Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes
Identifieur interne : 000433 ( Ncbi/Merge ); précédent : 000432; suivant : 000434Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes
Auteurs : R. A. Gangani D. Silva [États-Unis] ; Rong Huang [États-Unis] ; Jamie Morris [États-Unis] ; Jianwen Fang [États-Unis] ; Elena O. Gracheva [États-Unis] ; Gang Ren [États-Unis] ; Anatol Kontush [France] ; W. Gray Jerome [États-Unis] ; Kerry-Anne Rye [Australie] ; W. Sean Davidson [États-Unis]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2008.
Abstract
Spherical high density lipoproteins (HDL)† predominate in human plasma. However, little information exists on the structure of the most common HDL protein, apolipoprotein (apo) A-I, in spheres vs. better studied discoidal forms. We produced spherical HDL by incubating reconstituted discoidal HDL with physiological plasma-remodeling enzymes and compared apoA-I structure in discs and spheres of comparable diameter (79–80 and 93–96 Å). Using cross-linking chemistry and mass spectrometry, we determined that the general structural organization of apoA-I was overall similar between discs and spheres, regardless of diameter. This was the case despite the fact that the 93 Å spheres contained three molecules of apoA-I per particle compared with only two in the discs. Thus, apoA-I adopts a consistent general structural framework in HDL particles—irrespective of shape, size and the number of apoA-Is present. Furthermore, a similar cross-linking pattern was demonstrated in HDL particles isolated from human serum. We propose the first experiment-based molecular model of apoA-I in spherical HDL particles. This model provides a new foundation for understanding how apoA-I structure modulates HDL function and metabolism.
Url:
DOI: 10.1073/pnas.0803626105
PubMed: 18719128
PubMed Central: 2527885
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Silva</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Morris, Jamie" sort="Morris, Jamie" uniqKey="Morris J" first="Jamie" last="Morris">Jamie Morris</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Fang, Jianwen" sort="Fang, Jianwen" uniqKey="Fang J" first="Jianwen" last="Fang">Jianwen Fang</name><affiliation wicri:level="2"><nlm:aff id="aff2">Applied Bioinformatics Laboratory, University of Kansas, Lawrence, KS 66047;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Kansas</region></placeName><wicri:cityArea>Applied Bioinformatics Laboratory, University of Kansas, Lawrence</wicri:cityArea></affiliation></author><author><name sortKey="Gracheva, Elena O" sort="Gracheva, Elena O" uniqKey="Gracheva E" first="Elena O." last="Gracheva">Elena O. 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Gray Jerome</name><affiliation wicri:level="2"><nlm:aff id="aff5">Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Pathology, Vanderbilt University Medical Center, Nashville</wicri:cityArea></affiliation></author><author><name sortKey="Rye, Kerry Anne" sort="Rye, Kerry Anne" uniqKey="Rye K" first="Kerry-Anne" last="Rye">Kerry-Anne Rye</name><affiliation wicri:level="1"><nlm:aff id="aff6">Lipid Research Group, Heart Research Institute, Camperdown NSW 2050, Australia,</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Lipid Research Group, Heart Research Institute, Camperdown NSW 2050</wicri:regionArea><wicri:noRegion>Camperdown NSW 2050</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff7">**Faculty of Medicine, University of Sydney, NSW 2006, Australia; and</nlm:aff><orgName type="university">Université de Sydney</orgName><country>Australie</country><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff8">Department of Medicine, University of Melbourne, Vic 3010, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Medicine, University of Melbourne, Vic 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Davidson, W Sean" sort="Davidson, W Sean" uniqKey="Davidson W" first="W. 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Sean Davidson</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18719128</idno><idno type="pmc">2527885</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2527885</idno><idno type="RBID">PMC:2527885</idno><idno type="doi">10.1073/pnas.0803626105</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">001B18</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001B18</idno><idno type="wicri:Area/Pmc/Curation">001976</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001976</idno><idno type="wicri:Area/Pmc/Checkpoint">002913</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002913</idno><idno type="wicri:Area/Ncbi/Merge">000433</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes</title><author><name sortKey="Silva, R A Gangani D" sort="Silva, R A Gangani D" uniqKey="Silva R" first="R. A. Gangani D." last="Silva">R. A. Gangani D. Silva</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Morris, Jamie" sort="Morris, Jamie" uniqKey="Morris J" first="Jamie" last="Morris">Jamie Morris</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Fang, Jianwen" sort="Fang, Jianwen" uniqKey="Fang J" first="Jianwen" last="Fang">Jianwen Fang</name><affiliation wicri:level="2"><nlm:aff id="aff2">Applied Bioinformatics Laboratory, University of Kansas, Lawrence, KS 66047;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Kansas</region></placeName><wicri:cityArea>Applied Bioinformatics Laboratory, University of Kansas, Lawrence</wicri:cityArea></affiliation></author><author><name sortKey="Gracheva, Elena O" sort="Gracheva, Elena O" uniqKey="Gracheva E" first="Elena O." last="Gracheva">Elena O. Gracheva</name><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Biochemistry and Biophysics, University of California, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Ren, Gang" sort="Ren, Gang" uniqKey="Ren G" first="Gang" last="Ren">Gang Ren</name><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Biochemistry and Biophysics, University of California, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Kontush, Anatol" sort="Kontush, Anatol" uniqKey="Kontush A" first="Anatol" last="Kontush">Anatol Kontush</name><affiliation wicri:level="3"><nlm:aff id="aff4">Unité Mixte de Recherche S551, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie and Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Pitié-Salpétrière, F-75013 Paris, France;</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unité Mixte de Recherche S551, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie and Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Pitié-Salpétrière, F-75013 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Jerome, W Gray" sort="Jerome, W Gray" uniqKey="Jerome W" first="W. Gray" last="Jerome">W. Gray Jerome</name><affiliation wicri:level="2"><nlm:aff id="aff5">Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Pathology, Vanderbilt University Medical Center, Nashville</wicri:cityArea></affiliation></author><author><name sortKey="Rye, Kerry Anne" sort="Rye, Kerry Anne" uniqKey="Rye K" first="Kerry-Anne" last="Rye">Kerry-Anne Rye</name><affiliation wicri:level="1"><nlm:aff id="aff6">Lipid Research Group, Heart Research Institute, Camperdown NSW 2050, Australia,</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Lipid Research Group, Heart Research Institute, Camperdown NSW 2050</wicri:regionArea><wicri:noRegion>Camperdown NSW 2050</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff7">**Faculty of Medicine, University of Sydney, NSW 2006, Australia; and</nlm:aff><orgName type="university">Université de Sydney</orgName><country>Australie</country><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff8">Department of Medicine, University of Melbourne, Vic 3010, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Medicine, University of Melbourne, Vic 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Davidson, W Sean" sort="Davidson, W Sean" uniqKey="Davidson W" first="W. Sean" last="Davidson">W. Sean Davidson</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati</wicri:cityArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Spherical high density lipoproteins (HDL)<sup>†</sup> predominate in human plasma. However, little information exists on the structure of the most common HDL protein, apolipoprotein (apo) A-I, in spheres vs. better studied discoidal forms. We produced spherical HDL by incubating reconstituted discoidal HDL with physiological plasma-remodeling enzymes and compared apoA-I structure in discs and spheres of comparable diameter (79–80 and 93–96 Å). Using cross-linking chemistry and mass spectrometry, we determined that the general structural organization of apoA-I was overall similar between discs and spheres, regardless of diameter. This was the case despite the fact that the 93 Å spheres contained three molecules of apoA-I per particle compared with only two in the discs. Thus, apoA-I adopts a consistent general structural framework in HDL particles—irrespective of shape, size and the number of apoA-Is present. Furthermore, a similar cross-linking pattern was demonstrated in HDL particles isolated from human serum. We propose the first experiment-based molecular model of apoA-I in spherical HDL particles. This model provides a new foundation for understanding how apoA-I structure modulates HDL function and metabolism.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="hwp">pnas</journal-id><journal-id journal-id-type="pmc">pnas</journal-id><journal-id journal-id-type="publisher-id">PNAS</journal-id><journal-title-group><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title></journal-title-group><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18719128</article-id><article-id pub-id-type="pmc">2527885</article-id><article-id pub-id-type="publisher-id">4414</article-id><article-id pub-id-type="doi">10.1073/pnas.0803626105</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Biochemistry</subject></subj-group></subj-group></article-categories><title-group><article-title>Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Silva</surname><given-names>R. A. Gangani D.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Rong</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Morris</surname><given-names>Jamie</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Fang</surname><given-names>Jianwen</given-names></name><xref ref-type="aff" rid="aff2"><sup>†</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gracheva</surname><given-names>Elena O.</given-names></name><xref ref-type="aff" rid="aff3"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ren</surname><given-names>Gang</given-names></name><xref ref-type="aff" rid="aff3"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kontush</surname><given-names>Anatol</given-names></name><xref ref-type="aff" rid="aff4"><sup>§</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jerome</surname><given-names>W. Gray</given-names></name><xref ref-type="aff" rid="aff5"><sup>¶</sup></xref></contrib><contrib contrib-type="author"><name><surname>Rye</surname><given-names>Kerry-Anne</given-names></name><xref ref-type="aff" rid="aff6"><sup>‖</sup></xref><xref ref-type="aff" rid="aff7">**</xref><xref ref-type="aff" rid="aff8"><sup>††</sup></xref></contrib><contrib contrib-type="author"><name><surname>Davidson</surname><given-names>W. Sean</given-names></name><xref ref-type="aff" rid="aff1">*</xref><xref ref-type="corresp" rid="cor1"><sup>‡‡</sup></xref></contrib><aff id="aff1">*Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237;</aff><aff id="aff2"><sup>†</sup>Applied Bioinformatics Laboratory, University of Kansas, Lawrence, KS 66047;</aff><aff id="aff3"><sup>‡</sup>Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158;</aff><aff id="aff4"><sup>§</sup>Unité Mixte de Recherche S551, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie and Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Pitié-Salpétrière, F-75013 Paris, France;</aff><aff id="aff5"><sup>¶</sup>Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232;</aff><aff id="aff6"><sup>‖</sup>Lipid Research Group, Heart Research Institute, Camperdown NSW 2050, Australia,</aff><aff id="aff7">**Faculty of Medicine, University of Sydney, NSW 2006, Australia; and</aff><aff id="aff8"><sup>††</sup>Department of Medicine, University of Melbourne, Vic 3010, Australia</aff></contrib-group><author-notes><corresp id="cor1"><sup>‡‡</sup>To whom correspondence may be addressed. E-mail: <email>sean.davidson@uc.edu</email></corresp><fn fn-type="edited-by"><p>Edited by Richard J. Havel, University of California School of Medicine, San Francisco, CA, and approved June 21, 2008</p></fn><fn fn-type="con"><p>Author contributions: R.A.G.D.S. and W.S.D. designed research; R.A.G.D.S., R.H., J.M., E.O.G., G.R., A.K., W.G.J., K.-A.R., and W.S.D. performed research; R.A.G.D.S., R.H., J.F., W.G.J., and W.S.D. analyzed data; and R.A.G.D.S. and W.S.D. wrote the paper.</p></fn></author-notes><pub-date pub-type="ppub"><day>26</day><month>8</month><year>2008</year></pub-date><pub-date pub-type="epub"><day>21</day><month>8</month><year>2008</year></pub-date><volume>105</volume><issue>34</issue><fpage>12176</fpage><lpage>12181</lpage><history><date date-type="received"><day>14</day><month>4</month><year>2008</year></date></history><permissions><copyright-statement>© 2008 by The National Academy of Sciences of the USA</copyright-statement></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zpq03408012176.pdf"></self-uri><abstract><p>Spherical high density lipoproteins (HDL)<sup>†</sup> predominate in human plasma. However, little information exists on the structure of the most common HDL protein, apolipoprotein (apo) A-I, in spheres vs. better studied discoidal forms. We produced spherical HDL by incubating reconstituted discoidal HDL with physiological plasma-remodeling enzymes and compared apoA-I structure in discs and spheres of comparable diameter (79–80 and 93–96 Å). Using cross-linking chemistry and mass spectrometry, we determined that the general structural organization of apoA-I was overall similar between discs and spheres, regardless of diameter. This was the case despite the fact that the 93 Å spheres contained three molecules of apoA-I per particle compared with only two in the discs. Thus, apoA-I adopts a consistent general structural framework in HDL particles—irrespective of shape, size and the number of apoA-Is present. Furthermore, a similar cross-linking pattern was demonstrated in HDL particles isolated from human serum. We propose the first experiment-based molecular model of apoA-I in spherical HDL particles. This model provides a new foundation for understanding how apoA-I structure modulates HDL function and metabolism.</p></abstract><kwd-group><kwd>sphere</kwd><kwd>disk</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Australie</li><li>France</li><li>États-Unis</li></country><region><li>Californie</li><li>Kansas</li><li>Nouvelle-Galles du Sud</li><li>Ohio</li><li>Tennessee</li><li>Victoria (État)</li><li>Île-de-France</li></region><settlement><li>Melbourne</li><li>Paris</li><li>Sydney</li></settlement><orgName><li>Université de Melbourne</li><li>Université de Sydney</li></orgName></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Silva, R A Gangani D" sort="Silva, R A Gangani D" uniqKey="Silva R" first="R. A. Gangani D." last="Silva">R. A. Gangani D. Silva</name></region><name sortKey="Davidson, W Sean" sort="Davidson, W Sean" uniqKey="Davidson W" first="W. Sean" last="Davidson">W. Sean Davidson</name><name sortKey="Fang, Jianwen" sort="Fang, Jianwen" uniqKey="Fang J" first="Jianwen" last="Fang">Jianwen Fang</name><name sortKey="Gracheva, Elena O" sort="Gracheva, Elena O" uniqKey="Gracheva E" first="Elena O." last="Gracheva">Elena O. Gracheva</name><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><name sortKey="Jerome, W Gray" sort="Jerome, W Gray" uniqKey="Jerome W" first="W. Gray" last="Jerome">W. Gray Jerome</name><name sortKey="Morris, Jamie" sort="Morris, Jamie" uniqKey="Morris J" first="Jamie" last="Morris">Jamie Morris</name><name sortKey="Ren, Gang" sort="Ren, Gang" uniqKey="Ren G" first="Gang" last="Ren">Gang Ren</name></country><country name="France"><region name="Île-de-France"><name sortKey="Kontush, Anatol" sort="Kontush, Anatol" uniqKey="Kontush A" first="Anatol" last="Kontush">Anatol Kontush</name></region></country><country name="Australie"><noRegion><name sortKey="Rye, Kerry Anne" sort="Rye, Kerry Anne" uniqKey="Rye K" first="Kerry-Anne" last="Rye">Kerry-Anne Rye</name></noRegion><name sortKey="Rye, Kerry Anne" sort="Rye, Kerry Anne" uniqKey="Rye K" first="Kerry-Anne" last="Rye">Kerry-Anne Rye</name><name sortKey="Rye, Kerry Anne" sort="Rye, Kerry Anne" uniqKey="Rye K" first="Kerry-Anne" last="Rye">Kerry-Anne Rye</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|clé= PMC:2527885
|texte= Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes
}}
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| NlmPubMed2Wicri -a AustralieFrV1
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