylowicz, Wojciech J" uniqKey="Przybylowicz W" first="Wojciech J." last="Przybylowicz">Wojciech J. Przybylowicz</name><affiliation><nlm:aff id="a4"><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></nlm:aff></affiliation><affiliation><nlm:aff id="a5"><institution>AGH University of Science and Technology, Faculty of Physics & Applied Computer Science</institution>, Krakow,<country>Poland</country></nlm:aff></affiliation></author><author><name sortKey="Barnabas, Alban" sort="Barnabas, Alban" uniqKey="Barnabas A" first="Alban" last="Barnabas">Alban Barnabas</name><affiliation><nlm:aff id="a4"><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></nlm:aff></affiliation></author><author><name sortKey="Harris, Hugh H" sort="Harris, Hugh H" uniqKey="Harris H" first="Hugh H." last="Harris">Hugh H. Harris</name><affiliation><nlm:aff id="a6"><institution>Department of Chemistry, The University of Adelaide, South Australia</institution>,<country>Australia</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28205587</idno><idno type="pmc">5311975</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311975</idno><idno type="RBID">PMC:5311975</idno><idno type="doi">10.1038/srep41861</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000870</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000870</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nickel biopathways in tropical nickel hyperaccumulating trees from Sabah (Malaysia)</title><author><name sortKey="Van Der Ent, Antony" sort="Van Der Ent, Antony" uniqKey="Van Der Ent A" first="Antony" last="Van Der Ent">Antony Van Der Ent</name><affiliation><nlm:aff id="a1"><institution>Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland</institution>, Queensland,<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="a2"><institution>Université de Lorraine–INRA, Laboratoire Sols et Environnement</institution>, UMR 1120,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Callahan, Damien L" sort="Callahan, Damien L" uniqKey="Callahan D" first="Damien L." last="Callahan">Damien L. Callahan</name><affiliation><nlm:aff id="a3"><institution>Deakin University, Geelong, Australia. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology (Burwood Campus)</institution>, Victoria,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Noller, Barry N" sort="Noller, Barry N" uniqKey="Noller B" first="Barry N." last="Noller">Barry N. Noller</name><affiliation><nlm:aff id="a1"><institution>Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland</institution>, Queensland,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Mesjasz Przybylowicz, Jolanta" sort="Mesjasz Przybylowicz, Jolanta" uniqKey="Mesjasz Przybylowicz J" first="Jolanta" last="Mesjasz-Przybylowicz">Jolanta Mesjasz-Przybylowicz</name><affiliation><nlm:aff id="a4"><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></nlm:aff></affiliation></author><author><name sortKey="Przybylowicz, Wojciech J" sort="Przybylowicz, Wojciech J" uniqKey="Przybylowicz W" first="Wojciech J." last="Przybylowicz">Wojciech J. Przybylowicz</name><affiliation><nlm:aff id="a4"><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></nlm:aff></affiliation><affiliation><nlm:aff id="a5"><institution>AGH University of Science and Technology, Faculty of Physics & Applied Computer Science</institution>, Krakow,<country>Poland</country></nlm:aff></affiliation></author><author><name sortKey="Barnabas, Alban" sort="Barnabas, Alban" uniqKey="Barnabas A" first="Alban" last="Barnabas">Alban Barnabas</name><affiliation><nlm:aff id="a4"><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></nlm:aff></affiliation></author><author><name sortKey="Harris, Hugh H" sort="Harris, Hugh H" uniqKey="Harris H" first="Hugh H." last="Harris">Hugh H. Harris</name><affiliation><nlm:aff id="a6"><institution>Department of Chemistry, The University of Adelaide, South Australia</institution>,<country>Australia</country></nlm:aff></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The extraordinary level of accumulation of nickel (Ni) in hyperaccumulator plants is a consequence of specific metal sequestering and transport mechanisms, and knowledge of these processes is critical for advancing an understanding of transition element metabolic regulation in these plants. The Ni biopathways were elucidated in three plant species, <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> (Phyllanthaceae) and <italic>Rinorea bengalensis</italic> (Violaceae), that occur in Sabah (Malaysia) on the Island of Borneo. This study showed that Ni is mainly concentrated in the phloem in roots and stems (up to 16.9% Ni in phloem sap in <italic>Phyllanthus balgooyi</italic>) in all three species. However, the species differ in their leaves – in <italic>P. balgooyi</italic> the highest Ni concentration is in the phloem, but in <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> in the epidermis and in the spongy mesophyll (<italic>R. bengalensis)</italic>. The chemical speciation of Ni<sup>2+</sup> does not substantially differ between the species nor between the plant tissues and transport fluids, and is unambiguously associated with citrate. This study combines ion microbeam (PIXE and RBS) and metabolomics techniques (GC-MS, LC-MS) with synchrotron methods (XAS) to overcome the drawbacks of the individual techniques to quantitatively determine Ni distribution and Ni<sup>2+</sup> chemical speciation in hyperaccumulator plants.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Jaffre, T" uniqKey="Jaffre T">T. Jaffre</name></author><author><name sortKey="Brooks, R R" uniqKey="Brooks R">R. R. Brooks</name></author><author><name sortKey="Lee, J" uniqKey="Lee J">J. Lee</name></author><author><name sortKey="Reeves, R D" uniqKey="Reeves R">R. D. Reeves</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Ent, A" uniqKey="Van Der Ent A">A. van der Ent</name></author><author><name sortKey="Baker, A J" uniqKey="Baker A">A. J. Baker</name></author><author><name sortKey="Reeves, R D" uniqKey="Reeves R">R. D. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28205587</article-id><article-id pub-id-type="pmc">5311975</article-id><article-id pub-id-type="pii">srep41861</article-id><article-id pub-id-type="doi">10.1038/srep41861</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Nickel biopathways in tropical nickel hyperaccumulating trees from Sabah (Malaysia)</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>van der Ent</surname><given-names>Antony</given-names></name><xref ref-type="corresp" rid="c1">a</xref><xref ref-type="aff" rid="a1">1</xref><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Callahan</surname><given-names>Damien L.</given-names></name><xref ref-type="aff" rid="a3">3</xref></contrib><contrib contrib-type="author"><name><surname>Noller</surname><given-names>Barry N.</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Mesjasz-Przybylowicz</surname><given-names>Jolanta</given-names></name><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>Przybylowicz</surname><given-names>Wojciech J.</given-names></name><xref ref-type="aff" rid="a4">4</xref><xref ref-type="aff" rid="a5">5</xref></contrib><contrib contrib-type="author"><name><surname>Barnabas</surname><given-names>Alban</given-names></name><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>Harris</surname><given-names>Hugh H.</given-names></name><xref ref-type="corresp" rid="c2">b</xref><xref ref-type="aff" rid="a6">6</xref></contrib><aff id="a1"><label>1</label><institution>Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland</institution>, Queensland,<country>Australia</country></aff><aff id="a2"><label>2</label><institution>Université de Lorraine–INRA, Laboratoire Sols et Environnement</institution>, UMR 1120,<country>France</country></aff><aff id="a3"><label>3</label><institution>Deakin University, Geelong, Australia. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology (Burwood Campus)</institution>, Victoria,<country>Australia</country></aff><aff id="a4"><label>4</label><institution>Materials Research Department, iThemba LABS, National Research Foundation, Somerset West</institution>,<country>South Africa</country></aff><aff id="a5"><label>5</label><institution>AGH University of Science and Technology, Faculty of Physics & Applied Computer Science</institution>, Krakow,<country>Poland</country></aff><aff id="a6"><label>6</label><institution>Department of Chemistry, The University of Adelaide, South Australia</institution>,<country>Australia</country></aff></contrib-group><author-notes><corresp id="c1"><label>a</label><email>a.vanderent@uq.edu.au</email></corresp><corresp id="c2"><label>b</label><email>hugh.harris@adelaide.edu.au</email></corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>02</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>7</volume><elocation-id>41861</elocation-id><history><date date-type="received"><day>30</day><month>09</month><year>2016</year></date><date date-type="accepted"><day>04</day><month>01</month><year>2017</year></date></history><permissions><copyright-statement>Copyright © 2017, The Author(s)</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><pmc-comment>author-paid</pmc-comment>
<license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract><p>The extraordinary level of accumulation of nickel (Ni) in hyperaccumulator plants is a consequence of specific metal sequestering and transport mechanisms, and knowledge of these processes is critical for advancing an understanding of transition element metabolic regulation in these plants. The Ni biopathways were elucidated in three plant species, <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> (Phyllanthaceae) and <italic>Rinorea bengalensis</italic> (Violaceae), that occur in Sabah (Malaysia) on the Island of Borneo. This study showed that Ni is mainly concentrated in the phloem in roots and stems (up to 16.9% Ni in phloem sap in <italic>Phyllanthus balgooyi</italic>) in all three species. However, the species differ in their leaves – in <italic>P. balgooyi</italic> the highest Ni concentration is in the phloem, but in <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> in the epidermis and in the spongy mesophyll (<italic>R. bengalensis)</italic>. The chemical speciation of Ni<sup>2+</sup> does not substantially differ between the species nor between the plant tissues and transport fluids, and is unambiguously associated with citrate. This study combines ion microbeam (PIXE and RBS) and metabolomics techniques (GC-MS, LC-MS) with synchrotron methods (XAS) to overcome the drawbacks of the individual techniques to quantitatively determine Ni distribution and Ni<sup>2+</sup> chemical speciation in hyperaccumulator plants.</p></abstract></article-meta></front><body><p>Hyperaccumulators are rare plants that accumulate trace elements to extraordinarily high concentrations in their living aboveground biomass<xref ref-type="bibr" rid="b1">1</xref>. As of 2016 approximately 450 Ni hyperaccumulators have been documented<xref ref-type="bibr" rid="b2">2</xref>, mostly in Cuba (130)<xref ref-type="bibr" rid="b3">3</xref>, Southern Europe and Minor Asia (80–90)<xref ref-type="bibr" rid="b4">4</xref><xref ref-type="bibr" rid="b5">5</xref>, New Caledonia (65)<xref ref-type="bibr" rid="b6">6</xref> and Malaysia (24)<xref ref-type="bibr" rid="b7">7</xref>. The extreme levels of accumulation of trace elements in hyperaccumulator plants are a consequence of specific metal sequestering and transport mechanisms, and knowledge of these processes is critical in understanding the hyperaccumulation phenomenon. Hyperaccumulator plants are therefore an attractive model for understanding transition element metabolic regulation in plants, because of the extreme expression of this phenotype in such plants. Hyperaccumulator plants also hold much promise for potential utilization in phytomining/agromining. This emerging technology is an environmentally sustainable ‘green’ technology that can extract metals such as Ni from soils with significantly reduced environmental impact compared to conventional mining operations<xref ref-type="bibr" rid="b8">8</xref><xref ref-type="bibr" rid="b9">9</xref>. In a phytomining/agromining operation, hyperaccumulator plants (‘metal crops’) are grown on sub-economic ores or waste tailings followed by harvesting and incineration of the biomass to generate a commercial high-grade metal bio-ore<xref ref-type="bibr" rid="b10">10</xref><xref ref-type="bibr" rid="b11">11</xref><xref ref-type="bibr" rid="b12">12</xref>. Nickel phytomining is envisaged to become transformative in the rehabilitation of tropical laterite mining operations as part of the rehabilitation strategies<xref ref-type="bibr" rid="b13">13</xref>.</p><p>Globally the greatest potential for Ni phytomining lies in Indonesia, the Philippines and New Caledonia where extensive ultramafic soils and mining operations exist<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b14">14</xref>. This region has numerous woody hyperaccumulator species that are potentially suitable as ‘metal crops’. Three plant species with particularly high potential are <italic>Phyllanthus balgooyi</italic> Petra Hoffm. & A. J. M. Baker, <italic>Phyllanthus securinegioides</italic> Merr. (Phyllanthaceae) and <italic>Rinorea bengalensis</italic> (Wall.) Kuntze (Violaceae) from Borneo Island<xref ref-type="bibr" rid="b7">7</xref><xref ref-type="bibr" rid="b15">15</xref>. <italic>Phyllanthus</italic> spp. are scandent shrubs attaining up to 9 m in height, whereas <italic>R. bengalensis</italic> is a tree that grows up to 25 m in height. <italic>Phyllanthus balgooyi</italic> (Phyllanthaceae) was only recently discovered and described<xref ref-type="bibr" rid="b16">16</xref> from the Philippines and Malaysian Borneo. This species is highly unusual because its stem exudes a green sap that contains up to 16.9% Ni<xref ref-type="bibr" rid="b17">17</xref><xref ref-type="bibr" rid="b18">18</xref>. Such plant metal concentrations are only rivalled by the New Caledonian tree <italic>Pycnandra acuminata</italic> (Sapotaceae) which has a latex that can contain up to 25.7% Ni<xref ref-type="bibr" rid="b1">1</xref>. <italic>Phyllanthus securinegioides</italic> is also a strong hyperaccumulator, its leaves can contain up to 2.3% Ni and has a green phloem, but does not produce copious amounts of Ni-rich exudate as <italic>P. balgooyi</italic><xref ref-type="bibr" rid="b18">18</xref>. Finally, <italic>R. bengalensis</italic> also has a green phloem in the trunk which contains up to 5% Ni, whereas the leaves can contain 2.7% Ni<xref ref-type="bibr" rid="b17">17</xref>.</p><p>Studies on the spatial elemental distribution in Ni hyperaccumulator plants have largely focussed on a very limited number of mainly small herbaceous species from the families Asteraceae and Brassicaceae (in the genera <italic>Alyssum, Berkheya, Noccaea, Senecio</italic>)<xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b20">20</xref><xref ref-type="bibr" rid="b21">21</xref><xref ref-type="bibr" rid="b22">22</xref><xref ref-type="bibr" rid="b23">23</xref><xref ref-type="bibr" rid="b24">24</xref><xref ref-type="bibr" rid="b25">25</xref><xref ref-type="bibr" rid="b26">26</xref><xref ref-type="bibr" rid="b27">27</xref><xref ref-type="bibr" rid="b28">28</xref><xref ref-type="bibr" rid="b29">29</xref><xref ref-type="bibr" rid="b30">30</xref> with the exception of <italic>Hybanthus floribundus</italic> (Violaceae), which is a small shrub<xref ref-type="bibr" rid="b31">31</xref><xref ref-type="bibr" rid="b32">32</xref> and the tree <italic>Phyllanthus balgooyi</italic><xref ref-type="bibr" rid="b18">18</xref>. Leaves were the most often analysed organs<xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b21">21</xref><xref ref-type="bibr" rid="b22">22</xref><xref ref-type="bibr" rid="b23">23</xref><xref ref-type="bibr" rid="b24">24</xref><xref ref-type="bibr" rid="b25">25</xref><xref ref-type="bibr" rid="b28">28</xref><xref ref-type="bibr" rid="b33">33</xref><xref ref-type="bibr" rid="b34">34</xref>, while stems<xref ref-type="bibr" rid="b18">18</xref><xref ref-type="bibr" rid="b32">32</xref><xref ref-type="bibr" rid="b35">35</xref><xref ref-type="bibr" rid="b36">36</xref><xref ref-type="bibr" rid="b37">37</xref> and roots<xref ref-type="bibr" rid="b21">21</xref><xref ref-type="bibr" rid="b24">24</xref><xref ref-type="bibr" rid="b30">30</xref><xref ref-type="bibr" rid="b36">36</xref><xref ref-type="bibr" rid="b38">38</xref><xref ref-type="bibr" rid="b39">39</xref> were seldom measured. In most hyperaccumulator plants Ni is distributed in all tissues but the highest amount is preferentially accumulated in the foliar epidermal cells<xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b27">27</xref><xref ref-type="bibr" rid="b32">32</xref><xref ref-type="bibr" rid="b34">34</xref><xref ref-type="bibr" rid="b35">35</xref><xref ref-type="bibr" rid="b36">36</xref><xref ref-type="bibr" rid="b37">37</xref><xref ref-type="bibr" rid="b40">40</xref>. The leaf epidermal vacuoles are the main storage sites of Ni in <italic>Alyssum lesbiacum, A. bertolonii, Noccaea (Thlaspi) goesingense</italic><xref ref-type="bibr" rid="b19">19</xref>, <italic>H. floribundus</italic><xref ref-type="bibr" rid="b31">31</xref>, <italic>Alyssum murale</italic><xref ref-type="bibr" rid="b34">34</xref> and <italic>Senecio coronatus</italic><xref ref-type="bibr" rid="b41">41</xref>. An exception to this rule is <italic>Berkheya coddii</italic> where Ni is accumulated mainly in the mesophyll and in the leaf veins/vascular bundles of the leaf<xref ref-type="bibr" rid="b25">25</xref><xref ref-type="bibr" rid="b28">28</xref><xref ref-type="bibr" rid="b29">29</xref><xref ref-type="bibr" rid="b42">42</xref>, while the concentrations in the epidermis are lower than the average value for the whole leaf<xref ref-type="bibr" rid="b43">43</xref>.</p><p>In an earlier nuclear microprobe study the highly distinctive spatial distribution of Ni in <italic>P. balgooyi</italic> was reported with extreme levels of accumulation in the vascular bundles of the leaves and phloem tissues in petioles and stems<xref ref-type="bibr" rid="b18">18</xref>. We expect this type of Ni distribution to be shared with <italic>P. securinegioides</italic> and <italic>R. bengalensis,</italic> on account of the Ni-rich green phloem tissues in all three species.</p><p>A combination of Ni<sup>2+</sup> ligand complexation and compartmentalization at the tissue and cellular-level is involved with tolerance mechanisms in Ni hyperaccumulator plants<xref ref-type="bibr" rid="b22">22</xref><xref ref-type="bibr" rid="b44">44</xref>. Ligand complexation reduces Ni<sup>2+</sup> free ion activity and hence cellular-level toxicity<xref ref-type="bibr" rid="b45">45</xref>. Relevant potential Ni<sup>2+</sup> ligands include O-donors (carboxylic acids), S-donors (metallothioneins and phytochelatins), and N-donors (histidine, nicotianamine)<xref ref-type="bibr" rid="b46">46</xref>. In most hyperaccumulator plants Ni<sup>2+</sup> ligand complexation has been univocally associated with carboxylic acids like citrate and malate<xref ref-type="bibr" rid="b44">44</xref><xref ref-type="bibr" rid="b47">47</xref><xref ref-type="bibr" rid="b48">48</xref>. Elucidating Ni<sup>2+</sup> chemical speciation in hyperaccumulator plants has used chromatography methods (GC-MS, LC-MS) and synchrotron X-ray absorption spectroscopy (XAS) methods<xref ref-type="bibr" rid="b44">44</xref><xref ref-type="bibr" rid="b48">48</xref>. Identification of Ni<sup>2+</sup> ligand complexes in plant tissues with chromatographic methods is limited by the need to physically destroy and extract compounds from plant tissue samples, potentially leading to alteration of Ni<sup>2+</sup> coordination complexes in the extract. In addition, identification of Ni<sup>2+</sup> complexes by electrospray ionisation LC-MS is problematic as complexes can be formed during ionisation, which may not reflect the <italic>in situ</italic> form of Ni<sup>2+</sup>. Synchrotron XAS has the advantage of being able to directly determine Ni<sup>2+</sup> speciation <italic>in situ</italic> in intact frozen-hydrated plant tissues. However, XAS alone is not able to accurately distinguish between different carboxylic acid Ni<sup>2+</sup> complexes that are implicated in hyperaccumulation because these complexes provide very little variation in structure to the EXAFS spectra<xref ref-type="bibr" rid="b22">22</xref>. It is also not possible to determine quantitatively mixed Ni<sup>2+</sup> complex environments with XAS. Therefore, there is a need to combine quantitative molecular techniques such as GC/LC-MS with the unique ability of XAS to determine <italic>in situ</italic> chemical Ni<sup>2+</sup> speciation.</p><p>This study combines nuclear microprobe (micro-Particle-Induced X-Ray Emission – micro-PIXE and micro-proton backscattering spectrometry – micro-BS) and metabolomics techniques (GC-MS, LC-MS) with synchrotron methods (XAS) to overcome the drawbacks of the individual techniques to quantitatively determine spatial distribution and Ni<sup>2+</sup> chemical speciation in leaves, stems and roots of hyperaccumulating plants. As such we aim to reveal elemental distribution and Ni<sup>2+</sup> chemical speciation in the tropical woody hyperaccumulator plants <italic>P. balgooyi, P. securinegioides</italic> and <italic>R. bengalensis</italic>. Specifically, we intend to clarify whether these species have similar patterns of Ni storage and whether Ni<sup>2+</sup> chemical speciation differs between the species and between plant organs and transport liquids.