1α,20S-Dihydroxyvitamin D3 Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis
Identifieur interne : 000471 ( Pmc/Checkpoint ); précédent : 000470; suivant : 0004721α,20S-Dihydroxyvitamin D3 Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis
Auteurs : Zongtao Lin [États-Unis] ; Hao Chen [États-Unis] ; Anna Y. Belorusova [France, Suède] ; John C. Bollinger [États-Unis] ; Edith K. Y. Tang ; Zorica Janjetovic [États-Unis] ; Tae-Kang Kim [États-Unis] ; Zhongzhi Wu [États-Unis] ; Duane D. Miller [États-Unis] ; Andrzej T. Slominski [États-Unis] ; Arnold E. Postlethwaite [États-Unis] ; Robert C. Tuckey ; Natacha Rochel [France] ; Wei Li [États-Unis]Source :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
1α,20
Url:
DOI: 10.1038/s41598-017-10917-7
PubMed: 28860545
PubMed Central: 5579064
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:5579064Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">1α,20<italic>S</italic>-Dihydroxyvitamin D<sub>3</sub> Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis</title><author><name sortKey="Lin, Zongtao" sort="Lin, Zongtao" uniqKey="Lin Z" first="Zongtao" last="Lin">Zongtao Lin</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Chen, Hao" sort="Chen, Hao" uniqKey="Chen H" first="Hao" last="Chen">Hao Chen</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Belorusova, Anna Y" sort="Belorusova, Anna Y" uniqKey="Belorusova A" first="Anna Y." last="Belorusova">Anna Y. Belorusova</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI"> 0000 0004 0638 2716</institution-id><institution-id institution-id-type="GRID">grid.420255.4</institution-id><institution>Department of Integrative Structure Biology,</institution><institution>IGBMC - CNRS UMR7104 – Inserm U964, 1, rue Laurent Fries,</institution></institution-wrap>Illkirch, 67400 France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Illkirch</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff9"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1519 6403</institution-id><institution-id institution-id-type="GRID">grid.418151.8</institution-id><institution>Present Address: Department of Medicinal Chemistry,</institution><institution>RIA iMed, AstraZeneca R&D, Pepparedsleden 1,</institution></institution-wrap>S-431 83 Mölndal, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>S-431 83 Mölndal</wicri:regionArea></affiliation></author><author><name sortKey="Bollinger, John C" sort="Bollinger, John C" uniqKey="Bollinger J" first="John C." last="Bollinger">John C. Bollinger</name><affiliation wicri:level="2"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0224 711X</institution-id><institution-id institution-id-type="GRID">grid.240871.8</institution-id><institution>Department of Structural Biology,</institution><institution>St. Jude Children’s Research Hospital,</institution></institution-wrap>Memphis, TN 38105 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Tang, Edith K Y" sort="Tang, Edith K Y" uniqKey="Tang E" first="Edith K. Y." last="Tang">Edith K. Y. Tang</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7910</institution-id><institution-id institution-id-type="GRID">grid.1012.2</institution-id><institution>School of Chemistry and Biochemistry, University of Western Australia,</institution></institution-wrap>Crawley, WA 6009 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6009 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Janjetovic, Zorica" sort="Janjetovic, Zorica" uniqKey="Janjetovic Z" first="Zorica" last="Janjetovic">Zorica Janjetovic</name><affiliation wicri:level="2"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Kim, Tae Kang" sort="Kim, Tae Kang" uniqKey="Kim T" first="Tae-Kang" last="Kim">Tae-Kang Kim</name><affiliation wicri:level="2"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Wu, Zhongzhi" sort="Wu, Zhongzhi" uniqKey="Wu Z" first="Zhongzhi" last="Wu">Zhongzhi Wu</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Miller, Duane D" sort="Miller, Duane D" uniqKey="Miller D" first="Duane D." last="Miller">Duane D. Miller</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Slominski, Andrzej T" sort="Slominski, Andrzej T" uniqKey="Slominski A" first="Andrzej T." last="Slominski">Andrzej T. Slominski</name><affiliation wicri:level="2"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff6"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0419 1326</institution-id><institution-id institution-id-type="GRID">grid.280808.a</institution-id><institution>VA Medical