Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase
Identifieur interne : 002965 ( Pmc/Checkpoint ); précédent : 002964; suivant : 002966Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase
Auteurs : Diana Stojanovski ; Dusanka Milenkovic ; Judith M. Müller ; Kipros Gabriel ; Agnes Schulze-Specking ; Michael J. Baker [Australie] ; Michael T. Ryan [Australie] ; Bernard Guiard [France] ; Nikolaus Pfanner [Allemagne] ; Agnieszka Chacinska [Allemagne]Source :
- The Journal of Cell Biology [ 0021-9525 ] ; 2008.
Abstract
The biogenesis of mitochondrial intermembrane space proteins depends on specific machinery that transfers disulfide bonds to precursor proteins. The machinery shares features with protein relays for disulfide bond formation in the bacterial periplasm and endoplasmic reticulum. A disulfide-generating enzyme/sulfhydryl oxidase oxidizes a disulfide carrier protein, which in turn transfers a disulfide to the substrate protein. Current views suggest that the disulfide carrier alternates between binding to the oxidase and the substrate. We have analyzed the cooperation of the disulfide relay components during import of precursors into mitochondria and identified a ternary complex of all three components. The ternary complex represents a transient and intermediate step in the oxidation of intermembrane space precursors, where the oxidase Erv1 promotes disulfide transfer to the precursor while both oxidase and precursor are associated with the disulfide carrier Mia40.
Url:
DOI: 10.1083/jcb.200804095
PubMed: 18852299
PubMed Central: 2568017
Affiliations:
- Allemagne, Australie, France
- Bade-Wurtemberg, District de Fribourg-en-Brisgau, Victoria (État)
- Fribourg-en-Brisgau, Melbourne
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Baker</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry, La Trobe University, Melbourne 3086, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry, La Trobe University, Melbourne 3086</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Ryan, Michael T" sort="Ryan, Michael T" uniqKey="Ryan M" first="Michael T." last="Ryan">Michael T. Ryan</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry, La Trobe University, Melbourne 3086, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry, La Trobe University, Melbourne 3086</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Guiard, Bernard" sort="Guiard, Bernard" uniqKey="Guiard B" first="Bernard" last="Guiard">Bernard Guiard</name><affiliation wicri:level="1"><nlm:aff id="aff4">Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette</wicri:regionArea><wicri:noRegion>91190 Gif-sur-Yvette</wicri:noRegion><wicri:noRegion>91190 Gif-sur-Yvette</wicri:noRegion></affiliation></author><author><name sortKey="Pfanner, Nikolaus" sort="Pfanner, Nikolaus" uniqKey="Pfanner N" first="Nikolaus" last="Pfanner">Nikolaus Pfanner</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation><affiliation wicri:level="3"><nlm:aff id="N0x3a3fdd0N0x400fa70">Centre for Biological Signalling Studies (<italic>bioss</italic>), Universität Freiburg, 79104 Freiburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>), Universität Freiburg, 79104 Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Chacinska, Agnieszka" sort="Chacinska, Agnieszka" uniqKey="Chacinska A" first="Agnieszka" last="Chacinska">Agnieszka Chacinska</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation><affiliation wicri:level="3"><nlm:aff id="N0x3a3fdd0N0x400fa70">Centre for Biological Signalling Studies (<italic>bioss</italic>), Universität Freiburg, 79104 Freiburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>), Universität Freiburg, 79104 Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18852299</idno><idno type="pmc">2568017</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2568017</idno><idno type="RBID">PMC:2568017</idno><idno type="doi">10.1083/jcb.200804095</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">001121</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001121</idno><idno type="wicri:Area/Pmc/Curation">000F82</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000F82</idno><idno type="wicri:Area/Pmc/Checkpoint">002965</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002965</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase</title><author><name sortKey="Stojanovski, Diana" sort="Stojanovski, Diana" uniqKey="Stojanovski D" first="Diana" last="Stojanovski">Diana Stojanovski</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation></author><author><name sortKey="Milenkovic, Dusanka" sort="Milenkovic, Dusanka" uniqKey="Milenkovic D" first="Dusanka" last="Milenkovic">Dusanka Milenkovic</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation></author><author><name sortKey="Muller, Judith M" sort="Muller, Judith M" uniqKey="Muller J" first="Judith M." last="Müller">Judith M. Müller</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation></author><author><name sortKey="Gabriel, Kipros" sort="Gabriel, Kipros" uniqKey="Gabriel K" first="Kipros" last="Gabriel">Kipros Gabriel</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation></author><author><name sortKey="Schulze Specking, Agnes" sort="Schulze Specking, Agnes" uniqKey="Schulze Specking A" first="Agnes" last="Schulze-Specking">Agnes