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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multilevel Approach of a 1-Year Program of Dietary and Exercise Interventions on Bone Mineral Content and Density in Metabolic Syndrome – the RESOLVE Randomized Controlled Trial</title><author><name sortKey="Courteix, Daniel" sort="Courteix, Daniel" uniqKey="Courteix D" first="Daniel" last="Courteix">Daniel Courteix</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Valente Dos Santos, Joao" sort="Valente Dos Santos, Joao" uniqKey="Valente Dos Santos J" first="João" last="Valente-Dos-Santos">João Valente-Dos-Santos</name><affiliation><nlm:aff id="aff004"><addr-line>Lusófona University of Humanities and Technologies, Faculty of Physical Education and Sport, Lisbon, Portugal</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff005"><addr-line>University of Coimbra, Faculty of Sport Sciences and Physical Education, Research Unit for Sport and Physical Activity (CIDAF), Coimbra, Portugal</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ferry, Beatrice" sort="Ferry, Beatrice" uniqKey="Ferry B" first="Béatrice" last="Ferry">Béatrice Ferry</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lac, Gerard" sort="Lac, Gerard" uniqKey="Lac G" first="Gérard" last="Lac">Gérard Lac</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lesourd, Bruno" sort="Lesourd, Bruno" uniqKey="Lesourd B" first="Bruno" last="Lesourd">Bruno Lesourd</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff006"><addr-line>Auvergne University, Faculty of Medicine, Geriatric, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chapier, Robert" sort="Chapier, Robert" uniqKey="Chapier R" first="Robert" last="Chapier">Robert Chapier</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Naughton, Geraldine" sort="Naughton, Geraldine" uniqKey="Naughton G" first="Geraldine" last="Naughton">Geraldine Naughton</name><affiliation><nlm:aff id="aff002"><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Marceau, Geoffroy" sort="Marceau, Geoffroy" uniqKey="Marceau G" first="Geoffroy" last="Marceau">Geoffroy Marceau</name><affiliation><nlm:aff id="aff007"><addr-line>University Hospital of Clermont-Ferrand (CHU), Biochemistry, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Joao Coelho E Silva, Manuel" sort="Joao Coelho E Silva, Manuel" uniqKey="Joao Coelho E Silva M" first="Manuel" last="João Coelho-E-Silva">Manuel João Coelho-E-Silva</name><affiliation><nlm:aff id="aff005"><addr-line>University of Coimbra, Faculty of Sport Sciences and Physical Education, Research Unit for Sport and Physical Activity (CIDAF), Coimbra, Portugal</addr-line></nlm:aff></affiliation></author><author><name sortKey="Vinet, Agnes" sort="Vinet, Agnes" uniqKey="Vinet A" first="Agnès" last="Vinet">Agnès Vinet</name><affiliation><nlm:aff id="aff008"><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Walther, Guillaume" sort="Walther, Guillaume" uniqKey="Walther G" first="Guillaume" last="Walther">Guillaume Walther</name><affiliation><nlm:aff id="aff008"><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Obert, Philippe" sort="Obert, Philippe" uniqKey="Obert P" first="Philippe" last="Obert">Philippe Obert</name><affiliation><nlm:aff id="aff008"><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Dutheil, Frederic" sort="Dutheil, Frederic" uniqKey="Dutheil F" first="Frédéric" last="Dutheil">Frédéric Dutheil</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff009"><addr-line>University Hospital of Clermont-Ferrand (CHU), Preventive and Occupational Medicine, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26376093</idno><idno type="pmc">4574281</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574281</idno><idno type="RBID">PMC:4574281</idno><idno type="doi">10.1371/journal.pone.0136491</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000332</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000332</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Multilevel Approach of a 1-Year Program of Dietary and Exercise Interventions on Bone Mineral Content and Density in Metabolic Syndrome – the RESOLVE Randomized Controlled Trial</title><author><name sortKey="Courteix, Daniel" sort="Courteix, Daniel" uniqKey="Courteix D" first="Daniel" last="Courteix">Daniel Courteix</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Valente Dos Santos, Joao" sort="Valente Dos Santos, Joao" uniqKey="Valente Dos Santos J" first="João" last="Valente-Dos-Santos">João Valente-Dos-Santos</name><affiliation><nlm:aff id="aff004"><addr-line>Lusófona University of Humanities and Technologies, Faculty of Physical Education and Sport, Lisbon, Portugal</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff005"><addr-line>University of Coimbra, Faculty of Sport Sciences and Physical Education, Research Unit for Sport and Physical Activity (CIDAF), Coimbra, Portugal</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ferry, Beatrice" sort="Ferry, Beatrice" uniqKey="Ferry B" first="Béatrice" last="Ferry">Béatrice Ferry</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lac, Gerard" sort="Lac, Gerard" uniqKey="Lac G" first="Gérard" last="Lac">Gérard Lac</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lesourd, Bruno" sort="Lesourd, Bruno" uniqKey="Lesourd B" first="Bruno" last="Lesourd">Bruno Lesourd</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff006"><addr-line>Auvergne University, Faculty of Medicine, Geriatric, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chapier, Robert" sort="Chapier, Robert" uniqKey="Chapier R" first="Robert" last="Chapier">Robert Chapier</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Naughton, Geraldine" sort="Naughton, Geraldine" uniqKey="Naughton G" first="Geraldine" last="Naughton">Geraldine Naughton</name><affiliation><nlm:aff id="aff002"><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Marceau, Geoffroy" sort="Marceau, Geoffroy" uniqKey="Marceau G" first="Geoffroy" last="Marceau">Geoffroy Marceau</name><affiliation><nlm:aff id="aff007"><addr-line>University Hospital of Clermont-Ferrand (CHU), Biochemistry, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Joao Coelho E Silva, Manuel" sort="Joao Coelho E Silva, Manuel" uniqKey="Joao Coelho E Silva M" first="Manuel" last="João Coelho-E-Silva">Manuel João Coelho-E-Silva</name><affiliation><nlm:aff id="aff005"><addr-line>University of Coimbra, Faculty of Sport Sciences and Physical Education, Research Unit for Sport and Physical Activity (CIDAF), Coimbra, Portugal</addr-line></nlm:aff></affiliation></author><author><name sortKey="Vinet, Agnes" sort="Vinet, Agnes" uniqKey="Vinet A" first="Agnès" last="Vinet">Agnès Vinet</name><affiliation><nlm:aff id="aff008"><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Walther, Guillaume" sort="Walther, Guillaume" uniqKey="Walther G" first="Guillaume" last="Walther">Guillaume Walther</name><affiliation><nlm:aff id="aff008"><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Obert, Philippe" sort="Obert, Philippe" uniqKey="Obert P" first="Philippe" last="Obert">Philippe Obert</name><affiliation><nlm:aff id="aff008"><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Dutheil, Frederic" sort="Dutheil, Frederic" uniqKey="Dutheil F" first="Frédéric" last="Dutheil">Frédéric Dutheil</name><affiliation><nlm:aff id="aff001"><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff003"><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff009"><addr-line>University Hospital of Clermont-Ferrand (CHU), Preventive and Occupational Medicine, Clermont-Ferrand, France</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="sec001"><title>Background</title><p>Weight loss is a public health concern in obesity-related diseases such as metabolic syndrome (MetS). However, restrictive diets might induce bone loss. The nature of exercise and whether exercise with weight loss programs can protect against potential bone mass deficits remains unclear. Moreover, compliance is essential in intervention programs. Thus, we aimed to investigate the effects that modality and exercise compliance have on bone mineral content (BMC) and density (BMD).</p></sec><sec id="sec002"><title>Methods</title><p>We investigated 90 individuals with MetS who were recruited for the 1-year RESOLVE trial. Community-dwelling seniors with MetS were randomly assigned into three different modalities of exercise (intensive resistance, intensive endurance, moderate mixed) combined with a restrictive diet. They were compared to 44 healthy controls who did not undergo the intervention.</p></sec><sec id="sec003"><title>Results</title><p>This intensive lifestyle intervention (15–20 hours of training/week + restrictive diet) resulted in weight loss, body composition changes and health improvements. Baseline BMC and BMD for total body, lumbar spine and femoral neck did not differ between MetS groups and between MetS and controls. Despite changes over time, BMC or BMD did not differ between the three modalities of exercise and when compared with the controls. However, independent of exercise modality, compliant participants increased their BMC and BMD compared with their less compliant peers. Decreases in total body lean mass and negative energy balance significantly and independently contributed to decreases in lumbar spine BMC.</p></sec><sec id="sec004"><title>Conclusion</title><p>After the one year intervention, differences relating to exercise modalities were not evident. However, compliance with an intensive exercise program resulted in a significantly higher bone mass during energy restriction than non-compliance. Exercise is therefore beneficial to bone in the context of a weight loss program.</p></sec><sec id="sec005"><title>Trial Registration</title><p>ClinicalTrials.gov <ext-link ext-link-type="uri" xlink:href="https://clinicaltrials.gov/ct2/show/NCT00917917">NCT00917917</ext-link></p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Pedersen, Sd" uniqKey="Pedersen S">SD Pedersen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shapses, Sa" uniqKey="Shapses S">SA Shapses</name></author><author><name sortKey="Riedt, Cs" uniqKey="Riedt C">CS Riedt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hinton, Ps" uniqKey="Hinton P">PS Hinton</name></author><author><name sortKey="Lecheminant, Jd" uniqKey="Lecheminant J">JD LeCheminant</name></author><author><name sortKey="Smith, Bk" uniqKey="Smith B">BK Smith</name></author><author><name sortKey="Rector, Rs" uniqKey="Rector 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26376093</article-id><article-id pub-id-type="pmc">4574281</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0136491</article-id><article-id pub-id-type="publisher-id">PONE-D-15-01159</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Multilevel Approach of a 1-Year Program of Dietary and Exercise Interventions on Bone Mineral Content and Density in Metabolic Syndrome – the RESOLVE Randomized Controlled Trial</article-title><alt-title alt-title-type="running-head">Multilevel Approach of a 1-Year Lifestyle Intervention on Bone in MetS</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Courteix</surname><given-names>Daniel</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Valente-dos-Santos</surname><given-names>João</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ferry</surname><given-names>Béatrice</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lac</surname><given-names>Gérard</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lesourd</surname><given-names>Bruno</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref><xref ref-type="aff" rid="aff006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chapier</surname><given-names>Robert</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Naughton</surname><given-names>Geraldine</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Marceau</surname><given-names>Geoffroy</given-names></name><xref ref-type="aff" rid="aff007"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>João Coelho-e-Silva</surname><given-names>Manuel</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Vinet</surname><given-names>Agnès</given-names></name><xref ref-type="aff" rid="aff008"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Walther</surname><given-names>Guillaume</given-names></name><xref ref-type="aff" rid="aff008"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Obert</surname><given-names>Philippe</given-names></name><xref ref-type="aff" rid="aff008"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dutheil</surname><given-names>Frédéric</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref><xref ref-type="aff" rid="aff009"><sup>9</sup></xref><xref rid="cor001" ref-type="corresp">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Clermont Auvergne University, Blaise Pascal University, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological conditions (AME2P, EA3533), Clermont-Ferrand, France</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Australian Catholic University, Faculty of Health, School of Exercise Science, Melbourne, Victoria, Australia</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Research Centre in Human Nutrition (CRNH) Auvergne, Clermont-Ferrand, France</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Lusófona University of Humanities and Technologies, Faculty of Physical Education and Sport, Lisbon, Portugal</addr-line></aff><aff id="aff005"><label>5</label><addr-line>University of Coimbra, Faculty of Sport Sciences and Physical Education, Research Unit for Sport and Physical Activity (CIDAF), Coimbra, Portugal</addr-line></aff><aff id="aff006"><label>6</label><addr-line>Auvergne University, Faculty of Medicine, Geriatric, Clermont-Ferrand, France</addr-line></aff><aff id="aff007"><label>7</label><addr-line>University Hospital of Clermont-Ferrand (CHU), Biochemistry, Clermont-Ferrand, France</addr-line></aff><aff id="aff008"><label>8</label><addr-line>Laboratory of Pharm-Ecology Cardiovascular (EA4278), University of Avignon, Avignon, France</addr-line></aff><aff id="aff009"><label>9</label><addr-line>University Hospital of Clermont-Ferrand (CHU), Preventive and Occupational Medicine, Clermont-Ferrand, France</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Van Nguyen</surname><given-names>Tuan</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Garvan Institute of Medical Research, AUSTRALIA</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: DC GL BL RC PO FD. Performed the experiments: DC BL RC GM AV GW PO FD. Analyzed the data: DC BF JVdS MCS. Contributed reagents/materials/analysis tools: DC BL RC GM AV GW PO FD. Wrote the paper: DC JVdS FD. Data interpretation: DC JVdS MCS. Revised manuscript content: DC JVdS GN FD. Approved final version of manuscript: DC JVdS BF GL BL RC GN GM MCS AV GW PO FD.</p></fn><corresp id="cor001">* E-mail: <email>frederic.dutheil@acu.edu.au</email></corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>9</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>10</volume><issue>9</issue><elocation-id>e0136491</elocation-id><history><date date-type="received"><day>16</day><month>1</month><year>2015</year></date><date date-type="accepted"><day>1</day><month>8</month><year>2015</year></date></history><permissions><copyright-year>2015</copyright-year><copyright-holder>Courteix et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0136491.pdf"></self-uri><abstract><sec id="sec001"><title>Background</title><p>Weight loss is a public health concern in obesity-related diseases such as metabolic syndrome (MetS). However, restrictive diets might induce bone loss. The nature of exercise and whether exercise with weight loss programs can protect against potential bone mass deficits remains unclear. Moreover, compliance is essential in intervention programs. Thus, we aimed to investigate the effects that modality and exercise compliance have on bone mineral content (BMC) and density (BMD).</p></sec><sec id="sec002"><title>Methods</title><p>We investigated 90 individuals with MetS who were recruited for the 1-year RESOLVE trial. Community-dwelling seniors with MetS were randomly assigned into three different modalities of exercise (intensive resistance, intensive endurance, moderate mixed) combined with a restrictive diet. They were compared to 44 healthy controls who did not undergo the intervention.</p></sec><sec id="sec003"><title>Results</title><p>This intensive lifestyle intervention (15–20 hours of training/week + restrictive diet) resulted in weight loss, body composition changes and health improvements. Baseline BMC and BMD for total body, lumbar spine and femoral neck did not differ between MetS groups and between MetS and controls. Despite changes over time, BMC or BMD did not differ between the three modalities of exercise and when compared with the controls. However, independent of exercise modality, compliant participants increased their BMC and BMD compared with their less compliant peers. Decreases in total body lean mass and negative energy balance significantly and independently contributed to decreases in lumbar spine BMC.</p></sec><sec id="sec004"><title>Conclusion</title><p>After the one year intervention, differences relating to exercise modalities were not evident. However, compliance with an intensive exercise program resulted in a significantly higher bone mass during energy restriction than non-compliance. Exercise is therefore beneficial to bone in the context of a weight loss program.</p></sec><sec id="sec005"><title>Trial Registration</title><p>ClinicalTrials.gov <ext-link ext-link-type="uri" xlink:href="https://clinicaltrials.gov/ct2/show/NCT00917917">NCT00917917</ext-link></p></sec></abstract><funding-group><funding-statement>This work was funded by the Heart and Arteries Foundation (Fondation Coeur et Artères) 59200 Loos, France; <ext-link ext-link-type="uri" xlink:href="http://www.fondacoeur.com">www.fondacoeur.com</ext-link>. JVdS was partially supported by a grant from the Portuguese Foundation for Science and Technology: SFRH/BPD/100470/2014. The funding source had no role in the design, conduct, or reporting of the study.</funding-statement></funding-group><counts><fig-count count="4"></fig-count><table-count count="4"></table-count><page-count count="20"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information file.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information file.</p></notes></front><body><sec sec-type="intro" id="sec006"><title>Introduction</title><p>Most metabolic diseases are linked to excess weight or obesity [<xref rid="pone.0136491.ref001" ref-type="bibr">1</xref>]. All appropriate treatments involving weight loss therapy currently include a restrictive diet. However, restrictive diet may induce bone loss [<xref rid="pone.0136491.ref002" ref-type="bibr">2</xref>,<xref rid="pone.0136491.ref003" ref-type="bibr">3</xref>]. Bone loss accompanying body weight reduction remains debatable [<xref rid="pone.0136491.ref004" ref-type="bibr">4</xref>,<xref rid="pone.0136491.ref005" ref-type="bibr">5</xref>,<xref rid="pone.0136491.ref006" ref-type="bibr">6</xref>,<xref rid="pone.0136491.ref007" ref-type="bibr">7</xref>]. Variable results depend on age, gender and lifestyle: physical activity [<xref rid="pone.0136491.ref008" ref-type="bibr">8</xref>], and protein [<xref rid="pone.0136491.ref004" ref-type="bibr">4</xref>] or calcium intake [<xref rid="pone.0136491.ref009" ref-type="bibr">9</xref>] of individuals striving for weight loss. There is a growing consensus that the risk of bone loss [<xref rid="pone.0136491.ref010" ref-type="bibr">10</xref>] is independent of the severity of dieting, as well as the amount of body weight lost [<xref rid="pone.0136491.ref011" ref-type="bibr">11</xref>,<xref rid="pone.0136491.ref012" ref-type="bibr">12</xref>].</p><p>Discussions of the mechanisms for bone loss in weight loss programs have included the potential for a reduction of mechanical loading combined with a lack of calcium and energy intake [<xref rid="pone.0136491.ref002" ref-type="bibr">2</xref>], altered local bone cells interaction [<xref rid="pone.0136491.ref013" ref-type="bibr">13</xref>] and/or reduced expression of transcription factors influencing osteoblastogenesis [<xref rid="pone.0136491.ref014" ref-type="bibr">14</xref>]. Moreover, energy restriction can alter the plasma concentration of hormones involved in bone metabolism and increase the bone turnover in obese woman [<xref rid="pone.0136491.ref015" ref-type="bibr">15</xref>].</p><p>The specificity of metabolic syndrome (MetS) [<xref rid="pone.0136491.ref016" ref-type="bibr">16</xref>] tends to target obesity at an abdominal location [<xref rid="pone.0136491.ref016" ref-type="bibr">16</xref>]. Abdominal fat tissue produces numerous adipokines linked with inflammation [<xref rid="pone.0136491.ref017" ref-type="bibr">17</xref>]. Therefore, during lifestyle intervention, inflammatory effects from abdominal fat tissue may occur despite body weight reduction [<xref rid="pone.0136491.ref018" ref-type="bibr">18</xref>].</p><p>The benefits of weight-bearing physical activity on bone mineral content (BMC) and density (BMD) are well established [<xref rid="pone.0136491.ref019" ref-type="bibr">19</xref>], and as such, exercise training may also attenuate the decrease in BMD during a weight loss [<xref rid="pone.0136491.ref007" ref-type="bibr">7</xref>,<xref rid="pone.0136491.ref020" ref-type="bibr">20</xref>]. Nonetheless, there are conflicting reports on whether exercise accompanying a weight loss program is able to protect against a potential bone loss. Both diet-induced and exercise-induced weight loss have been linked to bone deficits [<xref rid="pone.0136491.ref021" ref-type="bibr">21</xref>]. Indeed, the bones’ response to weight loss combined with exercise could be specific to weight-bearing sites. Moreover, compliance with physical activity and dietary guidelines has been reported to be essential in the success of lifestyle interventions [<xref rid="pone.0136491.ref022" ref-type="bibr">22</xref>].</p><p>The RESOLVE trial (REverse metabolic SyndrOme by Lifestyle and Various Exercises) is an interventional study that assessed the change in elderly participants with MetS after one year [<xref rid="pone.0136491.ref023" ref-type="bibr">23</xref>]. It aimed to compare the broad health-related effects of three different modalities of exercise (intensive resistance, intensive endurance, and moderate mixed) combined with a restrictive diet. This intensive lifestyle intervention resulted in weight loss, body composition changes and improvement of participants’ health [<xref rid="pone.0136491.ref023" ref-type="bibr">23</xref>,<xref rid="pone.0136491.ref024" ref-type="bibr">24</xref>]. To understand more about associated bone changes we also assessed BMC and BMD at each step of the intervention.</p><p>Multilevel statistical models are appropriate to deal with repeated measurements. Therefore, the purpose of the present study was to determine the effects of weight loss and the associated characteristics of MetS on bone metabolism, taking into account a physical activity program implementing three different modalities of exercise. If physical activity was capable of compensating for the bone loss induced by the restrictive diet, we further investigated whether any specific modality of exercise induced a better result than the others. A second purpose was to assess the effects of compliance to diet and exercise by measuring the observance of participants during a 3-week residential program followed by 11 months of at-home intervention.</p></sec><sec sec-type="materials|methods" id="sec007"><title>Methods</title><sec id="sec008"><title>Participants</title><p>Volunteers were recruited via advertisements. They gave their written informed consent. The study was reviewed and approved by the human research ethics committee from the University Hospital of St Etienne, France. To be eligible, participants had to be: aged between 50 and 70 years, symptomatic of MetS [<xref rid="pone.0136491.ref016" ref-type="bibr">16</xref>], leading a sedentary lifestyle, stable in body weight and medical treatment over the previous 6 months, post-menopausal for women, without hepatic, renal, or psychiatric diseases, nor cardiovascular or endocrine diseases except those defining MetS, devoid of medications altering bone tissue properties, not undertaking restrictive dieting in the previous year, and able to complete a normal maximal exercise tolerance test (VO<sub>2max</sub>).</p><p>For baseline references, data were obtained from an age-matched control group of healthy participants who did not undergo any intervention. Criteria for inclusion as a healthy control included being without any of the defined criteria of MetS, chronic disease, and routine medication. They also had to report a stable lifestyle over the previous 12 months, and at the time of testing were completing less than three hours of organized physical activity per week.</p></sec><sec id="sec009"><title>Primary outcome</title><p>The primary outcome was the change in BMC and BMD for the whole body, lumbar spine and non-dominant hip. BMC and BMD were measured by dual-energy X-ray absorptiometry scans (DXA, Hologic QDR 4500 series; Waltham, USA). The in-vivo coefficient of variation (CV) was 0.5%.</p></sec><sec id="sec010"><title>Secondary outcomes</title><sec id="sec011"><title>Anthropometry</title><p>Body height was measured with a standard stadiometer (Holtain, Ltd., Crymych, UK). Fat mass and lean body mass were measured by DXA, with respective in vivo CV of 4.2 and 0.4%. Central fat, a surrogate of visceral fat, was assessed from DXA scans, as described by Kamel et al [<xref rid="pone.0136491.ref025" ref-type="bibr">25</xref>]. We determined a CV of 1.6% in the central fat measurements.</p></sec><sec id="sec012"><title>Biochemistry</title><p>Fasting blood samples were drawn between 7.00 and 7.30 a.m., aliquoted and stored at -80°C until analysis. Basic biological assays were performed in the biochemistry laboratory of the University Hospital of Clermont-Ferrand, France. Insulin, pro-inflammatory cytokines (TNF-alpha, IL-1 and IL-6), the adipokines leptin and adiponectin, and PAI-1 were assayed by ELISA using commercial kits (Millipore, Billerica, MA, USA). Sensitivity, intra- and interassay coefficients of variation were respectively 3.0 ng/ml, 2.6%, 7.2% for insulin; 0.7 pg/ml, 6.0% and 9.0%, for TNF-alpha; 1.3 pg/ml, 9.0% and 9.0% for IL-1; 1.3 pg/ml, 7.0% and 10.0% for IL-6; 0.16 ng/ml, 5.1% and 7.4% for leptine; 0.78 ng/ml, 0.9% and 2.4% for adiponectine; 1.3 pg/ml, 6.6% and 10% for active PAI-1 and total PAI-1. Insulin resistance was estimated by calculating of the homeostasis model assessment-insulin resistance (HOMA-IR) index (fasting plasma glucose x fasting plasma insulin)/22.5. Bone metabolism markers included the serum concentration of osteocalcin, which was assayed by ELISA (N-MID Osteocalcin ELISA, Nordic Bioscience Diagnostics A/S, Denmark). Intra- and interassay CVs were 2.6% and 4.7%, respectively, with a sensitivity of 0.5 ng/ml. Other bone metabolism markers were Procollagen type I N-terminal propeptide (PINP) and type I-C telopeptide breakdown products (CTX) which were assayed using Cobas 6000 (Roche Diagnostic, Mannheim, Germany) with intra and inter-assay CVs lower than 7%.</p></sec><sec id="sec013"><title>Exercise and nutrition</title><p>Three-day self-report questionnaires on food intake and physical activity allowed the calculation of daily energy intake and daily energy expenditure, as well as daily calcium and protein intake. Routine medications were recorded and all participants were advised to maintain their baseline medications.</p></sec></sec><sec id="sec014"><title>Time of measurements</title><p>Outcomes were measured at baseline (Day 0), 21 days (D21), 3 months (D90), 6 months (D180) and 12 months (D360), with the exception of bone biomarkers which were measured at D0, D21, and D180. Self-reported questionnaires on food intake and physical activity were completed each month.