Center,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Postlethwaite, Arnold E" sort="Postlethwaite, Arnold E" uniqKey="Postlethwaite A" first="Arnold E." last="Postlethwaite">Arnold E. Postlethwaite</name><affiliation wicri:level="2"><nlm:aff id="Aff7"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Medicine,</institution><institution>University of Tennessee Health Science Center,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff8"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0420 4721</institution-id><institution-id institution-id-type="GRID">grid.413847.d</institution-id><institution>VA Medical Center,</institution></institution-wrap>Memphis, TN 38104 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Tuckey, Robert C" sort="Tuckey, Robert C" uniqKey="Tuckey R" first="Robert C." last="Tuckey">Robert C. Tuckey</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7910</institution-id><institution-id institution-id-type="GRID">grid.1012.2</institution-id><institution>School of Chemistry and Biochemistry, University of Western Australia,</institution></institution-wrap>Crawley, WA 6009 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6009 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Rochel, Natacha" sort="Rochel, Natacha" uniqKey="Rochel N" first="Natacha" last="Rochel">Natacha Rochel</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI"> 0000 0004 0638 2716</institution-id><institution-id institution-id-type="GRID">grid.420255.4</institution-id><institution>Department of Integrative Structure Biology,</institution><institution>IGBMC - CNRS UMR7104 – Inserm U964, 1, rue Laurent Fries,</institution></institution-wrap>Illkirch, 67400 France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Illkirch</wicri:regionArea></affiliation></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28860545</idno><idno type="pmc">5579064</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579064</idno><idno type="RBID">PMC:5579064</idno><idno type="doi">10.1038/s41598-017-10917-7</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000E36</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E36</idno><idno type="wicri:Area/Pmc/Curation">000C97</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C97</idno><idno type="wicri:Area/Pmc/Checkpoint">000471</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000471</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">1α,20<italic>S</italic>-Dihydroxyvitamin D<sub>3</sub> Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis</title><author><name sortKey="Lin, Zongtao" sort="Lin, Zongtao" uniqKey="Lin Z" first="Zongtao" last="Lin">Zongtao Lin</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Chen, Hao" sort="Chen, Hao" uniqKey="Chen H" first="Hao" last="Chen">Hao Chen</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Belorusova, Anna Y" sort="Belorusova, Anna Y" uniqKey="Belorusova A" first="Anna Y." last="Belorusova">Anna Y. Belorusova</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI"> 0000 0004 0638 2716</institution-id><institution-id institution-id-type="GRID">grid.420255.4</institution-id><institution>Department of Integrative Structure Biology,</institution><institution>IGBMC - CNRS UMR7104 – Inserm U964, 1, rue Laurent Fries,</institution></institution-wrap>Illkirch, 67400 France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Illkirch</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff9"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1519 6403</institution-id><institution-id institution-id-type="GRID">grid.418151.8</institution-id><institution>Present Address: Department of Medicinal Chemistry,</institution><institution>RIA iMed, AstraZeneca R&D, Pepparedsleden 1,</institution></institution-wrap>S-431 83 Mölndal, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>S-431 83 Mölndal</wicri:regionArea></affiliation></author><author><name sortKey="Bollinger, John C" sort="Bollinger, John C" uniqKey="Bollinger J" first="John C." last="Bollinger">John C. Bollinger</name><affiliation wicri:level="2"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0224 711X</institution-id><institution-id institution-id-type="GRID">grid.240871.8</institution-id><institution>Department of Structural Biology,</institution><institution>St. Jude Children’s Research Hospital,</institution></institution-wrap>Memphis, TN 38105 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Tang, Edith K Y" sort="Tang, Edith K Y" uniqKey="Tang E" first="Edith