SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model
Identifieur interne : 000D30 ( Pmc/Checkpoint ); précédent : 000D29; suivant : 000D31SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model
Auteurs : Stergios Tsartsalis [Suisse] ; Noé Dumas ; Benjamin B. Tournier ; Tien Pham ; Marcelle Moulin-Sallanon [France] ; Marie-Claude Grégoire ; Yves Charnay ; Philippe MilletSource :
- EJNMMI Research [ 2191-219X ] ; 2015.
Abstract
Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.
GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [123I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy,
Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.
The present study demonstrates the feasibility of [123I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.
Url:
DOI: 10.1186/s13550-015-0092-4
PubMed: 25853015
PubMed Central: 4385259
Affiliations:
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<author><name sortKey="Millet, Philippe" sort="Millet, Philippe" uniqKey="Millet P" first="Philippe" last="Millet">Philippe Millet</name>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.</p>
</sec>
<sec><title>Methods</title>
<p>GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [<sup>123</sup>
I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, <italic>in situ</italic>
nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor.</p>
</sec>
<sec><title>Results</title>
<p>Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.</p>
</sec>
<sec><title>Conclusions</title>
<p>The present study demonstrates the feasibility of [<sup>123</sup>
I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.</p>
</sec>
</div>
</front>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">EJNMMI Res</journal-id>
<journal-id journal-id-type="iso-abbrev">EJNMMI Res</journal-id>
<journal-title-group><journal-title>EJNMMI Research</journal-title>
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<issn pub-type="epub">2191-219X</issn>
<publisher><publisher-name>Springer Berlin Heidelberg</publisher-name>
<publisher-loc>Berlin/Heidelberg</publisher-loc>
</publisher>
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<article-meta><article-id pub-id-type="pmid">25853015</article-id>
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<article-id pub-id-type="publisher-id">92</article-id>
<article-id pub-id-type="doi">10.1186/s13550-015-0092-4</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Preliminary Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Tsartsalis</surname>
<given-names>Stergios</given-names>
</name>
<address><email>stergios.tsartsalis@hcuge.ch</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Dumas</surname>
<given-names>Noé</given-names>
</name>
<address><email>noe.dumas@gmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Tournier</surname>
<given-names>Benjamin B</given-names>
</name>
<address><email>benjamin.tournier@hcuge.ch</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pham</surname>
<given-names>Tien</given-names>
</name>
<address><email>tqp@ansto.gov.au</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Moulin-Sallanon</surname>
<given-names>Marcelle</given-names>
</name>
<address><email>marcelle.moulin@inserm.fr</email>
</address>
<xref ref-type="aff" rid="Aff4"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Grégoire</surname>
<given-names>Marie-Claude</given-names>
</name>
<address><email>marie.gregoire@ansto.gov.au</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Charnay</surname>
<given-names>Yves</given-names>
</name>
<address><email>charnay@infomaniak.ch</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Millet</surname>
<given-names>Philippe</given-names>
</name>
<address><email>philippe.millet@hcuge.ch</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1"><label></label>
Vulnerability Biomarkers Unit, Division of General Psychiatry, Department of Mental Health and Psychiatry, University Hospitals of Geneva, Chemin du Petit-Bel-Air 2, CH1225 Geneva, Chêne-Bourg Switzerland</aff>
<aff id="Aff2"><label></label>
Department of Psychiatry, University of Geneva, 1 rue Michel-Servet, CH1211 Geneva 4, Switzerland</aff>
<aff id="Aff3"><label></label>
ANSTO LifeSciences, Australian Nuclear Science and Technology Organisation, New Illawarra Road, Sydney, NSW 2234 Australia</aff>
<aff id="Aff4"><label></label>
INSERM, J. Fourier University, INSERM Unit 1039, Domaine de la Merci, 38700 La Tronche, France</aff>
</contrib-group>
<pub-date pub-type="epub"><day>13</day>
<month>3</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>13</day>
<month>3</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><year>2015</year>
</pub-date>
<volume>5</volume>
<elocation-id>9</elocation-id>
<history><date date-type="received"><day>19</day>
<month>9</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>24</day>
<month>2</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© Tsartsalis et al.; licensee Springer. 2015</copyright-statement>
<license license-type="open-access"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.</p>
</sec>
<sec><title>Methods</title>
<p>GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [<sup>123</sup>
I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, <italic>in situ</italic>
nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor.</p>
</sec>
<sec><title>Results</title>
<p>Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.</p>
</sec>
<sec><title>Conclusions</title>
<p>The present study demonstrates the feasibility of [<sup>123</sup>
I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>TSPO</kwd>
<kwd>CLINDE</kwd>
<kwd>Glioma</kwd>
<kwd>SPECT</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2015</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>France</li>
<li>Suisse</li>
</country>
<region><li>Canton de Genève</li>
</region>
<settlement><li>Genève</li>
</settlement>
<orgName><li>Université de Genève</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Charnay, Yves" sort="Charnay, Yves" uniqKey="Charnay Y" first="Yves" last="Charnay">Yves Charnay</name>
<name sortKey="Dumas, Noe" sort="Dumas, Noe" uniqKey="Dumas N" first="Noé" last="Dumas">Noé Dumas</name>
<name sortKey="Gregoire, Marie Claude" sort="Gregoire, Marie Claude" uniqKey="Gregoire M" first="Marie-Claude" last="Grégoire">Marie-Claude Grégoire</name>
<name sortKey="Millet, Philippe" sort="Millet, Philippe" uniqKey="Millet P" first="Philippe" last="Millet">Philippe Millet</name>
<name sortKey="Pham, Tien" sort="Pham, Tien" uniqKey="Pham T" first="Tien" last="Pham">Tien Pham</name>
<name sortKey="Tournier, Benjamin B" sort="Tournier, Benjamin B" uniqKey="Tournier B" first="Benjamin B" last="Tournier">Benjamin B. Tournier</name>
</noCountry>
<country name="Suisse"><region name="Canton de Genève"><name sortKey="Tsartsalis, Stergios" sort="Tsartsalis, Stergios" uniqKey="Tsartsalis S" first="Stergios" last="Tsartsalis">Stergios Tsartsalis</name>
</region>
</country>
<country name="France"><noRegion><name sortKey="Moulin Sallanon, Marcelle" sort="Moulin Sallanon, Marcelle" uniqKey="Moulin Sallanon M" first="Marcelle" last="Moulin-Sallanon">Marcelle Moulin-Sallanon</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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