</p></sec><sec id="sec015"><title>Randomization</title><p>Stratified computer-generated randomization (combined with permuted blocks to produce balanced allocation within each stratum) was used to account for sex, age, and body mass index, then participants were assigned to one of the following groups:</p><list list-type="simple"><list-item><p>- <italic>Re</italic>: high-Resistance-moderate-endurance, 10 repetitions performed at 70% of one maximal repetition for resistance and 30% of VO<sub>2</sub>-peak for endurance training,</p></list-item><list-item><p>- <italic>rE</italic>: moderate-resistance (30%)-high-Endurance (70%),</p></list-item><list-item><p>- <italic>re</italic>: moderate-resistance (30%)-moderate-endurance (30%).</p></list-item></list><p>Sex was incorporated as a dummy coded variable. Baseline age was divided at the median (≤60.8 years and >60.8 years) and BMI at the tertiles (≤30.6 kg.m<sup>-2</sup>, 30.7 to 36.6 kg.m<sup>-2</sup>, and >36.6 kg.m<sup>-2</sup>) were also applied to stratification. Based on the number and levels of stratification factors, 12 strata were considered with ≤1% risk of a value smaller than 3 participants per stratum [<xref rid="pone.0136491.ref026" ref-type="bibr">26</xref>].</p><p>All participants followed the same restrictive diet. Assessors for all outcomes were blinded to the participants’ group assignment. All outcome data remained blinded until the completion of the intervention.</p></sec><sec id="sec016"><title>First stage of intervention: A 3-week residential program</title><p>Each day, throughout the residential program, the patients received both standard and personalized balanced meals prescribed by dieticians. Protein accounted for 15 to 20% of the total energy intake (1.2 g.kg<sup>-1</sup>.day<sup>-1</sup> to maintain protein homeostasis) [<xref rid="pone.0136491.ref027" ref-type="bibr">27</xref>], 30 to 35% were lipids, and carbohydrates comprised the remaining composition of the food groups. Participants’ total daily food intake was calculated to enable them to reach a negative energy balance of ~2090 kJ per day (i.e. 500 kcal per day). Participants were individually coached on daily basis, within the context of their assigned group. An equal amount of time (15–20 h/week) was spent by all groups in endurance (90 min daily) and in resistance (90 min, four days a week). Exercises differed only in intensity, from 30% to 70%. Participants’ heart rates were monitored by Polar S810 with instantaneous recording and storage of heart rate values. Endurance training included aquagym, cycling and walking. Resistance training consisted of 8 exercises with free weights and traditional muscle building equipment. Each exercise was performed for three sets of 10 repetitions.</p></sec><sec id="sec017"><title>Second stage of intervention: A 1-year at-home follow-up</title><p>From D21 to D360, the participants were required to complete the same training program without supervision. At D90, D180 and D360, the participants were seen by the dietician and a physical coach for individual adjustments to their program.</p><p>A compliance score was determined on the basis of the number of food questionnaires returned (score from 0 to 12 i.e. 12 = 100%) and the number of training sessions undertaken per week (score from 0 to 4, i.e. 4 = 100%). The overall compliance score was the mean of these two scores (nutrition and physical activity). The participants who reached a score of 60% and over were considered as compliant.</p></sec><sec id="sec018"><title>Statistical analysis</title><p>The number of participants for each group was based upon the amount of expected central fat loss [<xref rid="pone.0136491.ref023" ref-type="bibr">23</xref>]. A sample of 30 participants per intervention group allowed a statistical power greater than 80% with an α level less than 5%, allowing a dropout rate of 20%.</p><p>Descriptive results are presented as means ± standard deviation (SD) unless otherwise noted. The Gaussian distribution for each parameter was assessed by a Shapiro-Wilk test. In case of non-normal distribution, data were log-transformed for analysis. Baseline and follow-up characteristics were cross-sectionally compared between groups using analysis of variance (ANOVA). Alpha levels were set at 0.05 and were adjusted using the Bonferroni technique for multiple comparisons; for example, for 10 comparisons, the α corrected for experiment-wise error rate would be 0.05/10 = 0.005. All these routine analyses were assessed using SPSS software v19.0 (IBM Company, NY, USA).</p><p>For the analysis of the change, taking into account the multilevel design of the program [level 1 units (individuals stratified by age, sex and body mass index) within each level 2 unit (individuals of different MetS groups)], hierarchical random effects models (REM) were constructed using a multilevel modeling approach (MLwiN v2.26, Center for Multilevel Modelling, University of Bristol, Bristol, UK) [<xref rid="pone.0136491.ref028" ref-type="bibr">28</xref>]. Analysis models that contained variables measured at different levels of a hierarchy are known as multilevel regression models. The following additive polynomial multilevel model was adopted to describe the changes in BMC and in BMD from study entry:
<disp-formula id="pone.0136491.e001"><alternatives><graphic xlink:href="pone.0136491.e001.jpg" id="pone.0136491.e001g" position="anchor" mimetype="image" orientation="portrait"></graphic><mml:math id="M1"><mml:mrow><mml:msub><mml:mtext>y</mml:mtext><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.1em"></mml:mspace><mml:mo>=</mml:mo><mml:mspace width="0.25em"></mml:mspace><mml:mo>(</mml:mo><mml:mi mathvariant="normal">α</mml:mi><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">μ</mml:mi><mml:mi>j</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>(</mml:mo><mml:mi mathvariant="normal">β</mml:mi><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi>v</mml:mi><mml:mi>j</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi>x</mml:mi><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>(</mml:mo><mml:msub><mml:mi mathvariant="normal">ɀ</mml:mi><mml:mrow><mml:mn>1</mml:mn><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">ɀ</mml:mi><mml:mrow><mml:mn>2</mml:mn><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>⋯</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">ɀ</mml:mi><mml:mrow><mml:mi>n</mml:mi><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mo>)</mml:mo><mml:mspace width="0.25em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">ɛ</mml:mi><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math></alternatives></disp-formula></p><p>This equation is an example of REM in which level 1 regression coefficients are treated as random variables at level 2. In this example, the number of days from baseline (<italic>x</italic>) is in both the fixed and random parts of the model. This is seen clearly when equation 1 is rearranged into fixed and random parts:
<disp-formula id="pone.0136491.e002"><alternatives><graphic xlink:href="pone.0136491.e002.jpg" id="pone.0136491.e002g" position="anchor" mimetype="image" orientation="portrait"></graphic><mml:math id="M2"><mml:mrow><mml:msub><mml:mtext>y</mml:mtext><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>=</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mrow><mml:mo>(</mml:mo><mml:mi mathvariant="normal">α</mml:mi><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">β</mml:mi><mml:mi>j</mml:mi></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi>x</mml:mi><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>)</mml:mo></mml:mrow><mml:mo>+</mml:mo><mml:mspace width="0.25em"></mml:mspace><mml:mrow><mml:mo>(</mml:mo><mml:msub><mml:mi mathvariant="normal">ɀ</mml:mi><mml:mrow><mml:mn>1</mml:mn><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">ɀ</mml:mi><mml:mrow><mml:mn>2</mml:mn><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>⋯</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">ɀ</mml:mi><mml:mrow><mml:mi>n</mml:mi><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mo>)</mml:mo></mml:mrow><mml:mo>+</mml:mo><mml:mspace width="0.25em"></mml:mspace><mml:mo>(</mml:mo><mml:msub><mml:mi mathvariant="normal">μ</mml:mi><mml:mi>j</mml:mi></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi>v</mml:mi><mml:mi>j</mml:mi></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi>x</mml:mi><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mspace width="0.15em"></mml:mspace><mml:mo>+</mml:mo><mml:mspace width="0.15em"></mml:mspace><mml:msub><mml:mi mathvariant="normal">ɛ</mml:mi><mml:mrow><mml:mi>i</mml:mi><mml:mi>j</mml:mi></mml:mrow></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></alternatives></disp-formula>where <italic>y</italic> is the bone parameter [BMC (g) or BMD (g/cm<sup>2</sup>)] on measurement occasion <italic>i</italic> in the <italic>j</italic>th individual, α is the constant for each <italic>j</italic>th individual, β<sub><italic>j</italic></sub><italic>x</italic><sub><italic>ij</italic></sub> is the slope for the bone parameter over time (in these models, the number of days from baseline was centered around day 180, the middle time point of the study) for the <italic>j</italic>th individual; and ɀ<sub><italic>1</italic></sub> to ɀ<sub><italic>n</italic></sub> were the coefficients of explanatory variables (e.g., intervention group, total body lean mass, vitamin D, etc.) at assessment occasion <italic>i</italic> in the <italic>j</italic>th individual [<xref rid="pone.0136491.ref028" ref-type="bibr">28</xref>,<xref rid="pone.0136491.ref029" ref-type="bibr">29</xref>]. These were the fixed parameters in the model.</p><p>Both μ<sub><italic>j</italic></sub>, <italic>v</italic><sub><italic>j</italic></sub>x<sub><italic>ij</italic></sub> and ε<sub><italic>ij</italic></sub> formed the random parameters in the model. They were assumed to be independent and follow a normal distribution, with means equal to zero and variance σ<sup>2</sup>. ε<sub><italic>ij</italic></sub>~<italic>N</italic>[0,var(ε<sub><italic>ij</italic></sub>)] was the level 1 residual (within-individual variance) for the <italic>i</italic>th assessment of BMC (g) or BMD (g/cm<sup>2</sup>) in the <italic>j</italic>th individual. Also, <italic>μ</italic><sub><italic>j</italic></sub>~<italic>N</italic>[0,var(<italic>μ</italic>)] was the between-individuals’ intercept variance and <italic>v</italic><sub><italic>ij</italic></sub><italic>x</italic><sub><italic>ij</italic></sub>~<italic>N</italic>[0,var(<italic>v</italic><sub><italic>ij</italic></sub><italic>x</italic><sub><italic>ij</italic></sub>)] was the between-individuals’ slope variance; thus being used as the level 2 residuals (between subjects) variances for the <italic>j</italic>th individual. The equation <italic>μ</italic><sub><italic>j</italic></sub>×<italic>v</italic><sub><italic>ij</italic></sub><italic>x</italic><sub><italic>ij</italic></sub>~<italic>N</italic>[0,var(<italic>μ</italic><sub><italic>j</italic></sub>×<italic>v</italic><sub><italic>ij</italic></sub><italic>x</italic><sub><italic>ij</italic></sub>)] explained the intercept-slope covariance relationships among the intercepts and slopes in the model [<xref rid="pone.0136491.ref028" ref-type="bibr">28</xref>,<xref rid="pone.0136491.ref029" ref-type="bibr">29</xref>].</p><p>Models were built using a stepwise procedure, i.e. predictor variables (<italic>z</italic> fixed effects) were added one at a time, and likelihood ratio statistics were used to judge the statistical fit of the model [<xref rid="pone.0136491.ref028" ref-type="bibr">28</xref>]. Predictor variables (<italic>z</italic>) were accepted as significant if the estimated mean coefficient was greater than twice the standard error of the estimate. If the retention criterion was not met, the predictor variable was discarded. Power functions for the number of days from baseline were introduced into the linear models to allow for the nonlinearity of changes in bone parameters. Based on analytical [i.e., Pearson’s product moment correlation coefficients (<italic>r</italic><sub><italic>y</italic>,<italic>x</italic></sub>) between bone parameters (<italic>y</italic>) and potential predictors (<italic>x</italic>) at baseline, D21, D90, D180 and D360] and biological assumptions, the following variables were considered in the multilevel models: days from baseline, days from baseline<sup>2</sup>, age at study start, total body lean mass, total body fat mass, central fat mass, energy intake, protein, calcium, vitamin D, CRP, hsCRP and leptin. Dummy variables were created for MetS, sex and compliance groups with <italic>re</italic> participants, female participants and non-compliant participants as the respective reference categories. Neither insulin, HOMA-IR, pro-inflammatory cytokines (TNF-alpha, IL-1 and IL-6), adiponectin, active and total PAI-1, nor bone metabolism markers (osteocalcin, PINP, and CTX) were significantly related with bone parameters at any measurement occasion, thus they were discarded from the multilevel analysis. A tolerance > 0.10 and a variance inflation factor < 10 were set to avoid collinearity between explanatory variables [<xref rid="pone.0136491.ref030" ref-type="bibr">30</xref>]. A total of six independent multilevel REMs were constructed, specifically: whole body BMC, lumbar spine BMC, femoral neck BMC, whole body BMD, lumbar spine BMD, and femoral neck BMD.</p></sec></sec><sec sec-type="results" id="sec019"><title>Results</title><sec id="sec020"><title>Participants characteristics at baseline</title><p>For the intervention groups, a total of 100 volunteers underwent randomization. Eligible participants were symptomatic of MetS according to the biological tests on D1. Data were retained for analyses only when complete data on primary and secondary outcomes were available. Accordingly, complete data were available for 90 participants at baseline and 71 (79%) completed the whole intervention (<xref rid="pone.0136491.g001" ref-type="fig">Fig 1</xref>). No difference in descriptive characteristics, including bones, was found between participants who dropped out of the program and those who completed it. No significant differences were observed in baseline bone parameters between groups (<xref rid="pone.0136491.t001" ref-type="table">Table 1</xref>). However, <italic>rE</italic> were younger and heavier than <italic>Re</italic> and had higher levels of vitamin D than <italic>re</italic> and <italic>Re</italic>. No differences in baseline lumbar spine BMC and in BMD parameters were observed between the sexes either within or between groups.</p><fig id="pone.0136491.g001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.g001</object-id><label>Fig 1</label><caption><title>Flow chart of participants.</title><p><italic>re</italic>: moderate-resistance-moderate-endurance; <italic>Re</italic>: high-Resistance-moderate-endurance; <italic>rE</italic>: moderate-resistance-high-Endurance.</p></caption><graphic xlink:href="pone.0136491.g001"></graphic></fig><table-wrap id="pone.0136491.t001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.t001</object-id><label>Table 1</label><caption><title>Baseline descriptive characteristics of the intervention groups and controls.</title></caption><alternatives><graphic id="pone.0136491.t001g" xlink:href="pone.0136491.t001"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th rowspan="2" align="left" colspan="1"></th><th align="left" rowspan="1" colspan="1">All <italic>i</italic>-groups</th><th colspan="3" align="center" rowspan="1"><italic>i</italic>-groups</th><th align="left" rowspan="1" colspan="1"><italic>P</italic>-value</th><th align="left" rowspan="1" colspan="1">Control</th><th colspan="2" align="left" rowspan="1">Control <italic>vs</italic>. <italic>i</italic>-groups</th></tr><tr><th align="left" rowspan="1" colspan="1">(<italic>n</italic> = 90)</th><th align="left" rowspan="1" colspan="1"><italic>re</italic> (<italic>n</italic> = 33)</th><th align="left" rowspan="1" colspan="1"><italic>Re</italic> (<italic>n</italic> = 30)</th><th align="left" rowspan="1" colspan="1"><italic>rE</italic> (<italic>n</italic> = 27)</th><th align="left" rowspan="1" colspan="1"><italic>i</italic>-groups</th><th align="left" rowspan="1" colspan="1">(<italic>n</italic> = 44)</th><th align="left" rowspan="1" colspan="1">P-value</th><th align="left" rowspan="1" colspan="1"><italic>Post-hoc</italic></th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Age (years)</td><td align="left" rowspan="1" colspan="1">60.50 ± 5.19</td><td align="left" rowspan="1" colspan="1">60.66±5.49</td><td align="left" rowspan="1" colspan="1">62.18±4.52</td><td align="left" rowspan="1" colspan="1">58.43±4.97</td><td align="left" rowspan="1" colspan="1">0.022 <xref rid="t001fn002" ref-type="table-fn"><sup>a</sup></xref></td><td align="left" rowspan="1" colspan="1">57.89±4.78</td><td align="left" rowspan="1" colspan="1">0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Sex (F/M–<italic>n</italic>)</td><td align="left" rowspan="1" colspan="1">52/38</td><td align="left" rowspan="1" colspan="1">21/12</td><td align="left" rowspan="1" colspan="1">18/12</td><td align="left" rowspan="1" colspan="1">13/14</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">21/23</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Height (cm)</td><td align="left" rowspan="1" colspan="1">164.9 ± 8.6</td><td align="left" rowspan="1" colspan="1">163.8±8.9</td><td align="left" rowspan="1" colspan="1">163.5±6.7</td><td align="left" rowspan="1" colspan="1">167.9±9.7</td><td align="left" rowspan="1" colspan="1">0.106</td><td align="left" rowspan="1" colspan="1">170.2±8.0</td><td align="left" rowspan="1" colspan="1">0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> > <italic>re</italic>, <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Weight (kg)</td><td align="left" rowspan="1" colspan="1">91.3 ± 13.0</td><td align="left" rowspan="1" colspan="1">91.4±12.7</td><td align="left" rowspan="1" colspan="1">87.1±11.8</td><td align="left" rowspan="1" colspan="1">95.9±13.4</td><td align="left" rowspan="1" colspan="1">0.037 <xref rid="t001fn002" ref-type="table-fn"><sup>a</sup></xref></td><td align="left" rowspan="1" colspan="1">69.2±12.1</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Total body lean mass (kg)</td><td align="left" rowspan="1" colspan="1">60.3 ± 10.9</td><td align="left" rowspan="1" colspan="1">58.8±11.2</td><td align="left" rowspan="1" colspan="1">58.7±10.1</td><td align="left" rowspan="1" colspan="1">64.0±11.1</td><td align="left" rowspan="1" colspan="1">0.117</td><td align="left" rowspan="1" colspan="1">50.8±11.0</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Total body fat mass (kg)</td><td align="left" rowspan="1" colspan="1">31.0 ± 7.8</td><td align="left" rowspan="1" colspan="1">32.5±7.6</td><td align="left" rowspan="1" colspan="1">28.4±7.7</td><td align="left" rowspan="1" colspan="1">31.9±7.7</td><td align="left" rowspan="1" colspan="1">0.080</td><td align="left" rowspan="1" colspan="1">16.1±3.9</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Central fat mass (kg)</td><td align="left" rowspan="1" colspan="1">3.1 ± 0.7</td><td align="left" rowspan="1" colspan="1">3.1±0.7</td><td align="left" rowspan="1" colspan="1">3.0±0.7</td><td align="left" rowspan="1" colspan="1">3.1±0.7</td><td align="left" rowspan="1" colspan="1">0.638</td><td align="left" rowspan="1" colspan="1">1.2±0.6</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Energy intake (KJ.d<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">7590.9 ± 2105.4</td><td align="left" rowspan="1" colspan="1">7095.2±1643.0</td><td align="left" rowspan="1" colspan="1">7797.4±1869.9</td><td align="left" rowspan="1" colspan="1">7967.4±2728.1</td><td align="left" rowspan="1" colspan="1">0.227</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Protein (g.d<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">79.7 ± 21.0</td><td align="left" rowspan="1" colspan="1">73.7±16.6</td><td align="left" rowspan="1" colspan="1">80.3±18.3</td><td align="left" rowspan="1" colspan="1">86.4±26.4</td><td align="left" rowspan="1" colspan="1">0.063</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Calcium (mg.d<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">714.2 ± 296.4</td><td align="left" rowspan="1" colspan="1">661.4±193.8</td><td align="left" rowspan="1" colspan="1">781.4±360.4</td><td align="left" rowspan="1" colspan="1">704.0±317.5</td><td align="left" rowspan="1" colspan="1">0.272</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Vitamin D (ng.ml<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">16.93 ± 8.05</td><td align="left" rowspan="1" colspan="1">14.72±5.41</td><td align="left" rowspan="1" colspan="1">15.76±5.03</td><td align="left" rowspan="1" colspan="1">20.94±11.54</td><td align="left" rowspan="1" colspan="1">0.006 <xref rid="t001fn002" ref-type="table-fn"><sup>a</sup></xref><sup>,</sup><xref rid="t001fn003" ref-type="table-fn"><sup>b</sup></xref></td><td align="left" rowspan="1" colspan="1">21.24±6.98</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> > <italic>re</italic>, <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">C-reactive protein (mg.l<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">5.25 ± 3.42</td><td align="left" rowspan="1" colspan="1">6.06±4.07</td><td align="left" rowspan="1" colspan="1">4.54±2.83</td><td align="left" rowspan="1" colspan="1">5.04±3.02</td><td align="left" rowspan="1" colspan="1">0.197</td><td align="left" rowspan="1" colspan="1">3.06±0.74</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">hs C-reactive protein (mg.l<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">4.57 ± 3.91</td><td align="left" rowspan="1" colspan="1">5.24±4.57</td><td align="left" rowspan="1" colspan="1">4.00±3.39</td><td align="left" rowspan="1" colspan="1">4.40±3.56</td><td align="left" rowspan="1" colspan="1">0.441</td><td align="left" rowspan="1" colspan="1">1.25±1.49</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Leptin (ng.dl<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1">3.06 ± 1.57</td><td align="left" rowspan="1" colspan="1">3.44±1.68</td><td align="left" rowspan="1" colspan="1">2.74±1.44</td><td align="left" rowspan="1" colspan="1">2.95±1.53</td><td align="left" rowspan="1" colspan="1">0.195</td><td align="left" rowspan="1" colspan="1">1.21±1.08</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1"><italic>C</italic> < <italic>re</italic>, <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Bone variables</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Total body BMC (g)</td><td align="left" rowspan="1" colspan="1">2259.67 ± 392.57</td><td align="left" rowspan="1" colspan="1">2207.40±380.06</td><td align="left" rowspan="1" colspan="1">2229.05±381.93</td><td align="left" rowspan="1" colspan="1">2357.57±415.38</td><td align="left" rowspan="1" colspan="1">0.297</td><td align="left" rowspan="1" colspan="1">2356.07±459.52</td><td align="left" rowspan="1" colspan="1">0.291</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Lumbar spine BMC (g)</td><td align="left" rowspan="1" colspan="1">67.05 ± 15.45</td><td align="left" rowspan="1" colspan="1">66.58±16.43</td><td align="left" rowspan="1" colspan="1">65.88±14.81</td><td align="left" rowspan="1" colspan="1">68.93±15.32</td><td align="left" rowspan="1" colspan="1">0.745</td><td align="left" rowspan="1" colspan="1">69.80±16.36</td><td align="left" rowspan="1" colspan="1">0.692</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Femoral neck BMC (g)</td><td align="left" rowspan="1" colspan="1">4.27 ± 0.72</td><td align="left" rowspan="1" colspan="1">4.21±0.67</td><td align="left" rowspan="1" colspan="1">4.22±0.73</td><td align="left" rowspan="1" colspan="1">4.41±0.77</td><td align="left" rowspan="1" colspan="1">0.511</td><td align="left" rowspan="1" colspan="1">4.25±0.94</td><td align="left" rowspan="1" colspan="1">0.770</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Total body BMD (g.cm<sup>-2</sup>)</td><td align="left" rowspan="1" colspan="1">1.13 ± 0.10</td><td align="left" rowspan="1" colspan="1">1.12±0.09</td><td align="left" rowspan="1" colspan="1">1.14±0.11</td><td align="left" rowspan="1" colspan="1">1.15±0.11</td><td align="left" rowspan="1" colspan="1">0.618</td><td align="left" rowspan="1" colspan="1">1.16±0.12</td><td align="left" rowspan="1" colspan="1">0.583</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Lumbar spine BMD (g.cm<sup>-2</sup>)</td><td align="left" rowspan="1" colspan="1">1.05 ± 0.17</td><td align="left" rowspan="1" colspan="1">1.05±0.18</td><td align="left" rowspan="1" colspan="1">1.04±0.15</td><td align="left" rowspan="1" colspan="1">1.07±0.16</td><td align="left" rowspan="1" colspan="1">0.790</td><td align="left" rowspan="1" colspan="1">1.03±0.17</td><td align="left" rowspan="1" colspan="1">0.758</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Femoral neck BMD (g.cm<sup>-2</sup>)</td><td align="left" rowspan="1" colspan="1">0.81 ± 0.11</td><td align="left" rowspan="1" colspan="1">0.82±0.11</td><td align="left" rowspan="1" colspan="1">0.79±0.12</td><td align="left" rowspan="1" colspan="1">0.83±0.11</td><td align="left" rowspan="1" colspan="1">0.411</td><td align="left" rowspan="1" colspan="1">0.78±0.14</td><td align="left" rowspan="1" colspan="1">0.234</td><td align="left" rowspan="1" colspan="1">−</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t001fn001"><p>Abbreviations: <italic>i-</italic>groups, intervention groups; <italic>re</italic>, moderate-resistance-moderate-endurance; <italic>Re</italic>, high-Resistance-moderate-endurance; <italic>rE</italic>, moderate-resistance-high-Endurance; <italic>C</italic>, control; F, females; M, males; hs, high-sensitivity; BMC, bone mineral content; BMD, bone mineral density.</p></fn><fn id="t001fn002"><p><sup>a</sup><italic>Re</italic> significantly different than <italic>rE</italic> (<italic>P</italic><0.05).</p></fn><fn id="t001fn003"><p><sup>b</sup><italic>re</italic> significantly different than <italic>rE</italic>. (<italic>P</italic><0.05). <italic>re</italic> did not significantly differ from <italic>Re</italic>.</p></fn></table-wrap-foot></table-wrap><p>The control group (44 participants with no MetS parameters) was matched with the overall MetS group for age and sex. No differences in bone variables between the controls and individuals with MetS were found. However, controls were significantly taller than <italic>re</italic> and <italic>Re</italic>, with less weight, total lean body mass, fat mass, and central fat mass than <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic>. Controls also had higher levels of vitamin D than <italic>re</italic> and <italic>Re</italic> and lower levels of C-reactive protein (CRP), high-sensitivity CRP (hsCRP) and leptin than <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic> (<xref rid="pone.0136491.t001" ref-type="table">Table 1</xref>).</p></sec><sec id="sec021"><title>Cross-sectional MetS-related variation during follow-up</title><p>The descriptive characteristics for the <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic> groups from D21 until D360 are presented in <xref rid="pone.0136491.t002" ref-type="table">Table 2</xref>. Cross sectional analyses revealed that at D21 <italic>Re</italic> had significantly lower body mass than <italic>rE</italic> and less total body fat mass and central fat mass than <italic>re</italic>. Also, <italic>re</italic> had lower energy intake than <italic>rE</italic>. At D90, <italic>re</italic> maintained significantly higher levels of total body fat and central fat mass than <italic>Re</italic>. At D180, <italic>re</italic> had significantly lower energy intake, protein, vitamin D and leptin than <italic>rE</italic>. At D360, <italic>re</italic> had higher total body fat mass than <italic>Re</italic>, while consuming less energy than <italic>rE</italic> and less protein than <italic>Re</italic> and <italic>rE</italic> (<xref rid="pone.0136491.t002" ref-type="table">Table 2</xref>).</p><table-wrap id="pone.0136491.t002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.t002</object-id><label>Table 2</label><caption><title>Descriptive statistics of anthropometric, body composition, food intake, biochemical data and bone variables for the intervention groups, from day 21 to day 360.