K. Y." last="Tang">Edith K. Y. Tang</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7910</institution-id><institution-id institution-id-type="GRID">grid.1012.2</institution-id><institution>School of Chemistry and Biochemistry, University of Western Australia,</institution></institution-wrap>Crawley, WA 6009 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6009 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Janjetovic, Zorica" sort="Janjetovic, Zorica" uniqKey="Janjetovic Z" first="Zorica" last="Janjetovic">Zorica Janjetovic</name><affiliation wicri:level="2"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Kim, Tae Kang" sort="Kim, Tae Kang" uniqKey="Kim T" first="Tae-Kang" last="Kim">Tae-Kang Kim</name><affiliation wicri:level="2"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Wu, Zhongzhi" sort="Wu, Zhongzhi" uniqKey="Wu Z" first="Zhongzhi" last="Wu">Zhongzhi Wu</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Miller, Duane D" sort="Miller, Duane D" uniqKey="Miller D" first="Duane D." last="Miller">Duane D. Miller</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Slominski, Andrzej T" sort="Slominski, Andrzej T" uniqKey="Slominski A" first="Andrzej T." last="Slominski">Andrzej T. Slominski</name><affiliation wicri:level="2"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff6"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0419 1326</institution-id><institution-id institution-id-type="GRID">grid.280808.a</institution-id><institution>VA Medical Center,</institution></institution-wrap>Birmingham, AL 35294 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Postlethwaite, Arnold E" sort="Postlethwaite, Arnold E" uniqKey="Postlethwaite A" first="Arnold E." last="Postlethwaite">Arnold E. Postlethwaite</name><affiliation wicri:level="2"><nlm:aff id="Aff7"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Medicine,</institution><institution>University of Tennessee Health Science Center,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff8"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0420 4721</institution-id><institution-id institution-id-type="GRID">grid.413847.d</institution-id><institution>VA Medical Center,</institution></institution-wrap>Memphis, TN 38104 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Tuckey, Robert C" sort="Tuckey, Robert C" uniqKey="Tuckey R" first="Robert C." last="Tuckey">Robert C. Tuckey</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7910</institution-id><institution-id institution-id-type="GRID">grid.1012.2</institution-id><institution>School of Chemistry and Biochemistry, University of Western Australia,</institution></institution-wrap>Crawley, WA 6009 Australia</nlm:aff><wicri:noCountry code="subfield">WA 6009 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Rochel, Natacha" sort="Rochel, Natacha" uniqKey="Rochel N" first="Natacha" last="Rochel">Natacha Rochel</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI"> 0000 0004 0638 2716</institution-id><institution-id institution-id-type="GRID">grid.420255.4</institution-id><institution>Department of Integrative Structure Biology,</institution><institution>IGBMC - CNRS UMR7104 – Inserm U964, 1, rue Laurent Fries,</institution></institution-wrap>Illkirch, 67400 France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Illkirch</wicri:regionArea></affiliation></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Memphis</wicri:cityArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">1α,20<italic>S</italic>-Dihydroxyvitamin D3 [1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub>], a natural and bioactive vitamin D3 metabolite, was chemically synthesized for the first time. X-ray crystallography analysis of intermediate 15 confirmed its 1α-OH configuration. 1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub> interacts with the vitamin D receptor (VDR), with similar potency to its native ligand, 1α,25-dihydroxyvitamin D<sub>3</sub> [1,25(OH)<sub>2</sub>D<sub>3</sub>] as illustrated by its ability to stimulate translocation of the VDR to the nucleus, stimulate VDRE-reporter activity, regulate VDR downstream genes (<italic>VDR</italic>, <italic>CYP24A1</italic>, <italic>TRPV6</italic> and <italic>CYP27B1</italic>), and inhibit the production of inflammatory markers (IFNγ and IL1β). However, their co-crystal structures revealed differential molecular interactions of the 20<italic>S</italic>-OH moiety and the 25-OH moiety to the VDR, which may explain some differences in their biological activities. Furthermore, this study provides a synthetic route for the synthesis of 1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub> using the intermediate 1α,3β-diacetoxypregn-5-en-20-one (3), and provides a molecular and biological basis for the development of 1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub> and its analogs as potential therapeutic agents.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lin, Z" uniqKey="Lin Z">Z Lin</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wierzbicka, J" uniqKey="Wierzbicka J">J Wierzbicka</name></author><author><name sortKey="Piotrowska, A" uniqKey="Piotrowska A">A Piotrowska</name></author><author><name sortKey="Zmijewski, Ma" uniqKey="Zmijewski M">MA Zmijewski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haussler, Mr" uniqKey="Haussler M">MR Haussler</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tuckey, Rc" uniqKey="Tuckey R">RC Tuckey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, Z" uniqKey="Lin Z">Z Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, Z" uniqKey="Lin Z">Z Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, Z" uniqKey="Lin Z">Z Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, Yz" uniqKey="Yin Y">YZ Yin</name></author><author><name sortKey="Liu, C" uniqKey="Liu C">C Liu</name></author><author><name sortKey="Tang, Lq" uniqKey="Tang L">LQ Tang</name></author><author><name sortKey="Liu, Zp" uniqKey="Liu Z">ZP Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poza, J" uniqKey="Poza J">J Poza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mouri O, A" uniqKey="Mouri O A">A Mouriño</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otero, R" uniqKey="Otero R">R Otero</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huet, T" uniqKey="Huet T">T Huet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="St Arnaud, R" uniqKey="St Arnaud R">R St-Arnaud</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clapham, De" uniqKey="Clapham D">DE Clapham</name></author><author><name sortKey="Julius, D" uniqKey="Julius D">D Julius</name></author><author><name sortKey="Montell, C" uniqKey="Montell C">C Montell</name></author><author><name sortKey="Schultz, G" uniqKey="Schultz G">G Schultz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holick, Mf" uniqKey="Holick M">MF Holick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zbytek, B" uniqKey="Zbytek B">B Zbytek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pingili, Ak" uniqKey="Pingili A">AK Pingili</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, Z" uniqKey="Lin Z">Z Lin</name></author><author><name sortKey="Yang, R" uniqKey="Yang R">R Yang</name></author><author><name sortKey="Guan, Z" uniqKey="Guan Z">Z Guan</name></author><author><name sortKey="Chen, A" uniqKey="Chen A">A Chen</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huet, T" uniqKey="Huet T">T Huet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otwinowski, Z" uniqKey="Otwinowski Z">Z Otwinowski</name></author><author><name sortKey="Minor, W" uniqKey="Minor W">W Minor</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Adams, Pd" uniqKey="Adams P">PD Adams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P Emsley</name></author><author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, A" uniqKey="Slominski A">A Slominski</name></author><author><name sortKey="Zbytek, B" uniqKey="Zbytek B">B Zbytek</name></author><author><name sortKey="Slominski, R" uniqKey="Slominski R">R Slominski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, T K" uniqKey="Kim T">T-K Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slominski, At" uniqKey="Slominski A">AT Slominski</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28860545</article-id><article-id pub-id-type="pmc">5579064</article-id><article-id pub-id-type="publisher-id">10917</article-id><article-id pub-id-type="doi">10.1038/s41598-017-10917-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>1α,20<italic>S</italic>-Dihydroxyvitamin D<sub>3</sub> Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis</article-title></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-6017-338X</contrib-id><name><surname>Lin</surname><given-names>Zongtao</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Hao</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Belorusova</surname><given-names>Anna Y.</given-names></name><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff9">9</xref></contrib><contrib contrib-type="author"><name><surname>Bollinger</surname><given-names>John C.