AustralieFrV1, PascalFrancis, Curation, bibRecord, 004602

acDCs enhance human antigen-specific T-cell responses

Identifieur interne : 004602 ( PascalFrancis/Curation ); précédent : 004601; suivant : 004603

acDCs enhance human antigen-specific T-cell responses

Auteurs : Emanuela Martinuzzi [France] ; Georgia Afonso [France] ; Marie-Claude Gagnerault [France] ; Gaetano Naselli [France] ; Diana Mittag [Australie] ; Béhazine Combadiere [France] ; Christian Boitard [France] ; Nathalie Chaput [France] ; Laurence Zitvogel [France] ; Leonard C. Harrison [Australie] ; Roberto Mallone [France]

Source :

Blood [ 0006-4971 ] ; 2011.

RBID : Pascal:11-0412241

Descripteurs français

Pascal (Inist)
- Homme, Réponse immune, Hématologie, Spécificité antigénique.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Antigen specific, Hematology, Human, Immune response.

Abstract

Detection of human Ag-specific T cells is limited by sensitivity and blood requirements. As dendritic cells (DCs) can potently stimulate T cells, we hypothesized that their induction in PBMCs in situ could link Ag processing and presentation to Ag-specific T-cell activation. To this end, unfractionated PBMCs (fresh or frozen) or whole blood were incubated for 48 hours with protein or peptide Ag together with different DC-activating agents to rapidly and sequentially induce, pulse, and mature DCs. DC activation was therefore lined up with Ag recognition by neighboring T cells, thus telescoping the sequential steps of T-cell activation. Efficient processing of protein Ags made prior knowledge of epitopes and HLA restrictions dispensable. While reducing stimulation time, manipulation and blood requirements, in situ DC induction specifically amplified Ag-specific T-cell responses (cytokine secretion, proliferation, CD137/CD154 up-regulation, and binding of peptide-HLA multimers). IL-1β, although released by DCs, was also secreted in an Ag-specific fashion, thus providing an indirect biomarker of T-cell responses. These accelerated cocultured DC (acDC) assays offered a sensitive means with which to evaluate T-cell responses to viral and melanoma Ag vaccination, and may therefore find application for immune monitoring in viral, tumor, autoimmune, and transplantation settings.

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A08	`01`	`1`	`ENG`	`@1 acDCs enhance human antigen-specific T-cell responses`
A11	`01`	`1`		`@1 MARTINUZZI (Emanuela)`
A11	`02`	`1`		`@1 AFONSO (Georgia)`
A11	`03`	`1`		`@1 GAGNERAULT (Marie-Claude)`
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A11	`06`	`1`		`@1 COMBADIERE (Béhazine)`
A11	`07`	`1`		`@1 BOITARD (Christian)`
A11	`08`	`1`		`@1 CHAPUT (Nathalie)`
A11	`09`	`1`		`@1 ZITVOGEL (Laurence)`
A11	`10`	`1`		`@1 HARRISON (Leonard C.)`
A11	`11`	`1`		`@1 MALLONE (Roberto)`
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A14	`03`			`@1 The Walter and Eliza Hall Institute of Medical Research, Parkville @2 Victoria @3 AUS @Z 5 aut. @Z 10 aut.`
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A21				`@1 2011`
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C01	`01`		`ENG`	@0 Detection of human Ag-specific T cells is limited by sensitivity and blood requirements. As dendritic cells (DCs) can potently stimulate T cells, we hypothesized that their induction in PBMCs in situ could link Ag processing and presentation to Ag-specific T-cell activation. To this end, unfractionated PBMCs (fresh or frozen) or whole blood were incubated for 48 hours with protein or peptide Ag together with different DC-activating agents to rapidly and sequentially induce, pulse, and mature DCs. DC activation was therefore lined up with Ag recognition by neighboring T cells, thus telescoping the sequential steps of T-cell activation. Efficient processing of protein Ags made prior knowledge of epitopes and HLA restrictions dispensable. While reducing stimulation time, manipulation and blood requirements, in situ DC induction specifically amplified Ag-specific T-cell responses (cytokine secretion, proliferation, CD137/CD154 up-regulation, and binding of peptide-HLA multimers). IL-1β, although released by DCs, was also secreted in an Ag-specific fashion, thus providing an indirect biomarker of T-cell responses. These accelerated cocultured DC (acDC) assays offered a sensitive means with which to evaluate T-cell responses to viral and melanoma Ag vaccination, and may therefore find application for immune monitoring in viral, tumor, autoimmune, and transplantation settings.
C02	`01`	`X`		`@0 002B19`
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C03	`02`	`X`	`SPA`	`@0 Respuesta inmune @5 03`
C03	`03`	`X`	`FRE`	`@0 Hématologie @5 05`
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C03	`03`	`X`	`SPA`	`@0 Hematología @5 05`
C03	`04`	`X`	`FRE`	`@0 Spécificité antigénique @4 CD @5 96`
C03	`04`	`X`	`ENG`	`@0 Antigen specific @4 CD @5 96`
N21				`@1 283`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Pascal:11-0412241

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">acDCs enhance human antigen-specific T-cell responses</title>
<author><name sortKey="Martinuzzi, Emanuela" sort="Martinuzzi, Emanuela" uniqKey="Martinuzzi E" first="Emanuela" last="Martinuzzi">Emanuela Martinuzzi</name>
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<author><name sortKey="Afonso, Georgia" sort="Afonso, Georgia" uniqKey="Afonso G" first="Georgia" last="Afonso">Georgia Afonso</name>
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<author><name sortKey="Gagnerault, Marie Claude" sort="Gagnerault, Marie Claude" uniqKey="Gagnerault M" first="Marie-Claude" last="Gagnerault">Marie-Claude Gagnerault</name>
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<author><name sortKey="Naselli, Gaetano" sort="Naselli, Gaetano" uniqKey="Naselli G" first="Gaetano" last="Naselli">Gaetano Naselli</name>
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<author><name sortKey="Mittag, Diana" sort="Mittag, Diana" uniqKey="Mittag D" first="Diana" last="Mittag">Diana Mittag</name>
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<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
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<author><name sortKey="Zitvogel, Laurence" sort="Zitvogel, Laurence" uniqKey="Zitvogel L" first="Laurence" last="Zitvogel">Laurence Zitvogel</name>
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<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre d'Investigation Clinique Biothérapie 507</s1>
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<country>France</country>
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<country>France</country>
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<sZ>10 aut.</sZ>
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<country>Australie</country>
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<author><name sortKey="Mallone, Roberto" sort="Mallone, Roberto" uniqKey="Mallone R" first="Roberto" last="Mallone">Roberto Mallone</name>
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<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
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<country>France</country>
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<series><title level="j" type="main">Blood</title>
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<front><div type="abstract" xml:lang="en">Detection of human Ag-specific T cells is limited by sensitivity and blood requirements. As dendritic cells (DCs) can potently stimulate T cells, we hypothesized that their induction in PBMCs in situ could link Ag processing and presentation to Ag-specific T-cell activation. To this end, unfractionated PBMCs (fresh or frozen) or whole blood were incubated for 48 hours with protein or peptide Ag together with different DC-activating agents to rapidly and sequentially induce, pulse, and mature DCs. DC activation was therefore lined up with Ag recognition by neighboring T cells, thus telescoping the sequential steps of T-cell activation. Efficient processing of protein Ags made prior knowledge of epitopes and HLA restrictions dispensable. While reducing stimulation time, manipulation and blood requirements, in situ DC induction specifically amplified Ag-specific T-cell responses (cytokine secretion, proliferation, CD137/CD154 up-regulation, and binding of peptide-HLA multimers). IL-1β, although released by DCs, was also secreted in an Ag-specific fashion, thus providing an indirect biomarker of T-cell responses. These accelerated cocultured DC (acDC) assays offered a sensitive means with which to evaluate T-cell responses to viral and melanoma Ag vaccination, and may therefore find application for immune monitoring in viral, tumor, autoimmune, and transplantation settings.</div>
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	Serveur d'exploration sur les relations entre la France et l'Australie
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acDCs enhance human antigen-specific T-cell responses

acDCs enhance human antigen-specific T-cell responses

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