AustralieFrV1, PascalFrancis, Curation, bibRecord, 003D06

Effects of post-mortem intervals on regional brain protein profiles in rats using SELDI-TOF-MS analysis

Identifieur interne : 003D06 ( PascalFrancis/Curation ); précédent : 003D05; suivant : 003D07

Effects of post-mortem intervals on regional brain protein profiles in rats using SELDI-TOF-MS analysis

Auteurs : Rita Machaalani [Australie, France] ; Evelyne Gozal [États-Unis] ; François Berger [France] ; Karen A. Waters [Australie] ; Maurice Dematteis [France]

Source :

Neurochemistry international [ 0197-0186 ] ; 2010.

RBID : Pascal:10-0487265

Descripteurs français

Pascal (Inist)
- Encéphale, Protéine, Protéomique, Rat, Animal.

English descriptors

KwdEn :
- Animal, Encephalon, Protein, Proteomics, Rat.

Abstract

Identification of disease-associated proteins is critical for elucidating CNS disease mechanisms and elaborating novel treatment strategies. It requires post-mortem tissue analysis which can be significantly affected by the collection process, post-mortem intervals (PMIs), and storage conditions. To assess the effect of time and storage conditions on brain protein stability, SELDI-TOF-MS protein profiles were assessed in rat frontal cortex, caudate-putamen, hippocampus and medulla samples collected after various PMIs (0, 6, 12, 24, 48, and 72 h) at 4 °C or at room temperature (RT) storage. Regions of interest were isolated from cryosections (tissue apposition, TA), or micropunched from cryosections apposed on filter paper (paper apposition, PA), and applied onto an NP20 ProteinChip® array. Protein alterations, while greater at RT than at 4 °C, were detected at 6 h then differentially evolved in the various brain regions, with greater alterations in the caudate-putamen (60%) and the cortex (48%). Overall, our sensitive analytical method allowed unveiling of different patterns of protein susceptibility to PMI and to storage temperature in the various brain regions. Some protein peaks were altered in all brain regions and may potentially serve as markers of the PMI status of the brain, or for reference values when studying new proteins. Changes in disease-related proteins within post-mortem samples can be greatly affected by PMI and storage conditions, particularly when studying fragile and/or low abundant protein/peptides in tissues sampled from the caudate-putamen and neocortex.

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Identification of disease-associated proteins is critical for elucidating CNS disease mechanisms and elaborating novel treatment strategies. It requires post-mortem tissue analysis which can be significantly affected by the collection process, post-mortem intervals (PMIs), and storage conditions. To assess the effect of time and storage conditions on brain protein stability, SELDI-TOF-MS protein profiles were assessed in rat frontal cortex, caudate-putamen, hippocampus and medulla samples collected after various PMIs (0, 6, 12, 24, 48, and 72 h) at 4 °C or at room temperature (RT) storage. Regions of interest were isolated from cryosections (tissue apposition, TA), or micropunched from cryosections apposed on filter paper (paper apposition, PA), and applied onto an NP20 ProteinChip® array. Protein alterations, while greater at RT than at 4 °C, were detected at 6 h then differentially evolved in the various brain regions, with greater alterations in the caudate-putamen (60%) and the cortex (48%). Overall, our sensitive analytical method allowed unveiling of different patterns of protein susceptibility to PMI and to storage temperature in the various brain regions. Some protein peaks were altered in all brain regions and may potentially serve as markers of the PMI status of the brain, or for reference values when studying new proteins. Changes in disease-related proteins within post-mortem samples can be greatly affected by PMI and storage conditions, particularly when studying fragile and/or low abundant protein/peptides in tissues sampled from the caudate-putamen and neocortex.</div>
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<s1>© 2010 INIST-CNRS. All rights reserved.</s1>
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<fA45><s0>1/4 p.</s0>
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<fA47 i1="01" i2="1"><s0>10-0487265</s0>
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<fA64 i1="01" i2="1"><s0>Neurochemistry international</s0>
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<fC01 i1="01" l="ENG"><s0>Identification of disease-associated proteins is critical for elucidating CNS disease mechanisms and elaborating novel treatment strategies. It requires post-mortem tissue analysis which can be significantly affected by the collection process, post-mortem intervals (PMIs), and storage conditions. To assess the effect of time and storage conditions on brain protein stability, SELDI-TOF-MS protein profiles were assessed in rat frontal cortex, caudate-putamen, hippocampus and medulla samples collected after various PMIs (0, 6, 12, 24, 48, and 72 h) at 4 °C or at room temperature (RT) storage. Regions of interest were isolated from cryosections (tissue apposition, TA), or micropunched from cryosections apposed on filter paper (paper apposition, PA), and applied onto an NP20 ProteinChip® array. Protein alterations, while greater at RT than at 4 °C, were detected at 6 h then differentially evolved in the various brain regions, with greater alterations in the caudate-putamen (60%) and the cortex (48%). Overall, our sensitive analytical method allowed unveiling of different patterns of protein susceptibility to PMI and to storage temperature in the various brain regions. Some protein peaks were altered in all brain regions and may potentially serve as markers of the PMI status of the brain, or for reference values when studying new proteins. Changes in disease-related proteins within post-mortem samples can be greatly affected by PMI and storage conditions, particularly when studying fragile and/or low abundant protein/peptides in tissues sampled from the caudate-putamen and neocortex.</s0>
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<s5>69</s5>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<fC07 i1="03" i2="X" l="ENG"><s0>Mammalia</s0>
<s2>NS</s2>
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<s2>NS</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
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<fN21><s1>326</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
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<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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Effects of post-mortem intervals on regional brain protein profiles in rats using SELDI-TOF-MS analysis

Effects of post-mortem intervals on regional brain protein profiles in rats using SELDI-TOF-MS analysis

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