</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Edith K. Y.</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Janjetovic</surname><given-names>Zorica</given-names></name><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Tae-Kang</given-names></name><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Zhongzhi</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Miller</surname><given-names>Duane D.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-8963-3995</contrib-id><name><surname>Slominski</surname><given-names>Andrzej T.</given-names></name><xref ref-type="aff" rid="Aff5">5</xref><xref ref-type="aff" rid="Aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Postlethwaite</surname><given-names>Arnold E.</given-names></name><xref ref-type="aff" rid="Aff7">7</xref><xref ref-type="aff" rid="Aff8">8</xref></contrib><contrib contrib-type="author"><name><surname>Tuckey</surname><given-names>Robert C.</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Rochel</surname><given-names>Natacha</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Li</surname><given-names>Wei</given-names></name><address><email>wli@uthsc.edu</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Pharmaceutical Sciences,</institution><institution>University of Tennessee Health Science Center, 881 Madison Avenue, Room 561,</institution></institution-wrap>Memphis, TN 38163 United States</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="ISNI"> 0000 0004 0638 2716</institution-id><institution-id institution-id-type="GRID">grid.420255.4</institution-id><institution>Department of Integrative Structure Biology,</institution><institution>IGBMC - CNRS UMR7104 – Inserm U964, 1, rue Laurent Fries,</institution></institution-wrap>Illkirch, 67400 France</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0224 711X</institution-id><institution-id institution-id-type="GRID">grid.240871.8</institution-id><institution>Department of Structural Biology,</institution><institution>St. Jude Children’s Research Hospital,</institution></institution-wrap>Memphis, TN 38105 United States</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7910</institution-id><institution-id institution-id-type="GRID">grid.1012.2</institution-id><institution>School of Chemistry and Biochemistry, University of Western Australia,</institution></institution-wrap>Crawley, WA 6009 Australia</aff><aff id="Aff5"><label>5</label><institution-wrap><institution-id institution-id-type="ISNI">0000000106344187</institution-id><institution-id institution-id-type="GRID">grid.265892.2</institution-id><institution>Department of Dermatology,</institution><institution>University of Alabama at Birmingham,</institution></institution-wrap>Birmingham, AL 35294 United States</aff><aff id="Aff6"><label>6</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0419 1326</institution-id><institution-id institution-id-type="GRID">grid.280808.a</institution-id><institution>VA Medical Center,</institution></institution-wrap>Birmingham, AL 35294 United States</aff><aff id="Aff7"><label>7</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0386 9246</institution-id><institution-id institution-id-type="GRID">grid.267301.1</institution-id><institution>Department of Medicine,</institution><institution>University of Tennessee Health Science Center,</institution></institution-wrap>Memphis, TN 38163 United States</aff><aff id="Aff8"><label>8</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0420 4721</institution-id><institution-id institution-id-type="GRID">grid.413847.d</institution-id><institution>VA Medical Center,</institution></institution-wrap>Memphis, TN 38104 United States</aff><aff id="Aff9"><label>9</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1519 6403</institution-id><institution-id institution-id-type="GRID">grid.418151.8</institution-id><institution>Present Address: Department of Medicinal Chemistry,</institution><institution>RIA iMed, AstraZeneca R&D, Pepparedsleden 1,</institution></institution-wrap>S-431 83 Mölndal, Sweden</aff></contrib-group><pub-date pub-type="epub"><day>31</day><month>8</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>31</day><month>8</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>7</volume><elocation-id>10193</elocation-id><history><date date-type="received"><day>17</day><month>5</month><year>2017</year></date><date date-type="accepted"><day>16</day><month>8</month><year>2017</year></date></history><permissions><copyright-statement>© The Author(s) 2017</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold> This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">1α,20<italic>S</italic>-Dihydroxyvitamin D3 [1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub>], a natural and bioactive vitamin D3 metabolite, was chemically synthesized for the first time. X-ray crystallography analysis of intermediate 15 confirmed its 1α-OH configuration. 1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub> interacts with the vitamin D receptor (VDR), with similar potency to its native ligand, 1α,25-dihydroxyvitamin D<sub>3</sub> [1,25(OH)<sub>2</sub>D<sub>3</sub>] as illustrated by its ability to stimulate translocation of the VDR to the nucleus, stimulate VDRE-reporter activity, regulate VDR downstream genes (<italic>VDR</italic>, <italic>CYP24A1</italic>, <italic>TRPV6</italic> and <italic>CYP27B1</italic>), and inhibit the production of inflammatory markers (IFNγ and IL1β). However, their co-crystal structures revealed differential molecular interactions of the 20<italic>S</italic>-OH moiety and the 25-OH moiety to the VDR, which may explain some differences in their biological activities. Furthermore, this study provides a synthetic route for the synthesis of 1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub> using the intermediate 1α,3β-diacetoxypregn-5-en-20-one (3), and provides a molecular and biological basis for the development of 1,20<italic>S</italic>(OH)<sub>2</sub>D<sub>3</sub> and its analogs as potential therapeutic agents.</p></abstract><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2017</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>France</li><li>Suède</li><li>États-Unis</li></country><region><li>Alabama</li><li>Tennessee</li></region></list><tree><noCountry><name sortKey="Tang, Edith K Y" sort="Tang, Edith K Y" uniqKey="Tang E" first="Edith K. Y." last="Tang">Edith K. Y. Tang</name><name sortKey="Tuckey, Robert C" sort="Tuckey, Robert C" uniqKey="Tuckey R" first="Robert C." last="Tuckey">Robert C. Tuckey</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Lin, Zongtao" sort="Lin, Zongtao" uniqKey="Lin Z" first="Zongtao" last="Lin">Zongtao Lin</name></region><name sortKey="Bollinger, John C" sort="Bollinger, John C" uniqKey="Bollinger J" first="John C." last="Bollinger">John C. Bollinger</name><name sortKey="Chen, Hao" sort="Chen, Hao" uniqKey="Chen H" first="Hao" last="Chen">Hao Chen</name><name sortKey="Janjetovic, Zorica" sort="Janjetovic, Zorica" uniqKey="Janjetovic Z" first="Zorica" last="Janjetovic">Zorica Janjetovic</name><name sortKey="Kim, Tae Kang" sort="Kim, Tae Kang" uniqKey="Kim T" first="Tae-Kang" last="Kim">Tae-Kang Kim</name><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><name sortKey="Miller, Duane D" sort="Miller, Duane D" uniqKey="Miller D" first="Duane D." last="Miller">Duane D. Miller</name><name sortKey="Postlethwaite, Arnold E" sort="Postlethwaite, Arnold E" uniqKey="Postlethwaite A" first="Arnold E." last="Postlethwaite">Arnold E. Postlethwaite</name><name sortKey="Postlethwaite, Arnold E" sort="Postlethwaite, Arnold E" uniqKey="Postlethwaite A" first="Arnold E." last="Postlethwaite">Arnold E. Postlethwaite</name><name sortKey="Slominski, Andrzej T" sort="Slominski, Andrzej T" uniqKey="Slominski A" first="Andrzej T." last="Slominski">Andrzej T. Slominski</name><name sortKey="Slominski, Andrzej T" sort="Slominski, Andrzej T" uniqKey="Slominski A" first="Andrzej T." last="Slominski">Andrzej T. Slominski</name><name sortKey="Wu, Zhongzhi" sort="Wu, Zhongzhi" uniqKey="Wu Z" first="Zhongzhi" last="Wu">Zhongzhi Wu</name></country><country name="France"><noRegion><name sortKey="Belorusova, Anna Y" sort="Belorusova, Anna Y" uniqKey="Belorusova A" first="Anna Y." last="Belorusova">Anna Y. Belorusova</name></noRegion><name sortKey="Rochel, Natacha" sort="Rochel, Natacha" uniqKey="Rochel N" first="Natacha" last="Rochel">Natacha Rochel</name></country><country name="Suède"><noRegion><name sortKey="Belorusova, Anna Y" sort="Belorusova, Anna Y" uniqKey="Belorusova A" first="Anna Y." last="Belorusova">Anna Y. Belorusova</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000471 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000471 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:5579064
|texte= 1α,20S-Dihydroxyvitamin D3 Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:28860545" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |