Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo
Identifieur interne : 000881 ( Pmc/Checkpoint ); précédent : 000880; suivant : 000882Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo
Auteurs : Leslie Calapre ; Elin S. Gray ; Sandrine Kurdykowski [France] ; Anthony David [France] ; Prue Hart [Australie] ; Pascal Descargues [France] ; Mel ZimanSource :
- BMC Dermatology [ 1471-5945 ] ; 2016.
Abstract
UV radiation induces significant DNA damage in keratinocytes and is a known risk factor for skin carcinogenesis. However, it has been reported previously that repeated and simultaneous exposure to UV and heat stress increases the rate of cutaneous tumour formation in mice. Since constant exposure to high temperatures and UV are often experienced in the environment, the effects of exposure to UV and heat needs to be clearly addressed in human epidermal cells.
In this study, we determined the effects of repeated UVB exposure 1 kJ/m2 followed by heat (39 °C) to human keratinocytes. Normal human ex vivo skin models and primary keratinocytes (NHEK) were exposed once a day to UVB and/or heat stress for four consecutive days. Cells were then assessed for changes in proliferation, apoptosis and gene expression at 2 days post-exposure, to determine the cumulative and persistent effects of UV and/or heat in skin keratinocytes.
Using ex vivo skin models and primary keratinocytes in vitro, we showed that UVB
Our results suggest that UVB-induced p53-mediated cell cycle arrest and apoptosis are reduced in the presence of heat stress, leading to increased survival of DNA damaged cells. Thus, exposure to UVB and heat stress may act synergistically to allow survival of damaged cells, which could have implications for initiation skin carcinogenesis.
The online version of this article (doi:10.1186/s12895-016-0043-4) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12895-016-0043-4
PubMed: 27230291
PubMed Central: 4882820
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4882820Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo</title><author><name sortKey="Calapre, Leslie" sort="Calapre, Leslie" uniqKey="Calapre L" first="Leslie" last="Calapre">Leslie Calapre</name><affiliation><nlm:aff id="Aff1">School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6027 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Gray, Elin S" sort="Gray, Elin S" uniqKey="Gray E" first="Elin S." last="Gray">Elin S. Gray</name><affiliation><nlm:aff id="Aff1">School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6027 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Kurdykowski, Sandrine" sort="Kurdykowski, Sandrine" uniqKey="Kurdykowski S" first="Sandrine" last="Kurdykowski">Sandrine Kurdykowski</name><affiliation wicri:level="3"><nlm:aff id="Aff2">GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="David, Anthony" sort="David, Anthony" uniqKey="David A" first="Anthony" last="David">Anthony David</name><affiliation wicri:level="3"><nlm:aff id="Aff2">GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Hart, Prue" sort="Hart, Prue" uniqKey="Hart P" first="Prue" last="Hart">Prue Hart</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Telethon Kids Institute, University of Western Australia, 100 Roberts Road, Subiaco, Perth, 6008 Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, University of Western Australia, 100 Roberts Road, Subiaco, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Descargues, Pascal" sort="Descargues, Pascal" uniqKey="Descargues P" first="Pascal" last="Descargues">Pascal Descargues</name><affiliation wicri:level="3"><nlm:aff id="Aff2">GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Ziman, Mel" sort="Ziman, Mel" uniqKey="Ziman M" first="Mel" last="Ziman">Mel Ziman</name><affiliation><nlm:aff id="Aff1">School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6027 Australia</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff3">Department of Pathology and Laboratory Medicine, University of Western Australia, Crawley, WA Australia</nlm:aff><wicri:noCountry code="subfield">WA Australia</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27230291</idno><idno type="pmc">4882820</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882820</idno><idno type="RBID">PMC:4882820</idno><idno type="doi">10.1186/s12895-016-0043-4</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000A61</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A61</idno><idno type="wicri:Area/Pmc/Curation">000A38</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A38</idno><idno type="wicri:Area/Pmc/Checkpoint">000881</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000881</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo</title><author><name sortKey="Calapre, Leslie" sort="Calapre, Leslie" uniqKey="Calapre L" first="Leslie" last="Calapre">Leslie Calapre</name><affiliation><nlm:aff id="Aff1">School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6027 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Gray, Elin S" sort="Gray, Elin S" uniqKey="Gray E" first="Elin S." last="Gray">Elin S. Gray</name><affiliation><nlm:aff id="Aff1">School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6027 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Kurdykowski, Sandrine" sort="Kurdykowski, Sandrine" uniqKey="Kurdykowski S" first="Sandrine" last="Kurdykowski">Sandrine Kurdykowski</name><affiliation wicri:level="3"><nlm:aff id="Aff2">GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="David, Anthony" sort="David, Anthony" uniqKey="David A" first="Anthony" last="David">Anthony David</name><affiliation wicri:level="3"><nlm:aff id="Aff2">GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Hart, Prue" sort="Hart, Prue" uniqKey="Hart P" first="Prue" last="Hart">Prue Hart</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Telethon Kids Institute, University of Western Australia, 100 Roberts Road, Subiaco, Perth, 6008 Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, University of Western Australia, 100 Roberts Road, Subiaco, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Descargues, Pascal" sort="Descargues, Pascal" uniqKey="Descargues P" first="Pascal" last="Descargues">Pascal Descargues</name><affiliation wicri:level="3"><nlm:aff id="Aff2">GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Ziman, Mel" sort="Ziman, Mel" uniqKey="Ziman M" first="Mel" last="Ziman">Mel Ziman</name><affiliation><nlm:aff id="Aff1">School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6027 Australia</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff3">Department of Pathology and Laboratory Medicine, University of Western Australia, Crawley, WA Australia</nlm:aff><wicri:noCountry code="subfield">WA Australia</wicri:noCountry></affiliation></author></analytic><series><title level="j">BMC Dermatology</title><idno type="eISSN">1471-5945</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>UV radiation induces significant DNA damage in keratinocytes and is a known risk factor for skin carcinogenesis. However, it has been reported previously that repeated and simultaneous exposure to UV and heat stress increases the rate of cutaneous tumour formation in mice. Since constant exposure to high temperatures and UV are often experienced in the environment, the effects of exposure to UV and heat needs to be clearly addressed in human epidermal cells.</p></sec><sec><title>Methods</title><p>In this study, we determined the effects of repeated UVB exposure 1 kJ/m<sup>2</sup> followed by heat (39 °C) to human keratinocytes. Normal human ex vivo skin models and primary keratinocytes (NHEK) were exposed once a day to UVB and/or heat stress for four consecutive days. Cells were then assessed for changes in proliferation, apoptosis and gene expression at 2 days post-exposure, to determine the cumulative and persistent effects of UV and/or heat in skin keratinocytes.