Muscle weakness in TPM3-myopathy is due to reduced Ca2+-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres
Identifieur interne : 000E49 ( Pmc/Checkpoint ); précédent : 000E48; suivant : 000E50Muscle weakness in TPM3-myopathy is due to reduced Ca2+-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres
Auteurs : Michaela Yuen [Australie] ; Sandra T. Cooper [Australie] ; Steve B. Marston [Royaume-Uni] ; Kristen J. Nowak [Australie] ; Elyshia Mcnamara [Australie] ; Nancy Mokbel [Australie, Liban] ; Biljana Ilkovski [Australie] ; Gianina Ravenscroft [Australie] ; John Rendu [France] ; Josine M. De Winter [Pays-Bas] ; Lars Klinge [Allemagne] ; Alan H. Beggs [États-Unis] ; Kathryn N. North [Australie] ; Coen A. C. Ottenheijm [Pays-Bas] ; Nigel F. Clarke [Australie]Source :
- Human Molecular Genetics [ 0964-6906 ] ; 2015.
Abstract
Dominant mutations in
Url:
DOI: 10.1093/hmg/ddv334
PubMed: 26307083
PubMed Central: 4614700
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4614700Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Muscle weakness in <italic>TPM3</italic>
-myopathy is due to reduced Ca<sup>2+</sup>
-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres</title>
<author><name sortKey="Yuen, Michaela" sort="Yuen, Michaela" uniqKey="Yuen M" first="Michaela" last="Yuen">Michaela Yuen</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
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,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Cooper, Sandra T" sort="Cooper, Sandra T" uniqKey="Cooper S" first="Sandra T." last="Cooper">Sandra T. Cooper</name>
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<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
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,<addr-line>Sydney</addr-line>
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,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
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<author><name sortKey="Marston, Steve B" sort="Marston, Steve B" uniqKey="Marston S" first="Steve B." last="Marston">Steve B. Marston</name>
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,<addr-line>London</addr-line>
,<country>UK</country>
,</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
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<author><name sortKey="Nowak, Kristen J" sort="Nowak, Kristen J" uniqKey="Nowak K" first="Kristen J." last="Nowak">Kristen J. Nowak</name>
<affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Harry Perkins Institute of Medical Research and the Centre for Medical Research</addr-line>
,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
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,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Mcnamara, Elyshia" sort="Mcnamara, Elyshia" uniqKey="Mcnamara E" first="Elyshia" last="Mcnamara">Elyshia Mcnamara</name>
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,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Mokbel, Nancy" sort="Mokbel, Nancy" uniqKey="Mokbel N" first="Nancy" last="Mokbel">Nancy Mokbel</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Faculty of Health Sciences, St. George Health Complex</addr-line>
,<institution>The University of Balamand</institution>
,<addr-line>Beirut</addr-line>
,<country>Lebanon</country>
,</nlm:aff>
<country xml:lang="fr">Liban</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ilkovski, Biljana" sort="Ilkovski, Biljana" uniqKey="Ilkovski B" first="Biljana" last="Ilkovski">Biljana Ilkovski</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Ravenscroft, Gianina" sort="Ravenscroft, Gianina" uniqKey="Ravenscroft G" first="Gianina" last="Ravenscroft">Gianina Ravenscroft</name>
<affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Harry Perkins Institute of Medical Research and the Centre for Medical Research</addr-line>
,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Rendu, John" sort="Rendu, John" uniqKey="Rendu J" first="John" last="Rendu">John Rendu</name>
