ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria
Identifieur interne : 001C93 ( Ncbi/Curation ); précédent : 001C92; suivant : 001C94ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria
Auteurs : Andrew N. Keller [Australie] ; Xiao Yang [Australie] ; Jana Wiedermannová [République tchèque] ; Olivier Delumeau [France] ; Libor Krásn [République tchèque] ; Peter J. Lewis [Australie]Source :
- Journal of Bacteriology [ 0021-9193 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Bacillus subtilis (enzymologie), Conformation des protéines, DNA-directed RNA polymerases (), DNA-directed RNA polymerases (génétique), DNA-directed RNA polymerases (métabolisme), Données de séquences moléculaires, Modèles moléculaires, Phylogénie, Régulation de l'expression des gènes bactériens, Régulation de l'expression des gènes codant pour des enzymes, Sous-unités de protéines, Séquence d'acides aminés.
- MESH :
- enzymologie : Bacillus subtilis.
- génétique : DNA-directed RNA polymerases.
- métabolisme : DNA-directed RNA polymerases.
- Animaux, Conformation des protéines, DNA-directed RNA polymerases, Données de séquences moléculaires, Modèles moléculaires, Phylogénie, Régulation de l'expression des gènes bactériens, Régulation de l'expression des gènes codant pour des enzymes, Sous-unités de protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Bacillus subtilis (enzymology), DNA-Directed RNA Polymerases (chemistry), DNA-Directed RNA Polymerases (genetics), DNA-Directed RNA Polymerases (metabolism), Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Models, Molecular, Molecular Sequence Data, Phylogeny, Protein Conformation, Protein Subunits.
- MESH :
- chemical , chemistry : DNA-Directed RNA Polymerases.
- enzymology : Bacillus subtilis.
- chemical , genetics : DNA-Directed RNA Polymerases.
- chemical , metabolism : DNA-Directed RNA Polymerases.
- Amino Acid Sequence, Animals, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Models, Molecular, Molecular Sequence Data, Phylogeny, Protein Conformation, Protein Subunits.
Abstract
RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T
Url:
DOI: 10.1128/JB.02020-14
PubMed: 25092033
PubMed Central: 4187704
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001911
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001770
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001257
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :003394
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :003282
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003282
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001C93
Links to Exploration step
PMC:4187704Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria</title>
<author><name sortKey="Keller, Andrew N" sort="Keller, Andrew N" uniqKey="Keller A" first="Andrew N." last="Keller">Andrew N. Keller</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales</wicri:regionArea>
<wicri:noRegion>New South Wales</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yang, Xiao" sort="Yang, Xiao" uniqKey="Yang X" first="Xiao" last="Yang">Xiao Yang</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales</wicri:regionArea>
<wicri:noRegion>New South Wales</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wiedermannova, Jana" sort="Wiedermannova, Jana" uniqKey="Wiedermannova J" first="Jana" last="Wiedermannová">Jana Wiedermannová</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic</nlm:aff>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague</wicri:regionArea>
<placeName><settlement type="city">Prague</settlement>
<region type="région" nuts="2">Bohême centrale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Delumeau, Olivier" sort="Delumeau, Olivier" uniqKey="Delumeau O" first="Olivier" last="Delumeau">Olivier Delumeau</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">INRA, UMR-1319 Micalis, Jouy-en-Josas, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>INRA, UMR-1319 Micalis, Jouy-en-Josas</wicri:regionArea>
<wicri:noRegion>Jouy-en-Josas</wicri:noRegion>
<wicri:noRegion>Jouy-en-Josas</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Krasn, Libor" sort="Krasn, Libor" uniqKey="Krasn L" first="Libor" last="Krásn">Libor Krásn</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic</nlm:aff>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague</wicri:regionArea>
<placeName><settlement type="city">Prague</settlement>
<region type="région" nuts="2">Bohême centrale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lewis, Peter J" sort="Lewis, Peter J" uniqKey="Lewis P" first="Peter J." last="Lewis">Peter J. Lewis</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales</wicri:regionArea>
<wicri:noRegion>New South Wales</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25092033</idno>
<idno type="pmc">4187704</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187704</idno>
<idno type="RBID">PMC:4187704</idno>
<idno type="doi">10.1128/JB.02020-14</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">001911</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001911</idno>
<idno type="wicri:Area/Pmc/Curation">001770</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001770</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001257</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001257</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">003394</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003394</idno>
<idno type="wicri:Area/PubMed/Curation">003282</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003282</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003282</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003282</idno>
<idno type="wicri:Area/Ncbi/Merge">001C93</idno>
<idno type="wicri:Area/Ncbi/Curation">001C93</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria</title>
<author><name sortKey="Keller, Andrew N" sort="Keller, Andrew N" uniqKey="Keller A" first="Andrew N." last="Keller">Andrew N. Keller</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales</wicri:regionArea>
<wicri:noRegion>New South Wales</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yang, Xiao" sort="Yang, Xiao" uniqKey="Yang X" first="Xiao" last="Yang">Xiao Yang</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales</wicri:regionArea>
<wicri:noRegion>New South Wales</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wiedermannova, Jana" sort="Wiedermannova, Jana" uniqKey="Wiedermannova J" first="Jana" last="Wiedermannová">Jana Wiedermannová</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic</nlm:aff>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague</wicri:regionArea>
<placeName><settlement type="city">Prague</settlement>
<region type="région" nuts="2">Bohême centrale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Delumeau, Olivier" sort="Delumeau, Olivier" uniqKey="Delumeau O" first="Olivier" last="Delumeau">Olivier Delumeau</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">INRA, UMR-1319 Micalis, Jouy-en-Josas, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>INRA, UMR-1319 Micalis, Jouy-en-Josas</wicri:regionArea>
<wicri:noRegion>Jouy-en-Josas</wicri:noRegion>
<wicri:noRegion>Jouy-en-Josas</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Krasn, Libor" sort="Krasn, Libor" uniqKey="Krasn L" first="Libor" last="Krásn">Libor Krásn</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic</nlm:aff>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague</wicri:regionArea>
<placeName><settlement type="city">Prague</settlement>
<region type="région" nuts="2">Bohême centrale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lewis, Peter J" sort="Lewis, Peter J" uniqKey="Lewis P" first="Peter J." last="Lewis">Peter J. Lewis</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales</wicri:regionArea>
<wicri:noRegion>New South Wales</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Bacteriology</title>
<idno type="ISSN">0021-9193</idno>
<idno type="eISSN">1098-5530</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Bacillus subtilis (enzymology)</term>
<term>DNA-Directed RNA Polymerases (chemistry)</term>
<term>DNA-Directed RNA Polymerases (genetics)</term>
<term>DNA-Directed RNA Polymerases (metabolism)</term>
<term>Gene Expression Regulation, Bacterial</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Protein Conformation</term>
<term>Protein Subunits</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Bacillus subtilis (enzymologie)</term>
<term>Conformation des protéines</term>
<term>DNA-directed RNA polymerases ()</term>
<term>DNA-directed RNA polymerases (génétique)</term>
<term>DNA-directed RNA polymerases (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Modèles moléculaires</term>
<term>Phylogénie</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Régulation de l'expression des gènes codant pour des enzymes</term>
<term>Sous-unités de protéines</term>
<term>Séquence d'acides aminés</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA-Directed RNA Polymerases</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bacillus subtilis</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bacillus subtilis</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA-Directed RNA Polymerases</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>DNA-directed RNA polymerases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Directed RNA Polymerases</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>DNA-directed RNA polymerases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Gene Expression Regulation, Bacterial</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Protein Conformation</term>
<term>Protein Subunits</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Conformation des protéines</term>
<term>DNA-directed RNA polymerases</term>
<term>Données de séquences moléculaires</term>
<term>Modèles moléculaires</term>
<term>Phylogénie</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Régulation de l'expression des gènes codant pour des enzymes</term>
<term>Sous-unités de protéines</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T <named-content content-type="genus-species">Firmicutes</named-content>
phylum of Gram-positive bacteria and have named it ε. Previously ε had been identified as a small protein (ω<sub>1</sub>
) that copurified with RNA polymerase. We have solved the structure of ε by X-ray crystallography and show that it is not an ω subunit. Rather, ε bears remarkable similarity to the Gp2 family of phage proteins involved in the inhibition of host cell transcription following infection. Deletion of ε shows no phenotype and has no effect on the transcriptional profile of the cell. Determination of the location of ε within the assembly of RNA polymerase core by single-particle analysis suggests that it binds toward the downstream side of the DNA binding cleft. Due to the structural similarity of ε with Gp2 and the fact they bind similar regions of RNA polymerase, we hypothesize that ε may serve a role in protection from phage infection.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001C93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:4187704 |texte= ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:25092033" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |