Intra-Aortic Clusters Undergo Endothelial to Hematopoietic Phenotypic Transition during Early Embryogenesis
Identifieur interne : 000E01 ( Ncbi/Merge ); précédent : 000E00; suivant : 000E02Intra-Aortic Clusters Undergo Endothelial to Hematopoietic Phenotypic Transition during Early Embryogenesis
Auteurs : Chiyo Mizuochi [Japon] ; Stuart T. Fraser [Australie] ; Katia Biasch [France] ; Yuka Horio [Japon] ; Yoshikane Kikushige [Japon] ; Kenzaburo Tani [Japon] ; Koichi Akashi [Japon] ; Manuela Tavian [France] ; Daisuke Sugiyama [Japon]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
Intra-aortic clusters (IACs) attach to floor of large arteries and are considered to have recently acquired hematopoietic stem cell (HSC)-potential in vertebrate early mid-gestation embryos. The formation and function of IACs is poorly understood. To address this issue, IACs were characterized by immunohistochemistry and flow cytometry in mouse embryos. Immunohistochemical analysis revealed that IACs simultaneously express the surface antigens CD31, CD34 and c-Kit. As embryos developed from 9.5 to 10.5 dpc, IACs up-regulate the hematopoietic markers CD41 and CD45 while down-regulating the endothelial surface antigen VE-cadherin/CD144, suggesting that IACs lose endothelial phenotype after 9.5 dpc. Analysis of the hematopoietic potential of IACs revealed a significant change in macrophage CFC activity from 9.5 to 10.5 dpc. To further characterize IACs, we isolated IACs based on CD45 expression. Correspondingly, the expression of hematopoietic transcription factors in the CD45(neg) fraction of IACs was significantly up-regulated. These results suggest that the transition from endothelial to hematopoietic phenotype of IACs occurs after 9.5 dpc.
Url:
DOI: 10.1371/journal.pone.0035763
PubMed: 22558218
PubMed Central: 3338791
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<front><div type="abstract" xml:lang="en"><p>Intra-aortic clusters (IACs) attach to floor of large arteries and are considered to have recently acquired hematopoietic stem cell (HSC)-potential in vertebrate early mid-gestation embryos. The formation and function of IACs is poorly understood. To address this issue, IACs were characterized by immunohistochemistry and flow cytometry in mouse embryos. Immunohistochemical analysis revealed that IACs simultaneously express the surface antigens CD31, CD34 and c-Kit. As embryos developed from 9.5 to 10.5 dpc, IACs up-regulate the hematopoietic markers CD41 and CD45 while down-regulating the endothelial surface antigen VE-cadherin/CD144, suggesting that IACs lose endothelial phenotype after 9.5 dpc. Analysis of the hematopoietic potential of IACs revealed a significant change in macrophage CFC activity from 9.5 to 10.5 dpc. To further characterize IACs, we isolated IACs based on CD45 expression. Correspondingly, the expression of hematopoietic transcription factors in the CD45(neg) fraction of IACs was significantly up-regulated. These results suggest that the transition from endothelial to hematopoietic phenotype of IACs occurs after 9.5 dpc.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22558218</article-id>
<article-id pub-id-type="pmc">3338791</article-id>
<article-id pub-id-type="publisher-id">PONE-D-11-04276</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0035763</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Biology</subject>
<subj-group><subject>Anatomy and Physiology</subject>
</subj-group>
<subj-group><subject>Developmental Biology</subject>
<subj-group><subject>Stem Cells</subject>
<subj-group><subject>Hematopoietic Stem Cells</subject>
</subj-group>
</subj-group>
<subj-group><subject>Cell Differentiation</subject>
<subject>Embryology</subject>
<subject>Molecular Development</subject>
</subj-group>
</subj-group>
<subj-group><subject>Histology</subject>
</subj-group>
<subj-group><subject>Molecular Cell Biology</subject>
<subj-group><subject>Cellular Types</subject>
<subj-group><subject>Stem Cells</subject>
<subj-group><subject>Embryonic Stem Cells</subject>
<subject>Hematopoietic Stem Cells</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject>
<subj-group><subject>Cardiovascular</subject>
<subj-group><subject>Vascular Biology</subject>
</subj-group>
</subj-group>
<subj-group><subject>Hematology</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Intra-Aortic Clusters Undergo Endothelial to Hematopoietic Phenotypic Transition during Early Embryogenesis</article-title>
<alt-title alt-title-type="running-head">Characterization of Intra-Aortic Clusters</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Mizuochi</surname>
<given-names>Chiyo</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fraser</surname>
<given-names>Stuart T.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Biasch</surname>
<given-names>Katia</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Horio</surname>
<given-names>Yuka</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kikushige</surname>
<given-names>Yoshikane</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tani</surname>
<given-names>Kenzaburo</given-names>
</name>
<xref ref-type="aff" rid="aff5"><sup>5</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Akashi</surname>
<given-names>Koichi</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tavian</surname>
<given-names>Manuela</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sugiyama</surname>
<given-names>Daisuke</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Department of Hematopoietic Stem Cells, SSP Stem Cell Unit, Kyushu University Faculty of Medical Sciences, Fukuoka, Japan</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>Laboratory of Blood Cell Development, Disciplines of Physiology, Anatomy and Histology, School of Medical Sciences, University of Sydney, Camperdown, New South Wales, Australia</addr-line>
</aff>
<aff id="aff3"><label>3</label>
<addr-line>Unité 682 INSERM, Strasbourg, France</addr-line>
</aff>
<aff id="aff4"><label>4</label>
<addr-line>Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan</addr-line>
</aff>
<aff id="aff5"><label>5</label>
<addr-line>Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Navarro</surname>
<given-names>Alfons</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1">University of Barcelona, Spain</aff>
<author-notes><corresp id="cor1">* E-mail: <email>ds-mons@yb3.so-net.ne.jp</email>
</corresp>
<fn fn-type="con"><p>Conceived and designed the experiments: DS. Performed the experiments: CM KB YH YK MT DS. Analyzed the data: CM SF KB MT DS. Contributed reagents/materials/analysis tools: CM KB MT KT KA DS. Wrote the paper: CM SF DS.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>27</day>
<month>4</month>
<year>2012</year>
</pub-date>
<volume>7</volume>
<issue>4</issue>
<elocation-id>e35763</elocation-id>
<history><date date-type="received"><day>3</day>
<month>3</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>22</day>
<month>3</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Mizuochi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement>
<copyright-year>2012</copyright-year>
</permissions>
<abstract><p>Intra-aortic clusters (IACs) attach to floor of large arteries and are considered to have recently acquired hematopoietic stem cell (HSC)-potential in vertebrate early mid-gestation embryos. The formation and function of IACs is poorly understood. To address this issue, IACs were characterized by immunohistochemistry and flow cytometry in mouse embryos. Immunohistochemical analysis revealed that IACs simultaneously express the surface antigens CD31, CD34 and c-Kit. As embryos developed from 9.5 to 10.5 dpc, IACs up-regulate the hematopoietic markers CD41 and CD45 while down-regulating the endothelial surface antigen VE-cadherin/CD144, suggesting that IACs lose endothelial phenotype after 9.5 dpc. Analysis of the hematopoietic potential of IACs revealed a significant change in macrophage CFC activity from 9.5 to 10.5 dpc. To further characterize IACs, we isolated IACs based on CD45 expression. Correspondingly, the expression of hematopoietic transcription factors in the CD45(neg) fraction of IACs was significantly up-regulated. These results suggest that the transition from endothelial to hematopoietic phenotype of IACs occurs after 9.5 dpc.</p>
</abstract>
<counts><page-count count="10"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Japon</li>
</country>
<region><li>Alsace (région administrative)</li>
<li>Grand Est</li>
<li>Kyūshū</li>
<li>Nouvelle-Galles du Sud</li>
<li>Préfecture de Fukuoka</li>
</region>
<settlement><li>Fukuoka</li>
<li>Strasbourg</li>
<li>Sydney</li>
</settlement>
<orgName><li>Université de Kyūshū</li>
<li>Université de Sydney</li>
</orgName>
</list>
<tree><country name="Japon"><noRegion><name sortKey="Mizuochi, Chiyo" sort="Mizuochi, Chiyo" uniqKey="Mizuochi C" first="Chiyo" last="Mizuochi">Chiyo Mizuochi</name>
</noRegion>
<name sortKey="Akashi, Koichi" sort="Akashi, Koichi" uniqKey="Akashi K" first="Koichi" last="Akashi">Koichi Akashi</name>
<name sortKey="Horio, Yuka" sort="Horio, Yuka" uniqKey="Horio Y" first="Yuka" last="Horio">Yuka Horio</name>
<name sortKey="Kikushige, Yoshikane" sort="Kikushige, Yoshikane" uniqKey="Kikushige Y" first="Yoshikane" last="Kikushige">Yoshikane Kikushige</name>
<name sortKey="Sugiyama, Daisuke" sort="Sugiyama, Daisuke" uniqKey="Sugiyama D" first="Daisuke" last="Sugiyama">Daisuke Sugiyama</name>
<name sortKey="Tani, Kenzaburo" sort="Tani, Kenzaburo" uniqKey="Tani K" first="Kenzaburo" last="Tani">Kenzaburo Tani</name>
</country>
<country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Fraser, Stuart T" sort="Fraser, Stuart T" uniqKey="Fraser S" first="Stuart T." last="Fraser">Stuart T. Fraser</name>
</region>
</country>
<country name="France"><region name="Grand Est"><name sortKey="Biasch, Katia" sort="Biasch, Katia" uniqKey="Biasch K" first="Katia" last="Biasch">Katia Biasch</name>
</region>
<name sortKey="Tavian, Manuela" sort="Tavian, Manuela" uniqKey="Tavian M" first="Manuela" last="Tavian">Manuela Tavian</name>
</country>
</tree>
</affiliations>
</record>
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