</p></sec><sec><title>Results</title><p>Using ex vivo skin models and primary keratinocytes in vitro, we showed that UVB <italic>plus</italic> heat treated keratinocytes exhibit persistent DNA damage, as observed with UVB alone. However, we found that apoptosis was significantly reduced in UVB <italic>plus</italic> heat treated samples. Immunohistochemical and whole genome transcription analysis showed that multiple UVB <italic>plus</italic> heat exposures induced inactivation of the p53-mediated stress response. Furthermore, we demonstrated that repeated exposure to UV <italic>plus</italic> heat induced SIRT1 expression and a decrease in acetylated p53 in keratinocytes, which is consistent with the significant downregulation of p53-regulated pro-apoptotic and DNA damage repair genes in these cells.</p></sec><sec><title>Conclusion</title><p>Our results suggest that UVB-induced p53-mediated cell cycle arrest and apoptosis are reduced in the presence of heat stress, leading to increased survival of DNA damaged cells. Thus, exposure to UVB and heat stress may act synergistically to allow survival of damaged cells, which could have implications for initiation skin carcinogenesis.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12895-016-0043-4) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="D Osta, Am" uniqKey="D Osta A">AM D’Costa</name></author><author><name sortKey="Denning, Mf" uniqKey="Denning M">MF Denning</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsunaga, T" uniqKey="Matsunaga T">T Matsunaga</name></author><author><name sortKey="Hieda, K" uniqKey="Hieda K">K Hieda</name></author><author><name sortKey="Nikaido, O" uniqKey="Nikaido O">O Nikaido</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Besarutinia, A" uniqKey="Besarutinia A">A Besarutinia</name></author><author><name sortKey="Bates, Se" uniqKey="Bates S">SE Bates</name></author><author><name sortKey="Synold, Tw" uniqKey="Synold T">TW Synold</name></author><author><name sortKey="Pfeifer, Gp" uniqKey="Pfeifer G">GP Pfeifer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brash, De" uniqKey="Brash D">DE Brash</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Terzian, T" uniqKey="Terzian T">T Terzian</name></author><author><name sortKey="Torchia, E" uniqKey="Torchia E">E Torchia</name></author><author><name sortKey="Dai, D" uniqKey="Dai D">D Dai</name></author><author><name sortKey="Robinson, S" uniqKey="Robinson S">S Robinson</name></author><author><name sortKey="Murao, K" uniqKey="Murao K">K Murao</name></author><author><name sortKey="Steigman, R" uniqKey="Steigman R">R Steigman</name></author><author><name sortKey="Gonzalez, V" uniqKey="Gonzalez V">V Gonzalez</name></author><author><name sortKey="Boyle, G" uniqKey="Boyle G">G Boyle</name></author><author><name sortKey="Powell, M" uniqKey="Powell M">M Powell</name></author><author><name sortKey="Pollock, P" uniqKey="Pollock P">P Pollock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ozaki, T" uniqKey="Ozaki T">T Ozaki</name></author><author><name sortKey="Nakagawara, A" uniqKey="Nakagawara A">A Nakagawara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benjamin, Cl" uniqKey="Benjamin C">CL Benjamin</name></author><author><name sortKey="Ullrich, Se" uniqKey="Ullrich S">SE Ullrich</name></author><author><name sortKey="Kripke, Ml" uniqKey="Kripke M">ML Kripke</name></author><author><name sortKey="Ananthaswamy, Hn" uniqKey="Ananthaswamy H">HN Ananthaswamy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Beckerman, R" uniqKey="Beckerman R">R Beckerman</name></author><author><name sortKey="Prives, C" uniqKey="Prives C">C Prives</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lindahl, T" uniqKey="Lindahl T">T Lindahl</name></author><author><name sortKey="Nyberg, B" uniqKey="Nyberg B">B Nyberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poltev, Vi" uniqKey="Poltev V">VI Poltev</name></author><author><name sortKey="Shulyupina, Nv" uniqKey="Shulyupina N">NV Shulyupina</name></author><author><name sortKey="Bruskov, Vi" uniqKey="Bruskov V">VI Bruskov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bruskov, Vi" uniqKey="Bruskov V">VI Bruskov</name></author><author><name