</title></caption><alternatives><graphic id="pone.0136491.t002g" xlink:href="pone.0136491.t002"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th rowspan="2" align="left" colspan="1"></th><th align="left" rowspan="1" colspan="1">All <italic>i</italic>-groups</th><th colspan="3" align="center" rowspan="1"><italic>i</italic>-groups</th><th align="left" rowspan="1" colspan="1">P-value</th><th align="left" rowspan="1" colspan="1"><italic>Post-hoc</italic></th></tr><tr><th align="left" rowspan="1" colspan="1">(<italic>n</italic> = 325)</th><th align="left" rowspan="1" colspan="1"><italic>re</italic> (<italic>n</italic> = 126)</th><th align="left" rowspan="1" colspan="1"><italic>Re</italic> (<italic>n</italic> = 105)</th><th align="left" rowspan="1" colspan="1"><italic>rE</italic> (<italic>n</italic> = 94)</th><th align="left" rowspan="1" colspan="1"></th><th align="left" rowspan="1" colspan="1"></th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Age (years)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">60.56±5.20</td><td align="left" rowspan="1" colspan="1">60.72±5.49</td><td align="left" rowspan="1" colspan="1">62.24±4.52</td><td align="left" rowspan="1" colspan="1">58.49±4.97</td><td align="left" rowspan="1" colspan="1">0.022</td><td align="left" rowspan="1" colspan="1"><italic>Re > rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">60.78±5.21</td><td align="left" rowspan="1" colspan="1">61.04±5.53</td><td align="left" rowspan="1" colspan="1">62.17±4.47</td><td align="left" rowspan="1" colspan="1">58.76±5.09</td><td align="left" rowspan="1" colspan="1">0.057</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">61.06±5.02</td><td align="left" rowspan="1" colspan="1">61.57±5.38</td><td align="left" rowspan="1" colspan="1">62.33±4.41</td><td align="left" rowspan="1" colspan="1">58.86±4.68</td><td align="left" rowspan="1" colspan="1">0.038</td><td align="left" rowspan="1" colspan="1"><italic>Re > rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">61.69±4.88</td><td align="left" rowspan="1" colspan="1">61.82±5.51</td><td align="left" rowspan="1" colspan="1">63.48±3.81</td><td align="left" rowspan="1" colspan="1">59.41±4.16</td><td align="left" rowspan="1" colspan="1">0.026</td><td align="left" rowspan="1" colspan="1"><italic>Re > rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Sex (F/M–<italic>n</italic>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">52/38</td><td align="left" rowspan="1" colspan="1">21/12</td><td align="left" rowspan="1" colspan="1">18/12</td><td align="left" rowspan="1" colspan="1">13/14</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">48/35</td><td align="left" rowspan="1" colspan="1">21/11</td><td align="left" rowspan="1" colspan="1">14/12</td><td align="left" rowspan="1" colspan="1">13/12</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">48/33</td><td align="left" rowspan="1" colspan="1">21/10</td><td align="left" rowspan="1" colspan="1">15/12</td><td align="left" rowspan="1" colspan="1">12/11</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">39/32</td><td align="left" rowspan="1" colspan="1">20/10</td><td align="left" rowspan="1" colspan="1">11/11</td><td align="left" rowspan="1" colspan="1">8/11</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Compliance (Non-CP/CP–<italic>n</italic>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">27/63</td><td align="left" rowspan="1" colspan="1">11/22</td><td align="left" rowspan="1" colspan="1">7/23</td><td align="left" rowspan="1" colspan="1">9/18</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">24/59</td><td align="left" rowspan="1" colspan="1">11/21</td><td align="left" rowspan="1" colspan="1">6/20</td><td align="left" rowspan="1" colspan="1">7/18</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">23/58</td><td align="left" rowspan="1" colspan="1">11/20</td><td align="left" rowspan="1" colspan="1">6/21</td><td align="left" rowspan="1" colspan="1">6/17</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">17/54</td><td align="left" rowspan="1" colspan="1">10/20</td><td align="left" rowspan="1" colspan="1">5/17</td><td align="left" rowspan="1" colspan="1">3/16</td><td align="left" rowspan="1" colspan="1">−</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Height (cm)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">165.2±8.9</td><td align="left" rowspan="1" colspan="1">163.9±8.9</td><td align="left" rowspan="1" colspan="1">164.1±7.6</td><td align="left" rowspan="1" colspan="1">168.0±9.8</td><td align="left" rowspan="1" colspan="1">0.143</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">165.1±9.0</td><td align="left" rowspan="1" colspan="1">163.8±9.1</td><td align="left" rowspan="1" colspan="1">164.5±8.1</td><td align="left" rowspan="1" colspan="1">167.3±9.7</td><td align="left" rowspan="1" colspan="1">0.309</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">164.7±8.6</td><td align="left" rowspan="1" colspan="1">163.0±8.0</td><td align="left" rowspan="1" colspan="1">164.5±7.9</td><td align="left" rowspan="1" colspan="1">167.3±9.8</td><td align="left" rowspan="1" colspan="1">0.188</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">164.9±8.9</td><td align="left" rowspan="1" colspan="1">163.3±8.6</td><td align="left" rowspan="1" colspan="1">164.6±8.7</td><td align="left" rowspan="1" colspan="1">167.8±9.5</td><td align="left" rowspan="1" colspan="1">0.228</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Weight (kg)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">87.9±12.3</td><td align="left" rowspan="1" colspan="1">88.2±12.1</td><td align="left" rowspan="1" colspan="1">83.7±11.3</td><td align="left" rowspan="1" colspan="1">92.0±12.4</td><td align="left" rowspan="1" colspan="1">0.037</td><td align="left" rowspan="1" colspan="1"><italic>Re < rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">84.3±11.9</td><td align="left" rowspan="1" colspan="1">85.3±12.3</td><td align="left" rowspan="1" colspan="1">80.6±10.9</td><td align="left" rowspan="1" colspan="1">86.9±11.7</td><td align="left" rowspan="1" colspan="1">0.133</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">84.5±12.7</td><td align="left" rowspan="1" colspan="1">84.9±12.6</td><td align="left" rowspan="1" colspan="1">81.7±12.4</td><td align="left" rowspan="1" colspan="1">87.2±13.2</td><td align="left" rowspan="1" colspan="1">0.311</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">84.2±12.7</td><td align="left" rowspan="1" colspan="1">85.4±13.1</td><td align="left" rowspan="1" colspan="1">80.3±11.2</td><td align="left" rowspan="1" colspan="1">86.8±13.3</td><td align="left" rowspan="1" colspan="1">0.212</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Total body lean mass (kg)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">59.5±10.5</td><td align="left" rowspan="1" colspan="1">57.8±10.7</td><td align="left" rowspan="1" colspan="1">58.3±9.7</td><td align="left" rowspan="1" colspan="1">62.9±10.6</td><td align="left" rowspan="1" colspan="1">0.130</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">58.2±10.5</td><td align="left" rowspan="1" colspan="1">56.8±11.3</td><td align="left" rowspan="1" colspan="1">57.6±9.8</td><td align="left" rowspan="1" colspan="1">60.5±10.0</td><td align="left" rowspan="1" colspan="1">0.392</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">58.4±10.3</td><td align="left" rowspan="1" colspan="1">56.6±10.8</td><td align="left" rowspan="1" colspan="1">58.3±9.9</td><td align="left" rowspan="1" colspan="1">61.1±10.0</td><td align="left" rowspan="1" colspan="1">0.285</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">58.2±10.9</td><td align="left" rowspan="1" colspan="1">56.5±11.4</td><td align="left" rowspan="1" colspan="1">57.9±10.6</td><td align="left" rowspan="1" colspan="1">61.4±10.2</td><td align="left" rowspan="1" colspan="1">0.309</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Total body fat mass (kg)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">28.3±7.5</td><td align="left" rowspan="1" colspan="1">30.3±7.4</td><td align="left" rowspan="1" colspan="1">25.4±7.2</td><td align="left" rowspan="1" colspan="1">29.1±7.4</td><td align="left" rowspan="1" colspan="1">0.027</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> > <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">26.1±7.3</td><td align="left" rowspan="1" colspan="1">28.5±6.8</td><td align="left" rowspan="1" colspan="1">22.9±7.4</td><td align="left" rowspan="1" colspan="1">26.4±7.0</td><td align="left" rowspan="1" colspan="1">0.015</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> > <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">26.0±8.1</td><td align="left" rowspan="1" colspan="1">28.3±6.8</td><td align="left" rowspan="1" colspan="1">23.4±8.6</td><td align="left" rowspan="1" colspan="1">26.1±8.3</td><td align="left" rowspan="1" colspan="1">0.067</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">25.9±7.7</td><td align="left" rowspan="1" colspan="1">28.8±7.3</td><td align="left" rowspan="1" colspan="1">22.4±6.7</td><td align="left" rowspan="1" colspan="1">25.5±8.0</td><td align="left" rowspan="1" colspan="1">0.010</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> > <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Central fat mass (kg)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">2.7±0.6</td><td align="left" rowspan="1" colspan="1">2.9±0.6</td><td align="left" rowspan="1" colspan="1">2.5±0.6</td><td align="left" rowspan="1" colspan="1">2.7±0.6</td><td align="left" rowspan="1" colspan="1">0.016</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> > <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">2.5±0.6</td><td align="left" rowspan="1" colspan="1">2.7±0.6</td><td align="left" rowspan="1" colspan="1">2.2±0.5</td><td align="left" rowspan="1" colspan="1">2.4±0.6</td><td align="left" rowspan="1" colspan="1">0.036</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> > <italic>Re</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">2.4±0.7</td><td align="left" rowspan="1" colspan="1">2.6±0.7</td><td align="left" rowspan="1" colspan="1">2.2±0.8</td><td align="left" rowspan="1" colspan="1">2.3±0.7</td><td align="left" rowspan="1" colspan="1">0.105</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">2.5±0.7</td><td align="left" rowspan="1" colspan="1">2.7±0.7</td><td align="left" rowspan="1" colspan="1">2.3±0.7</td><td align="left" rowspan="1" colspan="1">2.4±0.8</td><td align="left" rowspan="1" colspan="1">0.066</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Energy intake (KJ.d<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">6534.0±511.4</td><td align="left" rowspan="1" colspan="1">6371.0±572.1</td><td align="left" rowspan="1" colspan="1">6516.4±512.6</td><td align="left" rowspan="1" colspan="1">6752.7±340.1</td><td align="left" rowspan="1" colspan="1">0.014</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> < <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">6704.4±1087.7</td><td align="left" rowspan="1" colspan="1">6510.0±961.7</td><td align="left" rowspan="1" colspan="1">6882.0±1257.2</td><td align="left" rowspan="1" colspan="1">6768.7±1054.6</td><td align="left" rowspan="1" colspan="1">0.411</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">6673.0±965.5</td><td align="left" rowspan="1" colspan="1">6330.2±773.5</td><td align="left" rowspan="1" colspan="1">6831.9±1187.6</td><td align="left" rowspan="1" colspan="1">6948.6±794.4</td><td align="left" rowspan="1" colspan="1">0.036</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> < <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">6588.3±931.6</td><td align="left" rowspan="1" colspan="1">6390.0±821.7</td><td align="left" rowspan="1" colspan="1">6696.6±1073.7</td><td align="left" rowspan="1" colspan="1">6775.9±908.3</td><td align="left" rowspan="1" colspan="1">0.301</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Protein (g.d<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">101.6±11.1</td><td align="left" rowspan="1" colspan="1">99.1±11.7</td><td align="left" rowspan="1" colspan="1">100.7±11.3</td><td align="left" rowspan="1" colspan="1">105.5±9.1</td><td align="left" rowspan="1" colspan="1">0.070</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">84.1±13.4</td><td align="left" rowspan="1" colspan="1">83.4±14.2</td><td align="left" rowspan="1" colspan="1">82.3±10.4</td><td align="left" rowspan="1" colspan="1">86.8±15.1</td><td align="left" rowspan="1" colspan="1">0.464</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">82.2±14.9</td><td align="left" rowspan="1" colspan="1">76.2±16.3</td><td align="left" rowspan="1" colspan="1">81.9±12.4</td><td align="left" rowspan="1" colspan="1">90.7±11.7</td><td align="left" rowspan="1" colspan="1">0.001</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> < <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">81.2±10.6</td><td align="left" rowspan="1" colspan="1">78.8±10.7</td><td align="left" rowspan="1" colspan="1">79.2±10.5</td><td align="left" rowspan="1" colspan="1">87.3±8.3</td><td align="left" rowspan="1" colspan="1">0.011</td><td align="left" rowspan="1" colspan="1"><italic>re</italic>, <italic>Re</italic> < <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1">Calcium (mg.d<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">830.2±103.7</td><td align="left" rowspan="1" colspan="1">835.1±98.1</td><td align="left" rowspan="1" colspan="1">830.3±100.4</td><td align="left" rowspan="1" colspan="1">824.2±116.7</td><td align="left" rowspan="1" colspan="1">0.922</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">769.1±202.9</td><td align="left" rowspan="1" colspan="1">766.8±258.2</td><td align="left" rowspan="1" colspan="1">792.8±159.6</td><td align="left" rowspan="1" colspan="1">747.5±163.9</td><td align="left" rowspan="1" colspan="1">0.730</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">811.3±137.3</td><td align="left" rowspan="1" colspan="1">788.0±140.0</td><td align="left" rowspan="1" colspan="1">802.8±141.8</td><td align="left" rowspan="1" colspan="1">852.8±124.3</td><td align="left" rowspan="1" colspan="1">0.214</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">778.2±129.8</td><td align="left" rowspan="1" colspan="1">776.2±143.2</td><td align="left" rowspan="1" colspan="1">785.4±147.4</td><td align="left" rowspan="1" colspan="1">773.0±84.1</td><td align="left" rowspan="1" colspan="1">0.950</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Vitamin D (ng.ml<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">21.53±17.13</td><td align="left" rowspan="1" colspan="1">19.29±10.19</td><td align="left" rowspan="1" colspan="1">18.49 ± 6.21</td><td align="left" rowspan="1" colspan="1">27.64±27.89</td><td align="left" rowspan="1" colspan="1">0.083</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">18.92±10.62</td><td align="left" rowspan="1" colspan="1">18.88±12.20</td><td align="left" rowspan="1" colspan="1">17.40 ± 5.03</td><td align="left" rowspan="1" colspan="1">20.56±12.69</td><td align="left" rowspan="1" colspan="1">0.572</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">19.31±9.33</td><td align="left" rowspan="1" colspan="1">15.56±4.30</td><td align="left" rowspan="1" colspan="1">21.52 ± 8.78</td><td align="left" rowspan="1" colspan="1">21.76±12.94</td><td align="left" rowspan="1" colspan="1">0.015</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> < <italic>Re</italic>, <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">21.12±6.76</td><td align="left" rowspan="1" colspan="1">20.07±7.29</td><td align="left" rowspan="1" colspan="1">20.64 ± 5.86</td><td align="left" rowspan="1" colspan="1">23.33±6.71</td><td align="left" rowspan="1" colspan="1">0.242</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">C-reactive protein (mg.l<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">4.35±3.09</td><td align="left" rowspan="1" colspan="1">4.86±3.22</td><td align="left" rowspan="1" colspan="1">3.67±3.11</td><td align="left" rowspan="1" colspan="1">4.47±2.88</td><td align="left" rowspan="1" colspan="1">0.308</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">4.93±4.67</td><td align="left" rowspan="1" colspan="1">5.35±4.12</td><td align="left" rowspan="1" colspan="1">5.54±6.70</td><td align="left" rowspan="1" colspan="1">3.74±1.92</td><td align="left" rowspan="1" colspan="1">0.317</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">4.15±2.32</td><td align="left" rowspan="1" colspan="1">4.61±2.83</td><td align="left" rowspan="1" colspan="1">3.85±1.99</td><td align="left" rowspan="1" colspan="1">3.89±1.84</td><td align="left" rowspan="1" colspan="1">0.384</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">3.76±1.69</td><td align="left" rowspan="1" colspan="1">3.91±1.49</td><td align="left" rowspan="1" colspan="1">3.32±1.72</td><td align="left" rowspan="1" colspan="1">4.02±1.94</td><td align="left" rowspan="1" colspan="1">0.346</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">hs C-reactive protein (mg.l<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">3.17±3.66</td><td align="left" rowspan="1" colspan="1">3.86±4.15</td><td align="left" rowspan="1" colspan="1">2.48±3.64</td><td align="left" rowspan="1" colspan="1">3.10±2.95</td><td align="left" rowspan="1" colspan="1">0.331</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">3.94±4.92</td><td align="left" rowspan="1" colspan="1">4.52±4.39</td><td align="left" rowspan="1" colspan="1">4.19±6.93</td><td align="left" rowspan="1" colspan="1">2.96±2.50</td><td align="left" rowspan="1" colspan="1">0.479</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">2.87±2.67</td><td align="left" rowspan="1" colspan="1">3.19±3.13</td><td align="left" rowspan="1" colspan="1">2.33±1.98</td><td align="left" rowspan="1" colspan="1">3.08±2.73</td><td align="left" rowspan="1" colspan="1">0.431</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">2.51±2.49</td><td align="left" rowspan="1" colspan="1">2.76±2.05</td><td align="left" rowspan="1" colspan="1">2.03±2.87</td><td align="left" rowspan="1" colspan="1">2.67±2.73</td><td align="left" rowspan="1" colspan="1">0.560</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Leptin (ng.dl<sup>-1</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">2.41±1.78</td><td align="left" rowspan="1" colspan="1">2.69±1.37</td><td align="left" rowspan="1" colspan="1">2.19±2.05</td><td align="left" rowspan="1" colspan="1">2.31±1.90</td><td align="left" rowspan="1" colspan="1">0.513</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">2.14±1.89</td><td align="left" rowspan="1" colspan="1">2.46±1.51</td><td align="left" rowspan="1" colspan="1">1.66±1.72</td><td align="left" rowspan="1" colspan="1">2.24±2.40</td><td align="left" rowspan="1" colspan="1">0.260</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">2.33±1.89</td><td align="left" rowspan="1" colspan="1">3.00±1.86</td><td align="left" rowspan="1" colspan="1">2.14±2.06</td><td align="left" rowspan="1" colspan="1">1.63±1.46</td><td align="left" rowspan="1" colspan="1">0.024</td><td align="left" rowspan="1" colspan="1"><italic>re</italic> > <italic>rE</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">1.96±1.34</td><td align="left" rowspan="1" colspan="1">2.28±1.41</td><td align="left" rowspan="1" colspan="1">1.81±1.33</td><td align="left" rowspan="1" colspan="1">1.65±1.20</td><td align="left" rowspan="1" colspan="1">0.226</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1">Bone variables</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Total body BMC (g)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">2267.54±391.68</td><td align="left" rowspan="1" colspan="1">2211.90±374.90</td><td align="left" rowspan="1" colspan="1">2234.42±369.07</td><td align="left" rowspan="1" colspan="1">2372.35±428.52</td><td align="left" rowspan="1" colspan="1">0.247</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">2234.77±398.57</td><td align="left" rowspan="1" colspan="1">2196.47±393.54</td><td align="left" rowspan="1" colspan="1">2221.97±378.53</td><td align="left" rowspan="1" colspan="1">2297.09±432.77</td><td align="left" rowspan="1" colspan="1">0.633</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">2227.28±387.18</td><td align="left" rowspan="1" colspan="1">2166.26±381.54</td><td align="left" rowspan="1" colspan="1">2208.52±372.50</td><td align="left" rowspan="1" colspan="1">2331.55±406.92</td><td align="left" rowspan="1" colspan="1">0.290</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">2248.88±391.91</td><td align="left" rowspan="1" colspan="1">2181.22±383.08</td><td align="left" rowspan="1" colspan="1">2236.35±400.50</td><td align="left" rowspan="1" colspan="1">2370.22±387.97</td><td align="left" rowspan="1" colspan="1">0.258</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Lumbar spine BMC (g)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">68.31±16.14</td><td align="left" rowspan="1" colspan="1">67.34±17.18</td><td align="left" rowspan="1" colspan="1">67.24±15.34</td><td align="left" rowspan="1" colspan="1">70.69±16.07</td><td align="left" rowspan="1" colspan="1">0.662</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">67.95±16.33</td><td align="left" rowspan="1" colspan="1">68.22±17.30</td><td align="left" rowspan="1" colspan="1">66.53±15.30</td><td align="left" rowspan="1" colspan="1">69.09±16.64</td><td align="left" rowspan="1" colspan="1">0.852</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">67.50±16.38</td><td align="left" rowspan="1" colspan="1">66.75±17.45</td><td align="left" rowspan="1" colspan="1">66.41±15.72</td><td align="left" rowspan="1" colspan="1">69.77±16.16</td><td align="left" rowspan="1" colspan="1">0.736</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">67.98±16.40</td><td align="left" rowspan="1" colspan="1">66.14±17.58</td><td align="left" rowspan="1" colspan="1">67.10±16.24</td><td align="left" rowspan="1" colspan="1">71.92±14.76</td><td align="left" rowspan="1" colspan="1">0.469</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Femoral neck BMC (g)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">4.32±0.77</td><td align="left" rowspan="1" colspan="1">4.22±0.74</td><td align="left" rowspan="1" colspan="1">4.28±0.73</td><td align="left" rowspan="1" colspan="1">4.51±0.85</td><td align="left" rowspan="1" colspan="1">0.326</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">4.26±0.75</td><td align="left" rowspan="1" colspan="1">4.25±0.79</td><td align="left" rowspan="1" colspan="1">4.24±0.69</td><td align="left" rowspan="1" colspan="1">4.29±0.80</td><td align="left" rowspan="1" colspan="1">0.972</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">4.27±0.74</td><td align="left" rowspan="1" colspan="1">4.25±0.78</td><td align="left" rowspan="1" colspan="1">4.19±0.70</td><td align="left" rowspan="1" colspan="1">4.40±0.75</td><td align="left" rowspan="1" colspan="1">0.581</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">4.21±0.71</td><td align="left" rowspan="1" colspan="1">4.11±0.70</td><td align="left" rowspan="1" colspan="1">4.18±0.71</td><td align="left" rowspan="1" colspan="1">4.37±0.71</td><td align="left" rowspan="1" colspan="1">0.453</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Total body BMD (g.cm<sup>-2</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">1.14±0.10</td><td align="left" rowspan="1" colspan="1">1.12±0.09</td><td align="left" rowspan="1" colspan="1">1.15±0.09</td><td align="left" rowspan="1" colspan="1">1.15±0.11</td><td align="left" rowspan="1" colspan="1">0.437</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">1.14±0.10</td><td align="left" rowspan="1" colspan="1">1.12±0.09</td><td align="left" rowspan="1" colspan="1">1.14±0.10</td><td align="left" rowspan="1" colspan="1">1.15±0.11</td><td align="left" rowspan="1" colspan="1">0.540</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">1.13±0.10</td><td align="left" rowspan="1" colspan="1">1.12±0.10</td><td align="left" rowspan="1" colspan="1">1.13±0.10</td><td align="left" rowspan="1" colspan="1">1.15±0.10</td><td align="left" rowspan="1" colspan="1">0.437</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">1.14±0.10</td><td align="left" rowspan="1" colspan="1">1.12±0.10</td><td align="left" rowspan="1" colspan="1">1.14±0.11</td><td align="left" rowspan="1" colspan="1">1.17±0.11</td><td align="left" rowspan="1" colspan="1">0.412</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Lumbar spine BMD (g.cm<sup>-2</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">1.06±0.17</td><td align="left" rowspan="1" colspan="1">1.05±0.19</td><td align="left" rowspan="1" colspan="1">1.05±0.15</td><td align="left" rowspan="1" colspan="1">1.08±0.17</td><td align="left" rowspan="1" colspan="1">0.729</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">1.05±0.17</td><td align="left" rowspan="1" colspan="1">1.06±0.19</td><td align="left" rowspan="1" colspan="1">1.03±0.14</td><td align="left" rowspan="1" colspan="1">1.07±0.18</td><td align="left" rowspan="1" colspan="1">0.659</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">1.05±0.17</td><td align="left" rowspan="1" colspan="1">1.05±0.20</td><td align="left" rowspan="1" colspan="1">1.03±0.15</td><td align="left" rowspan="1" colspan="1">1.08±0.16</td><td align="left" rowspan="1" colspan="1">0.497</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">1.05±0.17</td><td align="left" rowspan="1" colspan="1">1.05±0.20</td><td align="left" rowspan="1" colspan="1">1.02±0.15</td><td align="left" rowspan="1" colspan="1">1.10±0.15</td><td align="left" rowspan="1" colspan="1">0.402</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> Femoral neck BMD (g.cm<sup>-2</sup>)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> D21</td><td align="left" rowspan="1" colspan="1">0.81±0.12</td><td align="left" rowspan="1" colspan="1">0.82±0.12</td><td align="left" rowspan="1" colspan="1">0.80±0.12</td><td align="left" rowspan="1" colspan="1">0.84±0.12</td><td align="left" rowspan="1" colspan="1">0.363</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D90</td><td align="left" rowspan="1" colspan="1">0.81±0.11</td><td align="left" rowspan="1" colspan="1">0.82±0.11</td><td align="left" rowspan="1" colspan="1">0.78±0.10</td><td align="left" rowspan="1" colspan="1">0.81±0.11</td><td align="left" rowspan="1" colspan="1">0.376</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D180</td><td align="left" rowspan="1" colspan="1">0.80±0.11</td><td align="left" rowspan="1" colspan="1">0.82±0.12</td><td align="left" rowspan="1" colspan="1">0.78±0.09</td><td align="left" rowspan="1" colspan="1">0.82±0.11</td><td align="left" rowspan="1" colspan="1">0.247</td><td align="left" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"> D360</td><td align="left" rowspan="1" colspan="1">0.80±0.11</td><td align="left" rowspan="1" colspan="1">0.80±0.11</td><td align="left" rowspan="1" colspan="1">0.76±0.10</td><td align="left" rowspan="1" colspan="1">0.82±0.11</td><td align="left" rowspan="1" colspan="1">0.188</td><td align="left" rowspan="1" colspan="1">−</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t002fn001"><p>Abbreviations: <italic>i-</italic>groups, intervention groups; <italic>re</italic>, moderate-resistance-moderate-endurance; <italic>Re</italic>, high-Resistance-moderate-endurance; <italic>rE</italic>, moderate-resistance-high-Endurance; <italic>C</italic>, control; M, males; F, females; Non-CP, non-compliant; CP, compliant; hs, high-sensitivity; BMC, bone mineral content; BMD, bone mineral density.</p></fn></table-wrap-foot></table-wrap><p>There were no significant differences among MetS groups for BMC and BMD variables throughout the duration of the study (<xref rid="pone.0136491.t002" ref-type="table">Table 2</xref>). Also, no MetS group differed in bone mass and bone density from controls (<xref rid="pone.0136491.g002" ref-type="fig">Fig 2</xref>).</p><fig id="pone.0136491.g002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.g002</object-id><label>Fig 2</label><caption><title>Changes (360 days) on total body bone mineral content (BMC) and density (BMD), lumbar spine BMC and BMD and femoral neck BMC and BMD for <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic> groups.