Schulze-Specking</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation></author><author><name sortKey="Baker, Michael J" sort="Baker, Michael J" uniqKey="Baker M" first="Michael J." last="Baker">Michael J. Baker</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry, La Trobe University, Melbourne 3086, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry, La Trobe University, Melbourne 3086</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Ryan, Michael T" sort="Ryan, Michael T" uniqKey="Ryan M" first="Michael T." last="Ryan">Michael T. Ryan</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry, La Trobe University, Melbourne 3086, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry, La Trobe University, Melbourne 3086</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Guiard, Bernard" sort="Guiard, Bernard" uniqKey="Guiard B" first="Bernard" last="Guiard">Bernard Guiard</name><affiliation wicri:level="1"><nlm:aff id="aff4">Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette</wicri:regionArea><wicri:noRegion>91190 Gif-sur-Yvette</wicri:noRegion><wicri:noRegion>91190 Gif-sur-Yvette</wicri:noRegion></affiliation></author><author><name sortKey="Pfanner, Nikolaus" sort="Pfanner, Nikolaus" uniqKey="Pfanner N" first="Nikolaus" last="Pfanner">Nikolaus Pfanner</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation><affiliation wicri:level="3"><nlm:aff id="N0x3a3fdd0N0x400fa70">Centre for Biological Signalling Studies (<italic>bioss</italic>), Universität Freiburg, 79104 Freiburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>), Universität Freiburg, 79104 Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Chacinska, Agnieszka" sort="Chacinska, Agnieszka" uniqKey="Chacinska A" first="Agnieszka" last="Chacinska">Agnieszka Chacinska</name><affiliation><nlm:aff wicri:cut=", and" id="N0x3a3fdd0N0x400fa70">Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung</nlm:aff><wicri:noCountry code="subfield">Zentrum für Biochemie und Molekulare Zellforschung</wicri:noCountry></affiliation><affiliation wicri:level="3"><nlm:aff id="N0x3a3fdd0N0x400fa70">Centre for Biological Signalling Studies (<italic>bioss</italic>), Universität Freiburg, 79104 Freiburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>), Universität Freiburg, 79104 Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author></analytic><series><title level="j">The Journal of Cell Biology</title><idno type="ISSN">0021-9525</idno><idno type="eISSN">1540-8140</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The biogenesis of mitochondrial intermembrane space proteins depends on specific machinery that transfers disulfide bonds to precursor proteins. The machinery shares features with protein relays for disulfide bond formation in the bacterial periplasm and endoplasmic reticulum. A disulfide-generating enzyme/sulfhydryl oxidase oxidizes a disulfide carrier protein, which in turn transfers a disulfide to the substrate protein. Current views suggest that the disulfide carrier alternates between binding to the oxidase and the substrate. We have analyzed the cooperation of the disulfide relay components during import of precursors into mitochondria and identified a ternary complex of all three components. The ternary complex represents a transient and intermediate step in the oxidation of intermembrane space precursors, where the oxidase Erv1 promotes disulfide transfer to the precursor while both oxidase and precursor are associated with the disulfide carrier Mia40.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Cell Biol</journal-id><journal-id journal-id-type="publisher-id">jcb</journal-id><journal-title>The Journal of Cell Biology</journal-title><issn pub-type="ppub">0021-9525</issn><issn pub-type="epub">1540-8140</issn><publisher><publisher-name>The Rockefeller University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18852299</article-id><article-id pub-id-type="pmc">2568017</article-id><article-id pub-id-type="publisher-id">200804095</article-id><article-id pub-id-type="doi">10.1083/jcb.200804095</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject><subj-group><subject>Report</subject></subj-group></subj-group></article-categories><title-group><article-title>Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Stojanovski</surname><given-names>Diana</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref></contrib><contrib contrib-type="author"><name><surname>Milenkovic</surname><given-names>Dusanka</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref></contrib><contrib contrib-type="author"><name><surname>Müller</surname><given-names>Judith M.</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref></contrib><contrib contrib-type="author"><name><surname>Gabriel</surname><given-names>Kipros</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref></contrib><contrib contrib-type="author"><name><surname>Schulze-Specking</surname><given-names>Agnes</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref></contrib><contrib contrib-type="author"><name><surname>Baker</surname><given-names>Michael J.</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Ryan</surname><given-names>Michael T.</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Guiard</surname><given-names>Bernard</given-names></name><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Pfanner</surname><given-names>Nikolaus</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">2</xref></contrib><contrib contrib-type="author"><name><surname>Chacinska</surname><given-names>Agnieszka</given-names></name><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">1</xref><xref ref-type="aff" rid="N0x3a3fdd0N0x400fa70">2</xref></contrib></contrib-group><aff id="N0x3a3fdd0N0x400fa70"><label>1</label>Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, and<label>2</label>Centre for Biological Signalling Studies (<italic>bioss</italic>), Universität Freiburg, 79104 Freiburg, Germany</aff><aff id="aff3"><label>3</label>Department of Biochemistry, La Trobe University, Melbourne 3086, Australia</aff><aff id="aff4"><label>4</label>Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France</aff><author-notes><fn><p>Correspondence to Nikolaus Pfanner: <email>nikolaus.pfanner@biochemie.uni-freiburg.de</email>; or Agnieszka Chacinska: <email>agnieszka.chacinska@biochemie.uni-freiburg.de</email></p></fn></author-notes><pub-date pub-type="ppub"><day>20</day><month>10</month><year>2008</year></pub-date><volume>183</volume><issue>2</issue><fpage>195</fpage><lpage>202</lpage><history><date date-type="received"><day>17</day><month>4</month><year>2008</year></date><date date-type="accepted"><day>15</day><month>9</month><year>2008</year></date></history><permissions><copyright-statement>© 2008 Stojanovski et al.</copyright-statement><license><p>This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see <ext-link ext-link-type="uri" xlink:href="http://www.jcb.org/misc/terms.shtml">http://www.jcb.org/misc/terms.shtml</ext-link>). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">http://creativecommons.org/licenses/by-nc-sa/3.0/</ext-link>).</p></license></permissions><self-uri xlink:title="pdf" xlink:href="jcb1830195.pdf"></self-uri><abstract><p>The biogenesis of mitochondrial intermembrane space proteins depends on specific machinery that transfers disulfide bonds to precursor proteins. The machinery shares features with protein relays for disulfide bond formation in the bacterial periplasm and endoplasmic reticulum. A disulfide-generating enzyme/sulfhydryl oxidase oxidizes a disulfide carrier protein, which in turn transfers a disulfide to the substrate protein. Current views suggest that the disulfide carrier alternates between binding to the oxidase and the substrate. We have analyzed the cooperation of the disulfide relay components during import of precursors into mitochondria and identified a ternary complex of all three components. The ternary complex represents a transient and intermediate step in the oxidation of intermembrane space precursors, where the oxidase Erv1 promotes disulfide transfer to the precursor while both oxidase and precursor are associated with the disulfide carrier Mia40.</p></abstract></article-meta><notes><fn-group><fn><p>D. Stojanovski and D. Milenkovic contributed equally to this paper.</p></fn><fn><p>K. Gabriel's present address is Department of Genetics, University of Melbourne, Parkville 3010, Australia</p></fn><fn><p>Abbreviations used in this paper: AMS, 4-acetamido-4′-maleimidylstilbene-2,2′-disulfonic acid; Dsb, disulfide bond formation protein; IMS, intermembrane space; MIA, mitochondrial IMS import and assembly; Tim, translocase of the inner mitochondrial membrane.</p></fn></fn-group></notes></front></pmc><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>France</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li><li>Victoria (État)</li></region><settlement><li>Fribourg-en-Brisgau</li><li>Melbourne</li></settlement></list><tree><noCountry><name sortKey="Gabriel, Kipros" sort="Gabriel, Kipros" uniqKey="Gabriel K" first="Kipros" last="Gabriel">Kipros Gabriel</name><name sortKey="Milenkovic, Dusanka" sort="Milenkovic, Dusanka" uniqKey="Milenkovic D" first="Dusanka" last="Milenkovic">Dusanka Milenkovic</name><name sortKey="Muller, Judith M" sort="Muller, Judith M" uniqKey="Muller J" first="Judith M." last="Müller">Judith M. Müller</name><name sortKey="Schulze Specking, Agnes" sort="Schulze Specking, Agnes" uniqKey="Schulze Specking A" first="Agnes" last="Schulze-Specking">Agnes Schulze-Specking</name><name sortKey="Stojanovski, Diana" sort="Stojanovski, Diana" uniqKey="Stojanovski D" first="Diana" last="Stojanovski">Diana Stojanovski</name></noCountry><country name="Australie"><region name="Victoria (État)"><name sortKey="Baker, Michael J" sort="Baker, Michael J" uniqKey="Baker M" first="Michael J." last="Baker">Michael J. Baker</name></region><name sortKey="Ryan, Michael T" sort="Ryan, Michael T" uniqKey="Ryan M" first="Michael T." last="Ryan">Michael T. Ryan</name></country><country name="France"><noRegion><name sortKey="Guiard, Bernard" sort="Guiard, Bernard" uniqKey="Guiard B" first="Bernard" last="Guiard">Bernard Guiard</name></noRegion></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Pfanner, Nikolaus" sort="Pfanner, Nikolaus" uniqKey="Pfanner N" first="Nikolaus" last="Pfanner">Nikolaus Pfanner</name></region><name sortKey="Chacinska, Agnieszka" sort="Chacinska, Agnieszka" uniqKey="Chacinska A" first="Agnieszka" last="Chacinska">Agnieszka Chacinska</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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