<affiliation wicri:level="1"><nlm:aff id="af6"><addr-line>Département de Biochimie Toxicologie et Pharmacologie, Département de Biochimie Génétique et Moléculaire</addr-line>
,<institution>Centre Hospitalier Universitaire de Grenoble</institution>
,<addr-line>Grenoble</addr-line>
,<country>France</country>
,</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="De Winter, Josine M" sort="De Winter, Josine M" uniqKey="De Winter J" first="Josine M." last="De Winter">Josine M. De Winter</name>
<affiliation wicri:level="1"><nlm:aff id="af7"><addr-line>Department of Physiology</addr-line>
,<institution>Institute for Cardiovascular Research, VU University Medical Center</institution>
,<addr-line>Amsterdam</addr-line>
,<country>The Netherlands</country>
,</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Klinge, Lars" sort="Klinge, Lars" uniqKey="Klinge L" first="Lars" last="Klinge">Lars Klinge</name>
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,<institution>Georg August University</institution>
,<addr-line>Göttingen</addr-line>
,<country>Germany</country>
,</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Beggs, Alan H" sort="Beggs, Alan H" uniqKey="Beggs A" first="Alan H." last="Beggs">Alan H. Beggs</name>
<affiliation wicri:level="1"><nlm:aff id="af9"><addr-line>Division of Genetics and Genomics, The Manton Center for Orphan Disease Research</addr-line>
,<institution>Boston Children's Hospital, Harvard Medical School</institution>
,<addr-line>Boston, MA</addr-line>
,<country>USA</country>
,</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
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<author><name sortKey="North, Kathryn N" sort="North, Kathryn N" uniqKey="North K" first="Kathryn N." last="North">Kathryn N. North</name>
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<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="af10"><institution>Murdoch Children's Research Institute, the Royal Children's Hospital</institution>
,<addr-line>Parkville</addr-line>
,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af11"><addr-line>Department of Paediatrics</addr-line>
,<institution>University of Melbourne</institution>
,<addr-line>Melbourne</addr-line>
,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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</author>
<author><name sortKey="Ottenheijm, Coen A C" sort="Ottenheijm, Coen A C" uniqKey="Ottenheijm C" first="Coen A. C." last="Ottenheijm">Coen A. C. Ottenheijm</name>
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,<institution>Institute for Cardiovascular Research, VU University Medical Center</institution>
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,<country>The Netherlands</country>
,</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Clarke, Nigel F" sort="Clarke, Nigel F" uniqKey="Clarke N" first="Nigel F." last="Clarke">Nigel F. Clarke</name>
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<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Muscle weakness in <italic>TPM3</italic>
-myopathy is due to reduced Ca<sup>2+</sup>
-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres</title>
<author><name sortKey="Yuen, Michaela" sort="Yuen, Michaela" uniqKey="Yuen M" first="Michaela" last="Yuen">Michaela Yuen</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cooper, Sandra T" sort="Cooper, Sandra T" uniqKey="Cooper S" first="Sandra T." last="Cooper">Sandra T. Cooper</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marston, Steve B" sort="Marston, Steve B" uniqKey="Marston S" first="Steve B." last="Marston">Steve B. Marston</name>
<affiliation wicri:level="1"><nlm:aff id="af3"><institution>National Heart and Lung Institute, Imperial College London</institution>
,<addr-line>London</addr-line>
,<country>UK</country>
,</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nowak, Kristen J" sort="Nowak, Kristen J" uniqKey="Nowak K" first="Kristen J." last="Nowak">Kristen J. Nowak</name>
<affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Harry Perkins Institute of Medical Research and the Centre for Medical Research</addr-line>
