AustralieFrV1, PascalFrancis, Checkpoint, bibRecord, 006145

Analysis of cyanophage diversity and population structure in a south-north transect of the Atlantic ocean

Identifieur interne : 006145 ( PascalFrancis/Checkpoint ); précédent : 006144; suivant : 006146

Analysis of cyanophage diversity and population structure in a south-north transect of the Atlantic ocean

Auteurs : W. H. Wilson [Royaume-Uni] ; N. J. Fuller [Royaume-Uni] ; I. R. Joint [Royaume-Uni] ; N. H. Mann [Royaume-Uni]

Source :

Bulletin de l'Institut océanographique : (Monaco) [ 0304-5722 ] ; 1999.

RBID : Pascal:00-0052631

Descripteurs français

Pascal (Inist)
- Cyanobacteria, Cyanophage, Structure population, Répartition verticale, Diversité biologique, Diversité génétique, Réaction chaîne polymérase, Relation hôte virus, Dynamique population, Régulation naturelle population, Océan Atlantique.
Wicri :
- topic : Océan Atlantique.

English descriptors

KwdEn :
- Atlantic Ocean, Biodiversity, Cyanobacteria, Cyanophage, Genetic diversity, Host virus relation, Polymerase chain reaction, Population dynamics, Population regulation, Population structure, Vertical distribution.

Abstract

Cyanophages (viruses which infect cyanobacteria) are abundant in the marine environment and are thought to be a significant factor in determining the dynamics of Synechococcus spp. populations. In an effort to use molecular techniques to characterise cyanophage populations, we designed cyanophage-specific (CPS) PCR primers based on a gene found in three genetically distinct marine cyanophages (Fuller et al., 1998). CPS primers were used to amplify cyanophage DNA extracted from viral communities concentrated from seawater samples obtained during a cruise transect between the Falkland Islands, in the south Atlantic ocean, to the UK. Following phylogenetic analysis of cloned and sequenced PCR products, it was revealed that genetic diversity of marine cyanophage clones within a single water sample was as great as clones and cyanophage isolates collected between different oceans. Denaturing gradient gel electrophoresis (DGGE) analysis confirmed this high diversity. DGGE analysis also revealed changes in cyanophage population structure in surface seawater over the south-north transect and throughout depth profiles in the water column. Maximum Synechococcus spp. concentrations, in a stratified water column, correlated with maximum cyanophage diversity.

Affiliations:

Royaume-Uni

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Cyanophages (viruses which infect cyanobacteria) are abundant in the marine environment and are thought to be a significant factor in determining the dynamics of Synechococcus spp. populations. In an effort to use molecular techniques to characterise cyanophage populations, we designed cyanophage-specific (CPS) PCR primers based on a gene found in three genetically distinct marine cyanophages (Fuller et al., 1998). CPS primers were used to amplify cyanophage DNA extracted from viral communities concentrated from seawater samples obtained during a cruise transect between the Falkland Islands, in the south Atlantic ocean, to the UK. Following phylogenetic analysis of cloned and sequenced PCR products, it was revealed that genetic diversity of marine cyanophage clones within a single water sample was as great as clones and cyanophage isolates collected between different oceans. Denaturing gradient gel electrophoresis (DGGE) analysis confirmed this high diversity. DGGE analysis also revealed changes in cyanophage population structure in surface seawater over the south-north transect and throughout depth profiles in the water column. Maximum Synechococcus spp. concentrations, in a stratified water column, correlated with maximum cyanophage diversity.</div>
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Analysis of cyanophage diversity and population structure in a south-north transect of the Atlantic ocean

Analysis of cyanophage diversity and population structure in a south-north transect of the Atlantic ocean

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