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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative Analysis of the Microtubule Interaction of Rabies Virus P3 Protein: Roles in Immune Evasion and Pathogenesis</title><author><name sortKey="Brice, Aaron" sort="Brice, Aaron" uniqKey="Brice A" first="Aaron" last="Brice">Aaron Brice</name><affiliation><nlm:aff id="a1"><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne</institution>, Melbourne, Victoria, 3010,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Whelan, Donna R" sort="Whelan, Donna R" uniqKey="Whelan D" first="Donna R." last="Whelan">Donna R. Whelan</name><affiliation><nlm:aff id="a2"><institution>School of Chemistry, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name><affiliation><nlm:aff id="a3"><institution>Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences</institution>, Gifu University, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></nlm:aff></affiliation><affiliation><nlm:aff id="a4"><institution>The United Graduate School of Veterinary Sciences, Gifu University</institution>, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></nlm:aff></affiliation></author><author><name sortKey="Shimizu, Kenta" sort="Shimizu, Kenta" uniqKey="Shimizu K" first="Kenta" last="Shimizu">Kenta Shimizu</name><affiliation><nlm:aff id="a4"><institution>The United Graduate School of Veterinary Sciences, Gifu University</institution>, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></nlm:aff></affiliation></author><author><name sortKey="Wiltzer Bach, Linda" sort="Wiltzer Bach, Linda" uniqKey="Wiltzer Bach L" first="Linda" last="Wiltzer-Bach">Linda Wiltzer-Bach</name><affiliation><nlm:aff id="a5"><institution>Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="a6"><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Lo, Camden Y" sort="Lo, Camden Y" uniqKey="Lo C" first="Camden Y." last="Lo">Camden Y. Lo</name><affiliation><nlm:aff id="a7"><institution>Monash Micro Imaging</institution>, 27-31 Wright Street, Clayton, Victoria, 3168,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Blondel, Danielle" sort="Blondel, Danielle" uniqKey="Blondel D" first="Danielle" last="Blondel">Danielle Blondel</name><affiliation><nlm:aff id="a8"><institution>Unité de Virologie Moleculaire et Structurale</institution>, CNRS, UPR 3296, 91198 Gif sur Yvette Cedex,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Jans, David A" sort="Jans, David A" uniqKey="Jans D" first="David A." last="Jans">David A. Jans</name><affiliation><nlm:aff id="a5"><institution>Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Bell, Toby D M" sort="Bell, Toby D M" uniqKey="Bell T" first="Toby D. M." last="Bell">Toby D. M. Bell</name><affiliation><nlm:aff id="a2"><institution>School of Chemistry, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Moseley, Gregory W" sort="Moseley, Gregory W" uniqKey="Moseley G" first="Gregory W." last="Moseley">Gregory W. Moseley</name><affiliation><nlm:aff id="a1"><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne</institution>, Melbourne, Victoria, 3010,<country>Australia</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27649849</idno><idno type="pmc">5030706</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030706</idno><idno type="RBID">PMC:5030706</idno><idno type="doi">10.1038/srep33493</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000844</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000844</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Quantitative Analysis of the Microtubule Interaction of Rabies Virus P3 Protein: Roles in Immune Evasion and Pathogenesis</title><author><name sortKey="Brice, Aaron" sort="Brice, Aaron" uniqKey="Brice A" first="Aaron" last="Brice">Aaron Brice</name><affiliation><nlm:aff id="a1"><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne</institution>, Melbourne, Victoria, 3010,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Whelan, Donna R" sort="Whelan, Donna R" uniqKey="Whelan D" first="Donna R." last="Whelan">Donna R. Whelan</name><affiliation><nlm:aff id="a2"><institution>School of Chemistry, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name><affiliation><nlm:aff id="a3"><institution>Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences</institution>, Gifu University, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></nlm:aff></affiliation><affiliation><nlm:aff id="a4"><institution>The United Graduate School of Veterinary Sciences, Gifu University</institution>, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></nlm:aff></affiliation></author><author><name sortKey="Shimizu, Kenta" sort="Shimizu, Kenta" uniqKey="Shimizu K" first="Kenta" last="Shimizu">Kenta Shimizu</name><affiliation><nlm:aff id="a4"><institution>The United Graduate School of Veterinary Sciences, Gifu University</institution>, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></nlm:aff></affiliation></author><author><name sortKey="Wiltzer Bach, Linda" sort="Wiltzer Bach, Linda" uniqKey="Wiltzer Bach L" first="Linda" last="Wiltzer-Bach">Linda Wiltzer-Bach</name><affiliation><nlm:aff id="a5"><institution>Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="a6"><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Lo, Camden Y" sort="Lo, Camden Y" uniqKey="Lo C" first="Camden Y." last="Lo">Camden Y. Lo</name><affiliation><nlm:aff id="a7"><institution>Monash Micro Imaging</institution>, 27-31 Wright Street, Clayton, Victoria, 3168,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Blondel, Danielle" sort="Blondel, Danielle" uniqKey="Blondel D" first="Danielle" last="Blondel">Danielle Blondel</name><affiliation><nlm:aff id="a8"><institution>Unité de Virologie Moleculaire et Structurale</institution>, CNRS, UPR 3296, 91198 Gif sur Yvette Cedex,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Jans, David A" sort="Jans, David A" uniqKey="Jans D" first="David A." last="Jans">David A. Jans</name><affiliation><nlm:aff id="a5"><institution>Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Bell, Toby D M" sort="Bell, Toby D M" uniqKey="Bell T" first="Toby D. M." last="Bell">Toby D. M. Bell</name><affiliation><nlm:aff id="a2"><institution>School of Chemistry, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Moseley, Gregory W" sort="Moseley, Gregory W" uniqKey="Moseley G" first="Gregory W." last="Moseley">Gregory W. Moseley</name><affiliation><nlm:aff id="a1"><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne</institution>, Melbourne, Victoria, 3010,<country>Australia</country></nlm:aff></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Although microtubules (MTs) are known to have important roles in intracellular transport of many viruses, a number of reports suggest that specific viral MT-associated proteins (MAPs) target MTs to subvert distinct MT-dependent cellular processes. The precise functional importance of these interactions and their roles in pathogenesis, however, remain largely unresolved. To assess the association with disease of the rabies virus (RABV) MAP, P3, we quantitatively compared the phenotypes of P3 from a pathogenic RABV strain, Nishigahara (Ni) and a non-pathogenic Ni-derivative strain, Ni-CE. Using confocal/live-cell imaging and <italic>d</italic>STORM super-resolution microscopy to quantify protein interactions with the MT network and with individual MT filaments, we found that the interaction by Ni-CE-P3 is significantly impaired compared with Ni-P3. This correlated with an impaired capacity to effect association of the transcription factor STAT1 with MTs and to antagonize interferon (IFN)/STAT1-dependent antiviral signaling. Importantly, we identified a single mutation in Ni-CE-P3 that is sufficient to inhibit MT-association and IFN-antagonist function of Ni-P3, and showed that this mutation alone attenuates the pathogenicity of RABV. These data provide evidence that the viral protein-MT interface has important roles in pathogenesis, suggesting that this interface could provide targets for vaccine/antiviral drug development.