,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mcnamara, Elyshia" sort="Mcnamara, Elyshia" uniqKey="Mcnamara E" first="Elyshia" last="Mcnamara">Elyshia Mcnamara</name>
<affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Harry Perkins Institute of Medical Research and the Centre for Medical Research</addr-line>
,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mokbel, Nancy" sort="Mokbel, Nancy" uniqKey="Mokbel N" first="Nancy" last="Mokbel">Nancy Mokbel</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Faculty of Health Sciences, St. George Health Complex</addr-line>
,<institution>The University of Balamand</institution>
,<addr-line>Beirut</addr-line>
,<country>Lebanon</country>
,</nlm:aff>
<country xml:lang="fr">Liban</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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</author>
<author><name sortKey="Ilkovski, Biljana" sort="Ilkovski, Biljana" uniqKey="Ilkovski B" first="Biljana" last="Ilkovski">Biljana Ilkovski</name>
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<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ravenscroft, Gianina" sort="Ravenscroft, Gianina" uniqKey="Ravenscroft G" first="Gianina" last="Ravenscroft">Gianina Ravenscroft</name>
<affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Harry Perkins Institute of Medical Research and the Centre for Medical Research</addr-line>
,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rendu, John" sort="Rendu, John" uniqKey="Rendu J" first="John" last="Rendu">John Rendu</name>
<affiliation wicri:level="1"><nlm:aff id="af6"><addr-line>Département de Biochimie Toxicologie et Pharmacologie, Département de Biochimie Génétique et Moléculaire</addr-line>
,<institution>Centre Hospitalier Universitaire de Grenoble</institution>
,<addr-line>Grenoble</addr-line>
,<country>France</country>
,</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="De Winter, Josine M" sort="De Winter, Josine M" uniqKey="De Winter J" first="Josine M." last="De Winter">Josine M. De Winter</name>
<affiliation wicri:level="1"><nlm:aff id="af7"><addr-line>Department of Physiology</addr-line>
,<institution>Institute for Cardiovascular Research, VU University Medical Center</institution>
,<addr-line>Amsterdam</addr-line>
,<country>The Netherlands</country>
,</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Klinge, Lars" sort="Klinge, Lars" uniqKey="Klinge L" first="Lars" last="Klinge">Lars Klinge</name>
<affiliation wicri:level="1"><nlm:aff id="af8"><addr-line>Department of Pediatrics and Adolescent Medicine, Division of Pediatric Neurology, Faculty of Medicine</addr-line>
,<institution>Georg August University</institution>
,<addr-line>Göttingen</addr-line>
,<country>Germany</country>
,</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Beggs, Alan H" sort="Beggs, Alan H" uniqKey="Beggs A" first="Alan H." last="Beggs">Alan H. Beggs</name>
<affiliation wicri:level="1"><nlm:aff id="af9"><addr-line>Division of Genetics and Genomics, The Manton Center for Orphan Disease Research</addr-line>
,<institution>Boston Children's Hospital, Harvard Medical School</institution>
,<addr-line>Boston, MA</addr-line>
,<country>USA</country>
,</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="North, Kathryn N" sort="North, Kathryn N" uniqKey="North K" first="Kathryn N." last="North">Kathryn N. North</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="af10"><institution>Murdoch Children's Research Institute, the Royal Children's Hospital</institution>
,<addr-line>Parkville</addr-line>
,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af11"><addr-line>Department of Paediatrics</addr-line>
,<institution>University of Melbourne</institution>
,<addr-line>Melbourne</addr-line>
,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ottenheijm, Coen A C" sort="Ottenheijm, Coen A C" uniqKey="Ottenheijm C" first="Coen A. C." last="Ottenheijm">Coen A. C. Ottenheijm</name>