</p><sec disp-level="1"><title>Results</title><sec disp-level="2"><title>Ecology of the hyperaccumulating plants</title><p>All three species are native to per-humid equatorial rainforest. <xref ref-type="fig" rid="f1">Figure 1</xref> shows <italic>P. balgooyi, P. securinegioides</italic> and <italic>R. bengalensis</italic> in the native habitat in Sabah (Malaysia) on the Island of Borneo. The soils on which they grow are derived from serpentinised ultramafic bedrock. Concentrations of Ni, Co and Mn are strongly enriched during soil formation. The mean soil pH is rather similar in the rhizosphere from all three species and circum-neutral (pH 6.4–7.1). Although total Ca and Mg concentrations vary, the exchangeable concentrations are similar between the rhizospheres. The soils are K-deficient with mean exchangeable K<sup>+</sup> ranging from 57–120 μg g<sup>−1</sup>. The mean total Ni concentrations are high (2480–3110 μg g<sup>−1</sup>), but potentially plant available concentrations are moderate with 88–220 μg g<sup>−1</sup> mean DTPA Ni<sup>2+</sup> (<xref ref-type="table" rid="t1">Table 1</xref>).</p></sec><sec disp-level="2"><title>Elemental concentrations and distribution in hyperaccumulator plants</title><p>Bulk analysis by ICP-AES showed that in the three species foliar and tissue concentrations of most elements, except Ni, are mostly unremarkable. However, foliar K concentrations are high (mean 860–8740 μg g<sup>−1</sup>) considering the K-deficient nature of the soil (<xref ref-type="table" rid="t2">Table 2</xref>). In comparison with the soil Ca concentrations (mean 1330–1600 μg g<sup>−1</sup> exchangeable Ca), foliar Ca-concentrations (mean 4290–8020 μg g<sup>−1</sup>) are strongly enriched, especially in <italic>R. bengalensis</italic> (<xref ref-type="table" rid="t2">Table 2</xref>). The high Ni concentrations (mean 5140–7630 μg g<sup>−1</sup>) in the branches, compared to the wood, can be attributed to the presence of phloem tissue, which is extremely high in Ni (mean 8940–65410 μg g<sup>−1</sup>). The phloem sap of <italic>P. balgooyi</italic> is one of the most unusual biological liquids, it contains 12.6–16.9% Ni, and is also enriched in Co (560–2340 μg g<sup>−1</sup>) and Zn (1900–3690 μg g<sup>−1</sup>), but low in major cations such as Ca (660–1960 μg g<sup>−1</sup>) and K (520–1530 μg g<sup>−1</sup>) (<xref ref-type="table" rid="t3">Table 3</xref>).</p><p>Micro-PIXE analysis showed that the most important feature of Ni distribution in roots and stems of all three hyperaccumulators is the exceptional enrichment in the phloem (<xref ref-type="table" rid="t4">Tables 4</xref> and <xref ref-type="table" rid="t5">5</xref>, <xref ref-type="fig" rid="f2">Figs 2</xref> and <xref ref-type="fig" rid="f3">3</xref>). However, the species differ in the leaves – in <italic>P. balgooyi</italic> the highest Ni concentration is in phloem, but in <italic>P. securinegioides</italic> in the epidermis. <italic>Rinorea bengalensis</italic> has the highest Ni concentration in the spongy mesophyll but also high enrichment in the epidermis (<xref ref-type="table" rid="t6">Table 6</xref>, <xref ref-type="fig" rid="f4">Fig. 4</xref>). There are also some other differences. In the roots Ni concentrations in the phloem vary from slightly less than 0.2 wt% in <italic>P. balgooyi</italic> to ca. 0.9 wt% in <italic>P. securinegioides</italic> and 5.6 wt% in <italic>R. bengalensis</italic> (<xref ref-type="table" rid="t4">Table 4</xref>, <xref ref-type="fig" rid="f2">Fig. 2</xref>). In <italic>P. balgooyi</italic> there is also some enrichment in the epidermis, but the concentrations there are significantly lower than in the phloem (180 μg g<sup>−1</sup>). The lowest amounts of Ni were measured in cortex and xylem. In <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> the cortex contains more Ni than the epidermis and xylem (<xref ref-type="table" rid="t4">Table 4</xref>, <xref ref-type="fig" rid="f2">Fig. 2</xref>). Phosphorus, S, K, Ca, and Zn show somewhat similar distribution pattern as Ni, with the highest amounts in the phloem in <italic>P. balgooyi</italic> and <italic>P. securinegioides.</italic> Chlorine is the only element with the highest enrichment in the cortex of <italic>P. balgooyi</italic> but in <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> the highest concentrations are in the phloem (<xref ref-type="table" rid="t4">Table 4</xref>, <xref ref-type="supplementary-material" rid="S1">Supplementary Figures 3, 4</xref> and <xref ref-type="supplementary-material" rid="S1">5</xref>). In <italic>R. bengalensis</italic> P and S show markedly different distribution compared to <italic>P. securinegioides</italic> and <italic>P. balgooyi</italic>, forming two enrichment rings in the cortex and xylem, separated by depletion zone in the phloem (<xref ref-type="table" rid="t4">Table 4</xref>, <xref ref-type="supplementary-material" rid="S1">Supplementary Figures 3, 4</xref> and <xref ref-type="supplementary-material" rid="S1">5</xref>). The highest concentrations of Cl, K, Ca, Cu, Zn, Rb and Sr were found in the phloem. In all three species Si, Ti, Cr, Mn, Fe and Co reach the highest concentrations in the root epidermis.</p><p>The elemental distribution in the stems, petioles and leaves of <italic>P. balgooyi</italic> were reported and discussed earlier<xref ref-type="bibr" rid="b18">18</xref>. The main feature in the stems is the extremely high Ni concentration in the phloem, followed by significant enrichment in the cortex (<xref ref-type="table" rid="t5">Table 5</xref>, <xref ref-type="fig" rid="f3">Fig. 3</xref>). The distributions of Fe, Co, and Zn mirror that of Ni, but their concentrations are much lower. Although in the stems of <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> Ni also shows the highest concentrations in the phloem, some important differences were found. The xylem in <italic>P. securinegioides</italic> can be subdivided into younger and older parts, with the younger part significantly enriched in Ni in comparison with the older parts (500 μg g<sup>−1</sup> and 140 μg g<sup>−1</sup>, respectively) and a very distinct, highly Ni-enriched (up to 2800 μg g<sup>−1</sup>) compressed pith (<xref ref-type="table" rid="t5">Table 5</xref>). In the stems of <italic>R. bengalensis</italic> the highest Ni concentrations are in the phloem, but equally high values were found in the meristematic cortical cells (<xref ref-type="table" rid="t5">Table 5</xref>, <xref ref-type="fig" rid="f3">Fig. 3</xref>), of the order of 0.6 wt% in both cases. The remaining parts of cortex also show Ni enrichment (0.3 wt%) as well as the pith (0.28 wt%).</p><p>In <italic>P. securinegioides</italic> Ca shows a similar distribution pattern compared to <italic>P. balgooyi</italic> with the highest concentration in the phloem, In <italic>R. bengalensis</italic> it also shows enrichment in the phloem, but the highest concentrations are in the epidermis (<xref ref-type="fig" rid="f3">Fig. 3</xref>, <xref ref-type="table" rid="t5">Table 5</xref>). Many small “dots” with high Ca amounts are visible in the pith of this species. In <italic>P. securinegioides</italic> K and S are also concentrated in the phloem area, but their distribution pattern is more “diluted” than that of Ni or Ca, and the highest concentration of K is rather in the vascular cambium region. In <italic>R. bengalensis</italic> the distribution of K shows similarities to that of Ni, but the highest concentration of this element is in the cortex.</p><p>The distribution pattern of S in <italic>R. bengalensis</italic> is somewhat similar to that of Ni, with the highest concentrations in the phloem and cortex. In <italic>P. securinegioides</italic> P is another element forming an enrichment “ring” in the cambium region (<xref ref-type="table" rid="t5">Table 5</xref>, <xref ref-type="supplementary-material" rid="S1">Supplementary Figure 6</xref>). In <italic>R. bengalensis</italic> this element has a very different distribution pattern, with the highest concentrations in the pith; xylem is the second region of its enrichment, although less pronounced (<xref ref-type="table" rid="t5">Table 5</xref>, <xref ref-type="supplementary-material" rid="S1">Supplementary Figure 6</xref>). Silicon, Ti, Cr, Mn, Fe and Co are only enriched in the epidermal area of stems, both in <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic>. Cl is concentrated in the phloem, but it is not evenly distributed in this region (<xref ref-type="supplementary-material" rid="S1">Supplementary Figure 6</xref>). In <italic>P. securinegioides</italic> it forms an enrichment ring in the outer phloem and inner cortex, but in <italic>R. bengalensis</italic> such a ring is visible in the innermost part of phloem.</p><p>In comparison with the leaves of <italic>P. balgooyi</italic>, Ni concentrations in the epidermal in <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> are much higher than in the phloem (<xref ref-type="table" rid="t6">Table 6</xref>, <xref ref-type="fig" rid="f4">Fig. 4</xref>), although there is some enrichment in the phloem, relative to the adjoining xylem in <italic>P. securinegioides</italic>. In <italic>P. securinegioides</italic> the upper epidermis is notably richer in Ni (up to 4 wt%, on average) than the lower epidermis with the adjoining part of spongy mesophyll (on average 1.9 wt%). In <italic>R. bengalensis</italic> the highest concentrations are in the spongy mesophyll and the adjoining lower epidermis. The upper epidermis has slightly lower concentrations, as well as palisade mesophyll. Among elements showing enrichment in epidermal regions, Mn in <italic>P. securinegioides</italic> is only concentrated in the upper epidermis, while in <italic>R. bengalensis</italic> such enrichment extends to the adjoining palisade mesophyll. Iron enrichment in the epidermal regions is much more “patchy” and less pronounced. Calcium in <italic>P. securinegioides</italic> shows the highest enrichment in the phloem, but in both species it is also concentrated in the mesophyll showing a “grainy” inhomogeneous distribution. The distributions of P and Zn are shown in the <xref ref-type="supplementary-material" rid="S1">Supplementary Figure 7</xref>.</p></sec><sec disp-level="2"><title>Chemical speciation of Ni<sup>2+</sup> in hyperaccumulator plants</title><p>X-ray Absorption spectroscopy (XAS) was used to investigate the <italic>in situ</italic> Ni speciation in various tissues across the three plant species. The Ni K-edge XANES spectra of the tissues are presented in <xref ref-type="fig" rid="f5">Fig. 5</xref> (traces <bold>C</bold>-<bold>P</bold>) and visual inspection reveals that the spectra vary little beyond variations in noise level that arise as a result of the varying Ni concentration in the respective tissues. A principal component analysis (PCA) of these fourteen plant spectra suggested that between two and four significant components were present, while visualisation of the PCA eigenvectors (not shown) indicated that the variation was limited to <8350 eV.</p><p>The plant XANES spectra were compared against a number of aqueous solution model complexes of Ni and physiological relevant ligands as well as two insoluble Ni compounds (<xref ref-type="fig" rid="f6">Fig. 6</xref>). Several of the model spectra were easily distinguished from the plant spectra by visual inspection, including complexes with N- or S-donor ligands (<xref ref-type="fig" rid="f6">Fig. 6</xref> - trace <bold>H</bold>), the solids (trace <bold>G</bold>) as well as the complexes with nicotianamine, oxalate or phytate (trace <bold>F</bold>). The remaining spectra of complexes dominated by carboxylate binding matched the plant-based spectra far more closely, but could be differentiated by features centred at either ~8361 and 8400 eV. The lower energy feature varied in intensity between the different models, being more intense and well resolved in the citrate (1:10 Ni:citrate–trace <bold>B</bold>), tartrate and malonate spectra (trace <bold>D</bold>), but less so in the remaining spectra including the plant-based example in <bold>A</bold>.</p><p>Variation in the higher energy feature was more subtle, being either: broad and rounded (1:10 Ni:citrate–trace <bold>B</bold>); narrower and rounded (malate and tartrate in <bold>D</bold>); split and resolved symmetrically (the four traces in <bold>C</bold>); split and resolved asymmetrically (acetylacetonate and malonate in trace <bold>E</bold>); or broad and unresolved (the plant and 1:1 Ni:citrate model in trace <bold>A</bold>).</p><p>Meanwhile, the spectrum of hexaquo Ni<sup>2+</sup> showed intermediate intensity of the peak at 8361 eV and a broad unresolved feature centred on 8400 eV. While this region was very similar to the plant-based spectra, the white line peak (~8349 eV) intensity for the hexaquo complex was significantly greater than in either the plant or carboxylate model spectra, as well as appearing at slightly higher energy as previously reported by others<xref ref-type="bibr" rid="b44">44</xref>.</p><p>In summary, the plant XANES spectra were well matched by the spectrum of the 1:1 solution of Ni(II) and citrate at pH 5.5, and could be distinguished from the spectra of all other model complex spectra by a combination of features at the white line, 8361 and ~8400 eV. The XANES spectra for pH adjusted and unadjusted Ni:citrate were consistent.</p><p>We did attempt a least-squares fitting of the plant spectra with a selection of the model complex XANES spectra but abandoned this approach for several reasons. As noted above, a principal component analysis was not conclusive regarding the number of model compounds that should be included in the linear regression and fit results generated with different numbers of components varied markedly. In addition, and perhaps most importantly, the spectrum of the 1:1 Ni:citrate solution was recorded at a different beamline (the AS XAS beamline; as the ANBF was permanently decommissioned shortly after the spectra of the plants and other models were recorded) than the spectra of the plant tissues. Because the spectral resolution of the two beamlines was not necessarily identical (also potentially affecting energy calibration) the spectrum of the 1:1 citrate model could not be rigorously included in the statistical analyses, despite being apparently the best match by eye.</p><p>We also compared the extended X-ray absorption structure (EXAFS) data of the plant tissues (<xref ref-type="supplementary-material" rid="S1">Supplementary Figure 1</xref> - all recorded at ANBF) with the EXAFS data from a selection of the most closely matched model spectra (<xref ref-type="supplementary-material" rid="S1">Supplementary Figure 2</xref> - recorded at the AS) according to the XANES results. We note that the EXAFS data will be less sensitive to differences in the spectral resolution of the two beamlines. Visual inspection of the k-space data to 10 Å<sup>−1</sup> in <xref ref-type="supplementary-material" rid="S1">Supplementary Figure 1</xref> reveals that the EXAFS for these samples are essentially identical with minor variations noted between 3–4 and 5–6 Å<sup>−1</sup>, particularly for the leaf samples (black traces <bold>F</bold> and <bold>I</bold>). More significant variations were noted in these ranges for the model complexes EXAFS data shown in <xref ref-type="supplementary-material" rid="S1">Supplementary Figure 2</xref> with those EXAFS and variations similar to those previously reported by others<xref ref-type="bibr" rid="b22">22</xref><xref ref-type="bibr" rid="b44">44</xref>. Only the EXAFS of the malonate complex was clearly distinguishable from that of the plant samples (due to a strong oscillation in the EXAFS at ~3.5 Å<sup>−1</sup>) but the EXAFS of the plants was closest to that of the 1:1 citrate model (asymmetrical oscillation between 3–4 Å<sup>−1</sup> and a weak “shoulder” on the low-k side of the oscillation between 5–6 Å<sup>−1</sup>). Again, attempts to fit the plant sample EXAFS with a linear combination of the model complex EXAFS was abandoned because variation in the number of fitted models dramatically altered the fit results.</p><p>Taken together, the XANES and EXAFS data of the plants indicates that the predominant species present in the tissues is a 1:1 Ni complex with citrate and the remainder of the coordination sphere consisting of water ligands. The minor variations in the spectra in the leaf tissues can be explained by the presence of a 1:2 complex of Ni with citrate, producing a slightly more intense peak in the XANES at 8361 eV (<xref ref-type="fig" rid="f5">Fig. 5</xref>–traces <bold>P</bold> and <bold>K</bold>) and, somewhat less clearly, a variation in the EXAFS between 5–6 Å<sup>−1</sup>. It is certainly feasible that the relative proportion of these two species may vary between plant species and between particular tissues in those species. We believe that the more complicated mixtures of ligands identified by other groups in other Ni hyperaccumulators, including malate complexes and others, may be as a result of the fact that they did not include the 1:1 Ni:citrate complex spectrum in their fitting, and the evidence for the presence of these other complexes based purely on XAS data is weak.</p><p>Gas-chromatography (GC-MS) was used for untargeted metabolite profiling in the freeze-dried plant tissues and phloem sap samples. Relative response ratios (RRR’s) were calculated using the metabolite peak area divided by the internal standard area and sample mass. This resulted in a list of 95 compounds, of which mass spectral libraries positively identified 60 compounds (<xref ref-type="table" rid="t7">Table 7</xref>), however the method cannot detect any Ni-complexes. GC-MS was also used to quantify the six most abundant metabolites determined in the metabolomic profiles; these were malate, citrate, fructose, glucose, sucrose and catechin in the <italic>P. balgooyi</italic> phloem sap (<xref ref-type="table" rid="t8">Table 8</xref>). The sugars are of interest, as ref. <xref ref-type="bibr" rid="b48">48</xref> reported the formation of Ni:citrate:sucrose complexes. Ion chromatography (IC) was then used to quantify anions in the <italic>P. balgooyi</italic> phloem sap and as such compile a mass balance of ligands and anions available to complex Ni<sup>2+</sup> (<xref ref-type="table" rid="t9">Table 9</xref>). The pH of the thawed phloem sap was pH 6.54, which aligned well with spot tests taken in the field on fresh sap (~pH 6.0–6.5). The use of LC-MS enabled the identification Ni<sup>2+</sup> containing complexes. A large number of ions containing the isotope pattern of Ni<sup>2+</sup> were present in the chromatograms of the extracts of the plant species. <xref ref-type="fig" rid="f7">Figure 7</xref> shows the mass spectrum of Ni-citrate [M.L<sub>2</sub>-H]<sup>+</sup> from <italic>P. balgooyi</italic> phloem sap (black trace) and theoretical isotope pattern for Ni-citrate (red boxes). The most prominent Ni<sup>2+</sup> containing ion detected in all samples was Ni<sup>2+</sup> citrate [M.L<sub>2</sub>-H]<sup>+</sup>.</p><p>The mass balance presented in <xref ref-type="table" rid="t9">Table 9</xref> shows that the mean anion concentration is 0.46 wt% versus a mean of 76.4 wt% citrate to balance a mean of 15.6 wt% Ni. Taking into account relevant ionic charges, the quantities of citrate together with the relatively minor concentrations of cations are sufficient to complex and ionically balance virtually all of Ni<sup>2+</sup> present in the phloem sap of <italic>P. balgooyi</italic>.</p></sec></sec><sec disp-level="1"><title>Discussion</title><p>All three species have a highly distinctive tissue Ni distribution patterns with extreme levels of accumulation in the phloem of the root and stem. The phloem tissues in the main stem of all three species are green due to the exceptionally high concentration of Ni<sup>2+</sup> ions (<xref ref-type="fig" rid="f1">Fig. 1</xref>) and appear to act as a ‘sink’ with Ni concentrations reaching up to 2.1% in <italic>R. bengalensis</italic> and up to 16.9 wt%, in the phloem sap from <italic>P. balgooyi</italic> (<xref ref-type="table" rid="t2">Table 2</xref>). The difference occurs in leaves – in <italic>P. balgooyi</italic> the highest Ni concentration is in the phloem, but in <italic>P. securinegioides</italic> and <italic>R. bengalensis</italic> in the epidermis and in the spongy mesophyll (<italic>R. bengalensis)</italic>. Nickel accumulation in the leaf epidermal cells of Ni hyperaccumulators appears to be a global trait, with the exception of <italic>B. coddii</italic> and now <italic>P. balgooyi</italic>. Although high accumulation in the vascular bundles is not an anomaly among herbaceous hyperaccumulating plants, most previous investigations concentrated on leaf epidermal tissue as important storage site and did not pay much attention to Ni transport and concentration in the vascular bundles. High concentrations of Ni in the phloem tissue in roots and stems are not only a feature of woody tropical hyperaccumulator plants; significant Ni enrichment in the phloem was reported from the stem of <italic>Senecio coronatus</italic><xref ref-type="bibr" rid="b26">26</xref>, <italic>Alyssum murale</italic><xref ref-type="bibr" rid="b21">21</xref> and in bark/phloem boundary in <italic>A. lesbiacum</italic> and <italic>A. bertolonii</italic><xref ref-type="bibr" rid="b19">19</xref>. High Ni concentrations were also found in the phloem tissues of <italic>Senecio coronatus</italic> roots<xref ref-type="bibr" rid="b30">30</xref>, vascular cylinders of young <italic>Alyssum murale</italic> roots<xref ref-type="bibr" rid="b21">21</xref><xref ref-type="bibr" rid="b24">24</xref> and root vascular tissues of <italic>Berkheya coddii</italic><xref ref-type="bibr" rid="b39">39</xref>. High Ni concentrations in vascular bundles were also measured in the leaves of <italic>B. coddii</italic><xref ref-type="bibr" rid="b29">29</xref><xref ref-type="bibr" rid="b43">43</xref>, <italic>Stackhousia tryonii</italic><xref ref-type="bibr" rid="b37">37</xref> and <italic>Alyssum murale</italic><xref ref-type="bibr" rid="b21">21</xref><xref ref-type="bibr" rid="b22">22</xref>. Significant enrichment of Ni in phloem exudates from <italic>Noccaea caerulescens</italic> leaves were recently reported<xref ref-type="bibr" rid="b49">49</xref>. These reports and results of our present investigations suggest that high concentration of Ni in phloem could be a common feature in Ni hyperaccumulator plants and this requires more attention in further investigations.