Coculturing with endothelial cells promotes in vitro maturation and electrical coupling of human embryonic stem cell-derived cardiomyocytes.
Identifieur interne : 004284 ( Ncbi/Merge ); précédent : 004283; suivant : 004285Coculturing with endothelial cells promotes in vitro maturation and electrical coupling of human embryonic stem cell-derived cardiomyocytes.
Auteurs : Jennifer Pasquier [États-Unis] ; Renuka Gupta [Qatar] ; Damien Rioult [France] ; Jessica Hoarau-Véchot [Qatar] ; Raphael Courjaret [Qatar] ; Khaled Machaca [Qatar] ; Jassim Al Suwaidi [Qatar] ; Edouard G. Stanley [Australie] ; Shahin Rafii [États-Unis] ; David A. Elliott [Australie] ; Charbel Abi Khalil [États-Unis] ; Arash Rafii [États-Unis]Source :
- The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation [ 1557-3117 ] ; 2017.
Abstract
Pluripotent human embryonic stem cells (hESC) are a promising source of repopulating cardiomyocytes. We hypothesized that we could improve maturation of cardiomyocytes and facilitate electrical interconnections by creating a model that more closely resembles heart tissue; that is, containing both endothelial cells (ECs) and cardiomyocytes.
DOI: 10.1016/j.healun.2017.01.001
PubMed: 28169114
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Coculturing with endothelial cells promotes in vitro maturation and electrical coupling of human embryonic stem cell-derived cardiomyocytes.</title><author><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name><affiliation wicri:level="2"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Gupta, Renuka" sort="Gupta, Renuka" uniqKey="Gupta R" first="Renuka" last="Gupta">Renuka Gupta</name><affiliation wicri:level="1"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name><affiliation wicri:level="3"><nlm:affiliation>UMR_I 02 INERIS-URCA-ULH Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims, Reims, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR_I 02 INERIS-URCA-ULH Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims, Reims</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName></affiliation></author><author><name sortKey="Hoarau Vechot, Jessica" sort="Hoarau Vechot, Jessica" uniqKey="Hoarau Vechot J" first="Jessica" last="Hoarau-Véchot">Jessica Hoarau-Véchot</name><affiliation wicri:level="1"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Courjaret, Raphael" sort="Courjaret, Raphael" uniqKey="Courjaret R" first="Raphael" last="Courjaret">Raphael Courjaret</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Machaca, Khaled" sort="Machaca, Khaled" uniqKey="Machaca K" first="Khaled" last="Machaca">Khaled Machaca</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Al Suwaidi, Jassim" sort="Al Suwaidi, Jassim" uniqKey="Al Suwaidi J" first="Jassim" last="Al Suwaidi">Jassim Al Suwaidi</name><affiliation wicri:level="1"><nlm:affiliation>Heart Hospital, Hamad Medical Corporation, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Heart Hospital, Hamad Medical Corporation, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Rafii, Shahin" sort="Rafii, Shahin" uniqKey="Rafii S" first="Shahin" last="Rafii">Shahin Rafii</name><affiliation wicri:level="2"><nlm:affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Medicine, Weill Cornell Medicine, New York</wicri:cityArea></affiliation></author><author><name sortKey="Elliott, David A" sort="Elliott, David A" uniqKey="Elliott D" first="David A" last="Elliott">David A. Elliott</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Abi Khalil, Charbel" sort="Abi Khalil, Charbel" uniqKey="Abi Khalil C" first="Charbel" last="Abi Khalil">Charbel Abi Khalil</name><affiliation wicri:level="2"><nlm:affiliation>Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar; Heart Hospital, Hamad Medical Corporation, Doha, Qatar; Department of Medicine, Weill Cornell Medicine, New York, New York, USA. Electronic address: cha2022@med.cornell.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar; Heart Hospital, Hamad Medical Corporation, Doha, Qatar; Department of Medicine, Weill Cornell Medicine, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Rafii, Arash" sort="Rafii, Arash" uniqKey="Rafii A" first="Arash" last="Rafii">Arash Rafii</name><affiliation wicri:level="2"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA. Electronic address: jat2021@med.cornell.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28169114</idno><idno type="pmid">28169114</idno><idno type="doi">10.1016/j.healun.2017.01.001</idno><idno type="wicri:Area/PubMed/Corpus">000A74</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A74</idno><idno type="wicri:Area/PubMed/Curation">000A71</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A71</idno><idno type="wicri:Area/PubMed/Checkpoint">000A71</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000A71</idno><idno type="wicri:Area/Ncbi/Merge">004284</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Coculturing with endothelial cells promotes in vitro maturation and electrical coupling of human embryonic stem cell-derived cardiomyocytes.</title><author><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name><affiliation wicri:level="2"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Gupta, Renuka" sort="Gupta, Renuka" uniqKey="Gupta R" first="Renuka" last="Gupta">Renuka Gupta</name><affiliation wicri:level="1"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name><affiliation wicri:level="3"><nlm:affiliation>UMR_I 02 INERIS-URCA-ULH Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims, Reims, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR_I 02 INERIS-URCA-ULH Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims, Reims</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName></affiliation></author><author><name sortKey="Hoarau Vechot, Jessica" sort="Hoarau Vechot, Jessica" uniqKey="Hoarau Vechot J" first="Jessica" last="Hoarau-Véchot">Jessica Hoarau-Véchot</name><affiliation wicri:level="1"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Courjaret, Raphael" sort="Courjaret, Raphael" uniqKey="Courjaret R" first="Raphael" last="Courjaret">Raphael Courjaret</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Machaca, Khaled" sort="Machaca, Khaled" uniqKey="Machaca K" first="Khaled" last="Machaca">Khaled Machaca</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Al Suwaidi, Jassim" sort="Al Suwaidi, Jassim" uniqKey="Al Suwaidi J" first="Jassim" last="Al Suwaidi">Jassim Al Suwaidi</name><affiliation wicri:level="1"><nlm:affiliation>Heart Hospital, Hamad Medical Corporation, Doha, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Heart Hospital, Hamad Medical Corporation, Doha</wicri:regionArea><wicri:noRegion>Doha</wicri:noRegion></affiliation></author><author><name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Rafii, Shahin" sort="Rafii, Shahin" uniqKey="Rafii S" first="Shahin" last="Rafii">Shahin Rafii</name><affiliation wicri:level="2"><nlm:affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Medicine, Weill Cornell Medicine, New York</wicri:cityArea></affiliation></author><author><name sortKey="Elliott, David A" sort="Elliott, David A" uniqKey="Elliott D" first="David A" last="Elliott">David A. Elliott</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Abi Khalil, Charbel" sort="Abi Khalil, Charbel" uniqKey="Abi Khalil C" first="Charbel" last="Abi Khalil">Charbel Abi Khalil</name><affiliation wicri:level="2"><nlm:affiliation>Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar; Heart Hospital, Hamad Medical Corporation, Doha, Qatar; Department of Medicine, Weill Cornell Medicine, New York, New York, USA. Electronic address: cha2022@med.cornell.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar; Heart Hospital, Hamad Medical Corporation, Doha, Qatar; Department of Medicine, Weill Cornell Medicine, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Rafii, Arash" sort="Rafii, Arash" uniqKey="Rafii A" first="Arash" last="Rafii">Arash Rafii</name><affiliation wicri:level="2"><nlm:affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA. Electronic address: jat2021@med.cornell.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><series><title level="j">The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation</title><idno type="eISSN">1557-3117</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pluripotent human embryonic stem cells (hESC) are a promising source of repopulating cardiomyocytes. We hypothesized that we could improve maturation of cardiomyocytes and facilitate electrical interconnections by creating a model that more closely resembles heart tissue; that is, containing both endothelial cells (ECs) and cardiomyocytes.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">28169114</PMID><DateCreated><Year>2017</Year><Month>02</Month><Day>07</Day></DateCreated><DateRevised><Year>2017</Year><Month>05</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1557-3117</ISSN><JournalIssue CitedMedium="Internet"><Volume>36</Volume><Issue>6</Issue><PubDate><Year>2017</Year><Month>Jun</Month></PubDate></JournalIssue><Title>The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation</Title><ISOAbbreviation>J. Heart Lung Transplant.</ISOAbbreviation></Journal><ArticleTitle>Coculturing with endothelial cells promotes in vitro maturation and electrical coupling of human embryonic stem cell-derived cardiomyocytes.</ArticleTitle><Pagination><MedlinePgn>684-693</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1053-2498(17)30008-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.healun.2017.01.001</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Pluripotent human embryonic stem cells (hESC) are a promising source of repopulating cardiomyocytes. We hypothesized that we could improve maturation of cardiomyocytes and facilitate electrical interconnections by creating a model that more closely resembles heart tissue; that is, containing both endothelial cells (ECs) and cardiomyocytes.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We induced cardiomyocyte differentiation in the coculture of an hESC line expressing the cardiac reporter NKX2.5-green fluorescent protein (GFP), and an Akt-activated EC line (E4(+)ECs). We quantified spontaneous beating rates, synchrony, and coordination between different cardiomyocyte clusters using confocal imaging of Fura Red-detected calcium transients and computer-assisted image analysis.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">After 8 days in culture, 94% ± 6% of the NKX2-5GFP(+) cells were beating when hESCs embryonic bodies were plated on E4(+)ECs compared with 34% ± 12.9% for controls consisting of hESCs cultured on BD Matrigel (BD Biosciences) without ECs at Day 11 in culture. The spatial organization of beating areas in cocultures was different. The GFP(+) cardiomyocytes were close to the E4(+)ECs. The average beats/min of the cardiomyocytes in coculture was faster and closer to physiologic heart rates compared with controls (50 ± 14 [n = 13] vs 25 ± 9 [n = 8]; p < 0.05). The coculture with ECs led to synchronized beating relying on the endothelial network, as illustrated by the loss of synchronization upon the disruption of endothelial bridges.