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Allan, V J" uniqKey="Allan V">V. J. Allan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Forges, H" uniqKey="De Forges H">H. de Forges</name></author><author><name sortKey="Bouissou, A" uniqKey="Bouissou A">A. Bouissou</name></author><author><name sortKey="Perez, F" uniqKey="Perez F">F. Perez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hirokawa, N" uniqKey="Hirokawa N">N. Hirokawa</name></author><author><name sortKey="Niwa, S" uniqKey="Niwa S">S. Niwa</name></author><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27649849</article-id><article-id pub-id-type="pmc">5030706</article-id><article-id pub-id-type="pii">srep33493</article-id><article-id pub-id-type="doi">10.1038/srep33493</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Quantitative Analysis of the Microtubule Interaction of Rabies Virus P3 Protein: Roles in Immune Evasion and Pathogenesis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Brice</surname><given-names>Aaron</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Whelan</surname><given-names>Donna R.</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Ito</surname><given-names>Naoto</given-names></name><xref ref-type="aff" rid="a3">3</xref><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>Shimizu</surname><given-names>Kenta</given-names></name><xref ref-type="aff" rid="a4">4</xref></contrib><contrib contrib-type="author"><name><surname>Wiltzer-Bach</surname><given-names>Linda</given-names></name><xref ref-type="aff" rid="a5">5</xref><xref ref-type="aff" rid="a6">6</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Lo</surname><given-names>Camden Y.</given-names></name><xref ref-type="aff" rid="a7">7</xref></contrib><contrib contrib-type="author"><name><surname>Blondel</surname><given-names>Danielle</given-names></name><xref ref-type="aff" rid="a8">8</xref></contrib><contrib contrib-type="author"><name><surname>Jans</surname><given-names>David A.</given-names></name><xref ref-type="aff" rid="a5">5</xref></contrib><contrib contrib-type="author"><name><surname>Bell</surname><given-names>Toby D. M.</given-names></name><xref ref-type="corresp" rid="c1">a</xref><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Moseley</surname><given-names>Gregory W.</given-names></name><xref ref-type="corresp" rid="c2">b</xref><xref ref-type="aff" rid="a1">1</xref></contrib><aff id="a1"><label>1</label><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne</institution>, Melbourne, Victoria, 3010,<country>Australia</country></aff><aff id="a2"><label>2</label><institution>School of Chemistry, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></aff><aff id="a3"><label>3</label><institution>Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences</institution>, Gifu University, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></aff><aff id="a4"><label>4</label><institution>The United Graduate School of Veterinary Sciences, Gifu University</institution>, 1-1 Yanagido, Gifu 501-1193,<country>Japan</country></aff><aff id="a5"><label>5</label><institution>Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></aff><aff id="a6"><label>6</label><institution>Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Monash University</institution>, Clayton, Victoria 3800,<country>Australia</country></aff><aff id="a7"><label>7</label><institution>Monash Micro Imaging</institution>, 27-31 Wright Street, Clayton, Victoria, 3168,<country>Australia</country></aff><aff id="a8"><label>8</label><institution>Unité de Virologie Moleculaire et Structurale</institution>, CNRS, UPR 3296, 91198 Gif sur Yvette Cedex,<country>France</country></aff></contrib-group><author-notes><corresp id="c1"><label>a</label><email>toby.bell@monash.edu</email></corresp><corresp id="c2"><label>b</label><email>gregory.moseley@unimelb.edu.au</email></corresp><fn id="n1"><label>*</label><p>Present address: Institute of Medical Virology, University Clinic Tuebingen, Elfriede-Aulhorn-Straße 6, 72076 Tübingen, Germany.</p></fn></author-notes><pub-date pub-type="epub"><day>21</day><month>09</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>6</volume><elocation-id>33493</elocation-id><history><date date-type="received"><day>13</day><month>05</month><year>2016</year></date><date date-type="accepted"><day>25</day><month>08</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2016, The Author(s)</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><pmc-comment>author-paid</pmc-comment>
<license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract><p>Although microtubules (MTs) are known to have important roles in intracellular transport of many viruses, a number of reports suggest that specific viral MT-associated proteins (MAPs) target MTs to subvert distinct MT-dependent cellular processes. The precise functional importance of these interactions and their roles in pathogenesis, however, remain largely unresolved. To assess the association with disease of the rabies virus (RABV) MAP, P3, we quantitatively compared the phenotypes of P3 from a pathogenic RABV strain, Nishigahara (Ni) and a non-pathogenic Ni-derivative strain, Ni-CE. Using confocal/live-cell imaging and <italic>d</italic>STORM super-resolution microscopy to quantify protein interactions with the MT network and with individual MT filaments, we found that the interaction by Ni-CE-P3 is significantly impaired compared with Ni-P3. This correlated with an impaired capacity to effect association of the transcription factor STAT1 with MTs and to antagonize interferon (IFN)/STAT1-dependent antiviral signaling. Importantly, we identified a single mutation in Ni-CE-P3 that is sufficient to inhibit MT-association and IFN-antagonist function of Ni-P3, and showed that this mutation alone attenuates the pathogenicity of RABV. These data provide evidence that the viral protein-MT interface has important roles in pathogenesis, suggesting that this interface could provide targets for vaccine/antiviral drug development.</p></abstract></article-meta></front><body><p>The microtubule (MT) cytoskeleton is an extensive cytoplasmic network of filaments composed of α-/β-tubulin subunits, which has diverse functions in cellular processes including signal transduction, vesicular and organelle transport, and nucleocytoplasmic protein trafficking, many of which are regulated by a class of cellular proteins called MT-associated proteins (MAPs)<xref ref-type="bibr" rid="b1">1</xref><xref ref-type="bibr" rid="b2">2</xref><xref ref-type="bibr" rid="b3">3</xref><xref ref-type="bibr" rid="b4">4</xref>. Viruses commonly exploit MT-dependent cytoplasmic trafficking to deliver virions/genomes to sites of replication and egress<xref ref-type="bibr" rid="b5">5</xref><xref ref-type="bibr" rid="b6">6</xref><xref ref-type="bibr" rid="b7">7</xref><xref ref-type="bibr" rid="b8">8</xref>, but several reports suggest that viral interactions with MTs might also enable subversion of the biology of infected cells <italic>via</italic> discrete interactions formed by specific “viral MAPs”<xref ref-type="bibr" rid="b9">9</xref>. Among these is P3 protein of the lethal lyssavirus rabies virus (RABV), an isoform of the phosphoprotein (P protein) that is expressed in infected cells<xref ref-type="bibr" rid="b10">10</xref>.</p><p>P protein isoforms have been shown to have antagonistic function toward antiviral innate immune signaling by type-I interferon (IFN) cytokines, by inhibiting both IFN induction and IFN-dependent signaling<xref ref-type="bibr" rid="b11">11</xref><xref ref-type="bibr" rid="b12">12</xref><xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b14">14</xref><xref ref-type="bibr" rid="b15">15</xref><xref ref-type="bibr" rid="b16">16</xref>. The latter involves interaction of P protein with the transcription factors signal transducers and activators of transcription (STATs), that mediate intracellular signaling by IFNs, to inhibit their nuclear translocation, DNA interaction and consequent activation of IFN-stimulated gene expression<xref ref-type="bibr" rid="b11">11</xref><xref ref-type="bibr" rid="b12">12</xref><xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b14">14</xref><xref ref-type="bibr" rid="b15">15</xref><xref ref-type="bibr" rid="b16">16</xref><xref ref-type="bibr" rid="b17">17</xref><xref ref-type="bibr" rid="b18">18</xref>. Notably, P3 has been shown to interact with MTs to antagonize IFN signaling through a mechanism dependent on the integrity of the MT cytoskeleton, a property also observed in cells infected by RABV<xref ref-type="bibr" rid="b19">19</xref>. This appears to involve association of STAT1 with MTs <italic>via</italic> P3, which results in inhibition of STAT1 nuclear translocation<xref ref-type="bibr" rid="b19">19</xref>. However, in common with many other viral MAPs, the relationship of the P3-MT interaction with viral pathogenesis has not been directly examined, due primarily to the lack of specific inhibitory mutations with which the functional significance of the interaction can be interrogated using P3 protein or infectious virus.</p><p>To address this issue, we have analyzed the MT-association and IFN-antagonist function of P3 proteins derived from two strains of RABV, the pathogenic, IFN-resistant strain Nishigahara (Ni), and the non-pathogenic, IFN-sensitive derivative strain of Ni, Ni-CE<xref ref-type="bibr" rid="b20">20</xref><xref ref-type="bibr" rid="b21">21</xref>. Recent analysis of these strains identified the P protein-encoding <italic>P</italic> gene as a critical determinant of viral susceptibility to IFN and of pathogenicity in mice, by showing that replacement of the Ni-CE <italic>P</italic> gene with that of Ni (generating the recombinant CE-NiP virus) resulted in increased IFN resistance due to increased IFN-antagonistic function, and greater virulence following intracerebral inoculation<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b20">20</xref><xref ref-type="bibr" rid="b21">21</xref><xref ref-type="bibr" rid="b22">22</xref>. Thus, this system provides a means to analyze potential mechanisms of IFN antagonism and their roles in disease. To assess P3-MT interactions, we used confocal laser scanning microscopy (CLSM) for a newly developed quantitative live-cell assay of protein-MT association, and super-resolution direct stochastic optical reconstruction microscopy (<italic>d</italic>STORM). The latter is a single-molecule localization technique that detects photon emissions from single, spatially and temporally distinct, fluorescent molecules to precisely localize individual fluorophores; this can achieve c. 20 nm spatial resolution to enable analysis of structural changes to single MT filaments (c. 25 nm diameter). Using these approaches together with quantitative colocalization analysis, yeast-2-hybrid assays, IFN-dependent reporter gene assays, and viral reverse genetics/animal infection, we show that MT-interaction and IFN/STAT1 antagonism differs significantly between P3 protein from the pathogenic and non-pathogenic viruses. We further identify a single residue mutation (N<sub>226</sub>-H) that significantly inhibits both processes, enabling the first direct analysis of the role of this interaction in viral pathogenicity in mice.</p><sec disp-level="1"><title>Results and Discussion</title><sec disp-level="2"><title>MT-interaction differs significantly between P3 from pathogenic and non-pathogenic RABV</title><p>To assess the relationship of P3-MT-association with pathogenicity we expressed P3 from the Ni and Ni-CE RABV strains fused to green fluorescent protein (GFP; <xref ref-type="fig" rid="f1">Fig. 1</xref>) in a panel of mammalian cell lines from neuronal and extraneural tissues of several RABV susceptible/host species<xref ref-type="bibr" rid="b23">23</xref><xref ref-type="bibr" rid="b24">24</xref><xref ref-type="bibr" rid="b25">25</xref><xref ref-type="bibr" rid="b26">26</xref>, including COS-7 (African green monkey kidney), NSC-34 (mouse motor neuron-like), SK-N-SH (human neuronal), HeLa (human epithelial) and NA (mouse neuroblastoma). Expression of the proteins was confirmed by Western analysis lysates of COS-7 cells (see <xref ref-type="supplementary-material" rid="S1">supplemental Fig. S1</xref>).</p><p>We previously found that MT interaction by GFP-fused P3 of the RABV CVSII strain can be detected in living cells by CLSM as association with a cytoplasmic filamentous network<xref ref-type="bibr" rid="b19">19</xref>. Consistent with this, extensive interaction of GFP-Ni-P3 with cytoplasmic filaments was observed in all cell types tested (COS-7, NSC-34, shown in <xref ref-type="fig" rid="f2">Fig. 2a</xref>, and SK-N-SH, HeLa, NA, not shown). Importantly, GFP-Ni-CE-P3 filament association was clearly impaired in each of these cell types and comparable differences were observed between non-fused Ni-P3 and Ni-CE-P3 in fixed immunostained COS-7 cells (see <xref ref-type="supplementary-material" rid="S1">supplementary Fig. S2</xref>). The Ni-P3-associated filaments were confirmed to be MTs based on sensitivity to MT-targeting drugs (<xref ref-type="fig" rid="f2">Fig. 2b</xref>), and colocalization with mCherry-tubulin in living cells (<xref ref-type="fig" rid="f2">Fig. 2c</xref>) and endogenous tubulin in immunostained cells (<xref ref-type="fig" rid="f2">Fig. 2d</xref>). Thus, MT association differs between P3 proteins from pathogenic and attenuated virus.</p><p>The Ni-CE <italic>P</italic> gene contains mutations compared with Ni <italic>P</italic> that result in 5 amino acid residue substitutions, one or more of which account for the differing function in IFN-antagonism and pathogenicity<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b20">20</xref><xref ref-type="bibr" rid="b21">21</xref>. All of the substitutions affect the P3 sequence (<xref ref-type="fig" rid="f1">Fig. 1</xref>), resulting in four proline substitutions clustered within the N-terminal region (NTR), and one histidine substitution (N<sub>226</sub>-H) in the globular C-terminal domain (CTD). Previous mapping studies indicated that the latter domain mediates association with MTs<xref ref-type="bibr" rid="b19">19</xref>. To examine the effects of specific mutations, we expressed GFP-Ni-P3 containing N<sub>226</sub>-H alone (GFP-Ni-P3-N<sub>226</sub>-H) or the NTR mutations (GFP-Ni-P3-CE<sub>NTR</sub>) for analysis as above. MT filament association of GFP-Ni-P3-N<sub>226</sub>-H, but not GFP-Ni-P3-CE<sub>NTR</sub>, was clearly impaired in COS-7, NSC-34 (<xref ref-type="fig" rid="f2">Fig. 2a</xref>), and SK-N-SH, HeLa, and NA cells (not shown).