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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential use of autophagy by primary dendritic cells specialized in cross-presentation</title><author><name sortKey="Mintern, Justine D" sort="Mintern, Justine D" uniqKey="Mintern J" first="Justine D" last="Mintern">Justine D. Mintern</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Macri, Christophe" sort="Macri, Christophe" uniqKey="Macri C" first="Christophe" last="Macri">Christophe Macri</name><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Chin, Wei Jin" sort="Chin, Wei Jin" uniqKey="Chin W" first="Wei Jin" last="Chin">Wei Jin Chin</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Panozza, Scott E" sort="Panozza, Scott E" uniqKey="Panozza S" first="Scott E" last="Panozza">Scott E. Panozza</name><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Segura, Elodie" sort="Segura, Elodie" uniqKey="Segura E" first="Elodie" last="Segura">Elodie Segura</name><affiliation><nlm:aff id="af0004"><institution>Institut National de la Santé et de la Recherche Medicale Unite 932</institution>; Paris,<country>France</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0005"><institution>Centre de Recherche; Institut Curie</institution>; Paris,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Patterson, Natalie L" sort="Patterson, Natalie L" uniqKey="Patterson N" first="Natalie L" last="Patterson">Natalie L. Patterson</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Zeller, Peter" sort="Zeller, Peter" uniqKey="Zeller P" first="Peter" last="Zeller">Peter Zeller</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Bourges, Dorothee" sort="Bourges, Dorothee" uniqKey="Bourges D" first="Dorothee" last="Bourges">Dorothee Bourges</name><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Bedoui, Sammy" sort="Bedoui, Sammy" uniqKey="Bedoui S" first="Sammy" last="Bedoui">Sammy Bedoui</name><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Mcmillan, Paul J" sort="Mcmillan, Paul J" uniqKey="Mcmillan P" first="Paul J" last="Mcmillan">Paul J. Mcmillan</name><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0006"><institution>Biological Optical Microscopy Platform; University of Melbourne</institution>, Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0007"><institution>ARC Center of Excellence for Coherent X-ray Science; University of Melbourne</institution>, Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Idris, Adi" sort="Idris, Adi" uniqKey="Idris A" first="Adi" last="Idris">Adi Idris</name><affiliation><nlm:aff id="af0008"><institution>Mater Research Institute, University of Queensland; Translational Research Institute</institution>, Woolloongabba, QLD<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Nowell, Cameron J" sort="Nowell, Cameron J" uniqKey="Nowell C" first="Cameron J" last="Nowell">Cameron J. Nowell</name><affiliation><nlm:aff id="af0009"><institution>Center for Advanced Microscopy; Ludwig Institute for Cancer Research</institution>; Melbourne, Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0010"><institution>Monash Institute of Pharmaceutical Sciences</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Brown, Andrew" sort="Brown, Andrew" uniqKey="Brown A" first="Andrew" last="Brown">Andrew Brown</name><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Radford, Kristen J" sort="Radford, Kristen J" uniqKey="Radford K" first="Kristen J" last="Radford">Kristen J. Radford</name><affiliation><nlm:aff id="af0008"><institution>Mater Research Institute, University of Queensland; Translational Research Institute</institution>, Woolloongabba, QLD<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Johnston, Angus Pr" sort="Johnston, Angus Pr" uniqKey="Johnston A" first="Angus Pr" last="Johnston">Angus Pr Johnston</name><affiliation><nlm:aff id="af0010"><institution>Monash Institute of Pharmaceutical Sciences</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Villadangos, Jose A" sort="Villadangos, Jose A" uniqKey="Villadangos J" first="Jose A" last="Villadangos">Jose A. Villadangos</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25950899</idno><idno type="pmc">4502655</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502655</idno><idno type="RBID">PMC:4502655</idno><idno type="doi">10.1080/15548627.2015.1045178</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000170</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000170</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Differential use of autophagy by primary dendritic cells specialized in cross-presentation</title><author><name sortKey="Mintern, Justine D" sort="Mintern, Justine D" uniqKey="Mintern J" first="Justine D" last="Mintern">Justine D. Mintern</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Macri, Christophe" sort="Macri, Christophe" uniqKey="Macri C" first="Christophe" last="Macri">Christophe Macri</name><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Chin, Wei Jin" sort="Chin, Wei Jin" uniqKey="Chin W" first="Wei Jin" last="Chin">Wei Jin Chin</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Panozza, Scott E" sort="Panozza, Scott E" uniqKey="Panozza S" first="Scott E" last="Panozza">Scott E. Panozza</name><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Segura, Elodie" sort="Segura, Elodie" uniqKey="Segura E" first="Elodie" last="Segura">Elodie Segura</name><affiliation><nlm:aff id="af0004"><institution>Institut National de la Santé et de la Recherche Medicale Unite 932</institution>; Paris,<country>France</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0005"><institution>Centre de Recherche; Institut Curie</institution>; Paris,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Patterson, Natalie L" sort="Patterson, Natalie L" uniqKey="Patterson N" first="Natalie L" last="Patterson">Natalie L. Patterson</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Zeller, Peter" sort="Zeller, Peter" uniqKey="Zeller P" first="Peter" last="Zeller">Peter Zeller</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Bourges, Dorothee" sort="Bourges, Dorothee" uniqKey="Bourges D" first="Dorothee" last="Bourges">Dorothee Bourges</name><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Bedoui, Sammy" sort="Bedoui, Sammy" uniqKey="Bedoui S" first="Sammy" last="Bedoui">Sammy Bedoui</name><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Mcmillan, Paul J" sort="Mcmillan, Paul J" uniqKey="Mcmillan P" first="Paul J" last="Mcmillan">Paul J. Mcmillan</name><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0006"><institution>Biological Optical Microscopy Platform; University of Melbourne</institution>, Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0007"><institution>ARC Center of Excellence for Coherent X-ray Science; University of Melbourne</institution>, Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Idris, Adi" sort="Idris, Adi" uniqKey="Idris A" first="Adi" last="Idris">Adi Idris</name><affiliation><nlm:aff id="af0008"><institution>Mater Research Institute, University of Queensland; Translational Research Institute</institution>, Woolloongabba, QLD<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Nowell, Cameron J" sort="Nowell, Cameron J" uniqKey="Nowell C" first="Cameron J" last="Nowell">Cameron J. Nowell</name><affiliation><nlm:aff id="af0009"><institution>Center for Advanced Microscopy; Ludwig Institute for Cancer Research</institution>; Melbourne, Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0010"><institution>Monash Institute of Pharmaceutical Sciences</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Brown, Andrew" sort="Brown, Andrew" uniqKey="Brown A" first="Andrew" last="Brown">Andrew Brown</name><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Radford, Kristen J" sort="Radford, Kristen J" uniqKey="Radford K" first="Kristen J" last="Radford">Kristen J. Radford</name><affiliation><nlm:aff id="af0008"><institution>Mater Research Institute, University of Queensland; Translational Research Institute</institution>, Woolloongabba, QLD<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Johnston, Angus Pr" sort="Johnston, Angus Pr" uniqKey="Johnston A" first="Angus Pr" last="Johnston">Angus Pr Johnston</name><affiliation><nlm:aff id="af0010"><institution>Monash Institute of Pharmaceutical Sciences</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Villadangos, Jose A" sort="Villadangos, Jose A" uniqKey="Villadangos J" first="Jose A" last="Villadangos">Jose A. Villadangos</name><affiliation><nlm:aff id="af0001"><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0002"><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation><affiliation><nlm:aff id="af0003"><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></nlm:aff></affiliation></author></analytic><series><title level="j">Autophagy</title><idno type="ISSN">1554-8627</idno><idno type="eISSN">1554-8635</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Antigen-presenting cells survey their environment and present captured antigens bound to major histocompatibility complex (MHC) molecules. Formation of MHC-antigen complexes occurs in specialized compartments where multiple protein trafficking routes, still incompletely understood, converge. Autophagy is a route that enables the presentation of cytosolic antigen by MHC class II molecules. Some reports also implicate autophagy in the presentation of extracellular, endocytosed antigen by MHC class I molecules, a pathway termed “cross-presentation.” The role of autophagy in cross-presentation is controversial. This may be due to studies using different types of antigen presenting cells for which the use of autophagy is not well defined. Here we report that active use of autophagy is evident only in DC subtypes specialized in cross-presentation. However, the contribution of autophagy to cross-presentation varied depending on the form of antigen: it was negligible in the case of cell-associated antigen or antigen delivered via receptor-mediated endocytosis, but more prominent when the antigen was a soluble protein. These findings highlight the differential use of autophagy and its machinery by primary cells equipped with specific immune function, and prompt careful reassessment of the participation of this endocytic pathway in antigen cross-presentation.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Autophagy</journal-id><journal-id journal-id-type="iso-abbrev">Autophagy</journal-id><journal-id journal-id-type="pmc">KAUP</journal-id><journal-title-group><journal-title>Autophagy</journal-title></journal-title-group><issn pub-type="ppub">1554-8627</issn><issn pub-type="epub">1554-8635</issn><publisher><publisher-name>Taylor & Francis</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25950899</article-id><article-id pub-id-type="pmc">4502655</article-id><article-id pub-id-type="publisher-id">1045178</article-id><article-id pub-id-type="doi">10.1080/15548627.2015.1045178</article-id><article-categories><subj-group subj-group-type="heading"><subject>Basic Research Papers</subject></subj-group></article-categories><title-group><article-title>Differential use of autophagy by primary dendritic cells specialized in cross-presentation</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mintern</surname><given-names>Justine D</given-names></name><xref ref-type="aff" rid="af0001"><sup>1</sup></xref><xref ref-type="aff" rid="af0002"><sup>2</sup></xref><xref ref-type="corresp" rid="an0001"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Macri</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="af0002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chin</surname><given-names>Wei Jin</given-names></name><xref ref-type="aff" rid="af0001"><sup>1</sup></xref><xref ref-type="aff" rid="af0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Panozza</surname><given-names>Scott E</given-names></name><xref ref-type="aff" rid="af0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Segura</surname><given-names>Elodie</given-names></name><xref ref-type="aff" rid="af0004"><sup>4</sup></xref><xref ref-type="aff" rid="af0005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Patterson</surname><given-names>Natalie L</given-names></name><xref ref-type="aff" rid="af0001"><sup>1</sup></xref><xref ref-type="aff" rid="af0002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zeller</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="af0001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bourges</surname><given-names>Dorothee</given-names></name><xref ref-type="aff" rid="af0002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bedoui</surname><given-names>Sammy</given-names></name><xref ref-type="aff" rid="af0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>McMillan</surname><given-names>Paul J</given-names></name><xref ref-type="aff" rid="af0002"><sup>2</sup></xref><xref ref-type="aff" rid="af0006"><sup>6</sup></xref><xref ref-type="aff" rid="af0007"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Idris</surname><given-names>Adi</given-names></name><xref ref-type="aff" rid="af0008"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nowell</surname><given-names>Cameron J</given-names></name><xref ref-type="aff" rid="af0009"><sup>9</sup></xref><xref ref-type="aff" rid="af0010"><sup>10</sup></xref></contrib><contrib contrib-type="author"><name><surname>Brown</surname><given-names>Andrew</given-names></name><xref ref-type="aff" rid="af0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Radford</surname><given-names>Kristen J</given-names></name><xref ref-type="aff" rid="af0008"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Johnston</surname><given-names>Angus PR</given-names></name><xref ref-type="aff" rid="af0010"><sup>10</sup></xref></contrib><contrib contrib-type="author"><name><surname>Villadangos</surname><given-names>Jose A</given-names></name><xref ref-type="aff" rid="af0001"><sup>1</sup></xref><xref ref-type="aff" rid="af0002"><sup>2</sup></xref><xref ref-type="aff" rid="af0003"><sup>3</sup></xref><xref ref-type="corresp" rid="an0001"><sup>*</sup></xref></contrib><aff id="af0001"><label>1</label><institution>Walter and Eliza Hall Institute</institution>; Parkville, VIC<country>Australia</country></aff><aff id="af0002"><label>2</label><institution>Department of Biochemistry and Molecular Biology; University of Melbourne; Bio21 Molecular Science and Biotechnology Institute</institution>; Parkville, VIC<country>Australia</country></aff><aff id="af0003"><label>3</label><institution>Department of Microbiology and Immunology; Peter Doherty Institute for Infection and Immunity; University of Melbourne</institution>; Parkville, VIC<country>Australia</country></aff><aff id="af0004"><label>4</label><institution>Institut National de la Santé et de la Recherche Medicale Unite 932</institution>; Paris,<country>France</country></aff><aff id="af0005"><label>5</label><institution>Centre de Recherche; Institut Curie</institution>; Paris,<country>France</country></aff><aff id="af0006"><label>6</label><institution>Biological Optical Microscopy Platform; University of Melbourne</institution>, Parkville, VIC<country>Australia</country></aff><aff id="af0007"><label>7</label><institution>ARC Center of Excellence for Coherent X-ray Science; University of Melbourne</institution>, Parkville, VIC<country>Australia</country></aff><aff id="af0008"><label>8</label><institution>Mater Research Institute, University of Queensland; Translational Research Institute</institution>, Woolloongabba, QLD<country>Australia</country></aff><aff id="af0009"><label>9</label><institution>Center for Advanced Microscopy; Ludwig Institute for Cancer Research</institution>; Melbourne, Parkville, VIC<country>Australia</country></aff><aff id="af0010"><label>10</label><institution>Monash Institute of Pharmaceutical Sciences</institution>; Parkville, VIC<country>Australia</country></aff></contrib-group><author-notes><corresp id="an0001"><label>*</label>Correspondence to: Justine D Mintern; Email: <email xlink:href="jmintern@unimelb.edu.au">jmintern@unimelb.edu.au</email>, Jose A. Villadangos; Email: <email xlink:href="j.villadangos@unimelb.edu.au">j.villadangos@unimelb.edu.au</email></corresp></author-notes><pub-date pub-type="epub"><day>7</day><month>5</month><year>2015</year></pub-date><pub-date pub-type="collection"><month>6</month><year>2015</year></pub-date><volume>11</volume><issue>6</issue><fpage seq="5">906</fpage><lpage>917</lpage><history><date date-type="received"><day>31</day><month>3</month><year>2014</year></date><date date-type="rev-recd"><day>22</day><month>4</month><year>2015</year></date><date date-type="accepted"><day>22</day><month>4</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 Taylor & Francis Group, LLC</copyright-statement><copyright-year>2015</copyright-year><copyright-holder>Taylor & Francis Group, LLC</copyright-holder></permissions><self-uri content-type="pdf" xlink:href="kaup-11-06-1045178.pdf"></self-uri><abstract><p>Antigen-presenting cells survey their environment and present captured antigens bound to major histocompatibility complex (MHC) molecules. Formation of MHC-antigen complexes occurs in specialized compartments where multiple protein trafficking routes, still incompletely understood, converge. Autophagy is a route that enables the presentation of cytosolic antigen by MHC class II molecules. Some reports also implicate autophagy in the presentation of extracellular, endocytosed antigen by MHC class I molecules, a pathway termed “cross-presentation.” The role of autophagy in cross-presentation is controversial. This may be due to studies using different types of antigen presenting cells for which the use of autophagy is not well defined. Here we report that active use of autophagy is evident only in DC subtypes specialized in cross-presentation. However, the contribution of autophagy to cross-presentation varied depending on the form of antigen: it was negligible in the case of cell-associated antigen or antigen delivered via receptor-mediated endocytosis, but more prominent when the antigen was a soluble protein. These findings highlight the differential use of autophagy and its machinery by primary cells equipped with specific immune function, and prompt careful reassessment of the participation of this endocytic pathway in antigen cross-presentation.</p></abstract><kwd-group kwd-group-type="author"><title>Keywords</title><kwd>antigen presentation</kwd><kwd>autophagy</kwd><kwd>dendritic cells</kwd></kwd-group><kwd-group kwd-group-type="author"><title>Abbreviations</title><kwd>3-MA, 3-methyladenine</kwd><kwd>Atg7-DC CKO, <italic>Atg7</italic> DC conditional knockout</kwd><kwd>BafA, bafilomycin A<sub>1</sub></kwd><kwd>CD, cluster of differentiation</kwd><kwd>CTL, cytotoxic T lymphocyte</kwd><kwd>DC, dendritic cell</kwd><kwd>DALIS, dendritic cell aggresome-like inducible structures</kwd><kwd>green fluorescent protein, GFP</kwd><kwd>IFC imaging flow cytometry</kwd><kwd>LAP, LC3 associated phagocytosis</kwd><kwd>LC3B, microtubule-associated protein 1 light chain 3 β</kwd><kwd>MHC II, major histocompatibility complex class II</kwd><kwd>MHC I, major histocompatibility complex class I</kwd><kwd>OVA, ovalbumin</kwd><kwd>OT-I, OVA-specific CD8<sup>+</sup> T cell</kwd><kwd>OT-II, OVA-specific CD4<sup>+</sup> T cell; SIM, structured illumination microscopy.</kwd></kwd-group><counts><fig-count count="4"></fig-count><table-count count="0"></table-count><ref-count count="48"></ref-count><page-count count="12"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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