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The coculturing of differentiating cardiomyocytes with Akt-activated ECs but not EC-conditioned media results in (1) improved efficiency of the cardiomyocyte differentiation protocol and (2) increased maturity leading to better intercellular coupling with improved chronotropy and synchrony.</AbstractText><CopyrightInformation>Copyright © 2017. Published by Elsevier Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pasquier</LastName><ForeName>Jennifer</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Renuka</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rioult</LastName><ForeName>Damien</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>UMR_I 02 INERIS-URCA-ULH Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims, Reims, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoarau-Véchot</LastName><ForeName>Jessica</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Courjaret</LastName><ForeName>Raphael</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Machaca</LastName><ForeName>Khaled</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Physiology and Biophysics, Weill Cornell Medicine-Qatar, Doha, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Al Suwaidi</LastName><ForeName>Jassim</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Heart Hospital, Hamad Medical Corporation, Doha, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stanley</LastName><ForeName>Edouard G</ForeName><Initials>EG</Initials><AffiliationInfo><Affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rafii</LastName><ForeName>Shahin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elliott</LastName><ForeName>David A</ForeName><Initials>DA</Initials><AffiliationInfo><Affiliation>Department of Medicine, Weill Cornell Medicine, New York, New York; Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abi Khalil</LastName><ForeName>Charbel</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA; Cardiovascular Epigenetics Laboratory, Department of Genetic Medicine, Doha, Qatar; Heart Hospital, Hamad Medical Corporation, Doha, Qatar; Department of Medicine, Weill Cornell Medicine, New York, New York, USA. Electronic address: cha2022@med.cornell.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rafii</LastName><ForeName>Arash</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Stem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Doha, Qatar; Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA. Electronic address: jat2021@med.cornell.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Heart Lung Transplant</MedlineTA><NlmUniqueID>9102703</NlmUniqueID><ISSNLinking>1053-2498</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">cardiomyocytes</Keyword><Keyword MajorTopicYN="N">cardiovascular disease</Keyword><Keyword MajorTopicYN="N">embryonic stem cells</Keyword><Keyword MajorTopicYN="N">endothelial cells</Keyword><Keyword MajorTopicYN="N">vascular niche</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>05</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>12</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28169114</ArticleId><ArticleId IdType="pii">S1053-2498(17)30008-6</ArticleId><ArticleId IdType="doi">10.1016/j.healun.2017.01.001</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>France</li><li>Qatar</li><li>États-Unis</li></country><region><li>Champagne-Ardenne</li><li>Grand Est</li><li>État de New York</li></region><settlement><li>Reims</li></settlement></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></region><name sortKey="Abi Khalil, Charbel" sort="Abi Khalil, Charbel" uniqKey="Abi Khalil C" first="Charbel" last="Abi Khalil">Charbel Abi Khalil</name><name sortKey="Rafii, Arash" sort="Rafii, Arash" uniqKey="Rafii A" first="Arash" last="Rafii">Arash Rafii</name><name sortKey="Rafii, Shahin" sort="Rafii, Shahin" uniqKey="Rafii S" first="Shahin" last="Rafii">Shahin Rafii</name></country><country name="Qatar"><noRegion><name sortKey="Gupta, Renuka" sort="Gupta, Renuka" uniqKey="Gupta R" first="Renuka" last="Gupta">Renuka Gupta</name></noRegion><name sortKey="Al Suwaidi, Jassim" sort="Al Suwaidi, Jassim" uniqKey="Al Suwaidi J" first="Jassim" last="Al Suwaidi">Jassim Al Suwaidi</name><name sortKey="Courjaret, Raphael" sort="Courjaret, Raphael" uniqKey="Courjaret R" first="Raphael" last="Courjaret">Raphael Courjaret</name><name sortKey="Hoarau Vechot, Jessica" sort="Hoarau Vechot, Jessica" uniqKey="Hoarau Vechot J" first="Jessica" last="Hoarau-Véchot">Jessica Hoarau-Véchot</name><name sortKey="Machaca, Khaled" sort="Machaca, Khaled" uniqKey="Machaca K" first="Khaled" last="Machaca">Khaled Machaca</name></country><country name="France"><region name="Grand Est"><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name></region></country><country name="Australie"><noRegion><name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name></noRegion><name sortKey="Elliott, David A" sort="Elliott, David A" uniqKey="Elliott D" first="David A" last="Elliott">David A. Elliott</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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