</p><p>To quantify the extent of MT network association, and effects thereon, of Ni-CE mutations we generated deconvoluted 3D images of living cells expressing GFP-fused P3 proteins (images viewed down the z-axis are shown in <xref ref-type="fig" rid="f2">Fig. 2e</xref>), and developed a methodology to detect and measure intracellular filamentous GFP using the <italic>Imaris</italic> software filament-tracing algorithm tool (see Materials and Methods); this is to our knowledge the first application of this tool to quantify protein-MT interaction in living cells. This enabled semi-automated detection of P3-associated filaments in single cells to calculate the total filament length. The corresponding <italic>mean filament length</italic> (mFL) per cell was then determined for ≥ 30 cells for each protein tested (<xref ref-type="fig" rid="f2">Fig. 2f</xref>). Results confirmed that MT-association of GFP-Ni-CE-P3 and GFP-Ni-P3-N<sub>226</sub>-H, but not GFP-Ni-P3-CE<sub>NTR</sub>, was significantly reduced compared to GFP-Ni-P3 (p < 0.0001 and p = 0.0118 for Ni-CE and Ni-P3-N<sub>226</sub>-H, respectively), with the N<sub>226</sub>-H mutation alone causing a c. 50% decrease in the mFL of Ni-P3. Thus, N<sub>226</sub>-H, but not the NTR mutations, directly impacts on P3-MT association. However, since N<sub>226</sub>-H alone does not fully recapitulate the phenotype of Ni-CE-P3 (<xref ref-type="fig" rid="f2">Fig. 2f</xref>) it appears the NTR mutations can augment the effect of N<sub>226</sub>-H, indicative of an indirect/regulatory role of the NTR.</p><p>To confirm that the reduced mFL for Ni-CE-P3 and Ni-P3-N<sub>226</sub>-H is due to decreased interaction with MTs, we performed cytoplasmic extraction of transfected cells to remove non-MT associated protein, as described<xref ref-type="bibr" rid="b27">27</xref>, before immunostaining for MTs and CLSM analysis. Quantitation of P3-MT colocalization using Pearson’s coefficient indicated that MT-association is significantly (p < 0.0001) impaired for GFP-Ni-CE-P3 and GFP-Ni-P3-N<sub>226</sub>-H compared with GFP-Ni-P3 (<xref ref-type="fig" rid="f3">Fig. 3</xref>). However, the capacity of Ni-P3-N<sub>226</sub>-H to associate with MTs remained greater than that of Ni-CE-P3, consistent with data from the live-cell filament tracing assays (<xref ref-type="fig" rid="f2">Fig. 2f</xref>).</p></sec><sec disp-level="2"><title>MT bundling by P3 is significantly impaired by N<sub>226</sub>-H mutation</title><p>Interactions of cellular and viral MAPs with MTs often induce gross structural changes in MT networks that can be detected by light microscopy/CLSM analysis and are thought to correspond to MT bundling<xref ref-type="bibr" rid="b28">28</xref><xref ref-type="bibr" rid="b29">29</xref><xref ref-type="bibr" rid="b30">30</xref><xref ref-type="bibr" rid="b31">31</xref><xref ref-type="bibr" rid="b32">32</xref><xref ref-type="bibr" rid="b33">33</xref><xref ref-type="bibr" rid="b34">34</xref><xref ref-type="bibr" rid="b35">35</xref>. Such effects could provide a means to quantify MAP-MT interactions at the level of individual MT filaments. However, light microscopy/CLSM is diffraction limited with the greatest possible spatial resolution c. 200 nm, precluding direct quantitative analysis of individual MT filaments (c. 25 nm diameter) or of the extent of filament bundling. While electron microscopy (EM) can visualize individual filaments, and has been used to confirm bundling activity of certain MAPs<xref ref-type="bibr" rid="b28">28</xref><xref ref-type="bibr" rid="b29">29</xref><xref ref-type="bibr" rid="b30">30</xref><xref ref-type="bibr" rid="b31">31</xref><xref ref-type="bibr" rid="b32">32</xref><xref ref-type="bibr" rid="b33">33</xref><xref ref-type="bibr" rid="b34">34</xref><xref ref-type="bibr" rid="b35">35</xref>, such studies are typically limited to complexes of purified protein with <italic>in vitro</italic> polymerized tubulin, or analysis of cells subjected to extensive chemical fixation procedures/sample preparation times<xref ref-type="bibr" rid="b36">36</xref><xref ref-type="bibr" rid="b37">37</xref>. Furthermore, EM precludes the use of fluorescent tags for precise localization of structures/proteins of interest in large cell populations. Thus, to directly detect and quantify cellular MT filament bundling induced by P3, we utilized <italic>d</italic>STORM super-resolution imaging, which overcomes the diffraction limit by detecting unperturbed emission point spread functions from single molecules, enabling highly precise spatial localization of the emitting fluorophores. Analysis used cell fixation and immunostaining protocols known to preserve MT integrity, which we have been used previously to visualize individual MTs<xref ref-type="bibr" rid="b38">38</xref><xref ref-type="bibr" rid="b39">39</xref><xref ref-type="bibr" rid="b40">40</xref>, with <italic>d</italic>STORM measurements made using a custom-built super-resolution widefield microscope that can achieve single molecule localization precisions better than 10 nm, and spatial resolution down to 20 nm<xref ref-type="bibr" rid="b41">41</xref>. Detailed images of MT architecture were acquired with sufficient resolution to differentiate closely associated MTs (presumed to be bundles, see below) from individual MTs which are tens of nanometers apart but occupy the same diffraction limited area and so cannot be resolved using conventional light microscopy (see <xref ref-type="supplementary-material" rid="S1">supplementary Fig. S3</xref>).</p><p>To quantitatively analyze the dimensions of the filaments and occurrence of bundling in <italic>d</italic>STORM images, we calculated the relative MT <italic>feature diameter</italic> (MT<italic>fd</italic>) using the width at half-height of a Gaussian function fit to the average intensity profile of a cross-section of a continuous MT feature (<xref ref-type="fig" rid="f4">Fig. 4a</xref>). The width of single MT filaments imaged by this method will be greater than the intrinsic diameter of a MT filament (c. 25 nm) since it is the locations of antibody-conjugated Alexa Fluor-647 molecules that are detected. Thus, the primary immunolabel against tubulin and the Alex Fluor 647-conjugated secondary immunolabel (which label both sides of the filament) add some 30–40 nm to the apparent width of a single MT. Taking this and the localization precision of the fluorophores themselves (typically 9–15 nm in these measurements) into account, we predicted a range of 40–100 nm for single MTs. In agreement with this, benchmarking using mock-transfected cells indicated that 72% of MT<italic>fd</italic>s were in this range (see <xref ref-type="supplementary-material" rid="S1">supplementary Fig. S3</xref>). The thicker filaments detected in mock transfected cells (>100 nm) are likely the result of native bundling of MTs as well as detection of overlapping MTs that are separated in axial space but appear to associate in the 2D <italic>d</italic>STORM image. Nevertheless, the majority of features are within the range expected for a population of largely individual, non-bundled MTs.</p><p>Analysis of GFP-Ni-P3-expressing cells (<xref ref-type="fig" rid="f4">Fig. 4a</xref>) indicated a substantial change in MT architecture, with 63.4% of MT<italic>fd</italic>s exceeding 40–100 nm, and 18.1% exceeding 200 nm (<xref ref-type="fig" rid="f4">Fig. 4b</xref>), indicative of extensive bundling. The notion that these large MT features correspond to multiplex bundles of single MT filaments is supported by the observation that the thicker filaments often appeared to split into thinner filaments, with the continuous length of the “bundled” region indicative of specific association of the constituent MTs (see <xref ref-type="supplementary-material" rid="S1">supplementary Fig. S4</xref>).</p><p>The architecture of MT networks in cells expressing GFP-Ni-CE-P3 and GFP-Ni-P3-N<sub>226</sub>-H (<xref ref-type="fig" rid="f4">Fig. 4a</xref>) was much more consistent with that observed in mock-transfected cells than in cells expressing GFP-Ni-P3, with 78.5% (GFP-Ni-CE-P3) and 67.3% (GFP-Ni-P3-N<sub>226</sub>-H) of MT<italic>fd</italic>s within 40–100 nm (<xref ref-type="fig" rid="f4">Fig. 4b</xref>), indicative of defective bundling by these proteins. However, Ni-P3-N<sub>226</sub>-H retained a slightly increased bundling capacity compared to Ni-CE-P3, indicated by a > 2 fold higher proportion of MT<italic>fd</italic>s in all bins of the frequency distribution exceeding 140 nm. Consistent with the observed trend, statistical analysis indicated that the median MT<italic>fd</italic> for cells expressing GFP-Ni-P3 was significantly (p < 0.0001) greater than that for GFP-Ni-P3-N<sub>226</sub>-H, which in turn was significantly (p < 0.0001) greater than that for GFP-Ni-CE-P3 (<xref ref-type="fig" rid="f4">Fig. 4c</xref>).</p><p>Thus, it appears that N<sub>226</sub>-H substantially inhibits bundling, but does not fully recapitulate the defective phenotype of Ni-CE-P3, so that the degree of bundling detected by <italic>d</italic>STORM correlates with the extent of MT network association determined in CLSM-based filament tracing and co-localization assays (<xref ref-type="fig" rid="f2">Figs 2</xref> and <xref ref-type="fig" rid="f3">3</xref>). These data indicate that <italic>d</italic>STORM provides an effective method to quantify protein-MT association at the level of individual filaments. This is to our knowledge the first application of <italic>d</italic>STORM to interrogate viral protein-MT interaction and highlights the power of <italic>d</italic>STORM to elucidate fundamental processes at the virus-host interface. Together, the data indicate that N<sub>226</sub>-H directly impacts on P3-MT-association, consistent with its localization to the CTD that contains MT-association activity (<xref ref-type="fig" rid="f1">Fig. 1</xref>)<xref ref-type="bibr" rid="b19">19</xref>.</p></sec><sec disp-level="2"><title>Interaction of P3 with RABV N-protein and homodimerisation of P protein is not impaired by mutations in Ni-CE</title><p>P protein has critical roles in viral genome replication, which requires interaction of the CTD with RABV nucleoprotein (N protein) to mediate association of P protein with N-protein-encapsidated genomic RNA (N-RNA; <xref ref-type="fig" rid="f1">Fig. 1</xref>)<xref ref-type="bibr" rid="b42">42</xref>. The CTD also mediates interaction with STAT1 (<xref ref-type="fig" rid="f1">Fig. 1</xref>), which is important to the IFN-antagonistic functions of P protein isoforms<xref ref-type="bibr" rid="b15">15</xref><xref ref-type="bibr" rid="b16">16</xref><xref ref-type="bibr" rid="b43">43</xref><xref ref-type="bibr" rid="b44">44</xref>. The reported N- and STAT1-binding sites are distinct from N<sub>226</sub><xref ref-type="bibr" rid="b44">44</xref><xref ref-type="bibr" rid="b45">45</xref><xref ref-type="bibr" rid="b46">46</xref>, and previous data indicate that they are not significantly impacted by N<sub>226</sub>-H, as Ni-CE virus is highly viable in cultured cells, and Ni-CE-P is not deficient for STAT1 interaction<xref ref-type="bibr" rid="b13">13</xref>. To confirm directly that Ni-CE-P CTD interaction with N-protein is unimpaired, we used yeast-2-hybrid analysis (<xref ref-type="fig" rid="f5">Fig. 5</xref>), which has been used extensively to identify and characterize bi-molecular interactions of P protein, including with N protein, as well as P protein homodimerisation<xref ref-type="bibr" rid="b47">47</xref><xref ref-type="bibr" rid="b48">48</xref><xref ref-type="bibr" rid="b49">49</xref>. This indicated no evident defect for Ni-CE compared with Ni P proteins. 2-hybrid analysis of homodimerization of P protein via the NTR-localized dimerization domain<xref ref-type="bibr" rid="b50">50</xref> (<xref ref-type="fig" rid="f1">Fig. 1</xref>), which has been shown to be required for P3-MT association via the CTD and for MT-dependent inhibition of STAT1<xref ref-type="bibr" rid="b19">19</xref>, also indicated no defect in Ni-CE-P (<xref ref-type="fig" rid="f5">Fig. 5</xref>). Thus, the mutations in Ni-CE do not appear to be generally detrimental to P protein structure or function, indicative of specific effects on MT interaction such that the N<sub>226</sub>-H mutation could be applied to investigate directly the functional significance of P3-MT association.</p></sec><sec disp-level="2"><title>N<sub>226</sub>-H mutation impairs IFN antagonist function of P3 and pathogenicity of RABV</title><p>To examine the effect of N<sub>226</sub>-H mutation on the capacity of P3 to cause association of STAT1 with MTs, cells expressing GFP-fused Ni-P3, Ni-CE-P3 and Ni-P3-N<sub>226</sub>-H were treated with IFNα before cytoplasmic extraction, fixation and immunostaining for STAT1 and tubulin, and imaging by CLSM. Association of GFP-Ni-P3 and STAT1 with MTs was clearly detectable (<xref ref-type="fig" rid="f6">Fig. 6a</xref>), but association of STAT1 with MTs was strongly reduced or undetectable in cells expressing GFP-Ni-P3-N<sub>226</sub>-H, and there was no evidence of STAT1-MT interaction in cells expressing GFP-Ni-CE-P3 or control cells transfected to express GFP alone. To further analyze the effect of N<sub>226</sub>-H on P3-mediated antagonism of IFNα/STAT1-dependent signaling, we used a luciferase reporter gene assay, as previously described<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b44">44</xref>. Ni-P3 strongly inhibited signaling in response to IFNα, but Ni-CE-P3 and Ni-P3-N<sub>226</sub>-H were significantly (p < 0.0001) impaired in this respect (<xref ref-type="fig" rid="f6">Fig. 6b</xref>). Notably, the IFN-antagonistic function of the different P3 proteins correlated with their capacity to associate with MTs and induce MT-association of STAT1 (<xref ref-type="fig" rid="f2">Figs 2</xref>, <xref ref-type="fig" rid="f3">3</xref>, <xref ref-type="fig" rid="f4">4</xref> and <xref ref-type="fig" rid="f6">6a</xref>), and with the pathogenicity of Ni and Ni-CE viruses.</p><p>To directly investigate the effect of N<sub>226</sub>-H on infection <italic>in vivo</italic>, we introduced this mutation alone into the Ni <italic>P</italic> gene of the pathogenic IFN-resistant CE(NiP) virus<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b21">21</xref>; the derived recombinant virus was called CE(NiP-N<sub>226</sub>-H) (<xref ref-type="fig" rid="f7">Fig. 7a</xref>). ddY mice (five mice per virus) were inoculated intracerebrally with 100 focus forming units (FFU) of Ni-CE, CE(NiP) or CE(NiP-N<sub>226</sub>-H) virus, and monitored over 21 days post-infection (dpi), as previously<xref ref-type="bibr" rid="b13">13</xref>. Consistent with previous observations, mock infection was asymptomatic (data not shown), with infection by Ni-CE virus causing only mild and temporary weight changes, while CE(NiP) caused death or sacrifice at the defined end-point in 1 out of 5 mice by 11 dpi, and 5/5 mice by 14 dpi (<xref ref-type="fig" rid="f7">Fig. 7b</xref>). The outcome of infection with CE(NiP-N<sub>226</sub>-H) was similar to infection with Ni-CE, causing only minor temporary weight loss with no onset of major symptoms and no fatalities, indicating significant attenuation (p = 0.0027). We also performed infection using 10<sup>6</sup> FFU virus, finding that infection by Ni-CE remained non-lethal while all CE(NiP) infected mice succumbed to infection by 8 dpi. Significant (p = 0.0052) attenuation of CE(NiP-N<sub>226</sub>-H) was observed an the increased FFU, with only 3/5 mice succumbing by 14 dpi while the remaining mice showed increasing weight from 11 dpi.</p><p>Taken together, our data indicate that N<sub>226</sub>-H mutation impairs the capacity of P3 to interact with MTs and effect antagonism of antiviral signaling, and strongly reduces the ability of RABV to cause lethal infection in mice. These findings are consistent with key roles for MT-dependent IFN-antagonism through the P3-MT interface in the pathogenicity of RABV, supporting the hypothesis that viral protein-MT interactions have significant roles in subversion of host biology distinct from well-established functions of MTs in virus trafficking<xref ref-type="bibr" rid="b9">9</xref>. Importantly, the observation that modification of these interactions can affect disease outcomes <italic>in vivo</italic> identifies new potential targets at the virus-MT interface that could contribute to the development of attenuated vaccines and/or antiviral drugs to combat infection by lyssaviruses, which cause rabies disease with an almost 100% case-fatality rate and no effective therapeutic options<xref ref-type="bibr" rid="b51">51</xref>.</p></sec></sec><sec disp-level="1"><title>Materials and Methods</title><sec disp-level="2"><title>Ethics statement</title><p>Animal experiments were conducted in strict accordance with the <italic>Regulations for Animal Experiments</italic> in Gifu University and the <italic>Standards Relating to the Care and Management of Experimental Animals</italic> (Notice No. 6 of the Prime Minister’s office, March 27, 1980), based on the <italic>Law for the Humane Treatment and Management of Animals</italic> (Ministry of the Environment, Japan). The protocols were approved by the <italic>Committee for Animal Research and Welfare</italic> of Gifu University (Approval Number 08119).</p></sec><sec disp-level="2"><title>Constructs</title><p>Constructs were generated by PCR amplification of inserts from Ni and Ni-CE P gene cDNA<xref ref-type="bibr" rid="b13">13</xref> for cloning into the mammalian expression vector pEGFP-C1 (to express GFP-fused protein) or pΔEGFPC1 (to express untagged protein), as previously<xref ref-type="bibr" rid="b52">52</xref><xref ref-type="bibr" rid="b53">53</xref>. Ni-P3-N<sub>226</sub>-H and Ni-P3-CE<sub>NTR</sub> cDNA was generated by PCR overlap mutagenesis. For yeast-2-hybrid analysis, cDNA encoding RABV P or N protein sequences were cloned into pLex or pGAD (Clontech) plasmids. The mCherry-tubulin plasmid was a kind gift of R. Tsien (University of California)<xref ref-type="bibr" rid="b54">54</xref>. Luciferase assays used the plasmids pRL-tk (encoding <italic>Renilla</italic> luciferase under the control of a constitutively active promoter) and pISRE-luc (encoding <italic>Firefly</italic> luciferase under the control of an IFNα inducible promoter)<xref ref-type="bibr" rid="b43">43</xref><xref ref-type="bibr" rid="b44">44</xref><xref ref-type="bibr" rid="b55">55</xref>. All constructs were verified by Sanger sequencing.</p></sec><sec disp-level="2"><title>Cell culture, transfection and drug treatments</title><p>COS-7 and NSC-34 cells were cultured in DMEM supplemented with 10% FCS (37 °C, 5% CO<sub>2</sub>). Cells were grown to 80% confluency in 6-well culture dishes (for luciferase assays), on coverslips in 6-well culture dishes (for live-cell CLSM) or 12-well culture dishes (for fixed CLSM), or in 8-well Lab-Tek chambered coverglass (Nunc; for <italic>d</italic>STORM), before transfection using Lipofectamine 2000 or FugeneHD according to the manufacturer’s instructions. To disrupt or stabilize MTs, cells were treated with nocodazole (Sigma; 4 h, 5 μg/ml) or Taxol (Sigma; 4 h, 1 μg/ml), respectively<xref ref-type="bibr" rid="b19">19</xref>.</p></sec><sec disp-level="2"><title>Confocal laser scanning microscopy</title><p>For live cell analysis, cells were imaged in phenol free DMEM in a 37 °C heated chamber. For immunostaining of untagged P protein, cells were fixed with 100% methanol (10 mins, −20 °C) and immunostained using anti-P protein antibody<xref ref-type="bibr" rid="b56">56</xref> and Alexa Fluor-568-conjugated secondary antibody (Molecular Probes) before mounting in Gold antifade mountant (Life Technologies). For immunostaining of MTs and STAT1, cells were treated with or without 1000 U/ml recombinant human IFNα (PBL Interferon Source) for 30 minutes before fixation with 3% paraformaldehyde/0.1% glutaraldehyde (10 minutes, 37 °C) and staining with anti-STAT1 (Santa Cruz) and/or anti-β-tubulin (Sigma), followed by Alexa Fluor-647 or Alexa Fluor-568-conjugated secondary antibody (Molecular Probes). To extract soluble (non-MT associated) protein, we used a modified version of the protocol of Zhai <italic>et al</italic>. 1996<xref ref-type="bibr" rid="b27">27</xref> whereby cells were incubated in MT-stabilizing buffer (60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl<sub>2</sub>, pH 6.9) containing 0.5% Triton-X100 (3 minutes, 37 °C) prior to fixation as above.</p><p>CLSM analysis used a Nikon Eclipse C1, Leica SP5 or Olympus fluorview FV1000 inverted confocal laser scanning microscope with 60x, 63x or 100x oil-immersion objective. Image acquisition used Nikon NIS-Elements (Eclipse C1), Leica LAS AF (SP5) or Olympus fluoview FV10-ASW (FV1000) software. Digitized confocal files (single slices) were processed using ImageJ 1.62 software (NIH). For colocalization analysis, Pearson’s coefficient was calculated using Velocity software (PerkinElmer).</p></sec><sec disp-level="2"><title>3D live-cell imaging and calculation of mean filament length (mFL)</title><p>To generate deconvoluted 3D images of live cells, the point spread function of a Nikon Eclipse C1 inverted confocal laser scanning microscope with 60 × 1.4 NA oil immersion lens was calculated using 0.2 μm green fluorescent beads (Invitrogen). Images of beads were sampled along the z-axis at 0.25 μm intervals, and a 3D image of a single bead reconstructed from the z-stack was subjected to deconvolution using AutoQuant X software (MediaCybernetics; 20 iterations, high noise filtering). Live COS-7 cells expressing GFP-Ni-P3 or derivatives thereof were then imaged using identical conditions to generate 3D imaged from z-stacks, and these were deconvoluted using the determined point spread function.</p><p>For filament tracing, 3D images were analyzed using the Imaris (Bitplane) software. A typical image of a GFP-Ni-P3-expressing cell was initially analyzed to determine standard settings to detect filaments based on the difference in contrast between filamentous fluorescence and diffuse cytoplasmic fluorescence using the <italic>filament tracing tool algorithm</italic>. The settings were subsequently used for the analysis of all cells (n ≥ 30 cells for each construct tested). The total length of GFP-associated filaments for individual cells was calculated using the Imaris <italic>filament length (sum)</italic> value, and the mean value for n ≥ 30 cells (mFL) was calculated for each protein. Cells without traceable filaments were given a mFL of zero.</p></sec><sec disp-level="2"><title>Dual luciferase reporter assays</title><p>COS-7 cells were transfected with pRL-TK and pISRE-luc plasmids, and with plasmid encoding GFP-fused Ni-P3, Ni-CE-P3, Ni-P3-N<sub>226</sub>H or GFP alone, as previously<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b55">55</xref>. Cells were treated 21 h post-transfection with or without 500 U/ml recombinant human IFNα (PBL Interferon Source) for 6 h, before measurement of luciferase activity according to the manufacturer’s protocol (Dual-Luciferase Reporter Assay System; Promega). Normalized luciferase activity was calculated by dividing Firefly luciferase values by Renilla luciferase values, before determination of the fold change relative to values obtained for IFNα-treated cells expressing wild-type Ni-P3 protein.</p></sec><sec disp-level="2"><title><italic>d</italic>STORM microscopy</title><p>Cells were fixed 18 h post-transfection using 3% paraformaldehyde/0.1% glutaraldehyde (37 °C, 10 min), before permeabilization using 1% Triton-X100 (5 min) and blocking with 1% bovine serum albumin in phosphate buffered saline (PBS; 1 hr). Cells were then immunostained using anti-β-tubulin antibody (Sigma) and Alexa Fluor-647-conjugated secondary antibody (Molecular Probes), followed by a second fixation with 3% paraformaldehyde/0.1% glutaraldehyde (10 min). Fixed cells were imaged in a buffer containing 10% glucose, 120 mM mercaptoethylamine, 200 μg/mL glucose oxidase, and 20 μg/mL catalase in isotonic PBS (pH 8).</p><p>Cells were viewed using a custom-built widefield fluorescence microscope<xref ref-type="bibr" rid="b41">41</xref> (Olympus IX71, 100 × 1.49 NA TIRF objective) with epifluorescence used to select GFP-positive cells (488 nm excitation). Excitation with a high power red laser (Oxxius Laser Boxx, 638 nm, ~3–5 kW cm<sup>2</sup>) was used to induce photoswitching of the Alexa Fluor-647 molecules. Once satisfactory photoswitching had been achieved, cells were imaged at 100 Hz over several minutes using an electron multiplying CCD camera (Andor Ixon Ultra) to collect a TIFF stack of 5,000–20,000 consecutive images containing single molecule emissions. Raw TIFF stacks were imported into rapi<italic>d</italic>STORM<xref ref-type="bibr" rid="b57">57</xref> and analyzed using an input pixel size of 10 nm and a point spread function full width half height diameter of 370 nm, as determined using the ‘estimate PSF form’. Localizations of emissions from single molecules were only calculated when more than 500 photons were detected from a single location within a frame. These point spread functions were fit using a 2D Gaussian function and the resulting list of co-ordinates rendered into images using 10 nm pixels. Localization precisions were estimated using the Thompson equation<xref ref-type="bibr" rid="b58">58</xref> while spatial resolution is quoted as ‘as good as’ figures based on cross-sections of features within images discernible by separation at half height.</p><p>To quantitate the occurrence of MT-bundling, a 10 × 10 grid was placed over a 25 μm<sup>2</sup> region for each cell image and the average intensity profile was taken longitudinally across all filaments within each grid box. Gaussian functions were then fit to each average intensity profile and the MT <italic>feature diameter</italic> (MT<italic>fd</italic>) derived from the full width half-height.</p></sec><sec disp-level="2"><title>Yeast two-hybrid assays</title><p>Yeast L40 cells were co-transformed with pLex and pGAD constructs containing DNA encoding the indicated Lex- or GAD-fused proteins for an X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactosidase) overlay assay. Briefly, an X-Gal mixture containing 0.5% agar, 0.1% SDS, 6% dimethylformamide and 0.04% X-Gal was overlaid on fresh transformants grown on medium lacking tryptophan and leucine, and the appearance of growth streaks assessed up to 18 h at 30 °C; blue coloration (due to β-galactosidase activity in the X-Gal-containing medium) is indicative of interaction of Lex- and GAD-fused proteins.</p></sec><sec disp-level="2"><title>Generation of recombinant virus</title><p>Ni-CE and CE(NiP) viruses have been described previously<xref ref-type="bibr" rid="b20">20</xref>. Full-genome plasmid for CE(NiP-N<sub>226</sub>-H) was constructed using Ni-CE strain plasmid by conventional molecular cloning techniques (detailed information is available on request) and the mutant virus was recovered as described previously<xref ref-type="bibr" rid="b59">59</xref>. P gene sequences were confirmed by sequencing of RT-PCR fragments from the recombinant viruses, and viral stocks were propagated in mouse neuroblastoma NA cells.</p></sec><sec disp-level="2"><title>Pathogenicity studies</title><p>Five 4-week-old female ddY mice (Japan SLC Inc., Hamamatsu, Japan) were intracerebrally inoculated with 0.03 ml dilutent (E-MEM with 5% FCS) containing 100 FFU virus, 10<sup>6</sup> FFU of virus or no virus (mock infection). The weight of individual mice was measured daily over 14 or 21 dpi and body weight change calculated relative to the weight at 0 dpi. Mice failing to show righting reflexes in body tilt experiments (end-point) were sacrificed.</p></sec><sec disp-level="2"><title>Statistical analysis</title><p>Prism version 6 software (Graphpad) was used for statistical analysis to calculate p-values using Student’s t-test (unpaired, two-tailed). If variances were determined to be significantly different (F-test) a Welch’s correction was applied. If datasets failed the D’Agostino-Pearson omnibus normality test, the alternative Mann-Whitney test was used. To calculate p-values for survival curves the log-rank (Mantel-Cox) test was used.</p></sec></sec><sec disp-level="1"><title>Additional Information</title><p><bold>How to cite this article</bold>: Brice, A. <italic>et al</italic>. Quantitative Analysis of the Microtubule Interaction of Rabies Virus P3 Protein: Roles in Immune Evasion and Pathogenesis. <italic>Sci. Rep.</italic><bold>6</bold>, 33493; doi: 10.1038/srep33493 (2016).</p></sec><sec sec-type="supplementary-material" id="S1"><title>Supplementary Material</title><supplementary-material id="d33e26" content-type="local-data"><caption><title>Supplementary Information</title></caption><media xlink:href="srep33493-s1.pdf"></media></supplementary-material></sec></body><back><ack><p>We acknowledge Cassandra David for assistance with tissue culture, the facilities and technical assistance of Monash Micro Imaging (Monash University) and the Biological Optical Microscopy Platform (University of Melbourne) for confocal imaging, Baptiste Fouquet and Alexandre Berard for their help with two-hybrid experiments, and Steven Rawlinson and Kim Gia Lieu for critical reading of the manuscript. The NSC-34 cells were a kind gift from Danny Hatters (University of Melbourne). This work was supported by: National Health and Medical Research Council Australia project grant #1003244 and #1079211 to GWM and #1052171 to TDMB; Australian Research Council Discovery project grant #DP110101749 to GWM and DAJ, DP150102569 to GWM and DP130101861 to TDMB; National Health and Medical Research Council Australia Senior Principal Research Fellowship #1002486 to DAJ; Grimwade Fellowship (Miegunyah Trust) to GWM; Japanese Society for the Promotion of Science, Invitation Fellowship Program for Research in Japan (L14563 and S11102) to GWM. We also acknowledge the Monash Faculty of Science Dean’s strategic funding initiative to TDMB and Microbiology@Monash for a collaborative project grant to AB and DRW.</p></ack><ref-list><ref id="b1"><mixed-citation publication-type="journal"><name><surname>Allan</surname><given-names>V. 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A.B. generated the P3 constructs and performed the confocal imaging analyses, luciferase assays, analysis of <italic>d</italic>STORM data to determine MT<italic>fd</italic>s and, with C.Y.L. developed the protocol for filament tracing analysis. L.W.-B. assisted with the cloning, confocal imaging and luciferase experiments. D.R.W. performed the <italic>d</italic>STORM imaging and with T.D.M.B. developed the protocol for MT<italic>fd</italic> analysis. N.I. provided the cDNA for cloning of P3 and with K.S. performed the viral reverse genetics and infection studies. D.B. generated the pLEX and pGAD constructs and performed the yeast-2-hybrid experiments. G.W.M., A.B., D.R.W. and T.D.M.B. contributed to the writing of the manuscript and, with N.I. and K.S., generated the figures.</p></fn></fn-group></back><floats-group><fig id="f1"><label>Figure 1</label><caption><title>Schematic representation of P protein and the P3 proteins used in this study.</title><p>P3 is an N-terminally truncated isoform of P protein comprising residues 53–297, which is generated in infected cells by ribosomal leaky scanning that results in translation from an internal AUG codon corresponding to M<sub>53</sub> (arrow) of the full length P protein (called P1)<xref ref-type="bibr" rid="b10">10</xref>. The CTD (containing the MT, N-RNA and STAT1 binding regions) and NTR (containing the dimerization domain) are indicated; residue positions are indicated beneath the P1 protein. Residues at positions 56, 58, 66, 81 and 226 differ between P3 from the pathogenic (Ni) and attenuated (Ni-CE) strains of RABV (substitutions in Ni-CE-P3 are in red).</p></caption><graphic xlink:href="srep33493-f1"></graphic></fig><fig id="f2"><label>Figure 2</label><caption><title>Interaction of Ni-P3 with MTs is impaired by N<sub>226</sub>-H mutation.</title><p>(<bold>a</bold>) COS-7 cells were transfected to express the indicated proteins before analysis by live-cell CLSM; each image is representative of cells in 30 fields of view sampled over 3 separate assays (COS-7) or 9 fields of view (NSC-34). (<bold>b–d</bold>) COS-7 cells transfected to express GFP-Ni-P3 were treated with or without Taxol or nocodazole (<bold>b</bold>) co-transfected to express mCherry-tubulin (<bold>c</bold>) or fixed and immunostained for β-tubulin (<bold>d</bold>) before analysis by CLSM; colocalization in b and d is apparent as yellow coloration in merged image. (<bold>e</bold>) Live COS-7 cells expressing the indicated proteins were analyzed by CLSM to generate deconvoluted 3D images (images show reconstructed 3D images viewed down the z-axis). (<bold>f</bold>) Images such as those shown in (<bold>e</bold>) were analyzed to derive mean filament length values (mFL ± SEM; n ≥ 30 cells from 3 identical assays). p values were determined using the Mann Whitney test.</p></caption><graphic xlink:href="srep33493-f2"></graphic></fig><fig id="f3"><label>Figure 3</label><caption><p>N<sub>226</sub>-H mutation inhibits colocalization of P3 with MTs (<bold>a</bold>) COS-7 cells expressing the indicated proteins were extracted to remove soluble (non-MT-associated) protein before fixation and immunostaining for β-tubulin, and analysis by CLSM (colocalization is apparent as white coloration in merged image). (<bold>b</bold>) Images such as those shown in (<bold>a</bold>) were analyzed to derive Pearson’s coefficient as a measure of colocalization between GFP-P3 and β-tubulin (mean ± SEM; n ≥ 14 cells from two assays). p values were determined using Student’s t-test or the Mann-Whitney test.</p></caption><graphic xlink:href="srep33493-f3"></graphic></fig><fig id="f4"><label>Figure 4</label><caption><title>Quantitative <italic>d</italic>STORM analysis of P3-induced bundling of MTs.</title><p>(<bold>a</bold>) <italic>d</italic>STORM images of immunostained β<bold>-</bold>tubulin in COS-7 cells expressing the indicated proteins are shown in the upper panels. Boxed areas are expanded in the lower panels, and Gaussian functions (black line) fit to the average intensity profile (red line) of the indicated filaments shown below. The derived MT<italic>fd</italic> (calculated at the full width half-height of the Gaussian function) is indicated. (<bold>b</bold>) The frequency distribution of MT<italic>fd</italic>s calculated for each protein is shown (n = 1294 [GFP-Ni-P3], 1071 [GFP-Ni-CE-P3] and 1299 [GFP-Ni-P3-N<sub>226</sub>-H]; measurements are from 10 cells for each protein over two identical assays). (<bold>c</bold>) Scatter dot plots of MT<italic>fd</italic>s shown in (<bold>b</bold>). p values were determined using the Mann-Whitney test.</p></caption><graphic xlink:href="srep33493-f4"></graphic></fig><fig id="f5"><label>Figure 5</label><caption><title>Ni-CE P can interact with N-protein and contains a functional homodimerisation region.</title><p>L40 yeast cells were cotransformed to express the DNA-binding domain of LexA (Lex) or the GAL4-activation domain (GAD) fused to Ni-P1, Ni-CE-P1, Ni-P3, Ni-CE P3, the corresponding P3 NTRs (P<sub>54-172</sub>), or RABV N protein. Growth streaks are shown, where interaction of Lex- and GAD-fused proteins is indicated by dark coloration of colonies.</p></caption><graphic xlink:href="srep33493-f5"></graphic></fig><fig id="f6"><label>Figure 6</label><caption><title>N<sub>226</sub>-H mutation impairs the IFN-antagonistic function of P3.</title><p>(<bold>a</bold>) IFNα-treated COS-7 cells expressing the indicated proteins were extracted before fixation and immunostaining for STAT1 and β-tubulin, and analysis by CLSM; images are representative of 15 fields of view from two assays. (<bold>b</bold>) IFN-α-dependent signaling in COS-7 cells expressing the indicated proteins was analyzed using a dual luciferase reporter gene assay, as previously described<xref ref-type="bibr" rid="b13">13</xref><xref ref-type="bibr" rid="b19">19</xref><xref ref-type="bibr" rid="b43">43</xref>. Luciferase activity is expressed as fold change relative to that obtained for IFN-α-treated cells expressing Ni-P3 protein (mean relative light units [RLU] ± SEM; n = 12 from 4 identical assays). p values were calculated using Student’s t-test.</p></caption><graphic xlink:href="srep33493-f6"></graphic></fig><fig id="f7"><label>Figure 7</label><caption><title>N<sub>226</sub>-H mutation attenuates RABV.</title><p>(<bold>a</bold>) Schematic representation of the genomes of viruses used; genes from Ni and Ni-CE are in black and white, respectively. The <italic>P</italic> gene of Ni-CE is substituted for the <italic>P</italic> gene of Ni in CE(NiP), and for a mutated version of the Ni <italic>P</italic> gene containing N<sub>226</sub>-H in CE(NiP-N<sub>226</sub>-H). (<bold>b,c</bold>) 100 (<bold>b</bold>) or 10<sup>6</sup> (<bold>c</bold>) FFU of the indicated virus was inoculated intracerebrally into mice (five mice per condition) and body weight relative to that at 0 dpi (mean relative body weight ± SEM, for live mice) and survival was monitored over 21 or 14 dpi. p-values for survival curves were calculated using log-rank (Mantel-Cox) test. <sup>†</sup>all mice dead or sacrificed on reaching end point.</p></caption><graphic xlink:href="srep33493-f7"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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