</p><p>Preferential accumulation of Ni in vascular tracts suggests that Ni is present in water-soluble form. The chemical speciation of Ni<sup>2+</sup> has been implicated to play a significant role in tissue and cellular-level detoxification, but the observation that Ni<sup>2+</sup> complexes are water-soluble (as evidenced from the phloem sap) ostensibly suggest that Ni<sup>2+</sup> is present in a metabolically active form. Although it has been suggested that Ni<sup>2+</sup> may be complexed with S and N-donors<xref ref-type="bibr" rid="b50">50</xref>, the tissue concentrations of S are orders of magnitude lower than Ni in the three species studied, and unlikely to be important. Nevertheless, in <italic>Alyssum</italic> spp. SO<sub>2</sub><sup>−4</sup> was posited as counter ion for Ni<sup>2+</sup> in vacuoles, rather than S-containing organic ligands<xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b34">34</xref>.</p><p>The results from the XAS analyses here show that Ni<sup>2+</sup> is complexed by carboxylic acids (predominantly citrate) in all three species and in all different tissues and transport liquids analysed. The Ni<sup>2+</sup> chemical speciation differs very little between the species and between the tissues, except for small changes in the relative abundance of Ni:citrate complexes of 1:1 and 1:2 stoichiometry. This aligns with titrimetric determinations of Ni:citrate complexes<xref ref-type="bibr" rid="b51">51</xref>. It, therefore, appears that the chemical speciation of Ni<sup>2+</sup> does not change as it is transported from the roots to the leaves.</p><p>Due to the earlier alluded difficulties of accurately determining the stoichiometry of the carboxylic acids involved using synchrotron XAS alone, we have coupled the analyses with chromatographic methods. The only Ni<sup>2+</sup> complex that could be identified using LC-MS analysis was the Ni<sup>2+</sup> citrate complex [M.L<sub>2</sub>-H]<sup>+</sup>, whereas GC-MS and HPLC-UV analysis quantified the concentrations of carboxylic acids. The phloem sap fluid is ‘biologically relevant’ as it derived directly from sieve elements and hence exists in that form within intact cells. As has been established in other tropical hyperaccumulator plants with Ni-rich phloem tissues, Ni<sup>2+</sup> is complexed by citrate (though note that in <italic>Pycnandra acuminata</italic> this is in laticifer exudates). Citrate is the most abundant carboxylic acid in plants and is produced through the Krebs-cycle and the metabolic cost of citric acid as a ligating agent for Ni<sup>2+</sup> is presumably low. Even though this oxygen-donor has a low association constant with Ni<sup>2+</sup> (logK 5.4), S and N-donors with higher stability constants, such as histidine (logK 8.7) or nicotianamine (logK 16.1), are simply not economic for plants to be produced at sufficient quantities, and are not detected in quantities in our experiments. Furthermore, at the acidic pH of plant cell vacuoles (pH < 5.5), carboxylic acids are better ligands for Ni<sup>2+</sup> than amino acids<xref ref-type="bibr" rid="b52">52</xref>.</p><p>Other studies have found evidence for Ni<sup>2+</sup> complexation with histidine, phytate and phosphate using XAS (for example<xref ref-type="bibr" rid="b50">50</xref><xref ref-type="bibr" rid="b53">53</xref>), however the inability of this method to quantify the stoichiometry between Ni<sup>2+</sup> and ligands means that interpretation of this data is difficult. The role of citrate in Ni hyperaccumulation has long been recognised. Studies by ref. <xref ref-type="bibr" rid="b47">47</xref>, <xref ref-type="bibr" rid="b48">48</xref>, <xref ref-type="bibr" rid="b54">54</xref> and <xref ref-type="bibr" rid="b55">55</xref> showed that in 12 different New Caledonian hyperaccumulator species Ni<sup>2+</sup> was almost exclusively ligated with citrate (and minor malate). The remarkable green latex of <italic>Pycnandra acuminata</italic> latex has been studied numerous times which revealed that 37–99% of Ni<sup>2+</sup> is ligated by citrate<xref ref-type="bibr" rid="b56">56</xref><xref ref-type="bibr" rid="b57">57</xref><xref ref-type="bibr" rid="b58">58</xref>.</p><p>This study has shown how accelerator/ion beam, synchrotron and metabolomics techniques can be combined to iteratively strengthen the understanding of Ni distribution and chemical speciation in hyperaccumulating plant tissues. Ni<sup>2+</sup> ligation with citrate is a common characteristic of the studied hyperaccumulator plants, with extreme accumulation in the phloem bundles in these tropical woody species. As such Ni is concentrated from the foliar phloem bundles into phloem tissue in branches and the main trunk. The levels of concentration of the Ni biopathway through the plants are remarkable, in <italic>P. balgooyi</italic> with a mean of 56 μg g<sup>−1</sup> Ni in the xylem increasing to a mean of 14.9% Ni in the phloem sap. As opposed to xylem, movement of ions through the phloem is bi-directional and could hence facilitate Ni<sup>2+</sup> re-distribution in the hyperaccumulator plant to emerging shoots and leaves (as shown by ref. <xref ref-type="bibr" rid="b49">49</xref> for <italic>Noccaea caerulescens</italic>). It is unknown how the extreme concentrations of Ni in the phloem vessels affect the osmotic pressure of the sieve elements and therefore pressure flow involved with phloem loading and unloading of sugars.</p><p>The weak nature of the Ni<sup>2+</sup> citrate complex means that in a mixed ligand environment with N and S-donors Ni<sup>2+</sup> would cause enzyme function inhibition and hence toxicity. Therefore, physical separation of the Ni<sup>2+</sup> citrate complex from metabolically sensitive cell organs is still necessary. The preferential accumulation in the vacuoles of foliar epidermal cells and associated apoplastic space appears to be typical for most hyperaccumulator plants. In these locations Ni<sup>2+</sup> is separated from photosynthesis at the tissue-level and basic metabolic processes at the cell-level.</p><p>Hyperaccumulation essentially consists of two discrete stages, abnormal uptake behaviour in the root with enhanced translocation, followed by effective tissue and cell-level sequestration. Although the Ni<sup>2+</sup> complex environment throughout the studied hyperaccumulating plants is rather simple (Ni<sup>2+</sup> citrate), there are still major knowledge gaps in understanding the biopathway leading from Ni<sup>2+</sup> uptake in the rhizosphere. The results of this study have shown that once Ni<sup>2+</sup> enters the xylem the bulk chemical speciation of Ni<sup>2+</sup> does not change substantially, however, that raises the question how Ni<sup>2+</sup> is trafficked through root apoplastic and symplastic pathways with such exceptional selectivity. For example, the phloem tissue of <italic>R. bengalensis</italic> contains on average 17 790 μg g<sup>−1</sup> Ni versus on average 30 μg g<sup>−1</sup> Co (or 1:593), while soil concentrations are in the order of 1:10. The differences in the association constants for the citrate complexes of Fe, Ni, Co do not permit selective uptake of Ni<sup>2+ </sup><xref ref-type="bibr" rid="b46">46</xref>. Even if citric acid is involved with ligating Ni<sup>2+</sup> in root exudates, only cell-membrane transport proteins based on S-donors have sufficient selectivity and binding strength to traffic Ni<sup>2+</sup> across the pathways in the root.</p><p>The viability of Ni phytomining depends on the combination of biomass production per time unit and Ni uptake-accumulation of the target plant<xref ref-type="bibr" rid="b59">59</xref>. The maximum Ni concentration in the biomass is important, but the speed at which Ni<sup>2+</sup> is taken up and transferred to the growing plant and incorporated into the biomass is also an important factor. A detailed understanding of this process may allow for manipulations in a phytomining operation to increase Ni<sup>2+</sup> uptake efficiency, for example by application of specific soil amendments.</p></sec><sec disp-level="1"><title>Materials and Methods</title><sec disp-level="2"><title>Collection and bulk analysis of plants</title><p>Plant samples (roots, wood, phloem tissue, leaves and phloem and xylem sap) and rhizosphere soil samples of <italic>P. balgooyi, P. securinegioides</italic> and <italic>R. bengalensis</italic> were collected in the native rainforest habitat in Borneo (near Kinabalu Park in Sabah, Malaysia). Bark, phloem and wood samples were taken from the main trunks of all three species by excising these parts with a sharp stainless steel surgical knife and separating them. Phloem sap was collected with glass capillary tubes (200 μm inner diameter 50 mm long), whereas xylem sap was collected with a handheld vacuum pump from excised branches. Individual tissue samples intended for synchrotron XAS (X-ray Absorption Spectroscopy) analysis were subsequently extracted with a razor blade, placed in polycarbonate cuvettes, covered with Kapton tape and rapidly frozen by immersion in liquid N<sub>2</sub> (−196 °C) in the field. No other processing of samples for XAS, such as homogenization, was undertaken and the samples were maintained at cryogenic temperatures between collection in the field and data collection at the synchrotron. Tissue samples intended for nuclear microprobe (micro-PIXE) analysis were similarly excised, but immediately shock-frozen using a metal mirror technique in which the samples were pressed between a block of Cu-metal cooled by liquid N<sub>2</sub> and a second Cu-metal block attached to a Teflon holder. This ensured extremely fast freezing of the plant tissue samples to prevent cellular damage by ice crystal formation. The samples were then wrapped in Al-foil, and transported in a cryogenic container (kept at −196 °C).</p><p>Parallel samples (~300 mg) of all the types described above were freeze-dried (−50 °C for 24 h) and digested using 4 mL HNO<sub>3</sub> (70%) and 1 mL H<sub>2</sub>O<sub>2</sub> (30%) in a microwave oven and diluted to 30 mL with ultrapure water (Millipore 18.2 MΩ·cm at 25 °C). Air-dried rhizosphere soil samples were similarly analysed, but digested with 6 mL HNO<sub>3</sub> (70%) and 3 mL HCl (30%). These samples then were analysed on ICP-AES (Inductively Coupled Plasma Atomic Emission Spectroscopy) for macro-element and trace elements.</p></sec><sec disp-level="2"><title>Nuclear microprobe (PIXE) elemental mapping</title><p>The frozen tissue samples were freeze-dried in a Leica EM CFD Cryosorption Freeze Dryer (Leica Microsystems AG, Austria). The freeze-drying process followed a long, 208 h programmed cycle to prevent shrinkage of the tissues. Freeze-dried plant tissues were then hand cut with a steel razor blade and mounted on specimen holders covered with 0.5% Formvar film and lightly coated with carbon to prevent charging. Several sections from three replicas of each species and organs were obtained. The most representative were analyzed using the nuclear microprobe. Microanalyses were performed using the nuclear microprobe at the Materials Research Department, iThemba LABS, South Africa. The facility and methodology of measurements of biological materials have been reported elsewhere in detail<xref ref-type="bibr" rid="b60">60</xref><xref ref-type="bibr" rid="b61">61</xref>. To summarize: a proton beam of 3 MeV energy, provided by a 6 MV single-ended Van de Graaff accelerator, was focused to a 3 × 3 μm<sup>2</sup> spot and raster scanned over the areas of interest, using square or rectangular scan patterns with a variable sizes (from 400 μm × 400 μm to 2.7 mm × 2.7 mm) and variable number of pixels (up to 128 × 128). The proton current was restricted to 100–150 pA to minimize specimen beam damage. Particle-induced X-ray emission (PIXE) and proton backscattering spectrometry (BS) were used simultaneously. PIXE spectra were registered with a Si(Li) detector (30 mm<sup>2</sup> active area and 8.5 μm Be window) with an additional 125 μm Be layer as an external absorber. The effective energy resolution of the PIXE system (for the Mn Kα line) was 160 eV, measured for individual spectra. The detector was positioned at a takeoff angle of 135° and a working distance of 24 mm. The X-ray energy range was set between 1 and 40 keV. BS spectra were recorded with an annular Si surface barrier detector (100 μm thick) positioned at an average angle of 176°. Data were acquired in the event-by-event mode. The normalization of results was performed using the integrated beam charge, collected simultaneously from a Faraday cup located behind the specimen and from the insulated specimen holder. In total 25 scans were performed (8 leaves, 9 stems and 8 roots). The results reported in figures and corresponding tables are from representative scans done for roots, stems and leaves, respectively. More experimental details can be found elsewhere<xref ref-type="bibr" rid="b18">18</xref>.</p></sec><sec disp-level="2"><title>Synchrotron (XAS) Ni chemical speciation analysis</title><p>Nickel K-edge XAS spectra of the plant tissue samples and standards were recorded in fluorescence mode at either the Australian National Beamline Facility (ANBF), Beamline 20B (bend magnet) at the Photon Factory (2.5 GeV) KEK, Tsukuba, Japan, or at the XAS beamline at the Australian Synchrotron (AS). At the ANBF, mono-chromation was achieved using a water-cooled channel-cut Si(111) crystal monochromator with a 1 mm beam size in the horizontal and 0.5 mm in the vertical direction, and harmonic rejection was achieved by detuning to 50% intensity. At the AS the X-ray beam was mono-chromated by diffraction from a pair of Si(111) crystals and harmonic rejection was achieved with the use of a Rh-coated mirror.</p><p>At both beamlines fluorescence data were recorded using a Canberra-Eurisys 36-element Ge-detector (the same detector was used at both beamlines) positioned at 90ᵒ to the incident beam, with the sample positioned at 45ᵒ to the incident beam. The X-ray beam energy was calibrated simultaneously with data collection using a Ni metal foil recorded in transmission downstream of the sample, where the first peak of the first derivative was assumed to 8331.6 eV.</p><p>At the ANBF the data were collected with 10 eV steps over the pre-edge region from 8120–8320 eV and 0.25 eV steps over the edge region from 8320–8380 eV followed by increments in k-space of 0.05 Å<sup>−1</sup> until k was equal to either 7 (for the standards) or 12 Å<sup>−1</sup> (for the plant samples). Similar parameters were used at the AS, except that the pre-edge point spacing was 6 eV and the k-space steps were 0.035 Å<sup>−1</sup> to k = 12 Å<sup>−1</sup>.</p><p>All samples and standards were analysed at between 5 and 15 K using a closed-cycle He cryostat and contained in either polycarbonate or poly-lactic acid cuvettes covered with Kapton tape. A total of 17 aqueous Ni<sup>2+</sup> standards were prepared by adding ligands in calculated molar excess (1:6) to Ni<sup>2+</sup> to ensure the formation of Ni-ligand complexes (excepting a standard of Ni:nitrate dissolved in water where no additional ligands were added and a standard prepared with a 1:1 ratio of Ni:citrate which was only analysed at the AS). The solutions were diluted to 5 mM [Ni<sup>2+</sup>] before analysis. The pH of the standards was not adjusted, except for the Ni:citrate standards which were prepared at unadjusted and pH adjusted with NaOH to pH 5.5, and with the exception of histidine and glutathione which were adjusted to pH 8 to allow for full de-protonation and hence coordination via N and S donor atoms respectively). Glycerol was then added (final concentration 33 v/v%) to each standard before flash freezing in liquid nitrogen.</p><p>EXAFS data to k = 12 Å<sup>−1</sup> was subsequently recorded at the AS for 6 standards (citrate (for both 1:1 and 10:1 citrate:Ni ratios), malate, malonate, tartate, and hexaquo), with a ligand:Ni ratio of 10:1 and with pH adjusted to 5.5 using NaOH. A comparison of the XANES spectra with the ANBF data equivalents showed that they were identical within the noise level in each case.</p></sec><sec disp-level="2"><title>Metabolite profiling with GC-MS and LC-MS/HPLC-UV</title><p>GC-MS was used for untargeted metabolite profiling in the plant tissues and phloem sap. Freeze-dried samples were accurately weighed into 2 mL cryo-mill tubes containing ceramic beads then extracted with 1 mL of 50% methanol using the Preselys cryo-mill with the following settings: temperature 5 °C, speed 3800 rpm, time 3 × 30 s. Tubes were centrifuged and supernatant transferred to 2 mL vials for analysis. The GC-MS system used comprised an AS 3000 auto-sampler, a Trace gas chromatograph Ultra and a DSQ quadrupole MS with an EI source (ThermoElectron Corporation, Austin, USA). The MS was tuned according to the manufacturer’s recommended procedure using tris-(perfluorobutyl)-amine (FC43). Derivatives were separated on a 30 m VF-5MS column (0.25 mm, 10 m Integra guard column; 0.25 μm film thickness; Varian Inc., Victoria, Australia) with a helium carrier gas (1 mL min<sup>−1</sup>). For trimethylsilyl (TMS) derivatives the following oven temperature program was used: start temperature at 70 °C followed by a 1 °C min<sup>−1</sup> oven temperature ramp to 76 °C, then a 7 °C min<sup>−1</sup> ramp to 325 °C and finally held at 325 °C for 10 min. For tert-butyldimethylsilylation (TBS) derivatives the following oven program was used: start temperature at 100 °C and hold for 1 min then 1 °C min<sup>−1</sup> ramp to 106 °C followed by a 7 °C min<sup>−1</sup> ramp to 325 °C and finally held for 10 min at 325 °C. The following MS conditions were used: injection temperature 23 °C, MS transfer line 280 °C and ion source 250 °C. Mass spectra were recorded at 2 scan/s, with a scanning range of 70–600 amu. For quantitative analysis of the key high abundant metabolites (malate, citrate, fructose, glucose, sucrose and catechin) standard curves were produces using authentic standards.</p><p>Citrate quantification was carried out using HPLC with UV detection at 210 nm (Dionex Ultimate 3000). Extracts were injected (10 μL) onto a Phenomonex Acclaim OA 150 × 3mm μm column. Samples were eluted using an isocratic mobile phase at 0.4 mL min<sup>−1</sup> containing 100 mM Na<sub>2</sub>SO<sub>4</sub>, pH 2.65 (adjusted with methanesulfonic acid). External calibration standards were used to quantify the total citrate concentrations.</p><p>LC-MS was used to search for potential Ni-complexes in the aqueous extracts. An Agilent 6520 electrospray ionisation quadrupole time of flight (ESI-QTOF) high-resolution mass spectrometer was coupled to and Agilent 1200 series liquid chromatography (LC) system. A 2.1 × 100 mm, 1.8 μm C18 Zorbax Elipse plus (Agilent), reversed phase column was used for chromatography. The LC parameters were as follows: column temperature 30 °C, flow rate 0.4 mL min<sup>−1</sup>, with gradient elution using mobile phase A 0.1% formic acid in water, mobile phase B 0.1% formic acid in acetonitrile. The initial mobile phase composition was 1% B which was changed linearly to 100% B over 10 minutes with a 2 minute hold at 100% B then re-equilibration for 5 min at 2% B, giving a total run time of 17 mins. The ESI source settings were: gas temperature 300 °C, gas flow rate 10 L min<sup>−1</sup>, nebulizer pressure 45 psi, capillary voltage 4000 V, fragmentor 150 V. Instrument was tuned in extended dynamic range mode and spectra collected between 70–1700 m/z at 2 scans/second. Samples were analysed in both positive and negative ionization mode.</p><p>Ion chromatography (IC) was used to determine the concentrations of anions in the <italic>P. balgooyi</italic> phloem sap. The freeze-dried phloem sap was reconstituted in ultrapure water, filtered with a 0.45 μm syringe filter and anions analysed using a Dionex IC instrument. The pH of the <italic>P. balgooyi</italic> phloem sap was determined using a micro-tip pH instrument (TPS, Ag/AgCl with ceramic frit junction).</p></sec><sec disp-level="2"><title>Data processing and statistics</title><p>Quantitative results for the PIXE were obtained by a standardless method using GeoPIXE II software package<xref ref-type="bibr" rid="b62">62</xref><xref ref-type="bibr" rid="b63">63</xref><xref ref-type="bibr" rid="b64">64</xref>. The error estimates were extracted from the error matrix generated in the fit, and the minimum detection limits (MDL) were calculated using the Currie equation<xref ref-type="bibr" rid="b65">65</xref>. Quantitative elemental mapping was performed using Dynamic Analysis method<xref ref-type="bibr" rid="b64">64</xref><xref ref-type="bibr" rid="b66">66</xref><xref ref-type="bibr" rid="b67">67</xref>. This method generates elemental images, which are (i) overlap-resolved, (ii) with subtracted background and (iii) quantitative, <italic>i.e.</italic> in μg g<sup>−1</sup> dry weight units. Maps were complemented by data extracted from arbitrarily selected micro-areas corresponding to separated tissues within roots, stems and leaves. PIXE spectra from these micro-areas were used to obtain average elemental concentrations using a full nonlinear deconvolution procedure of fitting PIXE spectra<xref ref-type="bibr" rid="b62">62</xref><xref ref-type="bibr" rid="b63">63</xref>, with matrix corrections based on thickness and matrix composition obtained from the corresponding BS spectra, fitted with a RUMP simulation package<xref ref-type="bibr" rid="b68">68</xref> with non-Rutherford cross-sections for C, O, N. Elemental concentrations from these areas are also reported in μg g<sup>−1</sup> dry weight.