New insights for identification of doping with recombinant human erythropoietin micro-doses after high hydration.
Identifieur interne : 003814 ( Ncbi/Checkpoint ); précédent : 003813; suivant : 003815New insights for identification of doping with recombinant human erythropoietin micro-doses after high hydration.
Auteurs : L. Martin [France] ; M. Ashenden [Australie] ; J. Bejder [Danemark] ; M. Hoffmann [Danemark] ; N. Nordsborg [Danemark] ; K. Karstoft [Danemark] ; J. Morkeberg [Danemark] ; K. Sharpe [Australie] ; F. Lasne [France] ; A. Marchand [France]Source :
- Drug testing and analysis [ 1942-7611 ] ; 2016.
Descripteurs français
- KwdFr :
- Détection d'abus de substances (), Focalisation isoélectrique (), Humains, Protéines recombinantes (métabolisme), Protéines recombinantes (sang), Protéines recombinantes (urine), Électrophorèse sur gel de polyacrylamide (), Érythropoïétine (métabolisme), Érythropoïétine (sang), Érythropoïétine (urine).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Erythropoietin, Recombinant Proteins.
- chemical , metabolism : Erythropoietin, Recombinant Proteins.
- methods : Electrophoresis, Polyacrylamide Gel, Isoelectric Focusing, Substance Abuse Detection.
- chemical , urine : Erythropoietin, Recombinant Proteins.
- Humans.
Abstract
To minimize the chances of being caught after doping with recombinant human erythropoietins (rhEPO), athletes have turned to new practices using micro-doses and excess fluid ingestion to accelerate elimination and decrease the probability of detection. Our objective was to test the sensitivity of detection by validated methods (IEF: isoelectric focusing; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis) when such practices are used. First, after a three-week rhEPO boost period and 10 days of wash out, detection of a single 900 IU micro-dose of Eprex® was evaluated in healthy male subjects. After an injection in the evening, urine and plasma samples were collected the following morning. Half of the subjects then drank a bolus of water and new samples were collected 80 min later. Interestingly, rhEPO was detected in 100% of the samples even after water ingestion. A second similar protocol was then performed with a single injection of a micro-dose of rhEPO (500 IU or 900 IU), without a prior rhEPO boost. In addition, urine and plasma samples were also collected 15 and 20 h post rhEPO administration. Once again drinking water did not affect the rate of detection. Urine appeared a better matrix to detect micro-doses after 10 h, enabling between 92% and 100% of identification at that time. The rate of identification decreased rapidly thereafter, in particular for the 500 IU micro-dose. However IEF analysis still resulted in 71% identification of rhEPO in urine after 20 h. These results could help to define a better strategy for controlling and identifying athletes using rhEPO micro-doses. Copyright © 2016 John Wiley & Sons, Ltd.
DOI: 10.1002/dta.2004
PubMed: 27390252
Affiliations:
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<term>Erythropoietin (metabolism)</term>
<term>Erythropoietin (urine)</term>
<term>Humans</term>
<term>Isoelectric Focusing (methods)</term>
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<front><div type="abstract" xml:lang="en">To minimize the chances of being caught after doping with recombinant human erythropoietins (rhEPO), athletes have turned to new practices using micro-doses and excess fluid ingestion to accelerate elimination and decrease the probability of detection. Our objective was to test the sensitivity of detection by validated methods (IEF: isoelectric focusing; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis) when such practices are used. First, after a three-week rhEPO boost period and 10 days of wash out, detection of a single 900 IU micro-dose of Eprex® was evaluated in healthy male subjects. After an injection in the evening, urine and plasma samples were collected the following morning. Half of the subjects then drank a bolus of water and new samples were collected 80 min later. Interestingly, rhEPO was detected in 100% of the samples even after water ingestion. A second similar protocol was then performed with a single injection of a micro-dose of rhEPO (500 IU or 900 IU), without a prior rhEPO boost. In addition, urine and plasma samples were also collected 15 and 20 h post rhEPO administration. Once again drinking water did not affect the rate of detection. Urine appeared a better matrix to detect micro-doses after 10 h, enabling between 92% and 100% of identification at that time. The rate of identification decreased rapidly thereafter, in particular for the 500 IU micro-dose. However IEF analysis still resulted in 71% identification of rhEPO in urine after 20 h. These results could help to define a better strategy for controlling and identifying athletes using rhEPO micro-doses. Copyright © 2016 John Wiley & Sons, Ltd.</div>
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