<affiliation wicri:level="1"><nlm:aff id="af7"><addr-line>Department of Physiology</addr-line>
,<institution>Institute for Cardiovascular Research, VU University Medical Center</institution>
,<addr-line>Amsterdam</addr-line>
,<country>The Netherlands</country>
,</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Clarke, Nigel F" sort="Clarke, Nigel F" uniqKey="Clarke N" first="Nigel F." last="Clarke">Nigel F. Clarke</name>
<affiliation wicri:level="1"><nlm:aff id="af1"><institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Human Molecular Genetics</title>
<idno type="ISSN">0964-6906</idno>
<idno type="eISSN">1460-2083</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Dominant mutations in <italic>TPM3</italic>
, encoding α-tropomyosin<sub>slow</sub>
, cause a congenital myopathy characterized by generalized muscle weakness. Here, we used a multidisciplinary approach to investigate the mechanism of muscle dysfunction in 12 <italic>TPM3</italic>
-myopathy patients. We confirm that slow myofibre hypotrophy is a diagnostic hallmark of <italic>TPM3</italic>
-myopathy, and is commonly accompanied by skewing of fibre-type ratios (either slow or fast fibre predominance). Patient muscle contained normal ratios of the three tropomyosin isoforms and normal fibre-type expression of myosins and troponins. Using 2D-PAGE, we demonstrate that mutant α-tropomyosin<sub>slow</sub>
was expressed, suggesting muscle dysfunction is due to a dominant-negative effect of mutant protein on muscle contraction. Molecular modelling suggested mutant α-tropomyosin<sub>slow</sub>
likely impacts actin–tropomyosin interactions and, indeed, co-sedimentation assays showed reduced binding of mutant α-tropomyosin<sub>slow</sub>
(R168C) to filamentous actin. Single fibre contractility studies of patient myofibres revealed marked slow myofibre specific abnormalities. At saturating [Ca<sup>2+</sup>
] (pCa 4.5), patient slow fibres produced only 63% of the contractile force produced in control slow fibres and had reduced acto-myosin cross-bridge cycling kinetics. Importantly, due to reduced Ca<sup>2+</sup>
-sensitivity, at sub-saturating [Ca<sup>2+</sup>
] (pCa 6, levels typically released during <italic>in vivo</italic>
contraction) patient slow fibres produced only 26% of the force generated by control slow fibres. Thus, weakness in <italic>TPM3</italic>
-myopathy patients can be directly attributed to reduced slow fibre force at physiological [Ca<sup>2+</sup>
], and impaired acto-myosin cross-bridge cycling kinetics. Fast myofibres are spared; however, they appear to be unable to compensate for slow fibre dysfunction. Abnormal Ca<sup>2+</sup>
-sensitivity in <italic>TPM3</italic>
-myopathy patients suggests Ca<sup>2+</sup>
-sensitizing drugs may represent a useful treatment for this condition.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Hum Mol Genet</journal-id>
<journal-id journal-id-type="iso-abbrev">Hum. Mol. Genet</journal-id>
<journal-id journal-id-type="publisher-id">hmg</journal-id>
<journal-id journal-id-type="hwp">hmg</journal-id>
<journal-title-group><journal-title>Human Molecular Genetics</journal-title>
</journal-title-group>
<issn pub-type="ppub">0964-6906</issn>
<issn pub-type="epub">1460-2083</issn>
<publisher><publisher-name>Oxford University Press</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26307083</article-id>
<article-id pub-id-type="pmc">4614700</article-id>
<article-id pub-id-type="doi">10.1093/hmg/ddv334</article-id>
<article-id pub-id-type="publisher-id">ddv334</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Muscle weakness in <italic>TPM3</italic>
-myopathy is due to reduced Ca<sup>2+</sup>
-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Yuen</surname>
<given-names>Michaela</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="aff" rid="af2">2</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cooper</surname>
<given-names>Sandra T.</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="aff" rid="af2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Marston</surname>
<given-names>Steve B.</given-names>
</name>
<xref ref-type="aff" rid="af3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nowak</surname>
<given-names>Kristen J.</given-names>
</name>
<xref ref-type="aff" rid="af4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>McNamara</surname>
<given-names>Elyshia</given-names>
</name>
<xref ref-type="aff" rid="af4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mokbel</surname>
<given-names>Nancy</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="aff" rid="af5">5</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ilkovski</surname>
<given-names>Biljana</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ravenscroft</surname>
<given-names>Gianina</given-names>
</name>
<xref ref-type="aff" rid="af4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rendu</surname>
<given-names>John</given-names>
</name>
<xref ref-type="aff" rid="af6">6</xref>
</contrib>
<contrib contrib-type="author"><name><surname>de Winter</surname>
<given-names>Josine M.</given-names>
</name>
<xref ref-type="aff" rid="af7">7</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Klinge</surname>
<given-names>Lars</given-names>
</name>
<xref ref-type="aff" rid="af8">8</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Beggs</surname>
<given-names>Alan H.</given-names>
</name>
<xref ref-type="aff" rid="af9">9</xref>
</contrib>
<contrib contrib-type="author"><name><surname>North</surname>
<given-names>Kathryn N.</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="aff" rid="af2">2</xref>
<xref ref-type="aff" rid="af10">10</xref>
<xref ref-type="aff" rid="af11">11</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ottenheijm</surname>
<given-names>Coen A.C.</given-names>
</name>
<xref ref-type="aff" rid="af7">7</xref>
<xref ref-type="author-notes" rid="AN1">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Clarke</surname>
<given-names>Nigel F.</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="aff" rid="af2">2</xref>
<xref ref-type="author-notes" rid="AN1">†</xref>
</contrib>
<aff id="af1"><label>1</label>
<institution>Institute for Neuroscience and</institution>
<institution>Muscle Research, The Children's Hospital at Westmead</institution>
,<addr-line>Westmead</addr-line>
,<country>Australia</country>
,</aff>
<aff id="af2"><label>2</label>
<addr-line>Discipline of Paediatrics and Child Health</addr-line>
,<institution>University of Sydney</institution>
,<addr-line>Sydney</addr-line>
,<country>Australia</country>
,</aff>
<aff id="af3"><label>3</label>
<institution>National Heart and Lung Institute, Imperial College London</institution>
,<addr-line>London</addr-line>
,<country>UK</country>
,</aff>
<aff id="af4"><label>4</label>
<addr-line>Harry Perkins Institute of Medical Research and the Centre for Medical Research</addr-line>
,<institution>University of Western Australia</institution>
,<addr-line>Nedlands</addr-line>
,<country>Australia</country>
,</aff>
<aff id="af5"><label>5</label>
<addr-line>Faculty of Health Sciences, St. George Health Complex</addr-line>
,<institution>The University of Balamand</institution>
,<addr-line>Beirut</addr-line>
,<country>Lebanon</country>
,</aff>
<aff id="af6"><label>6</label>
<addr-line>Département de Biochimie Toxicologie et Pharmacologie, Département de Biochimie Génétique et Moléculaire</addr-line>
,<institution>Centre Hospitalier Universitaire de Grenoble</institution>
,<addr-line>Grenoble</addr-line>
,<country>France</country>
,</aff>
<aff id="af7"><label>7</label>
<addr-line>Department of Physiology</addr-line>
,<institution>Institute for Cardiovascular Research, VU University Medical Center</institution>
,<addr-line>Amsterdam</addr-line>
,<country>The Netherlands</country>
,</aff>
<aff id="af8"><label>8</label>
<addr-line>Department of Pediatrics and Adolescent Medicine, Division of Pediatric Neurology, Faculty of Medicine</addr-line>
,<institution>Georg August University</institution>
,<addr-line>Göttingen</addr-line>
,<country>Germany</country>
,</aff>