</p><p>Data reduction and analysis of the XAS analysis, including calibration, averaging and background subtraction of all spectra and principal component analysis (PCA), target transformation and linear regression analyses of XANES spectra, were performed using the EXAFSPAK software package (G. N. George, Stanford Synchrotron Radiation Lightsource, Menlo Park, CA, USA). All individual detector channels from repeat scans were first averaged using the EXAFSPAK subroutine “average” and the averaged data was then energy shifted according to the Ni foil calibration described above. Background subtraction was then carried out on the averaged data using default values for the Ni K edge in the EXAFSPAK subroutine “backsub”. PCA, target transformation and linear combination fits of XANES spectra were performed over the Ni K-edge region (8320–8450 eV). Linear combination fits did not provide consistent reliable results as noted above and as such these results are not reported herein. Small changes in the presence or absence of minor component models in the fits lead to significant changes in fit results.</p><p>The GC-MS data were processed using AMDIS (Automated Mass spectral Deconvolution and Identification System; NIST, Gaithersburg, USA) deconvolution was carried out on a chromatogram for each species to identify as many known and unknown chromatographic peaks as possible. The eluting derivatives were identified using the National Institute of Standards and Technology (NIST 2008) commercial mass spectra library, and the public domain mass spectra library the Max-Planck-Institute for Plant Physiology, Golm<xref ref-type="bibr" rid="b69">69</xref>. Processing methods were created using the Xcalibur program (ThermoFinnigan), which determined the areas under each peak, found following deconvolution in AMDIS. The processing method used the mass spectral pattern and RT relative to n-alkanes to enable reliable identification in all samples. In the processing method setup, metabolites, which were not identified through library searching, were labelled with their corresponding RT values. In the LC-MS data ions containing Ni<sup>2+</sup> were identified using the unique theoretical isotope pattern. Peak areas for each Ni<sup>2+</sup> -containing ion were determined using MassHunter quant (Agilent).</p></sec></sec><sec disp-level="1"><title>Additional Information</title><p><bold>How to cite this article</bold>: van der Ent, A. <italic>et al</italic>. Nickel biopathways in tropical nickel hyperaccumulating trees from Sabah (Malaysia). <italic>Sci. Rep.</italic><bold>7</bold>, 41861; doi: 10.1038/srep41861 (2017).</p><p><bold>Publisher's note:</bold> Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.</p></sec><sec sec-type="supplementary-material" id="S1"><title>Supplementary Material</title><supplementary-material id="d32e26" content-type="local-data"><caption><title>Supplementary Information</title></caption><media xlink:href="srep41861-s1.pdf"></media></supplementary-material></sec></body><back><ack><p>We thank Rositti Karim, Sukaibin Sumail (Sabah Parks) and Postar Miun (Sabah Forestry Department) for their help during the fieldwork in Malaysia. We also thank Daniel Dias (Metabolomics Australia, Melbourne University and RMIT), Jin Hee Park (University of Queensland) for analytical support in the laboratory in Australia and Karen Cloete (iThemba LABS, South Africa) for technical assistance. This research was undertaken at the nuclear microprobe facility of iThemba Laboratory for Accelerator Based Sciences in South Africa, and at the Australian National Beamline Facility at the Photon Factory in Japan, operated by the Australian Synchrotron. We acknowledge the High Energy Accelerator Research Organisation (KEK) in Tsukuba, Japan, for operational support. Parts of the research were undertaken at the XAS beamline at the Australian Synchrotron, Victoria, Australia. The French National Research Agency through the national “Investissements d’avenir” program (ANR-10-LABX-21 - LABEX RESSOURCES21) is gratefully acknowledged for funding A. van der Ent’s post-doctoral position in 2014–2015. A. van der Ent is the recipient of a Discovery Early Career Researcher Award (DE160100429) from the Australian Research Council. H.H. 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J.M.P. and W.P. performed cryo-preparation, the micro-PIXE experiments and analysed the PIXE data. A.B. performed the anatomical studies. D.C. conducted the GC/ LC-MS an HPLC measurements and analysed the data. A.V.D.E., D.C., B.N., J.M.P., W.P., and H.H. wrote the manuscript.</p></fn></fn-group></back><floats-group><fig id="f1"><label>Figure 1</label><caption><p><italic>Phyllanthus balgooyi</italic>: exuding phloem sap (<bold>A)</bold>, branch with leaves (<bold>B</bold>), cut branch with phloem tissue (<bold>C</bold>); <italic>Phyllanthus securinegioides</italic>: branch with leaves and seed capsules (<bold>D</bold>), plant with inflorescences (<bold>E</bold>), seed capsules (<bold>F</bold>); <italic>Rinorea bengalensis</italic>: mature tree 22 m high (<bold>G</bold>), excised bark with phloem tissue (<bold>H</bold>), pith of twig (<bold>I</bold>).</p></caption><graphic xlink:href="srep41861-f1"></graphic></fig><fig id="f2"><label>Figure 2</label><caption><title>Elemental maps and morphological structure of root cross-sections of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis.</italic></title><p>Concentration scale in wt% dry weight or in μg g<sup>−1</sup>dry weight. X, xylem; P, phloem; C, cortex; E, epidermis. Scale bar – 100 μm.</p></caption><graphic xlink:href="srep41861-f2"></graphic></fig><fig id="f3"><label>Figure 3</label><caption><title>Elemental maps and morphological structure of stem cross-sections of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis.</italic></title><p>Concentration scale in wt% dry weight or in μg g<sup>−1</sup> dry weight. X, xylem; P, phloem; C, cortex; E, epidermis ; Pd, periderm; VC, vascular cambium; YX, younger xylem; OX, older xylem. Scale bar – 1000 μm.</p></caption><graphic xlink:href="srep41861-f3"></graphic></fig><fig id="f4"><label>Figure 4</label><caption><title>Elemental maps and morphological structure of leaf cross-sections of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis.</italic></title><p>Concentration scale in wt% dry weight (for Ni, Ca, K, S, Cl) and μg g<sup>−1</sup> dry weight (for Mn, Fe). UE, upper epidermis; LE, lower epidermis; X, xylem; P, phloem; PM, palisade mesophyll; SM, spongy mesophyll. Scale bar – 100 μm.</p></caption><graphic xlink:href="srep41861-f4"></graphic></fig><fig id="f5"><label>Figure 5</label><caption><p>Ni K-edge X-ray absorption near edge spectra for <bold>A</bold> 1:10 Ni:citrate in aqueous solution; <bold>B</bold> 1:1 Ni:citrate in aqueous solution; <bold>C</bold><italic>R. bengalensis</italic> mid-vein tissue; <bold>D</bold><italic>R. bengalensis</italic> leaf tissue; <bold>E</bold><italic>R. bengalensis</italic> xylem; <bold>F</bold><italic>R. bengalensis</italic> phloem tissue; <bold>G</bold><italic>P. balgooyi</italic> xylem; <bold>H</bold><italic>P. balgooyi</italic> wood tissue; <bold>I</bold><italic>P. balgooyi</italic> root sheath tissue; <bold>J</bold><italic>P. balgooyi</italic> phloem tissue; <bold>K</bold><italic>P. balgooyi</italic> leaf tissue; <bold>L</bold><italic>P. securinegioides</italic> xylem; <bold>M</bold><italic>P. securinegioides</italic> wood tissue; <bold>N</bold><italic>P. securinegioides</italic> root tissue; <bold>O</bold><italic>P. securinegioides</italic> phloem tissue; <bold>P</bold><italic>P. securinegioides</italic> leaf tissue. Vertical lines are drawn at 8361 and 8400 eV to aid visual comparison of key features in the spectra. The spectra are shifted vertically for clarity.</p></caption><graphic xlink:href="srep41861-f5"></graphic></fig><fig id="f6"><label>Figure 6</label><caption><p>Ni K-edge X-ray absorption near edge spectra for <bold>A</bold><italic>P. balgooyi</italic> phloem tissue (black trace) and 1:1 Ni:citrate in aqueous solution (green trace); <bold>B</bold> Ni:nitrate in aqueous solution (i.e. [Ni(H<sub>2</sub>O)<sub>6</sub>]<sup>2+</sup>-black trace) and 1:10 Ni:citrate in aqueous solution (green trace); <bold>C</bold>, 1:6 Ni:glutathione in aqueous solution at pH ~5–6 (black trace), 1:6 Ni:acetate in aqueous solution at pH ~5–6 (green trace), 1:6 Ni:aconitate in aqueous solution at pH ~5–6 (red trace), 1:6 Ni:succinate in aqueous solution at pH ~5–6 (blue trace)–all traces essentially identical; <bold>D</bold> 1:6 Ni:tartrate in aqueous solution at pH ~5–6 (black trace), 1:6 Ni:malate in aqueous solution at pH ~5–6 (green trace); <bold>E</bold> 1:6 Ni:acetylacetone in aqueous solution at pH ~5–6 (black trace), 1:6 Ni:malonate in aqueous solution at pH ~5–6 (green trace); <bold>F</bold> 1:6 Ni:nicotianamine in aqueous solution at pH ~5–6 (black trace), 1:6 Ni:oxalate in aqueous solution at pH ~5–6 (green trace), 1:6 Ni:phytate in aqueous solution at pH ~5–6 (red trace); <bold>G</bold> NiO<sub>(s)</sub> (black trace) and Ni(OH)<sub>2(s)</sub> (green trace) diluted to 1000 ppm in boron nitride; <bold>H</bold> 1:6 Ni:cysteine in aqueous solution at pH ~8 (black trace), 1:6 Ni:glutathione in aqueous solution at pH ~8 (blue trace), 1:6 Ni:histidine in aqueous solution at pH ~8 (red trace). Vertical lines are drawn at 8361 and 8400 eV to aid visual comparison of key features in the spectra. The spectra are shifted vertically for clarity.</p></caption><graphic xlink:href="srep41861-f6"></graphic></fig><fig id="f7"><label>Figure 7</label><caption><title>Mass spectrum of Ni-citrate [M.L<sub>2</sub>-H]<sup>+</sup> from <italic>P. balgooyi</italic> phloem sap (black trace) and theoretical isotope pattern for Ni-citrate (red boxes).</title></caption><graphic xlink:href="srep41861-f7"></graphic></fig><table-wrap position="float" id="t1"><label>Table 1</label><caption><title>Elemental concentrations in the rhizosphere and in the bedrock in the natural habitat of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis</italic> values as ranges and means in μg g<sup>−1</sup> and <bold>*</bold>wt% dry weight).</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th rowspan="2" align="left" valign="top" charoff="50">Species</th><th rowspan="2" align="center" valign="top" charoff="50">n</th><th colspan="8" align="center" valign="top" charoff="50">Rhizosphere soil chemistry<hr></hr></th></tr><tr><th align="center" valign="top" charoff="50">pH</th><th align="center" valign="top" charoff="50">Mg (total)*</th><th align="center" valign="top" charoff="50">Mg (exch.)</th><th align="center" valign="top" charoff="50">P (total)</th><th align="center" valign="top" charoff="50">K (total)</th><th align="center" valign="top" charoff="50">K (exch.)</th><th align="center" valign="top" charoff="50">Ca (total)</th><th align="center" valign="top" charoff="50">Ca (exch.)</th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">6.4–6.5 [6.4]</td><td align="center" valign="top" charoff="50">6.93–9.92 [8.58]</td><td align="center" valign="top" charoff="50">1040–3520 [1970]</td><td align="center" valign="top" charoff="50">82–180 [140]</td><td align="center" valign="top" charoff="50">153–179 [163]</td><td align="center" valign="top" charoff="50">32–100 [57]</td><td align="center" valign="top" charoff="50">180–1500 [640]</td><td align="center" valign="top" charoff="50">340–3320 [1590]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">9</td><td align="center" valign="top" charoff="50">5.6–7.3 [6.6]</td><td align="center" valign="top" charoff="50">3.33–14.71 [7.56]</td><td align="center" valign="top" charoff="50">510–6120 [2732]</td><td align="center" valign="top" charoff="50">44–590 [200]</td><td align="center" valign="top" charoff="50">36–328 [102]</td><td align="center" valign="top" charoff="50">17–200 [67]</td><td align="center" valign="top" charoff="50">90–7720 [3750]</td><td align="center" valign="top" charoff="50">450–4440 [1600]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">6</td><td align="center" valign="top" charoff="50">6.6–7.6 [7.1]</td><td align="center" valign="top" charoff="50">0.20–5.40 [2.45]</td><td align="center" valign="top" charoff="50">710–4790 [2190]</td><td align="center" valign="top" charoff="50">45–540 [200]</td><td align="center" valign="top" charoff="50">32–144 [91]</td><td align="center" valign="top" charoff="50">21–310 [120]</td><td align="center" valign="top" charoff="50">63–9240 [2820]</td><td align="center" valign="top" charoff="50">360–3460 [1330]</td></tr><tr><td align="left" valign="top" charoff="50"><bold>Species</bold></td><td align="center" valign="top" charoff="50"><bold>n</bold></td><td align="center" valign="top" charoff="50"><bold>Cr (total)</bold></td><td align="center" valign="top" charoff="50"><bold>Mn (total)</bold></td><td align="center" valign="top" charoff="50"><bold>Fe (total)*</bold></td><td align="center" valign="top" charoff="50"><bold>Co (total)</bold></td><td align="center" valign="top" charoff="50"><bold>Co (DTPA)</bold></td><td align="center" valign="top" charoff="50"><bold>Ni (total)</bold></td><td align="center" valign="top" charoff="50"><bold>Ni (DTPA)</bold></td><td align="center" valign="top" charoff="50"><bold>Ni (Sr(NO</bold><sub><bold>3</bold></sub>)<sub><bold>2</bold></sub></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">420–440 [430]</td><td align="center" valign="top" charoff="50">1432–2330 [1770]</td><td align="center" valign="top" charoff="50">5.42–7.71 [6.35]</td><td align="center" valign="top" charoff="50">170–560 [360]</td><td align="center" valign="top" charoff="50">16–68 [40]</td><td align="center" valign="top" charoff="50">1620–3960 [2480]</td><td align="center" valign="top" charoff="50">180–290 [220]</td><td align="center" valign="top" charoff="50">4.2–48 [20]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">9</td><td align="center" valign="top" charoff="50">1240–6050 [3080]</td><td align="center" valign="top" charoff="50">1427–6460 [3010]</td><td align="center" valign="top" charoff="50">4.03–18.55 [9.83]</td><td align="center" valign="top" charoff="50">240–710 [430]</td><td align="center" valign="top" charoff="50">5–24 [15]</td><td align="center" valign="top" charoff="50">1080–5450 [2570]</td><td align="center" valign="top" charoff="50">21–200 [88]</td><td align="center" valign="top" charoff="50">2.1–15 [7.4]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">6</td><td align="center" valign="top" charoff="50">1920–10980 [4710]</td><td align="center" valign="top" charoff="50">2200–7330 [4060]</td><td align="center" valign="top" charoff="50">7.92–39.60 [16.12]</td><td align="center" valign="top" charoff="50">210–690 [350]</td><td align="center" valign="top" charoff="50">4–24 [11]</td><td align="center" valign="top" charoff="50">2160–4410 [3110]</td><td align="center" valign="top" charoff="50">21–440 [150]</td><td align="center" valign="top" charoff="50">3.4–17 [9.2]</td></tr><tr><td rowspan="2" align="left" valign="middle" charoff="50"><bold>Species</bold></td><td rowspan="2" align="center" valign="middle" charoff="50"><bold>n</bold></td><td colspan="8" align="center" valign="top" charoff="50"><bold>Bedrock chemistry</bold><hr></hr></td></tr><tr><td align="center" valign="top" charoff="50"><bold>Na*</bold></td><td align="center" valign="top" charoff="50"><bold>Mg*</bold></td><td align="center" valign="top" charoff="50"><bold>Al*</bold></td><td align="center" valign="top" charoff="50"><bold>Si*</bold></td><td align="center" valign="top" charoff="50"><bold>P</bold></td><td align="center" valign="top" charoff="50"><bold>S*</bold></td><td align="center" valign="top" charoff="50"><bold>K*</bold></td><td align="center" valign="top" charoff="50"><bold>Ca*</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">0.02–0.15 [0.08]</td><td align="center" valign="top" charoff="50">38–46 [42]</td><td align="center" valign="top" charoff="50">0.4–4.5 [2.5]</td><td align="center" valign="top" charoff="50">16–20 [18]</td><td align="center" valign="top" charoff="50">11–72 [42]</td><td align="center" valign="top" charoff="50">0.04–0.1 [0.07]</td><td align="center" valign="top" charoff="50">0.01–0.01 [0.01]</td><td align="center" valign="top" charoff="50">0.8–2.4 [1.6]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">0.01–0.13 [0.06]</td><td align="center" valign="top" charoff="50">7.0–46 [29]</td><td align="center" valign="top" charoff="50">0.2–2.8 [1.3]</td><td align="center" valign="top" charoff="50">13–20 [17]</td><td align="center" valign="top" charoff="50">30–52 [38]</td><td align="center" valign="top" charoff="50">0.05–0.1 [0.07]</td><td align="center" valign="top" charoff="50">0.01–0.03 [0.02]</td><td align="center" valign="top" charoff="50">0.2–1.7 [0.7]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">0.12–0.20 [0.16]</td><td align="center" valign="top" charoff="50">9.0–18 [14]</td><td align="center" valign="top" charoff="50">0.7–2.4 [1.6]</td><td align="center" valign="top" charoff="50">2–21 [12]</td><td align="center" valign="top" charoff="50">38–76 [57]</td><td align="center" valign="top" charoff="50">0.06–0.09 [0.07]</td><td align="center" valign="top" charoff="50">0.01–0.03 [0.02]</td><td align="center" valign="top" charoff="50">1.8–2.6 [2.2]</td></tr><tr><td align="left" valign="top" charoff="50"><bold>Species</bold></td><td align="center" valign="top" charoff="50"><bold>n</bold></td><td align="center" valign="top" charoff="50"><bold>Cr</bold></td><td align="center" valign="top" charoff="50"><bold>Mn</bold></td><td align="center" valign="top" charoff="50"><bold>Fe*</bold></td><td align="center" valign="top" charoff="50"><bold>Co</bold></td><td align="center" valign="top" charoff="50"><bold>Ni</bold></td><td align="center" valign="top" charoff="50"><bold>Cu</bold></td><td align="center" valign="top" charoff="50"><bold>Zn</bold></td><td align="center" valign="top" charoff="50"><bold>Mo</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">750–2830 [1790]</td><td align="center" valign="top" charoff="50">1170–5770 [3470]</td><td align="center" valign="top" charoff="50">6.0–10 [8.2]</td><td align="center" valign="top" charoff="50">9.2–20 [15]</td><td align="center" valign="top" charoff="50">1110–1390 [1250]</td><td align="center" valign="top" charoff="50">0.7–35 [18]</td><td align="center" valign="top" charoff="50">54–120 [85]</td><td align="center" valign="top" charoff="50">1.0–1.5 [1.2]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">850–2230 [1730]</td><td align="center" valign="top" charoff="50">890–2540 [1630]</td><td align="center" valign="top" charoff="50">1.9–8.5 [5.9]</td><td align="center" valign="top" charoff="50">9.0–11 [10]</td><td align="center" valign="top" charoff="50">1030–1610 [1380]</td><td align="center" valign="top" charoff="50">8–14 [10]</td><td align="center" valign="top" charoff="50">49–59 [55]</td><td align="center" valign="top" charoff="50">1.2–1.6 [1.4]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">1661–2630 [2140]</td><td align="center" valign="top" charoff="50">930–1300 [1120]</td><td align="center" valign="top" charoff="50">4.3–4.6 [4.5]</td><td align="center" valign="top" charoff="50">6.8–8.8 [7.8]</td><td align="center" valign="top" charoff="50">970–1220 [1090]</td><td align="center" valign="top" charoff="50">43–50 [47]</td><td align="center" valign="top" charoff="50">46–68 [57]</td><td align="center" valign="top" charoff="50">0.9–1.3 [1.1]</td></tr></tbody></table><table-wrap-foot><fn id="t1-fn1"><p>The digests and extracts were analysed with ICP-AES. Abbreviations: <italic>‘ICP-AES’</italic> is Inductively Coupled Plasma Atomic Emission Spectroscopy), ‘<italic>Total’</italic> is extractable with HCl/HNO<sub>3</sub> digest, ‘<italic>DTPA’</italic> is extractable with Diethylenetriamine pentaacetic acid solution, ‘<italic>Exch</italic>.’ is extractable with silver thiorea solution, and <italic>‘Sr(NO</italic><sub><italic>3</italic></sub>)<sub><italic>2</italic></sub>’ is extractable with strontium nitrate solution.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t2"><label>Table 2</label><caption><title>Bulk elemental concentrations in plant tissues (leaves, twigs, bark, wood) and fluids (xylem and phloem) in <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis</italic> (values as ranges and means in μg g<sup>−1</sup> dry weight).