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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting</title><author><name sortKey="Biesemann, Christoph" sort="Biesemann, Christoph" uniqKey="Biesemann C" first="Christoph" last="Biesemann">Christoph Biesemann</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Gr Nborg, Mads" sort="Gr Nborg, Mads" uniqKey="Gr Nborg M" first="Mads" last="Gr Nborg">Mads Gr Nborg</name><affiliation><nlm:aff id="au2"><institution>Department of Neurobiology, Max Planck Institute of Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au10"><institution>Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Luquet, Elisa" sort="Luquet, Elisa" uniqKey="Luquet E" first="Elisa" last="Luquet">Elisa Luquet</name><affiliation><nlm:aff id="au3"><institution>CNRS, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au4"><institution>University of Bordeaux, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wichert, Sven P" sort="Wichert, Sven P" uniqKey="Wichert S" first="Sven P" last="Wichert">Sven P. Wichert</name><affiliation><nlm:aff id="au5"><institution>Gene Expression Group, Department of Neurogenetics, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bernard, Veronique" sort="Bernard, Veronique" uniqKey="Bernard V" first="Véronique" last="Bernard">Véronique Bernard</name><affiliation><nlm:aff id="au6"><institution>INSERM U952, Université Pierre et Marie Curie</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au7"><institution>CNRS UMR 7224</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au8"><institution>Université Pierre et Marie Curie (UPMC) Paris 06, PMSNC</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bungers, Simon R" sort="Bungers, Simon R" uniqKey="Bungers S" first="Simon R" last="Bungers">Simon R. Bungers</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cooper, Ben" sort="Cooper, Ben" uniqKey="Cooper B" first="Ben" last="Cooper">Ben Cooper</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Varoqueaux, Frederique" sort="Varoqueaux, Frederique" uniqKey="Varoqueaux F" first="Frédérique" last="Varoqueaux">Frédérique Varoqueaux</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Liyi" sort="Li, Liyi" uniqKey="Li L" first="Liyi" last="Li">Liyi Li</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Byrne, Jennifer A" sort="Byrne, Jennifer A" uniqKey="Byrne J" first="Jennifer A" last="Byrne">Jennifer A. Byrne</name><affiliation><nlm:aff id="au9"><institution>Molecular Oncology Laboratory, Children's Cancer Research Unit, Kids Research Institute</institution><addr-line>Westmead, NSW, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Urlaub, Henning" sort="Urlaub, Henning" uniqKey="Urlaub H" first="Henning" last="Urlaub">Henning Urlaub</name><affiliation><nlm:aff id="au10"><institution>Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au11"><institution>Bioanalytics, Department of Clinical Chemistry, University Medical Center Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Jahn, Olaf" sort="Jahn, Olaf" uniqKey="Jahn O" first="Olaf" last="Jahn">Olaf Jahn</name><affiliation><nlm:aff id="au12"><institution>Proteomics Group, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Brose, Nils" sort="Brose, Nils" uniqKey="Brose N" first="Nils" last="Brose">Nils Brose</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Herzog, Etienne" sort="Herzog, Etienne" uniqKey="Herzog E" first="Etienne" last="Herzog">Etienne Herzog</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au3"><institution>CNRS, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au4"><institution>University of Bordeaux, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au6"><institution>INSERM U952, Université Pierre et Marie Curie</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au7"><institution>CNRS UMR 7224</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au8"><institution>Université Pierre et Marie Curie (UPMC) Paris 06, PMSNC</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24413018</idno><idno type="pmc">3989609</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989609</idno><idno type="RBID">PMC:3989609</idno><idno type="doi">10.1002/embj.201386120</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000184</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000184</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting</title><author><name sortKey="Biesemann, Christoph" sort="Biesemann, Christoph" uniqKey="Biesemann C" first="Christoph" last="Biesemann">Christoph Biesemann</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Gr Nborg, Mads" sort="Gr Nborg, Mads" uniqKey="Gr Nborg M" first="Mads" last="Gr Nborg">Mads Gr Nborg</name><affiliation><nlm:aff id="au2"><institution>Department of Neurobiology, Max Planck Institute of Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au10"><institution>Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Luquet, Elisa" sort="Luquet, Elisa" uniqKey="Luquet E" first="Elisa" last="Luquet">Elisa Luquet</name><affiliation><nlm:aff id="au3"><institution>CNRS, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au4"><institution>University of Bordeaux, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wichert, Sven P" sort="Wichert, Sven P" uniqKey="Wichert S" first="Sven P" last="Wichert">Sven P. Wichert</name><affiliation><nlm:aff id="au5"><institution>Gene Expression Group, Department of Neurogenetics, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bernard, Veronique" sort="Bernard, Veronique" uniqKey="Bernard V" first="Véronique" last="Bernard">Véronique Bernard</name><affiliation><nlm:aff id="au6"><institution>INSERM U952, Université Pierre et Marie Curie</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au7"><institution>CNRS UMR 7224</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au8"><institution>Université Pierre et Marie Curie (UPMC) Paris 06, PMSNC</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bungers, Simon R" sort="Bungers, Simon R" uniqKey="Bungers S" first="Simon R" last="Bungers">Simon R. Bungers</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cooper, Ben" sort="Cooper, Ben" uniqKey="Cooper B" first="Ben" last="Cooper">Ben Cooper</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Varoqueaux, Frederique" sort="Varoqueaux, Frederique" uniqKey="Varoqueaux F" first="Frédérique" last="Varoqueaux">Frédérique Varoqueaux</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Liyi" sort="Li, Liyi" uniqKey="Li L" first="Liyi" last="Li">Liyi Li</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Byrne, Jennifer A" sort="Byrne, Jennifer A" uniqKey="Byrne J" first="Jennifer A" last="Byrne">Jennifer A. Byrne</name><affiliation><nlm:aff id="au9"><institution>Molecular Oncology Laboratory, Children's Cancer Research Unit, Kids Research Institute</institution><addr-line>Westmead, NSW, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Urlaub, Henning" sort="Urlaub, Henning" uniqKey="Urlaub H" first="Henning" last="Urlaub">Henning Urlaub</name><affiliation><nlm:aff id="au10"><institution>Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au11"><institution>Bioanalytics, Department of Clinical Chemistry, University Medical Center Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Jahn, Olaf" sort="Jahn, Olaf" uniqKey="Jahn O" first="Olaf" last="Jahn">Olaf Jahn</name><affiliation><nlm:aff id="au12"><institution>Proteomics Group, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Brose, Nils" sort="Brose, Nils" uniqKey="Brose N" first="Nils" last="Brose">Nils Brose</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Herzog, Etienne" sort="Herzog, Etienne" uniqKey="Herzog E" first="Etienne" last="Herzog">Etienne Herzog</name><affiliation><nlm:aff id="au1"><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au3"><institution>CNRS, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au4"><institution>University of Bordeaux, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au6"><institution>INSERM U952, Université Pierre et Marie Curie</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au7"><institution>CNRS UMR 7224</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au8"><institution>Université Pierre et Marie Curie (UPMC) Paris 06, PMSNC</institution><addr-line>Paris, France</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">The EMBO Journal</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>For decades, neuroscientists have used enriched preparations of synaptic particles called synaptosomes to study synapse function. However, the interpretation of corresponding data is problematic as synaptosome preparations contain multiple types of synapses and non-synaptic neuronal and glial contaminants. We established a novel Fluorescence Activated Synaptosome Sorting (FASS) method that substantially improves conventional synaptosome enrichment protocols and enables high-resolution biochemical analyses of specific synapse subpopulations. Employing knock-in mice with fluorescent glutamatergic synapses, we show that FASS isolates intact ultrapure synaptosomes composed of a resealed presynaptic terminal and a postsynaptic density as assessed by light and electron microscopy. FASS synaptosomes contain <italic>bona fide</italic> glutamatergic synapse proteins but are almost devoid of other synapse types and extrasynaptic or glial contaminants. We identified 163 enriched proteins in FASS samples, of which FXYD6 and Tpd52 were validated as new synaptic proteins. FASS purification thus enables high-resolution biochemical analyses of specific synapse subpopulations in health and disease.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">EMBO J</journal-id><journal-id journal-id-type="iso-abbrev">EMBO J</journal-id><journal-id journal-id-type="publisher-id">embj</journal-id><journal-title-group><journal-title>The EMBO Journal</journal-title></journal-title-group><issn pub-type="ppub">0261-4189</issn><issn pub-type="epub">1460-2075</issn><publisher><publisher-name>Blackwell Publishing Ltd</publisher-name><publisher-loc>Oxford, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24413018</article-id><article-id pub-id-type="pmc">3989609</article-id><article-id pub-id-type="doi">10.1002/embj.201386120</article-id><article-categories><subj-group subj-group-type="heading"><subject>Resource</subject></subj-group></article-categories><title-group><article-title>Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Biesemann</surname><given-names>Christoph</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Grønborg</surname><given-names>Mads</given-names></name><xref ref-type="aff" rid="au2">2</xref><xref ref-type="aff" rid="au10">10</xref><xref ref-type="author-notes" rid="fn1">†</xref></contrib><contrib contrib-type="author"><name><surname>Luquet</surname><given-names>Elisa</given-names></name><xref ref-type="aff" rid="au3">3</xref><xref ref-type="aff" rid="au4">4</xref></contrib><contrib contrib-type="author"><name><surname>Wichert</surname><given-names>Sven P</given-names></name><xref ref-type="aff" rid="au5">5</xref><xref ref-type="author-notes" rid="fn2">‡</xref></contrib><contrib contrib-type="author"><name><surname>Bernard</surname><given-names>Véronique</given-names></name><xref ref-type="aff" rid="au6">6</xref><xref ref-type="aff" rid="au7">7</xref><xref ref-type="aff" rid="au8">8</xref></contrib><contrib contrib-type="author"><name><surname>Bungers</surname><given-names>Simon R</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Cooper</surname><given-names>Ben</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Varoqueaux</surname><given-names>Frédérique</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Liyi</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Byrne</surname><given-names>Jennifer A</given-names></name><xref ref-type="aff" rid="au9">9</xref></contrib><contrib contrib-type="author"><name><surname>Urlaub</surname><given-names>Henning</given-names></name><xref ref-type="aff" rid="au10">10</xref><xref ref-type="aff" rid="au11">11</xref></contrib><contrib contrib-type="author"><name><surname>Jahn</surname><given-names>Olaf</given-names></name><xref ref-type="aff" rid="au12">12</xref></contrib><contrib contrib-type="author"><name><surname>Brose</surname><given-names>Nils</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Herzog</surname><given-names>Etienne</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="aff" rid="au3">3</xref><xref ref-type="aff" rid="au4">4</xref><xref ref-type="aff" rid="au6">6</xref><xref ref-type="aff" rid="au7">7</xref><xref ref-type="aff" rid="au8">8</xref><xref ref-type="corresp" rid="cor2">**</xref></contrib><aff id="au1"><label>1</label><institution>Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></aff><aff id="au2"><label>2</label><institution>Department of Neurobiology, Max Planck Institute of Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></aff><aff id="au3"><label>3</label><institution>CNRS, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></aff><aff id="au4"><label>4</label><institution>University of Bordeaux, IINS, UMR 5297</institution><addr-line>Bordeaux, France</addr-line></aff><aff id="au5"><label>5</label><institution>Gene Expression Group, Department of Neurogenetics, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></aff><aff id="au6"><label>6</label><institution>INSERM U952, Université Pierre et Marie Curie</institution><addr-line>Paris, France</addr-line></aff><aff id="au7"><label>7</label><institution>CNRS UMR 7224</institution><addr-line>Paris, France</addr-line></aff><aff id="au8"><label>8</label><institution>Université Pierre et Marie Curie (UPMC) Paris 06, PMSNC</institution><addr-line>Paris, France</addr-line></aff><aff id="au9"><label>9</label><institution>Molecular Oncology Laboratory, Children's Cancer Research Unit, Kids Research Institute</institution><addr-line>Westmead, NSW, Australia</addr-line></aff><aff id="au10"><label>10</label><institution>Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry</institution><addr-line>Göttingen, Germany</addr-line></aff><aff id="au11"><label>11</label><institution>Bioanalytics, Department of Clinical Chemistry, University Medical Center Göttingen</institution><addr-line>Göttingen, Germany</addr-line></aff><aff id="au12"><label>12</label><institution>Proteomics Group, Max Planck Institute of Experimental Medicine</institution><addr-line>Göttingen, Germany</addr-line></aff></contrib-group><author-notes><corresp id="cor1">*Corresponding author. Tel: +49 551 3899 725; Fax: +49 551 3899 715; E-mail: <email>brose@em.mpg.de</email></corresp><corresp id="cor2">**Corresponding author. Tel: +33 5 57 57 57 48; Fax: +33 5 57 57 40 82; E-mail: <email>etienne.herzog@u-bordeaux2.fr</email></corresp><fn id="fn1"><label>†</label><p>Department of Beta Cell Regeneration, Hagedorn Research Institute, Gentofte, Denmark</p></fn><fn id="fn2"><label>‡</label><p>Department of Psychiatry and Psychotherapy, Ludwig-Maximilians-University Munich, Munich, Germany</p></fn><fn><p><bold>Subject Categories</bold> Neuroscience; Methods & Resources</p></fn></author-notes><pub-date pub-type="ppub"><day>16</day><month>1</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>10</day><month>1</month><year>2014</year></pub-date><volume>33</volume><issue>2</issue><fpage>157</fpage><lpage>170</lpage><history><date date-type="received"><day>28</day><month>6</month><year>2013</year></date><date date-type="rev-recd"><day>10</day><month>11</month><year>2013</year></date><date date-type="accepted"><day>18</day><month>11</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 The Authors</copyright-statement><copyright-year>2013</copyright-year></permissions><abstract><p>For decades, neuroscientists have used enriched preparations of synaptic particles called synaptosomes to study synapse function. However, the interpretation of corresponding data is problematic as synaptosome preparations contain multiple types of synapses and non-synaptic neuronal and glial contaminants. We established a novel Fluorescence Activated Synaptosome Sorting (FASS) method that substantially improves conventional synaptosome enrichment protocols and enables high-resolution biochemical analyses of specific synapse subpopulations. Employing knock-in mice with fluorescent glutamatergic synapses, we show that FASS isolates intact ultrapure synaptosomes composed of a resealed presynaptic terminal and a postsynaptic density as assessed by light and electron microscopy. FASS synaptosomes contain <italic>bona fide</italic> glutamatergic synapse proteins but are almost devoid of other synapse types and extrasynaptic or glial contaminants. We identified 163 enriched proteins in FASS samples, of which FXYD6 and Tpd52 were validated as new synaptic proteins. FASS purification thus enables high-resolution biochemical analyses of specific synapse subpopulations in health and disease.</p></abstract><kwd-group><kwd>Fluorescence Activated Synaptosome Sorting</kwd><kwd>proteomics</kwd><kwd>subcellular fractionation</kwd><kwd>synaptosome</kwd><kwd>vesicular glutamate transporter</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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