</title><p><italic>re</italic>: moderate-resistance-moderate-endurance; <italic>Re</italic>: high-Resistance-moderate-endurance; <italic>rE</italic>: moderate-resistance-high-Endurance. There were no significant differences in BMD and BMC parameters between <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic> participants across the intervention. Participants in the intervention did not have significantly greater or lower bone mass or density development than controls.</p></caption><graphic xlink:href="pone.0136491.g002"></graphic></fig></sec><sec id="sec022"><title>Cross-sectional effects of compliance</title><p>Compliance scores did not differ between groups. There was no difference between the diet compliance score and the physical activity compliance score. Overall, compliance score was 71.7±7.2% in compliant participants and 34.4±15.2% in non-compliant participants. This translates to an estimated 14.3±1.4 hours of physical activity in compliant participants and 6.8±3.0 in non-compliant participants.</p><p>Cross-sectional analyses of the compliance effects on BMC and BMD from D21 to D360 are displayed in Figs <xref rid="pone.0136491.g003" ref-type="fig">3</xref> and <xref rid="pone.0136491.g004" ref-type="fig">4</xref>, respectively. At D21, compliant <italic>re</italic> and <italic>Re</italic> participants had significantly more lumbar spine BMC than their less-compliant <italic>re</italic> and <italic>Re</italic> peers. Similar findings were observed in compliant <italic>Re</italic> participants for lumbar spine BMD. Non-compliant <italic>re</italic> participants also had lower lumbar spine BMC than controls. At D90 and D360, compliant re participants maintained significantly higher lumbar spine BMC than non-compliant re participants. At D180 and D360, total body BMC and lumbar spine BMC were significantly lower in non-compliant <italic>re</italic> participants than in controls. At the end of the intervention program, compliant <italic>rE</italic> participants had greater femoral neck BMD than compliant <italic>Re</italic> participants and controls.</p><fig id="pone.0136491.g003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.g003</object-id><label>Fig 3</label><caption><title>Compliance effect (360 days) on total body bone mineral content (BMC), lumbar spine BMC and femoral neck BMC for <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic> groups.</title><p><italic>re</italic>: moderate-resistance-moderate-endurance; <italic>Re</italic>: high-Resistance-moderate-endurance; <italic>rE</italic>: moderate-resistance-high-Endurance. * Compliant participants significantly different from non-compliants (p<0.05). ‡ Non-compliant participants significantly different from controls.</p></caption><graphic xlink:href="pone.0136491.g003"></graphic></fig><fig id="pone.0136491.g004" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.g004</object-id><label>Fig 4</label><caption><title>Compliance effect (360 days) on total body bone mineral density (BMD), lumbar spine BMD and femoral neck BMD for <italic>re</italic>, <italic>Re</italic> and <italic>rE</italic> groups.</title><p><italic>re</italic>: moderate-resistance-moderate-endurance; <italic>Re</italic>: high-Resistance-moderate-endurance; <italic>rE</italic>: moderate-resistance-high-Endurance. *Compliant participants significantly different from non-compliants (p<0.05).</p></caption><graphic xlink:href="pone.0136491.g004"></graphic></fig></sec><sec id="sec023"><title>Longitudinal multilevel modeling analyses</title><p>Tables <xref rid="pone.0136491.t003" ref-type="table">3</xref> and <xref rid="pone.0136491.t004" ref-type="table">4</xref> summarize the results from multilevel models for BMC and BMD at the total body, lumbar spine, and femoral neck. Total body BMC and BMD, lumbar spine BMC and BMD, and femoral neck BMD changed significantly over baseline within individuals at each measurement occasion. However, the three different modalities of exercise did not produce significantly different effects on any BMC or BMD measurements. The significant contribution of sex observed in five multilevel models indicates that female participants, regardless of the MetS group, had significantly less total body BMC (-556.45±60.82 g), lumbar spine BMC (-12.01±3.04 g) femoral neck BMC (-0.70±0.14 g), total body BMD (0.06±0.02 g.cm<sup>-2</sup>) and femoral neck BMD (0.04±0.02 g.cm<sup>-2</sup>) than their respective male counterparts when other confounders were controlled. An independent and significant increment of 8.14±3.27 g, 0.06±0.03 g.cm<sup>-2</sup>, and 0.04±0.02 g.cm<sup>-2</sup> was also estimated for lumbar spine BMC, lumbar spine BMD, and femoral neck BMD, respectively, for those participants who were compliant with the exercise prescription throughout the intervention.</p><table-wrap id="pone.0136491.t003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.t003</object-id><label>Table 3</label><caption><title>Multilevel regression analysis of total body, lumber spine and femoral neck bone mineral content (BMC) aligned by days from study entry.</title></caption><alternatives><graphic id="pone.0136491.t003g" xlink:href="pone.0136491.t003"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><tbody><tr><td align="left" rowspan="1" colspan="1"></td><td colspan="7" align="center" rowspan="1">BMC</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td colspan="2" align="center" rowspan="1">Total body</td><td colspan="3" align="center" rowspan="1">Lumbar spine</td><td colspan="2" align="center" rowspan="1">Femoral neck</td></tr><tr><td align="left" rowspan="1" colspan="1">Fixed explanatory variables</td><td colspan="2" align="center" rowspan="1">Estimates</td><td colspan="3" align="center" rowspan="1">Estimates</td><td colspan="2" align="center" rowspan="1">Estimates</td></tr><tr><td align="left" rowspan="1" colspan="1"> Constant</td><td colspan="2" align="center" rowspan="1">2073.2730±43.1213</td><td colspan="3" align="center" rowspan="1">69.3464±3.0431</td><td colspan="2" align="center" rowspan="1">3.8773±0.1015</td></tr><tr><td align="left" rowspan="1" colspan="1"> Days from start</td><td colspan="2" align="center" rowspan="1">-0.2123±0.0654</td><td colspan="3" align="center" rowspan="1">0.0019±0.0030</td><td colspan="2" align="center" rowspan="1">0.0002±0.0002</td></tr><tr><td align="left" rowspan="1" colspan="1"> Days from start <sup>2</sup></td><td colspan="2" align="center" rowspan="1">0.0004±0.0002</td><td colspan="3" align="center" rowspan="1">-0.000005±0.000008</td><td colspan="2" align="center" rowspan="1">-0.000001±0.0000006</td></tr><tr><td align="left" rowspan="1" colspan="1"> Age at study start</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> re <italic>vs</italic> Re</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> re <italic>vs</italic> rE</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Female <italic>vs</italic> male</td><td colspan="2" align="center" rowspan="1">556.4508±60.8164</td><td colspan="3" align="center" rowspan="1">12.0111±3.0409</td><td colspan="2" align="center" rowspan="1">0.6962±0.1360</td></tr><tr><td align="left" rowspan="1" colspan="1"> Non-compliant <italic>vs</italic> compliant</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">8.1360±3.2744</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Changes in total body lean mass</td><td colspan="2" align="center" rowspan="1">-0.0068± 0.0019</td><td colspan="3" align="center" rowspan="1">-0.0003± 0.0001</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Changes in total body fat mass</td><td colspan="2" align="center" rowspan="1">0.0030±0.0012</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Changes in central fat mass</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Energy intake</td><td colspan="2" align="center" rowspan="1">-0.0044±0.0020</td><td colspan="3" align="center" rowspan="1">-0.0002±0.0001</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Protein</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Calcium</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">0.0001±0.00005</td></tr><tr><td align="left" rowspan="1" colspan="1"> Vitamin D</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">0.0020±0.0009</td><td colspan="2" align="center" rowspan="1">0.0019±0.0009</td></tr><tr><td align="left" rowspan="1" colspan="1"> C-reactive protein</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> high-sensitivity C-reactive protein</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Leptin</td><td colspan="2" align="center" rowspan="1">-0.0004±0.0002</td><td colspan="3" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1">Variance-covariance matrix of random variables</td><td align="center" rowspan="1" colspan="1">Constant</td><td align="center" rowspan="1" colspan="1">Days from start</td><td align="center" rowspan="1" colspan="1">Constant</td><td colspan="2" align="center" rowspan="1">Days from start</td><td align="center" rowspan="1" colspan="1">Constant</td><td align="center" rowspan="1" colspan="1">Days from start</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Level 1 (within individuals)</italic></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td colspan="2" align="center" rowspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Constant</td><td align="center" rowspan="1" colspan="1">1276.48±114.44</td><td align="center" rowspan="1" colspan="1"></td><td colspan="2" align="center" rowspan="1">3.48±0.31</td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">0.0307±0.0652</td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Level 2 (between individuals)</italic></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td colspan="2" align="center" rowspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Constant</td><td align="center" rowspan="1" colspan="1">85896.77±12885.53</td><td align="center" rowspan="1" colspan="1"></td><td colspan="2" align="center" rowspan="1">200.23±30.06</td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">0.4258±0.0652</td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Days from start centered</td><td align="center" rowspan="1" colspan="1">-8.65±4.59</td><td align="center" rowspan="1" colspan="1">0.0022±0.0032</td><td colspan="2" align="center" rowspan="1">0.0078±0.0143</td><td align="center" rowspan="1" colspan="1">0.00004 ± 0.00001</td><td align="center" rowspan="1" colspan="1">-0.00007±0.00004</td><td align="center" rowspan="1" colspan="1">0.00000002±0.00000007</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t003fn001"><p>Abbreviations: <italic>re</italic>, moderate-resistance-moderate-endurance; <italic>Re</italic>, high-Resistance-moderate-endurance; <italic>rE</italic>, moderate-resistance-high-Endurance.</p></fn><fn id="t003fn002"><p>Fixed effect values are presented as estimated mean coefficients ± SEE (standard error of estimate) of BMC in grams. Random effects values presented as estimated mean variance ± SEE (BMC) in grams<sup>2</sup>. Days from start was centered around 180 days. Changes in total body lean mass, total body fat mass and central fat mass (g) from study entry.</p></fn></table-wrap-foot></table-wrap><table-wrap id="pone.0136491.t004" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0136491.t004</object-id><label>Table 4</label><caption><title>Multilevel regression analysis of total body, lumber spine and femoral neck bone mineral density (BMD) aligned by days from baseline.</title></caption><alternatives><graphic id="pone.0136491.t004g" xlink:href="pone.0136491.t004"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><tbody><tr><td align="left" rowspan="1" colspan="1"></td><td colspan="6" align="center" rowspan="1">BMD</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td colspan="2" align="center" rowspan="1">Total body</td><td colspan="2" align="center" rowspan="1">Lumbar spine</td><td colspan="2" align="center" rowspan="1">Femoral neck</td></tr><tr><td align="left" rowspan="1" colspan="1">Fixed explanatory variables</td><td colspan="2" align="center" rowspan="1">Estimates</td><td colspan="2" align="center" rowspan="1">Estimates</td><td colspan="2" align="center" rowspan="1">Estimates</td></tr><tr><td align="left" rowspan="1" colspan="1"> Constant</td><td colspan="2" align="center" rowspan="1">1.1119±0.0138</td><td colspan="2" align="center" rowspan="1">1.0956±0.0319</td><td colspan="2" align="center" rowspan="1">1.0727±0.1260</td></tr><tr><td align="left" rowspan="1" colspan="1"> Days from start</td><td colspan="2" align="center" rowspan="1">-0.000015±0.000006</td><td colspan="2" align="center" rowspan="1">-0.000005±0.0000011</td><td colspan="2" align="center" rowspan="1">-0.000047±0.000008</td></tr><tr><td align="left" rowspan="1" colspan="1"> Days from start <sup>2</sup></td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Age at study start</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">-0.0041± 0.000209</td></tr><tr><td align="left" rowspan="1" colspan="1"> re <italic>vs</italic> Re</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> re <italic>vs</italic> rE</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Female <italic>vs</italic> male</td><td colspan="2" align="center" rowspan="1">0.0608±0.0201</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">0.0440±0.0215</td></tr><tr><td align="left" rowspan="1" colspan="1"> Non-compliant <italic>vs</italic> compliant</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">0.0618±0.0280</td><td colspan="2" align="center" rowspan="1">0.0415±0.0203</td></tr><tr><td align="left" rowspan="1" colspan="1"> Changes in total body lean mass</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Changes in total body fat mass</td><td colspan="2" align="center" rowspan="1">- 0.0000011±0.0000003</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Changes in central fat mass</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Energy intake</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Protein</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Calcium</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Vitamin D</td><td colspan="2" align="center" rowspan="1">0.00014±0.00005</td><td colspan="2" align="center" rowspan="1">0.00022±0.00010</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> C-reactive protein</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> High-sensitivity C-reactive protein</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1"> Leptin</td><td colspan="2" align="center" rowspan="1">-0.0000002±0.0000001</td><td colspan="2" align="center" rowspan="1">NS</td><td colspan="2" align="center" rowspan="1">NS</td></tr><tr><td align="left" rowspan="1" colspan="1">Variance-covariance matrix of random variables</td><td align="center" rowspan="1" colspan="1">Constant</td><td align="center" rowspan="1" colspan="1">Days from start</td><td align="center" rowspan="1" colspan="1">Constant</td><td align="center" rowspan="1" colspan="1">Days from start</td><td align="center" rowspan="1" colspan="1">Constant</td><td align="center" rowspan="1" colspan="1">Days from start</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Level 1 (within individuals)</italic></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Constant</td><td align="center" rowspan="1" colspan="1">0.00024±0.00001</td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">0.00035±0.00003</td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">0.00039±0.00004</td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Level 2 (between individuals)</italic></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Constant</td><td align="center" rowspan="1" colspan="1">0.00875±0.00131</td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">0.02723± 0.00408</td><td align="center" rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">0.01131±0.00171</td><td align="center" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"> Days from start centered</td><td align="center" rowspan="1" colspan="1">0.000000± 0.000000</td><td align="center" rowspan="1" colspan="1">0.000000± 0.000000</td><td align="center" rowspan="1" colspan="1">0.000000±0.000002</td><td align="center" rowspan="1" colspan="1">0.000000±0.000000</td><td align="center" rowspan="1" colspan="1">-0.000002± 0.000001</td><td align="center" rowspan="1" colspan="1">0.0000000002±0.0000000009</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t004fn001"><p>Abbreviations: <italic>re</italic>, moderate-resistance-moderate-endurance; <italic>Re</italic>, high-Resistance-moderate-endurance; <italic>rE</italic>, moderate-resistance-high-Endurance.</p></fn><fn id="t004fn002"><p>Fixed effect values are presented as estimated mean coefficients ± SEE (standard error of estimate) of BMD in g.cm<sup>-2</sup>. Random effects values presented as estimated mean variance ± SEE (BMD in g.cm<sup>-2</sup>)<sup>2</sup>. Days from start was centered around 180 days. Changes in total body lean mass, total body fat mass and central fat mass (g) from study entry.</p></fn></table-wrap-foot></table-wrap><p>Once the longitudinal changes in anthropometry, biochemistry, and nutrition were accounted for, total body BMC was observed to be negatively affected by the reduction of total body lean mass, negative energy balance, and decreased leptin. Additionally, decreases in total body fat mass contributed positively to total body BMC. Also, higher vitamin D significantly and positively contributed to total body BMD. Decreases in total body lean mass and negative energy balance significantly and independently contributed to decreases in lumbar spine BMC. In contrast, vitamin D intake had a positive association with lumbar spine BMC and BMD. Calcium intake and vitamin D were observed to be independent and significant predictors of femoral neck BMC. In contrast, other nutrition factors did not significantly contribute to observed variability within the changes in femoral neck BMD once age at baseline, sex, and compliance were accounted for.</p></sec></sec><sec sec-type="conclusions" id="sec024"><title>Discussion</title><p>The major finding of the present study was that despite minimal effects of exercise modalities, compliance with an intensive exercise program resulted in a significantly higher bone mass during energy restriction than non-compliance. More specifically, when assessed cross-sectionally, the effects of compliance on BMC and BMD was only noted during sporadic time points; however to draw conclusions based on these cross sectional observations, we ignored nutrition and other potential links to bone mass such as age, sex, anthropometry and compliance. When BMC and BMD parameters were assessed longitudinally and the independent effects of size, body composition, dietary intake and physical activity were accounted and individuals were allowed to have their own regression lines with separate intercepts and slope coefficients, an independent and significant increment of about 12%, 6% and 5% was noted for lumbar spine BMC, lumbar spine BMD and femoral neck BMD, respectively, for participants compliant with the exercise prescription across the intervention, whereas no changes were observed in less compliant participants.</p><sec id="sec025"><title>Effects of intervention and training modalities</title><p>Despite resistance training previously demonstrating increases in BMD at hip and lumbar spine sites [<xref rid="pone.0136491.ref031" ref-type="bibr">31</xref>], the results of the current study did not demonstrate differences in bone parameters related to modalities of exercise. The high volume of prescribed resistance training in all groups of physical activity (4 times a week i.e. 6 hours per week in our study) may explain the absence of differences in the results. In the present study, despite a rapid and sustainable weight loss in MetS participants, the BMC and BMD did not differ from controls. Despite that the amount of weight loss observed in participants in the present study had previously been associated with decreases in BMD and BMC [<xref rid="pone.0136491.ref032" ref-type="bibr">32</xref>,<xref rid="pone.0136491.ref033" ref-type="bibr">33</xref>,<xref rid="pone.0136491.ref034" ref-type="bibr">34</xref>], our data support that energy restriction did not necessarily result in bone loss [<xref rid="pone.0136491.ref004" ref-type="bibr">4</xref>,<xref rid="pone.0136491.ref005" ref-type="bibr">5</xref>,<xref rid="pone.0136491.ref006" ref-type="bibr">6</xref>,<xref rid="pone.0136491.ref007" ref-type="bibr">7</xref>]. The dose-response effect of exercise on bone is well established [<xref rid="pone.0136491.ref035" ref-type="bibr">35</xref>] and may explain this absence of bone loss, as demonstrated in comparisons between compliant and non-compliant participants.</p></sec><sec id="sec026"><title>Effects of compliance</title><p>In our study, compliant participants increased BMC and BMD more than non-compliant participants, independent of the training groups. Thus, compliance appeared to be the most predominant factor for maximizing bone benefits [<xref rid="pone.0136491.ref022" ref-type="bibr">22</xref>]. Factors affecting compliance are numerous and should be taken into account in lifestyle interventions [<xref rid="pone.0136491.ref036" ref-type="bibr">36</xref>]. We report that all sites (total body, lumbar spine and femoral neck, for both BMC and BMD) in compliant participants benefited from the training, whereas previous literature has commonly reported site-specific improvements [<xref rid="pone.0136491.ref037" ref-type="bibr">37</xref>,<xref rid="pone.0136491.ref038" ref-type="bibr">38</xref>]. This could be due to the high level of physical activity (15–20 hours/week) imposed on individuals who were sedentary at baseline, with resistance training stimulating all parts of the body. However, it also demonstrates that our relatively high-volume training had no catabolic effect on bone metabolism, as encountered in overtraining [<xref rid="pone.0136491.ref039" ref-type="bibr">39</xref>]. Moreover, some bone parameters improved as acutely at the end of the residential program which reinforces the immediate potential benefits of physical activity [<xref rid="pone.0136491.ref007" ref-type="bibr">7</xref>,<xref rid="pone.0136491.ref019" ref-type="bibr">19</xref>,<xref rid="pone.0136491.ref020" ref-type="bibr">20</xref>]. As the resorption phase of bone modelling lasts 2–3 weeks and the formation phase 2–3 months [<xref rid="pone.0136491.ref040" ref-type="bibr">40</xref>], it would be surprising to have an increase in BMD and BMC after only three weeks of physical activity. However, such data have been previously reported on individuals with MetS [<xref rid="pone.0136491.ref041" ref-type="bibr">41</xref>]. Moreover, it has been shown that individuals with fractures may achieve a significant increase in BMC and BMD after three weeks [<xref rid="pone.0136491.ref042" ref-type="bibr">42</xref>].</p></sec><sec id="sec027"><title>Other explaining variables</title><p>In the current study, caloric restriction decreased BMC, in agreement with previous studies [<xref rid="pone.0136491.ref002" ref-type="bibr">2</xref>,<xref rid="pone.0136491.ref003" ref-type="bibr">3</xref>]. We confirmed the importance of adequate sources of calcium [<xref rid="pone.0136491.ref043" ref-type="bibr">43</xref>,<xref rid="pone.0136491.ref044" ref-type="bibr">44</xref>] and vitamin D [<xref rid="pone.0136491.ref045" ref-type="bibr">45</xref>] on BMC and BMD with a multilevel approach. Then, we demonstrated that lean body mass, fat mass and leptin contributed to the prediction of changes in bone parameters. Fat tissue is an active endocrine organ secreting a host of molecules called adipokines, such as leptin. We have demonstrated the likelihood of bone-adiposity cross-talk in a recent literature review [<xref rid="pone.0136491.ref046" ref-type="bibr">46</xref>]. We were unable to report that insulin, pro-inflammatory cytokines, adiponectin, PAI-1, nor even bone metabolism markers were predictor variables of weight loss. Even if these factors have previously been linked to BMC and BMD [<xref rid="pone.0136491.ref043" ref-type="bibr">43</xref>], other studies have not taken into account repeated measurements, variations in the correlations over time, and the time difference between measurements. Nevertheless, throughout the one-year intervention, females showed fewer BMC and BMD benefits from physical activity than males. Even if being a male has a positive effect on BMD [<xref rid="pone.0136491.ref044" ref-type="bibr">44</xref>], we report more extensive monitoring of gender differences in BMC and BMD changes throughout a long-term intervention program than previously published.</p></sec><sec id="sec028"><title>Limitations</title><p>This study has some limitations. A MetS group without any intervention or physical activity could have provided opportunities to distinguish the effects of a total intervention or physical activity from the diet. Implementing our intervention into health practices could be costly and our high volume training protocol (15–20 hours per week) may be difficult to comply with under non-supervised, community-based circumstances. However, we demonstrated the feasibility of such training volumes in a large sample size of sedentary obese individuals over 50 years old. The absence of direct bone metabolism measurements could also be seen as a limitation. Bone geometric data was not included in the current study. Eventually, despite some non-significant results, the multilevel hierarchical approach highlighted the effects of physical activity compliance on the prevention of bone loss classically associated with weight loss. However, compliance could only be judged <italic>at posteriori</italic> because a randomization based on compliance lacks predictability. Further studies should focus on psychological factors aimed at improving compliance in such long-term community-based intervention.</p></sec></sec><sec sec-type="conclusions" id="sec029"><title>Conclusion</title><p>Changes in bone parameters did not relate to the modalities of exercise. However, independent of physical activity groups, compliance with an intensive exercise program resulted in a significantly higher bone mass during energy restriction than non-compliance. Exercise is therefore beneficial to bone in the context of a weight loss program. Further studies should focus on maximising compliance in such long-term community-based interventions.</p></sec><sec sec-type="supplementary-material" id="sec030"><title>Supporting Information</title><supplementary-material content-type="local-data" id="pone.0136491.s001"><label>S1 CONSORT</label><caption><title>CONSORT guidelines.</title><p>(DOC)</p></caption><media xlink:href="pone.0136491.s001.doc"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="pone.0136491.s002"><label>S1 Database</label><caption><title>Titles of columns are written without abbreviations.</title><p>999 = missing data.</p><p>(XLSX)</p></caption><media xlink:href="pone.0136491.s002.xlsx"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="pone.0136491.s003"><label>S1 Protocol</label><caption><title>Study protocol.</title><p>(DOCX)</p></caption><media xlink:href="pone.0136491.s003.docx"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material></sec></body><back><ack><p>We express our sincere gratitude to the thermal baths of Chatel-Guyon and the Omental Thermalia, Chatel-Guyon, which contributed to the study.</p></ack><ref-list><title>References</title><ref id="pone.0136491.ref001"><label>1</label><mixed-citation publication-type="journal"><name><surname>Pedersen</surname><given-names>SD</given-names></name> (<year>2013</year>) <article-title>Metabolic complications of obesity</article-title>. <source>Best Pract Res Clin Endocrinol Metab</source><volume>27</volume>: <fpage>179</fpage>–<lpage>193</lpage>. <comment>doi: <ext-link ext-link-type="uri" xlink:href="http://dx.doi.org/10.1016/j.beem.2013.02.004">10.1016/j.beem.2013.02.004</ext-link></comment><pub-id pub-id-type="pmid">23731880</pub-id></mixed-citation></ref><ref 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