Serveur d'exploration sur les relations entre la France et l'Australie

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Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species

Identifieur interne : 002A43 ( PascalFrancis/Curation ); précédent : 002A42; suivant : 002A44

Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species

Auteurs : S. Epis [Italie] ; D. Sassera [Italie] ; T. Beninati [Australie] ; N. Lo [Australie] ; L. Beati [États-Unis] ; J. Piesman [États-Unis] ; L. Rinaldi [Italie] ; K. D. Mccoy [France] ; A. Torina [Italie] ; L. Sacchi [Italie] ; E. Clementi [Italie] ; M. Genchi [Italie] ; S. Magnino [Italie] ; C. Bandi [Italie]

Source :

RBID : Pascal:08-0237571

Descripteurs français

English descriptors

Abstract

The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts.
pA  
A01 01  1    @0 0031-1820
A02 01      @0 PARAAE
A03   1    @0 Parasitology : (Lond., Print)
A05       @2 135
A06       @3 p. 4
A08 01  1  ENG  @1 Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species
A11 01  1    @1 EPIS (S.)
A11 02  1    @1 SASSERA (D.)
A11 03  1    @1 BENINATI (T.)
A11 04  1    @1 LO (N.)
A11 05  1    @1 BEATI (L.)
A11 06  1    @1 PIESMAN (J.)
A11 07  1    @1 RINALDI (L.)
A11 08  1    @1 MCCOY (K. D.)
A11 09  1    @1 TORINA (A.)
A11 10  1    @1 SACCHI (L.)
A11 11  1    @1 CLEMENTI (E.)
A11 12  1    @1 GENCHI (M.)
A11 13  1    @1 MAGNINO (S.)
A11 14  1    @1 BANDI (C.)
A14 01      @1 Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano @2 Milano @3 ITA @Z 1 aut. @Z 2 aut. @Z 14 aut.
A14 02      @1 School of Biological Sciences, The University of Sydney @2 New South Wales @3 AUS @Z 3 aut. @Z 4 aut.
A14 03      @1 Institute of Arthropodology and Parasitology, Georgia Southern University @2 Statesboro, GA @3 USA @Z 5 aut.
A14 04      @1 Division of Vector Borne Infectious Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention @2 Fort Collins, CO @3 USA @Z 6 aut.
A14 05      @1 Dipartimento di Patologia e Sanità Animale, Universita degli Studi di Napoli Federico II @2 Napoli @3 ITA @Z 7 aut.
A14 06      @1 Génétique et Evolution des Maladies Infectieuses, UMR CNRS-IRD 2724, IRD @2 Montpellier @3 FRA @Z 8 aut.
A14 07      @1 Istituto Zooprofilattico Sperimentale della Sicilia @2 Palermo @3 ITA @Z 9 aut.
A14 08      @1 Dipartimento di Biologia Animale, Università di Pavia, Piazza Botta 9 @2 Pavia @3 ITA @Z 10 aut. @Z 11 aut. @Z 12 aut.
A14 09      @1 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Sezione di Pavia @3 ITA @Z 13 aut.
A20       @1 485-494
A21       @1 2008
A23 01      @0 ENG
A43 01      @1 INIST @2 3187 @5 354000172651590080
A44       @0 0000 @1 © 2008 INIST-CNRS. All rights reserved.
A45       @0 3/4 p.
A47 01  1    @0 08-0237571
A60       @1 P
A61       @0 A
A64 01  1    @0 Parasitology : (London. Print)
A66 01      @0 GBR
C01 01    ENG  @0 The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts.
C02 01  X    @0 002A12A01
C02 02  X    @0 002A37A
C03 01  X  FRE  @0 Ectoparasite @5 01
C03 01  X  ENG  @0 Ectoparasite @5 01
C03 01  X  SPA  @0 Ectoparásito @5 01
C03 02  X  FRE  @0 Mitochondrie @5 02
C03 02  X  ENG  @0 Mitochondria @5 02
C03 02  X  SPA  @0 Mitocondria @5 02
C03 03  X  FRE  @0 Réaction chaîne polymérase @5 03
C03 03  X  ENG  @0 Polymerase chain reaction @5 03
C03 03  X  SPA  @0 Reacción cadena polimerasa @5 03
C03 04  X  FRE  @0 Phylogenèse @5 04
C03 04  X  ENG  @0 Phylogeny @5 04
C03 04  X  SPA  @0 Filogénesis @5 04
C03 05  X  FRE  @0 Parasite @5 05
C03 05  X  ENG  @0 Parasite @5 05
C03 05  X  SPA  @0 Parásito @5 05
C03 06  X  FRE  @0 Systématique @5 06
C03 06  X  ENG  @0 Taxonomy @5 06
C03 06  X  SPA  @0 Sistemática @5 06
C03 07  X  FRE  @0 Ixodidae @2 NS @5 55
C03 07  X  ENG  @0 Ixodidae @2 NS @5 55
C03 07  X  SPA  @0 Ixodidae @2 NS @5 55
C03 08  X  FRE  @0 Ixodes ricinus @2 NS @5 56
C03 08  X  ENG  @0 Ixodes ricinus @2 NS @5 56
C03 08  X  SPA  @0 Ixodes ricinus @2 NS @5 56
C07 01  X  FRE  @0 Ixodida @2 NS
C07 01  X  ENG  @0 Ixodida @2 NS
C07 01  X  SPA  @0 Ixodida @2 NS
C07 02  X  FRE  @0 Parasitiformes @2 NS
C07 02  X  ENG  @0 Parasitiformes @2 NS
C07 02  X  SPA  @0 Parasitiformes @2 NS
C07 03  X  FRE  @0 Acari @2 NS
C07 03  X  ENG  @0 Acari @2 NS
C07 03  X  SPA  @0 Acari @2 NS
C07 04  X  FRE  @0 Arachnida @2 NS
C07 04  X  ENG  @0 Arachnida @2 NS
C07 04  X  SPA  @0 Arachnida @2 NS
C07 05  X  FRE  @0 Arthropoda @2 NS
C07 05  X  ENG  @0 Arthropoda @2 NS
C07 05  X  SPA  @0 Arthropoda @2 NS
C07 06  X  FRE  @0 Invertebrata @2 NS
C07 06  X  ENG  @0 Invertebrata @2 NS
C07 06  X  SPA  @0 Invertebrata @2 NS
N21       @1 154
N44 01      @1 OTO
N82       @1 OTO

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Pascal:08-0237571

Le document en format XML

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<author>
<name sortKey="Magnino, S" sort="Magnino, S" uniqKey="Magnino S" first="S." last="Magnino">S. Magnino</name>
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<s1>Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Sezione di Pavia</s1>
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<name sortKey="Bandi, C" sort="Bandi, C" uniqKey="Bandi C" first="C." last="Bandi">C. Bandi</name>
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<s1>Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano</s1>
<s2>Milano</s2>
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<country>Italie</country>
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<title level="j" type="main">Parasitology : (London. Print)</title>
<title level="j" type="abbreviated">Parasitology : (Lond., Print)</title>
<idno type="ISSN">0031-1820</idno>
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<date when="2008">2008</date>
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<title level="j" type="main">Parasitology : (London. Print)</title>
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<term>Ectoparasite</term>
<term>Ixodes ricinus</term>
<term>Ixodidae</term>
<term>Mitochondria</term>
<term>Parasite</term>
<term>Phylogeny</term>
<term>Polymerase chain reaction</term>
<term>Taxonomy</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr">
<term>Ectoparasite</term>
<term>Mitochondrie</term>
<term>Réaction chaîne polymérase</term>
<term>Phylogenèse</term>
<term>Parasite</term>
<term>Systématique</term>
<term>Ixodidae</term>
<term>Ixodes ricinus</term>
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<div type="abstract" xml:lang="en">The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts.</div>
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<sZ>11 aut.</sZ>
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<s0>The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts.</s0>
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