sortKey="Malakhova, Lv" uniqKey="Malakhova L">LV Malakhova</name></author><author><name sortKey="Masalimov, Zk" uniqKey="Masalimov Z">ZK Masalimov</name></author><author><name sortKey="Chernikov, Av" uniqKey="Chernikov A">AV Chernikov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smirnova, Vs" uniqKey="Smirnova V">VS Smirnova</name></author><author><name sortKey="Gudkov, Sv" uniqKey="Gudkov S">SV Gudkov</name></author><author><name sortKey="Chernikov, Av" uniqKey="Chernikov A">AV Chernikov</name></author><author><name sortKey="Bruskov, Vi" uniqKey="Bruskov V">VI Bruskov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takahashi, A" uniqKey="Takahashi A">A Takahashi</name></author><author><name sortKey="Matsumoto, H" uniqKey="Matsumoto H">H Matsumoto</name></author><author><name sortKey="Nagayama, K" uniqKey="Nagayama K">K Nagayama</name></author><author><name sortKey="Kitano, M" uniqKey="Kitano M">M Kitano</name></author><author><name sortKey="Hirose, S" uniqKey="Hirose S">S Hirose</name></author><author><name sortKey="Tanaka, H" uniqKey="Tanaka H">H Tanaka</name></author><author><name sortKey="Mori, E" uniqKey="Mori E">E Mori</name></author><author><name sortKey="Yamakawa, N" uniqKey="Yamakawa N">N Yamakawa</name></author><author><name sortKey="Yasumoto, J" uniqKey="Yasumoto J">J Yasumoto</name></author><author><name sortKey="Yuki, K" uniqKey="Yuki K">K Yuki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ehrlich, M" uniqKey="Ehrlich M">M Ehrlich</name></author><author><name sortKey="Norris, Kf" uniqKey="Norris K">KF Norris</name></author><author><name sortKey="Wang, Ry" uniqKey="Wang R">RY Wang</name></author><author><name sortKey="Kuo, Kc" uniqKey="Kuo K">KC Kuo</name></author><author><name sortKey="Gehrke, Cw" uniqKey="Gehrke C">CW Gehrke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chinnathambi, S" uniqKey="Chinnathambi S">S Chinnathambi</name></author><author><name sortKey="Tomanek Chalkley, A" uniqKey="Tomanek Chalkley A">A Tomanek-Chalkley</name></author><author><name sortKey="Bickenbach, Jr" uniqKey="Bickenbach J">JR Bickenbach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bivik, C" uniqKey="Bivik C">C Bivik</name></author><author><name sortKey="Rosdahl, I" uniqKey="Rosdahl I">I Rosdahl</name></author><author><name sortKey="Ollinger, K" uniqKey="Ollinger K">K Ollinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roti Roti, Jl" uniqKey="Roti Roti J">JL Roti Roti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wong, Rs" uniqKey="Wong R">RS Wong</name></author><author><name sortKey="Kapp, Ln" uniqKey="Kapp L">LN Kapp</name></author><author><name sortKey="Krishnaswamy, G" uniqKey="Krishnaswamy G">G Krishnaswamy</name></author><author><name sortKey="Dewey, Wc" uniqKey="Dewey W">WC Dewey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hunt, Cr" uniqKey="Hunt C">CR Hunt</name></author><author><name sortKey="Pandita, Rk" uniqKey="Pandita R">RK Pandita</name></author><author><name sortKey="Laszlo, A" uniqKey="Laszlo A">A Laszlo</name></author><author><name sortKey="Higashikubo, R" uniqKey="Higashikubo R">R Higashikubo</name></author><author><name sortKey="Agarwal, M" uniqKey="Agarwal M">M Agarwal</name></author><author><name sortKey="Kitamura, T" uniqKey="Kitamura T">T Kitamura</name></author><author><name sortKey="Gupta, A" uniqKey="Gupta A">A Gupta</name></author><author><name sortKey="Rief, N" uniqKey="Rief N">N Rief</name></author><author><name sortKey="Horikoshi, N" uniqKey="Horikoshi N">N Horikoshi</name></author><author><name sortKey="Baskaran, R" uniqKey="Baskaran R">R Baskaran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Maio, A" uniqKey="De Maio A">A De Maio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Maio, A" uniqKey="De Maio A">A De Maio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dai, C" uniqKey="Dai C">C Dai</name></author><author><name sortKey="Dai, S" uniqKey="Dai S">S Dai</name></author><author><name sortKey="Cao, J" uniqKey="Cao J">J Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Akerfelt, M" uniqKey="Akerfelt