AustralieFrV1, Ncbi, Checkpoint, bibRecord, 003F13

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

Identifieur interne : 003F13 ( Ncbi/Checkpoint ); précédent : 003F12; suivant : 003F14

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

Auteurs : Michael J. Workman [États-Unis] ; Maxime M. Mahe [États-Unis] ; Stephen Trisno [États-Unis] ; Holly M. Poling [États-Unis] ; Carey L. Watson [États-Unis] ; Nambirajan Sundaram [États-Unis] ; Ching-Fang Chang [États-Unis] ; Jacqueline Schiesser [États-Unis] ; Philippe Aubert [France] ; Edouard G. Stanley [Australie] ; Andrew G. Elefanty [Australie] ; Yuichiro Miyaoka [États-Unis] ; Mohammad A. Mandegar [États-Unis] ; Bruce R. Conklin [États-Unis] ; Michel Neunlist [France] ; Samantha A. Brugmann [États-Unis] ; Michael A. Helmrath [États-Unis] ; James M. Wells [États-Unis]

Source :

Nature medicine [ 1078-8956 ] ; 2016.

RBID : PMC:5562951

Descripteurs français

English descriptors

KwdEn :
- Animals, Calcium (metabolism), Cell Line, Chick Embryo, Enteric Nervous System (physiology), Enteric Nervous System (physiopathology), Gastrointestinal Motility, Hirschsprung Disease (genetics), Hirschsprung Disease (physiopathology), Homeodomain Proteins (genetics), Humans, Immunohistochemistry, In Vitro Techniques, Induced Pluripotent Stem Cells, Interstitial Cells of Cajal (physiology), Intestines (physiology), Intestines (physiopathology), Mice, Mice, SCID, Microscopy, Confocal, Models, Biological, Mutation, Myenteric Plexus (physiology), Myenteric Plexus (physiopathology), Neural Crest, Neurogenesis (physiology), Neuroglia (physiology), Neurons (physiology), Organoids, Permeability, Real-Time Polymerase Chain Reaction, Submucous Plexus (physiology), Submucous Plexus (physiopathology), Tissue Engineering (methods), Transcription Factors (genetics).
MESH :
- chemical , genetics : Homeodomain Proteins, Transcription Factors.
- chemical , metabolism : Calcium.
- genetics : Hirschsprung Disease.
- methods : Tissue Engineering.
- physiology : Enteric Nervous System, Interstitial Cells of Cajal, Intestines, Myenteric Plexus, Neurogenesis, Neuroglia, Neurons, Submucous Plexus.
- physiopathology : Enteric Nervous System, Hirschsprung Disease, Intestines, Myenteric Plexus, Submucous Plexus.
- Animals, Cell Line, Chick Embryo, Gastrointestinal Motility, Humans, Immunohistochemistry, In Vitro Techniques, Induced Pluripotent Stem Cells, Mice, Mice, SCID, Microscopy, Confocal, Models, Biological, Mutation, Neural Crest, Organoids, Permeability, Real-Time Polymerase Chain Reaction.

Abstract

The enteric nervous system (ENS) of the gastrointestinal tract controls many diverse functions, including motility and epithelial permeability. Perturbations in ENS development or function are common, yet there is no human model for studying ENS-intestinal biology and disease. We used a tissue-engineering approach with embryonic and induced pluripotent stem cells (PSCs) to generate human intestinal tissue containing a functional ENS. We recapitulated normal intestinal ENS development by combining human-PSC-derived neural crest cells (NCCs) and developing human intestinal organoids (HIOs). NCCs recombined with HIOs in vitro migrated into the mesenchyme, differentiated into neurons and glial cells and showed neuronal activity, as measured by rhythmic waves of calcium transients. ENS-containing HIOs grown in vivo formed neuroglial structures similar to a myenteric and submucosal plexus, had functional interstitial cells of Cajal and had an electromechanical coupling that regulated waves of propagating contraction. Finally, we used this system to investigate the cellular and molecular basis for Hirschsprung's disease caused by a mutation in the gene PHOX2B. This is, to the best of our knowledge, the first demonstration of human-PSC-derived intestinal tissue with a functional ENS and how this system can be used to study motility disorders of the human gastrointestinal tract.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562951

DOI: 10.1038/nm.4233
PubMed: 27869805
PubMed Central: 5562951

Affiliations:

Links to Exploration step

PMC:5562951

Le document en format XML

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<author><name sortKey="Schiesser, Jacqueline" sort="Schiesser, Jacqueline" uniqKey="Schiesser J" first="Jacqueline" last="Schiesser">Jacqueline Schiesser</name>
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<author><name sortKey="Aubert, Philippe" sort="Aubert, Philippe" uniqKey="Aubert P" first="Philippe" last="Aubert">Philippe Aubert</name>
<affiliation wicri:level="3"><nlm:aff id="A4">INSERM UMR 913, University of Nantes, CHU de Nantes—Institut des Maladies de l'Appareil Digestif, Nantes, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM UMR 913, University of Nantes, CHU de Nantes—Institut des Maladies de l'Appareil Digestif, Nantes</wicri:regionArea>
<placeName><region type="region">Pays de la Loire</region>
<region type="old region">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name>
<affiliation wicri:level="1"><nlm:aff id="A5">Murdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Murdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="A6">Department of Pediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Pediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A7">Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Elefanty, Andrew G" sort="Elefanty, Andrew G" uniqKey="Elefanty A" first="Andrew G" last="Elefanty">Andrew G. Elefanty</name>
<affiliation wicri:level="1"><nlm:aff id="A5">Murdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Murdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="A6">Department of Pediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Pediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A7">Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Miyaoka, Yuichiro" sort="Miyaoka, Yuichiro" uniqKey="Miyaoka Y" first="Yuichiro" last="Miyaoka">Yuichiro Miyaoka</name>
<affiliation wicri:level="2"><nlm:aff id="A8">Gladstone Institutes, San Francisco, California, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Gladstone Institutes, San Francisco, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mandegar, Mohammad A" sort="Mandegar, Mohammad A" uniqKey="Mandegar M" first="Mohammad A" last="Mandegar">Mohammad A. Mandegar</name>
<affiliation wicri:level="2"><nlm:aff id="A8">Gladstone Institutes, San Francisco, California, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Gladstone Institutes, San Francisco, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Conklin, Bruce R" sort="Conklin, Bruce R" uniqKey="Conklin B" first="Bruce R" last="Conklin">Bruce R. Conklin</name>
<affiliation wicri:level="2"><nlm:aff id="A8">Gladstone Institutes, San Francisco, California, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Gladstone Institutes, San Francisco, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="A9">Departments of Medicine and Molecular and Cellular Pharmacology, University of California at San Francisco, San Francisco, California, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Departments of Medicine and Molecular and Cellular Pharmacology, University of California at San Francisco, San Francisco, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name>
<affiliation wicri:level="3"><nlm:aff id="A4">INSERM UMR 913, University of Nantes, CHU de Nantes—Institut des Maladies de l'Appareil Digestif, Nantes, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM UMR 913, University of Nantes, CHU de Nantes—Institut des Maladies de l'Appareil Digestif, Nantes</wicri:regionArea>
<placeName><region type="region">Pays de la Loire</region>
<region type="old region">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Brugmann, Samantha A" sort="Brugmann, Samantha A" uniqKey="Brugmann S" first="Samantha A" last="Brugmann">Samantha A. Brugmann</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="A3">Division of Plastic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Plastic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Helmrath, Michael A" sort="Helmrath, Michael A" uniqKey="Helmrath M" first="Michael A" last="Helmrath">Michael A. Helmrath</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wells, James M" sort="Wells, James M" uniqKey="Wells J" first="James M" last="Wells">James M. Wells</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="A10">Division of Endocrinology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Endocrinology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Nature medicine</title>
<idno type="ISSN">1078-8956</idno>
<idno type="eISSN">1546-170X</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Calcium (metabolism)</term>
<term>Cell Line</term>
<term>Chick Embryo</term>
<term>Enteric Nervous System (physiology)</term>
<term>Enteric Nervous System (physiopathology)</term>
<term>Gastrointestinal Motility</term>
<term>Hirschsprung Disease (genetics)</term>
<term>Hirschsprung Disease (physiopathology)</term>
<term>Homeodomain Proteins (genetics)</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>In Vitro Techniques</term>
<term>Induced Pluripotent Stem Cells</term>
<term>Interstitial Cells of Cajal (physiology)</term>
<term>Intestines (physiology)</term>
<term>Intestines (physiopathology)</term>
<term>Mice</term>
<term>Mice, SCID</term>
<term>Microscopy, Confocal</term>
<term>Models, Biological</term>
<term>Mutation</term>
<term>Myenteric Plexus (physiology)</term>
<term>Myenteric Plexus (physiopathology)</term>
<term>Neural Crest</term>
<term>Neurogenesis (physiology)</term>
<term>Neuroglia (physiology)</term>
<term>Neurons (physiology)</term>
<term>Organoids</term>
<term>Permeability</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Submucous Plexus (physiology)</term>
<term>Submucous Plexus (physiopathology)</term>
<term>Tissue Engineering (methods)</term>
<term>Transcription Factors (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Calcium (métabolisme)</term>
<term>Cellules interstitielles de Cajal (physiologie)</term>
<term>Cellules souches pluripotentes induites</term>
<term>Crête neurale</term>
<term>Embryon de poulet</term>
<term>Facteurs de transcription (génétique)</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Ingénierie tissulaire ()</term>
<term>Intestins (physiologie)</term>
<term>Intestins (physiopathologie)</term>
<term>Lignée cellulaire</term>
<term>Maladie de Hirschsprung (génétique)</term>
<term>Maladie de Hirschsprung (physiopathologie)</term>
<term>Microscopie confocale</term>
<term>Modèles biologiques</term>
<term>Motilité gastrointestinale</term>
<term>Mutation</term>
<term>Neurogenèse (physiologie)</term>
<term>Neurones (physiologie)</term>
<term>Névroglie (physiologie)</term>
<term>Organoïdes</term>
<term>Perméabilité</term>
<term>Plexus myentérique (physiologie)</term>
<term>Plexus myentérique (physiopathologie)</term>
<term>Plexus nerveux sous-muqueux (physiologie)</term>
<term>Plexus nerveux sous-muqueux (physiopathologie)</term>
<term>Protéines à homéodomaine (génétique)</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Souris</term>
<term>Souris SCID</term>
<term>Système nerveux entérique (physiologie)</term>
<term>Système nerveux entérique (physiopathologie)</term>
<term>Techniques in vitro</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Homeodomain Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hirschsprung Disease</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Maladie de Hirschsprung</term>
<term>Protéines à homéodomaine</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Tissue Engineering</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calcium</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules interstitielles de Cajal</term>
<term>Intestins</term>
<term>Neurogenèse</term>
<term>Neurones</term>
<term>Névroglie</term>
<term>Plexus myentérique</term>
<term>Plexus nerveux sous-muqueux</term>
<term>Système nerveux entérique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Enteric Nervous System</term>
<term>Interstitial Cells of Cajal</term>
<term>Intestines</term>
<term>Myenteric Plexus</term>
<term>Neurogenesis</term>
<term>Neuroglia</term>
<term>Neurons</term>
<term>Submucous Plexus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Intestins</term>
<term>Maladie de Hirschsprung</term>
<term>Plexus myentérique</term>
<term>Plexus nerveux sous-muqueux</term>
<term>Système nerveux entérique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Enteric Nervous System</term>
<term>Hirschsprung Disease</term>
<term>Intestines</term>
<term>Myenteric Plexus</term>
<term>Submucous Plexus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chick Embryo</term>
<term>Gastrointestinal Motility</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>In Vitro Techniques</term>
<term>Induced Pluripotent Stem Cells</term>
<term>Mice</term>
<term>Mice, SCID</term>
<term>Microscopy, Confocal</term>
<term>Models, Biological</term>
<term>Mutation</term>
<term>Neural Crest</term>
<term>Organoids</term>
<term>Permeability</term>
<term>Real-Time Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules souches pluripotentes induites</term>
<term>Crête neurale</term>
<term>Embryon de poulet</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Ingénierie tissulaire</term>
<term>Lignée cellulaire</term>
<term>Microscopie confocale</term>
<term>Modèles biologiques</term>
<term>Motilité gastrointestinale</term>
<term>Mutation</term>
<term>Organoïdes</term>
<term>Perméabilité</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Souris</term>
<term>Souris SCID</term>
<term>Techniques in vitro</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">The enteric nervous system (ENS) of the gastrointestinal tract controls many diverse functions, including motility and epithelial permeability. Perturbations in ENS development or function are common, yet there is no human model for studying ENS-intestinal biology and disease. We used a tissue-engineering approach with embryonic and induced pluripotent stem cells (PSCs) to generate human intestinal tissue containing a functional ENS. We recapitulated normal intestinal ENS development by combining human-PSC-derived neural crest cells (NCCs) and developing human intestinal organoids (HIOs). NCCs recombined with HIOs <italic>in vitro</italic>
 migrated into the mesenchyme, differentiated into neurons and glial cells and showed neuronal activity, as measured by rhythmic waves of calcium transients. ENS-containing HIOs grown <italic>in vivo</italic>
 formed neuroglial structures similar to a myenteric and submucosal plexus, had functional interstitial cells of Cajal and had an electromechanical coupling that regulated waves of propagating contraction. Finally, we used this system to investigate the cellular and molecular basis for Hirschsprung's disease caused by a mutation in the gene <italic>PHOX2B</italic>
. This is, to the best of our knowledge, the first demonstration of human-PSC-derived intestinal tissue with a functional ENS and how this system can be used to study motility disorders of the human gastrointestinal tract.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Ohio</li>
<li>Pays de la Loire</li>
<li>Victoria (État)</li>
</region>
<settlement><li>Melbourne</li>
<li>Nantes</li>
</settlement>
<orgName><li>Université de Melbourne</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Ohio"><name sortKey="Workman, Michael J" sort="Workman, Michael J" uniqKey="Workman M" first="Michael J" last="Workman">Michael J. Workman</name>
</region>
<name sortKey="Brugmann, Samantha A" sort="Brugmann, Samantha A" uniqKey="Brugmann S" first="Samantha A" last="Brugmann">Samantha A. Brugmann</name>
<name sortKey="Brugmann, Samantha A" sort="Brugmann, Samantha A" uniqKey="Brugmann S" first="Samantha A" last="Brugmann">Samantha A. Brugmann</name>
<name sortKey="Chang, Ching Fang" sort="Chang, Ching Fang" uniqKey="Chang C" first="Ching-Fang" last="Chang">Ching-Fang Chang</name>
<name sortKey="Chang, Ching Fang" sort="Chang, Ching Fang" uniqKey="Chang C" first="Ching-Fang" last="Chang">Ching-Fang Chang</name>
<name sortKey="Conklin, Bruce R" sort="Conklin, Bruce R" uniqKey="Conklin B" first="Bruce R" last="Conklin">Bruce R. Conklin</name>
<name sortKey="Conklin, Bruce R" sort="Conklin, Bruce R" uniqKey="Conklin B" first="Bruce R" last="Conklin">Bruce R. Conklin</name>
<name sortKey="Helmrath, Michael A" sort="Helmrath, Michael A" uniqKey="Helmrath M" first="Michael A" last="Helmrath">Michael A. Helmrath</name>
<name sortKey="Mahe, Maxime M" sort="Mahe, Maxime M" uniqKey="Mahe M" first="Maxime M" last="Mahe">Maxime M. Mahe</name>
<name sortKey="Mandegar, Mohammad A" sort="Mandegar, Mohammad A" uniqKey="Mandegar M" first="Mohammad A" last="Mandegar">Mohammad A. Mandegar</name>
<name sortKey="Miyaoka, Yuichiro" sort="Miyaoka, Yuichiro" uniqKey="Miyaoka Y" first="Yuichiro" last="Miyaoka">Yuichiro Miyaoka</name>
<name sortKey="Poling, Holly M" sort="Poling, Holly M" uniqKey="Poling H" first="Holly M" last="Poling">Holly M. Poling</name>
<name sortKey="Schiesser, Jacqueline" sort="Schiesser, Jacqueline" uniqKey="Schiesser J" first="Jacqueline" last="Schiesser">Jacqueline Schiesser</name>
<name sortKey="Sundaram, Nambirajan" sort="Sundaram, Nambirajan" uniqKey="Sundaram N" first="Nambirajan" last="Sundaram">Nambirajan Sundaram</name>
<name sortKey="Trisno, Stephen" sort="Trisno, Stephen" uniqKey="Trisno S" first="Stephen" last="Trisno">Stephen Trisno</name>
<name sortKey="Watson, Carey L" sort="Watson, Carey L" uniqKey="Watson C" first="Carey L" last="Watson">Carey L. Watson</name>
<name sortKey="Wells, James M" sort="Wells, James M" uniqKey="Wells J" first="James M" last="Wells">James M. Wells</name>
<name sortKey="Wells, James M" sort="Wells, James M" uniqKey="Wells J" first="James M" last="Wells">James M. Wells</name>
</country>
<country name="France"><region name="Pays de la Loire"><name sortKey="Aubert, Philippe" sort="Aubert, Philippe" uniqKey="Aubert P" first="Philippe" last="Aubert">Philippe Aubert</name>
</region>
<name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name>
</country>
<country name="Australie"><noRegion><name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name>
</noRegion>
<name sortKey="Elefanty, Andrew G" sort="Elefanty, Andrew G" uniqKey="Elefanty A" first="Andrew G" last="Elefanty">Andrew G. Elefanty</name>
<name sortKey="Elefanty, Andrew G" sort="Elefanty, Andrew G" uniqKey="Elefanty A" first="Andrew G" last="Elefanty">Andrew G. Elefanty</name>
<name sortKey="Elefanty, Andrew G" sort="Elefanty, Andrew G" uniqKey="Elefanty A" first="Andrew G" last="Elefanty">Andrew G. Elefanty</name>
<name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name>
<name sortKey="Stanley, Edouard G" sort="Stanley, Edouard G" uniqKey="Stanley E" first="Edouard G" last="Stanley">Edouard G. Stanley</name>
</country>
</tree>
</affiliations>
</record>

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Serveur d'exploration sur les relations entre la France et l'Australie

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

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