Analysis of cyanophage diversity and population structure in a south-north transect of the Atlantic ocean
Identifieur interne : 006145 ( PascalFrancis/Checkpoint ); précédent : 006144; suivant : 006146Analysis of cyanophage diversity and population structure in a south-north transect of the Atlantic ocean
Auteurs : W. H. Wilson [Royaume-Uni] ; N. J. Fuller [Royaume-Uni] ; I. R. Joint [Royaume-Uni] ; N. H. Mann [Royaume-Uni]Source :
- Bulletin de l'Institut océanographique : (Monaco) [ 0304-5722 ] ; 1999.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Océan Atlantique.
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Abstract
Cyanophages (viruses which infect cyanobacteria) are abundant in the marine environment and are thought to be a significant factor in determining the dynamics of Synechococcus spp. populations. In an effort to use molecular techniques to characterise cyanophage populations, we designed cyanophage-specific (CPS) PCR primers based on a gene found in three genetically distinct marine cyanophages (Fuller et al., 1998). CPS primers were used to amplify cyanophage DNA extracted from viral communities concentrated from seawater samples obtained during a cruise transect between the Falkland Islands, in the south Atlantic ocean, to the UK. Following phylogenetic analysis of cloned and sequenced PCR products, it was revealed that genetic diversity of marine cyanophage clones within a single water sample was as great as clones and cyanophage isolates collected between different oceans. Denaturing gradient gel electrophoresis (DGGE) analysis confirmed this high diversity. DGGE analysis also revealed changes in cyanophage population structure in surface seawater over the south-north transect and throughout depth profiles in the water column. Maximum Synechococcus spp. concentrations, in a stratified water column, correlated with maximum cyanophage diversity.
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<front><div type="abstract" xml:lang="en">Cyanophages (viruses which infect cyanobacteria) are abundant in the marine environment and are thought to be a significant factor in determining the dynamics of Synechococcus spp. populations. In an effort to use molecular techniques to characterise cyanophage populations, we designed cyanophage-specific (CPS) PCR primers based on a gene found in three genetically distinct marine cyanophages (Fuller et al., 1998). CPS primers were used to amplify cyanophage DNA extracted from viral communities concentrated from seawater samples obtained during a cruise transect between the Falkland Islands, in the south Atlantic ocean, to the UK. Following phylogenetic analysis of cloned and sequenced PCR products, it was revealed that genetic diversity of marine cyanophage clones within a single water sample was as great as clones and cyanophage isolates collected between different oceans. Denaturing gradient gel electrophoresis (DGGE) analysis confirmed this high diversity. DGGE analysis also revealed changes in cyanophage population structure in surface seawater over the south-north transect and throughout depth profiles in the water column. Maximum Synechococcus spp. concentrations, in a stratified water column, correlated with maximum cyanophage diversity.</div>
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