M">M Akerfelt</name></author><author><name sortKey="Trouillet, D" uniqKey="Trouillet D">D Trouillet</name></author><author><name sortKey="Mezger, V" uniqKey="Mezger V">V Mezger</name></author><author><name sortKey="Sistonen, L" uniqKey="Sistonen L">L Sistonen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Echchgadda, I" uniqKey="Echchgadda I">I Echchgadda</name></author><author><name sortKey="Roth, Cc" uniqKey="Roth C">CC Roth</name></author><author><name sortKey="Cerna, Cz" uniqKey="Cerna C">CZ Cerna</name></author><author><name sortKey="Wilmink, Gj" uniqKey="Wilmink G">GJ Wilmink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Leun, Jc" uniqKey="Van Der Leun J">JC van der Leun</name></author><author><name sortKey="Piacentini, Rd" uniqKey="Piacentini R">RD Piacentini</name></author><author><name sortKey="De Gruijl, Fr" uniqKey="De Gruijl F">FR de Gruijl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Calapre, L" uniqKey="Calapre L">L Calapre</name></author><author><name sortKey="Gray, Es" uniqKey="Gray E">ES Gray</name></author><author><name sortKey="Ziman, M" uniqKey="Ziman M">M Ziman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maytin, Ev" uniqKey="Maytin E">EV Maytin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kane, Ks" uniqKey="Kane K">KS Kane</name></author><author><name sortKey="Maytin, Ev" uniqKey="Maytin E">EV Maytin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trautinger, F" uniqKey="Trautinger F">F Trautinger</name></author><author><name sortKey="Knobler, Rm" uniqKey="Knobler R">RM Knobler</name></author><author><name sortKey="Honigsmann, H" uniqKey="Honigsmann H">H Honigsmann</name></author><author><name sortKey="Mayr, W" uniqKey="Mayr W">W Mayr</name></author><author><name sortKey="Kindas Mugge, I" uniqKey="Kindas Mugge I">I Kindas-Mugge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maytin, Ev" uniqKey="Maytin E">EV Maytin</name></author><author><name sortKey="Murphy, La" uniqKey="Murphy L">LA Murphy</name></author><author><name sortKey="Merrill, Ma" uniqKey="Merrill M">MA Merrill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bain, Ja" uniqKey="Bain J">JA Bain</name></author><author><name sortKey="Rusch, Hp" uniqKey="Rusch H">HP Rusch</name></author><author><name sortKey="Kline, Be" uniqKey="Kline B">BE Kline</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Freeman, Rg" uniqKey="Freeman R">RG Freeman</name></author><author><name sortKey="Knox, Jm" uniqKey="Knox J">JM Knox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dai, C" uniqKey="Dai C">C Dai</name></author><author><name sortKey="Whitesell, L" uniqKey="Whitesell L">L Whitesell</name></author><author><name sortKey="Rogers, Ab" uniqKey="Rogers A">AB Rogers</name></author><author><name sortKey="Lindquist, S" uniqKey="Lindquist S">S Lindquist</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Westerheide, Sd" uniqKey="Westerheide S">SD Westerheide</name></author><author><name sortKey="Anckar, J" uniqKey="Anckar J">J Anckar</name></author><author><name sortKey="Stevens, Sm" uniqKey="Stevens S">SM Stevens</name></author><author><name sortKey="Sistonen, L" uniqKey="Sistonen L">L Sistonen</name></author><author><name sortKey="Morimoto, Ri" uniqKey="Morimoto R">RI Morimoto</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Raynes, R" uniqKey="Raynes R">R Raynes</name></author><author><name sortKey="Pombier, Km" uniqKey="Pombier K">KM Pombier</name></author><author><name sortKey="Nguyen, K" uniqKey="Nguyen K">K Nguyen</name></author><author><name sortKey="Brunquell, J" uniqKey="Brunquell J">J Brunquell</name></author><author><name sortKey="Mendez, Je" uniqKey="Mendez J">JE Mendez</name></author><author><name sortKey="Westerheide, Sd" uniqKey="Westerheide S">SD Westerheide</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Matsumori, H" uniqKey="Matsumori H">H Matsumori</name></author><author><name sortKey="Nakayama, Y" uniqKey="Nakayama Y">Y Nakayama</name></author><author><name sortKey="Osaki, M" uniqKey="Osaki M">M Osaki</name></author><author><name sortKey="Kojima, H" uniqKey="Kojima H">H Kojima</name></author><author><name sortKey="Kurimasa, A" uniqKey="Kurimasa A">A Kurimasa</name></author><author><name sortKey="Ito, H" uniqKey="Ito H">H Ito</name></author><author><name sortKey="Mori, S" uniqKey="Mori S">S Mori</name></author><author><name sortKey="Katoh, M" uniqKey="Katoh M">M Katoh</name></author><author><name sortKey="Oshimura, M" uniqKey="Oshimura M">M Oshimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bratton, Sb" uniqKey="Bratton S">SB Bratton</name></author><author><name sortKey="Salvesen, Gs" uniqKey="Salvesen G">GS Salvesen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cagnol, S" uniqKey="Cagnol S">S Cagnol</name></author><author><name sortKey="Mansour, A" uniqKey="Mansour A">A Mansour</name></author><author><name sortKey="Van Obberghen Schilling, E" uniqKey="Van Obberghen Schilling E">E Van Obberghen-Schilling</name></author><author><name sortKey="Chambard, Jc" uniqKey="Chambard J">JC Chambard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Porter, Ag" uniqKey="Porter A">AG Porter</name></author><author><name sortKey="Janicke, Ru" uniqKey="Janicke R">RU Janicke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bushell, M" uniqKey="Bushell M">M Bushell</name></author><author><name sortKey="Mckendrick, L" uniqKey="Mckendrick L">L McKendrick</name></author><author><name sortKey="Janicke, Ru" uniqKey="Janicke R">RU Janicke</name></author><author><name sortKey="Clemens, Mj" uniqKey="Clemens M">MJ Clemens</name></author><author><name sortKey="Morley, Sj" uniqKey="Morley S">SJ Morley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harris, Sl" uniqKey="Harris S">SL Harris</name></author><author><name sortKey="Levine, Aj" uniqKey="Levine A">AJ Levine</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name></author><author><name sortKey="Fukuda, S" uniqKey="Fukuda S">S Fukuda</name></author><author><name sortKey="Pelus, Lm" uniqKey="Pelus L">LM Pelus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yi, J" uniqKey="Yi J">J Yi</name></author><author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name></author><author><name sortKey="Luo, K" uniqKey="Luo K">K Luo</name></author><author><name sortKey="Liu, T" uniqKey="Liu T">T Liu</name></author><author><name sortKey="Lou, Z" uniqKey="Lou Z">Z Lou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lou, G" uniqKey="Lou G">G Lou</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Wu, S" uniqKey="Wu S">S Wu</name></author><author><name sortKey="Xue, J" uniqKey="Xue J">J Xue</name></author><author><name sortKey="Yang, F" uniqKey="Yang F">F Yang</name></author><author><name sortKey="Fu, H" uniqKey="Fu H">H Fu</name></author><author><name sortKey="Zheng, M" uniqKey="Zheng M">M Zheng</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao, C" uniqKey="Cao C">C Cao</name></author><author><name sortKey="Lu, S" uniqKey="Lu S">S Lu</name></author><author><name sortKey="Kivlin, R" uniqKey="Kivlin R">R Kivlin</name></author><author><name sortKey="Wallin, B" uniqKey="Wallin B">B Wallin</name></author><author><name sortKey="Card, E" uniqKey="Card E">E Card</name></author><author><name sortKey="Bagdasarian, A" uniqKey="Bagdasarian A">A Bagdasarian</name></author><author><name sortKey="Tamakloe, T" uniqKey="Tamakloe T">T Tamakloe</name></author><author><name sortKey="Wang, Wj" uniqKey="Wang W">WJ Wang</name></author><author><name sortKey="Song, X" uniqKey="Song X">X Song</name></author><author><name sortKey="Chu, Wm" uniqKey="Chu W">WM Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chou, Ww" uniqKey="Chou W">WW Chou</name></author><author><name sortKey="Chen, Kc" uniqKey="Chen K">KC Chen</name></author><author><name sortKey="Wang, Ys" uniqKey="Wang Y">YS Wang</name></author><author><name sortKey="Wang, Jy" uniqKey="Wang J">JY Wang</name></author><author><name sortKey="Liang, Cl" uniqKey="Liang C">CL Liang</name></author><author><name sortKey="Juo, Sh" uniqKey="Juo S">SH Juo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jantschitsch, C" uniqKey="Jantschitsch C">C Jantschitsch</name></author><author><name sortKey="Kindas Mugge, I" uniqKey="Kindas Mugge I">I Kindas-Mugge</name></author><author><name sortKey="Metze, D" uniqKey="Metze D">D Metze</name></author><author><name sortKey="Amann, G" uniqKey="Amann G">G Amann</name></author><author><name sortKey="Micksche, M" uniqKey="Micksche M">M Micksche</name></author><author><name sortKey="Trautinger, F" uniqKey="Trautinger F">F Trautinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maytin, Ev" uniqKey="Maytin E">EV Maytin</name></author><author><name sortKey="Wimberly, Jm" uniqKey="Wimberly J">JM Wimberly</name></author><author><name sortKey="Kane, Ks" uniqKey="Kane K">KS Kane</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jantschitsch, C" uniqKey="Jantschitsch C">C Jantschitsch</name></author><author><name sortKey="Trautinger, F" uniqKey="Trautinger F">F Trautinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yogianti, F" uniqKey="Yogianti F">F Yogianti</name></author><author><name sortKey="Kunisada, M" uniqKey="Kunisada M">M Kunisada</name></author><author><name sortKey="Ono, R" uniqKey="Ono R">R Ono</name></author><author><name sortKey="Sakumi, K" uniqKey="Sakumi K">K Sakumi</name></author><author><name sortKey="Nakabeppu, Y" uniqKey="Nakabeppu Y">Y Nakabeppu</name></author><author><name sortKey="Nishigori, C" uniqKey="Nishigori C">C Nishigori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Costin, Ge" uniqKey="Costin G">GE Costin</name></author><author><name sortKey="Hearing, Vj" uniqKey="Hearing V">VJ Hearing</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Dermatol</journal-id><journal-id journal-id-type="iso-abbrev">BMC Dermatol</journal-id><journal-title-group><journal-title>BMC Dermatology</journal-title></journal-title-group><issn pub-type="epub">1471-5945</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27230291</article-id><article-id pub-id-type="pmc">4882820</article-id><article-id pub-id-type="publisher-id">43</article-id><article-id pub-id-type="doi">10.1186/s12895-016-0043-4</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Calapre</surname><given-names>Leslie</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Gray</surname><given-names>Elin S.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Kurdykowski</surname><given-names>Sandrine</given-names></name><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>David</surname><given-names>Anthony</given-names></name><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Hart</surname><given-names>Prue</given-names></name><xref ref-type="aff" rid="Aff4"></xref></contrib><contrib contrib-type="author"><name><surname>Descargues</surname><given-names>Pascal</given-names></name><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Ziman</surname><given-names>Mel</given-names></name><address><phone>+61 8 6304 3640</phone><email>m.ziman@ecu.edu.au</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><aff id="Aff1"><label></label>School of Medical Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027 Australia</aff><aff id="Aff2"><label></label>GENOSKIN Centre Pierre Potier, Oncopole, Toulouse, France</aff><aff id="Aff3"><label></label>Department of Pathology and Laboratory Medicine, University of Western Australia, Crawley, WA Australia</aff><aff id="Aff4"><label></label>Telethon Kids Institute, University of Western Australia, 100 Roberts Road, Subiaco, Perth, 6008 Australia</aff></contrib-group><pub-date pub-type="epub"><day>26</day><month>5</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>26</day><month>5</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>16</volume><elocation-id>6</elocation-id><history><date date-type="received"><day>16</day><month>10</month><year>2015</year></date><date date-type="accepted"><day>18</day><month>5</month><year>2016</year></date></history><permissions><copyright-statement>© The Author(s). 2016</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>UV radiation induces significant DNA damage in keratinocytes and is a known risk factor for skin carcinogenesis. However, it has been reported previously that repeated and simultaneous exposure to UV and heat stress increases the rate of cutaneous tumour formation in mice. Since constant exposure to high temperatures and UV are often experienced in the environment, the effects of exposure to UV and heat needs to be clearly addressed in human epidermal cells.</p></sec><sec><title>Methods</title><p>In this study, we determined the effects of repeated UVB exposure 1 kJ/m<sup>2</sup> followed by heat (39 °C) to human keratinocytes. Normal human ex vivo skin models and primary keratinocytes (NHEK) were exposed once a day to UVB and/or heat stress for four consecutive days. Cells were then assessed for changes in proliferation, apoptosis and gene expression at 2 days post-exposure, to determine the cumulative and persistent effects of UV and/or heat in skin keratinocytes.</p></sec><sec><title>Results</title><p>Using ex vivo skin models and primary keratinocytes in vitro, we showed that UVB <italic>plus</italic> heat treated keratinocytes exhibit persistent DNA damage, as observed with UVB alone. However, we found that apoptosis was significantly reduced in UVB <italic>plus</italic> heat treated samples. Immunohistochemical and whole genome transcription analysis showed that multiple UVB <italic>plus</italic> heat exposures induced inactivation of the p53-mediated stress response. Furthermore, we demonstrated that repeated exposure to UV <italic>plus</italic> heat induced SIRT1 expression and a decrease in acetylated p53 in keratinocytes, which is consistent with the significant downregulation of p53-regulated pro-apoptotic and DNA damage repair genes in these cells.</p></sec><sec><title>Conclusion</title><p>Our results suggest that UVB-induced p53-mediated cell cycle arrest and apoptosis are reduced in the presence of heat stress, leading to increased survival of DNA damaged cells. Thus, exposure to UVB and heat stress may act synergistically to allow survival of damaged cells, which could have implications for initiation skin carcinogenesis.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12895-016-0043-4) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Heat stress</kwd><kwd>UVB</kwd><kwd>Keratinocytes</kwd><kwd>Apoptosis</kwd><kwd>p53</kwd><kwd>DNA damage</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001798</institution-id><institution>Edith Cowan University</institution></institution-wrap></funding-source><award-id>N/A</award-id><principal-award-recipient><name><surname>Ziman</surname><given-names>Mel</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li></region><settlement><li>Toulouse</li></settlement></list><tree><noCountry><name sortKey="Calapre, Leslie" sort="Calapre, Leslie" uniqKey="Calapre L" first="Leslie" last="Calapre">Leslie Calapre</name><name sortKey="Gray, Elin S" sort="Gray, Elin S" uniqKey="Gray E" first="Elin S." last="Gray">Elin S. Gray</name><name sortKey="Ziman, Mel" sort="Ziman, Mel" uniqKey="Ziman M" first="Mel" last="Ziman">Mel Ziman</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Kurdykowski, Sandrine" sort="Kurdykowski, Sandrine" uniqKey="Kurdykowski S" first="Sandrine" last="Kurdykowski">Sandrine Kurdykowski</name></region><name sortKey="David, Anthony" sort="David, Anthony" uniqKey="David A" first="Anthony" last="David">Anthony David</name><name sortKey="Descargues, Pascal" sort="Descargues, Pascal" uniqKey="Descargues P" first="Pascal" last="Descargues">Pascal Descargues</name></country><country name="Australie"><noRegion><name sortKey="Hart, Prue" sort="Hart, Prue" uniqKey="Hart P" first="Prue" last="Hart">Prue Hart</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000881 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000881 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4882820
|texte= Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:27230291" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |