AustralieFrV1, PascalFrancis, Corpus, bibRecord, 006303

Identification and characterization of plant transporters using heterologous expression systems

Identifieur interne : 006303 ( PascalFrancis/Corpus ); précédent : 006302; suivant : 006304

Identification and characterization of plant transporters using heterologous expression systems

Auteurs : I. Dreyer ; C. Horeau ; G. Lemaillet ; S. Zimmermann ; D. R. Bush ; A. Rodriguez-Navarro ; D. P. Schachtman ; E. P. Spalding ; H. Sentenac ; R. F. Gaber

Source :

Journal of Experimental Botany [ 0022-0957 ] ; 1999.

RBID : Pascal:99-0374850

Descripteurs français

Pascal (Inist)
- Transport membranaire, Protéine transport, Caractérisation, Expression génique, Système hétérologue, Mutation, Relation structure fonction, Végétal.

English descriptors

KwdEn :
- Carrier protein, Characterization, Gene expression, Heterologous system, Membrane transport, Mutation, Structure function relationship, Vegetals.

Abstract

In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0022-0957`
A02	`01`			`@0 JEBOA6`
A03		`1`		`@0 J. Exp. Bot.`
A05				`@2 50`
A06				`@3 NS`
A08	`01`	`1`	`ENG`	`@1 Identification and characterization of plant transporters using heterologous expression systems`
A09	`01`	`1`	`ENG`	`@1 Membrane transport: mechanisms, modulation and manipulation`
A11	`01`	`1`		`@1 DREYER (I.)`
A11	`02`	`1`		`@1 HOREAU (C.)`
A11	`03`	`1`		`@1 LEMAILLET (G.)`
A11	`04`	`1`		`@1 ZIMMERMANN (S.)`
A11	`05`	`1`		`@1 BUSH (D. R.)`
A11	`06`	`1`		`@1 RODRIGUEZ-NAVARRO (A.)`
A11	`07`	`1`		`@1 SCHACHTMAN (D. P.)`
A11	`08`	`1`		`@1 SPALDING (E. P.)`
A11	`09`	`1`		`@1 SENTENAC (H.)`
A11	`10`	`1`		`@1 GABER (R. F.)`
A12	`01`	`1`		`@1 HALL (John) @9 ed.`
A12	`02`	`1`		`@1 BLATT (Michael) @9 ed.`
A12	`03`	`1`		`@1 LEIGH (Roger) @9 ed.`
A14	`01`			`@1 Biochimie et Physiologie Moléculaire des Plantes, UMII/Agro-M/INRA/CNR URA 2133, Place Viala @2 34060 Montpellier @3 FRA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 9 aut.`
A14	`02`			`@1 Max-Planck-lnstitut für Molekulare Pflanzenphysiologie @2 14476 Golm/Potsdam @3 DEU @Z 4 aut.`
A14	`03`			`@1 Department of Plant Biology and Photosynthesis Research Unit, US Department of Agriculture-Agricultural Research Service, University of Illinois at Urbana-Champain @2 Urbana, IL 61801 @3 USA @Z 5 aut.`
A14	`04`			`@1 Departamento de Biotecnologia, Escuela Técnica Superior de Ingenieros Agrónomos @2 28040 Madrid @3 ESP @Z 6 aut.`
A14	`05`			`@1 Department of Botany, University of Adelaide @2 Adelaide, SA 5005 @3 AUS @Z 7 aut.`
A14	`06`			`@1 Department of Botany and Program in Cellular and Molecular Biology, University of Wisconsin @2 Madison, WI 53706 @3 USA @Z 8 aut.`
A14	`07`			`@1 Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University @2 Evanston, IL 60208 @3 USA @Z 10 aut.`
A20				`@1 1073-1087`
A21				`@1 1999`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 6923 @5 354000085387880150`
A44				`@0 0000 @1 © 1999 INIST-CNRS. All rights reserved.`
A45				`@0 4 p.1/4`
A47	`01`	`1`		`@0 99-0374850`
A60				`@1 P @2 C`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of Experimental Botany`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.
C02	`01`	`X`		`@0 002A10B03`
C03	`01`	`X`	`FRE`	`@0 Transport membranaire @5 01`
C03	`01`	`X`	`ENG`	`@0 Membrane transport @5 01`
C03	`01`	`X`	`SPA`	`@0 Transporte membranal @5 01`
C03	`02`	`X`	`FRE`	`@0 Protéine transport @5 02`
C03	`02`	`X`	`ENG`	`@0 Carrier protein @5 02`
C03	`02`	`X`	`SPA`	`@0 Proteína transportador @5 02`
C03	`03`	`X`	`FRE`	`@0 Caractérisation @5 03`
C03	`03`	`X`	`ENG`	`@0 Characterization @5 03`
C03	`03`	`X`	`SPA`	`@0 Caracterización @5 03`
C03	`04`	`X`	`FRE`	`@0 Expression génique @5 04`
C03	`04`	`X`	`ENG`	`@0 Gene expression @5 04`
C03	`04`	`X`	`SPA`	`@0 Expresión genética @5 04`
C03	`05`	`X`	`FRE`	`@0 Système hétérologue @5 05`
C03	`05`	`X`	`ENG`	`@0 Heterologous system @5 05`
C03	`05`	`X`	`SPA`	`@0 Sistema heterólogo @5 05`
C03	`06`	`X`	`FRE`	`@0 Mutation @5 06`
C03	`06`	`X`	`ENG`	`@0 Mutation @5 06`
C03	`06`	`X`	`SPA`	`@0 Mutación @5 06`
C03	`07`	`X`	`FRE`	`@0 Relation structure fonction @5 07`
C03	`07`	`X`	`ENG`	`@0 Structure function relationship @5 07`
C03	`07`	`X`	`SPA`	`@0 Relación estructura función @5 07`
C03	`08`	`X`	`FRE`	`@0 Végétal @5 08`
C03	`08`	`X`	`ENG`	`@0 Vegetals @5 08`
C03	`08`	`X`	`SPA`	`@0 Vegetal @5 08`
N21				`@1 242`