</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50">Species</th><th align="center" valign="top" charoff="50">n</th><th align="center" valign="top" charoff="50">Ni</th><th align="center" valign="top" charoff="50">Mg</th><th align="center" valign="top" charoff="50">Al</th><th align="center" valign="top" charoff="50">P</th><th align="center" valign="top" charoff="50">S</th><th align="center" valign="top" charoff="50">K</th><th align="center" valign="top" charoff="50">Ca</th><th align="center" valign="top" charoff="50">Co</th><th align="center" valign="top" charoff="50">Mn</th><th align="center" valign="top" charoff="50">Zn</th></tr></thead><tbody valign="top"><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Leaves</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">7</td><td align="center" valign="top" charoff="50">1070–8290 [4680]</td><td align="center" valign="top" charoff="50">1060–4980 [3080]</td><td align="center" valign="top" charoff="50">2.0–46 [31]</td><td align="center" valign="top" charoff="50">31–740 [370]</td><td align="center" valign="top" charoff="50">120–1380 [860]</td><td align="center" valign="top" charoff="50">72–364 [860]</td><td align="center" valign="top" charoff="50">230–8680 [4290]</td><td align="center" valign="top" charoff="50">6.0–65 [31]</td><td align="center" valign="top" charoff="50">63–480 [240]</td><td align="center" valign="top" charoff="50">14–150 [88]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">14</td><td align="center" valign="top" charoff="50">10750–25060 [16530]</td><td align="center" valign="top" charoff="50">1060–5500 [3410]</td><td align="center" valign="top" charoff="50">14–69 [31]</td><td align="center" valign="top" charoff="50">430–1690 [740]</td><td align="center" valign="top" charoff="50">1200–2690 [1570]</td><td align="center" valign="top" charoff="50">3100–13380 [5630]</td><td align="center" valign="top" charoff="50">3010–12160 [5790]</td><td align="center" valign="top" charoff="50">12–75 [30]</td><td align="center" valign="top" charoff="50">95–460 [190]</td><td align="center" valign="top" charoff="50">45–250 [92]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">12</td><td align="center" valign="top" charoff="50">2390–8470 [5470]</td><td align="center" valign="top" charoff="50">1470–5130 [3060]</td><td align="center" valign="top" charoff="50">10–70 [26]</td><td align="center" valign="top" charoff="50">420–1190 [790]</td><td align="center" valign="top" charoff="50">1040–2700 [1640]</td><td align="center" valign="top" charoff="50">2160–27990 [8740]</td><td align="center" valign="top" charoff="50">4210–20480 [8020]</td><td align="center" valign="top" charoff="50">10–32 [19]</td><td align="center" valign="top" charoff="50">90–910 [370]</td><td align="center" valign="top" charoff="50">33–280 [95]</td></tr><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Branches</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">2550–8610 [5270]</td><td align="center" valign="top" charoff="50">270–500 [390]</td><td align="center" valign="top" charoff="50">10–5 [29]</td><td align="center" valign="top" charoff="50">140–200 [180]</td><td align="center" valign="top" charoff="50">320–700 [520]</td><td align="center" valign="top" charoff="50">1190–2320 [1780]</td><td align="center" valign="top" charoff="50">630–3740 [2070]</td><td align="center" valign="top" charoff="50">11–46 [26]</td><td align="center" valign="top" charoff="50">19–190 [89]</td><td align="center" valign="top" charoff="50">35–94 [64]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">6</td><td align="center" valign="top" charoff="50">5110–12310 [7630]</td><td align="center" valign="top" charoff="50">330–5280 [1740]</td><td align="center" valign="top" charoff="50">8.0–55 [29]</td><td align="center" valign="top" charoff="50">250–1450 [780]</td><td align="center" valign="top" charoff="50">320–1330 [760]</td><td align="center" valign="top" charoff="50">4920–13250 [8710]</td><td align="center" valign="top" charoff="50">1180–25860 [8290]</td><td align="center" valign="top" charoff="50">5.0–36 [20]</td><td align="center" valign="top" charoff="50">38–150 [94]</td><td align="center" valign="top" charoff="50">46–100 [74]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">4</td><td align="center" valign="top" charoff="50">1810–8510 [5140]</td><td align="center" valign="top" charoff="50">1080–6040 [3010]</td><td align="center" valign="top" charoff="50">5.0–69 [28]</td><td align="center" valign="top" charoff="50">280–1170 [720]</td><td align="center" valign="top" charoff="50">680–2350 [1510]</td><td align="center" valign="top" charoff="50">1990–11430 [6520]</td><td align="center" valign="top" charoff="50">2040–77430 [34410]</td><td align="center" valign="top" charoff="50">9.0–20 [13]</td><td align="center" valign="top" charoff="50">16–110 [57]</td><td align="center" valign="top" charoff="50">27–250 [120]</td></tr><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Wood</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">250–880 [560]</td><td align="center" valign="top" charoff="50">90–250 [150]</td><td align="center" valign="top" charoff="50">14–31 [20]</td><td align="center" valign="top" charoff="50">62–78 [67]</td><td align="center" valign="top" charoff="50">210–280 [240]</td><td align="center" valign="top" charoff="50">330–490 [420]</td><td align="center" valign="top" charoff="50">430–490 [460]</td><td align="center" valign="top" charoff="50">2.0–11 [5.7]</td><td align="center" valign="top" charoff="50">5.0–47 [20]</td><td align="center" valign="top" charoff="50">8.0–14 [11]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">7</td><td align="center" valign="top" charoff="50">440–1170 [860]</td><td align="center" valign="top" charoff="50">85–250 [150]</td><td align="center" valign="top" charoff="50">2.8–23 [14]</td><td align="center" valign="top" charoff="50">25–790 [360]</td><td align="center" valign="top" charoff="50">50–510 [310]</td><td align="center" valign="top" charoff="50">80–2720 [1580]</td><td align="center" valign="top" charoff="50">125–1300 [760]</td><td align="center" valign="top" charoff="50">0.8–15 [5.0]</td><td align="center" valign="top" charoff="50">5.9–18 [11]</td><td align="center" valign="top" charoff="50">4.8–14 [10]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">5</td><td align="center" valign="top" charoff="50">340–1920 [880]</td><td align="center" valign="top" charoff="50">290–650 [470]</td><td align="center" valign="top" charoff="50">6.0–26 [13]</td><td align="center" valign="top" charoff="50">110–360 [210]</td><td align="center" valign="top" charoff="50">250–1100 [440]</td><td align="center" valign="top" charoff="50">510–2720 1470]</td><td align="center" valign="top" charoff="50">1720–5760 [3360]</td><td align="center" valign="top" charoff="50">2.0–14 [4.8]</td><td align="center" valign="top" charoff="50">6.0–21 [13]</td><td align="center" valign="top" charoff="50">7.0–100 [35]</td></tr><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Bark</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">210–370 [310]</td><td align="center" valign="top" charoff="50">170–420 [310]</td><td align="center" valign="top" charoff="50">23–93 [55]</td><td align="center" valign="top" charoff="50">14–25 [21]</td><td align="center" valign="top" charoff="50">71–110 [90]</td><td align="center" valign="top" charoff="50">23–54 [42]</td><td align="center" valign="top" charoff="50">50–120 [90]</td><td align="center" valign="top" charoff="50">2.8–5.2 [4.0]</td><td align="center" valign="top" charoff="50">22–45 [37]</td><td align="center" valign="top" charoff="50">5.9–11 [8.8]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">5</td><td align="center" valign="top" charoff="50">3770–6820 [5860]</td><td align="center" valign="top" charoff="50">400–2160 [870]</td><td align="center" valign="top" charoff="50">14–58 [38]</td><td align="center" valign="top" charoff="50">230–510 [390]</td><td align="center" valign="top" charoff="50">630–1130 [830]</td><td align="center" valign="top" charoff="50">1860–6240 [4420]</td><td align="center" valign="top" charoff="50">1960–23400 [10850]</td><td align="center" valign="top" charoff="50">4.4–14 [9.2]</td><td align="center" valign="top" charoff="50">33–89 [68]</td><td align="center" valign="top" charoff="50">74–83 [78]</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">3300–4630 [3970]</td><td align="center" valign="top" charoff="50">570–650 [610]</td><td align="center" valign="top" charoff="50">49–70 [59]</td><td align="center" valign="top" charoff="50">220–390 [310]</td><td align="center" valign="top" charoff="50">500–770 [640]</td><td align="center" valign="top" charoff="50">2890–3120 [3000]</td><td align="center" valign="top" charoff="50">28260–35530 [31890]</td><td align="center" valign="top" charoff="50">4.9–5.2 [5.1]</td><td align="center" valign="top" charoff="50">25–48 [36]</td><td align="center" valign="top" charoff="50">227–250 [240]</td></tr><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Roots</bold></td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">4780</td><td align="center" valign="top" charoff="50">300</td><td align="center" valign="top" charoff="50">14</td><td align="center" valign="top" charoff="50">95</td><td align="center" valign="top" charoff="50">680</td><td align="center" valign="top" charoff="50">610</td><td align="center" valign="top" charoff="50">410</td><td align="center" valign="top" charoff="50">120</td><td align="center" valign="top" charoff="50">7</td><td align="center" valign="top" charoff="50">120</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">1270</td><td align="center" valign="top" charoff="50">380</td><td align="center" valign="top" charoff="50">91</td><td align="center" valign="top" charoff="50">40</td><td align="center" valign="top" charoff="50">81</td><td align="center" valign="top" charoff="50">53</td><td align="center" valign="top" charoff="50">130</td><td align="center" valign="top" charoff="50">26</td><td align="center" valign="top" charoff="50">16</td><td align="center" valign="top" charoff="50">24</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">—</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td><td align="center" valign="top" charoff="50">n.a.</td></tr></tbody></table><table-wrap-foot><fn id="t2-fn1"><p>The digests and extracts were analysed with ICP-AES.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t3"><label>Table 3</label><caption><title>Bulk elemental concentrations in transport tissue and liquids (xylem and phloem) in <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis</italic> (values as ranges and means in μg g<sup>−1</sup> dry weight).</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="center" valign="top" charoff="50">Species</th><th align="center" valign="top" charoff="50">n</th><th align="center" valign="top" charoff="50">Ni</th><th align="center" valign="top" charoff="50">Mg</th><th align="center" valign="top" charoff="50">Al</th><th align="center" valign="top" charoff="50">P</th><th align="center" valign="top" charoff="50">S</th><th align="center" valign="top" charoff="50">K</th><th align="center" valign="top" charoff="50">Ca</th><th align="center" valign="top" charoff="50">Co</th><th align="center" valign="top" charoff="50">Mn</th><th align="center" valign="top" charoff="50">Zn</th></tr></thead><tbody valign="top"><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Xylem exudate</bold></td></tr><tr><td align="center" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50"><bold>2</bold></td><td align="center" valign="top" charoff="50">35–76 [56]</td><td align="center" valign="top" charoff="50">4.0–11 [7.5]</td><td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50">3.0–7.0 [5.0]</td><td align="center" valign="top" charoff="50">7.0–10.0 [8.5]</td><td align="center" valign="top" charoff="50">44–52 [48]</td><td align="center" valign="top" charoff="50">10–30 [20]</td><td align="center" valign="top" charoff="50">0.01–1.0 [0.5]</td><td align="center" valign="top" charoff="50">1.0–1.0 [1.0]</td><td align="center" valign="top" charoff="50">0.01–1.0 [0.5]</td></tr><tr><td align="center" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50"><bold>1</bold></td><td align="center" valign="top" charoff="50">13</td><td align="center" valign="top" charoff="50">2.0</td><td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50">5.0</td><td align="center" valign="top" charoff="50">2.0</td><td align="center" valign="top" charoff="50">53</td><td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50"></td> </tr><tr><td align="center" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50"><bold>2</bold></td><td align="center" valign="top" charoff="50">6.0–19 [12.5]</td><td align="center" valign="top" charoff="50">3.0–7.0 [5.0]</td><td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50">4.0–5.0 [4.5]</td><td align="center" valign="top" charoff="50">3.0–6.0 [4.5]</td><td align="center" valign="top" charoff="50">28–58 [43]</td><td align="center" valign="top" charoff="50">10–10 [10]</td><td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50"></td> <td align="center" valign="top" charoff="50"></td> </tr><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Phloem sap</bold></td></tr><tr><td align="center" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50"><bold>4</bold></td><td align="center" valign="top" charoff="50">125910–168510 [148730]</td><td align="center" valign="top" charoff="50">490–820 [680]</td><td align="center" valign="top" charoff="50">6.0–30 [19]</td><td align="center" valign="top" charoff="50">23–190 [130]</td><td align="center" valign="top" charoff="50">470–920 [730]</td><td align="center" valign="top" charoff="50">520–1530 [980]</td><td align="center" valign="top" charoff="50">660–1960 [1450]</td><td align="center" valign="top" charoff="50">560–2340 [1550]</td><td align="center" valign="top" charoff="50">320–480 [390]</td><td align="center" valign="top" charoff="50">1900–3690 [2580]</td></tr><tr><td colspan="12" align="center" valign="top" charoff="50"><bold>Phloem tissue</bold></td></tr><tr><td align="center" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50"><bold>4</bold></td><td align="center" valign="top" charoff="50">45010–79340 [65410]</td><td align="center" valign="top" charoff="50">600–1160 [890]</td><td align="center" valign="top" charoff="50">12–18 [15]</td><td align="center" valign="top" charoff="50">140–240 [220]</td><td align="center" valign="top" charoff="50">1310–2150 [1850]</td><td align="center" valign="top" charoff="50">2700–4770 [3320]</td><td align="center" valign="top" charoff="50">2920–4410 [3570]</td><td align="center" valign="top" charoff="50">190–1170 [575]</td><td align="center" valign="top" charoff="50">160–280 [200]</td><td align="center" valign="top" charoff="50">410–1930 [960]</td></tr><tr><td align="center" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50"><bold>5</bold></td><td align="center" valign="top" charoff="50">5990–10800 [8940]</td><td align="center" valign="top" charoff="50">1060–1360 [1230]</td><td align="center" valign="top" charoff="50">16–29 [25]</td><td align="center" valign="top" charoff="50">170–340 [260]</td><td align="center" valign="top" charoff="50">950–2740 [1760]</td><td align="center" valign="top" charoff="50">3670–8420 [5650]</td><td align="center" valign="top" charoff="50">12860–51510 [38820]</td><td align="center" valign="top" charoff="50">12–24 [19]</td><td align="center" valign="top" charoff="50">62–130 [87]</td><td align="center" valign="top" charoff="50">67–190 [97]</td></tr><tr><td align="center" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50"><bold>7</bold></td><td align="center" valign="top" charoff="50">13630–22600 [17790]</td><td align="center" valign="top" charoff="50">650–3020 [1380]</td><td align="center" valign="top" charoff="50">12–27 [20]</td><td align="center" valign="top" charoff="50">260–760 [470]</td><td align="center" valign="top" charoff="50">450–4630 [2960]</td><td align="center" valign="top" charoff="50">4770–8670 [7270]</td><td align="center" valign="top" charoff="50">3670–37210 [22570]</td><td align="center" valign="top" charoff="50">4–58 [30]</td><td align="center" valign="top" charoff="50">29–290 [290]</td><td align="center" valign="top" charoff="50">210–770 [510]</td></tr></tbody></table><table-wrap-foot><fn id="t3-fn1"><p>The digests were analysed with ICP-AES.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t4"><label>Table 4</label><caption><title>Elemental concentrations (micro-PIXE, μg g<sup>−1</sup> dry weight) in the morphological structures of the roots of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis</italic>.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50"> </th><th align="center" valign="top" charoff="50">Ni</th><th align="center" valign="top" charoff="50">Si</th><th align="center" valign="top" charoff="50">P</th><th align="center" valign="top" charoff="50">S</th><th align="center" valign="top" charoff="50">Cl</th><th align="center" valign="top" charoff="50">K</th><th align="center" valign="top" charoff="50">Ca</th><th align="center" valign="top" charoff="50">Mn</th><th align="center" valign="top" charoff="50">Fe</th><th align="center" valign="top" charoff="50">Co</th><th align="center" valign="top" charoff="50">Cu</th><th align="center" valign="top" charoff="50">Zn</th></tr></thead><tbody valign="top"><tr><td colspan="13" align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td></tr><tr><td align="left" valign="top" charoff="50"> Whole area</td><td align="center" valign="top" charoff="50">120 (4)</td><td align="center" valign="top" charoff="50">2600 (180)</td><td align="center" valign="top" charoff="50">100 (16)</td><td align="center" valign="top" charoff="50">1310 (40)</td><td align="center" valign="top" charoff="50">5040 (12)</td><td align="center" valign="top" charoff="50">6870 (50)</td><td align="center" valign="top" charoff="50">410 (23)</td><td align="center" valign="top" charoff="50">29 (3)</td><td align="center" valign="top" charoff="50">710 (15)</td><td align="center" valign="top" charoff="50">7 (3)</td><td align="center" valign="top" charoff="50">3.1 (0.5)</td><td align="center" valign="top" charoff="50">10.4 (0.7)</td></tr><tr><td align="left" valign="top" charoff="50"> Epidermis</td><td align="center" valign="top" charoff="50">180 (14)</td><td align="center" valign="top" charoff="50">35010 (14)</td><td align="center" valign="top" charoff="50"><113</td><td align="center" valign="top" charoff="50">1120 (50)</td><td align="center" valign="top" charoff="50">320 (25)</td><td align="center" valign="top" charoff="50">2380 (50)</td><td align="center" valign="top" charoff="50">860 (27)</td><td align="center" valign="top" charoff="50">63 (9)</td><td align="center" valign="top" charoff="50">3540 (50)</td><td align="center" valign="top" charoff="50">17 (12)</td><td align="center" valign="top" charoff="50"><15</td><td align="center" valign="top" charoff="50">15 (7)</td></tr><tr><td align="left" valign="top" charoff="50"> Cortex</td><td align="center" valign="top" charoff="50">34 (3)</td><td align="center" valign="top" charoff="50">450 (3)</td><td align="center" valign="top" charoff="50">90 (20)</td><td align="center" valign="top" charoff="50">840 (20)</td><td align="center" valign="top" charoff="50">7780 (20)</td><td align="center" valign="top" charoff="50">7950 (70)</td><td align="center" valign="top" charoff="50">380 (26)</td><td align="center" valign="top" charoff="50">9 (2)</td><td align="center" valign="top" charoff="50">163 (8)</td><td align="center" valign="top" charoff="50"><2</td><td align="center" valign="top" charoff="50"><2.1</td><td align="center" valign="top" charoff="50">10 (1)</td></tr><tr><td align="left" valign="top" charoff="50"> Primary phloem</td><td align="center" valign="top" charoff="50">1880 (30)</td><td align="center" valign="top" charoff="50"><385</td><td align="center" valign="top" charoff="50">330 (60)</td><td align="center" valign="top" charoff="50">1320 (40)</td><td align="center" valign="top" charoff="50">1550 (30)</td><td align="center" valign="top" charoff="50">10480 (40)</td><td align="center" valign="top" charoff="50">900 (24)</td><td align="center" valign="top" charoff="50">29 (4)</td><td align="center" valign="top" charoff="50"><10</td><td align="center" valign="top" charoff="50">9 (5)</td><td align="center" valign="top" charoff="50"><22</td><td align="center" valign="top" charoff="50">23 (6)</td></tr><tr><td align="left" valign="top" charoff="50"> Primary xylem</td><td align="center" valign="top" charoff="50">25 (5)</td><td align="center" valign="top" charoff="50"><160</td><td align="center" valign="top" charoff="50">230 (36)</td><td align="center" valign="top" charoff="50">610 (40)</td><td align="center" valign="top" charoff="50">890 (30)</td><td align="center" valign="top" charoff="50">4470 (30)</td><td align="center" valign="top" charoff="50">150 (20)</td><td align="center" valign="top" charoff="50"><4</td><td align="center" valign="top" charoff="50"><4</td><td align="center" valign="top" charoff="50"><3</td><td align="center" valign="top" charoff="50"><6</td><td align="center" valign="top" charoff="50"><8</td></tr><tr><td colspan="13" align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td></tr><tr><td align="left" valign="top" charoff="50"> Whole area</td><td align="center" valign="top" charoff="50">2080 (43)</td><td align="center" valign="top" charoff="50">8170 (470)</td><td align="center" valign="top" charoff="50">240 (32)</td><td align="center" valign="top" charoff="50">2420 (100)</td><td align="center" valign="top" charoff="50">570 (40)</td><td align="center" valign="top" charoff="50">4400 (80)</td><td align="center" valign="top" charoff="50">820 (20)</td><td align="center" valign="top" charoff="50">44 (2)</td><td align="center" valign="top" charoff="50">1230 (20)</td><td align="center" valign="top" charoff="50">6 (4)</td><td align="center" valign="top" charoff="50">6 (2)</td><td align="center" valign="top" charoff="50">113 (2)</td></tr><tr><td align="left" valign="top" charoff="50"> Epidermis</td><td align="center" valign="top" charoff="50">670 (13)</td><td align="center" valign="top" charoff="50">34600 (1500)</td><td align="center" valign="top" charoff="50">75 (24)</td><td align="center" valign="top" charoff="50">1320 (20)</td><td align="center" valign="top" charoff="50">360 (16)</td><td align="center" valign="top" charoff="50">1590 (40)</td><td align="center" valign="top" charoff="50">460 (10)</td><td align="center" valign="top" charoff="50">230 (8)</td><td align="center" valign="top" charoff="50">8330 (110)</td><td align="center" valign="top" charoff="50">50 (13)</td><td align="center" valign="top" charoff="50"><11</td><td align="center" valign="top" charoff="50">18 (4)</td></tr><tr><td align="left" valign="top" charoff="50"> Cortex</td><td align="center" valign="top" charoff="50">2910 (50)</td><td align="center" valign="top" charoff="50">1350 (180)</td><td align="center" valign="top" charoff="50">330 (30)</td><td align="center" valign="top" charoff="50">3520 (130)</td><td align="center" valign="top" charoff="50">750 (30)</td><td align="center" valign="top" charoff="50">3300 (50)</td><td align="center" valign="top" charoff="50">250 (10)</td><td align="center" valign="top" charoff="50">24 (3)</td><td align="center" valign="top" charoff="50">440 (7)</td><td align="center" valign="top" charoff="50"><3</td><td align="center" valign="top" charoff="50">8 (3)</td><td align="center" valign="top" charoff="50">47 (5)</td></tr><tr><td align="left" valign="top" charoff="50"> Phloem</td><td align="center" valign="top" charoff="50">9250 (120)</td><td align="center" valign="top" charoff="50">190 (40)</td><td align="center" valign="top" charoff="50">450 (50)</td><td align="center" valign="top" charoff="50">5220 (240)</td><td align="center" valign="top" charoff="50">1360 (60)</td><td align="center" valign="top" charoff="50">11960 (120)</td><td align="center" valign="top" charoff="50">3250 (40)</td><td align="center" valign="top" charoff="50">7 (2)</td><td align="center" valign="top" charoff="50">47 (3)</td><td align="center" valign="top" charoff="50">7 (6)</td><td align="center" valign="top" charoff="50">10 (5)</td><td align="center" valign="top" charoff="50">570 (12)</td></tr><tr><td align="left" valign="top" charoff="50"> Xylem</td><td align="center" valign="top" charoff="50">340 (9)</td><td align="center" valign="top" charoff="50">390 (40)</td><td align="center" valign="top" charoff="50">300 (30)</td><td align="center" valign="top" charoff="50">1120 (50)</td><td align="center" valign="top" charoff="50">230 (12)</td><td align="center" valign="top" charoff="50">2700 (20)</td><td align="center" valign="top" charoff="50">280 (9)</td><td align="center" valign="top" charoff="50">2.8 (0.7)</td><td align="center" valign="top" charoff="50">14.7 (0.5)</td><td align="center" valign="top" charoff="50"><0.6</td><td align="center" valign="top" charoff="50">2.1 (0.5)</td><td align="center" valign="top" charoff="50">17.7 (0.7)</td></tr><tr><td colspan="13" align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td></tr><tr><td align="left" valign="top" charoff="50"> Whole area</td><td align="center" valign="top" charoff="50">14700 (150)</td><td align="center" valign="top" charoff="50">6310 (650)</td><td align="center" valign="top" charoff="50">130 (23)</td><td align="center" valign="top" charoff="50">1580 (90)</td><td align="center" valign="top" charoff="50">370 (20)</td><td align="center" valign="top" charoff="50">5570 (60)</td><td align="center" valign="top" charoff="50">5460 (50)</td><td align="center" valign="top" charoff="50">25 (2)</td><td align="center" valign="top" charoff="50">920 (15)</td><td align="center" valign="top" charoff="50"><5</td><td align="center" valign="top" charoff="50">25 (7)</td><td align="center" valign="top" charoff="50">63 (2)</td></tr><tr><td align="left" valign="top" charoff="50"> Epidermis</td><td align="center" valign="top" charoff="50">270 (8)</td><td align="center" valign="top" charoff="50">11860 (800)</td><td align="center" valign="top" charoff="50">73 (14)</td><td align="center" valign="top" charoff="50">480 (12)</td><td align="center" valign="top" charoff="50">220 (13)</td><td align="center" valign="top" charoff="50">780 (17)</td><td align="center" valign="top" charoff="50">590 (10)</td><td align="center" valign="top" charoff="50">70 (3)</td><td align="center" valign="top" charoff="50">3170 (50)</td><td align="center" valign="top" charoff="50">13 (5)</td><td align="center" valign="top" charoff="50"><3</td><td align="center" valign="top" charoff="50">29 (2)</td></tr><tr><td align="left" valign="top" charoff="50"> Cortex</td><td align="center" valign="top" charoff="50">10060 (140)</td><td align="center" valign="top" charoff="50"><128</td><td align="center" valign="top" charoff="50">220 (30)</td><td align="center" valign="top" charoff="50">2020 (110)</td><td align="center" valign="top" charoff="50">480 (20)</td><td align="center" valign="top" charoff="50">9460 (130)</td><td align="center" valign="top" charoff="50">5530 (100)</td><td align="center" valign="top" charoff="50">5 (3)</td><td align="center" valign="top" charoff="50">132 (5)</td><td align="center" valign="top" charoff="50"><5</td><td align="center" valign="top" charoff="50">21 (8)</td><td align="center" valign="top" charoff="50">23 (4)</td></tr><tr><td align="left" valign="top" charoff="50"> Phloem</td><td align="center" valign="top" charoff="50">56400 (400)</td><td align="center" valign="top" charoff="50"><110</td><td align="center" valign="top" charoff="50">93 (25)</td><td align="center" valign="top" charoff="50">1450 (80)</td><td align="center" valign="top" charoff="50">690 (13)</td><td align="center" valign="top" charoff="50">10560 (80)</td><td align="center" valign="top" charoff="50">8410 (60)</td><td align="center" valign="top" charoff="50">33 (3)</td><td align="center" valign="top" charoff="50">67 (2)</td><td align="center" valign="top" charoff="50"><11</td><td align="center" valign="top" charoff="50">78 (21)</td><td align="center" valign="top" charoff="50">250 (20)</td></tr><tr><td align="left" valign="top" charoff="50"> Xylem</td><td align="center" valign="top" charoff="50">910 (14)</td><td align="center" valign="top" charoff="50">260 (14)</td><td align="center" valign="top" charoff="50">220 (20)</td><td align="center" valign="top" charoff="50">420 (25)</td><td align="center" valign="top" charoff="50">140 (8)</td><td align="center" valign="top" charoff="50">2660 (20)</td><td align="center" valign="top" charoff="50">1150 (8)</td><td align="center" valign="top" charoff="50">2.6 (0.6)</td><td align="center" valign="top" charoff="50">6.4 (0.5)</td><td align="center" valign="top" charoff="50"><0.8</td><td align="center" valign="top" charoff="50">3.6 (0.6)</td><td align="center" valign="top" charoff="50">13.5 (0.8)</td></tr></tbody></table><table-wrap-foot><fn id="t4-fn1"><p>The results were obtained from PIXE spectra extracted from regions representing different morphological structures, and analysed using GeoPIXE software. Errors of analysis (±1 σ uncertainty) are shown in brackets.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t5"><label>Table 5</label><caption><title>Elemental concentrations (micro-PIXE, μg g<sup>−1</sup> dry weight) in the morphological structures of the stems of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis</italic>.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50"> </th><th align="center" valign="top" charoff="50">Ni</th><th align="center" valign="top" charoff="50">Si</th><th align="center" valign="top" charoff="50">P</th><th align="center" valign="top" charoff="50">S</th><th align="center" valign="top" charoff="50">Cl</th><th align="center" valign="top" charoff="50">K</th><th align="center" valign="top" charoff="50">Ca</th><th align="center" valign="top" charoff="50">Mn</th><th align="center" valign="top" charoff="50">Fe</th><th align="center" valign="top" charoff="50">Co</th><th align="center" valign="top" charoff="50">Cu</th><th align="center" valign="top" charoff="50">Zn</th></tr></thead><tbody valign="top"><tr><td colspan="13" align="left" valign="top" charoff="50"><italic><bold>Phyllanthus balgooyi</bold></italic></td></tr><tr><td align="left" valign="top" charoff="50">Whole area</td><td align="center" valign="top" charoff="50">6860 (80)</td><td align="center" valign="top" charoff="50"><45</td><td align="center" valign="top" charoff="50">315 (16)</td><td align="center" valign="top" charoff="50">1510 (50)</td><td align="center" valign="top" charoff="50">2970 (60)</td><td align="center" valign="top" charoff="50">5280 (50)</td><td align="center" valign="top" charoff="50">1920 (23)</td><td align="center" valign="top" charoff="50">115 (3)</td><td align="center" valign="top" charoff="50">13 (1)</td><td align="center" valign="top" charoff="50">22 (3)</td><td align="center" valign="top" charoff="50">22 (4)</td><td align="center" valign="top" charoff="50">56 (2)</td></tr><tr><td align="left" valign="top" charoff="50">Outer periderm</td><td align="center" valign="top" charoff="50">2010 (30)</td><td align="center" valign="top" charoff="50">170 (55)</td><td align="center" valign="top" charoff="50">310 (23)</td><td align="center" valign="top" charoff="50">940 (40)</td><td align="center" valign="top" charoff="50">970 (20)</td><td align="center" valign="top" charoff="50">3740 (30)</td><td align="center" valign="top" charoff="50">4100 (26)</td><td align="center" valign="top" charoff="50">331 (3)</td><td align="center" valign="top" charoff="50">28 (1)</td><td align="center" valign="top" charoff="50">12 (2)</td><td align="center" valign="top" charoff="50">17 (2)</td><td align="center" valign="top" charoff="50">47 (3)</td></tr><tr><td align="left" valign="top" charoff="50">Inner periderm</td><td align="center" valign="top" charoff="50">4150 (60)</td><td align="center" valign="top" charoff="50">280 (60)</td><td align="center" valign="top" charoff="50">260 (26)</td><td align="center" valign="top" charoff="50">860 (25)</td><td align="center" valign="top" charoff="50">6500 (90)</td><td align="center" valign="top" charoff="50">14680 (120)</td><td align="center" valign="top" charoff="50">2040 (50)</td><td align="center" valign="top" charoff="50">119 (4)</td><td align="center" valign="top" charoff="50"><3</td><td align="center" valign="top" charoff="50">27 (3)</td><td align="center" valign="top" charoff="50">22 (4)</td><td align="center" valign="top" charoff="50">66 (4)</td></tr><tr><td align="left" valign="top" charoff="50">Cortex</td><td align="center" valign="top" charoff="50">12800 (170)</td><td align="center" valign="top" charoff="50"><125</td><td align="center" valign="top" charoff="50">260 (15)</td><td align="center" valign="top" charoff="50">910 (40)</td><td align="center" valign="top" charoff="50">5700 (100)</td><td align="center" valign="top" charoff="50">7570 (80)</td><td align="center" valign="top" charoff="50">1400 (20)</td><td align="center" valign="top" charoff="50">101 (5)</td><td align="center" valign="top" charoff="50">14 (3)</td><td align="center" valign="top" charoff="50">40 (6)</td><td align="center" valign="top" charoff="50">34 (8)</td><td align="center" valign="top" charoff="50">106 (4)</td></tr><tr><td align="left" valign="top" charoff="50">Phloem</td><td align="center" valign="top" charoff="50">86300 (400)</td><td align="center" valign="top" charoff="50">n.d.</td><td align="center" valign="top" charoff="50"><300</td><td align="center" valign="top" charoff="50">1060 (50)</td><td align="center" valign="top" charoff="50">4200 (90)</td><td align="center" valign="top" charoff="50">6930 (50)</td><td align="center" valign="top" charoff="50">2680 (24)</td><td align="center" valign="top" charoff="50">202 (11)</td><td align="center" valign="top" charoff="50">43 (12)</td><td align="center" valign="top" charoff="50">265 (26)</td><td align="center" valign="top" charoff="50"><200</td><td align="center" valign="top" charoff="50">405 (27)</td></tr><tr><td align="left" valign="top" charoff="50">Xylem</td><td align="center" valign="top" charoff="50">1450 (22)</td><td align="center" valign="top" charoff="50">260 (40)</td><td align="center" valign="top" charoff="50">350 (40)</td><td align="center" valign="top" charoff="50">760 (40)</td><td align="center" valign="top" charoff="50">680 (16)</td><td align="center" valign="top" charoff="50">2350 (24)</td><td align="center" valign="top" charoff="50">760 (9)</td><td align="center" valign="top" charoff="50">31.2 (0.9)</td><td align="center" valign="top" charoff="50">7 (1)</td><td align="center" valign="top" charoff="50">3 (1)</td><td align="center" valign="top" charoff="50">11 (1)</td><td align="center" valign="top" charoff="50">12 (1)</td></tr><tr><td align="left" valign="top" charoff="50">Pith</td><td align="center" valign="top" charoff="50">840 (19)</td><td align="center" valign="top" charoff="50"><93</td><td align="center" valign="top" charoff="50">370 (30)</td><td align="center" valign="top" charoff="50">3000 (80)</td><td align="center" valign="top" charoff="50">3710 (30)</td><td align="center" valign="top" charoff="50">4570 (60)</td><td align="center" valign="top" charoff="50">1330 (22)</td><td align="center" valign="top" charoff="50">40 (1)</td><td align="center" valign="top" charoff="50">1.8 (0.8)</td><td align="center" valign="top" charoff="50">2 (1)</td><td align="center" valign="top" charoff="50">10 (1)</td><td align="center" valign="top" charoff="50">13 (1)</td></tr><tr><td colspan="13" align="left" valign="top" charoff="50"><italic><bold>Phyllanthus securinegioides</bold></italic><hr></hr></td></tr><tr><td align="left" valign="top" charoff="50">Whole area</td><td align="center" valign="top" charoff="50">1040 (12)</td><td align="center" valign="top" charoff="50">1840 (200)</td><td align="center" valign="top" charoff="50">365 (20)</td><td align="center" valign="top" charoff="50">830 (50)</td><td align="center" valign="top" charoff="50">730 (11)</td><td align="center" valign="top" charoff="50">8550 (100)</td><td align="center" valign="top" charoff="50">6660 (70)</td><td align="center" valign="top" charoff="50">48 (3)</td><td align="center" valign="top" charoff="50">335 (8)</td><td align="center" valign="top" charoff="50">5 (3)</td><td align="center" valign="top" charoff="50"><2</td><td align="center" valign="top" charoff="50">14.1 (0.7)</td></tr><tr><td align="left" valign="top" charoff="50">Epidermis</td><td align="center" valign="top" charoff="50">700 (24)</td><td align="center" valign="top" charoff="50">42600 (1800)</td><td align="center" valign="top" charoff="50">130 (60)</td><td align="center" valign="top" charoff="50">640 (20)</td><td align="center" valign="top" charoff="50">160 (12)</td><td align="center" valign="top" charoff="50">2360 (60)</td><td align="center" valign="top" charoff="50">2080 (40)</td><td align="center" valign="top" charoff="50">460 (26)</td><td align="center" valign="top" charoff="50">10910 (180)</td><td align="center" valign="top" charoff="50">47 (20)</td><td align="center" valign="top" charoff="50"><12</td><td align="center" valign="top" charoff="50">26 (6)</td></tr><tr><td align="left" valign="top" charoff="50">Cortex</td><td align="center" valign="top" charoff="50">960 (20)</td><td align="center" valign="top" charoff="50">1060 (200)</td><td align="center" valign="top" charoff="50">270 (40)</td><td align="center" valign="top" charoff="50">770 (50)</td><td align="center" valign="top" charoff="50">2560 (40)</td><td align="center" valign="top" charoff="50">6630 (30)</td><td align="center" valign="top" charoff="50">2130 (30)</td><td align="center" valign="top" charoff="50">109 (6)</td><td align="center" valign="top" charoff="50">1460 (23)</td><td align="center" valign="top" charoff="50">18 (5)</td><td align="center" valign="top" charoff="50"><9</td><td align="center" valign="top" charoff="50">24 (6)</td></tr><tr><td align="left" valign="top" charoff="50">Secondary phloem</td><td align="center" valign="top" charoff="50">4150 (60)</td><td align="center" valign="top" charoff="50">1090 (220)</td><td align="center" valign="top" charoff="50">90 (70)</td><td align="center" valign="top" charoff="50">2340 (140)</td><td align="center" valign="top" charoff="50">2280 (50)</td><td align="center" valign="top" charoff="50">14540 (210)</td><td align="center" valign="top" charoff="50">35100 (320)</td><td align="center" valign="top" charoff="50">67(4)</td><td align="center" valign="top" charoff="50">15 (4)</td><td align="center" valign="top" charoff="50"><9</td><td align="center" valign="top" charoff="50"><8</td><td align="center" valign="top" charoff="50">25 (3)</td></tr><tr><td align="left" valign="top" charoff="50">Vascular cambium</td><td align="center" valign="top" charoff="50">530 (23)</td><td align="center" valign="top" charoff="50">920 (140)</td><td align="center" valign="top" charoff="50">1460 (100)</td><td align="center" valign="top" charoff="50">1420 (70)</td><td align="center" valign="top" charoff="50">825 (40)</td><td align="center" valign="top" charoff="50">28800 (100)</td><td align="center" valign="top" charoff="50">2390 (60)</td><td align="center" valign="top" charoff="50">39 (3)</td><td align="center" valign="top" charoff="50">11 (3)</td><td align="center" valign="top" charoff="50"><9</td><td align="center" valign="top" charoff="50">8 (3)</td><td align="center" valign="top" charoff="50">37 (3)</td></tr><tr><td align="left" valign="top" charoff="50">Older xylem</td><td align="center" valign="top" charoff="50">140 (5)</td><td align="center" valign="top" charoff="50">750 (100)</td><td align="center" valign="top" charoff="50">440 (60)</td><td align="center" valign="top" charoff="50">336 (18)</td><td align="center" valign="top" charoff="50">106 (10)</td><td align="center" valign="top" charoff="50">5240 (20)</td><td align="center" valign="top" charoff="50">500 (10)</td><td align="center" valign="top" charoff="50">10.6 (0.9)</td><td align="center" valign="top" charoff="50">11 (0.8)</td><td align="center" valign="top" charoff="50"><1.1</td><td align="center" valign="top" charoff="50">3.3 (0.9)</td><td align="center" valign="top" charoff="50">7.8 (0.9)</td></tr><tr><td align="left" valign="top" charoff="50">Younger xylem</td><td align="center" valign="top" charoff="50">500 (12)</td><td align="center" valign="top" charoff="50">800 (120)</td><td align="center" valign="top" charoff="50">300 (50)</td><td align="center" valign="top" charoff="50">325 (30)</td><td align="center" valign="top" charoff="50">49 (7)</td><td align="center" valign="top" charoff="50">3760 (14)</td><td align="center" valign="top" charoff="50">530 (14)</td><td align="center" valign="top" charoff="50">14.1 (0.9)</td><td align="center" valign="top" charoff="50">21 (1)</td><td align="center" valign="top" charoff="50"><1.2</td><td align="center" valign="top" charoff="50">3.2 (0.9)</td><td align="center" valign="top" charoff="50">5 (1)</td></tr><tr><td align="left" valign="top" charoff="50">Compressed pith</td><td align="center" valign="top" charoff="50">2830 (80)</td><td align="center" valign="top" charoff="50">830 (210)</td><td align="center" valign="top" charoff="50">370 (110)</td><td align="center" valign="top" charoff="50">770 (50)</td><td align="center" valign="top" charoff="50">58 (26)</td><td align="center" valign="top" charoff="50">8810 (80)</td><td align="center" valign="top" charoff="50">4050 (50)</td><td align="center" valign="top" charoff="50">62 (8)</td><td align="center" valign="top" charoff="50"><9</td><td align="center" valign="top" charoff="50"><11</td><td align="center" valign="top" charoff="50"><18</td><td align="center" valign="top" charoff="50">48 (11)</td></tr><tr><td colspan="13" align="left" valign="top" charoff="50"><italic><bold>Rinorea bengalensis</bold></italic><hr></hr></td></tr><tr><td align="left" valign="top" charoff="50">Whole area</td><td align="center" valign="top" charoff="50">1780 (11)</td><td align="center" valign="top" charoff="50">1680 (300)</td><td align="center" valign="top" charoff="50">1550 (170)</td><td align="center" valign="top" charoff="50">760 (40)</td><td align="center" valign="top" charoff="50">120 (20)</td><td align="center" valign="top" charoff="50">11150 (180)</td><td align="center" valign="top" charoff="50">5720 (90)</td><td align="center" valign="top" charoff="50">15 (1)</td><td align="center" valign="top" charoff="50">170 (7)</td><td align="center" valign="top" charoff="50">4 (4)</td><td align="center" valign="top" charoff="50">7 (2)</td><td align="center" valign="top" charoff="50">40 (2)</td></tr><tr><td align="left" valign="top" charoff="50">Epidermis/phellem</td><td align="center" valign="top" charoff="50">1430 (70)</td><td align="center" valign="top" charoff="50">3530 (330)</td><td align="center" valign="top" charoff="50">300 (40)</td><td align="center" valign="top" charoff="50">640 (40)</td><td align="center" valign="top" charoff="50">76 (12)</td><td align="center" valign="top" charoff="50">5030 (160)</td><td align="center" valign="top" charoff="50">12180 (120)</td><td align="center" valign="top" charoff="50">122 (9)</td><td align="center" valign="top" charoff="50">2340 (100)</td><td align="center" valign="top" charoff="50">14 (11)</td><td align="center" valign="top" charoff="50"><8.</td><td align="center" valign="top" charoff="50">21 (4)</td></tr><tr><td align="left" valign="top" charoff="50">Cortex</td><td align="center" valign="top" charoff="50">3070 (40)</td><td align="center" valign="top" charoff="50">1960 (380)</td><td align="center" valign="top" charoff="50">370 (80)</td><td align="center" valign="top" charoff="50">1180 (80)</td><td align="center" valign="top" charoff="50">165 (25)</td><td align="center" valign="top" charoff="50">22420 (190)</td><td align="center" valign="top" charoff="50">4980 (80)</td><td align="center" valign="top" charoff="50">12 (1)</td><td align="center" valign="top" charoff="50">19 (2)</td><td align="center" valign="top" charoff="50"><9</td><td align="center" valign="top" charoff="50">6 (3)</td><td align="center" valign="top" charoff="50">69 (2)</td></tr><tr><td align="left" valign="top" charoff="50">Meristematic cortical cells</td><td align="center" valign="top" charoff="50">6540 (80)</td><td align="center" valign="top" charoff="50">1420 (230)</td><td align="center" valign="top" charoff="50">290 (50)</td><td align="center" valign="top" charoff="50">1220 (50)</td><td align="center" valign="top" charoff="50">160 (25)</td><td align="center" valign="top" charoff="50">22530 (180)</td><td align="center" valign="top" charoff="50">5550 (70)</td><td align="center" valign="top" charoff="50">9 (3)</td><td align="center" valign="top" charoff="50"><11</td><td align="center" valign="top" charoff="50"><15</td><td align="center" valign="top" charoff="50"><27</td><td align="center" valign="top" charoff="50">76 (10)</td></tr><tr><td align="left" valign="top" charoff="50">Phloem</td><td align="center" valign="top" charoff="50">6430 (40)</td><td align="center" valign="top" charoff="50">1400 (340)</td><td align="center" valign="top" charoff="50">1600 (200)</td><td align="center" valign="top" charoff="50">1230 (60)</td><td align="center" valign="top" charoff="50">330 (18)</td><td align="center" valign="top" charoff="50">11550 (230)</td><td align="center" valign="top" charoff="50">8300 (120)</td><td align="center" valign="top" charoff="50">10 (3)</td><td align="center" valign="top" charoff="50">38 (5)</td><td align="center" valign="top" charoff="50"><9</td><td align="center" valign="top" charoff="50"><17</td><td align="center" valign="top" charoff="50">70 (9)</td></tr><tr><td align="left" valign="top" charoff="50">Xylem</td><td align="center" valign="top" charoff="50">367 (15)</td><td align="center" valign="top" charoff="50">1130 (230)</td><td align="center" valign="top" charoff="50">2000 (190)</td><td align="center" valign="top" charoff="50">650 (30)</td><td align="center" valign="top" charoff="50">150 (16)</td><td align="center" valign="top" charoff="50">4440 (60)</td><td align="center" valign="top" charoff="50">690 (16)</td><td align="center" valign="top" charoff="50">4.4 (0.5)</td><td align="center" valign="top" charoff="50">8.8 (0.7)</td><td align="center" valign="top" charoff="50"><1</td><td align="center" valign="top" charoff="50">18 (1)</td><td align="center" valign="top" charoff="50">10.4 (1)</td></tr><tr><td align="left" valign="top" charoff="50">Pith</td><td align="center" valign="top" charoff="50">2820 (40)</td><td align="center" valign="top" charoff="50">1810 (400)</td><td align="center" valign="top" charoff="50">4210 (420)</td><td align="center" valign="top" charoff="50">810 (40)</td><td align="center" valign="top" charoff="50">126 (19)</td><td align="center" valign="top" charoff="50">17500 (220)</td><td align="center" valign="top" charoff="50">6230 (80)</td><td align="center" valign="top" charoff="50">14 (1)</td><td align="center" valign="top" charoff="50">8 (1)</td><td align="center" valign="top" charoff="50"><4</td><td align="center" valign="top" charoff="50">21 (3)</td><td align="center" valign="top" charoff="50">62 (3)</td></tr></tbody></table><table-wrap-foot><fn id="t5-fn1"><p>The results were obtained from PIXE spectra extracted from regions representing different morphological structures, and analysed using GeoPIXE software. Errors of analysis (±1 σ uncertainty) are shown in brackets.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t6"><label>Table 6</label><caption><title>Elemental concentrations (micro-PIXE, μg g<sup>−1</sup> dry weight) in the morphological structures of the leaves of <italic>Phyllanthus balgooyi, Phyllanthus securinegioides</italic> and <italic>Rinorea bengalensis</italic>.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50"> </th><th align="center" valign="top" charoff="50">Ni</th><th align="center" valign="top" charoff="50">P</th><th align="center" valign="top" charoff="50">S</th><th align="center" valign="top" charoff="50">Cl</th><th align="center" valign="top" charoff="50">K</th><th align="center" valign="top" charoff="50">Ca</th><th align="center" valign="top" charoff="50">Mn</th><th align="center" valign="top" charoff="50">Fe</th></tr></thead><tbody valign="top"><tr><td colspan="9" align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td></tr><tr><td align="left" valign="top" charoff="50"> Whole area</td><td align="center" valign="top" charoff="50">3050 (50)</td><td align="center" valign="top" charoff="50">520 (40)</td><td align="center" valign="top" charoff="50">1100 (40)</td><td align="center" valign="top" charoff="50">6430 (60)</td><td align="center" valign="top" charoff="50">2160 (30)</td><td align="center" valign="top" charoff="50">16330 (130)</td><td align="center" valign="top" charoff="50">830 (15)</td><td align="center" valign="top" charoff="50">38 (3)</td></tr><tr><td align="left" valign="top" charoff="50"> Upper epidermis</td><td align="center" valign="top" charoff="50">9000 (130)</td><td align="center" valign="top" charoff="50"><130</td><td align="center" valign="top" charoff="50">410 (40)</td><td align="center" valign="top" charoff="50">2130 (130)</td><td align="center" valign="top" charoff="50">1710 (30)</td><td align="center" valign="top" charoff="50">33930 (270)</td><td align="center" valign="top" charoff="50">150 (10)</td><td align="center" valign="top" charoff="50">32 (8)</td></tr><tr><td align="left" valign="top" charoff="50"> Palisade mesophyll</td><td align="center" valign="top" charoff="50">160 (13)</td><td align="center" valign="top" charoff="50">590 (60)</td><td align="center" valign="top" charoff="50">850 (70)</td><td align="center" valign="top" charoff="50">10540 (90)</td><td align="center" valign="top" charoff="50">1630 (30)</td><td align="center" valign="top" charoff="50">3050 (40)</td><td align="center" valign="top" charoff="50">1910 (40)</td><td align="center" valign="top" charoff="50">27 (11)</td></tr><tr><td align="left" valign="top" charoff="50"> Xylem</td><td align="center" valign="top" charoff="50">200 (40)</td><td align="center" valign="top" charoff="50"><244</td><td align="center" valign="top" charoff="50">300 (90)</td><td align="center" valign="top" charoff="50">4580 (130)</td><td align="center" valign="top" charoff="50">2250 (80)</td><td align="center" valign="top" charoff="50">1960 (80)</td><td align="center" valign="top" charoff="50">390 (50)</td><td align="center" valign="top" charoff="50">43 (28)</td></tr><tr><td align="left" valign="top" charoff="50"> Phloem</td><td align="center" valign="top" charoff="50">29400 (350)</td><td align="center" valign="top" charoff="50">680 (80)</td><td align="center" valign="top" charoff="50">1140 (70)</td><td align="center" valign="top" charoff="50">9730 (120)</td><td align="center" valign="top" charoff="50">2190 (40)</td><td align="center" valign="top" charoff="50">3100 (50)</td><td align="center" valign="top" charoff="50">2700 (60)</td><td align="center" valign="top" charoff="50"><120</td></tr><tr><td align="left" valign="top" charoff="50"> Spongy mesophyll</td><td align="center" valign="top" charoff="50">1210 (45)</td><td align="center" valign="top" charoff="50">790 (60)</td><td align="center" valign="top" charoff="50">1900 (40)</td><td align="center" valign="top" charoff="50">5250 (30)</td><td align="center" valign="top" charoff="50">3300 (50)</td><td align="center" valign="top" charoff="50">37700 (210)</td><td align="center" valign="top" charoff="50">230 (9)</td><td align="center" valign="top" charoff="50">51 (10)</td></tr><tr><td align="left" valign="top" charoff="50"> Lower epidermis</td><td align="center" valign="top" charoff="50">1930 (60)</td><td align="center" valign="top" charoff="50">490 (60)</td><td align="center" valign="top" charoff="50">960 (50)</td><td align="center" valign="top" charoff="50">7700 (60)</td><td align="center" valign="top" charoff="50">1340 (30)</td><td align="center" valign="top" charoff="50">17460 (150)</td><td align="center" valign="top" charoff="50">480 (17)</td><td align="center" valign="top" charoff="50">29 (10)</td></tr><tr><td colspan="9" align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic><hr></hr></td></tr><tr><td align="left" valign="top" charoff="50"> Whole area</td><td align="center" valign="top" charoff="50">9490 (150)</td><td align="center" valign="top" charoff="50">500 (30)</td><td align="center" valign="top" charoff="50">1120 (70)</td><td align="center" valign="top" charoff="50">16070 (200)</td><td align="center" valign="top" charoff="50">9000 (140)</td><td align="center" valign="top" charoff="50">2300 (60)</td><td align="center" valign="top" charoff="50">41 (5)</td><td align="center" valign="top" charoff="50">16 (3)</td></tr><tr><td align="left" valign="top" charoff="50"> Upper epidermis</td><td align="center" valign="top" charoff="50">39500 (600)</td><td align="center" valign="top" charoff="50"><470</td><td align="center" valign="top" charoff="50">1630 (90)</td><td align="center" valign="top" charoff="50">16840 (170)</td><td align="center" valign="top" charoff="50">3340 (70)</td><td align="center" valign="top" charoff="50">1780 (40)</td><td align="center" valign="top" charoff="50">480 (28)</td><td align="center" valign="top" charoff="50">85 (18)</td></tr><tr><td align="left" valign="top" charoff="50"> Palisade mesophyll</td><td align="center" valign="top" charoff="50">1700 (50)</td><td align="center" valign="top" charoff="50">1450 (100)</td><td align="center" valign="top" charoff="50">2240 (80)</td><td align="center" valign="top" charoff="50">7700 (90)</td><td align="center" valign="top" charoff="50">9470 (70)</td><td align="center" valign="top" charoff="50">2910 (60)</td><td align="center" valign="top" charoff="50">21 (8)</td><td align="center" valign="top" charoff="50">35 (9)</td></tr><tr><td align="left" valign="top" charoff="50"> Xylem</td><td align="center" valign="top" charoff="50">650 (19)</td><td align="center" valign="top" charoff="50">750 (40)</td><td align="center" valign="top" charoff="50">450 (30)</td><td align="center" valign="top" charoff="50">6240 (60)</td><td align="center" valign="top" charoff="50">9400 (120)</td><td align="center" valign="top" charoff="50">330 (40)</td><td align="center" valign="top" charoff="50">5 (3)</td><td align="center" valign="top" charoff="50"><3</td></tr><tr><td align="left" valign="top" charoff="50"> Phloem</td><td align="center" valign="top" charoff="50">8200 (150)</td><td align="center" valign="top" charoff="50">420 (80)</td><td align="center" valign="top" charoff="50">640 (70)</td><td align="center" valign="top" charoff="50">11510 (140)</td><td align="center" valign="top" charoff="50">14400 (100)</td><td align="center" valign="top" charoff="50">9500 (80)</td><td align="center" valign="top" charoff="50"><11</td><td align="center" valign="top" charoff="50"><12</td></tr><tr><td align="left" valign="top" charoff="50"> Spongy mesophyll</td><td align="center" valign="top" charoff="50">4820 (110)</td><td align="center" valign="top" charoff="50"><360</td><td align="center" valign="top" charoff="50">630 (70)</td><td align="center" valign="top" charoff="50">14460 (260)</td><td align="center" valign="top" charoff="50">10040 (130)</td><td align="center" valign="top" charoff="50">970 (50)</td><td align="center" valign="top" charoff="50"><22</td><td align="center" valign="top" charoff="50"><23</td></tr><tr><td align="left" valign="top" charoff="50"> Lower epidermis</td><td align="center" valign="top" charoff="50">19010 (200)</td><td align="center" valign="top" charoff="50">490 (100)</td><td align="center" valign="top" charoff="50">1130 (70)</td><td align="center" valign="top" charoff="50">27800 (200)</td><td align="center" valign="top" charoff="50">6200 (70)</td><td align="center" valign="top" charoff="50">3840 (50)</td><td align="center" valign="top" charoff="50"><32</td><td align="center" valign="top" charoff="50"><36</td></tr><tr><td colspan="9" align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic><hr></hr></td></tr><tr><td align="left" valign="top" charoff="50"> Whole area</td><td align="center" valign="top" charoff="50">7100 (100)</td><td align="center" valign="top" charoff="50">1030 (60)</td><td align="center" valign="top" charoff="50">2980 (100)</td><td align="center" valign="top" charoff="50">3740 (70)</td><td align="center" valign="top" charoff="50">12030 (90)</td><td align="center" valign="top" charoff="50">4870 (50)</td><td align="center" valign="top" charoff="50">110 (4)</td><td align="center" valign="top" charoff="50">36 (3)</td></tr><tr><td align="left" valign="top" charoff="50"> Upper epidermis</td><td align="center" valign="top" charoff="50">11800 (140)</td><td align="center" valign="top" charoff="50">460 (40)</td><td align="center" valign="top" charoff="50">5110 (90)</td><td align="center" valign="top" charoff="50">4770 (40)</td><td align="center" valign="top" charoff="50">7420 (60)</td><td align="center" valign="top" charoff="50">33530 (40)</td><td align="center" valign="top" charoff="50">400 (14)</td><td align="center" valign="top" charoff="50">20 (5)</td></tr><tr><td align="left" valign="top" charoff="50"> Palisade mesophyll</td><td align="center" valign="top" charoff="50">9210 (110)</td><td align="center" valign="top" charoff="50">860 (60)</td><td align="center" valign="top" charoff="50">5690 (120)</td><td align="center" valign="top" charoff="50">6130 (50)</td><td align="center" valign="top" charoff="50">13300 (100)</td><td align="center" valign="top" charoff="50">3970 (40)</td><td align="center" valign="top" charoff="50">300 (9)</td><td align="center" valign="top" charoff="50">23 (10)</td></tr><tr><td align="left" valign="top" charoff="50"> Xylem</td><td align="center" valign="top" charoff="50">1100 (30)</td><td align="center" valign="top" charoff="50">980 (110)</td><td align="center" valign="top" charoff="50">1710 (90)</td><td align="center" valign="top" charoff="50">3590 (60)</td><td align="center" valign="top" charoff="50">21090 (110)</td><td align="center" valign="top" charoff="50">2060 (60)</td><td align="center" valign="top" charoff="50">15 (7)</td><td align="center" valign="top" charoff="50">19 (8)</td></tr><tr><td align="left" valign="top" charoff="50"> Phloem</td><td align="center" valign="top" charoff="50">1170 (40)</td><td align="center" valign="top" charoff="50">1430 (110)</td><td align="center" valign="top" charoff="50">1800 (100)</td><td align="center" valign="top" charoff="50">3740 (60)</td><td align="center" valign="top" charoff="50">24050 (150)</td><td align="center" valign="top" charoff="50">2830 (110)</td><td align="center" valign="top" charoff="50"><14</td><td align="center" valign="top" charoff="50">19 (8)</td></tr><tr><td align="left" valign="top" charoff="50"> Spongy mesophyll</td><td align="center" valign="top" charoff="50">17800 (190)</td><td align="center" valign="top" charoff="50">1600 (100)</td><td align="center" valign="top" charoff="50">2800 (80)</td><td align="center" valign="top" charoff="50">3850 (43)</td><td align="center" valign="top" charoff="50">13680 (70)</td><td align="center" valign="top" charoff="50">4060 (50)</td><td align="center" valign="top" charoff="50">56 (4)</td><td align="center" valign="top" charoff="50">14 (5)</td></tr><tr><td align="left" valign="top" charoff="50"> Lower epidermis</td><td align="center" valign="top" charoff="50">13700 (150)</td><td align="center" valign="top" charoff="50">240 (24)</td><td align="center" valign="top" charoff="50">1930 (40)</td><td align="center" valign="top" charoff="50">2310 (43)</td><td align="center" valign="top" charoff="50">4480 (35)</td><td align="center" valign="top" charoff="50">1090 (30)</td><td align="center" valign="top" charoff="50">40 (3)</td><td align="center" valign="top" charoff="50">59 (4)</td></tr></tbody></table><table-wrap-foot><fn id="t6-fn1"><p>The results were obtained from PIXE spectra extracted from regions representing different morphological structures, and analysed using GeoPIXE software. Errors of analysis (±1 σ uncertainty) are shown in brackets.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t7"><label>Table 7</label><caption><title>Major metabolites and identified Ni-complexes in leaf and phloem extracts of <italic>P. balgooyi, P. securinegioides</italic> and <italic>R. bengalensis</italic>.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th rowspan="3" align="center" valign="top" charoff="50">Metabolites</th><th colspan="4" align="center" valign="top" charoff="50">Plant tissue and phloem sap samples<hr></hr></th></tr><tr><th align="center" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic> (phloem tissue)</th><th align="center" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic> (phloem sap)</th><th align="center" valign="top" charoff="50"><italic>Rinorea bengalensis</italic> (phloem tissue)</th><th align="center" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic> (phloem tissue)</th></tr><tr><th align="center" valign="top" charoff="50">Mean (n = 3)</th><th align="center" valign="top" charoff="50">Mean (n = 3)</th><th align="center" valign="top" charoff="50">Mean (n = 3)</th><th align="center" valign="top" charoff="50">Mean (n = 3)</th></tr></thead><tbody valign="top"><tr><td colspan="5" align="center" valign="top" charoff="50"><italic><bold>Relative Response Ratios</bold> ( <bold>RRR’s</bold></italic>)</td></tr><tr><td align="left" valign="top" charoff="50">Pyruvic acid</td><td align="center" valign="top" charoff="50">0.0372 ± 0.0108</td><td align="center" valign="top" charoff="50">0.0417 ± 0.0139</td><td align="center" valign="top" charoff="50">0.0154 ± 0.0041</td><td align="center" valign="top" charoff="50">0.0096 ± 0.0049</td></tr><tr><td align="left" valign="top" charoff="50">Lactate</td><td align="center" valign="top" charoff="50">0.0399 ± 0.0093</td><td align="center" valign="top" charoff="50">0.0126 ± 0.0139</td><td align="center" valign="top" charoff="50">0.0615 ± 0.0147</td><td align="center" valign="top" charoff="50">0.0483 ± 0.0201</td></tr><tr><td align="left" valign="top" charoff="50">Glycolic acid</td><td align="center" valign="top" charoff="50">0.0170 ± 0.0017</td><td align="center" valign="top" charoff="50">0.0151 ± 0.0167</td><td align="center" valign="top" charoff="50">0.0096 ± 0.0010</td><td align="center" valign="top" charoff="50">0.0149 ± 0.0002</td></tr><tr><td align="left" valign="top" charoff="50">Alanine 2TMS</td><td align="center" valign="top" charoff="50">0.0070 ± 0.0010</td><td align="center" valign="top" charoff="50">0.0012 ± 0.0015</td><td align="center" valign="top" charoff="50">0.0261 ± 0.0021</td><td align="center" valign="top" charoff="50">0.0098 ± 0.0016</td></tr><tr><td align="left" valign="top" charoff="50">Glycine</td><td align="center" valign="top" charoff="50">0.0008 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0005 ± 0.0006</td><td align="center" valign="top" charoff="50">0.0051 ± 0.0008</td><td align="center" valign="top" charoff="50">0.0010 ± 0.0001</td></tr><tr><td align="left" valign="top" charoff="50">butyro-1,4-lactam 1TMS</td><td align="center" valign="top" charoff="50">0.0130 ± 0.0003</td><td align="center" valign="top" charoff="50">0.0017 ± 0.0035</td><td align="center" valign="top" charoff="50">0.0466 ± 0.0084</td><td align="center" valign="top" charoff="50">0.0174 ± 0.0026</td></tr><tr><td align="left" valign="top" charoff="50">Valine</td><td align="center" valign="top" charoff="50">0.0105 ± 0.0015</td><td align="center" valign="top" charoff="50">0.0037 ± 0.0063</td><td align="center" valign="top" charoff="50">0.0331 ± 0.0038</td><td align="center" valign="top" charoff="50">0.0174 ± 0.0013</td></tr><tr><td align="left" valign="top" charoff="50">Benzoate</td><td align="center" valign="top" charoff="50">0.0615 ± 0.0039</td><td align="center" valign="top" charoff="50">0.0438 ± 0.0537</td><td align="center" valign="top" charoff="50">0.0273 ± 0.0021</td><td align="center" valign="top" charoff="50">0.0456 ± 0.0019</td></tr><tr><td align="left" valign="top" charoff="50">Serine</td><td align="center" valign="top" charoff="50">0.0050 ± 0.0009</td><td align="center" valign="top" charoff="50">0.0003 ± 0.0005</td><td align="center" valign="top" charoff="50">0.0239 ± 0.0028</td><td align="center" valign="top" charoff="50">0.0045 ± 0.0011</td></tr><tr><td align="left" valign="top" charoff="50">Glycerol/Phosphate</td><td align="center" valign="top" charoff="50">0.3294 ± 0.1643</td><td align="center" valign="top" charoff="50">0.0292 ± 0.0698</td><td align="center" valign="top" charoff="50">0.9054 ± 0.0902</td><td align="center" valign="top" charoff="50">0.4143 ± 0.0671</td></tr><tr><td align="left" valign="top" charoff="50">Isoleucine</td><td align="center" valign="top" charoff="50">0.0030 ± 0.0009</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0197 ± 0.0020</td><td align="center" valign="top" charoff="50">0.0056 ± 0.0009</td></tr><tr><td align="left" valign="top" charoff="50">Nicotinic acid</td><td align="center" valign="top" charoff="50">0.0266 ± 0.0008</td><td align="center" valign="top" charoff="50">0.0086 ± 0.0109</td><td align="center" valign="top" charoff="50">0.0254 ± 0.0019</td><td align="center" valign="top" charoff="50">0.0125 ± 0.0014</td></tr><tr><td align="left" valign="top" charoff="50">Glycine</td><td align="center" valign="top" charoff="50">0.0015 ± 0.0005</td><td align="center" valign="top" charoff="50">0.0004 ± 0.0004</td><td align="center" valign="top" charoff="50">0.0083 ± 0.0005</td><td align="center" valign="top" charoff="50">0.0018 ± 0.0005</td></tr><tr><td align="left" valign="top" charoff="50">Succinate</td><td align="center" valign="top" charoff="50">0.0115 ± 0.0008</td><td align="center" valign="top" charoff="50">0.0038 ± 0.0045</td><td align="center" valign="top" charoff="50">0.0310 ± 0.0038</td><td align="center" valign="top" charoff="50">0.0309 ± 0.0059</td></tr><tr><td align="left" valign="top" charoff="50">Glyceric acid</td><td align="center" valign="top" charoff="50">0.0042 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0007 ± 0.0008</td><td align="center" valign="top" charoff="50">0.0131 ± 0.0024</td><td align="center" valign="top" charoff="50">0.0037 ± 0.0004</td></tr><tr><td align="left" valign="top" charoff="50">Uracil</td><td align="center" valign="top" charoff="50">0.0297 ± 0.0045</td><td align="center" valign="top" charoff="50">0.0122 ± 0.0166</td><td align="center" valign="top" charoff="50">0.0173 ± 0.0020</td><td align="center" valign="top" charoff="50">0.0211 ± 0.0015</td></tr><tr><td align="left" valign="top" charoff="50">Itaconic acid</td><td align="center" valign="top" charoff="50">0.0247 ± 0.0027</td><td align="center" valign="top" charoff="50">0.0169 ± 0.0143</td><td align="center" valign="top" charoff="50">0.0219 ± 0.0002</td><td align="center" valign="top" charoff="50">0.0156 ± 0.0048</td></tr><tr><td align="left" valign="top" charoff="50">Fumarate</td><td align="center" valign="top" charoff="50">0.0216 ± 0.0021</td><td align="center" valign="top" charoff="50">0.0053 ± 0.0078</td><td align="center" valign="top" charoff="50">0.0490 ± 0.0050</td><td align="center" valign="top" charoff="50">0.0235 ± 0.0044</td></tr><tr><td align="left" valign="top" charoff="50">Serine</td><td align="center" valign="top" charoff="50">0.0132 ± 0.0007</td><td align="center" valign="top" charoff="50">0.0016 ± 0.0023</td><td align="center" valign="top" charoff="50">0.0848 ± 0.0091</td><td align="center" valign="top" charoff="50">0.0132 ± 0.0018</td></tr><tr><td align="left" valign="top" charoff="50">3-cyano-alanine</td><td align="center" valign="top" charoff="50">0.0006 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0005 ± 0.0008</td><td align="center" valign="top" charoff="50">0.0007 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0009 ± 0.0001</td></tr><tr><td align="left" valign="top" charoff="50">Threonine</td><td align="center" valign="top" charoff="50">0.0079 ± 0.0014</td><td align="center" valign="top" charoff="50">0.0011 ± 0.0019</td><td align="center" valign="top" charoff="50">0.0223 ± 0.0020</td><td align="center" valign="top" charoff="50">0.0085 ± 0.0005</td></tr><tr><td align="left" valign="top" charoff="50">2-methyl maleic acid</td><td align="center" valign="top" charoff="50">0.0101 ± 0.0007</td><td align="center" valign="top" charoff="50">0.0072 ± 0.0072</td><td align="center" valign="top" charoff="50">0.0097 ± 0.0010</td><td align="center" valign="top" charoff="50">0.0108 ± 0.0029</td></tr><tr><td align="left" valign="top" charoff="50">Aspartate</td><td align="center" valign="top" charoff="50">0.0052 ± 0.0019</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0005</td><td align="center" valign="top" charoff="50">0.0081 ± 0.0004</td><td align="center" valign="top" charoff="50">0.0011 ± 0.0003</td></tr><tr><td align="left" valign="top" charoff="50">Glutamine</td><td align="center" valign="top" charoff="50">0.0009 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0001 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0058 ± 0.0009</td><td align="center" valign="top" charoff="50">0.0006 ± 0.0002</td></tr><tr><td align="left" valign="top" charoff="50">Malate</td><td align="center" valign="top" charoff="50">0.1190 ± 0.0168</td><td align="center" valign="top" charoff="50">0.0132 ± 0.0130</td><td align="center" valign="top" charoff="50">0.8612 ± 0.0925</td><td align="center" valign="top" charoff="50">0.3969 ± 0.0923</td></tr><tr><td align="left" valign="top" charoff="50">Erythritol</td><td align="center" valign="top" charoff="50">0.0022 ± 0.0009</td><td align="center" valign="top" charoff="50">0.0002 ± 0.0007</td><td align="center" valign="top" charoff="50">0.0101 ± 0.0025</td><td align="center" valign="top" charoff="50">0.0037 ± 0.0007</td></tr><tr><td align="left" valign="top" charoff="50">Aspartate</td><td align="center" valign="top" charoff="50">0.2400 ± 0.0841</td><td align="center" valign="top" charoff="50">0.0063 ± 0.0265</td><td align="center" valign="top" charoff="50">1.1451 ± 0.0441</td><td align="center" valign="top" charoff="50">0.0748 ± 0.0297</td></tr><tr><td align="left" valign="top" charoff="50">Pyroglutamate</td><td align="center" valign="top" charoff="50">0.3796 ± 0.0432</td><td align="center" valign="top" charoff="50">0.0706 ± 0.1181</td><td align="center" valign="top" charoff="50">1.0969 ± 0.0553</td><td align="center" valign="top" charoff="50">0.5092 ± 0.1199</td></tr><tr><td align="left" valign="top" charoff="50">4-amino-butyric acid</td><td align="center" valign="top" charoff="50">0.0477 ± 0.0070</td><td align="center" valign="top" charoff="50">0.0009 ± 0.0009</td><td align="center" valign="top" charoff="50">0.1910 ± 0.0102</td><td align="center" valign="top" charoff="50">0.1033 ± 0.0054</td></tr><tr><td align="left" valign="top" charoff="50">Glutamate</td><td align="center" valign="top" charoff="50">0.0252 ± 0.0057</td><td align="center" valign="top" charoff="50">0.0024 ± 0.0040</td><td align="center" valign="top" charoff="50">0.1892 ± 0.0169</td><td align="center" valign="top" charoff="50">0.0943 ± 0.0217</td></tr><tr><td align="left" valign="top" charoff="50">4-hydroxy-benozic acid</td><td align="center" valign="top" charoff="50">0.2051 ± 0.0177</td><td align="center" valign="top" charoff="50">0.0933 ± 0.1161</td><td align="center" valign="top" charoff="50">0.0787 ± 0.0038</td><td align="center" valign="top" charoff="50">0.1048 ± 0.0056</td></tr><tr><td align="left" valign="top" charoff="50">Xylose</td><td align="center" valign="top" charoff="50">0.0010 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0194 ± 0.0032</td><td align="center" valign="top" charoff="50">0.0010 ± 0.0002</td></tr><tr><td align="left" valign="top" charoff="50">1,6-anhydroglucose</td><td align="center" valign="top" charoff="50">0.0059 ± 0.0007</td><td align="center" valign="top" charoff="50">0.0036 ± 0.0070</td><td align="center" valign="top" charoff="50">0.0093 ± 0.0006</td><td align="center" valign="top" charoff="50">0.0116 ± 0.0017</td></tr><tr><td align="left" valign="top" charoff="50">Xylitol</td><td align="center" valign="top" charoff="50">0.0095 ± 0.0013</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0070 ± 0.0006</td><td align="center" valign="top" charoff="50">0.0094 ± 0.0016</td></tr><tr><td align="left" valign="top" charoff="50">cis-aconitic acid</td><td align="center" valign="top" charoff="50">0.0797 ± 0.0135</td><td align="center" valign="top" charoff="50">0.0202 ± 0.0235</td><td align="center" valign="top" charoff="50">0.0497 ± 0.0017</td><td align="center" valign="top" charoff="50">0.0104 ± 0.0035</td></tr><tr><td align="left" valign="top" charoff="50">Glycerol-3-phosphate</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0007 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0012 ± 0.0001</td></tr><tr><td align="left" valign="top" charoff="50">4-hydroxy-3-methoxybenzoiac acid</td><td align="center" valign="top" charoff="50">0.1297 ± 0.0797</td><td align="center" valign="top" charoff="50">0.0104 ± 0.0214</td><td align="center" valign="top" charoff="50">0.0709 ± 0.0072</td><td align="center" valign="top" charoff="50">0.0609 ± 0.0173</td></tr><tr><td align="left" valign="top" charoff="50">Pinitol</td><td align="center" valign="top" charoff="50">0.0002 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0001 ± 0.0002</td><td align="center" valign="top" charoff="50">0.7277 ± 0.0526</td><td align="center" valign="top" charoff="50">0.0005 ± 0.0001</td></tr><tr><td align="left" valign="top" charoff="50">Myoinositol</td><td align="center" valign="top" charoff="50">0.0004 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0002 ± 0.0002</td><td align="center" valign="top" charoff="50">0.4952 ± 0.0532</td><td align="center" valign="top" charoff="50">0.0011 ± 0.0001</td></tr><tr><td align="left" valign="top" charoff="50">Galactonic acid</td><td align="center" valign="top" charoff="50">0.0025 ± 0.0008</td><td align="center" valign="top" charoff="50">0.0004 ± 0.0003</td><td align="center" valign="top" charoff="50">0.0226 ± 0.0038</td><td align="center" valign="top" charoff="50">0.0494 ± 0.0092</td></tr><tr><td align="left" valign="top" charoff="50">Saccharic acid</td><td align="center" valign="top" charoff="50">0.0370 ± 0.0126</td><td align="center" valign="top" charoff="50">0.0064 ± 0.0062</td><td align="center" valign="top" charoff="50">0.5932 ± 0.0649</td><td align="center" valign="top" charoff="50">0.0030 ± 0.0012</td></tr><tr><td align="left" valign="top" charoff="50">Mucic acid</td><td align="center" valign="top" charoff="50">0.0126 ± 0.0039</td><td align="center" valign="top" charoff="50">0.0035 ± 0.0092</td><td align="center" valign="top" charoff="50">0.4892 ± 0.0547</td><td align="center" valign="top" charoff="50">0.0032 ± 0.0004</td></tr><tr><td align="left" valign="top" charoff="50">Hexadecanoic acid</td><td align="center" valign="top" charoff="50">0.3215 ± 0.0460</td><td align="center" valign="top" charoff="50">0.1719 ± 0.2136</td><td align="center" valign="top" charoff="50">0.1369 ± 0.0052</td><td align="center" valign="top" charoff="50">0.1817 ± 0.0091</td></tr><tr><td align="left" valign="top" charoff="50">Inositol</td><td align="center" valign="top" charoff="50">0.1708 ± 0.0269</td><td align="center" valign="top" charoff="50">0.0052 ± 0.0046</td><td align="center" valign="top" charoff="50">0.7108 ± 0.0829</td><td align="center" valign="top" charoff="50">0.6813 ± 0.0564</td></tr><tr><td align="left" valign="top" charoff="50">3-deoxy-arabino-hexaric acid</td><td align="center" valign="top" charoff="50">0.0497 ± 0.0037</td><td align="center" valign="top" charoff="50">0.0191 ± 0.0271</td><td align="center" valign="top" charoff="50">0.1238 ± 0.0191</td><td align="center" valign="top" charoff="50">0.0103 ± 0.0015</td></tr><tr><td align="left" valign="top" charoff="50">Galactose</td><td align="center" valign="top" charoff="50">0.0013 ± 0.0003</td><td align="center" valign="top" charoff="50">0.0011 ± 0.0007</td><td align="center" valign="top" charoff="50">0.0727 ± 0.0064</td><td align="center" valign="top" charoff="50">0.0315 ± 0.0009</td></tr><tr><td align="left" valign="top" charoff="50">Maltitol</td><td align="center" valign="top" charoff="50">0.0011 ± 0.0005</td><td align="center" valign="top" charoff="50">0.0001 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0019 ± 0.0016</td><td align="center" valign="top" charoff="50">0.0003 ± 0.0000</td></tr><tr><td align="left" valign="top" charoff="50">Fructose-6-phosphate</td><td align="center" valign="top" charoff="50">0.0007 ± 0.0002</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0129 ± 0.0023</td><td align="center" valign="top" charoff="50">0.0029 ± 0.0005</td></tr><tr><td align="left" valign="top" charoff="50">Glucose-6-phosphate</td><td align="center" valign="top" charoff="50">0.0013 ± 0.0004</td><td align="center" valign="top" charoff="50">0.0000 ± 0.0000</td><td align="center" valign="top" charoff="50">0.0404 ± 0.0083</td><td align="center" valign="top" charoff="50">0.0074 ± 0.0007</td></tr><tr><td align="left" valign="top" charoff="50">Trehalose</td><td align="center" valign="top" charoff="50">0.3398 ± 0.3342</td><td align="center" valign="top" charoff="50">0.0003 ± 0.0042</td><td align="center" valign="top" charoff="50">0.3895 ± 0.0051</td><td align="center" valign="top" charoff="50">0.0443 ± 0.0106</td></tr><tr><td align="left" valign="top" charoff="50">Maltose</td><td align="center" valign="top" charoff="50">0.0067 ± 0.0029</td><td align="center" valign="top" charoff="50">0.0004 ± 0.0006</td><td align="center" valign="top" charoff="50">0.0161 ± 0.0046</td><td align="center" valign="top" charoff="50">0.0338 ± 0.0031</td></tr><tr><td align="left" valign="top" charoff="50">Gentobiose</td><td align="center" valign="top" charoff="50">0.0093 ± 0.0021</td><td align="center" valign="top" charoff="50">0.0001 ± 0.0003</td><td align="center" valign="top" charoff="50">0.0418 ± 0.0076</td><td align="center" valign="top" charoff="50">0.0062 ± 0.0005</td></tr><tr><td align="left" valign="top" charoff="50">Catechin</td><td align="center" valign="top" charoff="50">4.4191 ± 0.8557</td><td align="center" valign="top" charoff="50">0.0029 ± 0.0219</td><td align="center" valign="top" charoff="50">0.0140 ± 0.0047</td><td align="center" valign="top" charoff="50">1.3337 ± 0.3064</td></tr><tr><td align="left" valign="top" charoff="50">Melibiose</td><td align="center" valign="top" charoff="50">0.0015 ± 0.0003</td><td align="center" valign="top" charoff="50">0.0001 ± 0.0001</td><td align="center" valign="top" charoff="50">0.0191 ± 0.0016</td><td align="center" valign="top" charoff="50">0.0056 ± 0.0018</td></tr><tr><td align="left" valign="top" charoff="50">Raffinose</td><td align="center" valign="top" charoff="50">0.1009 ± 0.0170</td><td align="center" valign="top" charoff="50">0.0016 ± 0.0021</td><td align="center" valign="top" charoff="50">0.0435 ± 0.0113</td><td align="center" valign="top" charoff="50">0.0732 ± 0.0108</td></tr><tr><td align="left" valign="top" charoff="50">Citrate*</td><td align="center" valign="top" charoff="50"><bold>40.0191 ± 2.1491</bold></td><td align="center" valign="top" charoff="50"><bold>6.1564 ± 8.4050</bold></td><td align="center" valign="top" charoff="50"><bold>13.4089 ± 0.6403</bold></td><td align="center" valign="top" charoff="50"><bold>2.2423 ± 0.1830</bold></td></tr><tr><td align="left" valign="top" charoff="50">Fructose</td><td align="center" valign="top" charoff="50">0.3981 ± 0.1534</td><td align="center" valign="top" charoff="50">0.0086 ± 0.0085</td><td align="center" valign="top" charoff="50">24.9293 ± 5.2262</td><td align="center" valign="top" charoff="50">0.4236 ± 0.0355</td></tr><tr><td align="left" valign="top" charoff="50">Glucose</td><td align="center" valign="top" charoff="50">0.8223 ± 0.1927</td><td align="center" valign="top" charoff="50">0.0110 ± 0.0091</td><td align="center" valign="top" charoff="50">46.2812 ± 10.4612</td><td align="center" valign="top" charoff="50">0.7154 ± 0.0456</td></tr><tr><td align="left" valign="top" charoff="50">Sucrose</td><td align="center" valign="top" charoff="50">6.3945 ± 2.4236</td><td align="center" valign="top" charoff="50">0.1041 ± 0.0605</td><td align="center" valign="top" charoff="50">32.8001 ± 0.6488</td><td align="center" valign="top" charoff="50">16.7075 ± 1.9097</td></tr></tbody></table><table-wrap-foot><fn id="t7-fn1"><p>Results obtained with GC-MS. Nickel complexes identified by LC-MS in bold and indicated by asterisk.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t8"><label>Table 8</label><caption><title>Concentrations of citric acid, malic acid, fructose, sucrose, glucose and catechin in leaf and phloem extracts of <italic>P. balgooyi, P. securinegioides</italic> and <italic>R. bengalensis</italic>.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50">Species</th><th align="center" valign="top" charoff="50">Part</th><th align="center" valign="top" charoff="50">Sample</th><th align="center" valign="top" charoff="50">Malate</th><th align="center" valign="top" charoff="50">Citrate</th><th align="center" valign="top" charoff="50">Fructose</th><th align="center" valign="top" charoff="50">Glucose</th><th align="center" valign="top" charoff="50">Sucrose</th><th align="center" valign="top" charoff="50">Catechin</th></tr><tr><th align="left" valign="top" charoff="50"> </th><th colspan="2" align="center" valign="top" charoff="50"> </th><th colspan="6" align="center" valign="top" charoff="50"><bold>μg g</bold><sup><bold>−1</bold></sup><bold>(dry weight)</bold></th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">0.8</td><td align="center" valign="top" charoff="50">121.7</td><td align="center" valign="top" charoff="50">0.4</td><td align="center" valign="top" charoff="50">0.5</td><td align="center" valign="top" charoff="50">13.9</td><td align="center" valign="top" charoff="50">5.9</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">0.5</td><td align="center" valign="top" charoff="50">81.6</td><td align="center" valign="top" charoff="50">0.4</td><td align="center" valign="top" charoff="50">0.4</td><td align="center" valign="top" charoff="50">10.4</td><td align="center" valign="top" charoff="50">3.9</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">0.5</td><td align="center" valign="top" charoff="50">102.5</td><td align="center" valign="top" charoff="50">1.6</td><td align="center" valign="top" charoff="50">1.1</td><td align="center" valign="top" charoff="50">62.4</td><td align="center" valign="top" charoff="50">3.2</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">Phloem sap</td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">12.8</td><td align="center" valign="top" charoff="50">480.0</td><td align="center" valign="top" charoff="50">2.3</td><td align="center" valign="top" charoff="50">2.7</td><td align="center" valign="top" charoff="50">4.9</td><td align="center" valign="top" charoff="50">n.d.</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">Phloem sap</td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">14.1</td><td align="center" valign="top" charoff="50">285.6</td><td align="center" valign="top" charoff="50">2.1</td><td align="center" valign="top" charoff="50">1.5</td><td align="center" valign="top" charoff="50">6.7</td><td align="center" valign="top" charoff="50">n.d.</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus balgooyi</italic></td><td align="center" valign="top" charoff="50">Phloem sap</td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">14.4</td><td align="center" valign="top" charoff="50">314.8</td><td align="center" valign="top" charoff="50">1.5</td><td align="center" valign="top" charoff="50">0.7</td><td align="center" valign="top" charoff="50">11.9</td><td align="center" valign="top" charoff="50">n.d.</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">1.1</td><td align="center" valign="top" charoff="50">12.1</td><td align="center" valign="top" charoff="50">0.5</td><td align="center" valign="top" charoff="50">0.4</td><td align="center" valign="top" charoff="50">40.7</td><td align="center" valign="top" charoff="50">1.7</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">1.4</td><td align="center" valign="top" charoff="50">14.0</td><td align="center" valign="top" charoff="50">0.4</td><td align="center" valign="top" charoff="50">0.4</td><td align="center" valign="top" charoff="50">43.1</td><td align="center" valign="top" charoff="50">1.7</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Phyllanthus securinegioides</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">1.2</td><td align="center" valign="top" charoff="50">14.9</td><td align="center" valign="top" charoff="50">0.6</td><td align="center" valign="top" charoff="50">0.5</td><td align="center" valign="top" charoff="50">50.9</td><td align="center" valign="top" charoff="50">1.9</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">3.8</td><td align="center" valign="top" charoff="50">70.9</td><td align="center" valign="top" charoff="50">19.4</td><td align="center" valign="top" charoff="50">16.2</td><td align="center" valign="top" charoff="50">186.0</td><td align="center" valign="top" charoff="50">n.d.</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">3.0</td><td align="center" valign="top" charoff="50">34.4</td><td align="center" valign="top" charoff="50">18.1</td><td align="center" valign="top" charoff="50">13.9</td><td align="center" valign="top" charoff="50">141.5</td><td align="center" valign="top" charoff="50">n.d.</td></tr><tr><td align="left" valign="top" charoff="50"><italic>Rinorea bengalensis</italic></td><td align="center" valign="top" charoff="50">Phloem tissue</td><td align="center" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">3.6</td><td align="center" valign="top" charoff="50">41.7</td><td align="center" valign="top" charoff="50">17.7</td><td align="center" valign="top" charoff="50">15.5</td><td align="center" valign="top" charoff="50">135.2</td><td align="center" valign="top" charoff="50">n.d.</td></tr></tbody></table><table-wrap-foot><fn id="t8-fn1"><p>Results obtained with quantitative GC-MS.</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t9"><label>Table 9</label><caption><title>Mass-balance including major cations and anions in the phloem sap of <italic>P. balgooyi</italic>.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th colspan="8" align="center" valign="top" charoff="50">Mass Balance <italic>Phyllanthus balgooy</italic>i phloem sap<hr></hr></th></tr><tr><th rowspan="2" align="center" valign="top" charoff="50">Sample</th><th colspan="5" align="center" valign="top" charoff="50">Anions μg g<sup>−1</sup> (dry weight)<hr></hr></th><th rowspan="2" align="center" valign="top" charoff="50">Citrate wt%</th><th rowspan="2" align="center" valign="top" charoff="50"> </th></tr><tr><th align="center" valign="top" charoff="50">Cl<sup>−</sup></th><th align="center" valign="top" charoff="50">NO<sub>3</sub><sup>−</sup></th><th align="center" valign="top" charoff="50">PO<sub>4</sub><sup>3−</sup></th><th align="center" valign="top" charoff="50">SO<sub>4</sub><sup>2−</sup></th><th align="center" valign="top" charoff="50">Sum anions wt%</th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">1295</td><td align="center" valign="top" charoff="50">700</td><td align="center" valign="top" charoff="50">711</td><td align="center" valign="top" charoff="50">4167</td><td align="center" valign="top" charoff="50">0.69</td><td align="center" valign="top" charoff="50">81.3</td><td align="center" valign="top" charoff="50"> </td></tr><tr><td align="left" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">1280</td><td align="center" valign="top" charoff="50">863</td><td align="center" valign="top" charoff="50">1178</td><td align="center" valign="top" charoff="50">903</td><td align="center" valign="top" charoff="50">0.42</td><td align="center" valign="top" charoff="50">79.2</td><td align="center" valign="top" charoff="50"> </td></tr><tr><td align="left" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">1097</td><td align="center" valign="top" charoff="50">369</td><td align="center" valign="top" charoff="50">593</td><td align="center" valign="top" charoff="50">599</td><td align="center" valign="top" charoff="50">0.27</td><td align="center" valign="top" charoff="50">68.9</td><td align="center" valign="top" charoff="50"> </td></tr><tr><td align="left" valign="top" charoff="50"> </td><td colspan="5" align="center" valign="top" charoff="50"><bold>Cations</bold><bold>μg g</bold><sup><bold>−1</bold></sup><bold>(dry weight)</bold></td><td align="center" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50"> </td></tr><tr><td align="left" valign="top" charoff="50"><bold>Sample</bold></td><td align="center" valign="top" charoff="50"><bold>K</bold><sup><bold>+</bold></sup></td><td align="center" valign="top" charoff="50"><bold>Mg</bold><sup><bold>2+</bold></sup></td><td align="center" valign="top" charoff="50"><bold>Ni</bold><sup><bold>2+</bold></sup></td><td align="center" valign="top" charoff="50"><bold>Ca</bold><sup><bold>2+</bold></sup></td><td align="center" valign="top" charoff="50"><bold>Co</bold><sup><bold>2+</bold></sup></td><td align="center" valign="top" charoff="50"><bold>Sum cations wt%</bold></td><td align="center" valign="top" charoff="50"><bold>Total balance wt%</bold></td></tr><tr><td align="left" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">649</td><td align="center" valign="top" charoff="50">821</td><td align="center" valign="top" charoff="50">145560</td><td align="center" valign="top" charoff="50">1300</td><td align="center" valign="top" charoff="50">1869</td><td align="center" valign="top" charoff="50">15.1</td><td align="center" valign="top" charoff="50">96.4</td></tr><tr><td align="left" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">1211</td><td align="center" valign="top" charoff="50">810</td><td align="center" valign="top" charoff="50">168514</td><td align="center" valign="top" charoff="50">1871</td><td align="center" valign="top" charoff="50">1449</td><td align="center" valign="top" charoff="50">17.4</td><td align="center" valign="top" charoff="50">96.6</td></tr><tr><td align="left" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">1532</td><td align="center" valign="top" charoff="50">595</td><td align="center" valign="top" charoff="50">154944</td><td align="center" valign="top" charoff="50">1960</td><td align="center" valign="top" charoff="50">557</td><td align="center" valign="top" charoff="50">16.1</td><td align="center" valign="top" charoff="50">85.0</td></tr></tbody></table><table-wrap-foot><fn id="t9-fn1"><p>Results obtained with ICP-AES, High Performance Liquid Chromatography (HPLC) and ion chromatography.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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