<aff id="af9"><label>9</label>
<addr-line>Division of Genetics and Genomics, The Manton Center for Orphan Disease Research</addr-line>
,<institution>Boston Children's Hospital, Harvard Medical School</institution>
,<addr-line>Boston, MA</addr-line>
,<country>USA</country>
,</aff>
<aff id="af10"><label>10</label>
<institution>Murdoch Children's Research Institute, the Royal Children's Hospital</institution>
,<addr-line>Parkville</addr-line>
,<country>Australia</country>
and</aff>
<aff id="af11"><label>11</label>
<addr-line>Department of Paediatrics</addr-line>
,<institution>University of Melbourne</institution>
,<addr-line>Melbourne</addr-line>
,<country>Australia</country>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>*</label>
To whom correspondence should be addressed at: Institute for Neuroscience and Muscle Research, The Children's Hospital at Westmead, Locked Bag 4001, Westmead, NSW 2145, Australia. Tel: +61 298451495; Fax: +61 298453489; Email: <email>michaela.kreissl@sydney.edu.au</email>
</corresp>
<fn id="AN1" fn-type="con"><label>†</label>
<p>The authors wish it to be known that, in their opinion, the last two authors should be regarded as joint last authors.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><day>15</day>
<month>11</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub"><day>24</day>
<month>8</month>
<year>2015</year>
</pub-date>
<volume>24</volume>
<issue>22</issue>
<fpage>6278</fpage>
<lpage>6292</lpage>
<history><date date-type="received"><day>28</day>
<month>5</month>
<year>2015</year>
</date>
<date date-type="rev-recd"><day>20</day>
<month>7</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>10</day>
<month>8</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com</copyright-statement>
<copyright-year>2015</copyright-year>
</permissions>
<self-uri content-type="pdf" xlink:href="ddv334.pdf"></self-uri>
<abstract><p>Dominant mutations in <italic>TPM3</italic>
, encoding α-tropomyosin<sub>slow</sub>
, cause a congenital myopathy characterized by generalized muscle weakness. Here, we used a multidisciplinary approach to investigate the mechanism of muscle dysfunction in 12 <italic>TPM3</italic>
-myopathy patients. We confirm that slow myofibre hypotrophy is a diagnostic hallmark of <italic>TPM3</italic>
-myopathy, and is commonly accompanied by skewing of fibre-type ratios (either slow or fast fibre predominance). Patient muscle contained normal ratios of the three tropomyosin isoforms and normal fibre-type expression of myosins and troponins. Using 2D-PAGE, we demonstrate that mutant α-tropomyosin<sub>slow</sub>
was expressed, suggesting muscle dysfunction is due to a dominant-negative effect of mutant protein on muscle contraction. Molecular modelling suggested mutant α-tropomyosin<sub>slow</sub>
likely impacts actin–tropomyosin interactions and, indeed, co-sedimentation assays showed reduced binding of mutant α-tropomyosin<sub>slow</sub>
(R168C) to filamentous actin. Single fibre contractility studies of patient myofibres revealed marked slow myofibre specific abnormalities. At saturating [Ca<sup>2+</sup>
] (pCa 4.5), patient slow fibres produced only 63% of the contractile force produced in control slow fibres and had reduced acto-myosin cross-bridge cycling kinetics. Importantly, due to reduced Ca<sup>2+</sup>
-sensitivity, at sub-saturating [Ca<sup>2+</sup>
] (pCa 6, levels typically released during <italic>in vivo</italic>
contraction) patient slow fibres produced only 26% of the force generated by control slow fibres. Thus, weakness in <italic>TPM3</italic>
-myopathy patients can be directly attributed to reduced slow fibre force at physiological [Ca<sup>2+</sup>
], and impaired acto-myosin cross-bridge cycling kinetics. Fast myofibres are spared; however, they appear to be unable to compensate for slow fibre dysfunction. Abnormal Ca<sup>2+</sup>
-sensitivity in <italic>TPM3</italic>
-myopathy patients suggests Ca<sup>2+</sup>
-sensitizing drugs may represent a useful treatment for this condition.</p>
</abstract>
<funding-group><award-group id="funding-1"><funding-source>National Health and Medical Research Council of Australia</funding-source>
<award-id>APP571287</award-id>
<award-id>APP1022707</award-id>
<award-id>APP1048816</award-id>
<award-id>APP1035955</award-id>
</award-group>
<award-group id="funding-2"><funding-source>National Institutes of Health (USA)</funding-source>
<award-id>R01 HD075802</award-id>
</award-group>
<award-group id="funding-3"><funding-source>Australian Resource Council Future Fellowship</funding-source>
<award-id>FT100100734</award-id>
</award-group>
</funding-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Allemagne</li>
<li>Australie</li>
<li>France</li>
<li>Liban</li>
<li>Pays-Bas</li>
<li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Yuen, Michaela" sort="Yuen, Michaela" uniqKey="Yuen M" first="Michaela" last="Yuen">Michaela Yuen</name>
</noRegion>
<name sortKey="Clarke, Nigel F" sort="Clarke, Nigel F" uniqKey="Clarke N" first="Nigel F." last="Clarke">Nigel F. Clarke</name>
<name sortKey="Clarke, Nigel F" sort="Clarke, Nigel F" uniqKey="Clarke N" first="Nigel F." last="Clarke">Nigel F. Clarke</name>
<name sortKey="Cooper, Sandra T" sort="Cooper, Sandra T" uniqKey="Cooper S" first="Sandra T." last="Cooper">Sandra T. Cooper</name>
<name sortKey="Cooper, Sandra T" sort="Cooper, Sandra T" uniqKey="Cooper S" first="Sandra T." last="Cooper">Sandra T. Cooper</name>
<name sortKey="Ilkovski, Biljana" sort="Ilkovski, Biljana" uniqKey="Ilkovski B" first="Biljana" last="Ilkovski">Biljana Ilkovski</name>
<name sortKey="Mcnamara, Elyshia" sort="Mcnamara, Elyshia" uniqKey="Mcnamara E" first="Elyshia" last="Mcnamara">Elyshia Mcnamara</name>
<name sortKey="Mokbel, Nancy" sort="Mokbel, Nancy" uniqKey="Mokbel N" first="Nancy" last="Mokbel">Nancy Mokbel</name>
<name sortKey="North, Kathryn N" sort="North, Kathryn N" uniqKey="North K" first="Kathryn N." last="North">Kathryn N. North</name>
<name sortKey="North, Kathryn N" sort="North, Kathryn N" uniqKey="North K" first="Kathryn N." last="North">Kathryn N. North</name>
<name sortKey="North, Kathryn N" sort="North, Kathryn N" uniqKey="North K" first="Kathryn N." last="North">Kathryn N. North</name>
<name sortKey="North, Kathryn N" sort="North, Kathryn N" uniqKey="North K" first="Kathryn N." last="North">Kathryn N. North</name>
<name sortKey="Nowak, Kristen J" sort="Nowak, Kristen J" uniqKey="Nowak K" first="Kristen J." last="Nowak">Kristen J. Nowak</name>
<name sortKey="Ravenscroft, Gianina" sort="Ravenscroft, Gianina" uniqKey="Ravenscroft G" first="Gianina" last="Ravenscroft">Gianina Ravenscroft</name>
<name sortKey="Yuen, Michaela" sort="Yuen, Michaela" uniqKey="Yuen M" first="Michaela" last="Yuen">Michaela Yuen</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Marston, Steve B" sort="Marston, Steve B" uniqKey="Marston S" first="Steve B." last="Marston">Steve B. Marston</name>
</noRegion>
</country>
<country name="Liban"><noRegion><name sortKey="Mokbel, Nancy" sort="Mokbel, Nancy" uniqKey="Mokbel N" first="Nancy" last="Mokbel">Nancy Mokbel</name>
</noRegion>
</country>
<country name="France"><noRegion><name sortKey="Rendu, John" sort="Rendu, John" uniqKey="Rendu J" first="John" last="Rendu">John Rendu</name>
</noRegion>
</country>
<country name="Pays-Bas"><noRegion><name sortKey="De Winter, Josine M" sort="De Winter, Josine M" uniqKey="De Winter J" first="Josine M." last="De Winter">Josine M. De Winter</name>
</noRegion>
<name sortKey="Ottenheijm, Coen A C" sort="Ottenheijm, Coen A C" uniqKey="Ottenheijm C" first="Coen A. C." last="Ottenheijm">Coen A. C. Ottenheijm</name>
</country>
<country name="Allemagne"><noRegion><name sortKey="Klinge, Lars" sort="Klinge, Lars" uniqKey="Klinge L" first="Lars" last="Klinge">Lars Klinge</name>
</noRegion>
</country>
<country name="États-Unis"><noRegion><name sortKey="Beggs, Alan H" sort="Beggs, Alan H" uniqKey="Beggs A" first="Alan H." last="Beggs">Alan H. Beggs</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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