A30	`01`	`1`	`ENG`	`@1 International Workshop on Plant Membrane Biology @2 11 @3 Cambridge GBR @4 1998-08-09`

Format Inist (serveur)

NO :	PASCAL 99-0374850 INIST
ET :	Identification and characterization of plant transporters using heterologous expression systems
AU :	DREYER (I.); HOREAU (C.); LEMAILLET (G.); ZIMMERMANN (S.); BUSH (D. R.); RODRIGUEZ-NAVARRO (A.); SCHACHTMAN (D. P.); SPALDING (E. P.); SENTENAC (H.); GABER (R. F.); HALL (John); BLATT (Michael); LEIGH (Roger)
AF :	Biochimie et Physiologie Moléculaire des Plantes, UMII/Agro-M/INRA/CNR URA 2133, Place Viala/34060 Montpellier/France (1 aut., 2 aut., 3 aut., 9 aut.); Max-Planck-lnstitut für Molekulare Pflanzenphysiologie/14476 Golm/Potsdam/Allemagne (4 aut.); Department of Plant Biology and Photosynthesis Research Unit, US Department of Agriculture-Agricultural Research Service, University of Illinois at Urbana-Champain/Urbana, IL 61801/Etats-Unis (5 aut.); Departamento de Biotecnologia, Escuela Técnica Superior de Ingenieros Agrónomos/28040 Madrid/Espagne (6 aut.); Department of Botany, University of Adelaide/Adelaide, SA 5005/Australie (7 aut.); Department of Botany and Program in Cellular and Molecular Biology, University of Wisconsin/Madison, WI 53706/Etats-Unis (8 aut.); Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University/Evanston, IL 60208/Etats-Unis (10 aut.)
DT :	Publication en série; Congrès; Niveau analytique
SO :	Journal of Experimental Botany; ISSN 0022-0957; Coden JEBOA6; Royaume-Uni; Da. 1999; Vol. 50; No. NS; Pp. 1073-1087; Bibl. 4 p.1/4
LA :	Anglais
EA :	In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.
CC :	002A10B03
FD :	Transport membranaire; Protéine transport; Caractérisation; Expression génique; Système hétérologue; Mutation; Relation structure fonction; Végétal
ED :	Membrane transport; Carrier protein; Characterization; Gene expression; Heterologous system; Mutation; Structure function relationship; Vegetals
SD :	Transporte membranal; Proteína transportador; Caracterización; Expresión genética; Sistema heterólogo; Mutación; Relación estructura función; Vegetal
LO :	INIST-6923.354000085387880150
ID :	99-0374850

Links to Exploration step

Pascal:99-0374850

Le document en format XML

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<author><name sortKey="Bush, D R" sort="Bush, D R" uniqKey="Bush D" first="D. R." last="Bush">D. R. Bush</name>
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<s2>28040 Madrid</s2>
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<author><name sortKey="Schachtman, D P" sort="Schachtman, D P" uniqKey="Schachtman D" first="D. P." last="Schachtman">D. P. Schachtman</name>
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<series><title level="j" type="main">Journal of Experimental Botany</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carrier protein</term>
<term>Characterization</term>
<term>Gene expression</term>
<term>Heterologous system</term>
<term>Membrane transport</term>
<term>Mutation</term>
<term>Structure function relationship</term>
<term>Vegetals</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Transport membranaire</term>
<term>Protéine transport</term>
<term>Caractérisation</term>
<term>Expression génique</term>
<term>Système hétérologue</term>
<term>Mutation</term>
<term>Relation structure fonction</term>
<term>Végétal</term>
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<front><div type="abstract" xml:lang="en">In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>Identification and characterization of plant transporters using heterologous expression systems</s1>
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<fA09 i1="01" i2="1" l="ENG"><s1>Membrane transport: mechanisms, modulation and manipulation</s1>
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<fA11 i1="01" i2="1"><s1>DREYER (I.)</s1>
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<fA11 i1="02" i2="1"><s1>HOREAU (C.)</s1>
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<fA11 i1="07" i2="1"><s1>SCHACHTMAN (D. P.)</s1>
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<fA11 i1="08" i2="1"><s1>SPALDING (E. P.)</s1>
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<s9>ed.</s9>
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<fA12 i1="02" i2="1"><s1>BLATT (Michael)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="03" i2="1"><s1>LEIGH (Roger)</s1>
<s9>ed.</s9>
</fA12>
<fA14 i1="01"><s1>Biochimie et Physiologie Moléculaire des Plantes, UMII/Agro-M/INRA/CNR URA 2133, Place Viala</s1>
<s2>34060 Montpellier</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Max-Planck-lnstitut für Molekulare Pflanzenphysiologie</s1>
<s2>14476 Golm/Potsdam</s2>
<s3>DEU</s3>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Plant Biology and Photosynthesis Research Unit, US Department of Agriculture-Agricultural Research Service, University of Illinois at Urbana-Champain</s1>
<s2>Urbana, IL 61801</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Departamento de Biotecnologia, Escuela Técnica Superior de Ingenieros Agrónomos</s1>
<s2>28040 Madrid</s2>
<s3>ESP</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Botany, University of Adelaide</s1>
<s2>Adelaide, SA 5005</s2>
<s3>AUS</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Department of Botany and Program in Cellular and Molecular Biology, University of Wisconsin</s1>
<s2>Madison, WI 53706</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="07"><s1>Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University</s1>
<s2>Evanston, IL 60208</s2>
<s3>USA</s3>
<sZ>10 aut.</sZ>
</fA14>
<fA20><s1>1073-1087</s1>
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<fA21><s1>1999</s1>
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<fA64 i1="01" i2="1"><s0>Journal of Experimental Botany</s0>
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<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.</s0>
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<s5>02</s5>
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<fC03 i1="03" i2="X" l="FRE"><s0>Caractérisation</s0>
<s5>03</s5>
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<s5>06</s5>
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<fC03 i1="07" i2="X" l="FRE"><s0>Relation structure fonction</s0>
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<s5>08</s5>
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<fC03 i1="08" i2="X" l="ENG"><s0>Vegetals</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Vegetal</s0>
<s5>08</s5>
</fC03>
<fN21><s1>242</s1>
</fN21>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>International Workshop on Plant Membrane Biology</s1>
<s2>11</s2>
<s3>Cambridge GBR</s3>
<s4>1998-08-09</s4>
</fA30>
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<server><NO>PASCAL 99-0374850 INIST</NO>
<ET>Identification and characterization of plant transporters using heterologous expression systems</ET>
<AU>DREYER (I.); HOREAU (C.); LEMAILLET (G.); ZIMMERMANN (S.); BUSH (D. R.); RODRIGUEZ-NAVARRO (A.); SCHACHTMAN (D. P.); SPALDING (E. P.); SENTENAC (H.); GABER (R. F.); HALL (John); BLATT (Michael); LEIGH (Roger)</AU>
<AF>Biochimie et Physiologie Moléculaire des Plantes, UMII/Agro-M/INRA/CNR URA 2133, Place Viala/34060 Montpellier/France (1 aut., 2 aut., 3 aut., 9 aut.); Max-Planck-lnstitut für Molekulare Pflanzenphysiologie/14476 Golm/Potsdam/Allemagne (4 aut.); Department of Plant Biology and Photosynthesis Research Unit, US Department of Agriculture-Agricultural Research Service, University of Illinois at Urbana-Champain/Urbana, IL 61801/Etats-Unis (5 aut.); Departamento de Biotecnologia, Escuela Técnica Superior de Ingenieros Agrónomos/28040 Madrid/Espagne (6 aut.); Department of Botany, University of Adelaide/Adelaide, SA 5005/Australie (7 aut.); Department of Botany and Program in Cellular and Molecular Biology, University of Wisconsin/Madison, WI 53706/Etats-Unis (8 aut.); Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University/Evanston, IL 60208/Etats-Unis (10 aut.)</AF>
<DT>Publication en série; Congrès; Niveau analytique</DT>
<SO>Journal of Experimental Botany; ISSN 0022-0957; Coden JEBOA6; Royaume-Uni; Da. 1999; Vol. 50; No. NS; Pp. 1073-1087; Bibl. 4 p.1/4</SO>
<LA>Anglais</LA>
<EA>In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.</EA>
<CC>002A10B03</CC>
<FD>Transport membranaire; Protéine transport; Caractérisation; Expression génique; Système hétérologue; Mutation; Relation structure fonction; Végétal</FD>
<ED>Membrane transport; Carrier protein; Characterization; Gene expression; Heterologous system; Mutation; Structure function relationship; Vegetals</ED>
<SD>Transporte membranal; Proteína transportador; Caracterización; Expresión genética; Sistema heterólogo; Mutación; Relación estructura función; Vegetal</SD>
<LO>INIST-6923.354000085387880150</LO>
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Identification and characterization of plant transporters using heterologous expression systems

Identification and characterization of plant transporters using heterologous expression systems

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